Sgr028519.1 (mRNA) Monk fruit (Qingpiguo) v1

BLAST of Sgr028519.1 vs. NCBI nr
Match: XP_022144159.1 (cell wall protein RBR3 [Momordica charantia])

HSP 1 Score: 382.9 bits (982), Expect = 3.2e-102
Identity = 264/504 (52.38%), Postives = 299/504 (59.33%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           MPPSQS+KE+QVHS   S+SRAKNQ R+ASK PS  K++P  +S  S S   SG ASP  
Sbjct: 1   MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK 60

Query: 61  DASKPLSPAVAAAAPRSRVASKPPSPSQTSSRNHLHSKLTS-QLRLKADSPPSSSSQSAF 120
           DASKP SPAV  AAPR R++S PPSPSQTSSRNHL+ K TS Q +LKADS PSSSS+SAF
Sbjct: 61  DASKPSSPAV--AAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAF 120

Query: 121 PPQASSTLRSPSQENSRQQPLKKTSRVQSPSHLSSKPTGQSTS-QHIASPAAIEDQTTNG 180
            PQASST RSPSQ NS+QQP KKT             T QSTS QH A  AA EDQTTNG
Sbjct: 121 RPQASSTARSPSQANSKQQPSKKT-------------TAQSTSLQHTAFSAATEDQTTNG 180

Query: 181 IISHPANQLPQAKSKSKE------------------------------------------ 240
           + SH AN+  QA+ K +E                                          
Sbjct: 181 VTSHSANKSSQARPKGRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSE 240

Query: 241 -------------------------------------------------------KDRDS 300
                                                                  +DRDS
Sbjct: 241 NPTRSDQSIENGQDPSLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDS 300

Query: 301 EEKEIM-EG-ISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS------------- 360
           ++KE+M EG ISKAF KL I  SGEENPKSF++LIGDNKG SM+L+S             
Sbjct: 301 QKKELMGEGDISKAFPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRR 360

Query: 361 -----------------GNFKHKTPKDSRSEEDPPLRLCINNNLQGINNSILSNSSITER 374
                            GNF HKT +DSR+ EDPPLRL INNN QGINNSILSNSS TER
Sbjct: 361 EYQSNPDQSPKSSTEIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTER 420

BLAST of Sgr028519.1 vs. NCBI nr
Match: XP_023002262.1 (cell wall protein RBR3-like [Cucurbita maxima])

HSP 1 Score: 350.1 bits (897), Expect = 2.3e-92
Identity = 253/554 (45.67%), Postives = 288/554 (51.99%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           M PS+S K+A+VHSQPSSHSRAK QTR A+KPPS SK TP  S  ++KSP+ S  ASP H
Sbjct: 78  MLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSH 137

Query: 61  DASKPLSPA------------------------------------------------VAA 120
           DASKP S A                                                 AA
Sbjct: 138 DASKPSSSAGKVSPSHDTSKLSSPAGKGKVSPSRDTSMPSPPAGKAFPSRDASQPSSAAA 197

Query: 121 AAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSSSQSAFPPQASSTLRSPSQ 180
           AAPRS + SKPPSPSQTSS+NHLHSK TSQ RLKADS PSS S+ AF PQASS  RSPS 
Sbjct: 198 AAPRSHIRSKPPSPSQTSSKNHLHSKQTSQSRLKADSQPSSPSRPAFSPQASSIPRSPSH 257

Query: 181 ENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIA-SPAAIEDQTTNGIISHPANQLPQAK 240
           ENSRQQP KK SRVQSPSHLSSK T QSTSQ +  SPA I DQTT  ++SHPA+Q P+A+
Sbjct: 258 ENSRQQPSKKASRVQSPSHLSSKATAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRAR 317

Query: 241 SKSKE------------------------------------------------------- 300
            KSKE                                                       
Sbjct: 318 CKSKENQVQTKSKQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPLSNEDFSEIPIIID 377

Query: 301 ----------------------------------------------------------KD 360
                                                                     ++
Sbjct: 378 ETIENGPEPSLESQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPFEPEN 437

Query: 361 RDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS------------ 363
            DS+++  ME + KAFQ LNIK   EENPKSF +L GDNKGASMHL+S            
Sbjct: 438 SDSQQEGTMEDLPKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLISGEATKESSIHIH 497

