Sed0006346.2 (mRNA) Chayote v1

Overview


Name	Sed0006346.2
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	tRNA pseudouridine synthase
Location	LG09: 6686082 .. 6690795 (-)
Sequence length	1258
RNA-Seq Expression	Sed0006346.2
Synteny	Sed0006346.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCCTAAGCAAAGATCCATTTTGAATGTTGAATTGAAACAAAAGACTTGGAAGAGAAGGATGAGGAACTGGGTAGCGGGTTCAACAGTTGTTCCTTCCAATCCCATTTCACCGACCTTCACATCTCCAAATCGCTCATCATCTTCCACAGCTCGCAGATTCTCGGTAATGCCCAATTCTTCTCTAATCTCTCCTCATATTTTCATGGCTCAGTTTTCACCAATCCCATATTGATTCTTCAGAAGAATGCACAGGATTCTTGTTATGTTGCAGAGCCTTATTCCCCTGGCTTTAAATGGCGGTTGCTCATTGCTTATGATGGTACTCCATATGCAGGTCCGGGTTTCTTTTTTGTGGCGAATGTATTCAACCATTTTGTATGCTTGTTCTTGGTTTAGCCTTATATGACGACTCTAATTCCTAATTGGATTAAGCTTTAGTCTGCAACGAGTGTGACAGTACTCGAAAGCTTGGGCTTTGAAGGTGTGCTTGCCTCAAAGTCTCAGTTCGAGTCTTACATTAATTTAAAGATACCTGATACTTCCCGTGTCTCTCTCTTTTTCGATTTCTCCCGATATCTGGCCTAGGGACGGGCTTTGAATGTGTGCTCCCCTTAAAGTTTCAAGTTCCAAACTCACCTGTGACATTAATTTGTAGACACCTCTCATGTCTCCATTCTTTTGATGTTTCCCGCTCCCTGACCCTAGGAAGCACGAACACATCTATTTTGACAATGTATCCGAGTTGTACTCGTGTAAAACCAGTGTTTACAAGGCCATCACCTTTCCTCGCCTATGGCCATCGCCTTGCACATAGGCAGGTATTGCGCCTCTAACATAGCTAAGGCGAGCTCCTTCAATCAGGCCCTCGCATTTCCTCGCCATTTTGCGCTTTGCATCGCCTTCGCGTTAGGCCAAGGCGTGCGCCTTGTTAAAGTTTTATTTATTTATTAATATCATAACTTAATATCCACAAAAAGAACCTATAATTCGTTTATGAAGAAGTTCAACAACTATGTGAAGTCTTTATTAAGAATATTTAATTTTTTAATTGCTATTTCAAATATATGTGTGTTTGTTTATTAGAGTTATTTGATGTAAAATCTTTGTACGTTTAGTTAACTTCAATTTTTTTTTATACAAATAATAATTTAATATTATGGTATATCTTATAATAACATAATTTTTGTGATTTATTATTCTTTTATTTTTTACTTTTTAGTATTTTTCCATATGAATTTATAGTTTTATGAGAATAAACATATGTTTTATTATCAATTTATAAATGCGCCTCTCTTCACTCGGGTCTTGCCTTTTTATCGCCTCTCGCTTTGCTTTGAGGTTATCAAGGAGAGTATCGCCTTGTGTTGTGCCTTGCGCTTTGAAAACACTATGTCCAACACAGATATTTCCGGACAATCAACGGACACGTGTCCGACACTCAAAATTTTGTGTTTTCTTTTATTATTATTATTTTCAATTTCAGACACTTCATGGACACCTCAATTAAAACTTATTTGTTTCTAATGGCGGATGACACGACAATGCGTAGTGTTAGTGGAGGCCGTCAATGACGATGTCTATCGAAGAAAGATGAACTAAAATGAAAAAAGGAAGGAAAGAGAGAAAATAAAATGGAGGGAAGGAAAATGAGATAAAAACTAAAATATTTAGATATTATTATGGGTAGGTTTATAACTAGCGAAAATATTTTGGTTTTCATATTTTAATAGTATATTATATAAAATAATAATAAATCACGTGTCCTCGACGTATCTGTGTTCTGCATTTTTAAATAATGGCGTGTCTACGTGTCCGTATAGTGTCGTGTCCGTATCTTGTGTCCGTGCTTCTTAGTCTTGACCTAGGGACGGACATGGTTATCCCGAGTATAGTAGAGCGAAGCTCCGATTTTCCAGTTAAAAAACTGTATATATGTTTGCAGTAGAGAAAGCCACCCTGAGTAACTAAATAAGGGCTATAGATATAATAGATTATGGTTGGGTTAACCTAGTGTAAATAGAGAATTAAGAAAACAAGATGGAATGGATTTAATCTATAATAGAAAGTTAGCTAAGATACATCTTTTTTCTGTTTGACGATTGAGTTGTGAGATTAGTTGAAGTAAGCTGACTCGGACACTATTAATAAAAATGAGGAATTTGAACCCATAACCTTTTAGTTTGTGGGCACATTTATGCCTGCTGAGCTACTGGGGGGTTTAGAAGAAATATGTTCATTCCTTCGATTTAATGCAATCTTATGACTCTTATTCGCCATTTCAATTGCTATGGGAATACATACTTCTTCCCTTTAGCATTTATATGCTATTTAATTGCATGGATCCCATATCATTCTTTAATGATTCATAAATTATCGACGAACCCAAAAGTTTAAGTTGATAGGTTATGATAAATTTAATTATATCAACCAACACTCTTCTCTACTTGTGGACTTCAAAATTATGTCAATCATGTGGAAATTAACATTAATTAAGGAGAAAACGACTTCAAAGGAGTGTGAATATATGATCTCCCCTATGATTCCATGTTAAATCATGTTAAATTATGTTAAATTACGTCTCCAAGGGGTGGCACAGTGGTTGAAGACTTGGAATTTGAGGGTATGCTTCTCTTGAAGTCCCAGGTTCGAAACTTACCTATGACATTACGTGGTTACCTTATTTCAAAAAAAAAATTATATTAAATTTCCACTAATCCCAAAAGCTTAAGTTGATATGTAGGAGTAAATTTAATTATATCAACTAACATAACAGACAATAAATGGAAAATTTTCATACTATCTAGGACTAGAAAATCTCCCATTGATTTAGCTAGCTTTAAGTTATCTTAAAGATACGTGAATTGGGGTCCGGTCACCGGTGTGAATTATGAGTTAGAGAAGTTTTTTCCATTAGTTTGAATTCCTGCGCTCACTACTTCAGTTTTGATGCTCTCTGTCTTTGTTTCAAATATGTTGATATTAAATCAGGCTGGCAATATCAGCAGTCACCGCCTACCATCCAGTGCCTGATTGAAAAAGCCTTGACTCAAGCAACAAAGGTTGAAAGGAAGGATCTCCGTTTAGTTGGTGCGAGCCGAACAGATACAGGAGTTCATGCCTGGGGACAGGTCTGATGTGCTTTTATGGAGTTGGCTTAAACACATTGTTGGAAAACAATGCTTGGTGTTGCTTACAACAAGTTGAGGTTTCAGGTGGCACACTTTGTTACGCCATTTAGCTTCGATAGCTTGGAAACGGTTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATTAGAGTTCGGGAGATTAGTCCTGCTGTTCCCGACTTTCATGCGCGATTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGACACCATCTTGGATCCATTTCAGCGCTTCTACGCTTACCAATGCCTTTATAAACTCAATTCTGCAGTCATGAGAGAAGCTGCGAGTTATTTCATTGGAAAGCATGATTTTTCGGCTTTTGCGAATGCAGCGCAGAATGATCGAGTGGGAAATCCAGTGAAGGTCATTTTTCGGTTTGATGTCATAGAAATGGTAAAGTTTTTAGGTTCTATCTGTCAATGGTTTTTTTTTTCTTTCTATTCGGCTTCTCACGAGTTTAATATGGCTAGTCATGTGTTAATCATTCTGTTTTCAGCTTTATACAAAAACTATGTGGTTAAATGCTTAGCATTCGGTTGTTTTCTTTGTTCAGGGACCTCTAATTCAGCTAGAAGTTGAGGGCTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTAATTATATTTGTTACACTTTTTTTTAGTCCAAGTTATTTTGTCCGACTTTACTTGGTAAATTCTTGTTGCCACAATGACTTGTATTATGAAATAATTGTAAACAACGACCAATAATAAAGAAAGTGTGAGACAAAGAACACCTCGATTTATGTGGTTCACTAATATTGTATTAGGCCAGCTACATTCACGAGTAGAGGAAAACAACTCTTTTATTTAGGAGATTGTAGGTAAAGAGGATAAAGATTTTATCCAAATTACATAAAAGCCTAATACGAAAATATAAAAACATAGTTAAAATATTTTCTAGGTTTCGGGGTGTTGAACCTTCATCGTGTGCTTCACCTTGGACCCCTCGTAATGTGACATAATTAGTTCAAGGCATTTCGAGAGCTTGTTTTTTTTATAATAAGCTTAGATTCAATTTTCTTAAGTCGGGTTTCATCGTGCAACTGTAAGCATTTCTCCATTACTTTTGCAGGTTGGCTTGTTGATTCAAATTGGAAAGGAAGCAATTTCTCCTGATGTTGTTCCTCAGATTTTGGCTTCTCGAGATCGCAAGGAGCTCGCCAAGTACGCTCCCTTCATTCCCCCTCACGGGCTCTGTCTTGTGAGCATTAAATATAACGAGGACCATCTAAAGCTTCCATCTGGTAGCCCTGGGACTAGTTCTGGTAGGTATCATACTGTGAGCAAATGTAAACTACCTTTTTATTGAATTCATGACAGTTTACAGGTCTGTTCTTACACTTTTGCCAATGACCATGAGCATAGTTTCCTGAGGATGAAATATTAGATTGCTAGTATGATTTAGTTAATTTTGGATTAGTTAGTTTGTAATATATATGAATTAGTATTTTAGTGTTAGGTTGTTATTTGTAGTTTTAATATTGCATAACTTGTATTGAGACTTTG

mRNA sequence

GCCCTAAGCAAAGATCCATTTTGAATGTTGAATTGAAACAAAAGACTTGGAAGAGAAGGATGAGGAACTGGGTAGCGGGTTCAACAGTTGTTCCTTCCAATCCCATTTCACCGACCTTCACATCTCCAAATCGCTCATCATCTTCCACAGCTCGCAGATTCTCGAAGAATGCACAGGATTCTTGTTATGTTGCAGAGCCTTATTCCCCTGGCTTTAAATGGCGGTTGCTCATTGCTTATGATGGTACTCCATATGCAGGCTGGCAATATCAGCAGTCACCGCCTACCATCCAGTGCCTGATTGAAAAAGCCTTGACTCAAGCAACAAAGGTTGAAAGGAAGGATCTCCGTTTAGTTGGTGCGAGCCGAACAGATACAGGAGTTCATGCCTGGGGACAGGTGGCACACTTTGTTACGCCATTTAGCTTCGATAGCTTGGAAACGGTTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATTAGAGTTCGGGAGATTAGTCCTGCTGTTCCCGACTTTCATGCGCGATTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGACACCATCTTGGATCCATTTCAGCGCTTCTACGCTTACCAATGCCTTTATAAACTCAATTCTGCAGTCATGAGAGAAGCTGCGAGTTATTTCATTGGAAAGCATGATTTTTCGGCTTTTGCGAATGCAGCGCAGAATGATCGAGTGGGAAATCCAGTGAAGGTCATTTTTCGGTTTGATGTCATAGAAATGGGACCTCTAATTCAGCTAGAAGTTGAGGGCTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGGCTTGTTGATTCAAATTGGAAAGGAAGCAATTTCTCCTGATGTTGTTCCTCAGATTTTGGCTTCTCGAGATCGCAAGGAGCTCGCCAAGTACGCTCCCTTCATTCCCCCTCACGGGCTCTGTCTTGTGAGCATTAAATATAACGAGGACCATCTAAAGCTTCCATCTGGTAGCCCTGGGACTAGTTCTGGTAGGTATCATACTGTGAGCAAATGTAAACTACCTTTTTATTGAATTCATGACAGTTTACAGGTCTGTTCTTACACTTTTGCCAATGACCATGAGCATAGTTTCCTGAGGATGAAATATTAGATTGCTAGTATGATTTAGTTAATTTTGGATTAGTTAGTTTGTAATATATATGAATTAGTATTTTAGTGTTAGGTTGTTATTTGTAGTTTTAATATTGCATAACTTGTATTGAGACTTTG

Coding sequence (CDS)

ATGAGGAACTGGGTAGCGGGTTCAACAGTTGTTCCTTCCAATCCCATTTCACCGACCTTCACATCTCCAAATCGCTCATCATCTTCCACAGCTCGCAGATTCTCGAAGAATGCACAGGATTCTTGTTATGTTGCAGAGCCTTATTCCCCTGGCTTTAAATGGCGGTTGCTCATTGCTTATGATGGTACTCCATATGCAGGCTGGCAATATCAGCAGTCACCGCCTACCATCCAGTGCCTGATTGAAAAAGCCTTGACTCAAGCAACAAAGGTTGAAAGGAAGGATCTCCGTTTAGTTGGTGCGAGCCGAACAGATACAGGAGTTCATGCCTGGGGACAGGTGGCACACTTTGTTACGCCATTTAGCTTCGATAGCTTGGAAACGGTTCATGCTGCTCTGAATGGTCTTCTTCCTGCGGATATTAGAGTTCGGGAGATTAGTCCTGCTGTTCCCGACTTTCATGCGCGATTTTCTACAATAAGCAAGGTTTATCACTACAAGATATATAATGACACCATCTTGGATCCATTTCAGCGCTTCTACGCTTACCAATGCCTTTATAAACTCAATTCTGCAGTCATGAGAGAAGCTGCGAGTTATTTCATTGGAAAGCATGATTTTTCGGCTTTTGCGAATGCAGCGCAGAATGATCGAGTGGGAAATCCAGTGAAGGTCATTTTTCGGTTTGATGTCATAGAAATGGGACCTCTAATTCAGCTAGAAGTTGAGGGCTCTGGGTTCTTGTATAGACAAGTTCGGAATATGGTTGGCTTGTTGATTCAAATTGGAAAGGAAGCAATTTCTCCTGATGTTGTTCCTCAGATTTTGGCTTCTCGAGATCGCAAGGAGCTCGCCAAGTACGCTCCCTTCATTCCCCCTCACGGGCTCTGTCTTGTGAGCATTAAATATAACGAGGACCATCTAAAGCTTCCATCTGGTAGCCCTGGGACTAGTTCTGGTAGGTATCATACTGTGAGCAAATGTAAACTACCTTTTTATTGA

Protein sequence

MRNWVAGSTVVPSNPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLIAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCLVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY

Homology

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BLAST of Sed0006346.2 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 528.5 bits (1360), Expect = 4.2e-146
Identity = 258/335 (77.01%), Postives = 286/335 (85.37%), Query Frame = 0


Query: 1   MRNWVAGSTVVP--SNPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           MR W  GS V+P  S P SP   SPN +SSS++R F  NA+DS Y+ EP  P FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGF-PNAEDSSYITEPPFPSFKWRLLI 60

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCLIE+AL  ATK+ERKDL LVGASRTD GVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSLE+VHAALNGLLP DIRVRE+SPA PDFHARFST+SKVYHYKIYN TI+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAF NAA N+RV NPVKVIFRFDVIEMG L+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMVGLL+QIGKEA+ P+VVP+ILASRDRKELAKYA  +PP GLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           VSI Y EDHLKLPS  PGTS GR  +V KCK+PFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

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BLAST of Sed0006346.2 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 527.3 bits (1357), Expect = 9.4e-146
Identity = 256/335 (76.42%), Postives = 286/335 (85.37%), Query Frame = 0


Query: 1   MRNWVAGSTVVP--SNPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           MR W  GS V+P  S P+SP   SPN +SSS++R F     D+ Y+ EP  P FKWRLLI
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCLIE+AL  ATK+ERKDL LVGASRTD GVHAWGQVAHFV
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSLE+VHAALNGLLP DIRVRE+SPAVPDFHARFST+SKVYHYKIY+ TI+DPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAF NAA NDRV NPVKVIFRFDVIEMG L+
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMVGLL+QIGKEA+ P+VVP+ILASRDR+ELAKYA  +PP GLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           VSI Y EDHLKLPS  PGTS GR  +VSKCK+PFY
Sbjct: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

[Close]

BLAST of Sed0006346.2 vs. NCBI nr
Match: XP_023549337.1 (uncharacterized protein LOC111807720 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 526.2 bits (1354), Expect = 2.1e-145
Identity = 259/335 (77.31%), Postives = 283/335 (84.48%), Query Frame = 0


Query: 1   MRNWVAGSTVVPS--NPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           M  W  GS V+PS   P+SP   S N  S ST R F  +   SC V EP  P FKWRL++
Sbjct: 1   MSCWAVGSAVLPSVPRPLSP-IPSRNGDSPSTGRGFPISEDSSC-VTEPDFPSFKWRLVV 60

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCLIE+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFI 120

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSL+ +HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQ
Sbjct: 121 TPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAFANAA NDRV NPVKV+FRFDVIEMG LI
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGALI 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEAI PDVVP+ILA+RDRKELAKY   IPPHGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLCL 300

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           V I Y EDHLKLPS  PGTS GR H+VSKCKLPFY
Sbjct: 301 VRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

[Close]

BLAST of Sed0006346.2 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 525.8 bits (1353), Expect = 2.7e-145
Identity = 258/335 (77.01%), Postives = 285/335 (85.07%), Query Frame = 0


Query: 1   MRNWVAGSTVVP--SNPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           MR W  GS V+P  S P+SP    PN +SSS++R  S NA+DS  +  P  P FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRG-SPNAEDSSCIIAPPFPSFKWRLLI 60

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCLIE+AL  ATK+ERKDL LVGASRTD GVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSLE+VHAALNGLLP DIRVREISPA PDFHARFST+SKVYHYKIYN  ILDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAF NAA NDRV NPVKVIFRFDVI+MGPL+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMVGLL+QIGKEAI PDVVP+ILAS+DR+ELAKYA  +PP GLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCL 300

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           VSI Y EDHLKLPS  PGTS GR  +VSKCKLPFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

[Close]

BLAST of Sed0006346.2 vs. NCBI nr
Match: XP_023549334.1 (uncharacterized protein LOC111807720 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.4 bits (1352), Expect = 3.6e-145
Identity = 259/336 (77.08%), Postives = 284/336 (84.52%), Query Frame = 0


Query: 1   MRNWVAGSTVVPS--NPISPTFTSPNRSSSSTARRFSKN-AQDSCYVAEPYSPGFKWRLL 60
           M  W  GS V+PS   P+SP   S N  S ST R F    ++DS  V EP  P FKWRL+
Sbjct: 1   MSCWAVGSAVLPSVPRPLSP-IPSRNGDSPSTGRGFPVTISEDSSCVTEPDFPSFKWRLV 60

Query: 61  IAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHF 120
           +AYDGT YAGWQYQ+SPPTIQCLIE+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF
Sbjct: 61  VAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHF 120

Query: 121 VTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPF 180
           +TPF++DSL+ +HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPF
Sbjct: 121 ITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPF 180

Query: 181 QRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPL 240
           QR Y Y C+YKLN A M+EAA+YF+GKHDFSAFANAA NDRV NPVKV+FRFDVIEMG L
Sbjct: 181 QRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANAAHNDRVVNPVKVVFRFDVIEMGAL 240

Query: 241 IQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLC 300
           IQLEVEGSGFLYRQVRNMV LL+QIGKEAI PDVVP+ILA+RDRKELAKY   IPPHGLC
Sbjct: 241 IQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILATRDRKELAKYTTCIPPHGLC 300

Query: 301 LVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           LV I Y EDHLKLPS  PGTS GR H+VSKCKLPFY
Sbjct: 301 LVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335

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BLAST of Sed0006346.2 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.4e-51
Identity = 105/255 (41.18%), Postives = 161/255 (63.14%), Query Frame = 0


Query: 54  WRLLIAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQ 113
           ++L IAYDGT Y+GWQ Q +  +IQ  I++AL    K E+  + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEALCILLKKEK--VVLVGSGRTDAGVHAKGQ 63

Query: 114 VAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTI 173
           VAHF      D L  +H +LNGLLP DIR++ + P  P FH+++S I K YHY ++ + +
Sbjct: 64  VAHFHFQDYID-LSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 174 LDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVG-NPVKVIFRFDVI 233
           +DPFQR Y++    K++  ++++AA  F G HDF++FAN A       NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 234 EMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIP 293
                ++LE EG GFLY+ VRN+VG L+ +     + + + QI A+++R++ +  A   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---P 243

Query: 294 PHGLCLVSIKYNEDH 308
           P GL L+ + Y  ++
Sbjct: 244 PEGLFLIQVFYENEN 252

[Close]

BLAST of Sed0006346.2 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 1.0e-49
Identity = 107/252 (42.46%), Postives = 148/252 (58.73%), Query Frame = 0


Query: 55  RLLIAYDGTPYAGWQYQQSP--PTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWG 114
           RL +AYDGT Y G+Q Q     PTIQ  +E+ L + +      L++ GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCLAELSGAA---LKVTGASRTDAGVHARG 64

Query: 115 QVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDT 174
           QV +FVT       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +YN  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 175 ILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVI 234
           +  PFQR Y+      L+   MR AA + +G+HDFS F  A +   V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRADIE 184

Query: 235 EMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIP 294
              PL++L     GFLY  VR M G L+++G E + P+ +P I+AS DR   A+  P +P
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 295 PHGLCLVSIKYN 305
           PHGLCL  ++Y+
Sbjct: 245 PHGLCLEEVEYD 248

[Close]

BLAST of Sed0006346.2 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.7e-45
Identity = 101/251 (40.24%), Postives = 149/251 (59.36%), Query Frame = 0


Query: 55  RLLIAYDGTPYAGWQYQQSP--PTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWG 114
           +L +AYDGT Y G+Q Q+     TIQ  +EKAL   + + +  ++++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQG 64

Query: 115 QVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDT 174
           QV +F +       E    ALN LLP DI+V +      DFHARFS ++K Y Y IY+  
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 175 ILDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVI 234
           ++ PF R+Y Y    +L+   M+E A+Y +G +DF +F   AQ   V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 235 EMGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIP 294
           E  P+I L + G+GFLY  VR + G L+ IG   I P+ + +I+ S++R      A   P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---P 244

Query: 295 PHGLCLVSIKY 304
           P GLCL+ ++Y
Sbjct: 245 PQGLCLMEVEY 247

[Close]

BLAST of Sed0006346.2 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.5e-45
Identity = 111/248 (44.76%), Postives = 145/248 (58.47%), Query Frame = 0


Query: 56  LLIAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVA 115
           L+I YDGT Y GWQYQ++  T+Q ++EKA+    KV  + + L+G+SRTDTGVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAI---EKVTGEKVNLIGSSRTDTGVHALYQVA 61

Query: 116 HFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILD 175
           +F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I N     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 176 PFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMG 235
           P  R Y +   Y L+   M++AA +F G HDFSAF  A+ N +V NPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF-KASGNKKV-NPVKTIFDLTVEKEG 181

Query: 236 PLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHG 295
            LI++EVE  GFLY  VR +VG L+ +G   I    +P I+ S+DR +    A   PP G
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDRTKAGITA---PPQG 240

Query: 296 LCLVSIKY 304
           L LV I Y
Sbjct: 242 LYLVRIVY 240

[Close]

BLAST of Sed0006346.2 vs. ExPASy Swiss-Prot
Match: Q9PJT0 (tRNA pseudouridine synthase A OS=Chlamydia muridarum (strain MoPn / Nigg) OX=243161 GN=truA PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 4.8e-44
Identity = 100/250 (40.00%), Postives = 144/250 (57.60%), Query Frame = 0


Query: 56  LLIAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVA 115
           L IAY GT Y+GWQYQ +  +IQ ++E   T   K+ R  + ++ + RTD GVHA GQVA
Sbjct: 7   LRIAYQGTAYSGWQYQPNALSIQEVLE---TLLQKISRTRISVIASGRTDAGVHAQGQVA 66

Query: 116 HFVTPF--SFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTI 175
           HF  P    F     +   LN LLP DI +R++     DFH+RFS I+K YHY +     
Sbjct: 67  HFCCPDHPHFSDPGQIKKMLNALLPHDIVIRDVIATNEDFHSRFSAITKEYHYTLSLLPK 126

Query: 176 LDPFQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIE 235
             P  R + +   +KL    MREAA Y +G HDF++FAN  +     + V+ ++  D++E
Sbjct: 127 PLPHHRLFCFSPRHKLCLESMREAAKYLVGTHDFASFANLGR--EYSSTVRTLYALDLLE 186

Query: 236 MGPLIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPP 295
              L+ +  +G+GFLY+ VRN+VG L+ IGK    P+ + ++L  +DRK   K  P  PP
Sbjct: 187 QEHLVTVICKGNGFLYKMVRNIVGALLDIGKGKYPPEHLLEMLEKKDRK---KGPPSAPP 246

Query: 296 HGLCLVSIKY 304
           +GL L  + Y
Sbjct: 247 YGLSLHHVCY 248

[Close]

BLAST of Sed0006346.2 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 2.0e-146
Identity = 258/335 (77.01%), Postives = 286/335 (85.37%), Query Frame = 0


Query: 1   MRNWVAGSTVVP--SNPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           MR W  GS V+P  S P SP   SPN +SSS++R F  NA+DS Y+ EP  P FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGF-PNAEDSSYITEPPFPSFKWRLLI 60

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCLIE+AL  ATK+ERKDL LVGASRTD GVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSLE+VHAALNGLLP DIRVRE+SPA PDFHARFST+SKVYHYKIYN TI+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAF NAA N+RV NPVKVIFRFDVIEMG L+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMVGLL+QIGKEA+ P+VVP+ILASRDRKELAKYA  +PP GLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           VSI Y EDHLKLPS  PGTS GR  +V KCK+PFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

[Close]

BLAST of Sed0006346.2 vs. ExPASy TrEMBL
Match: A0A6J1H621 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.9e-145
Identity = 256/335 (76.42%), Postives = 282/335 (84.18%), Query Frame = 0


Query: 1   MRNWVAGSTVVPSNP--ISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           M  W  GS V+PS P  +SP+  S N  SS T R F  +   SC V EP  P FKWRL+I
Sbjct: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPISEDSSC-VTEPDFPSFKWRLVI 60

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCL+E+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHFI 120

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSL+ +HAALNGLLPADIRVRE+SPAVPDFHARFST+SKVYHYKIYND ILDPFQ
Sbjct: 121 TPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG LI
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEAI PD+VP+ILA+RDRKELAKY   IPP GLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLCL 300

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           V I Y EDHLKLPS  PGTS GR H+VSKCKLPFY
Sbjct: 301 VRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 334

[Close]

BLAST of Sed0006346.2 vs. ExPASy TrEMBL
Match: A0A6J1H4M7 (tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 6.6e-145
Identity = 256/336 (76.19%), Postives = 283/336 (84.23%), Query Frame = 0


Query: 1   MRNWVAGSTVVPSNP--ISPTFTSPNRSSSSTARRFSKN-AQDSCYVAEPYSPGFKWRLL 60
           M  W  GS V+PS P  +SP+  S N  SS T R F    ++DS  V EP  P FKWRL+
Sbjct: 1   MSYWAVGSAVLPSVPRLLSPSIPSRNGDSSPTGRGFPVTISEDSSCVTEPDFPSFKWRLV 60

Query: 61  IAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHF 120
           IAYDGT YAGWQYQ+SPPTIQCL+E+ALT AT++ERKDL LVGASRTDTGVHAWGQVAHF
Sbjct: 61  IAYDGTRYAGWQYQRSPPTIQCLVEEALTGATRLERKDLHLVGASRTDTGVHAWGQVAHF 120

Query: 121 VTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPF 180
           +TPF++DSL+ +HAALNGLLPADIRVRE+SPAVPDFHARFST+SKVYHYKIYND ILDPF
Sbjct: 121 ITPFNYDSLDRIHAALNGLLPADIRVREMSPAVPDFHARFSTLSKVYHYKIYNDAILDPF 180

Query: 181 QRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPL 240
           QR Y Y C+YKLN A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG L
Sbjct: 181 QRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGAL 240

Query: 241 IQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLC 300
           IQLEVEGSGFLYRQVRNMV LL+QIGKEAI PD+VP+ILA+RDRKELAKY   IPP GLC
Sbjct: 241 IQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDIVPKILATRDRKELAKYTTCIPPQGLC 300

Query: 301 LVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           LV I Y EDHLKLPS  PGTS GR H+VSKCKLPFY
Sbjct: 301 LVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 336

[Close]

BLAST of Sed0006346.2 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.3e-144
Identity = 256/335 (76.42%), Postives = 282/335 (84.18%), Query Frame = 0


Query: 1   MRNWVAGSTVVPS--NPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLI 60
           M  W  GS V+PS   P+SP+   P+R + S+  R   N++DS  V EP  P FKWRL+I
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSI--PSRGTLSSTGRGFPNSEDSSCVPEPDFPSFKWRLVI 60

Query: 61  AYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFV 120
           AYDGT YAGWQYQ+SPPTIQCLIE+ALT AT++ERKDL LVGASRTD GVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 121 TPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQ 180
           TPF++DSL+ +HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDPFQ
Sbjct: 121 TPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180

Query: 181 RFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLI 240
           R Y Y C+YKLN A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG LI
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240

Query: 241 QLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGLCL 300
           QLEVEGSGFLYRQVRNMV LL+QIGKEAI PDVVP+ILASRDRK LAKY   IPP GLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCL 300

Query: 301 VSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           V I Y EDHLKLPS  PGTS GR H+VSKCKLPFY
Sbjct: 301 VRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

[Close]

BLAST of Sed0006346.2 vs. ExPASy TrEMBL
Match: A0A6J1L2M8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 9.5e-144
Identity = 259/337 (76.85%), Postives = 284/337 (84.27%), Query Frame = 0


Query: 1   MRNWVAGSTVVPS--NPISPTFTSPNRSS-SSTARRFS-KNAQDSCYVAEPYSPGFKWRL 60
           M  W  GS V+PS   P+SP+   P+R + SST R F   N++DS  V EP  P FKWRL
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSI--PSRGTLSSTGRGFPVMNSEDSSCVPEPDFPSFKWRL 60

Query: 61  LIAYDGTPYAGWQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAH 120
           +IAYDGT YAGWQYQ+SPPTIQCLIE+ALT AT++ERKDL LVGASRTD GVHAWGQVAH
Sbjct: 61  VIAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAH 120

Query: 121 FVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDP 180
           F+TPF++DSL+ +HAALNGLLPADIRVREISPAVPDFHARFST+SKVYHYKIYND ILDP
Sbjct: 121 FITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDP 180

Query: 181 FQRFYAYQCLYKLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGP 240
           FQR Y Y C+YKLN A M+EAA+YF+GKHDFSAFAN A NDRV NPVKVIFRFDVIEMG 
Sbjct: 181 FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGA 240

Query: 241 LIQLEVEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPFIPPHGL 300
           LIQLEVEGSGFLYRQVRNMV LL+QIGKEAI PDVVP+ILASRDRK LAKY   IPP GL
Sbjct: 241 LIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGL 300

Query: 301 CLVSIKYNEDHLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           CLV I Y EDHLKLPS  PGTS GR H+VSKCKLPFY
Sbjct: 301 CLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335

[Close]

BLAST of Sed0006346.2 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 429.1 bits (1102), Expect = 3.2e-120
Identity = 215/327 (65.75%), Postives = 256/327 (78.29%), Query Frame = 0


Query: 8   STVVPSNPISPTFTSPNRSSSSTARRFSKNAQDSCYVAEPYSPGFKWRLLIAYDGTPYAG 67
           S V P+ P S  FT  N   SS       N   S  V++  S  +KWRL+IAYDGT +AG
Sbjct: 2   SCVTPALPPS-LFT--NGYLSSPQNISMTNLNSSVKVSD--SGAYKWRLVIAYDGTRFAG 61

Query: 68  WQYQQSPPTIQCLIEKALTQATKVERKDLRLVGASRTDTGVHAWGQVAHFVTPFSFDSLE 127
           WQYQ+SPPTIQ ++EKAL Q T++ RK+L+L+GA RTD GVHAWGQVAHFVTPF++ SL+
Sbjct: 62  WQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSLD 121

Query: 128 TVHAALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLY 187
           + HAALNGLLP DIRVRE+S AVP+FHARFS  SKVY Y+IYNDT +DPFQR +AY C Y
Sbjct: 122 SFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCAY 181

Query: 188 KLNSAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGPLIQLEVEGSGF 247
           KLN++ MREAA+ F+GKHDFSAFANA + D V +P+K I RFDVI+MG L+QLEVEGSGF
Sbjct: 182 KLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSGF 241

Query: 248 LYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKYAPF-IPPHGLCLVSIKYNED 307
           LYRQVRNMV LLIQIGKEA+  D+VP IL ++DR+ LAKY      PHGLCLVS+KY ED
Sbjct: 242 LYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKED 301

Query: 308 HLKLPSGSPGTSSGRYHTVSKCKLPFY 334
           HLKLP   P TSSGR+HT++KCKLPFY
Sbjct: 302 HLKLPLDCPVTSSGRHHTITKCKLPFY 323

[Close]

BLAST of Sed0006346.2 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.7e-15
Identity = 84/286 (29.37%), Postives = 118/286 (41.26%), Query Frame = 0


Query: 32  RRFSKNAQDSCYVAEPYSPGFKWRLLIAYDGTPYAGWQYQ-QSPPTIQCLIEKAL--TQA 91
           R+  KN+  S  +++ Y       L I Y G  + G+  + Q  P+I+  + KAL  T+ 
Sbjct: 75  RKGDKNSIPSHLISKRYV-----ALKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRL 134

Query: 92  TKVERKDLRLVGASRTDTGVHAWGQV------AHFVTPFSFDSLETVHA----------- 151
              ++KD       RTD GV + GQV      +   +P   DS   V+            
Sbjct: 135 LVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSP-PGDSKAQVNGRTGERPEYDYV 194

Query: 152 -ALNGLLPADIRVREISPAVPDFHARFSTISKVYHYKIYNDTILDPFQRFYAYQCLYKLN 211
             LN  LP DIRV   SPA  DFHARFS  ++ Y Y  +                   LN
Sbjct: 195 RVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWRQ----------------NLN 254

Query: 212 SAVMREAASYFIGKHDFSAFANAAQNDRVGNPVKVIFRFDVIEMGP---------LIQLE 271
            + M  A   FIG+HDF  F        V N      R    E+ P         L    
Sbjct: 255 LSAMDFAGKKFIGEHDFRNFCKM----DVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFT 314

Query: 272 VEGSGFLYRQVRNMVGLLIQIGKEAISPDVVPQILASRDRKELAKY 288
           + GS FL+ Q+R MV +L  IG+   S DV+  +L ++      +Y
Sbjct: 315 MRGSAFLWHQIRCMVAVLFMIGQGVESVDVIDTLLDTKKTPRKPQY 334

[Close]

BLAST of Sed0006346.2 vs. TAIR 10
Match: AT5G35400.1 (Pseudouridine synthase family protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.7e-07
Identity = 39/123 (31.71%), Postives = 58/123 (47.15%), Query Frame = 0


Query: 54  WRLLIAYDGTPYAGWQYQQSPPTIQCLIEKAL--------TQATKVERKDL--RLVGASR 113
           ++++++Y+G  + GWQ Q    T+Q ++EK+L         Q  K + K L  R++ A R
Sbjct: 95  FKIVLSYNGASFDGWQKQPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGR 154

Query: 114 TDTGVHAWGQVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKV 167
           TD GV A  QV  F T         +  A+N      +RV  IS     FH  FS   + 
Sbjct: 155 TDKGVSALNQVCSFYTWRKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKWRR 214

[Close]

BLAST of Sed0006346.2 vs. TAIR 10
Match: AT5G35400.2 (Pseudouridine synthase family protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.7e-07
Identity = 39/123 (31.71%), Postives = 58/123 (47.15%), Query Frame = 0


Query: 54  WRLLIAYDGTPYAGWQYQQSPPTIQCLIEKAL--------TQATKVERKDL--RLVGASR 113
           ++++++Y+G  + GWQ Q    T+Q ++EK+L         Q  K + K L  R++ A R
Sbjct: 95  FKIVLSYNGASFDGWQKQPDLHTVQGVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGR 154

Query: 114 TDTGVHAWGQVAHFVTPFSFDSLETVHAALNGLLPADIRVREISPAVPDFHARFSTISKV 167
           TD GV A  QV  F T         +  A+N      +RV  IS     FH  FS   + 
Sbjct: 155 TDKGVSALNQVCSFYTWRKDIEPIAIEDAINKDASGKLRVVSISKVSRSFHPNFSAKWRR 214

The following BLAST results are available for this feature:

BLAST of Sed0006346.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458279.1	4.2e-146	77.01	PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo]	[more]
XP_004138518.2	9.4e-146	76.42	uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical ...	[more]
XP_023549337.1	2.1e-145	77.31	uncharacterized protein LOC111807720 isoform X4 [Cucurbita pepo subsp. pepo]	[more]
XP_038875516.1	2.7e-145	77.01	tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida]	[more]
XP_023549334.1	3.6e-145	77.08	uncharacterized protein LOC111807720 isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sed0006346.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6MEE7	1.4e-51	41.18	tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=...	[more]
B1I1B5	1.0e-49	42.46	tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477...	[more]
A4J151	5.7e-45	40.24	tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=3491...	[more]
Q8R7Y7	7.5e-45	44.76	tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongen...	[more]
Q9PJT0	4.8e-44	40.00	tRNA pseudouridine synthase A OS=Chlamydia muridarum (strain MoPn / Nigg) OX=243...	[more]

BLAST of Sed0006346.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C7L6	2.0e-146	77.01	tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1	[more]
A0A6J1H621	3.9e-145	76.42	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]
A0A6J1H4M7	6.6e-145	76.19	tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460439 PE=3 S...	[more]
A0A6J1L0L1	3.3e-144	76.42	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]
A0A6J1L2M8	9.5e-144	76.85	tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=...	[more]

BLAST of Sed0006346.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G06950.1	3.2e-120	65.75	Pseudouridine synthase family protein	[more]
AT1G34150.1	1.7e-15	29.37	Pseudouridine synthase family protein	[more]
AT5G35400.1	1.7e-07	31.71	Pseudouridine synthase family protein	[more]
AT5G35400.2	1.7e-07	31.71	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 160..301 e-value: 2.4E-38 score: 133.0
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 52..159 e-value: 5.6E-29 score: 102.5
IPR001406	Pseudouridine synthase I, TruA	TIGRFAM	TIGR00071	TIGR00071	coord: 56..289 e-value: 2.7E-56 score: 188.8
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 34..313
IPR001406	Pseudouridine synthase I, TruA	HAMAP	MF_00171	TruA	coord: 52..303 score: 32.468258
IPR001406	Pseudouridine synthase I, TruA	CDD	cd02570	PseudoU_synth_EcTruA	coord: 56..303 e-value: 4.06344E-111 score: 320.96
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 197..304 e-value: 1.3E-26 score: 93.2 coord: 59..157 e-value: 4.4E-6 score: 27.1
None	No IPR available	PANTHER	PTHR11142:SF0	TRNA PSEUDOURIDINE SYNTHASE-LIKE 1	coord: 34..313
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 53..308

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0006346	Sed0006346	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0006346.2-three_prime_utr	Sed0006346.2-three_prime_utr-LG09:6686082..6686278	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0006346.2-exon	Sed0006346.2-exon-LG09:6686082..6686515	exon
Sed0006346.2-exon	Sed0006346.2-exon-LG09:6686997..6687059	exon
Sed0006346.2-exon	Sed0006346.2-exon-LG09:6687220..6687582	exon
Sed0006346.2-exon	Sed0006346.2-exon-LG09:6687667..6687806	exon
Sed0006346.2-exon	Sed0006346.2-exon-LG09:6690461..6690554	exon
Sed0006346.2-exon	Sed0006346.2-exon-LG09:6690632..6690795	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0006346.2-cds	Sed0006346.2-cds-LG09:6686279..6686515	CDS
Sed0006346.2-cds	Sed0006346.2-cds-LG09:6686997..6687059	CDS
Sed0006346.2-cds	Sed0006346.2-cds-LG09:6687220..6687582	CDS
Sed0006346.2-cds	Sed0006346.2-cds-LG09:6687667..6687806	CDS
Sed0006346.2-cds	Sed0006346.2-cds-LG09:6690461..6690554	CDS
Sed0006346.2-cds	Sed0006346.2-cds-LG09:6690632..6690736	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0006346.2-five_prime_utr	Sed0006346.2-five_prime_utr-LG09:6690737..6690795	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0006346.2	Sed0006346.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
biological_process	GO:0034196	acylglycerol transport
biological_process	GO:1990052	ER to chloroplast lipid transport
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0070300	phosphatidic acid binding
molecular_function	GO:0106029	tRNA pseudouridine synthase activity