Sed0004458.1 (mRNA) Chayote v1

Overview


Name	Sed0004458.1
Type	mRNA
Organism	Sechium edule (Chayote v1)
Description	butyrate--CoA ligase AAE11, peroxisomal-like
Location	LG03: 5689952 .. 5692691 (+)
Sequence length	1020
RNA-Seq Expression	Sed0004458.1
Synteny	Sed0004458.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCATCTTTCAAAATGTGATGCAAACTATGCTCCTCTTACACCCATCACCTTCCTTAGGAGAGCTTCGAAGTGTTATGCAGATCGAATCTCGATTATCTATGAAAGAACTCGTTTCACATGGCGCCAAACAGATGAGCGTTGTCGTCGTCTCGCCTCCTCCCTTCATCGTCTCAATGTCACGAAGAATGACGTTGTAAGACCCTCTTTCTCTATTTCTTGCGTTTTTTATTTTAGATTATTTGAAAAATTGAATGCTAGGTTATTATATAGTTATAAAGTGAAAACTCAAATAAAATCTTCCTCATAAGTTTATACAACAATTATAGTTTCTCTTAACTTTTATTATTGTGTAATTTAATCATGTAATACCAAATGATTGACTTATGATCATTTATATATTGAGCAGCAATAACTAATTATTTAAAATAACATGTCTATGTTGATATTAAAACTACGAAACATTAATATATTTGATGAAATGTCTTTGAACTAGGTCGCATTACAAATATTAATTACATGGGTTTAGCTTAATATTCTTATCTATTTACTTTTTCCACTGTTTCTAAATAGGTTTATGTTCTTTATTTGTTAATAAAACTAAAACATCTAATTTTCCTTAATTACGTACAGATTGCTTCCCATTATTTTAAAAACCATAGCAATCCCAAACAAAGCCTAAATGATTAGAAATTTAAAGAAAATTATTATTTAAAAAAATAACATTTATAATTTAAATTTCCTCTAAATCTCTAAAAAATATATACATGATTTTTACTTTTAACATTTTAAATCAGCATACAAATATTGAGACACTAAATTCTCAAGAGAAGAATTAAAACTAAAGATGTTCACAAGGTTCCATCCTCTTCAAAGTTTACTACTTTTTATAGAAATTGAGGAAGGGATTTGACTTGATAAATTATTTTCCATTCAAATGTTTTTCTAATCTATATAAATATTTTATTAATAATATTTTCAAATAATTAATTTTTAATAAATAAATATAAATTGATTTTCAATTAGATACTTAATTATTAAAGAAAAGTACCCATTCAAACAAAAATTTCTACCTACAAAATTTTATCTAAAGATTAAATTGGTTTAAAAAAATGAAAAATGAAAACACTTCTTTTTTCTTTTATAAATATATATATATATATATATATATATATATATATATATATATATAAATTTTGTAGGTAAGCTTTGAGTATTTTTCTCCTATTTACTCCCACATTCTCTACCTAAACAAGATTTTACCATCTATTAAGACTGATCACTGCATTAAATGAACCACAATTATAAATCATACATCTAATCAAATTTTAGCAAACAAACATTATTGACCTTACATAATTTGATCTGAGTAACGTATTATATTATTTACTAAAGAAGTATATGAATCAAATTCGTAATAAATTAATGACTCACATGTTGCGTTGAACCAATTAAACGGATACATTCACATTTTAGTTAATGATCACAAATCTCATGCCTTGATATTCACGTTTGATTCTCAAAGGTGTCAGTATTAGCATCCAACATTCCAGCATTATACGACATGCATTTTGCAGTACCGATGGCAGGTGCCATTCTAAACCCAATCGACACCCACTTTGACGTCAATAACATCACCACAATCCTTCACCACTCCAAGGCCAAGGTCTTCTTTGTCGACTACCAATACATTCAACAAGCCAAAGATGCCATTCGTGTACTACTTGCAGAGTCCTCCGTAGTCCCGCTCGTCGTCGTCATCGACGACATCGACTCGCCGAGTGGAGCCCGACTTGGAAACATGGAATACGAGGAACTCATCCGCGACGGAGACGTCGGGTTCGCAGCGGAGGATGTCGAGAACGAATGGGACTCGATATCCTTGAACTACACCTCCGGCACGACCGCGGCGCCTAAGGGCGTGGTGTACAGCCACCGCAGCGCGTTCCTCAGCACACTCGGCATGATGATCGGTTGGGAAATGGAAAACGCGCCGGTGTTTCTGTGGACCCACCCGATGTTCCACTGCAACGGCTGGACCTTCCCGTGGGTTGAAATTTATTCAATTTTTTTTAGCATTTTTGTAAATTTAACCTCTACATGACAACAATTTAGAATATATTTAAAATTAAATTTGGTAGGTTGTTGTTTGTTAGAGTTAATTAATTTTAAAATTTGAACGTTAACCAACCATAGTTCATTTCGATAGACAATAAAAAAAATCATGGATTTGAATCTTTTGATGGTTAAACTAAAGAAGTATAATTTTATAGAAAGAAATCAAATTTGAAAAAATTAATCTTAATTACATAGAAATAATTGACAAGTTAATAACTCTTATCAAAATTCACTATCAGGAATCAACATTTTGACATTAACTGACGTGGATGTCAAAAACTTGGACACAATGGAGAGCATGCCGCACGACGGGAAAACCATAGGCAAGATCGGTCATACGTGGGAGCAGGATCTTAAAAGGCTACTTCAATAATCCAGCAGCAACAGAGACAGCCTTGAGAAATGGGTGGTTTTACACCGGCGACGTCGGCGTCATTCACCCCGATGGCTATTTAGAAGTCAAAGATCGATCCAAAGATGTCATCAACTCTGCTGGTGAGAACATGAGTAGTGCGGAGCTCGAGGAAGTACTCTACAACCAGCGGTGGTTGGTATGCGACTCCCATGTGGGGAAGAGAGCCCTTGTGCCTTTGTCGTCGTCAATGGCGAAGCGTGCGTGA

