Sed0005487.1 (mRNA) Chayote v1

Overview
NameSed0005487.1
TypemRNA
OrganismSechium edule (Chayote v1)
DescriptionEthylene-responsive transcription factor
LocationLG04: 43782098 .. 43783387 (-)
Sequence length1290
RNA-Seq ExpressionSed0005487.1
SyntenySed0005487.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATCCCCAAATCAAAACAAACGTATTCAGATCCTACAACCACTCCGTAGCAAAAATCAATTAATTAATTCAACCGCCATGGTTGCCAAACTCAAATCCACCGCCAACAACAACAGTAATGGCCGCGATCTCCATTTCCGAGGCGTTCGAAAGAGGCCTTGGGGCCGATACGCCGCCGAGATCCGCGATCCCGGCAAGAAGACCCGCGTTTGGCTCGGCACCTTCGACACGGCGGAGGAGGCGGCGCGTGCTTACGACACCGCCGCCCGCCAGTTCCGCGGCGCCAAGGCCAAGACTAATTTCCCCCTTCCCGAACACTTGATTCACGTTTCTCCTCACCAGATCAGCCTAAGCCCCAGCCCTAGCCCTAGCCCTAGCAGCACCGTCGAATCCTCTTCTTCCTCTAGCCAGGAAAAAGCTCCCTCGCCGGAGATCCGCCGCGTCGCTCGTACTCTTCCGTTGATTCAGCCGCAGTTTTCGCATCACGGCGGCGGCCGTCCTCTTCTGTTTATGGACGGGTTTGTCCGACCGGAGTTTGTCGCTCTACGGTTTGATCGGGCGACGGTGGATTTGACGGCGAGGTTCACCGGCGAGATACAGAGAGTTGACTGCCGGCCGGCGAAGGAAACTCTGAATCTCGATCTGAATCTAGCTCCGCCGGTGGACGTTTGATTTCTCTGCTAATTTAGTTTTTTTGGATTTTTGTTTGTTAAAATTTAATTTAGTTAGGGCTTCAGAAATAGAAAAAGAAAATTAACGTGGTGTAAAGAAAAAGGGGGGAAAATTGGGCACCGTTTTTTTTTTTTTGTTAATTTGTTTTTGTGGCAGCTGAAAGAGATTGTAAGGGCTGGATCTCGCAAGTGCTGTTTGTTAAAATGGTGCAAGAAGACCGTGATCTTCGATTTTTACGTTTTGTTTTGAAGTACTTGATTTTAAAGTTGGAGCTATCTTGGAAAATAAATTTTCAAGTCTCCTAATATATATGATTGACTATCGCTTTGTTCTTATACTTATTTTACATTTAAGTTTCATTGGGTGTTTCTTTGGTGTATTTAAAGAGTTTATTGTATCCGATTCAACTATTTTAATCATTTCTTTTTTTTATCACATAGGTAGGAGTTAGCAGAGAAGTTATCGATTCTTCCACGAAAACTTACACTCAAACGAGTTTTTATTAACTCTTCCTCATTTTTATTTTAAAAATAACTCTCATTTCTAACTATTCATTTTTAATCATTCATTTATAACTATTCAATTATTTTTAACTACTTATTTTTAATCATTTCTATC

mRNA sequence

TTATCCCCAAATCAAAACAAACGTATTCAGATCCTACAACCACTCCGTAGCAAAAATCAATTAATTAATTCAACCGCCATGGTTGCCAAACTCAAATCCACCGCCAACAACAACAGTAATGGCCGCGATCTCCATTTCCGAGGCGTTCGAAAGAGGCCTTGGGGCCGATACGCCGCCGAGATCCGCGATCCCGGCAAGAAGACCCGCGTTTGGCTCGGCACCTTCGACACGGCGGAGGAGGCGGCGCGTGCTTACGACACCGCCGCCCGCCAGTTCCGCGGCGCCAAGGCCAAGACTAATTTCCCCCTTCCCGAACACTTGATTCACGTTTCTCCTCACCAGATCAGCCTAAGCCCCAGCCCTAGCCCTAGCCCTAGCAGCACCGTCGAATCCTCTTCTTCCTCTAGCCAGGAAAAAGCTCCCTCGCCGGAGATCCGCCGCGTCGCTCGTACTCTTCCGTTGATTCAGCCGCAGTTTTCGCATCACGGCGGCGGCCGTCCTCTTCTGTTTATGGACGGGTTTGTCCGACCGGAGTTTGTCGCTCTACGGTTTGATCGGGCGACGGTGGATTTGACGGCGAGGTTCACCGGCGAGATACAGAGAGTTGACTGCCGGCCGGCGAAGGAAACTCTGAATCTCGATCTGAATCTAGCTCCGCCGGTGGACGTTTGATTTCTCTGCTAATTTAGTTTTTTTGGATTTTTGTTTGTTAAAATTTAATTTAGTTAGGGCTTCAGAAATAGAAAAAGAAAATTAACGTGGTGTAAAGAAAAAGGGGGGAAAATTGGGCACCGTTTTTTTTTTTTTGTTAATTTGTTTTTGTGGCAGCTGAAAGAGATTGTAAGGGCTGGATCTCGCAAGTGCTGTTTGTTAAAATGGTGCAAGAAGACCGTGATCTTCGATTTTTACGTTTTGTTTTGAAGTACTTGATTTTAAAGTTGGAGCTATCTTGGAAAATAAATTTTCAAGTCTCCTAATATATATGATTGACTATCGCTTTGTTCTTATACTTATTTTACATTTAAGTTTCATTGGGTGTTTCTTTGGTGTATTTAAAGAGTTTATTGTATCCGATTCAACTATTTTAATCATTTCTTTTTTTTATCACATAGGTAGGAGTTAGCAGAGAAGTTATCGATTCTTCCACGAAAACTTACACTCAAACGAGTTTTTATTAACTCTTCCTCATTTTTATTTTAAAAATAACTCTCATTTCTAACTATTCATTTTTAATCATTCATTTATAACTATTCAATTATTTTTAACTACTTATTTTTAATCATTTCTATC