BLAST of Sgr028519.1 vs. NCBI nr
Match: KAG6585587.1 (hypothetical protein SDJN03_18320, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349.7 bits (896), Expect = 3.0e-92
Identity = 256/555 (46.13%), Postives = 289/555 (52.07%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           M PS+S K+A+VHSQPSSHSRAK QTR A+KPPS SK TP  S  ++KSP+ S  ASP H
Sbjct: 70  MLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSH 129

Query: 61  DASKPLSPA------------------------------------------------VAA 120
           DASKP S A                                                 AA
Sbjct: 130 DASKPSSSAGKVSPSHDTSKLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAA 189

Query: 121 AAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSP-PSSSSQSAFPPQASSTLRSPS 180
           AAPRS++ SKPPSPSQTSS+NH  SK TSQ RLKADSP PSS S  AF PQASS  RSPS
Sbjct: 190 AAPRSQIRSKPPSPSQTSSKNHPQSKPTSQSRLKADSPQPSSPSWPAFSPQASSIPRSPS 249

Query: 181 QENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIA-SPAAIEDQTTNGIISHPANQLPQA 240
            ENSRQQP KK SRVQSPSHLSSKPT QSTSQ +  SPA IEDQTT  ++SHPA+Q P A
Sbjct: 250 HENSRQQPSKKASRVQSPSHLSSKPTAQSTSQQLTESPATIEDQTTKRVVSHPADQSPGA 309

Query: 241 KSKSKE------------------------------------------------------ 300
           + K KE                                                      
Sbjct: 310 RCKIKENQVQTKSKQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIII 369

Query: 301 -----------------------------------------------------------K 360
                                                                      +
Sbjct: 370 DETNENGPEPSLESQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPFEPE 429

Query: 361 DRDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS----------- 363
           + DS+++  ME +SKAFQ LNIK   EENPKSF +L GDNKGASMHL+S           
Sbjct: 430 NSDSQQEGTMEDLSKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESAIHI 489

BLAST of Sgr028519.1 vs. NCBI nr
Match: XP_022951875.1 (cell wall protein RBR3-like [Cucurbita moschata])

HSP 1 Score: 348.6 bits (893), Expect = 6.7e-92
Identity = 253/554 (45.67%), Postives = 289/554 (52.17%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           M PS+S K+A+VHSQPSSHSRAK QTR A+KPPS SK TP  S  ++KSP+ S  ASP H
Sbjct: 70  MLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSH 129

Query: 61  DASKPLSPA------------------------------------------------VAA 120
           DASKP S A                                                 AA
Sbjct: 130 DASKPSSSAGKVSPSHDTSKLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAA 189

Query: 121 AAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSSSQSAFPPQASSTLRSPSQ 180
           AAPRS++ SKPPSPSQTSS+NH  SK TSQ RLKADS PSS S+ AF PQASS  RSPS 
Sbjct: 190 AAPRSQIRSKPPSPSQTSSKNHPQSKPTSQSRLKADSQPSSPSRPAFSPQASSIPRSPSH 249

Query: 181 ENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIA-SPAAIEDQTTNGIISHPANQLPQAK 240
           ENSRQQP KK SRVQSPSHLSSKPT QSTSQ +  SPA I DQTT  ++SHPA+Q P+A+
Sbjct: 250 ENSRQQPSKKASRVQSPSHLSSKPTAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRAR 309

Query: 241 SKSKE------------------------------------------------------- 300
            K +E                                                       
Sbjct: 310 CKRRENQVQTKSKQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIIID 369

Query: 301 ----------------------------------------------------------KD 360
                                                                     ++
Sbjct: 370 ETIENGPETSLESQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPFEPEN 429

Query: 361 RDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS------------ 363
            DS+++  ME +SKAFQ LNIK   EENPKSF +L GDNKGASMHL+S            
Sbjct: 430 SDSQQEGTMEDLSKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESSIHIH 489

BLAST of Sgr028519.1 vs. NCBI nr
Match: KAG7020502.1 (hypothetical protein SDJN02_17187, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 343.2 bits (879), Expect = 2.8e-90
Identity = 253/555 (45.59%), Postives = 288/555 (51.89%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           M PS+S K+A+VHSQPSSHSRAK QTR A+KPPS SK TP  S  ++KSP+ S  ASP H
Sbjct: 70  MLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSH 129

Query: 61  DASKPLSPA------------------------------------------------VAA 120
           DASKP S A                                                 AA
Sbjct: 130 DASKPSSSAGKVSPSHDTSKLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAA 189

Query: 121 AAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSS-SQSAFPPQASSTLRSPS 180
           AAPRS++ SKPPSPSQTSS+NH  SK TSQ RLKADSP SSS S+ AF PQASS  RSPS
Sbjct: 190 AAPRSQIRSKPPSPSQTSSKNHPQSKPTSQSRLKADSPQSSSPSRPAFSPQASSIPRSPS 249

Query: 181 QENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIA-SPAAIEDQTTNGIISHPANQLPQA 240
            ENSRQQP KK S VQSPSHLSSKPT QSTSQ +  SPA I DQTT  ++SHPA+Q P A
Sbjct: 250 HENSRQQPSKKASLVQSPSHLSSKPTAQSTSQQLTESPATIGDQTTKRVVSHPADQSPGA 309

Query: 241 KSKSKE------------------------------------------------------ 300
           + K +E                                                      
Sbjct: 310 RCKIRENQVQTKSKQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIII 369

Query: 301 -----------------------------------------------------------K 360
                                                                      +
Sbjct: 370 DETNENGPEPSLESQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPFEPE 429

Query: 361 DRDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS----------- 363
           + DS+++  ME +SKAFQ LNIK   EENPKSF +L GDNKGASMHL+S           
Sbjct: 430 NSDSQQEGTMEDLSKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESAIHI 489

BLAST of Sgr028519.1 vs. ExPASy TrEMBL
Match: A0A6J1CRH0 (cell wall protein RBR3 OS=Momordica charantia OX=3673 GN=LOC111013918 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.6e-102
Identity = 264/504 (52.38%), Postives = 299/504 (59.33%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           MPPSQS+KE+QVHS   S+SRAKNQ R+ASK PS  K++P  +S  S S   SG ASP  
Sbjct: 1   MPPSQSKKESQVHSHSPSYSRAKNQPRIASKSPSLLKASPTVASNKSPSSPASGKASPSK 60

Query: 61  DASKPLSPAVAAAAPRSRVASKPPSPSQTSSRNHLHSKLTS-QLRLKADSPPSSSSQSAF 120
           DASKP SPAV  AAPR R++S PPSPSQTSSRNHL+ K TS Q +LKADS PSSSS+SAF
Sbjct: 61  DASKPSSPAV--AAPRPRISSVPPSPSQTSSRNHLNPKPTSKQSKLKADSQPSSSSRSAF 120

Query: 121 PPQASSTLRSPSQENSRQQPLKKTSRVQSPSHLSSKPTGQSTS-QHIASPAAIEDQTTNG 180
            PQASST RSPSQ NS+QQP KKT             T QSTS QH A  AA EDQTTNG
Sbjct: 121 RPQASSTARSPSQANSKQQPSKKT-------------TAQSTSLQHTAFSAATEDQTTNG 180

Query: 181 IISHPANQLPQAKSKSKE------------------------------------------ 240
           + SH AN+  QA+ K +E                                          
Sbjct: 181 VTSHSANKSSQARPKGRESQSQTKSKQSPKSKASKNQPQIKEELTSKNTSNPQSNQNSSE 240

Query: 241 -------------------------------------------------------KDRDS 300
                                                                  +DRDS
Sbjct: 241 NPTRSDQSIENGQDPSLESQAESKEDEETKEKATNASATSTLISSSESSSPYEEPEDRDS 300

Query: 301 EEKEIM-EG-ISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS------------- 360
           ++KE+M EG ISKAF KL I  SGEENPKSF++LIGDNKG SM+L+S             
Sbjct: 301 QKKELMGEGDISKAFPKLKINYSGEENPKSFITLIGDNKGTSMNLLSGKGNRESSIHIRR 360

Query: 361 -----------------GNFKHKTPKDSRSEEDPPLRLCINNNLQGINNSILSNSSITER 374
                            GNF HKT +DSR+ EDPPLRL INNN QGINNSILSNSS TER
Sbjct: 361 EYQSNPDQSPKSSTEIEGNFNHKTTEDSRTVEDPPLRLYINNNTQGINNSILSNSSFTER 420

BLAST of Sgr028519.1 vs. ExPASy TrEMBL
Match: A0A6J1KJ10 (cell wall protein RBR3-like OS=Cucurbita maxima OX=3661 GN=LOC111496164 PE=4 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 1.1e-92
Identity = 253/554 (45.67%), Postives = 288/554 (51.99%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           M PS+S K+A+VHSQPSSHSRAK QTR A+KPPS SK TP  S  ++KSP+ S  ASP H
Sbjct: 78  MLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSH 137

Query: 61  DASKPLSPA------------------------------------------------VAA 120
           DASKP S A                                                 AA
Sbjct: 138 DASKPSSSAGKVSPSHDTSKLSSPAGKGKVSPSRDTSMPSPPAGKAFPSRDASQPSSAAA 197

Query: 121 AAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSSSQSAFPPQASSTLRSPSQ 180
           AAPRS + SKPPSPSQTSS+NHLHSK TSQ RLKADS PSS S+ AF PQASS  RSPS 
Sbjct: 198 AAPRSHIRSKPPSPSQTSSKNHLHSKQTSQSRLKADSQPSSPSRPAFSPQASSIPRSPSH 257

Query: 181 ENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIA-SPAAIEDQTTNGIISHPANQLPQAK 240
           ENSRQQP KK SRVQSPSHLSSK T QSTSQ +  SPA I DQTT  ++SHPA+Q P+A+
Sbjct: 258 ENSRQQPSKKASRVQSPSHLSSKATAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRAR 317

Query: 241 SKSKE------------------------------------------------------- 300
            KSKE                                                       
Sbjct: 318 CKSKENQVQTKSKQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPLSNEDFSEIPIIID 377

Query: 301 ----------------------------------------------------------KD 360
                                                                     ++
Sbjct: 378 ETIENGPEPSLESQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPFEPEN 437

Query: 361 RDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS------------ 363
            DS+++  ME + KAFQ LNIK   EENPKSF +L GDNKGASMHL+S            
Sbjct: 438 SDSQQEGTMEDLPKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLISGEATKESSIHIH 497

BLAST of Sgr028519.1 vs. ExPASy TrEMBL
Match: A0A6J1GK50 (cell wall protein RBR3-like OS=Cucurbita moschata OX=3662 GN=LOC111454611 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 3.2e-92
Identity = 253/554 (45.67%), Postives = 289/554 (52.17%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           M PS+S K+A+VHSQPSSHSRAK QTR A+KPPS SK TP  S  ++KSP+ S  ASP H
Sbjct: 70  MLPSKSHKKAKVHSQPSSHSRAKKQTRTATKPPSASKVTPQSSVSSNKSPTTSAKASPSH 129

Query: 61  DASKPLSPA------------------------------------------------VAA 120
           DASKP S A                                                 AA
Sbjct: 130 DASKPSSSAGKVSPSHDTSKLSSPAGKGKVSPSHDTSKPSSPAGKAFPSRDASQPSSSAA 189

Query: 121 AAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSSSQSAFPPQASSTLRSPSQ 180
           AAPRS++ SKPPSPSQTSS+NH  SK TSQ RLKADS PSS S+ AF PQASS  RSPS 
Sbjct: 190 AAPRSQIRSKPPSPSQTSSKNHPQSKPTSQSRLKADSQPSSPSRPAFSPQASSIPRSPSH 249

Query: 181 ENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIA-SPAAIEDQTTNGIISHPANQLPQAK 240
           ENSRQQP KK SRVQSPSHLSSKPT QSTSQ +  SPA I DQTT  ++SHPA+Q P+A+
Sbjct: 250 ENSRQQPSKKASRVQSPSHLSSKPTAQSTSQQLTESPATIGDQTTKRVVSHPADQSPRAR 309

Query: 241 SKSKE------------------------------------------------------- 300
            K +E                                                       
Sbjct: 310 CKRRENQVQTKSKQSPKPDLKPVEFKASKHQPETMEEFISKNTSYPHSDQDFSEIPIIID 369

Query: 301 ----------------------------------------------------------KD 360
                                                                     ++
Sbjct: 370 ETIENGPETSLESQTESQESKEIKSYEEDLEKTTNALQINASKSKLITSAEITSPFEPEN 429

Query: 361 RDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVS------------ 363
            DS+++  ME +SKAFQ LNIK   EENPKSF +L GDNKGASMHL+S            
Sbjct: 430 SDSQQEGTMEDLSKAFQTLNIKYP-EENPKSFTTLTGDNKGASMHLLSGEATKESSIHIH 489

BLAST of Sgr028519.1 vs. ExPASy TrEMBL
Match: A0A6J1HD15 (pollen-specific leucine-rich repeat extensin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111462498 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 9.8e-73
Identity = 204/406 (50.25%), Postives = 251/406 (61.82%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           MPP      A V S+  SHSRAKNQTR+ SKPPSPSK+TP  S  ++KSP  SG ASP  
Sbjct: 72  MPP-----PANVRSKSPSHSRAKNQTRLVSKPPSPSKTTPQSSVDSNKSPLVSGKASPSQ 131

Query: 61  DA-SKPLSPAVAAAAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSSSQSAF 120
           +A SKP SP  AA APR R+ SK PSPS  +          SQ R  ADS P SSS+SAF
Sbjct: 132 EASSKPPSP--AATAPRRRIVSKSPSPSSQA----------SQSRFTADSQPPSSSRSAF 191

Query: 121 PPQASSTLRSPSQENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIASPAAIEDQT-TNG 180
            PQ        S EN  +     TS+  S  H S+K + ++  Q         DQT  NG
Sbjct: 192 SPQT-------SLENQPETKEDLTSKNNSNPH-SNKNSSKTPIQ--------SDQTIENG 251

Query: 181 IISHPANQLPQAKSKSKEKDRDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNK 240
           + + P +Q  Q+K   +  ++DS+++E ME ++KAFQKLNIK S +ENPKSF +LIG+NK
Sbjct: 252 LETSPESQ-EQSKETKENLEKDSKQEESMEDLAKAFQKLNIKYSDKENPKSFTTLIGNNK 311

Query: 241 GASMHLVS------------------------------GNFKHKTPKDSRSEEDPPLRLC 300
           GASMHL+S                              GNF HKTP+DSR+EE+P  +LC
Sbjct: 312 GASMHLLSGEPKADSSIHIHRQYKNNPVQIAKSFTEIEGNFNHKTPQDSRTEENPVQKLC 371

Query: 301 INNNLQGINNSILSNSSITERNPGVKLKFFREPTKSEDKSQPLHARKAKYTAKPAERLTY 360
           IN N+Q +NNSI+ +SS TE +PG+ LKF RE TKS+D+ Q          AKPA+R+T+
Sbjct: 372 ININVQDMNNSIMLDSSFTENDPGINLKFPRESTKSKDELQ---------VAKPAQRVTH 431

Query: 361 EPTVRRRCLRGLFMESSDSELDNPEKPRRHGCRYS-NCKGKEVEIL 374
           EP VRRRCLRGL MESSDSE+DNPEKPRRHGCRYS  CKGK VE L
Sbjct: 432 EPAVRRRCLRGLLMESSDSEVDNPEKPRRHGCRYSRGCKGKNVETL 434

BLAST of Sgr028519.1 vs. ExPASy TrEMBL
Match: A0A6J1K9X9 (probable serine/threonine-protein kinase samkC OS=Cucurbita maxima OX=3661 GN=LOC111492428 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 1.7e-72
Identity = 206/406 (50.74%), Postives = 252/406 (62.07%), Query Frame = 0

Query: 1   MPPSQSQKEAQVHSQPSSHSRAKNQTRMASKPPSPSKSTPPHSSVASKSPSPSGIASPLH 60
           MPP      A V S+  S SRAKNQTR  SKPPSPSK+TP  S  ++KSP   G ASP  
Sbjct: 72  MPP-----PANVRSKSPSQSRAKNQTRPVSKPPSPSKTTPQSSVDSNKSPLVLGKASPSQ 131

Query: 61  DA-SKPLSPAVAAAAPRSRVASKPPSPSQTSSRNHLHSKLTSQLRLKADSPPSSSSQSAF 120
           +A SKP SP  AA APR R+ASK PSPS  +          SQ R KADS P SSS+SAF
Sbjct: 132 EASSKPPSP--AATAPRRRIASKSPSPSSQA----------SQSRFKADSQPPSSSRSAF 191

Query: 121 PPQASSTLRSPSQENSRQQPLKKTSRVQSPSHLSSKPTGQSTSQHIASPAAIEDQT-TNG 180
            PQ        S EN  +     TS+  S  H S+K + ++  Q         DQT  NG
Sbjct: 192 SPQT-------SLENQPETKEDLTSKNNSNPH-SNKNSSKTPIQ--------SDQTIENG 251

Query: 181 IISHPANQLPQAKSKSKEKDRDSEEKEIMEGISKAFQKLNIKCSGEENPKSFVSLIGDNK 240
           + + P +Q  Q+K   +  ++DS+++E MEG++KAFQKLNIK S +ENPKSF +LIG+NK
Sbjct: 252 LETSPESQ-EQSKETKENLEKDSKQEESMEGLAKAFQKLNIKYSDKENPKSFTTLIGNNK 311

Query: 241 GASMHL------------------------------VSGNFKHKTPKDSRSEEDPPLRLC 300
           GASM L                              + GNFKHKTP+DSR+EE+P  +L 
Sbjct: 312 GASMLLHSGEPKADSSIHIHRQYKNNPGQIAKSFTEMEGNFKHKTPQDSRTEENPAQKLY 371

Query: 301 INNNLQGINNSILSNSSITERNPGVKLKFFREPTKSEDKSQPLHARKAKYTAKPAERLTY 360
           IN N+QG+NNSI+ +SS TE +PG+ LKF REPTKS+D+ Q          AKPAER+T+
Sbjct: 372 ININVQGMNNSIMLDSSFTENDPGINLKFPREPTKSKDELQ---------VAKPAERVTH 431

Query: 361 EPTVRRRCLRGLFMESSDSELDNPEKPRRHGCRYS-NCKGKEVEIL 374
           EP VRRRCLRGL MESSDSE+DNPEKPRRHGCRYS  CKGK+VE L
Sbjct: 432 EPAVRRRCLRGLLMESSDSEVDNPEKPRRHGCRYSRGCKGKKVETL 434

BLAST of Sgr028519.1 vs. TAIR 10
Match: AT1G75260.1 (oxidoreductases, acting on NADH or NADPH )

HSP 1 Score: 102.4 bits (254), Expect = 7.8e-22
Identity = 97/309 (31.39%), Postives = 141/309 (45.63%), Query Frame = 0

Query: 95  LHSKLTSQLRLKADSPPSSSSQSAFPPQASSTLRSPSQENSRQQPLKK-----TSRVQSP 154
           LH     +L        S  ++     +   +L++  +E++ +    +     +   + P
Sbjct: 210 LHDDGVQKLEADQGQQKSKETEKLALQETKRSLQAVGREDATRSKTTRHMAAASETTRGP 269

Query: 155 SHLSSKPTGQSTSQHIASPAAIEDQTTNG-IISHPANQLPQAKSKSKEKDRDSEEKEIME 214
             L  K T   T      P     Q T G + S+  N     +  S    R  +E +I +
Sbjct: 270 RDLPEKKT--ETQNRTEIPTDDNHQKTKGALTSNLGNPRVTNREGSSSMSRKIKE-DIRD 329

Query: 215 GISKAFQKLNIKCSGEENPKSFVSLIGDNKGASMHLVSGNFKH----------------- 274
           GISK       K +G++   S  +L G+NKGA+M + S   K                  
Sbjct: 330 GISKLTWG---KSNGDDKSVSVYTLTGENKGATMGIGSEKDKKDGEVHIRRGYRSNPDES 389

Query: 275 --------KTPKDSRSEEDPPLRLCINNNLQGINNSILSNSSITERNPGVKLKFFREPTK 334
                   + PKD  +EE+      IN N QGINNSI+  SS++E +PGV + F  E  K
Sbjct: 390 SNTTATETENPKDDEAEEEASFTAYINGNTQGINNSIVVESSVSENDPGVHMSFKFEILK 449

Query: 335 SEDKSQPLHARKAK-YTAKPAERLTYEPTVRRRCLRGLFMESSDSELDNPEKPRRHGCRY 372
            E    P +  + K  T    ++L  EP VRRRCLRGL  ESS+SE DNP KPRRHGCR+
Sbjct: 450 KEVIYPPENVEEKKPETVTVTKKLKNEPRVRRRCLRGLLAESSESEPDNPLKPRRHGCRF 509