mRNA sequence

ATGGAGCATCTTTCAAAATGTGATGCAAACTATGCTCCTCTTACACCCATCACCTTCCTTAGGAGAGCTTCGAAGTGTTATGCAGATCGAATCTCGATTATCTATGAAAGAACTCGTTTCACATGGCGCCAAACAGATGAGCGTTGTCGTCGTCTCGCCTCCTCCCTTCATCGTCTCAATGTCACGAAGAATGACGTTGTGTCAGTATTAGCATCCAACATTCCAGCATTATACGACATGCATTTTGCAGTACCGATGGCAGGTGCCATTCTAAACCCAATCGACACCCACTTTGACGTCAATAACATCACCACAATCCTTCACCACTCCAAGGCCAAGGTCTTCTTTGTCGACTACCAATACATTCAACAAGCCAAAGATGCCATTCGTGTACTACTTGCAGAGTCCTCCGTAGTCCCGCTCGTCGTCGTCATCGACGACATCGACTCGCCGAGTGGAGCCCGACTTGGAAACATGGAATACGAGGAACTCATCCGCGACGGAGACGTCGGGTTCGCAGCGGAGGATGTCGAGAACGAATGGGACTCGATATCCTTGAACTACACCTCCGGCACGACCGCGGCGCCTAAGGGCGTGGTGTACAGCCACCGCAGCGCGTTCCTCAGCACACTCGGCATGATGATCGGTTGGGAAATGGAAAACGCGCCGGTGTTTCTGTGGACCCACCCGATGTTCCACTGCAACGGCTGGACCTTCCCGTGGGCAAGATCGGTCATACGTGGGAGCAGGATCTTAAAAGGCTACTTCAATAATCCAGCAGCAACAGAGACAGCCTTGAGAAATGGGTGGTTTTACACCGGCGACGTCGGCGTCATTCACCCCGATGGCTATTTAGAAGTCAAAGATCGATCCAAAGATGTCATCAACTCTGCTGGTGAGAACATGAGTAGTGCGGAGCTCGAGGAAGTACTCTACAACCAGCGGTGGTTGGTATGCGACTCCCATGTGGGGAAGAGAGCCCTTGTGCCTTTGTCGTCGTCAATGGCGAAGCGTGCGTGA

Coding sequence (CDS)

ATGGAGCATCTTTCAAAATGTGATGCAAACTATGCTCCTCTTACACCCATCACCTTCCTTAGGAGAGCTTCGAAGTGTTATGCAGATCGAATCTCGATTATCTATGAAAGAACTCGTTTCACATGGCGCCAAACAGATGAGCGTTGTCGTCGTCTCGCCTCCTCCCTTCATCGTCTCAATGTCACGAAGAATGACGTTGTGTCAGTATTAGCATCCAACATTCCAGCATTATACGACATGCATTTTGCAGTACCGATGGCAGGTGCCATTCTAAACCCAATCGACACCCACTTTGACGTCAATAACATCACCACAATCCTTCACCACTCCAAGGCCAAGGTCTTCTTTGTCGACTACCAATACATTCAACAAGCCAAAGATGCCATTCGTGTACTACTTGCAGAGTCCTCCGTAGTCCCGCTCGTCGTCGTCATCGACGACATCGACTCGCCGAGTGGAGCCCGACTTGGAAACATGGAATACGAGGAACTCATCCGCGACGGAGACGTCGGGTTCGCAGCGGAGGATGTCGAGAACGAATGGGACTCGATATCCTTGAACTACACCTCCGGCACGACCGCGGCGCCTAAGGGCGTGGTGTACAGCCACCGCAGCGCGTTCCTCAGCACACTCGGCATGATGATCGGTTGGGAAATGGAAAACGCGCCGGTGTTTCTGTGGACCCACCCGATGTTCCACTGCAACGGCTGGACCTTCCCGTGGGCAAGATCGGTCATACGTGGGAGCAGGATCTTAAAAGGCTACTTCAATAATCCAGCAGCAACAGAGACAGCCTTGAGAAATGGGTGGTTTTACACCGGCGACGTCGGCGTCATTCACCCCGATGGCTATTTAGAAGTCAAAGATCGATCCAAAGATGTCATCAACTCTGCTGGTGAGAACATGAGTAGTGCGGAGCTCGAGGAAGTACTCTACAACCAGCGGTGGTTGGTATGCGACTCCCATGTGGGGAAGAGAGCCCTTGTGCCTTTGTCGTCGTCAATGGCGAAGCGTGCGTGA

Protein sequence

MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLNVTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQYIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFPWARSVIRGSRILKGYFNNPAATETALRNGWFYTGDVGVIHPDGYLEVKDRSKDVINSAGENMSSAELEEVLYNQRWLVCDSHVGKRALVPLSSSMAKRA

Homology

[Close]

BLAST of Sed0004458.1 vs. NCBI nr
Match: XP_011655134.1 (butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetical protein Csa_023114 [Cucumis sativus])

HSP 1 Score: 424.9 bits (1091), Expect = 6.7e-115
Identity = 230/458 (50.22%), Postives = 269/458 (58.73%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           ME L KCDANY+ LTPITFL+RAS  YADR SIIYE TRFTW+QT ERC RLASSL RL 
Sbjct: 1   MEMLLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLT 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V+KNDVVSVLA N+PALY+MHFAVPMAGAILN I+T  D+ NI  IL HS+AK+FFVDYQ
Sbjct: 61  VSKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           YIQ+AKDA+R+L+AESS +PLVVVIDDID+P+GARLGN+EYE+LI DGD  F   +V++E
Sbjct: 121 YIQEAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR AFLSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. NCBI nr
Match: XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 420.6 bits (1080), Expect = 1.3e-113
Identity = 230/458 (50.22%), Postives = 263/458 (57.42%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M  LSKCDANYA LTPITFL+RAS  YADR SIIYE TRFTWRQT ERC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DV+NI  IL HS+AK+ FVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           +IQ+AKDA+R L+A S  +P VVVIDDIDSP+GAR G +EYE+L+ DGDVGF   +V++E
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR A+LSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 1.3e-113
Identity = 230/458 (50.22%), Postives = 263/458 (57.42%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M  LSKCDANYA LTPITFL+RAS  YADR SIIYE TRFTWRQT +RC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DVNNI  IL HS+AK+ FVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           +IQ+AKDA+R L+A S  +P VVVIDDIDSP+GAR G +EYE+L+ DGDVGF   +V++E
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR A+LSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. NCBI nr
Match: XP_008463169.1 (PREDICTED: butyrate--CoA ligase AAE11, peroxisomal-like [Cucumis melo] >KAA0046225.1 butyrate--CoA ligase AAE11, peroxisomal-like [Cucumis melo var. makuwa])

HSP 1 Score: 419.9 bits (1078), Expect = 2.2e-113
Identity = 228/458 (49.78%), Postives = 264/458 (57.64%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           ME L KCDANY  LTPITFL+RAS  YADR SIIYE TRFTW QT ERC RLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V+KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DV NI  IL HS+AK+FFVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           YIQ+AKDA+R+L+AES  +PLVVVIDDID+P+GARLGN+EYE+LI DGD  F   +V++E
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR AFLSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-112
Identity = 229/458 (50.00%), Postives = 262/458 (57.21%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M  LSKCDANYA LTPITFL+RAS  YADR SIIYE TRFTWRQT +RC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DVNNI  IL HS+AK+ FVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           +IQ+AKDA+R L+A S  +P VVVIDDIDSP+GAR G +EYE+L+ DGDVGF   +V++E
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR A+LSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.7e-85
Identity = 185/468 (39.53%), Postives = 235/468 (50.21%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M+ L KC ANY PLTP+TFL RA K YA+R SIIY   RFTW QT +RC RLASSL  LN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           + KNDVVSVLA N+PA Y+MHFAVPMAGA+LN I+T  D  NI  IL HS+AK+ FVDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YIQQAKDAIRVLLAESSV---------VPLVVVIDDIDSPSG-ARLGNMEYEELIRDGDV 180
           Y+++A+ A+ +L++ + +         +P V++IDD+ SP+   +   +EYE+L+  G+ 
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 GFAAED-VENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTH 240
            +A E+ V++EWD I LNYTSGTT+ PKGVVYSHR AFLSTL  ++GWEM   PV+LW+ 
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFPWA----------------------------------------------- 300
           PMFH NGWT  W                                                
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 ------------------------------------------------------------ 312
                                                                       
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 9.2e-83
Identity = 177/463 (38.23%), Postives = 234/463 (50.54%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M++L  C+AN  PLTPITFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           +T+NDVVS+LA N+PA+Y+MHF+VPM GA+LNPI+T  D   I  IL H++ K+ FVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKDAIRVL-LAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAE---- 180
           +    ++ +R++   +S   P +++I++IDS +      ++YE LIR G+   ++     
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 DVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCN 240
            V NE D ISLNYTSGTTA PKGVV SH+ A+LS L  +IGWEM   PV+LWT PMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFPWA----------------------------------------------------- 300
           GWT  W+                                                     
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.7e-82
Identity = 180/463 (38.88%), Postives = 233/463 (50.32%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M+ L+ C+AN  PLTP+TFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           ++KNDVVSV+A N PALY+MHFAVPMAGA+LNPI+T  D  +I  IL H+K K+ F+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKDAIRVLLAESSVVPL-VVVIDDIDSPSGARLGNMEYEELIRDGD----VGFAAE 180
           +   A++++ +L +E S + L V+ I + D P  A    ++YE LI+ G+    +     
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 DVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCN 240
            +++E D ISLNYTSGTTA PKGVV SHR A+L TL  +IGWEM   PV+LWT PMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFPWARS--------------------------------------------------- 300
           GWTF W  +                                                   
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.1e-80
Identity = 177/463 (38.23%), Postives = 232/463 (50.11%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M++L+ C+AN  PLTPITFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           + KNDVVSV+A N PA+Y+MHFAVPMAGA+LNPI+T  D  +I  IL H+K K+ F+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKDAIRVLLAESSVVPL-VVVIDDIDSPSGARLGNMEYEELIRDGD----VGFAAE 180
           +   A++ +++L +E S + L V+ I +ID P        +YE LI+ G+    +     
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 DVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCN 240
            +++E D ISLNYTSGTTA PKGVV SHR A+LSTL  +IGWEM   PV+LWT PMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFPWARS--------------------------------------------------- 300
           GWTF W  +                                                   
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy Swiss-Prot
Match: Q9C9G2 (Probable acyl-activating enzyme 22 OS=Arabidopsis thaliana OX=3702 GN=AEE22 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.8e-77
Identity = 168/434 (38.71%), Postives = 226/434 (52.07%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M+++  C+AN  PLTPITFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDNMELCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           + KNDVVSV+A N PA+Y+MHFAVPMAGA+LNPI+T  D  +ITTIL H++ K+ F+   
Sbjct: 61  IAKNDVVSVVAPNTPAIYEMHFAVPMAGAVLNPINTRLDATSITTILRHAQPKILFIHRN 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVV-IDDIDSPSGARLGNMEYEELIRDGDVGFAAED--- 180
           +   A++ + +L  +   + L+V+ ID+ +S        ++YE LI+ G+      +   
Sbjct: 121 FEPLAREILHLLSCDDLQLNLLVIFIDEYNSAKRVSSEELDYESLIQMGEPTSPLVENMF 180

Query: 181 -VENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLW-------- 240
            ++NE D ISLNYTSGTTA PKGVV SHR A+L++LG++IGWEM   PV+LW        
Sbjct: 181 RIQNEQDPISLNYTSGTTADPKGVVISHRGAYLTSLGVIIGWEMSTCPVYLWIFAYVSLQ 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 WMDVYMGNSSARGHQCVYEPRNPLDMSHRSGPVHLMTGGSPLPAALVKKVQRLGFQVLHV 300

Query: 301 ------THPMFHCNGWTFPWAR-------------------------------------- 313
                 T P   C  W   W R                                      
Sbjct: 301 YGLTEATGPALFCE-WQDEWNRLTENQQMELKARQGLGILSVAEVDVKYNETQESVPHDG 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.7e-114
Identity = 228/455 (50.11%), Postives = 267/455 (58.68%), Query Frame = 0


Query: 4   LSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLNVTK 63
           L KCDANY+ LTPITFL+RAS  YADR SIIYE TRFTW+QT ERC RLASSL RL V+K
Sbjct: 2   LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61

Query: 64  NDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQYIQ 123
           NDVVSVLA N+PALY+MHFAVPMAGAILN I+T  D+ NI  IL HS+AK+FFVDYQYIQ
Sbjct: 62  NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121

Query: 124 QAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENEWDS 183
           +AKDA+R+L+AESS +PLVVVIDDID+P+GARLGN+EYE+LI DGD  F   +V++EWDS
Sbjct: 122 EAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWDS 181

Query: 184 ISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFPW-- 243
           I+LNYTSGTT+APKGVVYSHR AFLSTL +++GWEM NAPV+LWT PMFHCNGWTF W  
Sbjct: 182 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 241

Query: 244 ------------------------------------------------------------ 303
                                                                       
Sbjct: 242 AARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLTG 301

Query: 304 ------------------------------------------------------------ 313
                                                                       
Sbjct: 302 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGISI 361

[Close]

BLAST of Sed0004458.1 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 6.1e-114
Identity = 230/458 (50.22%), Postives = 263/458 (57.42%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M  LSKCDANYA LTPITFL+RAS  YADR SIIYE TRFTWRQT +RC RLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DVNNI  IL HS+AK+ FVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           +IQ+AKDA+R L+A S  +P VVVIDDIDSP+GAR G +EYE+L+ DGDVGF   +V++E
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR A+LSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 1.0e-113
Identity = 228/458 (49.78%), Postives = 264/458 (57.64%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           ME L KCDANY  LTPITFL+RAS  YADR SIIYE TRFTW QT ERC RLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V+KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DV NI  IL HS+AK+FFVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           YIQ+AKDA+R+L+AES  +PLVVVIDDID+P+GARLGN+EYE+LI DGD  F   +V++E
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR AFLSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 1.0e-113
Identity = 228/458 (49.78%), Postives = 264/458 (57.64%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           ME L KCDANY  LTPITFL+RAS  YADR SIIYE TRFTW QT ERC RLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V+KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DV NI  IL HS+AK+FFVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           YIQ+AKDA+R+L+AES  +PLVVVIDDID+P+GARLGN+EYE+LI DGD  F   +V++E
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR AFLSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 5.7e-112
Identity = 227/458 (49.56%), Postives = 261/458 (56.99%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M  LSKCDAN+A LTPITFL+RAS  YADR SIIYE T FTWRQT ERC  LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           V KN VVSVLA N+PALY+MHFAVPMAGAILN I+T  DV+NI  IL HS+AK+ FVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAEDVENE 180
           +IQ+AKDA+R L+A S  +P VVVIDDIDSP+GAR G +EYE+L+ DGDVGF   +V++E
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNGWTFP 240
           WDSI+LNYTSGTT+APKGVVYSHR A+LSTL +++GWEM NAPV+LWT PMFHCNGWTF 
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 W----------------------------------------------------------- 300
           W                                                           
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

[Close]

BLAST of Sed0004458.1 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 308.5 bits (789), Expect = 6.5e-84
Identity = 177/463 (38.23%), Postives = 234/463 (50.54%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M++L  C+AN  PLTPITFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           +T+NDVVS+LA N+PA+Y+MHF+VPM GA+LNPI+T  D   I  IL H++ K+ FVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKDAIRVL-LAESSVVPLVVVIDDIDSPSGARLGNMEYEELIRDGDVGFAAE---- 180
           +    ++ +R++   +S   P +++I++IDS +      ++YE LIR G+   ++     
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 DVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCN 240
            V NE D ISLNYTSGTTA PKGVV SH+ A+LS L  +IGWEM   PV+LWT PMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFPWA----------------------------------------------------- 300
           GWT  W+                                                     
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

[Close]

BLAST of Sed0004458.1 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-83
Identity = 180/463 (38.88%), Postives = 233/463 (50.32%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M+ L+ C+AN  PLTP+TFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           ++KNDVVSV+A N PALY+MHFAVPMAGA+LNPI+T  D  +I  IL H+K K+ F+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKDAIRVLLAESSVVPL-VVVIDDIDSPSGARLGNMEYEELIRDGD----VGFAAE 180
           +   A++++ +L +E S + L V+ I + D P  A    ++YE LI+ G+    +     
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 DVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCN 240
            +++E D ISLNYTSGTTA PKGVV SHR A+L TL  +IGWEM   PV+LWT PMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFPWARS--------------------------------------------------- 300
           GWTF W  +                                                   
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

[Close]

BLAST of Sed0004458.1 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 301.6 bits (771), Expect = 8.0e-82
Identity = 177/463 (38.23%), Postives = 232/463 (50.11%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M++L+ C+AN  PLTPITFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           + KNDVVSV+A N PA+Y+MHFAVPMAGA+LNPI+T  D  +I  IL H+K K+ F+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKDAIRVLLAESSVVPL-VVVIDDIDSPSGARLGNMEYEELIRDGD----VGFAAE 180
           +   A++ +++L +E S + L V+ I +ID P        +YE LI+ G+    +     
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 DVENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCN 240
            +++E D ISLNYTSGTTA PKGVV SHR A+LSTL  +IGWEM   PV+LWT PMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFPWARS--------------------------------------------------- 300
           GWTF W  +                                                   
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

[Close]

BLAST of Sed0004458.1 vs. TAIR 10
Match: AT1G68270.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.1e-78
Identity = 168/434 (38.71%), Postives = 226/434 (52.07%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           M+++  C+AN  PLTPITFL+RAS+CY +R SIIY +TRFTW QT +RC RLA+SL  LN
Sbjct: 1   MDNMELCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           + KNDVVSV+A N PA+Y+MHFAVPMAGA+LNPI+T  D  +ITTIL H++ K+ F+   
Sbjct: 61  IAKNDVVSVVAPNTPAIYEMHFAVPMAGAVLNPINTRLDATSITTILRHAQPKILFIHRN 120

Query: 121 YIQQAKDAIRVLLAESSVVPLVVV-IDDIDSPSGARLGNMEYEELIRDGDVGFAAED--- 180
           +   A++ + +L  +   + L+V+ ID+ +S        ++YE LI+ G+      +   
Sbjct: 121 FEPLAREILHLLSCDDLQLNLLVIFIDEYNSAKRVSSEELDYESLIQMGEPTSPLVENMF 180

Query: 181 -VENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLW-------- 240
            ++NE D ISLNYTSGTTA PKGVV SHR A+L++LG++IGWEM   PV+LW        
Sbjct: 181 RIQNEQDPISLNYTSGTTADPKGVVISHRGAYLTSLGVIIGWEMSTCPVYLWIFAYVSLQ 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 WMDVYMGNSSARGHQCVYEPRNPLDMSHRSGPVHLMTGGSPLPAALVKKVQRLGFQVLHV 300

Query: 301 ------THPMFHCNGWTFPWAR-------------------------------------- 313
                 T P   C  W   W R                                      
Sbjct: 301 YGLTEATGPALFCE-WQDEWNRLTENQQMELKARQGLGILSVAEVDVKYNETQESVPHDG 360

[Close]

BLAST of Sed0004458.1 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 263.8 bits (673), Expect = 1.8e-70
Identity = 154/462 (33.33%), Postives = 220/462 (47.62%), Query Frame = 0


Query: 1   MEHLSKCDANYAPLTPITFLRRASKCYADRISIIYERTRFTWRQTDERCRRLASSLHRLN 60
           ME   K  ANY PLTPI+FL R++  YADR+SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  VTKNDVVSVLASNIPALYDMHFAVPMAGAILNPIDTHFDVNNITTILHHSKAKVFFVDYQ 120
           ++  DVVSVLA N+PA+ ++HF VPMAGA+L  ++   D + +  +L HS  KV F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKDAIRVLLAESSVVPLVVVIDDIDSPSGARLGN----MEYEELIRDGDVGFAAED 180
           ++Q A+ A  +L  +   VP++V+I +  + S +R       MEYE+++  G   F    
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 VENEWDSISLNYTSGTTAAPKGVVYSHRSAFLSTLGMMIGWEMENAPVFLWTHPMFHCNG 240
             +E D+IS+NYTSGTT++PKGVVYSHR A+L++L  ++  EM ++P +LWT+PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFPW------------------------------------------------------- 300
           W   W                                                       
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 ------------------------------------------------------------ 313
                                                                       
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

The following BLAST results are available for this feature:

BLAST of Sed0004458.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011655134.1	6.7e-115	50.22	butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetic...	[more]
XP_023513711.1	1.3e-113	50.22	butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]
XP_022923630.1	1.3e-113	50.22	butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata]	[more]
XP_008463169.1	2.2e-113	49.78	PREDICTED: butyrate--CoA ligase AAE11, peroxisomal-like [Cucumis melo] >KAA00462...	[more]
KAG6602004.1	1.8e-112	50.00	Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Sed0004458.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I3PB36	5.7e-85	39.53	Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 S...	[more]
Q9C8D4	9.2e-83	38.23	Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11...	[more]
Q9SS01	2.7e-82	38.88	Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 ...	[more]
Q9SS00	1.1e-80	38.23	Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9C9G2	5.8e-77	38.71	Probable acyl-activating enzyme 22 OS=Arabidopsis thaliana OX=3702 GN=AEE22 PE=3...	[more]

BLAST of Sed0004458.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KT40	2.7e-114	50.11	Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 G...	[more]
A0A6J1ECD9	6.1e-114	50.22	butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A5A7TXW0	1.0e-113	49.78	Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A1S3CIM1	1.0e-113	49.78	butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A6J1JUR4	5.7e-112	49.56	butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of Sed0004458.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66120.1	6.5e-84	38.23	AMP-dependent synthetase and ligase family protein	[more]
AT1G65880.1	1.9e-83	38.88	benzoyloxyglucosinolate 1	[more]
AT1G65890.1	8.0e-82	38.23	acyl activating enzyme 12	[more]
AT1G68270.1	4.1e-78	38.71	AMP-dependent synthetase and ligase family protein	[more]
AT1G20560.1	1.8e-70	33.33	acyl activating enzyme 1	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.30.38.10	Luciferase; Domain 3	coord: 244..300 e-value: 2.9E-14 score: 54.9
None	No IPR available	PANTHER	PTHR43859:SF2	BUTYRATE--COA LIGASE AAE11, PEROXISOMAL	coord: 1..242 coord: 245..315
None	No IPR available	PANTHER	PTHR43859	ACYL-ACTIVATING ENZYME	coord: 1..242 coord: 245..315
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 245..313
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 10..241
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 3..243 e-value: 1.6E-45 score: 157.3
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 21..239 e-value: 7.7E-41 score: 140.1 coord: 245..297 e-value: 5.2E-16 score: 58.3

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Sed0004458	Sed0004458	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0004458.1-exon	Sed0004458.1-exon-LG03:5689952..5690149	exon
Sed0004458.1-exon	Sed0004458.1-exon-LG03:5691479..5692003	exon
Sed0004458.1-exon	Sed0004458.1-exon-LG03:5692395..5692691	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Sed0004458.1-cds	Sed0004458.1-cds-LG03:5689952..5690149	CDS
Sed0004458.1-cds	Sed0004458.1-cds-LG03:5691479..5692003	CDS
Sed0004458.1-cds	Sed0004458.1-cds-LG03:5692395..5692691	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Sed0004458.1	Sed0004458.1-protein	polypeptide