Spg003800.1 (mRNA) Sponge gourd (cylindrica) v1

BLAST of Spg003800.1 vs. NCBI nr
Match: XP_008457678.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.2 [Cucumis melo] >KAA0061599.1 protein NRT1/ PTR FAMILY 6.2 [Cucumis melo var. makuwa])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.9e-303
Identity = 546/625 (87.36%), Postives = 557/625 (89.12%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P  SKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLPQLHPPPCHPIGSKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKR MEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCA+W
Sbjct: 542 SLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLAILSAINFVAFLVCAVW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. NCBI nr
Match: TYJ97808.1 (protein NRT1/ PTR FAMILY 6.2 [Cucumis melo var. makuwa])

HSP 1 Score: 1050.8 bits (2716), Expect = 5.1e-303
Identity = 546/625 (87.36%), Postives = 557/625 (89.12%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P  SKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLPQLHPPPCHPIGSKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKR MEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRTMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCA+W
Sbjct: 542 SLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLAILSAINFVAFLVCAVW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. NCBI nr
Match: KAG6599405.1 (Protein NRT1/ PTR FAMILY 6.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.8e-301
Identity = 537/625 (85.92%), Postives = 558/625 (89.28%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           D KQSLTV+DAVDYKG PANRSKTGGWVPAALI+                          
Sbjct: 2   DEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLLCLLGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPCDP AS+NCK
Sbjct: 122 TCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCDPSASQNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTA+ IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKRKMELP NDA+LYED A TS +DHTNQFQ LDKAAIVAEGDFEKSVSSA NPWK
Sbjct: 302 VAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVAEGDFEKSVSSAHNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMM RLLPIWATTIIFWTTYAQMITFSV QASTMERSLG FQIPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTMERSLGAFQIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITL FYDRL+MPLWKKWKGQPGFTNLQ+IAIGLILSTLGMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILSTLGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQSPKGMK++STGLFLTTL
Sbjct: 482 AKAVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQSPKGMKSLSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL+ LSAINFVAFL+CAIW
Sbjct: 542 SLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLATLSAINFVAFLICAIW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+L + T+ NEGS AEKC
Sbjct: 602 YKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of Spg003800.1 vs. NCBI nr
Match: AFR11354.1 (nitrate transporter [Cucumis sativus])

HSP 1 Score: 1044.3 bits (2699), Expect = 4.8e-301
Identity = 543/625 (86.88%), Postives = 556/625 (88.96%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSA SANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSANSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P ASKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPIASKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYK SMGSPIVHIFQV+
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSPIVHIFQVL 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKRKMEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVA+GDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVADGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSL V
Sbjct: 362 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLPV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL E+KRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEIKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVK VTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCAIW
Sbjct: 542 SLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLTILSAINFVAFLVCAIW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. NCBI nr
Match: XP_022946201.1 (protein NRT1/ PTR FAMILY 6.2-like [Cucurbita moschata])

HSP 1 Score: 1044.3 bits (2699), Expect = 4.8e-301
Identity = 537/625 (85.92%), Postives = 558/625 (89.28%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           D KQSLTV+DAVDYKG PANRSKTGGWVPAALI+                          
Sbjct: 2   DEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLLCLLGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPCDP AS+NCK
Sbjct: 122 TCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCDPSASQNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTA+ IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKRKMELP NDA+LYED A TS +DHTNQFQ LDKAAIVAEGDFEKSV+SA NPWK
Sbjct: 302 VAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVAEGDFEKSVTSAHNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMM RLLPIWATTIIFWTTYAQMITFSV QASTMERSLG FQIPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTMERSLGAFQIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITL FYDRL+MPLWKKWKGQPGFTNLQ+IAIGLILSTLGMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILSTLGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQSPKGMK++STGLFLTTL
Sbjct: 482 AKAVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQSPKGMKSLSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL+ LSAINFVAFLVCAIW
Sbjct: 542 SLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLATLSAINFVAFLVCAIW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+L + T+ NEGS AEKC
Sbjct: 602 YKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of Spg003800.1 vs. ExPASy Swiss-Prot
Match: Q9SZY4 (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana OX=3702 GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.6e-238
Identity = 435/627 (69.38%), Postives = 494/627 (78.79%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           + K S TVADAVDYKG PA++SKTGGW+ AALIL                          
Sbjct: 2   ESKGSWTVADAVDYKGRPADKSKTGGWITAALIL-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIE+ ERLSTMGIAVNLVTYL+ T+HLPS+TSANIVTDFMG
Sbjct: 62  -------------------GIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           T FLLCLLGGFLADSFLGR+KTI IF+ IQ LGTG LAV TKLP+L PP C  H  + C 
Sbjct: 122 TSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC--HHGEACI 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
            A+ FQM ++Y+ LYLIALGTGG+KSS+SGFG+DQFD+KD KEKA MA+FFNRFF F+S 
Sbjct: 182 PATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISM 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVY+QDEVGRSWAYGIC+VSM  AI IFLCGTKRYRYKKS GSP+V IFQVI
Sbjct: 242 GTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVS--SAPNP 428
            AA  KRKMELP++   LYED+    RI+HT+QF  LDKAAIVAEGDFE+++   + PNP
Sbjct: 302 AAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLDGVAIPNP 361

Query: 429 WKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSL 488
           WKL SVT+VEEVKMM+RLLPIWATTIIFWTTYAQMITFSVEQASTM R++G+F+IPAGSL
Sbjct: 362 WKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSFKIPAGSL 421

Query: 489 TVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRL 548
           TVFFVAAILITLA YDR IMP WKKWKG+PGF++LQRIAIGL+LST GMAAAAL E KRL
Sbjct: 422 TVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLSTAGMAAAALVEQKRL 481

Query: 549 SVAEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLT 608
           SVA++   S  TLP+SVFLL+PQFFLVG+GEAFIYTGQLDFFITQSPKGMKTMSTGLFLT
Sbjct: 482 SVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMSTGLFLT 541

Query: 609 TLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCA 668
           TLSLGFFVSSFLV++VKRVT +    GWLADNIN+ RLD FY LL ILS INFV +++CA
Sbjct: 542 TLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFVVYIICA 577

Query: 669 IWYKPQKPKQILEIATSKNEGSVAEKC 694
           +W+KP K K  +E    K  G   E C
Sbjct: 602 LWFKPTKGKDSVEKENGK--GFSVEDC 577

BLAST of Spg003800.1 vs. ExPASy Swiss-Prot
Match: Q05085 (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana OX=3702 GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 3.1e-162
Identity = 313/625 (50.08%), Postives = 413/625 (66.08%), Query Frame = 0

Query: 67  LQDGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETR 126
           L + K    + DA D++G PA+RSKTGGW  AA+IL                        
Sbjct: 3   LPETKSDDILLDAWDFQGRPADRSKTGGWASAAMIL------------------------ 62

Query: 127 TSFKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDF 186
                                 IE  ERL+T+GI VNLVTYL GT+HL +AT+AN VT+F
Sbjct: 63  ---------------------CIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTNF 122

Query: 187 MGTCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKN 246
           +GT F+LCLLGGF+AD+FLGRY TIAIFA IQ  G   L + T +P L PP C+P  S +
Sbjct: 123 LGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTTSSH 182

Query: 247 CKQASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFV 306
           C+QASG Q+ ++YL LYL ALGTGG+K+SVSGFG+DQFDE + KE+++M YFFNRFF  +
Sbjct: 183 CEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCI 242

Query: 307 SSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQ 366
           + G+LLAVTVLVY+QD+VGR W YGIC+ ++  A+S+FL GT RYR+KK +GSP+  +  
Sbjct: 243 NVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAA 302

Query: 367 VIVAAVNKRKMELPRNDALLYE---------DSATTSRIDHTNQFQFLDKAAIVAEGDFE 426
           VIVAA   RK+ELP + + LY+               ++ HT QF+ LDKAAI    D E
Sbjct: 303 VIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI---RDQE 362

Query: 427 KSV-SSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSL 486
             V S+  N W L ++T VEEVK ++R+LPIWAT I+FWT +AQ+ T SV Q+ T++RS+
Sbjct: 363 AGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSI 422

Query: 487 GTFQIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILSTLGM 546
           G+F+IP  S+ VF+V  +L+T A YDR+ + L KK    P G   LQRI +GL   ++ M
Sbjct: 423 GSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAM 482

Query: 547 AAAALAEMKRLSVAEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKG 606
           A AAL E+KRL  A A G +  TLPL  +LLIPQ+ +VG GEA IYTGQLDFF+ + PKG
Sbjct: 483 AVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKG 542

Query: 607 MKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILS 666
           MK MSTGL L+TL+LGFF SS LV +V++ TG      W+AD++N  RL  FY L+++L 
Sbjct: 543 MKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK--AHPWIADDLNKGRLYNFYWLVAVLV 576

Query: 667 AINFVAFLVCAIWYKPQKPKQILEI 681
           A+NF+ FLV + WY   K K++ E+
Sbjct: 603 ALNFLIFLVFSKWY-VYKEKRLAEV 576

BLAST of Spg003800.1 vs. ExPASy Swiss-Prot
Match: Q9LVE0 (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana OX=3702 GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 4.9e-139
Identity = 285/618 (46.12%), Postives = 384/618 (62.14%), Query Frame = 0

Query: 78  DAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTSFKNVNRRFQ 137
           +A DY+G P ++SKTGGW+ A LIL                                   
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLIL----------------------------------- 75

Query: 138 VMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMGTCFLLCLLG 197
                     G EL ER+  MGI++NLVTYLVG LH+ SA SA IVT+FMGT  LL LLG
Sbjct: 76  ----------GSELSERICVMGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLG 135

Query: 198 GFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDP--HASKNCKQASGFQM 257
           GFLAD+ LGRYK +AI A +  LG   L V T +  + PP CD        C +A+G Q+
Sbjct: 136 GFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDFRRLHHQCIEANGHQL 195

Query: 258 GMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSSGTLLAVT 317
            ++Y+ LY IALG GGIKS+VSGFG+DQFD  D KE+ QM +FFNRF+  +S G+L AV 
Sbjct: 196 ALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVGSLFAVI 255

Query: 318 VLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVIVAAVNKR 377
            LVY+QD VGR W YGI + +M  A  + LCGTKRYR+KK  GSP   I++V   A  KR
Sbjct: 256 ALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGFLAWKKR 315

Query: 378 KMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAI------VAEGDFEKSVSSAPNPWKL 437
           K   P + +LL     TT  + HT   + LDKAAI       +  DFE+      +PW +
Sbjct: 316 KESYPAHPSLLNGYDNTT--VPHTEMLKCLDKAAISKNESSPSSKDFEEK-----DPWIV 375

Query: 438 CSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTVF 497
            +VT+VEEVK++M+L+PIWAT I+FWT Y+QM TF+VEQA+ M+R LG+F +PAGS + F
Sbjct: 376 STVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAF 435

Query: 498 FVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 557
            +  IL+  +  +R+ +PL ++   +P G T+LQRI +GL+ S   MA AA+ E  R   
Sbjct: 436 LILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAVAAVIENARREA 495

Query: 558 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 617
           A    +      +S F L+PQ+FLVG+GEAF Y GQL+FFI ++P+ MK+MSTGLFL+T+
Sbjct: 496 AVNNDKK-----ISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTI 555

Query: 618 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 677
           S+GFFVSS LV++V RVT     + WL  N+N ARL+ FY LL +L A+NF+ F+V A+ 
Sbjct: 556 SMGFFVSSLLVSLVDRVT----DKSWLRSNLNKARLNYFYWLLVVLGALNFLIFIVFAM- 570

Query: 678 YKPQKPKQILEIATSKNE 687
            K Q    ++ +  + ++
Sbjct: 616 -KHQYKADVITVVVTDDD 570

BLAST of Spg003800.1 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.1e-111
Identity = 231/616 (37.50%), Postives = 358/616 (58.12%), Query Frame = 0

Query: 67  LQDGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETR 126
           ++D K   T    +D    PAN++KTG W     IL                        
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFIL------------------------ 60

Query: 127 TSFKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDF 186
                                G E CERL+  G++ NL+ YL   +++ + +++  V+++
Sbjct: 61  ---------------------GTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNW 120

Query: 187 MGTCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKN 246
            GTC+   L+G F+AD++LGRY TIA F +I   G   L +   +P L  P C   + + 
Sbjct: 121 SGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTC---SGET 180

Query: 247 CKQASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFV 306
           C   +G Q  + ++ LYLIALGTGGIK  VS FG DQFD+ D+KEK   + FFN F+  +
Sbjct: 181 CHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVI 240

Query: 307 SSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQ 366
           + G ++A +VLV++Q  VG  W  G+ +V+M  A+  F  G+  YR +K  GSP+  + Q
Sbjct: 241 NVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQ 300

Query: 367 VIVAAVNKRKMELPRNDALLYEDSATTS------RIDHTNQFQFLDKAAIVAEGDFEKSV 426
           VIVA+  K K+++P +++LLYE+    S      +++HT    F DKAA+  E D + + 
Sbjct: 301 VIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAA 360

Query: 427 SSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLG-TF 486
            S  + WKLC+VT+VEE+K ++RLLPIWAT I+F + Y+QM T  V Q +T+++ +G  F
Sbjct: 361 KS--SSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNF 420

Query: 487 QIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKG-QPGFTNLQRIAIGLILSTLGMAAA 546
           +IP+ SL++F   ++L     YD+LI+P  +K+ G + GFT LQRI IGL++S   M +A
Sbjct: 421 KIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSA 480

Query: 547 ALAEMKRLSVAEAVG-RSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMK 606
            + E+ RL+  +     +  T+P+++F  +PQ+FLVG  E F + GQL+FF  Q+P  M+
Sbjct: 481 GILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMR 540

Query: 607 TMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAI 666
           ++ + L LT ++ G ++S+FLV +V +VT S    GW+A N+N   LD F+ LL+ LS +
Sbjct: 541 SLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGLSFL 564

Query: 667 NFVAFLVCAIWYKPQK 674
           NF+ +L  A WY  +K
Sbjct: 601 NFLVYLWIAKWYTYKK 564

BLAST of Spg003800.1 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.9e-111
Identity = 227/535 (42.43%), Postives = 331/535 (61.87%), Query Frame = 0

Query: 148 GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGR 207
           G E CERL+  GIA NL+TYL   LH  + ++A  VT + GTC+L  L+G  LAD++ GR
Sbjct: 53  GNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGR 112

Query: 208 YKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCKQASGFQMGMIYLPLYLIAL 267
           Y TIA F+ I  +G   L +   +P L P  C       C  A+  Q  M +  LYLIAL
Sbjct: 113 YWTIACFSGIYFIGMSALTLSASVPALKPAEC---IGDFCPSATPAQYAMFFGGLYLIAL 172

Query: 268 GTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRS 327
           GTGGIK  VS FG DQFD+ D +E+ + A FFN F+  ++ G L++ ++LV++Q+  G  
Sbjct: 173 GTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWG 232

Query: 328 WAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVIVAAVNKRKMELPRNDALLY 387
             +GI +V M  AI+ F  GT  YR++K  GSPI  I QV+VA+  K  +++P +  LLY
Sbjct: 233 LGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLY 292

Query: 388 EDS------ATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWKLCSVTRVEEVKMM 447
           E        A + +I+HT+  Q+LDKAA+++E   E       N W+LC+VT+VEE+K++
Sbjct: 293 ETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEE--ESKSGDYSNSWRLCTVTQVEELKIL 352

Query: 448 MRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTVFFVAAILITLAFY 507
           +R+ PIWA+ IIF   YAQM T  V+Q   M   +G+FQ+P  +L  F  A+++I +  Y
Sbjct: 353 IRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLY 412

Query: 508 DRLIMPLWKKWKG-QPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSVAEAVG--RSTAT 567
           DR I+PL +K+ G   GFT +QR+ IGL +S L MAAAA+ E+ RL +A  +G   S A 
Sbjct: 413 DRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAP 472

Query: 568 LPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFL 627
           +P+SV   IPQ+F++G+ E F + GQL+FF  QSP  M+++ + L L T +LG ++SS +
Sbjct: 473 VPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLI 532

Query: 628 VAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIWYKPQK 674
           + +V   T     +GW++DN+N   LD F+ LL+ LS +N   +   A  YK +K
Sbjct: 533 LTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Spg003800.1 vs. ExPASy TrEMBL
Match: A0A5A7V7F5 (Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001640 PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.9e-303
Identity = 546/625 (87.36%), Postives = 557/625 (89.12%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P  SKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLPQLHPPPCHPIGSKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKR MEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCA+W
Sbjct: 542 SLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLAILSAINFVAFLVCAVW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. ExPASy TrEMBL
Match: A0A1S3C670 (protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo OX=3656 GN=LOC103497318 PE=3 SV=1)

HSP 1 Score: 1051.2 bits (2717), Expect = 1.9e-303
Identity = 546/625 (87.36%), Postives = 557/625 (89.12%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P  SKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLPQLHPPPCHPIGSKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKR MEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRSMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCA+W
Sbjct: 542 SLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLAILSAINFVAFLVCAVW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. ExPASy TrEMBL
Match: A0A5D3BHY8 (Protein NRT1/ PTR FAMILY 6.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00180 PE=3 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 2.5e-303
Identity = 546/625 (87.36%), Postives = 557/625 (89.12%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P  SKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFATIQTLGTGTLAVITKLPQLHPPPCHPIGSKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKR MEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRTMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLP+WATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMMRLLPVWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVG STATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGISTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVKRVTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCA+W
Sbjct: 542 SLGFFVSSFLVAVVKRVTGSMDGQGWLADNINYARLDCFYGLLAILSAINFVAFLVCAVW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. ExPASy TrEMBL
Match: J7LKJ6 (Nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT1.4A PE=2 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.3e-301
Identity = 543/625 (86.88%), Postives = 556/625 (88.96%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           DGKQ LTVADAVDYKGCPANRSKTGGWVPAALI+                          
Sbjct: 2   DGKQRLTVADAVDYKGCPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSA SANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSANSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLL +LGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPC P ASKNCK
Sbjct: 122 TCFLLSMLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCHPIASKNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLY+IALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYIIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAI IFLCGTKRYRYK SMGSPIVHIFQV+
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAILIFLCGTKRYRYKISMGSPIVHIFQVL 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKRKMEL  N  LLYEDSA TSRIDHTNQFQFLDKAAIVA+GDFEKSVSSAPNPWK
Sbjct: 302 VAAINKRKMELQLNATLLYEDSAATSRIDHTNQFQFLDKAAIVADGDFEKSVSSAPNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSV QASTMERSLG F+IPAGSL V
Sbjct: 362 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVVQASTMERSLGNFKIPAGSLPV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILST GMAAAAL E+KRLSV
Sbjct: 422 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTFGMAAAALVEIKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL
Sbjct: 482 AKAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFFVSSFLVAVVK VTGS DGQGWLADNINYARLDCFYGLL+ILSAINFVAFLVCAIW
Sbjct: 542 SLGFFVSSFLVAVVKSVTGSMDGQGWLADNINYARLDCFYGLLTILSAINFVAFLVCAIW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+LE+ TS N GS AEKC
Sbjct: 602 YKPQKPKQLLEMETSTNGGSGAEKC 581

