Sgr027558.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr027558.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	DUF4408 domain protein
Location	tig00153054: 2516217 .. 2517146 (-)
Sequence length	930
RNA-Seq Expression	Sgr027558.1
Synteny	Sgr027558.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGATCTGTTACCAAAACCAAAACCGGTGTTTAAAGGTTTTACATTAGAGACGGCAATCTCGGTTACAAAATCGATACTGCTGTCAGTTGGGCTTATATCCACTGTAATTTTGTTCAAAGTGGCTATAATCCCAAATATATGGAATCTTCTGATCACTACTCTTCCTCGCTTTTGGGTCTCCTTCAGATTGTGGCTCTCTCCACCCTACGTCTTCATCGTCTTCAACTTCATTATTGTCGCCGTCGCTGCCTCCTCCACCTTTCGGCGCCAAAAAGACACACCCGACGATAATTATACCCCCATTTCTCATGAAAATACTCATGTTACTGCATCAACTACCTATAAATCGATGCATGAGGACCAAAACTTTTTGATCACTGCTCGCTCGAGAGAAATCTGGCGTGGGATCTCGCACGAAGATGAACAAGACGAACCTGGGAAAGAAGAAGAAGGAAAGCCGCTGAAATGGGATTCCCCGACTCTGTCCACCGAAAAATTCGGTGACCTATCATCGGAAAAGTTCTGCGCATATTCCGGTGAGAAGGTGGAGGAGCATGAAGATGCCGCCGGGGACTCGCTAGACGCGACGTGGAAGGCCATCATGGAAAGGCAAGGGAAACAGACGCCGCAGCTGAGAAAGAGCGAGACATGGGACTCCCCACCAGGCTTAATTAGAGCCGGCGCCGGCGCCGACGCCGACGCCGACGCAGTCCATGACGACGACCCGGTGGCGTGGGCTCGGAATGAGGTAAGGAAGTCGGACATATTCAAAGAAACGCTGTCGTTTAGGAGAAAGAAATCGATGAATCAAGAAGAACTGCAGCGGCGGGCCGAGGCATTCATAGAGATGGTAAACAAAAACATACGGTTACAGAGGCAAGAATCAGAGCAACGCGTCCTGCAGGCACTTAAACGCAGCGTTTAG

mRNA sequence

ATGGCTGATCTGTTACCAAAACCAAAACCGGTGTTTAAAGGTTTTACATTAGAGACGGCAATCTCGGTTACAAAATCGATACTGCTGTCAGTTGGGCTTATATCCACTGTAATTTTGTTCAAAGTGGCTATAATCCCAAATATATGGAATCTTCTGATCACTACTCTTCCTCGCTTTTGGGTCTCCTTCAGATTGTGGCTCTCTCCACCCTACGTCTTCATCGTCTTCAACTTCATTATTGTCGCCGTCGCTGCCTCCTCCACCTTTCGGCGCCAAAAAGACACACCCGACGATAATTATACCCCCATTTCTCATGAAAATACTCATGTTACTGCATCAACTACCTATAAATCGATGCATGAGGACCAAAACTTTTTGATCACTGCTCGCTCGAGAGAAATCTGGCGTGGGATCTCGCACGAAGATGAACAAGACGAACCTGGGAAAGAAGAAGAAGGAAAGCCGCTGAAATGGGATTCCCCGACTCTGTCCACCGAAAAATTCGGTGACCTATCATCGGAAAAGTTCTGCGCATATTCCGGTGAGAAGGTGGAGGAGCATGAAGATGCCGCCGGGGACTCGCTAGACGCGACGTGGAAGGCCATCATGGAAAGGCAAGGGAAACAGACGCCGCAGCTGAGAAAGAGCGAGACATGGGACTCCCCACCAGGCTTAATTAGAGCCGGCGCCGGCGCCGACGCCGACGCCGACGCAGTCCATGACGACGACCCGGTGGCGTGGGCTCGGAATGAGGTAAGGAAGTCGGACATATTCAAAGAAACGCTGTCGTTTAGGAGAAAGAAATCGATGAATCAAGAAGAACTGCAGCGGCGGGCCGAGGCATTCATAGAGATGGTAAACAAAAACATACGGTTACAGAGGCAAGAATCAGAGCAACGCGTCCTGCAGGCACTTAAACGCAGCGTTTAG

Coding sequence (CDS)

ATGGCTGATCTGTTACCAAAACCAAAACCGGTGTTTAAAGGTTTTACATTAGAGACGGCAATCTCGGTTACAAAATCGATACTGCTGTCAGTTGGGCTTATATCCACTGTAATTTTGTTCAAAGTGGCTATAATCCCAAATATATGGAATCTTCTGATCACTACTCTTCCTCGCTTTTGGGTCTCCTTCAGATTGTGGCTCTCTCCACCCTACGTCTTCATCGTCTTCAACTTCATTATTGTCGCCGTCGCTGCCTCCTCCACCTTTCGGCGCCAAAAAGACACACCCGACGATAATTATACCCCCATTTCTCATGAAAATACTCATGTTACTGCATCAACTACCTATAAATCGATGCATGAGGACCAAAACTTTTTGATCACTGCTCGCTCGAGAGAAATCTGGCGTGGGATCTCGCACGAAGATGAACAAGACGAACCTGGGAAAGAAGAAGAAGGAAAGCCGCTGAAATGGGATTCCCCGACTCTGTCCACCGAAAAATTCGGTGACCTATCATCGGAAAAGTTCTGCGCATATTCCGGTGAGAAGGTGGAGGAGCATGAAGATGCCGCCGGGGACTCGCTAGACGCGACGTGGAAGGCCATCATGGAAAGGCAAGGGAAACAGACGCCGCAGCTGAGAAAGAGCGAGACATGGGACTCCCCACCAGGCTTAATTAGAGCCGGCGCCGGCGCCGACGCCGACGCCGACGCAGTCCATGACGACGACCCGGTGGCGTGGGCTCGGAATGAGGTAAGGAAGTCGGACATATTCAAAGAAACGCTGTCGTTTAGGAGAAAGAAATCGATGAATCAAGAAGAACTGCAGCGGCGGGCCGAGGCATTCATAGAGATGGTAAACAAAAACATACGGTTACAGAGGCAAGAATCAGAGCAACGCGTCCTGCAGGCACTTAAACGCAGCGTTTAG

Protein sequence

MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMHEDQNFLITARSREIWRGISHEDEQDEPGKEEEGKPLKWDSPTLSTEKFGDLSSEKFCAYSGEKVEEHEDAAGDSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGADADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQRQESEQRVLQALKRSV

Homology

[Close]

BLAST of Sgr027558.1 vs. NCBI nr
Match: XP_022137281.1 (uncharacterized protein LOC111008778 [Momordica charantia])

HSP 1 Score: 399.8 bits (1026), Expect = 2.1e-107
Identity = 229/310 (73.87%), Postives = 249/310 (80.32%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MADL   PKPVF G TLETAISVTKSILL VGL+STVILFKVAIIP + +LLITT PR W
Sbjct: 1   MADL--SPKPVFNGLTLETAISVTKSILLFVGLVSTVILFKVAIIPKLVSLLITTPPRLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSF  WLSPPYVF VFNFIIVAVAASS FRRQKD PD NYTPISH       ST YKSMH
Sbjct: 61  VSFGFWLSPPYVFFVFNFIIVAVAASSAFRRQKDPPDGNYTPISH------PSTPYKSMH 120

Query: 121 EDQNFLITARSREIW-RGISHEDEQDEPGKEEEGKPLKWDSPTLSTEKFGDLSSEKFCAY 180
           EDQNF ITARSREIW  GI HED+Q+E GKEEEGKP+KWD    ST K  D  +EK CAY
Sbjct: 121 EDQNFSITARSREIWNNGILHEDDQNERGKEEEGKPVKWD---FSTGKVDDPLAEKLCAY 180

Query: 181 SGEKVEEHEDAAGDSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGADADADAV 240
           SGEKVEE ED  G+SLDATWKAIMERQGKQT QL+KS+TWDSPP LIRA A         
Sbjct: 181 SGEKVEEEED-DGESLDATWKAIMERQGKQTAQLKKSQTWDSPPRLIRAVADGG------ 240

Query: 241 HDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQRQESEQ 300
            ++D  AWARNEV+KS++FK+TLSFRR+ SM+ EEL+ RAEAFIEMVN+NIRLQRQESEQ
Sbjct: 241 -EEDAAAWARNEVKKSEMFKQTLSFRRRISMSSEELKSRAEAFIEMVNRNIRLQRQESEQ 291

Query: 301 RVLQALKRSV 310
           R LQALKRSV
Sbjct: 301 RFLQALKRSV 291

[Close]

BLAST of Sgr027558.1 vs. NCBI nr
Match: KAG6584433.1 (hypothetical protein SDJN03_20365, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 379.0 bits (972), Expect = 3.8e-101
Identity = 222/317 (70.03%), Postives = 249/317 (78.55%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MAD  P PKPV    TLETAISVTKSIL+SVG+IST+ILFKVA+IP +  L+ITTLPR W
Sbjct: 1   MAD--PSPKPV--SMTLETAISVTKSILVSVGIISTLILFKVAVIPKVATLVITTLPRLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSFRLWLSPPY+FIVFNFII AVAASS FRRQKD  ++NY  ISHENTHV AST YK  H
Sbjct: 61  VSFRLWLSPPYIFIVFNFIIFAVAASSIFRRQKDASENNY--ISHENTHVAASTPYKWTH 120

Query: 121 EDQNFLITARSREIWRGISHEDEQDEPGKE--EEGKPLKWDSPTLSTEKFGDLSSEKFCA 180
           ED+NF IT  S EIW  I  EDEQ +  +E  EE KP+KWD PTL TEKF D SS K CA
Sbjct: 121 EDRNFSITG-SIEIWDRIPSEDEQGKEVEEQKEEEKPVKWDFPTLFTEKFSDPSSGKSCA 180

Query: 181 YSGEKVEEHEDAAG-----DSLDATWKAIMERQGKQTPQLRKSETWDSPP--GLIRAGAG 240
           Y GEKVEE EDA G     DS+DATWKAIMER+GKQTPQL+KS+TWDSPP   LIRA   
Sbjct: 181 YPGEKVEEEEDAGGGGGDSDSMDATWKAIMEREGKQTPQLKKSQTWDSPPPARLIRAAVN 240

Query: 241 ADADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIR 300
           + AD     ++DPVAWARNEVRKS+ F +TLSFRRK S+  EEL+ RAEAFIEMVNK+IR
Sbjct: 241 SAAD-----EEDPVAWARNEVRKSERFHQTLSFRRKVSLTSEELKSRAEAFIEMVNKSIR 300

Query: 301 LQRQESEQRVLQALKRS 309
           LQRQESEQR LQA++RS
Sbjct: 301 LQRQESEQRFLQAMQRS 305

[Close]

BLAST of Sgr027558.1 vs. NCBI nr
Match: KAG7020020.1 (hypothetical protein SDJN02_18988, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 377.9 bits (969), Expect = 8.6e-101
Identity = 222/317 (70.03%), Postives = 249/317 (78.55%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MAD  P PKPV    TLETAISVTKSIL+SVG+IST+ILFKVA+IP +  L+ITTLPR W
Sbjct: 1   MAD--PSPKPV--SMTLETAISVTKSILVSVGIISTLILFKVAVIPKVATLVITTLPRLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSFRLWLSPPY+FIVFNFII AVAASS FRRQKD  ++NY  ISHENTHV AST YK  H
Sbjct: 61  VSFRLWLSPPYIFIVFNFIIFAVAASSIFRRQKDASENNY--ISHENTHVAASTPYKWTH 120

Query: 121 EDQNFLITARSREIWRGISHEDEQDEPGKE--EEGKPLKWDSPTLSTEKFGDLSSEKFCA 180
           ED+NF IT  S EIW  I  EDEQ +  +E  EE KP+KWD PTL TEKF D SS K CA
Sbjct: 121 EDRNFSITG-SIEIWDRIPSEDEQGKEVEEQKEEEKPVKWDFPTLFTEKFCDPSSGKSCA 180

Query: 181 YSGEKVEEHEDAAG-----DSLDATWKAIMERQGKQTPQLRKSETWDSPP--GLIRAGAG 240
           Y GEKVEE EDA G     DS+DATWKAIMER+GKQTPQL+KS+TWDSPP   LIRA   
Sbjct: 181 YPGEKVEEEEDAGGGGGDSDSMDATWKAIMEREGKQTPQLKKSQTWDSPPPARLIRAAVN 240

Query: 241 ADADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIR 300
           + AD     ++DPVAWARNEVRKS+ F +TLSFRRK S+  EEL+ RAEAFIEMVNK+IR
Sbjct: 241 SAAD-----EEDPVAWARNEVRKSERFHQTLSFRRKVSLTSEELKSRAEAFIEMVNKSIR 300

Query: 301 LQRQESEQRVLQALKRS 309
           LQRQESEQR LQA++RS
Sbjct: 301 LQRQESEQRFLQAMQRS 305

[Close]

BLAST of Sgr027558.1 vs. NCBI nr
Match: XP_022923785.1 (uncharacterized protein LOC111431395 [Cucurbita moschata])

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-100
Identity = 219/315 (69.52%), Postives = 247/315 (78.41%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MAD  P PKPV    TLETAISVTKSIL+SVG+IST+ILFKVA+IP +  L+ITTLPR W
Sbjct: 1   MAD--PSPKPV--SMTLETAISVTKSILVSVGIISTLILFKVAVIPKVATLVITTLPRLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSFRLWLSPPY+FIVFNFII AVAASS FRRQKD  ++NY  ISHENTHV AST YK  H
Sbjct: 61  VSFRLWLSPPYIFIVFNFIIFAVAASSIFRRQKDASENNY--ISHENTHVAASTPYKWTH 120

Query: 121 EDQNFLITARSREIWRGISHEDEQDEPGKE--EEGKPLKWDSPTLSTEKFGDLSSEKFCA 180
           ED+NF IT  S EIW  I  EDEQ +  +E  EE KP+KWD PTL TEKF D SS K CA
Sbjct: 121 EDRNFSITG-SIEIWDRIPSEDEQGKEVEEQKEEEKPVKWDFPTLFTEKFSDPSSGKLCA 180

Query: 181 YSGEKVEEHEDAAG-----DSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGAD 240
           Y GEKVEE EDA G     DS+DATWKAIMER+GKQTPQL+KS+TWDSPP      A  D
Sbjct: 181 YPGEKVEEEEDAGGGGGDSDSMDATWKAIMEREGKQTPQLKKSQTWDSPPPARLIRAAVD 240

Query: 241 ADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQ 300
           + AD   ++DPVAWARNEVRKS+ F +TLSFRRK S+  EEL+ RAEAFIEMVN++IRLQ
Sbjct: 241 SAAD---EEDPVAWARNEVRKSERFHQTLSFRRKVSLTSEELKSRAEAFIEMVNQSIRLQ 300

Query: 301 RQESEQRVLQALKRS 309
           RQESEQR LQA++RS
Sbjct: 301 RQESEQRFLQAMQRS 305

[Close]

BLAST of Sgr027558.1 vs. NCBI nr
Match: XP_023520184.1 (uncharacterized protein LOC111783484 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 373.6 bits (958), Expect = 1.6e-99
Identity = 219/315 (69.52%), Postives = 247/315 (78.41%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MAD  P PKPV    TLETAISVTKSIL+SVG+IST+ILFKVA+IP + +L+ITTLP  W
Sbjct: 1   MAD--PSPKPV--SMTLETAISVTKSILVSVGIISTLILFKVAVIPKVASLVITTLPGLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSFRLWLSPPY+FIVFNFII AVAASS FRRQKD  ++NY  ISHENTHV AST YK  H
Sbjct: 61  VSFRLWLSPPYIFIVFNFIIFAVAASSIFRRQKDASENNY--ISHENTHVAASTPYKWTH 120

Query: 121 EDQNFLITARSREIWRGISHEDEQDEPGKE-EEGKPLKWDSPTLSTEKFGDLSSEKFCAY 180
           ED+NF IT  S EIW  I  EDEQ   GKE EE KP+KWD PTL TEKF D SS K CAY
Sbjct: 121 EDRNFSITG-SIEIWDRIPTEDEQ---GKEVEEEKPVKWDFPTLFTEKFSDPSSGKLCAY 180

Query: 181 SGEKVEEHEDAAG------DSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGAD 240
            GEKVEE EDA G      DS+DATWKAIMER+GKQTPQL+KS+TWDSPP      A  D
Sbjct: 181 PGEKVEEEEDAGGGGGGDSDSMDATWKAIMEREGKQTPQLKKSQTWDSPPPARLIRAAVD 240

Query: 241 ADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQ 300
           + AD   ++DPVAWAR+EVRKS+ F +TLSFRRK S+  EEL+ RAEAFIEMVN++IRLQ
Sbjct: 241 SAAD---EEDPVAWARDEVRKSERFHQTLSFRRKVSLTSEELKSRAEAFIEMVNQSIRLQ 300

Query: 301 RQESEQRVLQALKRS 309
           RQESEQR LQA++RS
Sbjct: 301 RQESEQRFLQAMQRS 302

[Close]

BLAST of Sgr027558.1 vs. ExPASy TrEMBL
Match: A0A6J1C651 (uncharacterized protein LOC111008778 OS=Momordica charantia OX=3673 GN=LOC111008778 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 1.0e-107
Identity = 229/310 (73.87%), Postives = 249/310 (80.32%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MADL   PKPVF G TLETAISVTKSILL VGL+STVILFKVAIIP + +LLITT PR W
Sbjct: 1   MADL--SPKPVFNGLTLETAISVTKSILLFVGLVSTVILFKVAIIPKLVSLLITTPPRLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSF  WLSPPYVF VFNFIIVAVAASS FRRQKD PD NYTPISH       ST YKSMH
Sbjct: 61  VSFGFWLSPPYVFFVFNFIIVAVAASSAFRRQKDPPDGNYTPISH------PSTPYKSMH 120

Query: 121 EDQNFLITARSREIW-RGISHEDEQDEPGKEEEGKPLKWDSPTLSTEKFGDLSSEKFCAY 180
           EDQNF ITARSREIW  GI HED+Q+E GKEEEGKP+KWD    ST K  D  +EK CAY
Sbjct: 121 EDQNFSITARSREIWNNGILHEDDQNERGKEEEGKPVKWD---FSTGKVDDPLAEKLCAY 180

Query: 181 SGEKVEEHEDAAGDSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGADADADAV 240
           SGEKVEE ED  G+SLDATWKAIMERQGKQT QL+KS+TWDSPP LIRA A         
Sbjct: 181 SGEKVEEEED-DGESLDATWKAIMERQGKQTAQLKKSQTWDSPPRLIRAVADGG------ 240

Query: 241 HDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQRQESEQ 300
            ++D  AWARNEV+KS++FK+TLSFRR+ SM+ EEL+ RAEAFIEMVN+NIRLQRQESEQ
Sbjct: 241 -EEDAAAWARNEVKKSEMFKQTLSFRRRISMSSEELKSRAEAFIEMVNRNIRLQRQESEQ 291

Query: 301 RVLQALKRSV 310
           R LQALKRSV
Sbjct: 301 RFLQALKRSV 291

[Close]

BLAST of Sgr027558.1 vs. ExPASy TrEMBL
Match: A0A6J1ECW6 (uncharacterized protein LOC111431395 OS=Cucurbita moschata OX=3662 GN=LOC111431395 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 5.4e-101
Identity = 219/315 (69.52%), Postives = 247/315 (78.41%), Query Frame = 0


Query: 1   MADLLPKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFW 60
           MAD  P PKPV    TLETAISVTKSIL+SVG+IST+ILFKVA+IP +  L+ITTLPR W
Sbjct: 1   MAD--PSPKPV--SMTLETAISVTKSILVSVGIISTLILFKVAVIPKVATLVITTLPRLW 60

Query: 61  VSFRLWLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMH 120
           VSFRLWLSPPY+FIVFNFII AVAASS FRRQKD  ++NY  ISHENTHV AST YK  H
Sbjct: 61  VSFRLWLSPPYIFIVFNFIIFAVAASSIFRRQKDASENNY--ISHENTHVAASTPYKWTH 120

Query: 121 EDQNFLITARSREIWRGISHEDEQDEPGKE--EEGKPLKWDSPTLSTEKFGDLSSEKFCA 180
           ED+NF IT  S EIW  I  EDEQ +  +E  EE KP+KWD PTL TEKF D SS K CA
Sbjct: 121 EDRNFSITG-SIEIWDRIPSEDEQGKEVEEQKEEEKPVKWDFPTLFTEKFSDPSSGKLCA 180

Query: 181 YSGEKVEEHEDAAG-----DSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGAD 240
           Y GEKVEE EDA G     DS+DATWKAIMER+GKQTPQL+KS+TWDSPP      A  D
Sbjct: 181 YPGEKVEEEEDAGGGGGDSDSMDATWKAIMEREGKQTPQLKKSQTWDSPPPARLIRAAVD 240

Query: 241 ADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQ 300
           + AD   ++DPVAWARNEVRKS+ F +TLSFRRK S+  EEL+ RAEAFIEMVN++IRLQ
Sbjct: 241 SAAD---EEDPVAWARNEVRKSERFHQTLSFRRKVSLTSEELKSRAEAFIEMVNQSIRLQ 300

Query: 301 RQESEQRVLQALKRS 309
           RQESEQR LQA++RS
Sbjct: 301 RQESEQRFLQAMQRS 305

[Close]

BLAST of Sgr027558.1 vs. ExPASy TrEMBL
Match: A0A5A7T393 (DUF4408 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00490 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.3e-99
Identity = 210/309 (67.96%), Postives = 241/309 (77.99%), Query Frame = 0


Query: 6   PKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRL 65
           P  KPVF G TLETAIS+TKS+LL++G ISTVILFKVAIIP   +L I TLPR +VSFR 
Sbjct: 4   PSLKPVFHGLTLETAISITKSVLLAIGFISTVILFKVAIIPKFASLFIITLPRLFVSFRS 63

Query: 66  WLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMHEDQNF 125
           WLSPPYVFIVFNF++VA  ASS FRRQKDT +  +TPISHENT+V+ S  YK  HED++F
Sbjct: 64  WLSPPYVFIVFNFLMVAALASSVFRRQKDTSETKFTPISHENTNVSPSNPYKLTHEDRDF 123

Query: 126 LITARSREIWRGISHEDEQDEPGKEEEGKPLKWDSPTLSTEKFGDLSSEKFCAYSGEKVE 185
            IT RS EIW GIS EDEQD+  +EEE K LKWD PTL TEKF D SSEKFCAYS EKVE
Sbjct: 124 SITGRSGEIWNGISDEDEQDK--EEEEEKLLKWDFPTLFTEKFSDPSSEKFCAYSSEKVE 183

Query: 186 EHEDAA----GDSLDATWKAIMERQGKQTPQLRKSETWDS--PPGLIRAGAGADADADAV 245
           E +D       DS++ATWKAIMERQ KQTPQL+KS+TWDS  P  LIRA         AV
Sbjct: 184 EEKDGGDVGDDDSMEATWKAIMERQEKQTPQLKKSQTWDSPLPARLIRA---------AV 243

Query: 246 HDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQRQESEQ 305
            +++PVAW RNEVRK + F++TLSFRRK SM  EEL+ RAEAFIEMVN++IRLQRQESEQ
Sbjct: 244 REEEPVAWDRNEVRKPEKFQQTLSFRRKISMTSEELKSRAEAFIEMVNRSIRLQRQESEQ 301

Query: 306 RVLQALKRS 309
           R LQA+KRS
Sbjct: 304 RFLQAMKRS 301

[Close]

BLAST of Sgr027558.1 vs. ExPASy TrEMBL
Match: A0A1S4DS64 (uncharacterized protein LOC103503737 OS=Cucumis melo OX=3656 GN=LOC103503737 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.3e-99
Identity = 210/309 (67.96%), Postives = 241/309 (77.99%), Query Frame = 0


Query: 6   PKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRL 65
           P  KPVF G TLETAIS+TKS+LL++G ISTVILFKVAIIP   +L I TLPR +VSFR 
Sbjct: 4   PSLKPVFHGLTLETAISITKSVLLAIGFISTVILFKVAIIPKFASLFIITLPRLFVSFRS 63

Query: 66  WLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMHEDQNF 125
           WLSPPYVFIVFNF++VA  ASS FRRQKDT +  +TPISHENT+V+ S  YK  HED++F
Sbjct: 64  WLSPPYVFIVFNFLMVAALASSVFRRQKDTSETKFTPISHENTNVSPSNPYKLTHEDRDF 123

Query: 126 LITARSREIWRGISHEDEQDEPGKEEEGKPLKWDSPTLSTEKFGDLSSEKFCAYSGEKVE 185
            IT RS EIW GIS EDEQD+  +EEE K LKWD PTL TEKF D SSEKFCAYS EKVE
Sbjct: 124 SITGRSGEIWNGISDEDEQDK--EEEEEKLLKWDFPTLFTEKFSDPSSEKFCAYSSEKVE 183

Query: 186 EHEDAA----GDSLDATWKAIMERQGKQTPQLRKSETWDS--PPGLIRAGAGADADADAV 245
           E +D       DS++ATWKAIMERQ KQTPQL+KS+TWDS  P  LIRA         AV
Sbjct: 184 EEKDGGDVGDDDSMEATWKAIMERQEKQTPQLKKSQTWDSPLPARLIRA---------AV 243

Query: 246 HDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQRQESEQ 305
            +++PVAW RNEVRK + F++TLSFRRK SM  EEL+ RAEAFIEMVN++IRLQRQESEQ
Sbjct: 244 REEEPVAWDRNEVRKPEKFQQTLSFRRKISMTSEELKSRAEAFIEMVNRSIRLQRQESEQ 301

Query: 306 RVLQALKRS 309
           R LQA+KRS
Sbjct: 304 RFLQAMKRS 301

[Close]

BLAST of Sgr027558.1 vs. ExPASy TrEMBL
Match: A0A6J1KH91 (uncharacterized protein LOC111495736 OS=Cucurbita maxima OX=3661 GN=LOC111495736 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 8.6e-99
Identity = 212/308 (68.83%), Postives = 242/308 (78.57%), Query Frame = 0


Query: 6   PKPKPVFKGFTLETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRL 65
           P PKPV    TLETAISVTKSIL+SVG+IST+ILFKVA+IP +  L+I+TLPR WVSFRL
Sbjct: 4   PSPKPV--SMTLETAISVTKSILVSVGIISTLILFKVAVIPKVATLVISTLPRLWVSFRL 63

Query: 66  WLSPPYVFIVFNFIIVAVAASSTFRRQKDTPDDNYTPISHENTHVTASTTYKSMHEDQNF 125
           WLSPPY+FIVFNFII AVAASS FRRQKD  ++ Y  ISHENTHV AST YK  H+D+NF
Sbjct: 64  WLSPPYIFIVFNFIIFAVAASSIFRRQKDASENKY--ISHENTHVAASTPYKWTHDDRNF 123

Query: 126 LITARSREIWRGISHEDEQDEPGKE--EEGKPLKWDSPTLSTEKFGDLSSEKFCAYSGEK 185
            IT  S EIW  I  EDEQ +  +E  EE KP+KWD PTL TEKF D SS K CAY GEK
Sbjct: 124 SITG-SIEIWDRIPSEDEQGKQVEEQKEEEKPVKWDFPTLLTEKFSDPSSGKLCAYPGEK 183

Query: 186 VEEHEDAAG---DSLDATWKAIMERQGKQTPQLRKSETWDSPPGLIRAGAGADADADAVH 245
           VEE EDA G   DS+DATW AIMER+GKQTPQL+KS+TWDSPP      A  D+ AD   
Sbjct: 184 VEEEEDAGGGGSDSMDATWNAIMEREGKQTPQLKKSQTWDSPPPARLIRAAVDSAAD--- 243

Query: 246 DDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQRRAEAFIEMVNKNIRLQRQESEQR 305
           ++DPVAWARNEVRKS+ F +TLSFRRK S+  EEL+ RAEAFIEMVN++IRLQRQESEQR
Sbjct: 244 EEDPVAWARNEVRKSERFHQTLSFRRKVSLTSEELKSRAEAFIEMVNQSIRLQRQESEQR 303

Query: 306 VLQALKRS 309
            LQA++RS
Sbjct: 304 FLQAMQRS 303

[Close]

BLAST of Sgr027558.1 vs. TAIR 10
Match: AT1G61260.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 63.2 bits (152), Expect = 4.3e-10
Identity = 89/353 (25.21%), Postives = 144/353 (40.79%), Query Frame = 0


Query: 24  TKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRLWLSPPYVFIVFNFIIVAV 83
           TK++L+S G+ +  +L K++ +P   +  ++  P  W S   WL PPY+++V N II+ +
Sbjct: 8   TKAVLISSGVATVALLLKLS-VPVAVDFSVSRAPILWSSLLSWLKPPYLYVVTNGIIITI 67

Query: 84  AASSTFRR---QKDTPDD------------NYTPISHENTHVTASTTYKSMHEDQNF-LI 143
            ASS + R    +D  D+               PI +++         K +    +F  +
Sbjct: 68  VASSKYYRSHHDRDEEDEIVVYGGGGYKIQTEEPIVNQHQASPRILEVKDLDTGAHFGFV 127

Query: 144 TAR------SREIWRGISHEDEQD-----------EPGKEEEGK---------------- 203
            A         E    +  +DE++           E   EEE K                
Sbjct: 128 VANLEAEELESEAVTAVVFDDEEEEKKIIDSAATAEDEIEEELKSVIMVENSDLVESDVI 187

Query: 204 --PLKWDSPTLS-------TEKFGDLSSEKFCAYSGEKVEEHEDAAGDSLDATWKAIMER 263
             P+  +S  L        T +FG     K     G  +   +    ++L+ TWK I E 
Sbjct: 188 PPPMMIESENLPPIEKPLVTSRFGHRKLMKASQEGGRALRVTKPKKNETLENTWKMITE- 247

Query: 264 QGKQTPQLRKSETWDSPPGLIRAGAGADADADAVHDDDPVAWARNEVRKSDIFK------ 310
            GK TP  R+        G   +G G D +   V+            +KSD F+      
Sbjct: 248 -GKSTPLTRQLYRRSDTFGRGDSG-GVDGEVKPVY------------KKSDTFRDRTNYY 307

[Close]

BLAST of Sgr027558.1 vs. TAIR 10
Match: AT5G54300.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-08
Identity = 83/321 (25.86%), Postives = 138/321 (42.99%), Query Frame = 0


Query: 17  LETAISVTKSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRLWLSPPYVFIVF 76
           L T+   T + ++  G +S++    +  +P++ + +++  P  + +    L PPY+++V 
Sbjct: 5   LPTSSFKTTATVVIAG-VSSIATAMILTVPSVSHFVVSCFPIIYDNTVFLLKPPYLYLVI 64

Query: 77  NFIIVAVAASSTFRRQKDTPDDN-----YTPI--------------SHEN-THVTAS-TT 136
           N IIV + A+S    +  + DD+      TPI               + N  HV +  T 
Sbjct: 65  NSIIVCIIATSKLTHKSSSVDDSEISEVVTPIPIPVPVHLPSDIDSGYLNVVHVVSDYTG 124

Query: 137 YKSMHEDQNFLITARS----REIWRGISHEDEQDEPGKEEEGKPLKWDSPTLSTEKFGDL 196
           +    +D +   T  +     E+      ++  D P  E E   LK DSP +S  K    
Sbjct: 125 FVEKIDDVSINPTVEAIRKFPEVQEAEKSKESSDSPEPETEKPKLKNDSPEISILKHSTR 184

Query: 197 SSEKF----CAYSGEKVEEHEDAAG--------DSLDATWKAIMERQGKQTP---QLRKS 256
              +F       S  +    + A G        D+L+ TWK I E  G+ TP    L KS
Sbjct: 185 KPPRFNQQKSLKSNSEGGNKKTALGVTKPPRRQDTLETTWKKITE--GRSTPLTKHLTKS 244

Query: 257 ETWDSPPGLIRAGAGADADADAVHDDDPVAWARNEVRKSDIFKETLSFRRKKSMNQEELQ 298
           +TW       RA   +  +     + + +  + N    +   +E    +R+ S  QEEL 
Sbjct: 245 DTWQE-----RAHVQSSPE-----NKEKMTKSENLKDINTPTEEKTVLKREPSPGQEELN 304

[Close]

BLAST of Sgr027558.1 vs. TAIR 10
Match: AT1G11220.1 (Protein of unknown function (DUF761) )

HSP 1 Score: 42.4 bits (98), Expect = 7.9e-04
Identity = 82/325 (25.23%), Postives = 130/325 (40.00%), Query Frame = 0


Query: 25  KSILLSVGLISTVILFKVAIIPNIWNLLITTLPRFWVSFRLWLSPPYVFIVFNFIIVAVA 84
           K+ L++ G+++ V LF  + +P   +  ++  P FW SF  WL PPY+F+  N II  + 
Sbjct: 9   KAALITAGIVA-VSLFLKSSVPIAVDFSVSRFPIFWSSFLSWLKPPYLFVAINVIITIIM 68

Query: 85  ASSTFRR---QKDTPDDNYTPISHENTHVTAST-----TYKSMHEDQNFLITARSR---- 144
           ASS F +   ++D  DD    +  E T     T         +  D +F+ T +S     
Sbjct: 69  ASSKFYQSVGEQDGEDDEIL-LGGEYTIPNVITQAPPRRLVDLDADFDFVATVQSPILVA 128

Query: 145 --EIWRGISHEDEQDEPGKEEEGKPL-----KWDSPTL-STEKFGDLSSEKFCAYSGEKV 204
             EI   +  E E    G+   G        + + P +  +E           A SG + 
Sbjct: 129 EVEILEVVFEEKEMAISGQTNGGDEFAVMRSELNQPIMEESENLPPAEKPLVSARSGHRK 188

Query: 205 EEHEDAAG----------------DSLDATWKAIMERQGKQTP---QLRKSETWDSPPGL 264
                + G                ++L+ TW  I E +GK TP     RK+         
Sbjct: 189 PIKASSKGVNRKKKALKVVKPNRHETLENTWNMITE-EGKSTPLTCHYRKTS-------- 248

Query: 265 IRAGAGADADADAVHDDDPVAWARNEVRKSDIFKETLSFRR-----------KKSM--NQ 298
             +G  A  D   V            +RK++ F++  ++R+           KK M  ++
Sbjct: 249 -MSGLNAGGDVKPV------------LRKAETFRDVTNYRQSSPTVTSPVKMKKEMSPSR 305

The following BLAST results are available for this feature:

BLAST of Sgr027558.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137281.1	2.1e-107	73.87	uncharacterized protein LOC111008778 [Momordica charantia]	[more]
KAG6584433.1	3.8e-101	70.03	hypothetical protein SDJN03_20365, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7020020.1	8.6e-101	70.03	hypothetical protein SDJN02_18988, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022923785.1	1.1e-100	69.52	uncharacterized protein LOC111431395 [Cucurbita moschata]	[more]
XP_023520184.1	1.6e-99	69.52	uncharacterized protein LOC111783484 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr027558.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Sgr027558.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C651	1.0e-107	73.87	uncharacterized protein LOC111008778 OS=Momordica charantia OX=3673 GN=LOC111008...	[more]
A0A6J1ECW6	5.4e-101	69.52	uncharacterized protein LOC111431395 OS=Cucurbita moschata OX=3662 GN=LOC1114313...	[more]
A0A5A7T393	1.3e-99	67.96	DUF4408 domain protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2...	[more]
A0A1S4DS64	1.3e-99	67.96	uncharacterized protein LOC103503737 OS=Cucumis melo OX=3656 GN=LOC103503737 PE=...	[more]
A0A6J1KH91	8.6e-99	68.83	uncharacterized protein LOC111495736 OS=Cucurbita maxima OX=3661 GN=LOC111495736...	[more]

BLAST of Sgr027558.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61260.1	4.3e-10	25.21	Protein of unknown function (DUF761)	[more]
AT5G54300.1	1.8e-08	25.86	Protein of unknown function (DUF761)	[more]
AT1G11220.1	7.9e-04	25.23	Protein of unknown function (DUF761)	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025520	Domain of unknown function DUF4408	PFAM	PF14364	DUF4408	coord: 57..89 e-value: 3.4E-8 score: 33.1
IPR008480	Protein of unknown function DUF761, plant	PFAM	PF05553	DUF761	coord: 272..307 e-value: 2.3E-5 score: 23.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..166
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..157
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..238
None	No IPR available	PANTHER	PTHR33098:SF75	OS07G0563400 PROTEIN	coord: 24..305
None	No IPR available	PANTHER	PTHR33098	COTTON FIBER (DUF761)	coord: 24..305

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr027558	Sgr027558	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr027558.1.exon1	Sgr027558.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr027558.1	cds.Sgr027558.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr027558.1	Sgr027558.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane