Sgr027489.1 (mRNA) Monk fruit (Qingpiguo) v1

BLAST of Sgr027489.1 vs. NCBI nr
Match: XP_022137034.1 (RHOMBOID-like protein 12, mitochondrial [Momordica charantia])

HSP 1 Score: 600.1 bits (1546), Expect = 1.2e-167
Identity = 302/336 (89.88%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNKLFSLKLASR+SNSTATS PCK+CTSFATL+RTHNQNHLFGSFI+RP+QDHSHSWSS 
Sbjct: 1   MNKLFSLKLASRISNSTATSRPCKNCTSFATLVRTHNQNHLFGSFISRPLQDHSHSWSSQ 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSFA KIHG FS  V+GKRFLL SSNTL K SIRSLI   +LFTGAQFSKLRFQLKPGFD
Sbjct: 61  RSFAEKIHGLFSVSVVGKRFLLNSSNTLFKGSIRSLIERRFLFTGAQFSKLRFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
           FQWRGRR WFQRL++ND+VLGLIIANVAVF LWRIADP+FMS+NFTISVEN RSGR+HTL
Sbjct: 121 FQWRGRRSWFQRLTSNDVVLGLIIANVAVFLLWRIADPVFMSRNFTISVENIRSGRVHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+EVGHIVSNMIGLYFFGMNIGRVFG EFLLRLYLAGAIGGS FYL HHLFQAS
Sbjct: 181 ITSAFSHVEVGHIVSNMIGLYFFGMNIGRVFGSEFLLRLYLAGAIGGSAFYLVHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK RPFWGKDPIRAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKDRPFWGKDPIRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 336

BLAST of Sgr027489.1 vs. NCBI nr
Match: XP_022923619.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita moschata])

HSP 1 Score: 565.1 bits (1455), Expect = 4.1e-157
Identity = 285/336 (84.82%), Postives = 304/336 (90.48%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNK+FS KLASRLSNS   SH C+DC SFAT +RT NQN+ +GSFITRPV+DHS SWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSF  KIHGFFSGPV+GKRF+L  SNTL K SI+SL+    LF G+ FSKL+FQLKPGFD
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRS-LFMGSPFSKLKFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            +W GRR WFQRL+TND+VLGLIIANVAVF LWRIADPIFMSKNFTISVEN RSGRIHTL
Sbjct: 121 HRWSGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+E+GHIVSNMIGLYFFGMNIGRVFGPEFLL LYLAGAIGGS FYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SS+ RPFWGKDP RAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sgr027489.1 vs. NCBI nr
Match: KAG7019987.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 563.9 bits (1452), Expect = 9.2e-157
Identity = 285/336 (84.82%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNK FS KLASRLSNS   SH C+DC SFAT +RT NQN+ +GSFITRPV+DHS SWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSF  KIHGFFSGPV+GKRF+L  SNTL K SI+SL+    LF G+ FSKL+FQLKPGFD
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRS-LFMGSPFSKLKFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            +W GRR WFQRL+TND+VLGLIIANVAVF LWRIADPIFMSKNFTISVEN RSGRIHTL
Sbjct: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+E+GHIVSNMIGLYFFGMNIGRVFGPEFLL LYLAGAIGGS FYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SS+ RPFWGKDP RAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sgr027489.1 vs. NCBI nr
Match: XP_038893963.1 (RHOMBOID-like protein 12, mitochondrial [Benincasa hispida])

HSP 1 Score: 561.2 bits (1445), Expect = 5.9e-156
Identity = 285/336 (84.82%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNKLFS KLASRLSNS A S  CKDC SFATL+RTHNQNHLF S +TRP+QD S SWSSH
Sbjct: 1   MNKLFSRKLASRLSNSFANSQHCKDCASFATLVRTHNQNHLFASSLTRPLQDRSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSFA KIHGF S  V+GKRF+L  SNTL K SI+SL+ +  LFTG  FSKLRFQLKPGFD
Sbjct: 61  RSFAEKIHGFSSVAVVGKRFILNPSNTLFKGSIKSLVESSSLFTGTPFSKLRFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            QW GR+ WFQRL+ ND+VLGLIIANVAVF LW+IADPIFMSKNFTIS+EN RSGRIHTL
Sbjct: 121 RQWGGRQSWFQRLTPNDVVLGLIIANVAVFLLWQIADPIFMSKNFTISIENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH E+GHIVSNMIGLYFFGMNIGRVFGPEFLL+LYLAGAIGGS FYLAH+LFQAS
Sbjct: 181 ITSAFSHKEIGHIVSNMIGLYFFGMNIGRVFGPEFLLKLYLAGAIGGSAFYLAHNLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK RPFWGKDP+RAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 336

BLAST of Sgr027489.1 vs. NCBI nr
Match: XP_023001193.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita maxima])

HSP 1 Score: 560.5 bits (1443), Expect = 1.0e-155
Identity = 284/336 (84.52%), Postives = 302/336 (89.88%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNK FS KLASRLSNS   SH C+DC SFAT +RT NQN+ +GSFITRPV+DHS SWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RS   KIHGFFSGPV+GKRF+L  S+TL K SI+SL+    LF G+ FSKL+FQLKPGFD
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVERS-LFMGSPFSKLKFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            +W GRR WFQRL+TND+VLGLIIANVAVF LWRIADPIFMSKNFTISVEN RSGRIHTL
Sbjct: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+E+GHIVSNMIGLYFFGMNIGRVFGPEFLL LYLAGAIGGS FYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK RPFWGKDP RAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sgr027489.1 vs. ExPASy Swiss-Prot
Match: Q9FZ81 (RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.0e-86
Identity = 183/339 (53.98%), Postives = 231/339 (68.14%), Query Frame = 0

Query: 6   SLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFI-----TRPVQ--DHSHSWS 65
           S +L   L+N T  SH  +   +F +L + +   H    ++     + PV   D S  W 
Sbjct: 14  SSRLTKLLANPTTHSHLNRQ--TFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWR 73

Query: 66  SHRSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPG 125
           S      KI GFF+  +  K            V + +L+ +   F G+QF K  F+ +  
Sbjct: 74  SE-----KIRGFFASALGNK-----------AVKLGNLVESRVGFIGSQFPKKGFEFQRF 133

Query: 126 FDFQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIH 185
             FQ RG + W Q LS  D+VLGL+IAN  VF +WR+ +  FM  NF IS++N +SGR+H
Sbjct: 134 SGFQRRGWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLH 193

Query: 186 TLITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQ 245
           TLITSAFSHI++GHIVSNMIGLYFFG +I R FGP+FLL+LYLAGA+GGSVFYL HH + 
Sbjct: 194 TLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGSVFYLIHHAYM 253

Query: 246 ASSS-KGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL 305
           A++S KG+  + +DP R  GLGASGAVNAIMLLDI L P++TLYLEFFIPVPA+LLGIFL
Sbjct: 254 AATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIPVPAMLLGIFL 313

Query: 306 IGKDMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           IGKD+LRI E +S ISGSAHLGGAAVAAIAWAR+R+GRF
Sbjct: 314 IGKDILRITEGNSNISGSAHLGGAAVAAIAWARIRKGRF 334

BLAST of Sgr027489.1 vs. ExPASy Swiss-Prot
Match: Q84WG3 (Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 3.0e-49
Identity = 116/281 (41.28%), Postives = 169/281 (60.14%), Query Frame = 0

Query: 62  SFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFDF 121
           S + ++HGFF+G +          NT  K+   +++ +   F  ++     F+      F
Sbjct: 52  SRSARVHGFFAGKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRI--ADPIFMSKNFTISVENCRSGRIHT 181
           Q RG + W      N +V GL+IAN AVF +WR+   D ++M KNF +S  +  +GRIHT
Sbjct: 112 QKRGWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIHT 171

Query: 182 LITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQA 241
           LITS FSH+   HI+ NM+GL +FG  I R FGP +LL+LY AGA+GGSVF+L+ H    
Sbjct: 172 LITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHALSV 231

Query: 242 SSSKGRPFWGKDPIRAQ--GLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL 301
            S KG+    KD ++     LGA+G V AI LLD+LL+PK T Y    + VP +  GI+ 
Sbjct: 232 ISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAGIYS 291

Query: 302 IGKDMLRILE--NDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           +G +++++LE  N++ ++    LGG  VA IAWAR+R+GRF
Sbjct: 292 LGLNIIKMLEGKNNNTLTSLDQLGGVVVAVIAWARIRKGRF 320

BLAST of Sgr027489.1 vs. ExPASy Swiss-Prot
Match: B3H707 (Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 7.3e-40
Identity = 115/310 (37.10%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 34  RTHNQNHLFGSFITRPVQDHSHSWSSHRSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSI 93
           + +   HL  S ++ P     H   S  S + K+HGFF+  +          NT  K+  
Sbjct: 31  KPNQSRHLLPSRLSSP-SSVPHFVPSAVSRSAKVHGFFASKL---------GNTNLKLKF 90

Query: 94  RSLIANMYLFTGAQFSKLRFQLKPGFDFQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLW 153
            +++ +   F  ++     F+      FQ RG + W      N +V GL+IAN AVF +W
Sbjct: 91  GNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIANAAVFTMW 150

Query: 154 RIADPIFMSKNFTISVENCRSGRIHTLITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGP 213
           R++D     +++ +S  +  SG IHTLITS FSHI    I+ NMIG+ +FG  I R  GP
Sbjct: 151 RVSD-----RSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTLGP 210

Query: 214 EFLLRLYLAGAIGGSVFYLAHHLFQASSSKGRPFWGKD-----PIRAQGLGASGAVNAIM 273
            +LL+LY AGA+GGSV +L++H   A + KG     KD     PI +Q LGA G++ AI 
Sbjct: 211 LYLLKLYFAGALGGSVCFLSYHALLA-TLKGEGVVIKDHQSTAPI-SQLLGADGSMFAIA 270

Query: 274 LLDILLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQ--ISGSAHLGGAAVAAI 333
           LLD+ ++PK T Y    + V  ++  I  +G ++L I E       S S  LGG  VAA+
Sbjct: 271 LLDMFIYPKVTTYFALMLRV-HVMFRIINLGVEILNIPEGGPNHIASSSGQLGGVVVAAM 320

Query: 334 AWARVRRGRF 337
           AWAR+++GRF
Sbjct: 331 AWARIKKGRF 320

BLAST of Sgr027489.1 vs. ExPASy Swiss-Prot
Match: P53259 (Rhomboid protein 1, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=PCP1 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-08
Identity = 55/192 (28.65%), Postives = 90/192 (46.88%), Query Frame = 0

Query: 137 DMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTLITSAFSHIEVGHIVSN 196
           ++V  L+  NVAVF LW++       + + +  ++  + +I ++I SAFSH E  H+  N
Sbjct: 144 NLVYALLGINVAVFGLWQLPKCWRFLQKYMLLQKDYVTSKI-SIIGSAFSHQEFWHLGMN 203

Query: 197 MIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQASSSKGRPFWGKDPIRAQ 256
           M+ L+ FG ++  + G      LY+  AI GS+F L +           P   +  I   
Sbjct: 204 MLALWSFGTSLATMLGASNFFSLYMNSAIAGSLFSLWY-----------PKLARLAIVGP 263

Query: 257 GLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL--IGKDMLRILENDSQISG 316
            LGASGA+  ++     LFP + + L F  PVP      FL  +  +             
Sbjct: 264 SLGASGALFGVLGCFSYLFPHAKILL-FVFPVPGGAWVAFLASVAWNAAGCALRWGSFDY 322

Query: 317 SAHLGGAAVAAI 327
           +AHLGG+ +  +
Sbjct: 324 AAHLGGSMMGVL 322

BLAST of Sgr027489.1 vs. ExPASy Swiss-Prot
Match: Q9H300 (Presenilins-associated rhomboid-like protein, mitochondrial OS=Homo sapiens OX=9606 GN=PARL PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 1.1e-06
Identity = 58/199 (29.15%), Postives = 85/199 (42.71%), Query Frame = 0

Query: 129 WFQRLSTND-MVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRI-HTLITSAFS 188
           W+  LS     V G+I ANV VF LWR+  P            N  S  +   ++ S FS
Sbjct: 158 WWNNLSDGQRTVTGIIAANVLVFCLWRV--PSLQRTMIRYFTSNPASKVLCSPMLLSTFS 217

Query: 189 HIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYL-AGAIGGSVFYLAHHLFQASSSKGR 248
           H  + H+ +NM  L+ F  +I  + G E  + +YL AG I   V Y+           G+
Sbjct: 218 HFSLFHMAANMYVLWSFSSSIVNILGQEQFMAVYLSAGVISNFVSYV-----------GK 277

Query: 249 PFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL---IGKDM 308
              G+       LGASGA+  ++       P+  L +  F+P+     G  L   I  D 
Sbjct: 278 VATGR---YGPSLGASGAIMTVLAAVCTKIPEGRLAI-IFLPMFTFTAGNALKAIIAMDT 337

Query: 309 LRILENDSQISGSAHLGGA 322
             ++        +AHLGGA
Sbjct: 338 AGMILGWKFFDHAAHLGGA 339

BLAST of Sgr027489.1 vs. ExPASy TrEMBL
Match: A0A6J1C609 (RHOMBOID-like protein 12, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111008595 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.6e-168
Identity = 302/336 (89.88%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNKLFSLKLASR+SNSTATS PCK+CTSFATL+RTHNQNHLFGSFI+RP+QDHSHSWSS 
Sbjct: 1   MNKLFSLKLASRISNSTATSRPCKNCTSFATLVRTHNQNHLFGSFISRPLQDHSHSWSSQ 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSFA KIHG FS  V+GKRFLL SSNTL K SIRSLI   +LFTGAQFSKLRFQLKPGFD
Sbjct: 61  RSFAEKIHGLFSVSVVGKRFLLNSSNTLFKGSIRSLIERRFLFTGAQFSKLRFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
           FQWRGRR WFQRL++ND+VLGLIIANVAVF LWRIADP+FMS+NFTISVEN RSGR+HTL
Sbjct: 121 FQWRGRRSWFQRLTSNDVVLGLIIANVAVFLLWRIADPVFMSRNFTISVENIRSGRVHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+EVGHIVSNMIGLYFFGMNIGRVFG EFLLRLYLAGAIGGS FYL HHLFQAS
Sbjct: 181 ITSAFSHVEVGHIVSNMIGLYFFGMNIGRVFGSEFLLRLYLAGAIGGSAFYLVHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK RPFWGKDPIRAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKDRPFWGKDPIRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 336

BLAST of Sgr027489.1 vs. ExPASy TrEMBL
Match: A0A6J1E6X7 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111431274 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 285/336 (84.82%), Postives = 304/336 (90.48%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNK+FS KLASRLSNS   SH C+DC SFAT +RT NQN+ +GSFITRPV+DHS SWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSF  KIHGFFSGPV+GKRF+L  SNTL K SI+SL+    LF G+ FSKL+FQLKPGFD
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLVGRS-LFMGSPFSKLKFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            +W GRR WFQRL+TND+VLGLIIANVAVF LWRIADPIFMSKNFTISVEN RSGRIHTL
Sbjct: 121 HRWSGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+E+GHIVSNMIGLYFFGMNIGRVFGPEFLL LYLAGAIGGS FYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SS+ RPFWGKDP RAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sgr027489.1 vs. ExPASy TrEMBL
Match: A0A6J1KPT4 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495401 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.9e-156
Identity = 284/336 (84.52%), Postives = 302/336 (89.88%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNK FS KLASRLSNS   SH C+DC SFAT +RT NQN+ +GSFITRPV+DHS SWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RS   KIHGFFSGPV+GKRF+L  S+TL K SI+SL+    LF G+ FSKL+FQLKPGFD
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVERS-LFMGSPFSKLKFQLKPGFD 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            +W GRR WFQRL+TND+VLGLIIANVAVF LWRIADPIFMSKNFTISVEN RSGRIHTL
Sbjct: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSH+E+GHIVSNMIGLYFFGMNIGRVFGPEFLL LYLAGAIGGS FYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK RPFWGKDP RAQGLGASGAVNAIMLLDI LFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 335

BLAST of Sgr027489.1 vs. ExPASy TrEMBL
Match: A0A0A0LTI7 (Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.9e-153
Identity = 274/336 (81.55%), Postives = 301/336 (89.58%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNKLFS KL SRLSNS A S  CKDC SF+TL+RTHNQNHLFGSF+TRP+QDHS SWSSH
Sbjct: 1   MNKLFSRKLVSRLSNSYANSLDCKDCASFSTLVRTHNQNHLFGSFVTRPIQDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSFA KIHGFFS PV+GKRF++  SNT  K S++S++ +  LFTG  FSKL+FQLKPGF 
Sbjct: 61  RSFAQKIHGFFSVPVVGKRFIVNPSNTYFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            QW GRR WFQRL+ N++VLGLIIANVAVF LWRIADPIFMSKNFTIS+EN RSGRIHTL
Sbjct: 121 HQWGGRRSWFQRLTPNEVVLGLIIANVAVFLLWRIADPIFMSKNFTISLENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSHI+ GHIVSNM+GLYFFGMNIG VFG EFLL+LYLAGAIGGS FYLAHHLFQA 
Sbjct: 181 ITSAFSHIDTGHIVSNMVGLYFFGMNIGNVFGSEFLLKLYLAGAIGGSAFYLAHHLFQAL 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK R FWG+DP+RA+GLGASGAVNAIMLLDI LFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGEDPVRAKGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLR+LENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRMLENDSQISGSAHLGGAAVAALAWARVRRGRF 336

BLAST of Sgr027489.1 vs. ExPASy TrEMBL
Match: A0A1S3BSX2 (RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.6e-153
Identity = 277/336 (82.44%), Postives = 299/336 (88.99%), Query Frame = 0

Query: 1   MNKLFSLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFITRPVQDHSHSWSSH 60
           MNKLFS KLASRLSNS A S  CKDC SF+TL RTHNQNHLFGSF+TRP+QDHS SWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  RSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFD 120
           RSF  KIHGFFS PV+GKR ++  SNT  K S++S++ +  LFTG  FSKL+FQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 FQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIHTL 180
            QW GRR WFQRL+  D+VLGLIIANVAVF LWRIADPIFMSKNFTISVEN RSGRIHTL
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 ITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQAS 240
           ITSAFSHI+ GH++SNMIGLYFFGMNIG VFG EFLL+LYLAGAIGGS FYLAHHLFQAS
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNIGSVFGSEFLLKLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFLIGK 300
           SSK R FWGKDP+RAQGLGASGAVNAIMLLDI LFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

Query: 301 DMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           DMLRILENDSQISGSAHLGGAAVAA+AWARVRRGRF
Sbjct: 301 DMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF 336

BLAST of Sgr027489.1 vs. TAIR 10
Match: AT1G18600.1 (RHOMBOID-like protein 12 )

HSP 1 Score: 321.6 bits (823), Expect = 7.4e-88
Identity = 183/339 (53.98%), Postives = 231/339 (68.14%), Query Frame = 0

Query: 6   SLKLASRLSNSTATSHPCKDCTSFATLLRTHNQNHLFGSFI-----TRPVQ--DHSHSWS 65
           S +L   L+N T  SH  +   +F +L + +   H    ++     + PV   D S  W 
Sbjct: 14  SSRLTKLLANPTTHSHLNRQ--TFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWR 73

Query: 66  SHRSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPG 125
           S      KI GFF+  +  K            V + +L+ +   F G+QF K  F+ +  
Sbjct: 74  SE-----KIRGFFASALGNK-----------AVKLGNLVESRVGFIGSQFPKKGFEFQRF 133

Query: 126 FDFQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRIADPIFMSKNFTISVENCRSGRIH 185
             FQ RG + W Q LS  D+VLGL+IAN  VF +WR+ +  FM  NF IS++N +SGR+H
Sbjct: 134 SGFQRRGWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLH 193

Query: 186 TLITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQ 245
           TLITSAFSHI++GHIVSNMIGLYFFG +I R FGP+FLL+LYLAGA+GGSVFYL HH + 
Sbjct: 194 TLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGSVFYLIHHAYM 253

Query: 246 ASSS-KGRPFWGKDPIRAQGLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL 305
           A++S KG+  + +DP R  GLGASGAVNAIMLLDI L P++TLYLEFFIPVPA+LLGIFL
Sbjct: 254 AATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIPVPAMLLGIFL 313

Query: 306 IGKDMLRILENDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           IGKD+LRI E +S ISGSAHLGGAAVAAIAWAR+R+GRF
Sbjct: 314 IGKDILRITEGNSNISGSAHLGGAAVAAIAWARIRKGRF 334

BLAST of Sgr027489.1 vs. TAIR 10
Match: AT1G74130.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 197.2 bits (500), Expect = 2.1e-50
Identity = 116/281 (41.28%), Postives = 169/281 (60.14%), Query Frame = 0

Query: 62  SFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFDF 121
           S + ++HGFF+G +          NT  K+   +++ +   F  ++     F+      F
Sbjct: 52  SRSARVHGFFAGKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRI--ADPIFMSKNFTISVENCRSGRIHT 181
           Q RG + W      N +V GL+IAN AVF +WR+   D ++M KNF +S  +  +GRIHT
Sbjct: 112 QKRGWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIHT 171

Query: 182 LITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQA 241
           LITS FSH+   HI+ NM+GL +FG  I R FGP +LL+LY AGA+GGSVF+L+ H    
Sbjct: 172 LITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHALSV 231

Query: 242 SSSKGRPFWGKDPIRAQ--GLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL 301
            S KG+    KD ++     LGA+G V AI LLD+LL+PK T Y    + VP +  GI+ 
Sbjct: 232 ISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAGIYS 291

Query: 302 IGKDMLRILE--NDSQISGSAHLGGAAVAAIAWARVRRGRF 337
           +G +++++LE  N++ ++    LGG  VA IAWAR+R+GRF
Sbjct: 292 LGLNIIKMLEGKNNNTLTSLDQLGGVVVAVIAWARIRKGRF 320

BLAST of Sgr027489.1 vs. TAIR 10
Match: AT1G74130.2 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.1e-42
Identity = 101/250 (40.40%), Postives = 148/250 (59.20%), Query Frame = 0

Query: 62  SFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSIRSLIANMYLFTGAQFSKLRFQLKPGFDF 121
           S + ++HGFF+G +          NT  K+   +++ +   F  ++     F+      F
Sbjct: 52  SRSARVHGFFAGKL---------GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWRGRRPWFQRLSTNDMVLGLIIANVAVFFLWRI--ADPIFMSKNFTISVENCRSGRIHT 181
           Q RG + W      N +V GL+IAN AVF +WR+   D ++M KNF +S  +  +GRIHT
Sbjct: 112 QKRGWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIHT 171

Query: 182 LITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGPEFLLRLYLAGAIGGSVFYLAHHLFQA 241
           LITS FSH+   HI+ NM+GL +FG  I R FGP +LL+LY AGA+GGSVF+L+ H    
Sbjct: 172 LITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHALSV 231

Query: 242 SSSKGRPFWGKDPIRAQ--GLGASGAVNAIMLLDILLFPKSTLYLEFFIPVPAILLGIFL 301
            S KG+    KD ++     LGA+G V AI LLD+LL+PK T Y    + VP +  GI+ 
Sbjct: 232 ISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAGIYS 289

Query: 302 IGKDMLRILE 308
           +G +++++LE
Sbjct: 292 LGLNIIKMLE 289

BLAST of Sgr027489.1 vs. TAIR 10
Match: AT1G74140.5 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 166.0 bits (419), Expect = 5.2e-41
Identity = 115/310 (37.10%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 34  RTHNQNHLFGSFITRPVQDHSHSWSSHRSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSI 93
           + +   HL  S ++ P     H   S  S + K+HGFF+  +          NT  K+  
Sbjct: 31  KPNQSRHLLPSRLSSP-SSVPHFVPSAVSRSAKVHGFFASKL---------GNTNLKLKF 90

Query: 94  RSLIANMYLFTGAQFSKLRFQLKPGFDFQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLW 153
            +++ +   F  ++     F+      FQ RG + W      N +V GL+IAN AVF +W
Sbjct: 91  GNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIANAAVFTMW 150

Query: 154 RIADPIFMSKNFTISVENCRSGRIHTLITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGP 213
           R++D     +++ +S  +  SG IHTLITS FSHI    I+ NMIG+ +FG  I R  GP
Sbjct: 151 RVSD-----RSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTLGP 210

Query: 214 EFLLRLYLAGAIGGSVFYLAHHLFQASSSKGRPFWGKD-----PIRAQGLGASGAVNAIM 273
            +LL+LY AGA+GGSV +L++H   A + KG     KD     PI +Q LGA G++ AI 
Sbjct: 211 LYLLKLYFAGALGGSVCFLSYHALLA-TLKGEGVVIKDHQSTAPI-SQLLGADGSMFAIA 270

Query: 274 LLDILLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQ--ISGSAHLGGAAVAAI 333
           LLD+ ++PK T Y    + V  ++  I  +G ++L I E       S S  LGG  VAA+
Sbjct: 271 LLDMFIYPKVTTYFALMLRV-HVMFRIINLGVEILNIPEGGPNHIASSSGQLGGVVVAAM 320

Query: 334 AWARVRRGRF 337
           AWAR+++GRF
Sbjct: 331 AWARIKKGRF 320

BLAST of Sgr027489.1 vs. TAIR 10
Match: AT1G74140.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.5e-40
Identity = 115/310 (37.10%), Postives = 167/310 (53.87%), Query Frame = 0

Query: 34  RTHNQNHLFGSFITRPVQDHSHSWSSHRSFAGKIHGFFSGPVIGKRFLLTSSNTLSKVSI 93
           + +   HL  S ++ P     H   S  S + K+HGFF+  +          NT  K+  
Sbjct: 31  KPNQSRHLLPSRLSSP-SSVPHFVPSAVSRSAKVHGFFASKL---------GNTNLKLKF 90

Query: 94  RSLIANMYLFTGAQFSKLRFQLKPGFDFQWRGRRPWFQRLSTNDMVLGLIIANVAVFFLW 153
            +++ +   F  ++     F+      FQ RG + W      N +V GL+IAN AVF +W
Sbjct: 91  GNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIANAAVFTMW 150

Query: 154 RIADPIFMSKNFTISVENCRSGRIHTLITSAFSHIEVGHIVSNMIGLYFFGMNIGRVFGP 213
           R++D     +++ +S  +  SG IHTLITS FSHI    I+ NMIG+ +FG  I R  GP
Sbjct: 151 RVSD-----RSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTLGP 210

Query: 214 EFLLRLYLAGAIGGSVFYLAHHLFQASSSKGRPFWGKD-----PIRAQGLGASGAVNAIM 273
            +LL+LY AGA+GGSV +L++H   A + KG     KD     PI +Q LGA G++ AI 
Sbjct: 211 LYLLKLYFAGALGGSVCFLSYHALLA-TLKGEGVVIKDHQSTAPI-SQLLGADGSMFAIA 270

Query: 274 LLDILLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQ--ISGSAHLGGAAVAAI 333
           LLD+ ++PK T Y    + V  I+     +G ++L I E       S S  LGG  VAA+
Sbjct: 271 LLDMFIYPKVTTYFALMLRVHRII----NLGVEILNIPEGGPNHIASSSGQLGGVVVAAM 317

Query: 334 AWARVRRGRF 337
           AWAR+++GRF
Sbjct: 331 AWARIKKGRF 317