BLAST of Spg003800.1 vs. ExPASy TrEMBL
Match: A0A6J1G2Z7 (protein NRT1/ PTR FAMILY 6.2-like OS=Cucurbita moschata OX=3662 GN=LOC111450357 PE=3 SV=1)

HSP 1 Score: 1044.3 bits (2699), Expect = 2.3e-301
Identity = 537/625 (85.92%), Postives = 558/625 (89.28%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           D KQSLTV+DAVDYKG PANRSKTGGWVPAALI+                          
Sbjct: 2   DEKQSLTVSDAVDYKGFPANRSKTGGWVPAALIV-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG
Sbjct: 62  -------------------GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           TCFLLCLLGGFLADSFLGRYKTIAIFA IQTLGTGTLAVITKLPQLHPPPCDP AS+NCK
Sbjct: 122 TCFLLCLLGGFLADSFLGRYKTIAIFASIQTLGTGTLAVITKLPQLHPPPCDPSASQNCK 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
           QA+GFQMGMIYLPLYLIALGTGG+KSSVSGFGTDQFDEK+DKEKAQMAYFFNRFFLFVSS
Sbjct: 182 QANGFQMGMIYLPLYLIALGTGGLKSSVSGFGTDQFDEKNDKEKAQMAYFFNRFFLFVSS 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTA+ IFLCGTKRYRYKKSMGSPIVHIFQVI
Sbjct: 242 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAVMIFLCGTKRYRYKKSMGSPIVHIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWK 428
           VAA+NKRKMELP NDA+LYED A TS +DHTNQFQ LDKAAIVAEGDFEKSV+SA NPWK
Sbjct: 302 VAAINKRKMELPHNDAMLYEDPAATSTVDHTNQFQLLDKAAIVAEGDFEKSVTSAHNPWK 361

Query: 429 LCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTV 488
           LCSVTRVEEVKMM RLLPIWATTIIFWTTYAQMITFSV QASTMERSLG FQIPAGSLTV
Sbjct: 362 LCSVTRVEEVKMMTRLLPIWATTIIFWTTYAQMITFSVVQASTMERSLGAFQIPAGSLTV 421

Query: 489 FFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 548
           FFVAAILITL FYDRL+MPLWKKWKGQPGFTNLQ+IAIGLILSTLGMAAAAL EMKRLSV
Sbjct: 422 FFVAAILITLTFYDRLVMPLWKKWKGQPGFTNLQKIAIGLILSTLGMAAAALVEMKRLSV 481

Query: 549 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 608
           A+AVGRSTATLPLSVFLLIPQFFLVGS EAFIYTGQLDFFITQSPKGMK++STGLFLTTL
Sbjct: 482 AKAVGRSTATLPLSVFLLIPQFFLVGSAEAFIYTGQLDFFITQSPKGMKSLSTGLFLTTL 541

Query: 609 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 668
           SLGFF+SSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLL+ LSAINFVAFLVCAIW
Sbjct: 542 SLGFFLSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLATLSAINFVAFLVCAIW 581

Query: 669 YKPQKPKQILEIATSKNEGSVAEKC 694
           YKPQKPKQ+L + T+ NEGS AEKC
Sbjct: 602 YKPQKPKQVLAMETTTNEGSAAEKC 581

BLAST of Spg003800.1 vs. TAIR 10
Match: AT2G26690.1 (Major facilitator superfamily protein )

HSP 1 Score: 825.5 bits (2131), Expect = 3.2e-239
Identity = 435/627 (69.38%), Postives = 494/627 (78.79%), Query Frame = 0

Query: 69  DGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTS 128
           + K S TVADAVDYKG PA++SKTGGW+ AALIL                          
Sbjct: 2   ESKGSWTVADAVDYKGRPADKSKTGGWITAALIL-------------------------- 61

Query: 129 FKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMG 188
                              GIE+ ERLSTMGIAVNLVTYL+ T+HLPS+TSANIVTDFMG
Sbjct: 62  -------------------GIEVVERLSTMGIAVNLVTYLMETMHLPSSTSANIVTDFMG 121

Query: 189 TCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCK 248
           T FLLCLLGGFLADSFLGR+KTI IF+ IQ LGTG LAV TKLP+L PP C  H  + C 
Sbjct: 122 TSFLLCLLGGFLADSFLGRFKTIGIFSTIQALGTGALAVATKLPELRPPTC--HHGEACI 181

Query: 249 QASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSS 308
            A+ FQM ++Y+ LYLIALGTGG+KSS+SGFG+DQFD+KD KEKA MA+FFNRFF F+S 
Sbjct: 182 PATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISM 241

Query: 309 GTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVI 368
           GTLLAVTVLVY+QDEVGRSWAYGIC+VSM  AI IFLCGTKRYRYKKS GSP+V IFQVI
Sbjct: 242 GTLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVI 301

Query: 369 VAAVNKRKMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAIVAEGDFEKSVS--SAPNP 428
            AA  KRKMELP++   LYED+    RI+HT+QF  LDKAAIVAEGDFE+++   + PNP
Sbjct: 302 AAAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLDGVAIPNP 361

Query: 429 WKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSL 488
           WKL SVT+VEEVKMM+RLLPIWATTIIFWTTYAQMITFSVEQASTM R++G+F+IPAGSL
Sbjct: 362 WKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSFKIPAGSL 421

Query: 489 TVFFVAAILITLAFYDRLIMPLWKKWKGQPGFTNLQRIAIGLILSTLGMAAAALAEMKRL 548
           TVFFVAAILITLA YDR IMP WKKWKG+PGF++LQRIAIGL+LST GMAAAAL E KRL
Sbjct: 422 TVFFVAAILITLAVYDRAIMPFWKKWKGKPGFSSLQRIAIGLVLSTAGMAAAALVEQKRL 481

Query: 549 SVAEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLT 608
           SVA++   S  TLP+SVFLL+PQFFLVG+GEAFIYTGQLDFFITQSPKGMKTMSTGLFLT
Sbjct: 482 SVAKS--SSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMSTGLFLT 541

Query: 609 TLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCA 668
           TLSLGFFVSSFLV++VKRVT +    GWLADNIN+ RLD FY LL ILS INFV +++CA
Sbjct: 542 TLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFVVYIICA 577

Query: 669 IWYKPQKPKQILEIATSKNEGSVAEKC 694
           +W+KP K K  +E    K  G   E C
Sbjct: 602 LWFKPTKGKDSVEKENGK--GFSVEDC 577

BLAST of Spg003800.1 vs. TAIR 10
Match: AT1G12110.1 (nitrate transporter 1.1 )

HSP 1 Score: 573.5 bits (1477), Expect = 2.2e-163
Identity = 313/625 (50.08%), Postives = 413/625 (66.08%), Query Frame = 0

Query: 67  LQDGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETR 126
           L + K    + DA D++G PA+RSKTGGW  AA+IL                        
Sbjct: 3   LPETKSDDILLDAWDFQGRPADRSKTGGWASAAMIL------------------------ 62

Query: 127 TSFKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDF 186
                                 IE  ERL+T+GI VNLVTYL GT+HL +AT+AN VT+F
Sbjct: 63  ---------------------CIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTNF 122

Query: 187 MGTCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKN 246
           +GT F+LCLLGGF+AD+FLGRY TIAIFA IQ  G   L + T +P L PP C+P  S +
Sbjct: 123 LGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTTSSH 182

Query: 247 CKQASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFV 306
           C+QASG Q+ ++YL LYL ALGTGG+K+SVSGFG+DQFDE + KE+++M YFFNRFF  +
Sbjct: 183 CEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCI 242

Query: 307 SSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQ 366
           + G+LLAVTVLVY+QD+VGR W YGIC+ ++  A+S+FL GT RYR+KK +GSP+  +  
Sbjct: 243 NVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAA 302

Query: 367 VIVAAVNKRKMELPRNDALLYE---------DSATTSRIDHTNQFQFLDKAAIVAEGDFE 426
           VIVAA   RK+ELP + + LY+               ++ HT QF+ LDKAAI    D E
Sbjct: 303 VIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAI---RDQE 362

Query: 427 KSV-SSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSL 486
             V S+  N W L ++T VEEVK ++R+LPIWAT I+FWT +AQ+ T SV Q+ T++RS+
Sbjct: 363 AGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSI 422

Query: 487 GTFQIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILSTLGM 546
           G+F+IP  S+ VF+V  +L+T A YDR+ + L KK    P G   LQRI +GL   ++ M
Sbjct: 423 GSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAM 482

Query: 547 AAAALAEMKRLSVAEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKG 606
           A AAL E+KRL  A A G +  TLPL  +LLIPQ+ +VG GEA IYTGQLDFF+ + PKG
Sbjct: 483 AVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKG 542

Query: 607 MKTMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILS 666
           MK MSTGL L+TL+LGFF SS LV +V++ TG      W+AD++N  RL  FY L+++L 
Sbjct: 543 MKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGK--AHPWIADDLNKGRLYNFYWLVAVLV 576

Query: 667 AINFVAFLVCAIWYKPQKPKQILEI 681
           A+NF+ FLV + WY   K K++ E+
Sbjct: 603 ALNFLIFLVFSKWY-VYKEKRLAEV 576

BLAST of Spg003800.1 vs. TAIR 10
Match: AT3G21670.1 (Major facilitator superfamily protein )

HSP 1 Score: 496.5 bits (1277), Expect = 3.4e-140
Identity = 285/618 (46.12%), Postives = 384/618 (62.14%), Query Frame = 0

Query: 78  DAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETRTSFKNVNRRFQ 137
           +A DY+G P ++SKTGGW+ A LIL                                   
Sbjct: 16  EAYDYRGNPPDKSKTGGWLGAGLIL----------------------------------- 75

Query: 138 VMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMGTCFLLCLLG 197
                     G EL ER+  MGI++NLVTYLVG LH+ SA SA IVT+FMGT  LL LLG
Sbjct: 76  ----------GSELSERICVMGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLG 135

Query: 198 GFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDP--HASKNCKQASGFQM 257
           GFLAD+ LGRYK +AI A +  LG   L V T +  + PP CD        C +A+G Q+
Sbjct: 136 GFLADAKLGRYKMVAISASVTALGVLLLTVATTISSMRPPICDDFRRLHHQCIEANGHQL 195

Query: 258 GMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSSGTLLAVT 317
            ++Y+ LY IALG GGIKS+VSGFG+DQFD  D KE+ QM +FFNRF+  +S G+L AV 
Sbjct: 196 ALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVGSLFAVI 255

Query: 318 VLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVIVAAVNKR 377
            LVY+QD VGR W YGI + +M  A  + LCGTKRYR+KK  GSP   I++V   A  KR
Sbjct: 256 ALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGFLAWKKR 315

Query: 378 KMELPRNDALLYEDSATTSRIDHTNQFQFLDKAAI------VAEGDFEKSVSSAPNPWKL 437
           K   P + +LL     TT  + HT   + LDKAAI       +  DFE+      +PW +
Sbjct: 316 KESYPAHPSLLNGYDNTT--VPHTEMLKCLDKAAISKNESSPSSKDFEEK-----DPWIV 375

Query: 438 CSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTVF 497
            +VT+VEEVK++M+L+PIWAT I+FWT Y+QM TF+VEQA+ M+R LG+F +PAGS + F
Sbjct: 376 STVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAF 435

Query: 498 FVAAILITLAFYDRLIMPLWKKWKGQP-GFTNLQRIAIGLILSTLGMAAAALAEMKRLSV 557
            +  IL+  +  +R+ +PL ++   +P G T+LQRI +GL+ S   MA AA+ E  R   
Sbjct: 436 LILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAVAAVIENARREA 495

Query: 558 AEAVGRSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTL 617
           A    +      +S F L+PQ+FLVG+GEAF Y GQL+FFI ++P+ MK+MSTGLFL+T+
Sbjct: 496 AVNNDKK-----ISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTI 555

Query: 618 SLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIW 677
           S+GFFVSS LV++V RVT     + WL  N+N ARL+ FY LL +L A+NF+ F+V A+ 
Sbjct: 556 SMGFFVSSLLVSLVDRVT----DKSWLRSNLNKARLNYFYWLLVVLGALNFLIFIVFAM- 570

Query: 678 YKPQKPKQILEIATSKNE 687
            K Q    ++ +  + ++
Sbjct: 616 -KHQYKADVITVVVTDDD 570

BLAST of Spg003800.1 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 405.6 bits (1041), Expect = 8.0e-113
Identity = 231/616 (37.50%), Postives = 358/616 (58.12%), Query Frame = 0

Query: 67  LQDGKQSLTVADAVDYKGCPANRSKTGGWVPAALILGLHFVSSPPNAFLLLEVFLATETR 126
           ++D K   T    +D    PAN++KTG W     IL                        
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFIL------------------------ 60

Query: 127 TSFKNVNRRFQVMAKLIKSWTGIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDF 186
                                G E CERL+  G++ NL+ YL   +++ + +++  V+++
Sbjct: 61  ---------------------GTECCERLAYYGMSTNLINYLEKQMNMENVSASKSVSNW 120

Query: 187 MGTCFLLCLLGGFLADSFLGRYKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKN 246
            GTC+   L+G F+AD++LGRY TIA F +I   G   L +   +P L  P C   + + 
Sbjct: 121 SGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGL-TPTC---SGET 180

Query: 247 CKQASGFQMGMIYLPLYLIALGTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFV 306
           C   +G Q  + ++ LYLIALGTGGIK  VS FG DQFD+ D+KEK   + FFN F+  +
Sbjct: 181 CHATAG-QTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVI 240

Query: 307 SSGTLLAVTVLVYLQDEVGRSWAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQ 366
           + G ++A +VLV++Q  VG  W  G+ +V+M  A+  F  G+  YR +K  GSP+  + Q
Sbjct: 241 NVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQ 300

Query: 367 VIVAAVNKRKMELPRNDALLYEDSATTS------RIDHTNQFQFLDKAAIVAEGDFEKSV 426
           VIVA+  K K+++P +++LLYE+    S      +++HT    F DKAA+  E D + + 
Sbjct: 301 VIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAA 360

Query: 427 SSAPNPWKLCSVTRVEEVKMMMRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLG-TF 486
            S  + WKLC+VT+VEE+K ++RLLPIWAT I+F + Y+QM T  V Q +T+++ +G  F
Sbjct: 361 KS--SSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNF 420

Query: 487 QIPAGSLTVFFVAAILITLAFYDRLIMPLWKKWKG-QPGFTNLQRIAIGLILSTLGMAAA 546
           +IP+ SL++F   ++L     YD+LI+P  +K+ G + GFT LQRI IGL++S   M +A
Sbjct: 421 KIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSA 480

Query: 547 ALAEMKRLSVAEAVG-RSTATLPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMK 606
            + E+ RL+  +     +  T+P+++F  +PQ+FLVG  E F + GQL+FF  Q+P  M+
Sbjct: 481 GILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMR 540

Query: 607 TMSTGLFLTTLSLGFFVSSFLVAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAI 666
           ++ + L LT ++ G ++S+FLV +V +VT S    GW+A N+N   LD F+ LL+ LS +
Sbjct: 541 SLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGLSFL 564

Query: 667 NFVAFLVCAIWYKPQK 674
           NF+ +L  A WY  +K
Sbjct: 601 NFLVYLWIAKWYTYKK 564

BLAST of Spg003800.1 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 404.8 bits (1039), Expect = 1.4e-112
Identity = 227/535 (42.43%), Postives = 331/535 (61.87%), Query Frame = 0

Query: 148 GIELCERLSTMGIAVNLVTYLVGTLHLPSATSANIVTDFMGTCFLLCLLGGFLADSFLGR 207
           G E CERL+  GIA NL+TYL   LH  + ++A  VT + GTC+L  L+G  LAD++ GR
Sbjct: 53  GNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGR 112

Query: 208 YKTIAIFALIQTLGTGTLAVITKLPQLHPPPCDPHASKNCKQASGFQMGMIYLPLYLIAL 267
           Y TIA F+ I  +G   L +   +P L P  C       C  A+  Q  M +  LYLIAL
Sbjct: 113 YWTIACFSGIYFIGMSALTLSASVPALKPAEC---IGDFCPSATPAQYAMFFGGLYLIAL 172

Query: 268 GTGGIKSSVSGFGTDQFDEKDDKEKAQMAYFFNRFFLFVSSGTLLAVTVLVYLQDEVGRS 327
           GTGGIK  VS FG DQFD+ D +E+ + A FFN F+  ++ G L++ ++LV++Q+  G  
Sbjct: 173 GTGGIKPCVSSFGADQFDDTDSRERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWG 232

Query: 328 WAYGICSVSMFTAISIFLCGTKRYRYKKSMGSPIVHIFQVIVAAVNKRKMELPRNDALLY 387
             +GI +V M  AI+ F  GT  YR++K  GSPI  I QV+VA+  K  +++P +  LLY
Sbjct: 233 LGFGIPTVFMGLAIASFFFGTPLYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLY 292

Query: 388 EDS------ATTSRIDHTNQFQFLDKAAIVAEGDFEKSVSSAPNPWKLCSVTRVEEVKMM 447
           E        A + +I+HT+  Q+LDKAA+++E   E       N W+LC+VT+VEE+K++
Sbjct: 293 ETQDKNSAIAGSRKIEHTDDCQYLDKAAVISEE--ESKSGDYSNSWRLCTVTQVEELKIL 352

Query: 448 MRLLPIWATTIIFWTTYAQMITFSVEQASTMERSLGTFQIPAGSLTVFFVAAILITLAFY 507
           +R+ PIWA+ IIF   YAQM T  V+Q   M   +G+FQ+P  +L  F  A+++I +  Y
Sbjct: 353 IRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNCKIGSFQLPPAALGTFDTASVIIWVPLY 412

Query: 508 DRLIMPLWKKWKG-QPGFTNLQRIAIGLILSTLGMAAAALAEMKRLSVAEAVG--RSTAT 567
           DR I+PL +K+ G   GFT +QR+ IGL +S L MAAAA+ E+ RL +A  +G   S A 
Sbjct: 413 DRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAP 472

Query: 568 LPLSVFLLIPQFFLVGSGEAFIYTGQLDFFITQSPKGMKTMSTGLFLTTLSLGFFVSSFL 627
           +P+SV   IPQ+F++G+ E F + GQL+FF  QSP  M+++ + L L T +LG ++SS +
Sbjct: 473 VPISVLWQIPQYFILGAAEVFYFIGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLI 532

Query: 628 VAVVKRVTGSKDGQGWLADNINYARLDCFYGLLSILSAINFVAFLVCAIWYKPQK 674
           + +V   T     +GW++DN+N   LD F+ LL+ LS +N   +   A  YK +K
Sbjct: 533 LTLVTYFTTRNGQEGWISDNLNSGHLDYFFWLLAGLSLVNMAVYFFSAARYKQKK 582