Sed0006822.1 (mRNA) Chayote v1

BLAST of Sed0006822.1 vs. NCBI nr
Match: KAG7036897.1 (Protein ALP1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 707.6 bits (1825), Expect = 6.0e-200
Identity = 346/391 (88.49%), Postives = 359/391 (91.82%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  GL S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVDAEMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+GDE +DGN+P  MS
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVP--MS 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHDADYKQQICDVYDSKGAYLRDKLSLLFI
Sbjct: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of Sed0006822.1 vs. NCBI nr
Match: XP_022948999.1 (protein ALP1-like [Cucurbita moschata])

HSP 1 Score: 705.3 bits (1819), Expect = 3.0e-199
Identity = 344/391 (87.98%), Postives = 359/391 (91.82%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  GL S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTA+SSNFTFLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAESSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVDAEMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+GDE +DGN+P+SM 
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVPMSM- 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
            EHDADYKQQICDVYDSKGAYLRDKLSLLFI
Sbjct: 361 -EHDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of Sed0006822.1 vs. NCBI nr
Match: KAG6607212.1 (Protein ALP1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 704.5 bits (1817), Expect = 5.1e-199
Identity = 345/391 (88.24%), Postives = 358/391 (91.56%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  GL S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVDAEMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+ DE +DGN+P  MS
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVRDEMEDGNVP--MS 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHDADYKQQICDVYDSKGAYLRDKLSLLFI
Sbjct: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of Sed0006822.1 vs. NCBI nr
Match: XP_022997817.1 (protein ALP1-like [Cucurbita maxima])

HSP 1 Score: 704.1 bits (1816), Expect = 6.6e-199
Identity = 344/391 (87.98%), Postives = 358/391 (91.56%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  GL S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKSSNF FLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSSNFMFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVDAEMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIITGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+GDE +DGN+P  MS
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVP--MS 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHDADYKQQICDVYDSKGAYLR+KLSLLFI
Sbjct: 361 IEHDADYKQQICDVYDSKGAYLREKLSLLFI 389

BLAST of Sed0006822.1 vs. NCBI nr
Match: XP_023523098.1 (protein ALP1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023523103.1 protein ALP1-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023523108.1 protein ALP1-like isoform X3 [Cucurbita pepo subsp. pepo] >XP_023523112.1 protein ALP1-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.0 bits (1813), Expect = 1.5e-198
Identity = 343/391 (87.72%), Postives = 357/391 (91.30%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  G  S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGFHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPS+E EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSSEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVD EMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDTEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+GDE +DGN+P  MS
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVP--MS 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHDADYKQQICDVYDSKGAYLRDKLSLLFI
Sbjct: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of Sed0006822.1 vs. ExPASy Swiss-Prot
Match: Q9M2U3 (Protein ALP1-like OS=Arabidopsis thaliana OX=3702 GN=At3g55350 PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.7e-128
Identity = 230/405 (56.79%), Postives = 296/405 (73.09%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLD-------------ANGGTASDSSEKDD-----ALDWWDDFSR 60
           MGPI+ ++KKK+ E K+D             A+   A ++++ DD     +LDWWD FSR
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RINGLSSPLKGSDRFKSIFKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAV 120
           RI G S+  K    F+S+FK+SRKTFDYIC LVK D TAK +NF+  NG PLSL D+VAV
Sbjct: 61  RIYGGSTDPK---TFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKF 180
           ALRRLGSGESL +IG++FG+N STVSQ+TWRFVESMEER + HL WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGF 240
           EKI GLPNCCG+ID THI M LPA +PS+ VWLD EKN SM LQ +VD +MRF D++ G+
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKMSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDG 300
           PG ++D +V ++S F KL EKG+RLNG+ L L + +E+REY+VGDSG+PLLP+L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNI 360
           K     + EFNKRH+E     Q AL+ LK+RWRII+GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 AIDIGDEKDDGNIPLSMSIEHDADYKQQICDVYDSKGAYLRDKLS 388
            ID+ D+  D   PLS   +HD +Y+Q+ C + D   + LRD+LS
Sbjct: 361 IIDMEDQTLDDQ-PLSQ--QHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of Sed0006822.1 vs. ExPASy Swiss-Prot
Match: Q94K49 (Protein ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=ALP1 PE=1 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.5e-92
Identity = 185/398 (46.48%), Postives = 248/398 (62.31%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEK--------DDAL--DWWDDFSRRINGLSSP 60
           M P++  +KKK  +  LD     A +  +K         +A+  DWWD F  R +  S P
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  LKGSDRFKSIFKVSRKTFDYICLLVKDDMTAK-SSNFTFLNGRPLSLYDQVAVALRRLGS 120
                 FK  F+ S+ TF YIC LV++D+ ++  S    + GR LS+  QVA+ALRRL S
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLAS 120

Query: 121 GESLVMIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLP 180
           G+S V +G +FGV  STVSQVTWRF+E++EER   HL WP ++  + ++KSKFE++ GLP
Sbjct: 121 GDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGLP 180

Query: 181 NCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDS 240
           NCCG+IDTTHI M LPA   S + W DQEKN+SM LQ + D EMRF +++TG+PG M+ S
Sbjct: 181 NCCGAIDTTHIIMTLPAVQASDD-WCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVS 240

Query: 241 LVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSK 300
            + + S F KLCE  + L+G    L   ++IREYVVG   YPLLP+L+TP+D      S 
Sbjct: 241 KLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSM 300

Query: 301 AEFNKRHAETRVVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDE 360
             FN+RH + R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNI ID GD 
Sbjct: 301 VAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 360

Query: 361 KDDGNIPLSMSIEHDADYKQQICDVYDSKGAYLRDKLS 388
             + ++PLS    HD+ Y  + C   +  G+ LR  L+
Sbjct: 361 LQE-DVPLSG--HHDSGYADRYCKQTEPLGSELRGCLT 391

BLAST of Sed0006822.1 vs. ExPASy Swiss-Prot
Match: Q6AZB8 (Putative nuclease HARBI1 OS=Danio rerio OX=7955 GN=harbi1 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.4e-28
Identity = 82/297 (27.61%), Postives = 143/297 (48.15%), Query Frame = 0

Query: 55  DRFKSIFKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLV 114
           D   + F   R+   Y+  L+KD +  ++        R +S   Q+  AL    SG    
Sbjct: 33  DFLLNTFGFPREFIYYLVELLKDSLLRRTQ-----RSRAISPDVQILAALGFYTSGSFQS 92

Query: 115 MIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGS 174
            +GD+ G++ +++S+      +++ E+    + +   E    Q K +F +I G+PN  G 
Sbjct: 93  KMGDAIGISQASMSRCVSNVTKALIEKAPEFIGFTRDEATKQQFKDEFYRIAGIPNVTGV 152

Query: 175 IDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQS 234
           +D  HI +  P +D SS  +++++  HS+  Q++ DA        T +PG ++D  VF+ 
Sbjct: 153 VDCAHIAIKAPNADDSS--YVNKKGFHSINCQLVCDARGLLLSAETHWPGSLTDRAVFKQ 212

Query: 235 STFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNK 294
           S   KL E+ E  +  WL            +GD+ YPL  +L+TP    E  P+   +N 
Sbjct: 213 SNVAKLFEEQENDDEGWL------------LGDNRYPLKKWLMTPVQSPE-SPADYRYNL 272

Query: 295 RHAETRVVVQRALASLKERWRIIHG----VMWRPDKHRLPRIILVCCLLHNIAIDIG 348
            H  T  +V R   +++ R+R + G    + + P+K     II  CC+LHNI++  G
Sbjct: 273 AHTTTHEIVDRTFRAIQTRFRCLDGAKGYLQYSPEK--CSHIIQACCVLHNISLQSG 307

BLAST of Sed0006822.1 vs. ExPASy Swiss-Prot
Match: Q17QR8 (Putative nuclease HARBI1 OS=Bos taurus OX=9913 GN=HARBI1 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.3e-26
Identity = 76/301 (25.25%), Postives = 147/301 (48.84%), Query Frame = 0

Query: 92  RPLSLYDQVAVALRRLGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPST 151
           R +S   Q+  AL    SG     +GD+ G++ +++S+      E++ ER  + +H+P+ 
Sbjct: 65  RAISPETQILAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIHFPAD 124

Query: 152 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDA 211
           E  +  +K +F  + G+P   G +D  H+ +  P ++  S  +++++  HS+   ++ D 
Sbjct: 125 EASVQALKDEFYGLAGIPGVIGVVDCMHVAIKAPNAEDLS--YVNRKGLHSLNCLMVCDI 184

Query: 212 EMRFRDILTGFPGKMSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYP 271
                 + T +PG + D +V Q S+     E G      WL            +GDS + 
Sbjct: 185 RGALMTVETSWPGSLQDCVVLQQSSLSSQFEAGMH-KESWL------------LGDSSFF 244

Query: 272 LLPYLVTPYDGKELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHG----VMWRPDKH 331
           L  +L+TP    E  P++  +N  H+ T  V+++   +L  R+R + G    + + P+K 
Sbjct: 245 LRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKS 304

Query: 332 RLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMSIEHDADYKQQICDVYDSKGAYLRDKLS 389
               IIL CC+LHNI+++ G   D  + P++  +E   + + +  +  D +   +R +L 
Sbjct: 305 --SHIILACCVLHNISLEHG--MDVWSSPVTGPVEQPPEEEYEHMESLDLEADRIRQELM 345

BLAST of Sed0006822.1 vs. ExPASy Swiss-Prot
Match: Q96MB7 (Putative nuclease HARBI1 OS=Homo sapiens OX=9606 GN=HARBI1 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.9e-25
Identity = 76/301 (25.25%), Postives = 145/301 (48.17%), Query Frame = 0

Query: 92  RPLSLYDQVAVALRRLGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPST 151
           R +S   QV  AL    SG     +GD+ G++ +++S+      E++ ER  + + +P+ 
Sbjct: 65  RAISPETQVLAALGFYTSGSFQTRMGDAIGISQASMSRCVANVTEALVERASQFIRFPAD 124

Query: 152 EVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDA 211
           E  +  +K +F  + G+P   G +D  H+ +  P ++  S  +++++  HS+   ++ D 
Sbjct: 125 EASIQALKDEFYGLAGMPGVMGVVDCIHVAIKAPNAEDLS--YVNRKGLHSLNCLMVCDI 184

Query: 212 EMRFRDILTGFPGKMSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYP 271
                 + T +PG + D  V Q S+     E G      WL            +GDS + 
Sbjct: 185 RGTLMTVETNWPGSLQDCAVLQQSSLSSQFEAGMH-KDSWL------------LGDSSFF 244

Query: 272 LLPYLVTPYDGKELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHG----VMWRPDKH 331
           L  +L+TP    E  P++  +N  H+ T  V+++   +L  R+R + G    + + P+K 
Sbjct: 245 LRTWLMTPLHIPE-TPAEYRYNMAHSATHSVIEKTFRTLCSRFRCLDGSKGALQYSPEKS 304

Query: 332 RLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMSIEHDADYKQQICDVYDSKGAYLRDKLS 389
               IIL CC+LHNI+++ G   D  + P++  +E   + + +  +  D +   +R +L 
Sbjct: 305 --SHIILACCVLHNISLEHG--MDVWSSPMTGPMEQPPEEEYEHMESLDLEADRIRQELM 345

BLAST of Sed0006822.1 vs. ExPASy TrEMBL
Match: A0A6J1GBJ6 (protein ALP1-like OS=Cucurbita moschata OX=3662 GN=LOC111452471 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.4e-199
Identity = 344/391 (87.98%), Postives = 359/391 (91.82%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  GL S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTA+SSNFTFLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAESSNFTFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVDAEMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIVTGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+GDE +DGN+P+SM 
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVPMSM- 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
            EHDADYKQQICDVYDSKGAYLRDKLSLLFI
Sbjct: 361 -EHDADYKQQICDVYDSKGAYLRDKLSLLFI 389

BLAST of Sed0006822.1 vs. ExPASy TrEMBL
Match: A0A6J1K643 (protein ALP1-like OS=Cucurbita maxima OX=3661 GN=LOC111492660 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 3.2e-199
Identity = 344/391 (87.98%), Postives = 358/391 (91.56%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRG RKKKK E KLDAN  TASDSSEKDDALDWWDDFSRR  GL S L+G D FKSI
Sbjct: 1   MGPIRGSRKKKKLERKLDANASTASDSSEKDDALDWWDDFSRRTIGLHSELEGLDGFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKSSNF FLNGRPLSLYDQVAVALRRLGSG+SLV IG SF
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSSNFMFLNGRPLSLYDQVAVALRRLGSGDSLVTIGYSF 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESME RGLRHLHWPSTE EMAQVK KFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEVRGLRHLHWPSTEEEMAQVKLKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLP  DP+SNVWLD EKNHSMVLQVIVDAEMRFRDI+TG PGKMSD LVFQSS F KL
Sbjct: 181 TMCLPVLDPTSNVWLDAEKNHSMVLQVIVDAEMRFRDIITGLPGKMSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           CEKGERLNGK LE ++ SEIREY++GDSGYPLLPYLVTPYDGKELQPSKAEFNKRH ETR
Sbjct: 241 CEKGERLNGKRLEFINRSEIREYIIGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHTETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALASLKERWRII GVMWRPDKHRLPRIILVCCLLHNI ID+GDE +DGN+P  MS
Sbjct: 301 LVVQRALASLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDVGDEMEDGNVP--MS 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHDADYKQQICDVYDSKGAYLR+KLSLLFI
Sbjct: 361 IEHDADYKQQICDVYDSKGAYLREKLSLLFI 389

BLAST of Sed0006822.1 vs. ExPASy TrEMBL
Match: A0A5D3BNB9 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00830 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 8.1e-195
Identity = 338/391 (86.45%), Postives = 357/391 (91.30%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRGLRKKKK E KLD+N GTASDSSEKDDA+DWWDDFS+R NGL S  KG DRFKSI
Sbjct: 1   MGPIRGLRKKKKLERKLDSN-GTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKS +FTFLNGRPLSL DQVAVALRRLGSGESLV IGDS 
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESMEERGLRHLHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEERGLRHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLPASDP+S VWLD EKNHSMVLQVIVDAEMRFRDILTG PGK+SD LVFQSS F KL
Sbjct: 181 TMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           C+KGERLNGK LEL D SEI+EY+VGDSGYPLL YLVTPYDGKEL  SKAEFNKRH  TR
Sbjct: 241 CDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQ+ALA LKERWRII GVMWRPDKHRLPRIILVCCLLHNI IDIGDE +DG++PL  S
Sbjct: 301 LVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPL--S 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHD DYKQQ+CDV+DSKGAYLR++LSLLFI
Sbjct: 361 IEHDVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Sed0006822.1 vs. ExPASy TrEMBL
Match: A0A0A0M0C2 (DDE Tnp4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G481730 PE=3 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.8e-194
Identity = 336/391 (85.93%), Postives = 354/391 (90.54%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRGLRKKKK E KLD N GTASDSSEKDDA+DWWDDFS+R NGL S  KG DRFKSI
Sbjct: 1   MGPIRGLRKKKKLERKLDCN-GTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKS +FTFLNGRPLSL DQVAVALRRLGSGESLV IGDS 
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESMEERGL HLHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEERGLHHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLPASDP+S VWLD +KNHSMVLQVIVDAEMRFRDILTG PGK+SD LVFQSS F KL
Sbjct: 181 TMCLPASDPTSYVWLDDKKNHSMVLQVIVDAEMRFRDILTGLPGKLSDWLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           C+KGERLNGK  EL D SEIREY++GDSGYPLLPYLVTPYDGKEL  SK EFNKRH ETR
Sbjct: 241 CDKGERLNGKRFELPDRSEIREYIIGDSGYPLLPYLVTPYDGKELSTSKTEFNKRHKETR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQRALA LKERWRII GVMWRPDKHRLPRIILVCCLLHNI IDIGDE ++G +PL  S
Sbjct: 301 LVVQRALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEEGEVPL--S 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHD DYKQQ+CDV+DSKGAY+RD+LSLLFI
Sbjct: 361 IEHDVDYKQQVCDVFDSKGAYVRDRLSLLFI 388

BLAST of Sed0006822.1 vs. ExPASy TrEMBL
Match: A0A5A7SXL0 (Putative nuclease HARBI1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43059G001000 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.9e-194
Identity = 337/391 (86.19%), Postives = 356/391 (91.05%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEKDDALDWWDDFSRRINGLSSPLKGSDRFKSI 60
           MGPIRGLRKKKK E KLD+N GTASDSSEKDDA+DWWDDFS+R NGL S  KG DRFKSI
Sbjct: 1   MGPIRGLRKKKKLERKLDSN-GTASDSSEKDDAIDWWDDFSKRTNGLHSASKGLDRFKSI 60

Query: 61  FKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRRLGSGESLVMIGDSF 120
           FKVSRKTFDYICLLVKDDMTAKS +FTFLNGRPLSL DQVAVALRRLGSGESLV IGDS 
Sbjct: 61  FKVSRKTFDYICLLVKDDMTAKSGHFTFLNGRPLSLCDQVAVALRRLGSGESLVTIGDSL 120

Query: 121 GVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180
           G+NHSTVSQVTWRFVESMEERGL HLHWPS EVEMAQVKSKFEKIQGLPNCCGSIDTTHI
Sbjct: 121 GLNHSTVSQVTWRFVESMEERGLCHLHWPSNEVEMAQVKSKFEKIQGLPNCCGSIDTTHI 180

Query: 181 TMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKL 240
           TMCLPASDP+S VWLD EKNHSMVLQVIVDAEMRFRDILTG PGK+SD LVFQSS F KL
Sbjct: 181 TMCLPASDPTSFVWLDDEKNHSMVLQVIVDAEMRFRDILTGLPGKLSDRLVFQSSNFHKL 240

Query: 241 CEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETR 300
           C+KGERLNGK LEL D SEI+EY+VGDSGYPLL YLVTPYDGKEL  SKAEFNKRH  TR
Sbjct: 241 CDKGERLNGKRLELPDRSEIQEYIVGDSGYPLLSYLVTPYDGKELSTSKAEFNKRHTATR 300

Query: 301 VVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDEKDDGNIPLSMS 360
           +VVQ+ALA LKERWRII GVMWRPDKHRLPRIILVCCLLHNI IDIGDE +DG++PL  S
Sbjct: 301 LVVQQALAMLKERWRIIQGVMWRPDKHRLPRIILVCCLLHNIIIDIGDETEDGDVPL--S 360

Query: 361 IEHDADYKQQICDVYDSKGAYLRDKLSLLFI 392
           IEHD DYKQQ+CDV+DSKGAYLR++LSLLFI
Sbjct: 361 IEHDVDYKQQVCDVFDSKGAYLRERLSLLFI 388

BLAST of Sed0006822.1 vs. TAIR 10
Match: AT3G55350.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 459.5 bits (1181), Expect = 2.6e-129
Identity = 230/405 (56.79%), Postives = 296/405 (73.09%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLD-------------ANGGTASDSSEKDD-----ALDWWDDFSR 60
           MGPI+ ++KKK+ E K+D             A+   A ++++ DD     +LDWWD FSR
Sbjct: 1   MGPIKTIKKKKRAEKKVDRNVLLAATAAATSASAAAALNNNDDDDDSSSQSLDWWDGFSR 60

Query: 61  RINGLSSPLKGSDRFKSIFKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAV 120
           RI G S+  K    F+S+FK+SRKTFDYIC LVK D TAK +NF+  NG PLSL D+VAV
Sbjct: 61  RIYGGSTDPK---TFESVFKISRKTFDYICSLVKADFTAKPANFSDSNGNPLSLNDRVAV 120

Query: 121 ALRRLGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKF 180
           ALRRLGSGESL +IG++FG+N STVSQ+TWRFVESMEER + HL WPS   ++ ++KSKF
Sbjct: 121 ALRRLGSGESLSVIGETFGMNQSTVSQITWRFVESMEERAIHHLSWPS---KLDEIKSKF 180

Query: 181 EKIQGLPNCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGF 240
           EKI GLPNCCG+ID THI M LPA +PS+ VWLD EKN SM LQ +VD +MRF D++ G+
Sbjct: 181 EKISGLPNCCGAIDITHIVMNLPAVEPSNKVWLDGEKNFSMTLQAVVDPDMRFLDVIAGW 240

Query: 241 PGKMSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDG 300
           PG ++D +V ++S F KL EKG+RLNG+ L L + +E+REY+VGDSG+PLLP+L+TPY G
Sbjct: 241 PGSLNDDVVLKNSGFYKLVEKGKRLNGEKLPLSERTELREYIVGDSGFPLLPWLLTPYQG 300

Query: 301 KELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNI 360
           K     + EFNKRH+E     Q AL+ LK+RWRII+GVMW PD++RLPRII VCCLLHNI
Sbjct: 301 KPTSLPQTEFNKRHSEATKAAQMALSKLKDRWRIINGVMWMPDRNRLPRIIFVCCLLHNI 360

Query: 361 AIDIGDEKDDGNIPLSMSIEHDADYKQQICDVYDSKGAYLRDKLS 388
            ID+ D+  D   PLS   +HD +Y+Q+ C + D   + LRD+LS
Sbjct: 361 IIDMEDQTLDDQ-PLSQ--QHDMNYRQRSCKLADEASSVLRDELS 396

BLAST of Sed0006822.1 vs. TAIR 10
Match: AT3G63270.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: PIF / Ping-Pong family of plant transposases (TAIR:AT3G55350.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 341.3 bits (874), Expect = 1.0e-93
Identity = 185/398 (46.48%), Postives = 248/398 (62.31%), Query Frame = 0

Query: 1   MGPIRGLRKKKKFEAKLDANGGTASDSSEK--------DDAL--DWWDDFSRRINGLSSP 60
           M P++  +KKK  +  LD     A +  +K         +A+  DWWD F  R +  S P
Sbjct: 1   MAPVK--QKKKNKKKPLDKAKKLAKNKEKKRVNAVPLDPEAIDCDWWDTFWLRNSSPSVP 60

Query: 61  LKGSDRFKSIFKVSRKTFDYICLLVKDDMTAK-SSNFTFLNGRPLSLYDQVAVALRRLGS 120
                 FK  F+ S+ TF YIC LV++D+ ++  S    + GR LS+  QVA+ALRRL S
Sbjct: 61  SDEDYAFKHFFRASKTTFSYICSLVREDLISRPPSGLINIEGRLLSVEKQVAIALRRLAS 120

Query: 121 GESLVMIGDSFGVNHSTVSQVTWRFVESMEERGLRHLHWPSTEVEMAQVKSKFEKIQGLP 180
           G+S V +G +FGV  STVSQVTWRF+E++EER   HL WP ++  + ++KSKFE++ GLP
Sbjct: 121 GDSQVSVGAAFGVGQSTVSQVTWRFIEALEERAKHHLRWPDSD-RIEEIKSKFEEMYGLP 180

Query: 181 NCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGKMSDS 240
           NCCG+IDTTHI M LPA   S + W DQEKN+SM LQ + D EMRF +++TG+PG M+ S
Sbjct: 181 NCCGAIDTTHIIMTLPAVQASDD-WCDQEKNYSMFLQGVFDHEMRFLNMVTGWPGGMTVS 240

Query: 241 LVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPYDGKELQPSK 300
            + + S F KLCE  + L+G    L   ++IREYVVG   YPLLP+L+TP+D      S 
Sbjct: 241 KLLKFSGFFKLCENAQILDGNPKTLSQGAQIREYVVGGISYPLLPWLITPHDSDHPSDSM 300

Query: 301 AEFNKRHAETRVVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNIAIDIGDE 360
             FN+RH + R V   A   LK  WRI+  VMWRPD+ +LP IILVCCLLHNI ID GD 
Sbjct: 301 VAFNERHEKVRSVAATAFQQLKGSWRILSKVMWRPDRRKLPSIILVCCLLHNIIIDCGDY 360

Query: 361 KDDGNIPLSMSIEHDADYKQQICDVYDSKGAYLRDKLS 388
             + ++PLS    HD+ Y  + C   +  G+ LR  L+
Sbjct: 361 LQE-DVPLSG--HHDSGYADRYCKQTEPLGSELRGCLT 391

BLAST of Sed0006822.1 vs. TAIR 10
Match: AT5G12010.1 (unknown protein; INVOLVED IN: response to salt stress; LOCATED IN: chloroplast, plasma membrane, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G29780.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 145.6 bits (366), Expect = 8.4e-35
Identity = 91/327 (27.83%), Postives = 163/327 (49.85%), Query Frame = 0

Query: 30  KDDALDWWDDFSRRINGLSSPLKGSDRFKSIFKVSRKTFDYICLLVKDDMTAKSSNFTFL 89
           KD +  WW++ SR    L  P    + FK  F++S+ TF+ IC    D++ +  +     
Sbjct: 155 KDRSRAWWEECSR----LDYP---EEDFKKAFRMSKSTFELIC----DELNSAVAKEDTA 214

Query: 90  NGRPLSLYDQVAVALRRLGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGL-RHLHW 149
               + +  +VAV + RL +GE L ++   FG+  ST  ++     +++++  + ++L W
Sbjct: 215 LRNAIPVRQRVAVCIWRLATGEPLRLVSKKFGLGISTCHKLVLEVCKAIKDVLMPKYLQW 274

Query: 150 PSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLPASDPSS-----NVWLDQEKNHSM 209
           P  E  +  ++ +FE + G+PN  GS+ TTHI +  P    +S     +   +Q+ ++S+
Sbjct: 275 PDDE-SLRNIRERFESVSGIPNVVGSMYTTHIPIIAPKISVASYFNKRHTERNQKTSYSI 334

Query: 210 VLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREY 269
            +Q +V+ +  F D+  G+PG M D  V + S   +    G  L G W            
Sbjct: 335 TIQAVVNPKGVFTDLCIGWPGSMPDDKVLEKSLLYQRANNGGLLKGMW------------ 394

Query: 270 VVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHGVMWR 329
           V G  G+PLL +++ PY  + L  ++  FN++ +E + V + A   LK RW  +      
Sbjct: 395 VAGGPGHPLLDWVLVPYTQQNLTWTQHAFNEKMSEVQGVAKEAFGRLKGRWACLQ-KRTE 454

Query: 330 PDKHRLPRIILVCCLLHNIAIDIGDEK 351
                LP ++  CC+LHNI  ++ +EK
Sbjct: 455 VKLQDLPTVLGACCVLHNIC-EMREEK 455

BLAST of Sed0006822.1 vs. TAIR 10
Match: AT4G29780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12010.1); Has 945 Blast hits to 944 proteins in 87 species: Archae - 0; Bacteria - 0; Metazoa - 519; Fungi - 43; Plants - 365; Viruses - 0; Other Eukaryotes - 18 (source: NCBI BLink). )

HSP 1 Score: 132.5 bits (332), Expect = 7.4e-31
Identity = 90/319 (28.21%), Postives = 151/319 (47.34%), Query Frame = 0

Query: 30  KDDALDWWDDFSRRINGLSSPLKGSDRFKSIFKVSRKTFDYICLLVKDDMTAKSSNFTFL 89
           K+   DWWD  SR       P    D F+  F++S+ TF+ IC  +   +T K++     
Sbjct: 193 KERTTDWWDRVSR-------PDFPEDEFRREFRMSKSTFNLICEELDTTVTKKNTMLRDA 252

Query: 90  NGRPLSLYDQVAVALRRLGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGL-RHLHW 149
              P     +V V + RL +G  L  + + FG+  ST  ++      ++ +  + ++L W
Sbjct: 253 IPAP----KRVGVCVWRLATGAPLRHVSERFGLGISTCHKLVIEVCRAIYDVLMPKYLLW 312

Query: 150 PSTEVEMAQVKSKFEKIQGLPNCCGSIDTTHITMCLP-----ASDPSSNVWLDQEKNHSM 209
           PS + E+   K+KFE +  +PN  GSI TTHI +  P     A     +   +Q+ ++S+
Sbjct: 313 PS-DSEINSTKAKFESVHKIPNVVGSIYTTHIPIIAPKVHVAAYFNKRHTERNQKTSYSI 372

Query: 210 VLQVIVDAEMRFRDILTGFPGKMSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREY 269
            +Q +V+A+  F D+  G PG ++D  + + S+  +       L   W            
Sbjct: 373 TVQGVVNADGIFTDVCIGNPGSLTDDQILEKSSLSRQRAARGMLRDSW------------ 432

Query: 270 VVGDSGYPLLPYLVTPYDGKELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHGVMWR 329
           +VG+SG+PL  YL+ PY  + L  ++  FN+   E + +   A   LK RW  +      
Sbjct: 433 IVGNSGFPLTDYLLVPYTRQNLTWTQHAFNESIGEIQGIATAAFERLKGRWACLQ-KRTE 486

Query: 330 PDKHRLPRIILVCCLLHNI 343
                LP ++  CC+LHNI
Sbjct: 493 VKLQDLPYVLGACCVLHNI 486

BLAST of Sed0006822.1 vs. TAIR 10
Match: AT3G19120.1 (PIF / Ping-Pong family of plant transposases )

HSP 1 Score: 105.5 bits (262), Expect = 9.7e-23
Identity = 80/299 (26.76%), Postives = 143/299 (47.83%), Query Frame = 0

Query: 47  LSSPLKGSDRFKSIFKVSRKTFDYICLLVKDDMTAKSSNFTFLNGRPLSLYDQVAVALRR 106
           L +PL+ + R++S++ +S   F  +   +K  +TA  SN +      L     VA+ L R
Sbjct: 108 LDAPLRDA-RWRSLYGLSYPVFITVVDKLKPFITA--SNLS------LPADYAVAMVLSR 167

Query: 107 LGSGESLVMIGDSFGVNHSTVSQVTWRFVESMEERGL-RHLHWPSTEVEMAQVKSKFEKI 166
           L  G S   +   + ++   +S++T      +  +     +  P  +  + +    FE++
Sbjct: 168 LAHGCSAKTLASRYSLDPYLISKITNMVTRLLATKLYPEFIKIPVGKRRLIETTQGFEEL 227

Query: 167 QGLPNCCGSIDTTHITMCLPASDPSSNVWLDQEKNHSMVLQVIVDAEMRFRDILTGFPGK 226
             LPN CG+ID+T + +         N++  +    +++LQV+ D +  F D+    PG 
Sbjct: 228 TSLPNICGAIDSTPVKLRRRTKLNPRNIYGCKYGYDAVLLQVVADHKKIFWDVCVKAPGG 287

Query: 227 MSDSLVFQSSTFQKLCEKGERLNGKWLELVDSSEIREYVVGDSGYPLLPYLVTPY--DGK 286
             DS  F+ S   K    G+ +  K +  +    +R Y+VGD  YPLL +L+TP+  +G 
Sbjct: 288 EDDSSHFRDSLLYKRLTSGDIVWEKVIN-IRGHHVRPYIVGDWCYPLLSFLMTPFSPNGS 347

Query: 287 ELQPSKAEFNKRHAETRVVVQRALASLKERWRIIHGVMWRPDKHRLPRIILVCCLLHNI 343
              P    F+    + R VV  A+  LK RW+I+  +      +  P+ I+ CC+LHN+
Sbjct: 348 GTPPENL-FDGMLMKGRSVVVEAIGLLKARWKILQSL--NVGVNHAPQTIVACCVLHNL 393