Sed0009661.1 (mRNA) Chayote v1

Overview


Name	Sed0009661.1
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	aspartic proteinase PCS1-like
Location	LG05: 38712791 .. 38714286 (+)
Sequence length	1496
RNA-Seq Expression	Sed0009661.1
Synteny	Sed0009661.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCTCTTTCCTCTATATATACAAAACCCAACATCCCCATTTCACCAAAAACCCCACAACTAAAAATGATTTCCTTTCTCTTCTCTCTCTCCCTTTTTTCTCTCTTCTTCTCCCACTCCAATTCCCTCTCTCTCTCCTTCCCCCTTTCTCTCTCTAAAACCACTTCTCCAAAATCCCCATTATACCCTTTCTTATACTCCAAAACGACGTCGCATGACTCCGCCAAGCTGCTCTTCAAATACTCCACCGCCCTCGTCGTGTCTCTCCCCATCGGAACGCCGCCGCAGCCGACGGACTTCGTGCTCGACACCGGCAGCCAGCTGTCGTGGATTCAGTGCCACGACAGAGTCAAGCCCAAAACGACGTCGTTCGACCCTTCTCTCTCCTCCTCTTTCTCTCTCCTCCCCTGCAACAACCCTCTCTGCAAGCCCCGAATTCCCGATTTTACCCTCCCCACTTCCTGTGACCAAAATCGCCTCTGCCACTACTCGTACTTCTACGCGGACGGCACCTTGGCCGAGGGCAATCTCGTCCGAGAAAAATTCGCCCTCTCAAATTACCTCACTACCCCTCCCGTCATCCTCGGCTGCACCAAAGCCTCCTCCGAAAACAGGGGCATTTTGGGGATGAACGCCGGCCGCCTCTCCTTCATCTCCCAAGCCAGAATCAACAAGTTTTCCTATTGCATTCCCGGTTCAACCCGACCCGGCTCCACCGGGCTCTTCTACCTCGGACAAAACCCGAACTCCGCCCACTTCAGATACGTCCCCATGTTGACCCTCCCCAAAAGTCAACGCTCCCCCAATCTCGACCCGCTTGCCTACACCCTCCCGATGAAGGGGATAAAAATAGGCGGGAGCCGGCTCAATATATCTTCGGCCGTGTTTAAACCGGACCGGACCGGGTCCGGTCAGACCATGGTGGACTCCGGTTCGGACTTGACGTATCTCGTGGACGAAGCGTACGATAAAGTTAGAGAGGCGATGGTGAGGTTAGTGGGGCCCATGATGAAGAAGGGGTACGAATACGCCGCCGTGGCCGACATGTGTTTCGACGGGTGGACGGCGGCGGCTGCGGCGGGGCGGAGGATTGGCGACATGTCGTTCGAGTTTGAAAACGGGGTGGAGGTTTTGGTGGGGAAAGGGGAAGGGGTATTAACGGAAGTTGAAAAGGGGGTGAAGTGCGTGGCGATCGGACGGTCGGGGAGGCTTGGGATTGGGAGTAATATAATCGGGAACGTTCATCAGCGGAATATGTGGGTGGAGTATGACTTGGCCAATAGGAGAATTGGGTTTGGTGGAGCTGTGTGTAGTGGATTGAAGTGATGATGGACGGTGGCGATTAATACACGTGTTCAGGCTTGTGGAGGGTTCTAGAAAAGGAGGGGTTTTGAATGTATCAAGAATGGCACTATTGGAAAACTATGTGTATATATGTGTATACTTTTATACTTCATTTATATAATAAAGTGAATTTTATTCTATTATGTGAAAAA

mRNA sequence

CTTTCTCTTTCCTCTATATATACAAAACCCAACATCCCCATTTCACCAAAAACCCCACAACTAAAAATGATTTCCTTTCTCTTCTCTCTCTCCCTTTTTTCTCTCTTCTTCTCCCACTCCAATTCCCTCTCTCTCTCCTTCCCCCTTTCTCTCTCTAAAACCACTTCTCCAAAATCCCCATTATACCCTTTCTTATACTCCAAAACGACGTCGCATGACTCCGCCAAGCTGCTCTTCAAATACTCCACCGCCCTCGTCGTGTCTCTCCCCATCGGAACGCCGCCGCAGCCGACGGACTTCGTGCTCGACACCGGCAGCCAGCTGTCGTGGATTCAGTGCCACGACAGAGTCAAGCCCAAAACGACGTCGTTCGACCCTTCTCTCTCCTCCTCTTTCTCTCTCCTCCCCTGCAACAACCCTCTCTGCAAGCCCCGAATTCCCGATTTTACCCTCCCCACTTCCTGTGACCAAAATCGCCTCTGCCACTACTCGTACTTCTACGCGGACGGCACCTTGGCCGAGGGCAATCTCGTCCGAGAAAAATTCGCCCTCTCAAATTACCTCACTACCCCTCCCGTCATCCTCGGCTGCACCAAAGCCTCCTCCGAAAACAGGGGCATTTTGGGGATGAACGCCGGCCGCCTCTCCTTCATCTCCCAAGCCAGAATCAACAAGTTTTCCTATTGCATTCCCGGTTCAACCCGACCCGGCTCCACCGGGCTCTTCTACCTCGGACAAAACCCGAACTCCGCCCACTTCAGATACGTCCCCATGTTGACCCTCCCCAAAAGTCAACGCTCCCCCAATCTCGACCCGCTTGCCTACACCCTCCCGATGAAGGGGATAAAAATAGGCGGGAGCCGGCTCAATATATCTTCGGCCGTGTTTAAACCGGACCGGACCGGGTCCGGTCAGACCATGGTGGACTCCGGTTCGGACTTGACGTATCTCGTGGACGAAGCGTACGATAAAGTTAGAGAGGCGATGGTGAGGTTAGTGGGGCCCATGATGAAGAAGGGGTACGAATACGCCGCCGTGGCCGACATGTGTTTCGACGGGTGGACGGCGGCGGCTGCGGCGGGGCGGAGGATTGGCGACATGTCGTTCGAGTTTGAAAACGGGGTGGAGGTTTTGGTGGGGAAAGGGGAAGGGGTATTAACGGAAGTTGAAAAGGGGGTGAAGTGCGTGGCGATCGGACGGTCGGGGAGGCTTGGGATTGGGAGTAATATAATCGGGAACGTTCATCAGCGGAATATGTGGGTGGAGTATGACTTGGCCAATAGGAGAATTGGGTTTGGTGGAGCTGTGTGTAGTGGATTGAAGTGATGATGGACGGTGGCGATTAATACACGTGTTCAGGCTTGTGGAGGGTTCTAGAAAAGGAGGGGTTTTGAATGTATCAAGAATGGCACTATTGGAAAACTATGTGTATATATGTGTATACTTTTATACTTCATTTATATAATAAAGTGAATTTTATTCTATTATGTGAAAAA

Coding sequence (CDS)