Coding sequence (CDS)

ATGGTTGCCAAACTCAAATCCACCGCCAACAACAACAGTAATGGCCGCGATCTCCATTTCCGAGGCGTTCGAAAGAGGCCTTGGGGCCGATACGCCGCCGAGATCCGCGATCCCGGCAAGAAGACCCGCGTTTGGCTCGGCACCTTCGACACGGCGGAGGAGGCGGCGCGTGCTTACGACACCGCCGCCCGCCAGTTCCGCGGCGCCAAGGCCAAGACTAATTTCCCCCTTCCCGAACACTTGATTCACGTTTCTCCTCACCAGATCAGCCTAAGCCCCAGCCCTAGCCCTAGCCCTAGCAGCACCGTCGAATCCTCTTCTTCCTCTAGCCAGGAAAAAGCTCCCTCGCCGGAGATCCGCCGCGTCGCTCGTACTCTTCCGTTGATTCAGCCGCAGTTTTCGCATCACGGCGGCGGCCGTCCTCTTCTGTTTATGGACGGGTTTGTCCGACCGGAGTTTGTCGCTCTACGGTTTGATCGGGCGACGGTGGATTTGACGGCGAGGTTCACCGGCGAGATACAGAGAGTTGACTGCCGGCCGGCGAAGGAAACTCTGAATCTCGATCTGAATCTAGCTCCGCCGGTGGACGTTTGA

Protein sequence

MVAKLKSTANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIRRVARTLPLIQPQFSHHGGGRPLLFMDGFVRPEFVALRFDRATVDLTARFTGEIQRVDCRPAKETLNLDLNLAPPVDV
Homology
BLAST of Sed0005487.1 vs. NCBI nr
Match: KAA0031547.1 (ethylene-responsive transcription factor 4 [Cucumis melo var. makuwa] >TYK07000.1 ethylene-responsive transcription factor 4 [Cucumis melo var. makuwa])

HSP 1 Score: 278.9 bits (712), Expect = 3.4e-71
Identity = 158/217 (72.81%), Postives = 171/217 (78.80%), Query Frame = 0

Query: 1   MVAKLKSTANN-----NSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEA 60
           MVAK KS+ NN     N+NG+DLHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEA
Sbjct: 1   MVAKEKSSPNNLPSLSNNNGKDLHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEA 60

Query: 61  ARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAP 120
           ARAYDTAARQFRGAKAKTNFP P+ LI VSPH+I    + SPSPSSTV+SSSSSS EK P
Sbjct: 61  ARAYDTAARQFRGAKAKTNFPPPDELIDVSPHKI----NQSPSPSSTVDSSSSSSHEKTP 120

Query: 121 SPEIRR---VARTLPLIQPQFSHHGGG--RPLLFMDGFVRPEFVA----LRFDRATVDLT 180
           SPEI R   V RT P IQPQF H GGG  RP+LF+D F +PEFVA    +RFD ATVDLT
Sbjct: 121 SPEISRSYGVGRTFPFIQPQFLHGGGGSVRPVLFVDVFGKPEFVAHGYPIRFDPATVDLT 180

Query: 181 ARFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
           +RFTG IQ        VD RPAKE LNLDLNLAPPVD
Sbjct: 181 SRFTGGIQSDSNSSSLVDFRPAKEILNLDLNLAPPVD 213

BLAST of Sed0005487.1 vs. NCBI nr
Match: XP_038886999.1 (ethylene-responsive transcription factor 4-like [Benincasa hispida])

HSP 1 Score: 276.6 bits (706), Expect = 1.7e-70
Identity = 157/216 (72.69%), Postives = 165/216 (76.39%), Query Frame = 0

Query: 4   KLKSTANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAA 63
           KL S +N N+N ++LHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEAARAYDTAA
Sbjct: 11  KLPSLSNTNNNVKELHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAARAYDTAA 70

Query: 64  RQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIRR-- 123
           RQFRGAKAKTNFP PE LI VSP +I    + SPSPSSTVESSSSSSQEK PSPEI R  
Sbjct: 71  RQFRGAKAKTNFPPPEELIDVSPRKI----NQSPSPSSTVESSSSSSQEKTPSPEIARSY 130

Query: 124 -VARTLPLIQPQFSHH---------GGGRPLLFMDGFVRPEFVA----LRFDRATVDLTA 183
            V RT P IQPQF H          GG RP+LFMD F RPEFVA    LRFD ATVDLTA
Sbjct: 131 GVGRTFPFIQPQFLHQVACNASGGDGGVRPVLFMDAFGRPEFVAHGYPLRFDPATVDLTA 190

Query: 184 RFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
           RF G IQ        VDCRPAKE LNLDLNLAPPVD
Sbjct: 191 RFAGGIQSDSDSSSLVDCRPAKEILNLDLNLAPPVD 222

BLAST of Sed0005487.1 vs. NCBI nr
Match: XP_008455284.2 (PREDICTED: LOW QUALITY PROTEIN: ethylene-responsive transcription factor 4 [Cucumis melo])

HSP 1 Score: 274.6 bits (701), Expect = 6.5e-70
Identity = 156/217 (71.89%), Postives = 170/217 (78.34%), Query Frame = 0

Query: 1   MVAKLKSTAN-----NNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEA 60
           MVAK KS+       +N+NG+DLHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEA
Sbjct: 1   MVAKEKSSPQQPPSLSNNNGKDLHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEA 60

Query: 61  ARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAP 120
           ARAYDTAARQFRGAKAKTNFP P+ LI VSPH+I    + SPSPSSTV+SSSSSS EK P
Sbjct: 61  ARAYDTAARQFRGAKAKTNFPPPDELIDVSPHKI----NQSPSPSSTVDSSSSSSHEKTP 120

Query: 121 SPEIRR---VARTLPLIQPQFSHHGGG--RPLLFMDGFVRPEFVA----LRFDRATVDLT 180
           SPEI R   V RT P IQPQF H GGG  RP+LF+D F +PEFVA    +RFD ATVDLT
Sbjct: 121 SPEISRSYGVGRTFPFIQPQFLHGGGGSVRPVLFVDVFGKPEFVAHGYPIRFDPATVDLT 180

Query: 181 ARFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
           +RFTG IQ        VD RPAKE LNLDLNLAPPVD
Sbjct: 181 SRFTGGIQSDSNSSSLVDFRPAKEILNLDLNLAPPVD 213

BLAST of Sed0005487.1 vs. NCBI nr
Match: XP_022989129.1 (ethylene-responsive transcription factor 4-like [Cucurbita maxima])

HSP 1 Score: 267.3 bits (682), Expect = 1.0e-67
Identity = 156/221 (70.59%), Postives = 166/221 (75.11%), Query Frame = 0

Query: 1   MVAKLKSTA-------NNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAE 60
           MV+K KSTA       N N+N R+LHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAE
Sbjct: 1   MVSKEKSTAKKLPPLCNTNNNARELHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAE 60

Query: 61  EAARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEK 120
           EAA AYD AARQFRGAKAKTNFPLP+ LI VSP +I    S SPSPSSTVESSSSSSQEK
Sbjct: 61  EAAYAYDNAARQFRGAKAKTNFPLPDELIDVSPRKI----SQSPSPSSTVESSSSSSQEK 120

Query: 121 APSPEIRR---VARTLPLIQPQFSHHGGG----RPLLFMDGFVRPEFVA----LRFDRAT 180
            PSPEI R   VART P IQPQF H  GG    RP+LFMD FVRPEFVA    +R+D   
Sbjct: 121 TPSPEIVRSYGVARTFPFIQPQFLHQVGGGGSARPVLFMDAFVRPEFVAQGYPIRYD--- 180

Query: 181 VDLTARFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
              +ARF GEIQ        VDCRPAKE LNLDLNLAPPVD
Sbjct: 181 ---SARFAGEIQTKSDSLSVVDCRPAKEILNLDLNLAPPVD 211

BLAST of Sed0005487.1 vs. NCBI nr
Match: AHB18381.1 (ethylene-responsive transcription factor [Momordica charantia])

HSP 1 Score: 266.5 bits (680), Expect = 1.8e-67
Identity = 157/232 (67.67%), Postives = 166/232 (71.55%), Query Frame = 0

Query: 1   MVAKLKSTAN----NNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 60
           MVA+ KSTA      ++NGR+LHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA
Sbjct: 1   MVAREKSTAKKLTATSNNGRELHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 60

Query: 61  RAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPS 120
           RAYDTAARQFRGAKAKTNFPLPE LI VSPH+I    + SPS SSTVESSSSSSQEK+PS
Sbjct: 61  RAYDTAARQFRGAKAKTNFPLPEELIEVSPHKI----NHSPSASSTVESSSSSSQEKSPS 120

Query: 121 PEIRR-------VARTLPLIQPQFSHH-------------GGGRPLLFMDGFVRPEFVA- 180
            EI R         RT P IQPQF                   RP+LFMD F RPEFVA 
Sbjct: 121 SEIFRGYGGALNAGRTFPFIQPQFQPQVMVGCNASASAAAANARPVLFMDAFGRPEFVAH 180

Query: 181 ---LRFDRATVDLTARFTGEIQR--------VDCRPAKETLNLDLNLAPPVD 197
              LRFD ATVDLTARF G I +        VDCRPAKE LNLDLNLAPPVD
Sbjct: 181 GYPLRFDPATVDLTARFAGGIHQSDSDSSSVVDCRPAKEILNLDLNLAPPVD 228

BLAST of Sed0005487.1 vs. ExPASy Swiss-Prot
Match: Q9LW49 (Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ERF4 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.6e-31
Identity = 97/224 (43.30%), Postives = 122/224 (54.46%), Query Frame = 0

Query: 10  NNNSNG-RDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQFRG 69
           N  +NG +++H+RGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEAA+AYDTAAR+FRG
Sbjct: 16  NVKTNGVKEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRG 75

Query: 70  AKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAP--SPEIRRVARTL 129
            KAKTNFPLP             S + S S SST+ESSS  +   AP  +P    + R L
Sbjct: 76  PKAKTNFPLP-------------SENQSTSHSSTMESSSGETGIHAPPHAPLELDLTRRL 135

Query: 130 PLI---------------------QPQFSHHGGGRPLLFMDGFVRPEFV----------- 189
             +                     QP  +    G+P+L  D   RP  V           
Sbjct: 136 GSVAADGGDNCRRSGEVGYPIFHQQPTVAVLPNGQPVLLFDSLWRPGVVNRPQPYHVMPM 195

Query: 190 ALRFD--RATVDLTARFTGEIQRVDCRPAKETLNLDLNLAPPVD 197
           A+ F+   A VD T   +  +   +    K  ++LDLNLAPP +
Sbjct: 196 AMGFNGVNAGVDPTVSDSSSVVEENQYDGKRGIDLDLNLAPPTE 226

BLAST of Sed0005487.1 vs. ExPASy Swiss-Prot
Match: Q40477 (Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 2.6e-29
Identity = 91/215 (42.33%), Postives = 115/215 (53.49%), Query Frame = 0

Query: 16  RDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQFRGAKAKTNF 75
           +++H+RGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEAA+AYDTAAR+FRG KAKTNF
Sbjct: 23  KEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNF 82

Query: 76  PLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAP--SPEIRRVARTLPLI---- 135
           P P             + + SPS SSTVESSS  +   AP  +P    + R L  +    
Sbjct: 83  PSP-------------TENQSPSHSSTVESSSGENGVHAPPHAPLELDLTRRLGSVAADG 142

Query: 136 -----------------QPQFSHHGGGRPLLFMDGFVRPEFV---------ALRFD--RA 195
                            QP  +    G+P+L  D   R   V          + F+   A
Sbjct: 143 GDNCRRSGEVGYPIFHQQPTVAVLPNGQPVLLFDSLWRAGVVNRPQPYHVTPMGFNGVNA 202

Query: 196 TVDLTARFTGEIQRVDCRPAKETLNLDLNLAPPVD 197
            V  T   +      +    K  ++LDLNLAPP++
Sbjct: 203 GVGPTVSDSSSAVEENQYDGKRGIDLDLNLAPPME 224

BLAST of Sed0005487.1 vs. ExPASy Swiss-Prot
Match: Q9FE67 (Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana OX=3702 GN=ERF9 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.8e-29
Identity = 85/180 (47.22%), Postives = 106/180 (58.89%), Query Frame = 0

Query: 16  RDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQFRGAKAKTNF 75
           +++HFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAR+FRG+KAKTNF
Sbjct: 29  KEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAREFRGSKAKTNF 88

Query: 76  PLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIRRVARTLPLIQPQFSH 135
           PLP     V+        + S    +TV ++   ++++ P    R            F  
Sbjct: 89  PLPGESTTVNDG----GENDSYVNRTTVTTAREMTRQRFPFACHRERKVVGGYASAGFFF 148

Query: 136 HGGGRPLLFMDGFVRPEFVALRFDRATVDLTARFTGEIQRVDCRPAKETLNLDLNLAPPV 195
                  L  +         +RFD   ++L+    G  + V   P +E LNLDLNLAPPV
Sbjct: 149 DPSRAASLRAE---LSRVCPVRFDPVNIELS---IGIRETVKVEPRRE-LNLDLNLAPPV 197

BLAST of Sed0005487.1 vs. ExPASy Swiss-Prot
Match: O80340 (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-28
Identity = 76/148 (51.35%), Postives = 96/148 (64.86%), Query Frame = 0

Query: 7   STANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQF 66
           +T   ++N +++ +RGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAR F
Sbjct: 12  TTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDF 71

Query: 67  RGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIRRVARTL 126
           RGAKAKTNFP     + +S  ++    + SPS SST++ +S  +    PS     V    
Sbjct: 72  RGAKAKTNFPT---FLELSDQKVPTGFARSPSQSSTLDCASPPTL-VVPSATAGNVP--- 131

Query: 127 PLIQPQFSHHGGG--------RPLLFMD 147
           P ++      GGG        RP+ F+D
Sbjct: 132 PQLELSLGGGGGGSCYQIPMSRPVYFLD 152

BLAST of Sed0005487.1 vs. ExPASy Swiss-Prot
Match: Q9MAI5 (Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ERF8 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.1e-25
Identity = 80/202 (39.60%), Postives = 108/202 (53.47%), Query Frame = 0

Query: 2   VAKLKSTANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDT 61
           VA    T + ++  +++ +RGVRKRPWGRYAAEIRDP KKTRVWLGTFDTA++AARAYD 
Sbjct: 13  VAPTNPTHHESNAAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDA 72

Query: 62  AARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIR- 121
           AAR FRG KAKTNF               +    SP+ SSTV  S ++++   P P +  
Sbjct: 73  AARDFRGVKAKTNF--------------GVIVGSSPTQSSTVVDSPTAARFITP-PHLEL 132

Query: 122 ------RVARTLPLIQPQFSHHGGGRPLLFMDGFVRPEFVALRFDRATVDLTARFTGEIQ 181
                    R +PL+ P + ++    P +   G V+ E        +       F G   
Sbjct: 133 SLGGGGACRRKIPLVHPVYYYNMATYPKMTTCG-VQSE--------SETSSVVDFEGGAG 185

Query: 182 RVDCRPAKETLNLDLNLAPPVD 197
           ++        L+LDLNLAPP +
Sbjct: 193 KI-----SPPLDLDLNLAPPAE 185

BLAST of Sed0005487.1 vs. ExPASy TrEMBL
Match: A0A5A7SPZ4 (Ethylene-responsive transcription factor 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003160 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.7e-71
Identity = 158/217 (72.81%), Postives = 171/217 (78.80%), Query Frame = 0

Query: 1   MVAKLKSTANN-----NSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEA 60
           MVAK KS+ NN     N+NG+DLHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEA
Sbjct: 1   MVAKEKSSPNNLPSLSNNNGKDLHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEA 60

Query: 61  ARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAP 120
           ARAYDTAARQFRGAKAKTNFP P+ LI VSPH+I    + SPSPSSTV+SSSSSS EK P
Sbjct: 61  ARAYDTAARQFRGAKAKTNFPPPDELIDVSPHKI----NQSPSPSSTVDSSSSSSHEKTP 120

Query: 121 SPEIRR---VARTLPLIQPQFSHHGGG--RPLLFMDGFVRPEFVA----LRFDRATVDLT 180
           SPEI R   V RT P IQPQF H GGG  RP+LF+D F +PEFVA    +RFD ATVDLT
Sbjct: 121 SPEISRSYGVGRTFPFIQPQFLHGGGGSVRPVLFVDVFGKPEFVAHGYPIRFDPATVDLT 180

Query: 181 ARFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
           +RFTG IQ        VD RPAKE LNLDLNLAPPVD
Sbjct: 181 SRFTGGIQSDSNSSSLVDFRPAKEILNLDLNLAPPVD 213

BLAST of Sed0005487.1 vs. ExPASy TrEMBL
Match: A0A1S3C1T3 (LOW QUALITY PROTEIN: ethylene-responsive transcription factor 4 OS=Cucumis melo OX=3656 GN=LOC103495486 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 3.1e-70
Identity = 156/217 (71.89%), Postives = 170/217 (78.34%), Query Frame = 0

Query: 1   MVAKLKSTAN-----NNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEA 60
           MVAK KS+       +N+NG+DLHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEEA
Sbjct: 1   MVAKEKSSPQQPPSLSNNNGKDLHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEA 60

Query: 61  ARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAP 120
           ARAYDTAARQFRGAKAKTNFP P+ LI VSPH+I    + SPSPSSTV+SSSSSS EK P
Sbjct: 61  ARAYDTAARQFRGAKAKTNFPPPDELIDVSPHKI----NQSPSPSSTVDSSSSSSHEKTP 120

Query: 121 SPEIRR---VARTLPLIQPQFSHHGGG--RPLLFMDGFVRPEFVA----LRFDRATVDLT 180
           SPEI R   V RT P IQPQF H GGG  RP+LF+D F +PEFVA    +RFD ATVDLT
Sbjct: 121 SPEISRSYGVGRTFPFIQPQFLHGGGGSVRPVLFVDVFGKPEFVAHGYPIRFDPATVDLT 180

Query: 181 ARFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
           +RFTG IQ        VD RPAKE LNLDLNLAPPVD
Sbjct: 181 SRFTGGIQSDSNSSSLVDFRPAKEILNLDLNLAPPVD 213

BLAST of Sed0005487.1 vs. ExPASy TrEMBL
Match: A0A6J1JNH6 (ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=LOC111486294 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 5.0e-68
Identity = 156/221 (70.59%), Postives = 166/221 (75.11%), Query Frame = 0

Query: 1   MVAKLKSTA-------NNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAE 60
           MV+K KSTA       N N+N R+LHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAE
Sbjct: 1   MVSKEKSTAKKLPPLCNTNNNARELHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAE 60

Query: 61  EAARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEK 120
           EAA AYD AARQFRGAKAKTNFPLP+ LI VSP +I    S SPSPSSTVESSSSSSQEK
Sbjct: 61  EAAYAYDNAARQFRGAKAKTNFPLPDELIDVSPRKI----SQSPSPSSTVESSSSSSQEK 120

Query: 121 APSPEIRR---VARTLPLIQPQFSHHGGG----RPLLFMDGFVRPEFVA----LRFDRAT 180
            PSPEI R   VART P IQPQF H  GG    RP+LFMD FVRPEFVA    +R+D   
Sbjct: 121 TPSPEIVRSYGVARTFPFIQPQFLHQVGGGGSARPVLFMDAFVRPEFVAQGYPIRYD--- 180

Query: 181 VDLTARFTGEIQR-------VDCRPAKETLNLDLNLAPPVD 197
              +ARF GEIQ        VDCRPAKE LNLDLNLAPPVD
Sbjct: 181 ---SARFAGEIQTKSDSLSVVDCRPAKEILNLDLNLAPPVD 211

BLAST of Sed0005487.1 vs. ExPASy TrEMBL
Match: V5QP37 (Ethylene-responsive transcription factor OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.6e-68
Identity = 157/232 (67.67%), Postives = 166/232 (71.55%), Query Frame = 0

Query: 1   MVAKLKSTAN----NNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 60
           MVA+ KSTA      ++NGR+LHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA
Sbjct: 1   MVAREKSTAKKLTATSNNGRELHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAA 60

Query: 61  RAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPS 120
           RAYDTAARQFRGAKAKTNFPLPE LI VSPH+I    + SPS SSTVESSSSSSQEK+PS
Sbjct: 61  RAYDTAARQFRGAKAKTNFPLPEELIEVSPHKI----NHSPSASSTVESSSSSSQEKSPS 120

Query: 121 PEIRR-------VARTLPLIQPQFSHH-------------GGGRPLLFMDGFVRPEFVA- 180
            EI R         RT P IQPQF                   RP+LFMD F RPEFVA 
Sbjct: 121 SEIFRGYGGALNAGRTFPFIQPQFQPQVMVGCNASASAAAANARPVLFMDAFGRPEFVAH 180

Query: 181 ---LRFDRATVDLTARFTGEIQR--------VDCRPAKETLNLDLNLAPPVD 197
              LRFD ATVDLTARF G I +        VDCRPAKE LNLDLNLAPPVD
Sbjct: 181 GYPLRFDPATVDLTARFAGGIHQSDSDSSSVVDCRPAKEILNLDLNLAPPVD 228

BLAST of Sed0005487.1 vs. ExPASy TrEMBL
Match: A0A6J1GM68 (ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN=LOC111455632 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.3e-67
Identity = 155/234 (66.24%), Postives = 166/234 (70.94%), Query Frame = 0

Query: 1   MVAKLKSTAN------NNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEE 60
           MVAK KS A       NN+  ++LHFRGVRKRPWGRYAAEIRDPGKK+RVWLGTFDTAEE
Sbjct: 1   MVAKEKSAAKSLPSLCNNTYAKELHFRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEE 60

Query: 61  AARAYDTAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKA 120
           AARAYD+AARQFRGAKAKTNFPL E LI VSP +I    + SPSPSSTVESSSSSSQEK 
Sbjct: 61  AARAYDSAARQFRGAKAKTNFPLEEELIDVSPQKI----NQSPSPSSTVESSSSSSQEKT 120

Query: 121 PSPEIRR-------VARTLPLIQPQFSHH--------------GGGRPLLFMDGFVRPEF 180
           PSPEI R       V RT P +QPQF H               GG RP+LFMD FVRPEF
Sbjct: 121 PSPEIARNYGATVGVGRTSPFLQPQFLHQVGCNVSAAAAAGSGGGVRPILFMDAFVRPEF 180

Query: 181 VA----LRFDRATVDLTARFTG-------EIQRVDCRPAKETLNLDLNLAPPVD 197
           VA    +RFD ATVDLTARF+G           VDCRP KE LNLDLNLAPPVD
Sbjct: 181 VAHGYPIRFDPATVDLTARFSGGSHSDSDSSSVVDCRPVKEILNLDLNLAPPVD 230

BLAST of Sed0005487.1 vs. TAIR 10
Match: AT5G44210.1 (erf domain protein 9 )

HSP 1 Score: 129.0 bits (323), Expect = 4.1e-30
Identity = 85/180 (47.22%), Postives = 106/180 (58.89%), Query Frame = 0

Query: 16  RDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQFRGAKAKTNF 75
           +++HFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAR+FRG+KAKTNF
Sbjct: 29  KEVHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAREFRGSKAKTNF 88

Query: 76  PLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIRRVARTLPLIQPQFSH 135
           PLP     V+        + S    +TV ++   ++++ P    R            F  
Sbjct: 89  PLPGESTTVNDG----GENDSYVNRTTVTTAREMTRQRFPFACHRERKVVGGYASAGFFF 148

Query: 136 HGGGRPLLFMDGFVRPEFVALRFDRATVDLTARFTGEIQRVDCRPAKETLNLDLNLAPPV 195
                  L  +         +RFD   ++L+    G  + V   P +E LNLDLNLAPPV
Sbjct: 149 DPSRAASLRAE---LSRVCPVRFDPVNIELS---IGIRETVKVEPRRE-LNLDLNLAPPV 197

BLAST of Sed0005487.1 vs. TAIR 10
Match: AT3G15210.1 (ethylene responsive element binding factor 4 )

HSP 1 Score: 127.1 bits (318), Expect = 1.6e-29
Identity = 76/148 (51.35%), Postives = 96/148 (64.86%), Query Frame = 0

Query: 7   STANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQF 66
           +T   ++N +++ +RGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAR F
Sbjct: 12  TTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARDF 71

Query: 67  RGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIRRVARTL 126
           RGAKAKTNFP     + +S  ++    + SPS SST++ +S  +    PS     V    
Sbjct: 72  RGAKAKTNFPT---FLELSDQKVPTGFARSPSQSSTLDCASPPTL-VVPSATAGNVP--- 131

Query: 127 PLIQPQFSHHGGG--------RPLLFMD 147
           P ++      GGG        RP+ F+D
Sbjct: 132 PQLELSLGGGGGGSCYQIPMSRPVYFLD 152

BLAST of Sed0005487.1 vs. TAIR 10
Match: AT1G53170.1 (ethylene response factor 8 )

HSP 1 Score: 115.9 bits (289), Expect = 3.6e-26
Identity = 80/202 (39.60%), Postives = 108/202 (53.47%), Query Frame = 0

Query: 2   VAKLKSTANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDT 61
           VA    T + ++  +++ +RGVRKRPWGRYAAEIRDP KKTRVWLGTFDTA++AARAYD 
Sbjct: 13  VAPTNPTHHESNAAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTAQQAARAYDA 72

Query: 62  AARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEKAPSPEIR- 121
           AAR FRG KAKTNF               +    SP+ SSTV  S ++++   P P +  
Sbjct: 73  AARDFRGVKAKTNF--------------GVIVGSSPTQSSTVVDSPTAARFITP-PHLEL 132

Query: 122 ------RVARTLPLIQPQFSHHGGGRPLLFMDGFVRPEFVALRFDRATVDLTARFTGEIQ 181
                    R +PL+ P + ++    P +   G V+ E        +       F G   
Sbjct: 133 SLGGGGACRRKIPLVHPVYYYNMATYPKMTTCG-VQSE--------SETSSVVDFEGGAG 185

Query: 182 RVDCRPAKETLNLDLNLAPPVD 197
           ++        L+LDLNLAPP +
Sbjct: 193 KI-----SPPLDLDLNLAPPAE 185

BLAST of Sed0005487.1 vs. TAIR 10
Match: AT1G28370.1 (ERF domain protein 11 )

HSP 1 Score: 114.8 bits (286), Expect = 8.0e-26
Identity = 70/127 (55.12%), Postives = 85/127 (66.93%), Query Frame = 0

Query: 1   MVAKLKSTANNNSNGRDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYD 60
           M   +K+ A   + G  + +RGVRKRPWGRYAAEIRDP KK+RVWLGTFDT EEAARAYD
Sbjct: 1   MAPTVKTAAVKTNEGNGVRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYD 60

Query: 61  TAARQFRGAKAKTNFPLPEHLIHVSPHQISLSPSPSPSPSSTVESS-------SSSSQEK 120
             A +FRGAKAKTNFP      +++ H +SL  + S S SSTVESS       S S   +
Sbjct: 61  KRAIEFRGAKAKTNFP----CYNINAHCLSL--TQSLSQSSTVESSFPNLNLGSDSVSSR 120

BLAST of Sed0005487.1 vs. TAIR 10
Match: AT1G28360.1 (ERF domain protein 12 )

HSP 1 Score: 105.5 bits (262), Expect = 4.9e-23
Identity = 64/126 (50.79%), Postives = 76/126 (60.32%), Query Frame = 0

Query: 16  RDLHFRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAARQFRGAKAKTNF 75
           R++H+RGVRKRPWGRYAAEIRDP KKTRVWLGTFDT EEAA AYD AAR  RG KAKTNF
Sbjct: 8   REVHYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDTPEEAALAYDGAARFLRGIKAKTNF 67

Query: 76  PLPEHLIHVSPHQISLSPSPSPSPSSTVESSSSSSQEK-----APSPEIRRVARTLPLIQ 135
           P        SP  + L+  PS   ++T  +++   Q +     AP P           + 
Sbjct: 68  P--------SPLSLDLNHLPSAPSAATAAANNQPHQHQQLWFAAPPP-----------VP 114

Query: 136 PQFSHH 137
           P   HH
Sbjct: 128 PSSDHH 114

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0031547.13.4e-7172.81ethylene-responsive transcription factor 4 [Cucumis melo var. makuwa] >TYK07000.... [more]
XP_038886999.11.7e-7072.69ethylene-responsive transcription factor 4-like [Benincasa hispida][more]
XP_008455284.26.5e-7071.89PREDICTED: LOW QUALITY PROTEIN: ethylene-responsive transcription factor 4 [Cucu... [more]
XP_022989129.11.0e-6770.59ethylene-responsive transcription factor 4-like [Cucurbita maxima][more]
AHB18381.11.8e-6767.67ethylene-responsive transcription factor [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LW491.6e-3143.30Ethylene-responsive transcription factor 4 OS=Nicotiana sylvestris OX=4096 GN=ER... [more]
Q404772.6e-2942.33Ethylene-responsive transcription factor 4 OS=Nicotiana tabacum OX=4097 GN=ERF4 ... [more]
Q9FE675.8e-2947.22Ethylene-responsive transcription factor 9 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
O803402.2e-2851.35Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Q9MAI55.1e-2539.60Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Match NameE-valueIdentityDescription
A0A5A7SPZ41.7e-7172.81Ethylene-responsive transcription factor 4 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3C1T33.1e-7071.89LOW QUALITY PROTEIN: ethylene-responsive transcription factor 4 OS=Cucumis melo ... [more]
A0A6J1JNH65.0e-6870.59ethylene-responsive transcription factor 4-like OS=Cucurbita maxima OX=3661 GN=L... [more]
V5QP378.6e-6867.67Ethylene-responsive transcription factor OS=Momordica charantia OX=3673 PE=2 SV=... [more]
A0A6J1GM683.3e-6766.24ethylene-responsive transcription factor 4-like OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G44210.14.1e-3047.22erf domain protein 9 [more]
AT3G15210.11.6e-2951.35ethylene responsive element binding factor 4 [more]
AT1G53170.13.6e-2639.60ethylene response factor 8 [more]
AT1G28370.18.0e-2655.12ERF domain protein 11 [more]
AT1G28360.14.9e-2350.79ERF domain protein 12 [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 42..58
score: 76.36
coord: 20..31
score: 60.91
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 19..82
e-value: 1.6E-36
score: 137.3
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 19..68
e-value: 7.4E-15
score: 54.9
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 19..76
score: 23.735794
IPR001471AP2/ERF domainCDDcd00018AP2coord: 19..76
e-value: 1.02104E-31
score: 107.743
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 19..77
e-value: 1.7E-28
score: 100.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..115
NoneNo IPR availablePANTHERPTHR31677AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 2..104
NoneNo IPR availablePANTHERPTHR31677:SF46AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 2..104
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 19..77

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Sed0005487Sed0005487gene


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0005487.1-three_prime_utrSed0005487.1-three_prime_utr-LG04:43782098..43782715three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0005487.1-exonSed0005487.1-exon-LG04:43782098..43783387exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0005487.1-cdsSed0005487.1-cds-LG04:43782716..43783309CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Sed0005487.1-five_prime_utrSed0005487.1-five_prime_utr-LG04:43783310..43783387five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Sed0005487.1Sed0005487.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity