Sgr014444.1 (mRNA) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr014444.1
Type	mRNA
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Ammonium transporter
Location	tig00000589: 440053 .. 441429 (+)
Sequence length	1032
RNA-Seq Expression	Sgr014444.1
Synteny	Sgr014444.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACAACGCCGCACCGACCGGAAATATGGCTGCCGGATCTCCTCCATGGCTGAACAGCGGCGACAACGCGTGGCAGATGACCGCCTCCACTCTCGTCGGACTTCAGAGCATGCCGGGGCTGGTCATCCTCTACGCCAGCATCGTCAAGAAGAAATGGGCTGTGAACTCCGCCTTTATGGCTCTTTACGCCTTTGCCGCCGTCCTCATCTGCTGGGTTCTCGTCGGCTATCGCATGGCGTTCGGCGATCAACTTCTTCCGCTGTGGGGCAAAGGCATTCCTGCTCTCGGCCAAAATTACCTAATCAACCGAGGTAATATACCAGAGAGCGCCCATCTCCGCCATGATGGCACGGTGGAGACGCCGAGAATCGAACCGGCGGTTCCGATGGCCTCGCTCGTGTACTTCCAATTCACTTTCGCTGCGATTACTGTAATTTTGCTTGCTGGATCTGTTCTTGCCCGAATGAATATCAAGGCCTGGATGGCCTTTGTCCCTCTCTGGGTAATTTTCTCGTACACCGTCGGAGCATACACTCTCTGGGGCGGAGGGTTTCTCTTCCACTGGGGCGTCATCGATTACTCCGGCGGCTACGTCATCCATCTCTCTTCAGGAATTTCCGGCTTAACCGCCGCTTACTGGGTATTGCAAAAAAACCCAACAAATTTTACCCTACTTTGACCAAATTTCGAATCGGAATCGTTAATTGATTAATTTTGACTCTCTTTGTGGTTAGGTTGGCCCGAGAATAAAGAGCGACAGAGAGAGGTTTCCACCCAACAACGTGCTGCTGATGCTCGCCGGAGCGGGGTTGTTGTGGATGGGCTGGTCGGGATTCAACGGCGGCGCGCCCCACTCCGCCAACGTGGTGGCGTCCATTGCGGTGCTGAACACAAACGTCAGCGCCGCGACCAGCCTGCTCGTGTGGACCACTCTGGACGTCATTTTCTTCGGGAAGCCATCGGTGATCGGAGCCGTTCAGGGAATGATGACAGGCTTGGCTTGCGTTACCCCCGGCGCAGGTAACTAATATTTAACATTTATTATTCAAAGAAAGAAGATTCGTGTTTTAAATATTTATTATTTTCTTAAAATACGTGGAAAATGCTTATAAATTATGGCCTTTTTTAATTAACTCATAAATTCTGGTTGATATTATAATGTAGGGTCTTTTAGGATATTGACATTTCTATCAGATATTAATATTATTTATAAAATTTCATCGACAACGTATTTATTAATAGTAATATTTTTAACAAATTATTTAAAACAGGAGTTGTGCAGACTTGGGCGGCTATCATAATGGGGATTCTATCAGGTAGTATTCCATGGGTGTCCATGATGATCCTTCACAAAAAATTGAGTTTCTTACAAAAG

mRNA sequence

ATGAACAACGCCGCACCGACCGGAAATATGGCTGCCGGATCTCCTCCATGGCTGAACAGCGGCGACAACGCGTGGCAGATGACCGCCTCCACTCTCGTCGGACTTCAGAGCATGCCGGGGCTGGTCATCCTCTACGCCAGCATCGTCAAGAAGAAATGGGCTGTGAACTCCGCCTTTATGGCTCTTTACGCCTTTGCCGCCGTCCTCATCTGCTGGGTTCTCGTCGGCTATCGCATGGCGTTCGGCGATCAACTTCTTCCGCTGTGGGGCAAAGGCATTCCTGCTCTCGGCCAAAATTACCTAATCAACCGAGGTAATATACCAGAGAGCGCCCATCTCCGCCATGATGGCACGGTGGAGACGCCGAGAATCGAACCGGCGGTTCCGATGGCCTCGCTCGTGTACTTCCAATTCACTTTCGCTGCGATTACTGTAATTTTGCTTGCTGGATCTGTTCTTGCCCGAATGAATATCAAGGCCTGGATGGCCTTTGTCCCTCTCTGGGTAATTTTCTCGTACACCGTCGGAGCATACACTCTCTGGGGCGGAGGGTTTCTCTTCCACTGGGGCGTCATCGATTACTCCGGCGGCTACGTCATCCATCTCTCTTCAGGAATTTCCGGCTTAACCGCCGCTTACTGGGTTGGCCCGAGAATAAAGAGCGACAGAGAGAGGTTTCCACCCAACAACGTGCTGCTGATGCTCGCCGGAGCGGGGTTGTTGTGGATGGGCTGGTCGGGATTCAACGGCGGCGCGCCCCACTCCGCCAACGTGGTGGCGTCCATTGCGGTGCTGAACACAAACGTCAGCGCCGCGACCAGCCTGCTCGTGTGGACCACTCTGGACGTCATTTTCTTCGGGAAGCCATCGGTGATCGGAGCCGTTCAGGGAATGATGACAGGCTTGGCTTGCGTTACCCCCGGCGCAGGAGTTGTGCAGACTTGGGCGGCTATCATAATGGGGATTCTATCAGGTAGTATTCCATGGGTGTCCATGATGATCCTTCACAAAAAATTGAGTTTCTTACAAAAG

Coding sequence (CDS)

ATGAACAACGCCGCACCGACCGGAAATATGGCTGCCGGATCTCCTCCATGGCTGAACAGCGGCGACAACGCGTGGCAGATGACCGCCTCCACTCTCGTCGGACTTCAGAGCATGCCGGGGCTGGTCATCCTCTACGCCAGCATCGTCAAGAAGAAATGGGCTGTGAACTCCGCCTTTATGGCTCTTTACGCCTTTGCCGCCGTCCTCATCTGCTGGGTTCTCGTCGGCTATCGCATGGCGTTCGGCGATCAACTTCTTCCGCTGTGGGGCAAAGGCATTCCTGCTCTCGGCCAAAATTACCTAATCAACCGAGGTAATATACCAGAGAGCGCCCATCTCCGCCATGATGGCACGGTGGAGACGCCGAGAATCGAACCGGCGGTTCCGATGGCCTCGCTCGTGTACTTCCAATTCACTTTCGCTGCGATTACTGTAATTTTGCTTGCTGGATCTGTTCTTGCCCGAATGAATATCAAGGCCTGGATGGCCTTTGTCCCTCTCTGGGTAATTTTCTCGTACACCGTCGGAGCATACACTCTCTGGGGCGGAGGGTTTCTCTTCCACTGGGGCGTCATCGATTACTCCGGCGGCTACGTCATCCATCTCTCTTCAGGAATTTCCGGCTTAACCGCCGCTTACTGGGTTGGCCCGAGAATAAAGAGCGACAGAGAGAGGTTTCCACCCAACAACGTGCTGCTGATGCTCGCCGGAGCGGGGTTGTTGTGGATGGGCTGGTCGGGATTCAACGGCGGCGCGCCCCACTCCGCCAACGTGGTGGCGTCCATTGCGGTGCTGAACACAAACGTCAGCGCCGCGACCAGCCTGCTCGTGTGGACCACTCTGGACGTCATTTTCTTCGGGAAGCCATCGGTGATCGGAGCCGTTCAGGGAATGATGACAGGCTTGGCTTGCGTTACCCCCGGCGCAGGAGTTGTGCAGACTTGGGCGGCTATCATAATGGGGATTCTATCAGGTAGTATTCCATGGGTGTCCATGATGATCCTTCACAAAAAATTGAGTTTCTTACAAAAG

Protein sequence

MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK

Homology

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BLAST of Sgr014444.1 vs. NCBI nr
Match: XP_022971582.1 (ammonium transporter 2-like [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 8.2e-177
Identity = 313/344 (90.99%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN AP  +MA G+PPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVHMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
           ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQ+YLI+R NIPES H+ HDG V 
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQSYLIDRANIPESGHVSHDGRV- 165

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           TPRIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVG YT+
Sbjct: 166 TPRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGTYTI 225

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGG+VIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+
Sbjct: 226 WGGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGM 285

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMT
Sbjct: 286 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMT 345

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAAIIMG++ GSIPWVSMMILHKKLSFLQK
Sbjct: 346 GLACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQK 388

[Close]

BLAST of Sgr014444.1 vs. NCBI nr
Match: KAG7027883.1 (Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 628.2 bits (1619), Expect = 4.0e-176
Identity = 312/344 (90.70%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN AP  +MA G+PPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 9   MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 68

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
           ALYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R NIPES H+ HDG V 
Sbjct: 69  ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRV- 128

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           TPRIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYT+
Sbjct: 129 TPRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTI 188

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGG+VIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+
Sbjct: 189 WGGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGM 248

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMT
Sbjct: 249 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMT 308

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAAIIMG++ GSIPWVSMMILHKKLSFLQK
Sbjct: 309 GLACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQK 351

[Close]

BLAST of Sgr014444.1 vs. NCBI nr
Match: XP_023539067.1 (ammonium transporter 2 [Cucurbita pepo subsp. pepo] >KAG6596331.1 Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 628.2 bits (1619), Expect = 4.0e-176
Identity = 312/344 (90.70%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN AP  +MA G+PPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
           ALYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R NIPES H+ HDG V 
Sbjct: 61  ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRV- 120

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           TPRIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYT+
Sbjct: 121 TPRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTI 180

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGG+VIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+
Sbjct: 181 WGGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGM 240

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMT
Sbjct: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMT 300

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAAIIMG++ GSIPWVSMMILHKKLSFLQK
Sbjct: 301 GLACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQK 343

[Close]

BLAST of Sgr014444.1 vs. NCBI nr
Match: XP_022950655.1 (ammonium transporter 2-like [Cucurbita moschata])

HSP 1 Score: 627.1 bits (1616), Expect = 9.0e-176
Identity = 311/344 (90.41%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN AP  +MA G+PPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
           ALYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R NIPES H+ HDG V 
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRV- 165

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           TPRIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYT+
Sbjct: 166 TPRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTI 225

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGG+VIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+
Sbjct: 226 WGGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGM 285

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMT
Sbjct: 286 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMT 345

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAAIIMG++ GSIPWVSMMILHK+LSFLQK
Sbjct: 346 GLACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKRLSFLQK 388

[Close]

BLAST of Sgr014444.1 vs. NCBI nr
Match: XP_022934329.1 (ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 309/344 (89.83%), Postives = 328/344 (95.35%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN A  GNMAAGSPPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R NIPES +  +DG V 
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRV- 120

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           T RIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++
Sbjct: 121 TLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSV 180

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGGYVIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL
Sbjct: 181 WGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV++FGKPSVIGA+QGMMT
Sbjct: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMT 300

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAG+VQTWAA+IMGIL GSIPWVSMM+LHKKLSFLQK
Sbjct: 301 GLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQK 343

[Close]

BLAST of Sgr014444.1 vs. ExPASy Swiss-Prot
Match: Q9M6N7 (Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2)

HSP 1 Score: 518.8 bits (1335), Expect = 4.5e-146
Identity = 256/330 (77.58%), Postives = 288/330 (87.27%), Query Frame = 0


Query: 15  PPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVL 74
           P WLN GDNAWQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL+CWVL
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 75  VGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLV 134
           + Y+MAFG++LLP WGKG PA  Q YL  +  IP S               P  PMA+LV
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNS-----------NVAAPYFPMATLV 132

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVIDY 194
           YFQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IFSYTVGAY++WGGGFL+ WGVIDY
Sbjct: 133 YFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDY 192

Query: 195 SGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSG++G  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP+
Sbjct: 193 SGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPY 252

Query: 255 SANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQT 314
           +AN+ +SIAVLNTN+SAATSLLVWTTLDVIFFGKPSVIGA+QGM+TGLA VTPGAG++QT
Sbjct: 253 AANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQT 312

Query: 315 WAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           WAAII+G++SG+ PW SMMI+HKK + LQK
Sbjct: 313 WAAIIIGVVSGTAPWASMMIIHKKSALLQK 331

[Close]

BLAST of Sgr014444.1 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 2.3e-145
Identity = 245/331 (74.02%), Postives = 291/331 (87.92%), Query Frame = 0


Query: 14  SPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWV 73
           SP WLN GDNAWQMT++TLVGLQSMPGLVILY SIVKKKWA+NSAFMALYAFAAV ICWV
Sbjct: 16  SPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMALYAFAAVWICWV 75

Query: 74  LVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASL 133
           +  Y M+FGD+LLP WGK  PALGQ++L+ +  +  +A   H+G+ E P ++P  P+A++
Sbjct: 76  VWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEAPMLKPLYPVATM 135

Query: 134 VYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVID 193
           VYFQ  FA+IT+I+LAGS+L RMNIKAWMAFVPLW+ FSYTV A++LWGGGFLF WGVID
Sbjct: 136 VYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLWGGGFLFQWGVID 195

Query: 194 YSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP 253
           YSGGYVIHLSSGI+GLTAAYWVGPR  SDRERFPPNN+LL+LAGAGLLW+GW+GFNGG P
Sbjct: 196 YSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGLLWLGWTGFNGGDP 255

Query: 254 HSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQ 313
           +SAN+ +S+AVLNT++ A+TSLLVWT LDV FFGKPSVIGAVQGM+TGL C+TPGAG+VQ
Sbjct: 256 YSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMITGLVCITPGAGLVQ 315

Query: 314 TWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
            WAAI+MGILSGSIPW +MM+LHKK SF+Q+
Sbjct: 316 GWAAIVMGILSGSIPWYTMMVLHKKWSFMQR 346

[Close]

BLAST of Sgr014444.1 vs. ExPASy Swiss-Prot
Match: Q8S230 (Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-2 PE=2 SV=2)

HSP 1 Score: 513.8 bits (1322), Expect = 1.5e-144
Identity = 241/330 (73.03%), Postives = 286/330 (86.67%), Query Frame = 0


Query: 15  PPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVL 74
           P WLN GD AWQ+ A+T VG+QSMPGLV++Y SIVKKKWAVNSAFMALYA+A+ LI WVL
Sbjct: 22  PAWLNKGDTAWQLVAATFVGIQSMPGLVVIYGSIVKKKWAVNSAFMALYAYASTLIVWVL 81

Query: 75  VGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLV 134
           VG+RMAFGD+LLP W K  PAL Q++L+ R   P +AH   DGT+ETPR EP    A+LV
Sbjct: 82  VGFRMAFGDRLLPFWAKAGPALTQDFLVQRAVFPATAHYGSDGTLETPRTEPFYAEAALV 141

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVIDY 194
            F+F FAAIT++LLAGS+L RMNIKAWMAF PLW++FSYTVGA++LWGGGFL+ WGVIDY
Sbjct: 142 LFEFEFAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVGAFSLWGGGFLYQWGVIDY 201

Query: 195 SGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSG++G TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 202 SGGYVIHLSSGVAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 261

Query: 255 SANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQT 314
           + NV A++AVLNTNVSAATSLL WT LDVIFFGKPSVIGAVQGMMTGL C+TPGAG+V T
Sbjct: 262 APNVTATVAVLNTNVSAATSLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 321

Query: 315 WAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           W+A++MG+ +GS+PW +MMILHKK +FL K
Sbjct: 322 WSAMLMGMFAGSVPWFTMMILHKKSTFLMK 351

[Close]

BLAST of Sgr014444.1 vs. ExPASy Swiss-Prot
Match: Q8S233 (Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-3 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.5e-144
Identity = 244/330 (73.94%), Postives = 284/330 (86.06%), Query Frame = 0


Query: 15  PPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVL 74
           P WLN+GDN WQ+ A+T VGLQSMPGLV+LY SIVKKKWAVNSAFMALYA+A+ LI WVL
Sbjct: 18  PEWLNTGDNGWQLAAATFVGLQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASTLIVWVL 77

Query: 75  VGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLV 134
           VG+RMAFGD+LLP WGK   AL + +L+ R ++P +AH   DG +E+PR EP  P AS+V
Sbjct: 78  VGFRMAFGDRLLPFWGKAGAALTEGFLVARASVPATAHYGKDGALESPRTEPFYPEASMV 137

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVIDY 194
            FQF  AAIT++LLAGS+L RMNIKAWMAF PLW++FSYTV A++LWGGGFL+ WGVIDY
Sbjct: 138 LFQFELAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVCAFSLWGGGFLYQWGVIDY 197

Query: 195 SGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSGI+G TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 198 SGGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 257

Query: 255 SANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQT 314
           + N+ ASIAVLNTNVSAA SLL WT LDVIFFGKPSVIGAVQGMMTGL C+TPGAG+V T
Sbjct: 258 APNITASIAVLNTNVSAAASLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 317

Query: 315 WAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           WAAI+MGI  GS+PW SMMILHK+ + LQK
Sbjct: 318 WAAILMGICGGSLPWFSMMILHKRSALLQK 347

[Close]

BLAST of Sgr014444.1 vs. ExPASy Swiss-Prot
Match: Q84KJ7 (Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-1 PE=2 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 3.2e-144
Identity = 248/330 (75.15%), Postives = 286/330 (86.67%), Query Frame = 0


Query: 15  PPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVL 74
           P WLN GDNAWQ+TASTLVG+QSMPGLV+LY SIVKKKWAVNSAFMALYA+A+ L+ WVL
Sbjct: 15  PDWLNKGDNAWQLTASTLVGIQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASSLLVWVL 74

Query: 75  VGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLV 134
           VG+RMAFGDQLLP WGK   AL Q+YL+ R  +P +AH    G +  PR EP  P A+LV
Sbjct: 75  VGFRMAFGDQLLPFWGKAGVALTQSYLVGRATLPATAH----GAI--PRTEPFYPEATLV 134

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVIDY 194
            FQF FAAIT++LLAGSVL RMNIKAWMAF PLW++ SYTVGA++LWGGGFL+ WGVIDY
Sbjct: 135 LFQFEFAAITLVLLAGSVLGRMNIKAWMAFTPLWLLLSYTVGAFSLWGGGFLYRWGVIDY 194

Query: 195 SGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSGI+G TAAYWVGPR+KSDRERF PNN+LLM+AG GLLWMGW+GFNGGAP+
Sbjct: 195 SGGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWMGWAGFNGGAPY 254

Query: 255 SANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQT 314
           +AN+ AS+AVLNTNV AATSLL+WT LDVIFF KPSVIGAVQGMMTGL C+TPGAG+VQT
Sbjct: 255 AANIAASVAVLNTNVCAATSLLMWTCLDVIFFRKPSVIGAVQGMMTGLVCITPGAGLVQT 314

Query: 315 WAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           WAA++MGI +GS+PW +MMILHKK + L K
Sbjct: 315 WAAVVMGIFAGSVPWFTMMILHKKSALLMK 338

[Close]

BLAST of Sgr014444.1 vs. ExPASy TrEMBL
Match: A0A6J1I2C9 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111470255 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.9e-177
Identity = 313/344 (90.99%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN AP  +MA G+PPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVHMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
           ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQ+YLI+R NIPES H+ HDG V 
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQSYLIDRANIPESGHVSHDGRV- 165

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           TPRIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVG YT+
Sbjct: 166 TPRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGTYTI 225

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGG+VIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+
Sbjct: 226 WGGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGM 285

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMT
Sbjct: 286 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMT 345

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAAIIMG++ GSIPWVSMMILHKKLSFLQK
Sbjct: 346 GLACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQK 388

[Close]

BLAST of Sgr014444.1 vs. ExPASy TrEMBL
Match: A0A6J1GGC9 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111453662 PE=3 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.4e-176
Identity = 311/344 (90.41%), Postives = 329/344 (95.64%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN AP  +MA G+PPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
           ALYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R NIPES H+ HDG V 
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRV- 165

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           TPRIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYT+
Sbjct: 166 TPRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTI 225

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGG+VIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+
Sbjct: 226 WGGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGM 285

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMT
Sbjct: 286 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMT 345

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAAIIMG++ GSIPWVSMMILHK+LSFLQK
Sbjct: 346 GLACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKRLSFLQK 388

[Close]

BLAST of Sgr014444.1 vs. ExPASy TrEMBL
Match: A0A6J1F297 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 7.0e-174
Identity = 309/344 (89.83%), Postives = 328/344 (95.35%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN A  GNMAAGSPPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R NIPES +  +DG V 
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRV- 120

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           T RIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++
Sbjct: 121 TLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSV 180

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGGYVIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL
Sbjct: 181 WGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV++FGKPSVIGA+QGMMT
Sbjct: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMT 300

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAG+VQTWAA+IMGIL GSIPWVSMM+LHKKLSFLQK
Sbjct: 301 GLACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQK 343

[Close]

BLAST of Sgr014444.1 vs. ExPASy TrEMBL
Match: A0A6J1IZU0 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.0e-173
Identity = 309/344 (89.83%), Postives = 328/344 (95.35%), Query Frame = 0


Query: 1   MNNAAPTGNMAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNN A  GNMAAGSPPWLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVE 120
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL Q+YLI+R +IPES +  +DG V 
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRV- 120

Query: 121 TPRIEPAVPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTL 180
           T RIEP +PMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++
Sbjct: 121 TLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSV 180

Query: 181 WGGGFLFHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240
           WGGGFLF WGVIDYSGGYVIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL
Sbjct: 181 WGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGL 240

Query: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMT 300
           LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWT LDV++FGKPSVIGA+QGMMT
Sbjct: 241 LWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMT 300

Query: 301 GLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GLACVTPGAGVVQTWAA+IMGIL GSIPWVSMM+LHKKLSFLQK
Sbjct: 301 GLACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQK 343

[Close]

BLAST of Sgr014444.1 vs. ExPASy TrEMBL
Match: A0A0A0LCQ1 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.2e-168
Identity = 303/335 (90.45%), Postives = 320/335 (95.52%), Query Frame = 0


Query: 10  MAAGSPPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 69
           MAAGSP WLN+GDN+WQ+TASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 70  ICWVLVGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVP 129
           ICWV+VGYRMAFGDQL+PLWGKGIPAL Q+YLI +GNIPES    +DG   TPRI+P VP
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESG--VNDGR-GTPRIQPNVP 120

Query: 130 MASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHW 189
           MASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIFSYTVGAY++WGGGFLF W
Sbjct: 121 MASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQW 180

Query: 190 GVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 249
           GVIDYSGGYVIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN
Sbjct: 181 GVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 240

Query: 250 GGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGA 309
           GGAPHSANVVASIAVLNTNVSAATSLLVWT LDV +FGKPSVIGA+QGMMTGLACVTPGA
Sbjct: 241 GGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGA 300

Query: 310 GVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           GVVQTWAAIIMGIL+GSIPW+SMM+LHKKLSFLQK
Sbjct: 301 GVVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQK 332

[Close]

BLAST of Sgr014444.1 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 518.8 bits (1335), Expect = 3.2e-147
Identity = 256/330 (77.58%), Postives = 288/330 (87.27%), Query Frame = 0


Query: 15  PPWLNSGDNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVL 74
           P WLN GDNAWQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL+CWVL
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 75  VGYRMAFGDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLV 134
           + Y+MAFG++LLP WGKG PA  Q YL  +  IP S               P  PMA+LV
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNS-----------NVAAPYFPMATLV 132

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHWGVIDY 194
           YFQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IFSYTVGAY++WGGGFL+ WGVIDY
Sbjct: 133 YFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDY 192

Query: 195 SGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSG++G  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP+
Sbjct: 193 SGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPY 252

Query: 255 SANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGAGVVQT 314
           +AN+ +SIAVLNTN+SAATSLLVWTTLDVIFFGKPSVIGA+QGM+TGLA VTPGAG++QT
Sbjct: 253 AANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQT 312

Query: 315 WAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           WAAII+G++SG+ PW SMMI+HKK + LQK
Sbjct: 313 WAAIIIGVVSGTAPWASMMIIHKKSALLQK 331

[Close]

BLAST of Sgr014444.1 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 372.5 bits (955), Expect = 3.7e-103
Identity = 179/215 (83.26%), Postives = 203/215 (94.42%), Query Frame = 0


Query: 130 MASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWGGGFLFHW 189
           MA+LVYFQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IFSYTVGAY++WGGGFL+ W
Sbjct: 1   MATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQW 60

Query: 190 GVIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 249
           GVIDYSGGYVIHLSSG++G  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFN
Sbjct: 61  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 120

Query: 250 GGAPHSANVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSVIGAVQGMMTGLACVTPGA 309
           GGAP++AN+ +SIAVLNTN+SAATSLLVWTTLDVIFFGKPSVIGA+QGM+TGLA VTPGA
Sbjct: 121 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 180

Query: 310 GVVQTWAAIIMGILSGSIPWVSMMILHKKLSFLQK 345
           G++QTWAAII+G++SG+ PW SMMI+HKK + LQK
Sbjct: 181 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQK 215

[Close]

BLAST of Sgr014444.1 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 79.3 bits (194), Expect = 6.5e-15
Identity = 90/338 (26.63%), Postives = 133/338 (39.35%), Query Frame = 0


Query: 22  DNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVLVGYRMAF 81
           DN + + ++ LV    + G  +L A  V+ K  +N     +   AA  + + L GY  AF
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 82  GDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLVY----FQ 141
           G       GK    L                               +P AS  Y    +Q
Sbjct: 102 GSPSNGFIGKHYFGLKD-----------------------------IPTASADYSNFLYQ 161

Query: 142 FTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWG-----------GGFL 201
           + FA     + +GS+  R    A++ +      F Y V ++  W            G  L
Sbjct: 162 WAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLL 221

Query: 202 FHWGVIDYSGGYVIHLSSGISGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLL 261
           F  G ID++G  V+H+  GI+GL  A   GPR+ + D          ++  L++ G  LL
Sbjct: 222 FSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLL 281

Query: 262 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTTLD------VIFFGKP------ 321
           W GW GFN G+ +   V       N   SA     V TTL          FGK       
Sbjct: 282 WFGWYGFNPGSFNKILVTYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHW 341

Query: 322 SVIGAVQGMMTGLACVTPGAGVVQTWAAIIMGILSGSI 328
           +V     G++ G A +T G  VV+ WAAII G ++  +
Sbjct: 342 NVTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALV 349

[Close]

BLAST of Sgr014444.1 vs. TAIR 10
Match: AT1G64780.1 (ammonium transporter 1;2 )

HSP 1 Score: 78.6 bits (192), Expect = 1.1e-14
Identity = 91/350 (26.00%), Postives = 141/350 (40.29%), Query Frame = 0


Query: 22  DNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVLVGYRMAF 81
           DN + + ++ LV    + G  +L A  V+ K  +N     +   AA  I + L G+  AF
Sbjct: 52  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAF 111

Query: 82  GDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLVYFQFTFA 141
           G                N  I R          H     +   E      S   +Q+ FA
Sbjct: 112 GTP-------------SNGFIGR---------HHSFFALSSYPERPGSDFSFFLYQWAFA 171

Query: 142 AITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLWG-----------GGFLFHWG 201
                + +GS+  R    A++ +      F Y   ++  W               LF  G
Sbjct: 172 IAAAGITSGSIAERTQFVAYLIYSTFLTGFVYPTVSHWFWSSDGWASASRSDNNLLFGSG 231

Query: 202 VIDYSGGYVIHLSSGISGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLWMGW 261
            ID++G  V+H+  GI+GL  A   GPRI + DR         ++  L++ G  LLW GW
Sbjct: 232 AIDFAGSGVVHMVGGIAGLCGALVEGPRIGRFDRSGRSVALRGHSASLVVLGTFLLWFGW 291

Query: 262 SGFNGGA------------PHSA--NVVASIAVLNTNVSAATSLLVWTTLDVIFFGKPSV 321
            GFN G+            P+    + V   AV  T +S  T+ L       +  G  +V
Sbjct: 292 YGFNPGSFLTILKGYDKSRPYYGQWSAVGRTAV-TTTLSGCTAALTTLFSKRLLAGHWNV 351

Query: 322 IGAVQGMMTGLACVTPGAGVVQTWAAIIMGILSGSIPWVSMMILHKKLSF 342
           I    G++ G A +T G  VV+ WAAI+ G ++  +  +   +L KKL +
Sbjct: 352 IDVCNGLLGGFAAITSGCAVVEPWAAIVCGFVASWV-LIGFNLLAKKLKY 376

[Close]

BLAST of Sgr014444.1 vs. TAIR 10
Match: AT3G24300.1 (ammonium transporter 1;3 )

HSP 1 Score: 75.5 bits (184), Expect = 9.4e-14
Identity = 85/339 (25.07%), Postives = 138/339 (40.71%), Query Frame = 0


Query: 22  DNAWQMTASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLICWVLVGYRMAF 81
           DN + + ++ LV    + G  +L A  V+ K  +N     +   AA  + + L GY  AF
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 82  GDQLLPLWGKGIPALGQNYLINRGNIPESAHLRHDGTVETPRIEPAVPMASLVYFQFTFA 141
           G       G+       N+ +     P + +                   S   +Q+ FA
Sbjct: 106 GGSSEGFIGR------HNFALRDFPTPTADY-------------------SFFLYQWAFA 165

Query: 142 AITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTLW-----------GGGFLFHWG 201
                + +GS+  R    A++ +      F Y V ++  W               LF  G
Sbjct: 166 IAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTG 225

Query: 202 VIDYSGGYVIHLSSGISGLTAAYWVGPRIKSDRERFPP---------NNVLLMLAGAGLL 261
            ID++G  V+H+  GI+GL  A   GPR    R RF           ++  L++ G  LL
Sbjct: 226 AIDFAGSGVVHMVGGIAGLWGALIEGPR----RGRFEKGGRAIALRGHSASLVVLGTFLL 285

Query: 262 WMGWSGFNGGA------PHSA-------NVVASIAVLNTNVSAATSLLVWTTLDVIFFGK 321
           W GW GFN G+      P+++       + +   AV NT +S  T+ L       +  G 
Sbjct: 286 WFGWYGFNPGSFTKILVPYNSGSNYGQWSGIGRTAV-NTTLSGCTAALTTLFGKRLLSGH 345

Query: 322 PSVIGAVQGMMTGLACVTPGAGVVQTWAAIIMGILSGSI 328
            +V     G++ G A +T G  VV+ WAAI+ G ++  +
Sbjct: 346 WNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFMASVV 353

The following BLAST results are available for this feature:

BLAST of Sgr014444.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022971582.1	8.2e-177	90.99	ammonium transporter 2-like [Cucurbita maxima]	[more]
KAG7027883.1	4.0e-176	90.70	Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023539067.1	4.0e-176	90.70	ammonium transporter 2 [Cucurbita pepo subsp. pepo] >KAG6596331.1 Ammonium trans...	[more]
XP_022950655.1	9.0e-176	90.41	ammonium transporter 2-like [Cucurbita moschata]	[more]
XP_022934329.1	1.4e-173	89.83	ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transpor...	[more]

BLAST of Sgr014444.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M6N7	4.5e-146	77.58	Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2	[more]
Q69T29	2.3e-145	74.02	Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3...	[more]
Q8S230	1.5e-144	73.03	Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]
Q8S233	2.5e-144	73.94	Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]
Q84KJ7	3.2e-144	75.15	Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2...	[more]

BLAST of Sgr014444.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I2C9	3.9e-177	90.99	Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111470255 PE=3 SV=1	[more]
A0A6J1GGC9	4.4e-176	90.41	Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111453662 PE=3 SV=1	[more]
A0A6J1F297	7.0e-174	89.83	Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1	[more]
A0A6J1IZU0	2.0e-173	89.83	Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1	[more]
A0A0A0LCQ1	1.2e-168	90.45	Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1	[more]

BLAST of Sgr014444.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38290.1	3.2e-147	77.58	ammonium transporter 2	[more]
AT2G38290.2	3.7e-103	83.26	ammonium transporter 2	[more]
AT4G13510.1	6.5e-15	26.63	ammonium transporter 1;1	[more]
AT1G64780.1	1.1e-14	26.00	ammonium transporter 1;2	[more]
AT3G24300.1	9.4e-14	25.07	ammonium transporter 1;3	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002229	Blood group Rhesus C/E/D polypeptide	PRINTS	PR00342	RHESUSRHD	coord: 263..285 score: 24.88 coord: 137..153 score: 33.99 coord: 232..250 score: 41.52 coord: 318..336 score: 19.01
IPR024041	Ammonium transporter AmtB-like domain	PFAM	PF00909	Ammonium_transp	coord: 24..339 e-value: 3.8E-58 score: 197.1
IPR029020	Ammonium/urea transporter	GENE3D	1.10.3430.10	Ammonium transporter AmtB like domains	coord: 2..341 e-value: 2.2E-88 score: 298.9
None	No IPR available	PANTHER	PTHR43029:SF34	AMMONIUM TRANSPORTER 2	coord: 9..344
None	No IPR available	PANTHER	PTHR43029	AMMONIUM TRANSPORTER MEP2	coord: 9..344
None	No IPR available	SUPERFAMILY	111352	Ammonium transporter	coord: 17..339
IPR018047	Ammonium transporter, conserved site	PROSITE	PS01219	AMMONIUM_TRANSP	coord: 193..218

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sgr014444	Sgr014444	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sgr014444.1.exon1	Sgr014444.1.exon1	exon
Sgr014444.1.exon2	Sgr014444.1.exon2	exon
Sgr014444.1.exon3	Sgr014444.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Sgr014444.1	cds.Sgr014444.1	CDS
cds.Sgr014444.1	cds.Sgr014444.1_2	CDS
cds.Sgr014444.1	cds.Sgr014444.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sgr014444.1	Sgr014444.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0072488	ammonium transmembrane transport
biological_process	GO:0015696	ammonium transport
biological_process	GO:0019740	nitrogen utilization
cellular_component	GO:0005887	integral component of plasma membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0008519	ammonium transmembrane transporter activity
molecular_function	GO:0015398	high-affinity secondary active ammonium transmembrane transporter activity