ATGATTTCCTTTCTCTTCTCTCTCTCCCTTTTTTCTCTCTTCTTCTCCCACTCCAATTCCCTCTCTCTCTCCTTCCCCCTTTCTCTCTCTAAAACCACTTCTCCAAAATCCCCATTATACCCTTTCTTATACTCCAAAACGACGTCGCATGACTCCGCCAAGCTGCTCTTCAAATACTCCACCGCCCTCGTCGTGTCTCTCCCCATCGGAACGCCGCCGCAGCCGACGGACTTCGTGCTCGACACCGGCAGCCAGCTGTCGTGGATTCAGTGCCACGACAGAGTCAAGCCCAAAACGACGTCGTTCGACCCTTCTCTCTCCTCCTCTTTCTCTCTCCTCCCCTGCAACAACCCTCTCTGCAAGCCCCGAATTCCCGATTTTACCCTCCCCACTTCCTGTGACCAAAATCGCCTCTGCCACTACTCGTACTTCTACGCGGACGGCACCTTGGCCGAGGGCAATCTCGTCCGAGAAAAATTCGCCCTCTCAAATTACCTCACTACCCCTCCCGTCATCCTCGGCTGCACCAAAGCCTCCTCCGAAAACAGGGGCATTTTGGGGATGAACGCCGGCCGCCTCTCCTTCATCTCCCAAGCCAGAATCAACAAGTTTTCCTATTGCATTCCCGGTTCAACCCGACCCGGCTCCACCGGGCTCTTCTACCTCGGACAAAACCCGAACTCCGCCCACTTCAGATACGTCCCCATGTTGACCCTCCCCAAAAGTCAACGCTCCCCCAATCTCGACCCGCTTGCCTACACCCTCCCGATGAAGGGGATAAAAATAGGCGGGAGCCGGCTCAATATATCTTCGGCCGTGTTTAAACCGGACCGGACCGGGTCCGGTCAGACCATGGTGGACTCCGGTTCGGACTTGACGTATCTCGTGGACGAAGCGTACGATAAAGTTAGAGAGGCGATGGTGAGGTTAGTGGGGCCCATGATGAAGAAGGGGTACGAATACGCCGCCGTGGCCGACATGTGTTTCGACGGGTGGACGGCGGCGGCTGCGGCGGGGCGGAGGATTGGCGACATGTCGTTCGAGTTTGAAAACGGGGTGGAGGTTTTGGTGGGGAAAGGGGAAGGGGTATTAACGGAAGTTGAAAAGGGGGTGAAGTGCGTGGCGATCGGACGGTCGGGGAGGCTTGGGATTGGGAGTAATATAATCGGGAACGTTCATCAGCGGAATATGTGGGTGGAGTATGACTTGGCCAATAGGAGAATTGGGTTTGGTGGAGCTGTGTGTAGTGGATTGAAGTGA

Protein sequence

MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYSTALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRVKPKTTSFDPSLSSSFSLLPCNNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGCTKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAVCSGLK

Homology

[Close]

BLAST of Sed0009661.1 vs. NCBI nr
Match: XP_038878004.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 688.7 bits (1776), Expect = 3.1e-194
Identity = 347/424 (81.84%), Postives = 371/424 (87.50%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYS 60
           ++   FSLSLFSL FS SNSLSLSFPLSLSK TS  SPLYP LYSKT+SH S KL  KYS
Sbjct: 2   LLILFFSLSLFSLSFSQSNSLSLSFPLSLSKKTSNISPLYPSLYSKTSSHGSFKLPVKYS 61

Query: 61  TALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRV-----KPKTTSFDPSLSSSFSLLPC 120
           TALVVSLPIGTPPQPTD VLDTGSQLSWIQCHD+V     KPKT SFDPSLSSSFSLLPC
Sbjct: 62  TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPKPKTASFDPSLSSSFSLLPC 121

Query: 121 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 180
           N+P+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN LTTPPVILGC
Sbjct: 122 NHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSLTTPPVILGC 181

Query: 181 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVP 240
            +AS+ENRGILGMN GRLSFISQA+I+KFSYC+P  TR   TGLFYLG NPNS+ F+YV 
Sbjct: 182 AQASTENRGILGMNTGRLSFISQAKISKFSYCVPRRTRFNPTGLFYLGDNPNSSKFKYVT 241

Query: 241 MLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYL 300
           MLT PKSQRSPNLDPLAYTLPMKGIKI G RLNIS A FKPD +GSGQTM+DSGSDLTYL
Sbjct: 242 MLTFPKSQRSPNLDPLAYTLPMKGIKIAGKRLNISPAFFKPDASGSGQTMIDSGSDLTYL 301

Query: 301 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENGVEV 360
            DEAY+KV+E +VRLVGPMMKKGYE+AAVADMCF+    AA  GRRIG+MSFEFENGVE+
Sbjct: 302 ADEAYEKVKEEIVRLVGPMMKKGYEFAAVADMCFNA-GEAAVVGRRIGNMSFEFENGVEI 361

Query: 361 LVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAVC 420
           LVGKGEGVL EVEKGVKCV IGRSGRLGIGSNIIG VHQ+NMWVEYDLA +RIGFGGA C
Sbjct: 362 LVGKGEGVLAEVEKGVKCVGIGRSGRLGIGSNIIGTVHQQNMWVEYDLAKKRIGFGGAEC 421

[Close]

BLAST of Sed0009661.1 vs. NCBI nr
Match: XP_022934878.1 (aspartic proteinase PCS1-like [Cucurbita moschata])

HSP 1 Score: 674.9 bits (1740), Expect = 4.6e-190
Identity = 341/425 (80.24%), Postives = 371/425 (87.29%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYS 60
           M+  LF LSL SL FSHS+SLSLSFPLSLSK  SP S     L+S  +++ S KL FKYS
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPSPVSSSVSPLFSSLSAYGSVKLPFKYS 60

Query: 61  TALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRV-----KPKTTSFDPSLSSSFSLLPC 120
           TALVVSLPIGTPPQPTD VLDTGSQLSWIQCH +V     KPKTTSFDPSLSSSFSLLPC
Sbjct: 61  TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKKLLKPKTTSFDPSLSSSFSLLPC 120

Query: 121 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 180
           N+PLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN  TTPPVILGC
Sbjct: 121 NHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTPPVILGC 180

Query: 181 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVP 240
            +AS+ENRGILGMN GRLSF+SQA+I+KFSYC+PG T P STGLFYLG NPNSA+F+Y+ 
Sbjct: 181 AQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSANFKYIS 240

Query: 241 MLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYL 300
           +LT PKSQRSPNLDPLAYTLP+KGIKI G+RLNISSAVFKPDR+GSGQTM+DSGSDLTYL
Sbjct: 241 LLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSGSDLTYL 300

Query: 301 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF-DGWTAAAAAGRRIGDMSFEFENGVE 360
           VDEAY+KV+E +V+LVGP+MKKGYEYAAVADMCF DG T  A  GRRI DMSFEFENGVE
Sbjct: 301 VDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGET--AEVGRRIRDMSFEFENGVE 360

Query: 361 VLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAV 420
           + VGKGEGVLTEVEKGVKCV  GRSGRLGI SNIIG VHQ+N W+EYDLANRRIGFGGA 
Sbjct: 361 ISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLANRRIGFGGAD 420

[Close]

BLAST of Sed0009661.1 vs. NCBI nr
Match: KAG6581233.1 (Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 673.3 bits (1736), Expect = 1.3e-189
Identity = 341/425 (80.24%), Postives = 370/425 (87.06%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYS 60
           M+  LF LSL SL FSHS+SLSLSFPLSLSK  SP S     L+S  +++ S KL FKYS
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPSPVSSSVSPLFSSLSAYGSVKLPFKYS 60

Query: 61  TALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRV-----KPKTTSFDPSLSSSFSLLPC 120
           TALVVSLPIGTPPQPTD VLDTGSQLSWIQCH +V     KPKT SFDPSLSSSFSLLPC
Sbjct: 61  TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKKLLKPKTASFDPSLSSSFSLLPC 120

Query: 121 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 180
           N+PLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN  TTPPVILGC
Sbjct: 121 NHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTPPVILGC 180

Query: 181 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVP 240
            +AS+ENRGILGMN GRLSF+SQA+I+KFSYC+PG T P STGLFYLG NPNSA+F+Y+ 
Sbjct: 181 AQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSANFKYIS 240

Query: 241 MLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYL 300
           +LT PKSQRSPNLDPLAYTLP+KGIKI G+RLNISSAVFKPDR+GSGQTM+DSGSDLTYL
Sbjct: 241 LLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSGSDLTYL 300

Query: 301 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF-DGWTAAAAAGRRIGDMSFEFENGVE 360
           VDEAY+KV+E +V+LVGP+MKKGYEYAAVADMCF DG T  A  GRRI DMSFEFENGVE
Sbjct: 301 VDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGET--AEVGRRIRDMSFEFENGVE 360

Query: 361 VLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAV 420
           + VGKGEGVLTEVEKGVKCV  GRSGRLGI SNIIG VHQ+N WVEYDLANRRIGFGGA 
Sbjct: 361 ISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIGFGGAD 420

[Close]

BLAST of Sed0009661.1 vs. NCBI nr
Match: XP_022983616.1 (aspartic proteinase PCS1-like [Cucurbita maxima])

HSP 1 Score: 671.8 bits (1732), Expect = 3.9e-189
Identity = 340/425 (80.00%), Postives = 370/425 (87.06%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYS 60
           M+  LF +SL SL FSHS+SL LSFPLSLSK  SP SP    L+S  +S+ S KL FKYS
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSP----LFSSLSSYGSVKLPFKYS 60

Query: 61  TALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDR-----VKPKTTSFDPSLSSSFSLLPC 120
           TALVVSLPIGTPPQPTD VLDTGSQLSWIQCH +     +KPKT SFDPSLSSSFSLLPC
Sbjct: 61  TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKKLLKPKTASFDPSLSSSFSLLPC 120

Query: 121 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 180
           N+PLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN  TTPPVILGC
Sbjct: 121 NHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTPPVILGC 180

Query: 181 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVP 240
            +AS+ENRGILGMN GRLSF+SQA+I+KFSYC+PG T P STGLFYLG NPNSA+F+Y+ 
Sbjct: 181 AQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSANFKYIS 240

Query: 241 MLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYL 300
           +LT PKSQRSPNLDPLAYTLP+KGIKI G+RLNISSAVFKPDR+GSGQTM+DSGSDLTYL
Sbjct: 241 LLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSGSDLTYL 300

Query: 301 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF-DGWTAAAAAGRRIGDMSFEFENGVE 360
           VDEAY+KV+E +V+LVGP+MKKGYEYAAVADMCF DG T  A  GRRI DMSFEFENGVE
Sbjct: 301 VDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGET--AEVGRRIRDMSFEFENGVE 360

Query: 361 VLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAV 420
           + VGKGEGVLTEVEKGVKCV  GRSGRLGI SNIIG VHQ+N WVEYDLANRRIGFGGA 
Sbjct: 361 ISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIGFGGAD 419

[Close]

BLAST of Sed0009661.1 vs. NCBI nr
Match: XP_023526382.1 (aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-188
Identity = 339/426 (79.58%), Postives = 370/426 (86.85%), Query Frame = 0


Query: 4   FLFSLSLFSLFFSHSNSLSLSFPLSLSK----TTSPKSPLYPFLYSKTTSHDSAKLLFKY 63
           +L  LSL SL FSHS+SLSLSFPLS+SK     +SP SP    L+S  +S+ S KL FKY
Sbjct: 7   YLSLLSLLSLSFSHSHSLSLSFPLSVSKRPSPVSSPVSPPVSPLFSSLSSYGSVKLPFKY 66

Query: 64  STALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDR-----VKPKTTSFDPSLSSSFSLLP 123
           STALVVSLPIGTPPQPTD VLDTGSQLSWIQCH +     +KPKT SFDPSLSSSFSLLP
Sbjct: 67  STALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKKLLKPKTASFDPSLSSSFSLLP 126

Query: 124 CNNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILG 183
           CN+PLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN  TTPPVILG
Sbjct: 127 CNHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTPPVILG 186

Query: 184 CTKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYV 243
           C +AS+ENRGILGMN GRLSF+SQA+I+KFSYC+PG T P STGLFYLG NPNSAHF+Y+
Sbjct: 187 CAQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSAHFKYI 246

Query: 244 PMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTY 303
            +LT PKSQRSPNLDPLAYTLP+KGIKI G+RLNISSAVFKPDR+GSGQTM+DSGSDLTY
Sbjct: 247 SLLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSGSDLTY 306

Query: 304 LVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF-DGWTAAAAAGRRIGDMSFEFENGV 363
           LVDEAY+KV+E +V+LVGP+MKKGYEYAAVADMCF DG T  A  GRRI DMSFEFENGV
Sbjct: 307 LVDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGET--AEVGRRIRDMSFEFENGV 366

Query: 364 EVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGA 420
           E+ VGKGEGVLTEVEKGVKCV  GRSGRLGI SNIIG VHQ+N W+EYDLANRRIGFGGA
Sbjct: 367 EISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLANRRIGFGGA 426

[Close]

BLAST of Sed0009661.1 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.0e-63
Identity = 152/426 (35.68%), Postives = 222/426 (52.11%), Query Frame = 0


Query: 16  SHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHD---SAKLLFKYSTALVVSLPIGTP 75
           S S+S S SF  S   ++S    L   L ++ T  D   + KL F ++  L V+L +GTP
Sbjct: 23  SSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTP 82

Query: 76  PQPTDFVLDTGSQLSWIQCHDRVKPK-TTSFDPSLSSSFSLLPCNNPLCKPRIPDFTLPT 135
           PQ    V+DTGS+LSW++C+    P    +FDP+ SSS+S +PC++P C+ R  DF +P 
Sbjct: 83  PQNISMVIDTGSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPA 142

Query: 136 SCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC--------TKASSENR 195
           SCD ++LCH +  YAD + +EGNL  E F   N      +I GC         +  ++  
Sbjct: 143 SCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTT 202

Query: 196 GILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNS--AHFRYVPMLTLPK 255
           G+LGMN G LSFISQ    KFSYCI G+      G   LG +  +      Y P++ +  
Sbjct: 203 GLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGDSNFTWLTPLNYTPLIRI-- 262

Query: 256 SQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYLVDEAYD 315
           S   P  D +AYT+ + GIK+ G  L I  +V  PD TG+GQTMVDSG+  T+L+   Y 
Sbjct: 263 STPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYT 322

Query: 316 KVREAMVRLVGPMM----KKGYEYAAVADMCFDGWTAAAAAG--RRIGDMSFEFENGVEV 375
            +R   +     ++       + +    D+C+        +G   R+  +S  FE G E+
Sbjct: 323 ALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFE-GAEI 382

Query: 376 LVGKGEGVLTEV------EKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIG 416
            V  G+ +L  V         V C   G S  +G+ + +IG+ HQ+NMW+E+DL   RIG
Sbjct: 383 AV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIG 442

[Close]

BLAST of Sed0009661.1 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.1e-40
Identity = 113/363 (31.13%), Postives = 174/363 (47.93%), Query Frame = 0


Query: 64  VVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRVK---PKTTSFDPSLSSSFSLLPCNNPLC 123
           +++L IGTP QP   ++DTGS L W QC    +     T  F+P  SSSFS LPC++ LC
Sbjct: 96  LMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLC 155

Query: 124 KPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGCTK--- 183
           +       L +    N  C Y+Y Y DG+  +G++  E     + ++ P +  GC +   
Sbjct: 156 Q------ALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGS-VSIPNITFGCGENNQ 215

Query: 184 --ASSENRGILGMNAGRLSFISQARINKFSYCIP--GSTRPGSTGLFYLGQNPNSAHFRY 243
                   G++GM  G LS  SQ  + KFSYC+   GS+ P +  L  L  +  +     
Sbjct: 216 GFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS--- 275

Query: 244 VPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFK-PDRTGSGQTMVDSGSDL 303
            P  TL +S + P      Y + + G+ +G +RL I  + F      G+G  ++DSG+ L
Sbjct: 276 -PNTTLIQSSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 335

Query: 304 TYLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENG 363
           TY V+ AY  VR+  +  +   +  G   ++  D+CF   T +  +  +I      F+ G
Sbjct: 336 TYFVNNAYQSVRQEFISQINLPVVNG--SSSGFDLCFQ--TPSDPSNLQIPTFVMHFDGG 395

Query: 364 VEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGG 416
              L    E        G+ C+A+G S +   G +I GN+ Q+NM V YD  N  + F  
Sbjct: 396 DLEL--PSENYFISPSNGLICLAMGSSSQ---GMSIFGNIQQQNMLVVYDTGNSVVSFAS 434

[Close]

BLAST of Sed0009661.1 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.6e-39
Identity = 115/366 (31.42%), Postives = 178/366 (48.63%), Query Frame = 0


Query: 64  VVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRVK---PKTTSFDPSLSSSFSLLPCNNPLC 123
           ++++ IGTP      ++DTGS L W QC    +     T  F+P  SSSFS LPC +  C
Sbjct: 97  LMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYC 156

Query: 124 KPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGCTK--- 183
           +       LP+    N  C Y+Y Y DG+  +G +  E F      + P +  GC +   
Sbjct: 157 Q------DLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFET-SSVPNIAFGCGEDNQ 216

Query: 184 --ASSENRGILGMNAGRLSFISQARINKFSYCIP--GSTRPGSTGLFYLGQNPNSAHFRY 243
                   G++GM  G LS  SQ  + +FSYC+   GS+ P +  L              
Sbjct: 217 GFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGS--- 276

Query: 244 VPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLT 303
            P  TL  S    +L+P  Y + ++GI +GG  L I S+ F+    G+G  ++DSG+ LT
Sbjct: 277 -PSTTLIHS----SLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLT 336

Query: 304 YLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF----DGWTAAAAAGRRIGDMSFEF 363
           YL  +AY+ V +A    +   +    E ++    CF    DG T       ++ ++S +F
Sbjct: 337 YLPQDAYNAVAQAFTDQIN--LPTVDESSSGLSTCFQQPSDGSTV------QVPEISMQF 396

Query: 364 ENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIG 416
           + GV + +G+ + +L    +GV C+A+G S +LGI  +I GN+ Q+   V YDL N  + 
Sbjct: 397 DGGV-LNLGE-QNILISPAEGVICLAMGSSSQLGI--SIFGNIQQQETQVLYDLQNLAVS 435

[Close]

BLAST of Sed0009661.1 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.4e-32
Identity = 101/358 (28.21%), Postives = 167/358 (46.65%), Query Frame = 0


Query: 69  IGTPPQPTDFVLDTGSQLSWIQCH---DRVKPKTTSFDPSLSSSFSLLPCNNPLCKPRIP 128
           +GTP +    VLDTGS ++WIQC    D  +     F+P+ SS++  L C+ P C     
Sbjct: 168 VGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCS---- 227

Query: 129 DFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGCTKAS----SE 188
              L TS  ++  C Y   Y DG+   G L  +     N      V LGC   +    + 
Sbjct: 228 --LLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNEGLFTG 287

Query: 189 NRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFY----LGQNPNSAHFRYVPML 248
             G+LG+  G LS  +Q +   FSYC+       S+ L +    LG    +A     P+L
Sbjct: 288 AAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFNSVQLGGGDATA-----PLL 347

Query: 249 TLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYLVD 308
                 R+  +D   Y + + G  +GG ++ +  A+F  D +GSG  ++D G+ +T L  
Sbjct: 348 ------RNKKIDTF-YYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQT 407

Query: 309 EAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENGVEVLV 368
           +AY+ +R+A ++L    +KKG    ++ D C+D    ++ +  ++  ++F F  G  + +
Sbjct: 408 QAYNSLRDAFLKLT-VNLKKGSSSISLFDTCYD---FSSLSTVKVPTVAFHFTGGKSLDL 467

Query: 369 GKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAVC 416
                ++   + G  C A   +       +IIGNV Q+   + YDL+   IG  G  C
Sbjct: 468 PAKNYLIPVDDSGTFCFAFAPTSS---SLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Sed0009661.1 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.6e-31
Identity = 108/361 (29.92%), Postives = 177/361 (49.03%), Query Frame = 0


Query: 67  LPIGTPPQPTDFVLDTGSQLSWIQCHDRVKPKTTS---FDPSLSSSFSLLPCNNPLCKPR 126
           L +GTP +    VLDTGS + W+QC    +  + S   FDP  S +++ +PC++P C+ R
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCR-R 205

Query: 127 IPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGCTKASS--- 186
           +      T   + + C Y   Y DG+   G+   E            V LGC   +    
Sbjct: 206 LDSAGCNT---RRKTCLYQVSYGDGSFTVGDFSTETLTFRRN-RVKGVALGCGHDNEGLF 265

Query: 187 -ENRGILGMNAGRLSFISQA--RIN-KFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVPM 246
               G+LG+  G+LSF  Q   R N KFSYC+   +          G    S   R+ P+
Sbjct: 266 VGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPL 325

Query: 247 LTLPKSQRSPNLDPLAYTLPMKGIKIGGSRL-NISSAVFKPDRTGSGQTMVDSGSDLTYL 306
           L+ PK      LD   Y + + GI +GG+R+  +++++FK D+ G+G  ++DSG+ +T L
Sbjct: 326 LSNPK------LDTF-YYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRL 385

Query: 307 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENGVEV 366
           +  AY  +R+A  R+    +K+  ++ ++ D CFD    +     ++  +   F  G +V
Sbjct: 386 IRPAYIAMRDAF-RVGAKTLKRAPDF-SLFDTCFD---LSNMNEVKVPTVVLHF-RGADV 445

Query: 367 LVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAVC 417
            +     ++     G  C A   +G +G G +IIGN+ Q+   V YDLA+ R+GF    C
Sbjct: 446 SLPATNYLIPVDTNGKFCFAF--AGTMG-GLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 485

[Close]

BLAST of Sed0009661.1 vs. ExPASy TrEMBL
Match: A0A6J1F8Z2 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.2e-190
Identity = 341/425 (80.24%), Postives = 371/425 (87.29%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYS 60
           M+  LF LSL SL FSHS+SLSLSFPLSLSK  SP S     L+S  +++ S KL FKYS
Sbjct: 1   MLLSLFYLSLLSLSFSHSHSLSLSFPLSLSKRPSPVSSSVSPLFSSLSAYGSVKLPFKYS 60

Query: 61  TALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRV-----KPKTTSFDPSLSSSFSLLPC 120
           TALVVSLPIGTPPQPTD VLDTGSQLSWIQCH +V     KPKTTSFDPSLSSSFSLLPC
Sbjct: 61  TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKVHKKLLKPKTTSFDPSLSSSFSLLPC 120

Query: 121 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 180
           N+PLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN  TTPPVILGC
Sbjct: 121 NHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTPPVILGC 180

Query: 181 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVP 240
            +AS+ENRGILGMN GRLSF+SQA+I+KFSYC+PG T P STGLFYLG NPNSA+F+Y+ 
Sbjct: 181 AQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSANFKYIS 240

Query: 241 MLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYL 300
           +LT PKSQRSPNLDPLAYTLP+KGIKI G+RLNISSAVFKPDR+GSGQTM+DSGSDLTYL
Sbjct: 241 LLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSGSDLTYL 300

Query: 301 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF-DGWTAAAAAGRRIGDMSFEFENGVE 360
           VDEAY+KV+E +V+LVGP+MKKGYEYAAVADMCF DG T  A  GRRI DMSFEFENGVE
Sbjct: 301 VDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGET--AEVGRRIRDMSFEFENGVE 360

Query: 361 VLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAV 420
           + VGKGEGVLTEVEKGVKCV  GRSGRLGI SNIIG VHQ+N W+EYDLANRRIGFGGA 
Sbjct: 361 ISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWIEYDLANRRIGFGGAD 420

[Close]

BLAST of Sed0009661.1 vs. ExPASy TrEMBL
Match: A0A6J1J2U3 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.9e-189
Identity = 340/425 (80.00%), Postives = 370/425 (87.06%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHDSAKLLFKYS 60
           M+  LF +SL SL FSHS+SL LSFPLSLSK  SP SP    L+S  +S+ S KL FKYS
Sbjct: 1   MLLSLFYISLLSLSFSHSHSLPLSFPLSLSKRPSPVSP----LFSSLSSYGSVKLPFKYS 60

Query: 61  TALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDR-----VKPKTTSFDPSLSSSFSLLPC 120
           TALVVSLPIGTPPQPTD VLDTGSQLSWIQCH +     +KPKT SFDPSLSSSFSLLPC
Sbjct: 61  TALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHRKLHKKLLKPKTASFDPSLSSSFSLLPC 120

Query: 121 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 180
           N+PLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF  SN  TTPPVILGC
Sbjct: 121 NHPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFTFSNSRTTPPVILGC 180

Query: 181 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYVP 240
            +AS+ENRGILGMN GRLSF+SQA+I+KFSYC+PG T P STGLFYLG NPNSA+F+Y+ 
Sbjct: 181 AQASTENRGILGMNTGRLSFVSQAKISKFSYCVPGRTGPDSTGLFYLGDNPNSANFKYIS 240

Query: 241 MLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYL 300
           +LT PKSQRSPNLDPLAYTLP+KGIKI G+RLNISSAVFKPDR+GSGQTM+DSGSDLTYL
Sbjct: 241 LLTFPKSQRSPNLDPLAYTLPLKGIKIAGNRLNISSAVFKPDRSGSGQTMIDSGSDLTYL 300

Query: 301 VDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCF-DGWTAAAAAGRRIGDMSFEFENGVE 360
           VDEAY+KV+E +V+LVGP+MKKGYEYAAVADMCF DG T  A  GRRI DMSFEFENGVE
Sbjct: 301 VDEAYEKVKEEIVKLVGPLMKKGYEYAAVADMCFNDGET--AEVGRRIRDMSFEFENGVE 360

Query: 361 VLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAV 420
           + VGKGEGVLTEVEKGVKCV  GRSGRLGI SNIIG VHQ+N WVEYDLANRRIGFGGA 
Sbjct: 361 ISVGKGEGVLTEVEKGVKCVGFGRSGRLGIASNIIGTVHQKNTWVEYDLANRRIGFGGAD 419

[Close]

BLAST of Sed0009661.1 vs. ExPASy TrEMBL
Match: A0A5A7U1M2 (Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00330 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.9e-182
Identity = 334/430 (77.67%), Postives = 363/430 (84.42%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLY-PFLYS-KTTSHDSAKLLFK 60
           M+  LFSLSLF+L FS SNS+SL FPLSLS+  S  SP+Y   LY+ K +SH S KL FK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  Y-STALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRV--------KPKTTSFDPSLSSS 120
           Y STALVVSLPIGTPPQPTD VLDTGSQLSWIQCHD+V        KPKT SFDPSLSSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120

Query: 121 FSLLPCNNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTP 180
           FSLLPCN+P+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+LSN L+TP
Sbjct: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180

Query: 181 PVILGCTKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSA 240
           PVILGC +AS+ENRGILGMN GRLSFISQA+I+KFSYC+P  T    TGLFYLG NPNS+
Sbjct: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240

Query: 241 HFRYVPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSG 300
            F+YV MLT P+SQ SPNLDPLAYTLPMKGIKI G RLNIS A FKPD  GSGQTM+DSG
Sbjct: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEF 360
           SDLTYLVDEAY+KV+E +VRLVG  MKKGY YAAVADMCFD     A  GRRIG +SFEF
Sbjct: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDA-RVTAEVGRRIGGISFEF 360

Query: 361 ENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIG 420
           +NGVE+LVG+GEGVLTEVEKGVKCV  GRS RLGIGSNIIG VHQ+NMWVEYDL NRRIG
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

[Close]

BLAST of Sed0009661.1 vs. ExPASy TrEMBL
Match: A0A1S3C4D2 (aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.9e-182
Identity = 334/430 (77.67%), Postives = 363/430 (84.42%), Query Frame = 0


Query: 1   MISFLFSLSLFSLFFSHSNSLSLSFPLSLSKTTSPKSPLY-PFLYS-KTTSHDSAKLLFK 60
           M+  LFSLSLF+L FS SNS+SL FPLSLS+  S  SP+Y   LY+ K +SH S KL FK
Sbjct: 1   MLLILFSLSLFTLPFSQSNSVSLPFPLSLSEKPSNISPIYGSQLYAKKPSSHGSFKLPFK 60

Query: 61  Y-STALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDRV--------KPKTTSFDPSLSSS 120
           Y STALVVSLPIGTPPQPTD VLDTGSQLSWIQCHD+V        KPKT SFDPSLSSS
Sbjct: 61  YSSTALVVSLPIGTPPQPTDLVLDTGSQLSWIQCHDKVKKKLPPLPKPKTASFDPSLSSS 120

Query: 121 FSLLPCNNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTP 180
           FSLLPCN+P+CKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKF+LSN L+TP
Sbjct: 121 FSLLPCNHPICKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFSLSNSLSTP 180

Query: 181 PVILGCTKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSA 240
           PVILGC +AS+ENRGILGMN GRLSFISQA+I+KFSYC+P  T    TGLFYLG NPNS+
Sbjct: 181 PVILGCAQASTENRGILGMNKGRLSFISQAKISKFSYCVPARTGSNPTGLFYLGDNPNSS 240

Query: 241 HFRYVPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSG 300
            F+YV MLT P+SQ SPNLDPLAYTLPMKGIKI G RLNIS A FKPD  GSGQTM+DSG
Sbjct: 241 RFKYVTMLTFPESQSSPNLDPLAYTLPMKGIKIAGKRLNISPAAFKPDAGGSGQTMIDSG 300

Query: 301 SDLTYLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEF 360
           SDLTYLVDEAY+KV+E +VRLVG  MKKGY YAAVADMCFD     A  GRRIG +SFEF
Sbjct: 301 SDLTYLVDEAYEKVKEEVVRLVGAKMKKGYVYAAVADMCFDA-RVTAEVGRRIGGISFEF 360

Query: 361 ENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIG 420
           +NGVE+LVG+GEGVLTEVEKGVKCV  GRS RLGIGSNIIG VHQ+NMWVEYDL NRRIG
Sbjct: 361 DNGVEILVGRGEGVLTEVEKGVKCVGFGRSERLGIGSNIIGTVHQQNMWVEYDLTNRRIG 420

[Close]

BLAST of Sed0009661.1 vs. ExPASy TrEMBL
Match: A0A6J1K7E8 (aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111492941 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.4e-168
Identity = 313/425 (73.65%), Postives = 348/425 (81.88%), Query Frame = 0


Query: 2   ISFLFSLSLFSLFFSHSNSLSLSFPL-SLSKTTSPK-SPLYPFLYSKTTSHDSAKLLFKY 61
           +S LF ++LFSL FS SNSLSLSFPL S S +  P  SPL+  LYSK  S   AKL FKY
Sbjct: 3   LSLLF-VALFSLLFSASNSLSLSFPLTSRSLSEEPSISPLHLSLYSKAASKPPAKLPFKY 62

Query: 62  STALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDR-----VKPKTTSFDPSLSSSFSLLP 121
           S ALVVSLP+GTPPQ  D VLDTGSQLSWIQCH +     V+P+   F+PSLSS+FS LP
Sbjct: 63  SNALVVSLPMGTPPQLMDMVLDTGSQLSWIQCHGKVNGKSVQPRIKWFNPSLSSTFSFLP 122

Query: 122 CNNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILG 181
           CN+ LCKPRIPDFTLPTSCD  R CHYSYFYADGTLAEGNLV EKF+     T  PV +G
Sbjct: 123 CNSSLCKPRIPDFTLPTSCDPRRHCHYSYFYADGTLAEGNLVTEKFS----FTASPVAIG 182

Query: 182 CTKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNSAHFRYV 241
           C K S+ENRGILGMN G LSFISQA+I+KFSYC+PG +R   TGLFYLG NPNS  F+YV
Sbjct: 183 CVKPSAENRGILGMNTGHLSFISQAKISKFSYCVPGRSRSDLTGLFYLGDNPNSGKFKYV 242

Query: 242 PMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTY 301
            MLT P+SQ SPNLD LAYTLPMKGI+IG   L IS AVFKPD TGSGQTM+DSGS+LTY
Sbjct: 243 NMLTFPESQNSPNLDKLAYTLPMKGIRIGRVHLKISPAVFKPDPTGSGQTMIDSGSELTY 302

Query: 302 LVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENGVE 361
           LVDEAY+KVR  +VRLVGPMMKKGYEYA+V+DMCFD    AAAAGRRIGDM F+FENGVE
Sbjct: 303 LVDEAYNKVRAEIVRLVGPMMKKGYEYASVSDMCFDA-AMAAAAGRRIGDMWFQFENGVE 362

Query: 362 VLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGGAV 420
           +LVGKGEG+LTEVEKGVKCV IGRSGRLG  SN+IGNVHQ+NMWVEYDLAN+R+GFGGAV
Sbjct: 363 ILVGKGEGLLTEVEKGVKCVGIGRSGRLGTESNMIGNVHQQNMWVEYDLANKRVGFGGAV 421

[Close]

BLAST of Sed0009661.1 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 511.1 bits (1315), Expect = 8.2e-145
Identity = 268/427 (62.76%), Postives = 318/427 (74.47%), Query Frame = 0


Query: 4   FLFSLSLFSLFFSHSNSLSLSFPLS---LSKTTSPKSPLYPFLYSKTTSHDSAKLLF--- 63
           ++F    +S+  S S+SLSL FPL+   L+ TT+  S     L  +  S  S+   F   
Sbjct: 14  YIFFFFCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPPSSPYTFRSN 73

Query: 64  -KYSTALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDR-----VKPKTTSFDPSLSSSFS 123
            KYS AL++SLPIGTP Q  + VLDTGSQLSWIQCH +     + P TTSFDPSLSSSFS
Sbjct: 74  IKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSFDPSLSSSFS 133

Query: 124 LLPCNNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPV 183
            LPC++PLCKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EKF  SN  TTPP+
Sbjct: 134 DLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTFSNSQTTPPL 193

Query: 184 ILGCTKASSENRGILGMNAGRLSFISQARINKFSYCIP-GSTRPG--STGLFYLGQNPNS 243
           ILGC K S++ +GILGMN GRLSFISQA+I+KFSYCIP  S RPG  STG FYLG NPNS
Sbjct: 194 ILGCAKESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNRPGLASTGSFYLGDNPNS 253

Query: 244 AHFRYVPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDS 303
             F+YV +LT P+SQR PNLDPLAYT+P++GI+IG  RLNI  +VF+PD  GSGQTMVDS
Sbjct: 254 RGFKYVSLLTFPQSQRMPNLDPLAYTVPLQGIRIGQKRLNIPGSVFRPDAGGSGQTMVDS 313

Query: 304 GSDLTYLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFE 363
           GS+ T+LVD AYDKV+E +VRLVG  +KKGY Y + ADMCFDG   +   GR IGD+ FE
Sbjct: 314 GSEFTHLVDVAYDKVKEEIVRLVGSRLKKGYVYGSTADMCFDG-NHSMEIGRLIGDLVFE 373

Query: 364 FENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRI 416
           F  GVE+LV K + +L  V  G+ CV IGRS  LG  SNIIGNVHQ+N+WVE+D+ NRR+
Sbjct: 374 FGRGVEILVEK-QSLLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRV 433

[Close]

BLAST of Sed0009661.1 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 491.1 bits (1263), Expect = 8.8e-139
Identity = 267/424 (62.97%), Postives = 304/424 (71.70%), Query Frame = 0


Query: 10  LFSLFF----SHSNSLSLSFPLS---LSKTTSPKSPLYPFLYSKTTSHDSA----KLLFK 69
           LF  FF    S S SLSL  PL+   +S TT+        L  K  S  S     +  FK
Sbjct: 8   LFFFFFLNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSPPYNFRSRFK 67

Query: 70  YSTALVVSLPIGTPPQPTDFVLDTGSQLSWIQCHDR---VKPKTTSFDPSLSSSFSLLPC 129
           YS AL++SLPIGTPPQ    VLDTGSQLSWIQCH +    KPK TSFDPSLSSSFS LPC
Sbjct: 68  YSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPK-TSFDPSLSSSFSTLPC 127

Query: 130 NNPLCKPRIPDFTLPTSCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC 189
           ++PLCKPRIPDFTLPTSCD NRLCHYSYFYADGT AEGNLV+EK   SN   TPP+ILGC
Sbjct: 128 SHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEITPPLILGC 187

Query: 190 TKASSENRGILGMNAGRLSFISQARINKFSYCI-PGSTRPG--STGLFYLGQNPNSAHFR 249
              SS++RGILGMN GRLSF+SQA+I+KFSYCI P S RPG   TG FYLG NPNS  F+
Sbjct: 188 ATESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTPTGSFYLGDNPNSHGFK 247

Query: 250 YVPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDL 309
           YV +LT P+SQR PNLDPLAYT+PM GI+ G  +LNIS +VF+PD  GSGQTMVDSGS+ 
Sbjct: 248 YVSLLTFPESQRMPNLDPLAYTVPMIGIRFGLKKLNISGSVFRPDAGGSGQTMVDSGSEF 307

Query: 310 TYLVDEAYDKVREAMVRLVGPMMKKGYEYAAVADMCFDGWTAAAAAGRRIGDMSFEFENG 369
           T+LVD AYDKVR  ++  VG  +KKGY Y   ADMCFDG    A   R IGD+ F F  G
Sbjct: 308 THLVDAAYDKVRAEIMTRVGRRLKKGYVYGGTADMCFDG--NVAMIPRLIGDLVFVFTRG 367

Query: 370 VEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIGFGG 417
           VE+LV K E VL  V  G+ CV IGRS  LG  SNIIGNVHQ+N+WVE+D+ NRR+GF  
Sbjct: 368 VEILVPK-ERVLVNVGGGIHCVGIGRSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFAK 427

[Close]

BLAST of Sed0009661.1 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.4e-64
Identity = 152/426 (35.68%), Postives = 222/426 (52.11%), Query Frame = 0


Query: 16  SHSNSLSLSFPLSLSKTTSPKSPLYPFLYSKTTSHD---SAKLLFKYSTALVVSLPIGTP 75
           S S+S S SF  S   ++S    L   L ++ T  D   + KL F ++  L V+L +GTP
Sbjct: 23  SSSSSSSSSFSFSSFSSSSSSQTLVLPLKTRITPTDHRPTDKLHFHHNVTLTVTLTVGTP 82

Query: 76  PQPTDFVLDTGSQLSWIQCHDRVKPK-TTSFDPSLSSSFSLLPCNNPLCKPRIPDFTLPT 135
           PQ    V+DTGS+LSW++C+    P    +FDP+ SSS+S +PC++P C+ R  DF +P 
Sbjct: 83  PQNISMVIDTGSELSWLRCNRSSNPNPVNNFDPTRSSSYSPIPCSSPTCRTRTRDFLIPA 142

Query: 136 SCDQNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC--------TKASSENR 195
           SCD ++LCH +  YAD + +EGNL  E F   N      +I GC         +  ++  
Sbjct: 143 SCDSDKLCHATLSYADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTT 202

Query: 196 GILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNS--AHFRYVPMLTLPK 255
           G+LGMN G LSFISQ    KFSYCI G+      G   LG +  +      Y P++ +  
Sbjct: 203 GLLGMNRGSLSFISQMGFPKFSYCISGT--DDFPGFLLLGDSNFTWLTPLNYTPLIRI-- 262

Query: 256 SQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLTYLVDEAYD 315
           S   P  D +AYT+ + GIK+ G  L I  +V  PD TG+GQTMVDSG+  T+L+   Y 
Sbjct: 263 STPLPYFDRVAYTVQLTGIKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYT 322

Query: 316 KVREAMVRLVGPMM----KKGYEYAAVADMCFDGWTAAAAAG--RRIGDMSFEFENGVEV 375
            +R   +     ++       + +    D+C+        +G   R+  +S  FE G E+
Sbjct: 323 ALRSHFLNRTNGILTVYEDPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFE-GAEI 382

Query: 376 LVGKGEGVLTEV------EKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLANRRIG 416
            V  G+ +L  V         V C   G S  +G+ + +IG+ HQ+NMW+E+DL   RIG
Sbjct: 383 AV-SGQPLLYRVPHLTVGNDSVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIG 442

[Close]

BLAST of Sed0009661.1 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.4e-64
Identity = 153/430 (35.58%), Postives = 237/430 (55.12%), Query Frame = 0


Query: 7   SLSLFSLFFSHSNSLSLSFPLSLSKTTSP-KSPLYPFLYSKTTSHDSAKLLFKYSTALVV 66
           SLSL S  F   + L L FPL+  KT+S  ++ L+     K     S KL F+++  L V
Sbjct: 9   SLSL-SKNFLRISVLLLIFPLTFCKTSSTNQTLLFSLKTQKLPQSSSDKLSFRHNVTLTV 68

Query: 67  SLPIGTPPQPTDFVLDTGSQLSWIQCHDRVKPKTTS-FDPSLSSSFSLLPCNNPLCKPRI 126
           +L +G PPQ    VLDTGS+LSW+ C  +  P   S F+P  SS++S +PC++P+C+ R 
Sbjct: 69  TLAVGDPPQNISMVLDTGSELSWLHC--KKSPNLGSVFNPVSSSTYSPVPCSSPICRTRT 128

Query: 127 PDFTLPTSCD-QNRLCHYSYFYADGTLAEGNLVREKFALSNYLTTPPVILGC-------- 186
            D  +P SCD +  LCH +  YAD T  EGNL  E F + + +T P  + GC        
Sbjct: 129 RDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIGS-VTRPGTLFGCMDSGLSSN 188

Query: 187 TKASSENRGILGMNAGRLSFISQARINKFSYCIPGSTRPGSTGLFYLGQNPNS--AHFRY 246
           ++  +++ G++GMN G LSF++Q   +KFSYCI GS    S+G   LG    S     +Y
Sbjct: 189 SEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCISGS---DSSGFLLLGDASYSWLGPIQY 248

Query: 247 VPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPDRTGSGQTMVDSGSDLT 306
            P++   +S   P  D +AYT+ ++GI++G   L++  +VF PD TG+GQTMVDSG+  T
Sbjct: 249 TPLVL--QSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGTQFT 308

Query: 307 YLVDEAYDKVREAMVRLVGPMMK----KGYEYAAVADMCF--DGWTAAAAAGRRIGDMSF 366
           +L+   Y  ++   +     +++      + +    D+C+     T    +G  +  + F
Sbjct: 309 FLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVSLMF 368

Query: 367 ---EFENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVHQRNMWVEYDLA 415
              E     + L+ +  G  +E ++ V C   G S  LGI + +IG+ HQ+N+W+E+DLA
Sbjct: 369 RGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEFDLA 428

[Close]

BLAST of Sed0009661.1 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 148.7 bits (374), Expect = 1.1e-35
Identity = 116/383 (30.29%), Postives = 177/383 (46.21%), Query Frame = 0


Query: 65  VSLPIGTPPQPTDFVLDTGSQLSWIQCH-----DRVKPKTTSFDPSLSSSFSLLPCNNPL 124
           V L IG PPQ    + DTGS L W++C          P T  F P  SS+FS   C +P+
Sbjct: 86  VDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSPATVFF-PRHSSTFSPAHCYDPV 145

Query: 125 CKPRIPDFTLPTSCDQNRL---CHYSYFYADGTLAEGNLVRE----KFALSNYLTTPPVI 184
           C+  +P       C+  R+   CHY Y YADG+L  G   RE    K +         V 
Sbjct: 146 CR-LVPKPDRAPICNHTRIHSTCHYEYGYADGSLTSGLFARETTSLKTSSGKEARLKSVA 205

Query: 185 LGC--------TKASSEN--RGILGMNAGRLSFISQARI---NKFSYCIPGST-RPGSTG 244
            GC           +S N   G++G+  G +SF SQ      NKFSYC+   T  P  T 
Sbjct: 206 FGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLGRRFGNKFSYCLMDYTLSPPPTS 265

Query: 245 LFYLGQNPNS-AHFRYVPMLTLPKSQRSPNLDPLAYTLPMKGIKIGGSRLNISSAVFKPD 304
              +G   +  +   + P+LT P       L P  Y + +K + + G++L I  ++++ D
Sbjct: 266 YLIIGNGGDGISKLFFTPLLTNP-------LSPTFYYVKLKSVFVNGAKLRIDPSIWEID 325

Query: 305 RTGSGQTMVDSGSDLTYLVDEAYDKVREAMVRLV----GPMMKKGYEYAAVADMCFDGWT 364
            +G+G T+VDSG+ L +L + AY  V  A+ R V       +  G+      D+C +  +
Sbjct: 326 DSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIADALTPGF------DLCVN-VS 385

Query: 365 AAAAAGRRIGDMSFEFENGVEVLVGKGEGVLTEVEKGVKCVAIGRSGRLGIGSNIIGNVH 417
                 + +  + FEF  G  V V        E E+ ++C+AI +S    +G ++IGN+ 
Sbjct: 386 GVTKPEKILPRLKFEFSGGA-VFVPPPRNYFIETEEQIQCLAI-QSVDPKVGFSVIGNLM 445

The following BLAST results are available for this feature:

BLAST of Sed0009661.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878004.1	3.1e-194	81.84	aspartic proteinase PCS1 [Benincasa hispida]	[more]
XP_022934878.1	4.6e-190	80.24	aspartic proteinase PCS1-like [Cucurbita moschata]	[more]
KAG6581233.1	1.3e-189	80.24	Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022983616.1	3.9e-189	80.00	aspartic proteinase PCS1-like [Cucurbita maxima]	[more]
XP_023526382.1	1.1e-188	79.58	aspartic proteinase PCS1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sed0009661.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	2.0e-63	35.68	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	4.1e-40	31.13	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.6e-39	31.42	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LS40	2.4e-32	28.21	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.6e-31	29.92	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]

BLAST of Sed0009661.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F8Z2	2.2e-190	80.24	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111441919 PE=3...	[more]
A0A6J1J2U3	1.9e-189	80.00	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111482170 PE=3 S...	[more]
A0A5A7U1M2	2.9e-182	77.67	Aspartic proteinase PCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3C4D2	2.9e-182	77.67	aspartic proteinase PCS1 OS=Cucumis melo OX=3656 GN=LOC103496869 PE=3 SV=1	[more]
A0A6J1K7E8	1.4e-168	73.65	aspartic proteinase PCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111492941 PE=3 S...	[more]

BLAST of Sed0009661.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37540.1	8.2e-145	62.76	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	8.8e-139	62.97	Eukaryotic aspartyl protease family protein	[more]
AT5G02190.1	1.4e-64	35.68	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	2.4e-64	35.58	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	1.1e-35	30.29	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 69..89 score: 46.65 coord: 284..295 score: 31.63 coord: 387..402 score: 29.1
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 22..416
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 252..410 e-value: 5.1E-33 score: 114.1
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 64..223 e-value: 2.8E-35 score: 122.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 45..221 e-value: 9.7E-34 score: 119.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 222..419 e-value: 9.3E-41 score: 141.4
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 64..415
None	No IPR available	PANTHER	PTHR47965:SF51	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 22..416
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 78..89
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 63..411 score: 32.38158
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 64..415 e-value: 1.63477E-71 score: 224.064

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0009661	Sed0009661	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0009661.1-five_prime_utr	Sed0009661.1-five_prime_utr-LG05:38712791..38712856	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0009661.1-exon	Sed0009661.1-exon-LG05:38712791..38714286	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0009661.1-cds	Sed0009661.1-cds-LG05:38712857..38714116	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0009661.1-three_prime_utr	Sed0009661.1-three_prime_utr-LG05:38714117..38714286	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0009661.1	Sed0009661.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity