PI0027987.1 (mRNA) Melon (PI 482460) v1

BLAST of PI0027987.1 vs. ExPASy Swiss-Prot
Match: O80624 (Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 GN=MAKR4 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.2e-40
Identity = 140/379 (36.94%), Postives = 197/379 (51.98%), Query Frame = 0

Query: 14  EEDYIDMELTSSPSSKSFCYSIGSPPPTPPQAARDFEFQFQMTSFSG-ETEATTSPADDL 73
           EEDYIDME+TS  +      S   P           EF+FQM+     E + TTSPAD+L
Sbjct: 12  EEDYIDMEVTSFTNLVRKTLSNNYPR----------EFEFQMSHLCPLEIDKTTSPADEL 71

Query: 74  FYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFISIKAPPPESCNVSP 133
           FYKGKLLPLHLPPRLQMVQK+L      +        +     P  S    P ESC VSP
Sbjct: 72  FYKGKLLPLHLPPRLQMVQKILEDYTFDDEFYSTPLATGTVTTPVTS--NTPFESCTVSP 131

Query: 134 SESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTKSWSKKVKQLKQS--------SQAYFK 193
           +ESC+ S ELNP+++ L +   + +D       KSW+ K++ +KQS        S+AY +
Sbjct: 132 AESCQVSKELNPEDYFLEYSDSLEED---DEKKKSWTTKLRLMKQSSLGTKIKASRAYLR 191

Query: 194 SLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQIPNQTLEK 253
           S FGK+ CS  ++SS  +       E+       ++ S+  PFG+I            + 
Sbjct: 192 SFFGKTSCS--DESSCASSAARVADED-----SVLRYSRVKPFGQI------------KT 251

Query: 254 EKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTSSSGSSSLS 313
           E+ ++   G ++  +HRRSFS +++R ++ SS     NNK S+S                
Sbjct: 252 ERPKKQSNGSVS-GSHRRSFSVSMRRQAAKSS-----NNKSSNSL--------------- 311

Query: 314 SSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNETESQPQTCS 373
                   GF  LQ  KRS SS+SEIENSI+GAI HCKQSQ+     ++  T ++   CS
Sbjct: 312 --------GFRPLQFLKRSTSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCS 327

Query: 374 LSVSKVLPCGDQKSSQLCR 384
           LS S++    DQ+ +Q+ R
Sbjct: 372 LSASRIAARDDQEWAQMFR 327

BLAST of PI0027987.1 vs. ExPASy Swiss-Prot
Match: Q9ZUS8 (Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 GN=MAKR3 PE=2 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 9.2e-31
Identity = 144/378 (38.10%), Postives = 187/378 (49.47%), Query Frame = 0

Query: 14  EEDYIDME--LTSSPSSKSFCYSIGSPPPT--PPQAARDFEFQFQMTSFSGETEATTSPA 73
           ++ YIDME  L+SS SS +   S  S P T  PPQ +R+FEFQ   +S     E+TTSPA
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQ-SREFEFQ-MCSSAVASGESTTSPA 77

Query: 74  DDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFISIKAPPPESCN 133
           D+LFYKG+LLPLHLPPRL+MVQKLL + +S   +          E P     +P   +  
Sbjct: 78  DELFYKGQLLPLHLPPRLKMVQKLLLASSSSTAAT---------ETPI----SPRAAADV 137

Query: 134 VSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTT---KSWSKKVK-----QLKQSSQA 193
           +SP      SCE+  DE         +K F+         SWSKK+K     Q  ++S+A
Sbjct: 138 LSPRRF--SSCEIGQDENCFFEISTELKRFIESNENHLGNSWSKKIKHSSITQKLKASRA 197

Query: 194 YFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQIPNQT 253
           Y K+LF K  CS +++ +                                      P   
Sbjct: 198 YIKALFSKQACSDSSEIN--------------------------------------PRFK 257

Query: 254 LEKEKAEEFYGGILNINN----HRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTSS 313
           +E  K        +N  N    HRRSFSG IQR S              + CS+SSS+SS
Sbjct: 258 IEPSKVSRKKNPFVNSENPLLIHRRSFSGVIQRHS-------------QAKCSTSSSSSS 317

Query: 314 SGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNETE 373
           S +SSLSSSFSF SNG  DLQ+  RS +++   +NSIEGAI HCKQS       + N TE
Sbjct: 318 S-ASSLSSSFSFGSNGSLDLQTLMRSSNAS---DNSIEGAIEHCKQS---FTTRKSNVTE 317

Query: 374 SQPQTCSLSVSKVLPCGD 376
           S  + CS S + V  CGD
Sbjct: 378 S--ELCS-SRTSVSTCGD 317

BLAST of PI0027987.1 vs. ExPASy TrEMBL
Match: A0A0A0L234 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G293210 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.7e-195
Identity = 370/388 (95.36%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MATANVPFATAPAEEDYIDMELTSSPSSKSFCYSIGS--PPPTPPQAARDFEFQFQMTSF 60
           MATAN PFA+APAEEDYIDMELTSSPSSKSFCYSIGS  PPP PPQAARDFEFQFQMTSF
Sbjct: 1   MATANAPFASAPAEEDYIDMELTSSPSSKSFCYSIGSPPPPPPPPQAARDFEFQFQMTSF 60

Query: 61  SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI 120
           SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI
Sbjct: 61  SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI 120

Query: 121 SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPT--TTKSWSKKVKQLK 180
           SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPT  TTKSWSKKVKQLK
Sbjct: 121 SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTATTKSWSKKVKQLK 180

Query: 181 QSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQ 240
           QSSQAYFKSLFGKSVCSSNN+SSVFNEETENIFENR +SGKFIKVSKKTPFGRIDYNKWQ
Sbjct: 181 QSSQAYFKSLFGKSVCSSNNRSSVFNEETENIFENRRNSGKFIKVSKKTPFGRIDYNKWQ 240

Query: 241 IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTS 300
           IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNK SSSC    STS
Sbjct: 241 IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKTSSSC----STS 300

Query: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNET 360
           SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNE+
Sbjct: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNES 360

Query: 361 ESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           ESQ QTCSLSVSKVLPCGDQK+SQLCRI
Sbjct: 361 ESQLQTCSLSVSKVLPCGDQKTSQLCRI 384

BLAST of PI0027987.1 vs. ExPASy TrEMBL
Match: A0A5A7TV49 (Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G001530 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.7e-166
Identity = 318/332 (95.78%), Postives = 323/332 (97.29%), Query Frame = 0

Query: 55  MTSFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 114
           MTSFSGETEA TSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE
Sbjct: 1   MTSFSGETEAATSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 60

Query: 115 IPFISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP--TTTKSWSKKV 174
           IPFIS+KAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP  TTTKSWSKKV
Sbjct: 61  IPFISMKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTTTKSWSKKV 120

Query: 175 KQLKQSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDY 234
           KQLKQSSQAYFKSLFGKSVCSSNN+SSVFNEE+ENIF+NRGSSGKFIKVSKK PFGRIDY
Sbjct: 121 KQLKQSSQAYFKSLFGKSVCSSNNRSSVFNEESENIFDNRGSSGKFIKVSKKNPFGRIDY 180

Query: 235 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSS 294
           NKWQIPNQTLEKEKAE+FYGGILNINNHRRSFSGAIQRTSSSSSSS NNNNKISSSC   
Sbjct: 181 NKWQIPNQTLEKEKAEDFYGGILNINNHRRSFSGAIQRTSSSSSSSINNNNKISSSC--- 240

Query: 295 SSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 354
            STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR
Sbjct: 241 -STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 300

Query: 355 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI
Sbjct: 301 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 328

BLAST of PI0027987.1 vs. ExPASy TrEMBL
Match: A0A1S3BHC7 (LOW QUALITY PROTEIN: probable membrane-associated kinase regulator 4 OS=Cucumis melo OX=3656 GN=LOC103489854 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.6e-164
Identity = 316/332 (95.18%), Postives = 322/332 (96.99%), Query Frame = 0

Query: 55  MTSFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 114
           MTSFSGETEA TSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE
Sbjct: 1   MTSFSGETEAATSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 60

Query: 115 IPFISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP--TTTKSWSKKV 174
           IPFIS+KAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP  TTTKSWSKKV
Sbjct: 61  IPFISMKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTTTKSWSKKV 120

Query: 175 KQLKQSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDY 234
           KQLKQSSQAYFKSLFGKSVCSSNN+SSVFNEE+ENIF+NRGSSGKFIKV +K PFGRIDY
Sbjct: 121 KQLKQSSQAYFKSLFGKSVCSSNNRSSVFNEESENIFDNRGSSGKFIKV-QKNPFGRIDY 180

Query: 235 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSS 294
           NKWQIPNQTLEKEKAE+FYGGILNINNHRRSFSGAIQRTSSSSSSS NNNNKISSSC   
Sbjct: 181 NKWQIPNQTLEKEKAEDFYGGILNINNHRRSFSGAIQRTSSSSSSSINNNNKISSSC--- 240

Query: 295 SSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 354
            STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR
Sbjct: 241 -STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 300

Query: 355 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI
Sbjct: 301 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 327

BLAST of PI0027987.1 vs. ExPASy TrEMBL
Match: A0A6J1GG61 (probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN=LOC111453872 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.4e-130
Identity = 280/385 (72.73%), Postives = 303/385 (78.70%), Query Frame = 0

Query: 1   MATANVPFATAPAEEDYIDMELTSSPSSKSFCYSIGSPPPTPPQAARDFEFQFQMTSFSG 60
           MAT   PF TAPAEEDYIDMELTSSPSS SF YSI SPPP     ARDFEFQ QMTSFSG
Sbjct: 1   MATTEAPFTTAPAEEDYIDMELTSSPSSNSFSYSIRSPPP-----ARDFEFQIQMTSFSG 60

Query: 61  ETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFISI 120
           ETE TTSPADDLFYKGKLLPLHLPPRLQMVQ LLHSPNS NPS K +SF ENF+IPFI+I
Sbjct: 61  ETEQTTSPADDLFYKGKLLPLHLPPRLQMVQNLLHSPNSHNPSTKNKSFPENFQIPFIAI 120

Query: 121 KAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTKSWSKKVKQLKQSSQ 180
           KAPP ESCN SP+ES R SCELNPDE+LL FRGE IKDFV     KSWS KVKQLKQSSQ
Sbjct: 121 KAPPHESCNFSPTESSRASCELNPDEYLLRFRGE-IKDFVGNPPRKSWSNKVKQLKQSSQ 180

Query: 181 AYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQIPNQ 240
           AYFK+LFGKS CS+    S+FNEETE  F+NR SSGKFI    KTPFGRIDYNKW+I   
Sbjct: 181 AYFKALFGKSPCST---KSLFNEETECGFKNRVSSGKFI---NKTPFGRIDYNKWKIQTT 240

Query: 241 TLEKEKAEEFYGGILNI-NNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTSSSG 300
            +EKEK EEF G  LN+ NNHRRSFSGAIQ  SSS SS +  ++   SSCS S S+SSSG
Sbjct: 241 FIEKEKTEEFSGSSLNMNNNHRRSFSGAIQTASSSCSSCSLCSS--CSSCSCSCSSSSSG 300

Query: 301 SSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNETESQ 360
           SSSLSSSF           SFKRS S+NSEIE SIEGAIAHCKQSQK++ NSRK E ES 
Sbjct: 301 SSSLSSSF-----------SFKRSNSANSEIERSIEGAIAHCKQSQKVLDNSRKREAESS 360

Query: 361 PQTCSLSVSKVLPCGDQKSSQLCRI 385
           P+TC +SVSKVLPCG+QKS +LCRI
Sbjct: 361 PETCCVSVSKVLPCGNQKSYELCRI 360

BLAST of PI0027987.1 vs. ExPASy TrEMBL
Match: A0A6J1IMI8 (probable membrane-associated kinase regulator 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478391 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.2e-129
Identity = 280/386 (72.54%), Postives = 303/386 (78.50%), Query Frame = 0

Query: 1   MATANVPFATAPAEEDYIDMELTSSPSSKSFCYSIGSPPPTPPQAARDFEFQFQMTSFSG 60
           MAT   PF  APAEEDYIDMELTSSPSS SF YSI SPPP P   ARDFEFQ QMTSFSG
Sbjct: 1   MATTEAPFTAAPAEEDYIDMELTSSPSSNSFSYSIRSPPP-PLPPARDFEFQIQMTSFSG 60

Query: 61  ETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFISI 120
           ETE TTSPADDLFYKGKLLPLHLPPRLQMVQ LLHSPNSQNPSRK +SF EN +IPFI+I
Sbjct: 61  ETEPTTSPADDLFYKGKLLPLHLPPRLQMVQNLLHSPNSQNPSRKNKSFPENLQIPFIAI 120

Query: 121 KAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTKSWSKKVKQLKQSSQ 180
           KAPP ESCN SP+ESCR SCELNPDE+LL FRGE IKDFV     KSWS KVKQLKQSSQ
Sbjct: 121 KAPPHESCNFSPTESCRASCELNPDEYLLRFRGE-IKDFVGNPPRKSWSNKVKQLKQSSQ 180

Query: 181 AYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQIPNQ 240
           AYFK+LFGKS CSS    S+FNEETE +F+NR SS KFI    KTPFGRIDYNKW+I + 
Sbjct: 181 AYFKALFGKSPCSS---KSLFNEETECVFKNRVSSWKFI---NKTPFGRIDYNKWKIQST 240

Query: 241 TLEKEKAEEFYGGILNI-NNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTSSSG 300
            +EKEK EEF    LN+ NNHRRSFSGAIQ  SSS SS ++      SSCS S S+SSSG
Sbjct: 241 FIEKEKTEEFSSSSLNMNNNHRRSFSGAIQTASSSCSSCSS-----CSSCSCSCSSSSSG 300

Query: 301 SSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNETESQ 360
           SSSLSSSF           SFKRS S+NSEIE SIEGAIAHCKQSQK++ NSRK E ES 
Sbjct: 301 SSSLSSSF-----------SFKRSNSANSEIERSIEGAIAHCKQSQKVLDNSRKREAESS 360

Query: 361 PQTCSLSVSK-VLPCGDQKSSQLCRI 385
           P+TC +SVSK VLPCG+QKS +L RI
Sbjct: 361 PETCGVSVSKVVLPCGNQKSYELRRI 362

BLAST of PI0027987.1 vs. NCBI nr
Match: KGN54211.1 (hypothetical protein Csa_018049 [Cucumis sativus])

HSP 1 Score: 690.3 bits (1780), Expect = 9.7e-195
Identity = 370/388 (95.36%), Postives = 375/388 (96.65%), Query Frame = 0

Query: 1   MATANVPFATAPAEEDYIDMELTSSPSSKSFCYSIGS--PPPTPPQAARDFEFQFQMTSF 60
           MATAN PFA+APAEEDYIDMELTSSPSSKSFCYSIGS  PPP PPQAARDFEFQFQMTSF
Sbjct: 1   MATANAPFASAPAEEDYIDMELTSSPSSKSFCYSIGSPPPPPPPPQAARDFEFQFQMTSF 60

Query: 61  SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI 120
           SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI
Sbjct: 61  SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI 120

Query: 121 SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPT--TTKSWSKKVKQLK 180
           SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPT  TTKSWSKKVKQLK
Sbjct: 121 SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTATTKSWSKKVKQLK 180

Query: 181 QSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQ 240
           QSSQAYFKSLFGKSVCSSNN+SSVFNEETENIFENR +SGKFIKVSKKTPFGRIDYNKWQ
Sbjct: 181 QSSQAYFKSLFGKSVCSSNNRSSVFNEETENIFENRRNSGKFIKVSKKTPFGRIDYNKWQ 240

Query: 241 IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTS 300
           IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNK SSSC    STS
Sbjct: 241 IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKTSSSC----STS 300

Query: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNET 360
           SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNE+
Sbjct: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNES 360

Query: 361 ESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           ESQ QTCSLSVSKVLPCGDQK+SQLCRI
Sbjct: 361 ESQLQTCSLSVSKVLPCGDQKTSQLCRI 384

BLAST of PI0027987.1 vs. NCBI nr
Match: XP_038883185.1 (probable membrane-associated kinase regulator 4 [Benincasa hispida])

HSP 1 Score: 613.6 bits (1581), Expect = 1.2e-171
Identity = 337/395 (85.32%), Postives = 358/395 (90.63%), Query Frame = 0

Query: 1   MATANVPFATAPAEEDYIDMELTSSPSSKSFCYSIGSP-PPTPP--QAARDFEFQFQMTS 60
           MATA  PFA  PAEEDYIDMELTSSPSS SFCYSI SP PP+PP   AARDFEFQFQMTS
Sbjct: 1   MATAEAPFAALPAEEDYIDMELTSSPSSNSFCYSIRSPTPPSPPAASAARDFEFQFQMTS 60

Query: 61  FSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPF 120
           FS ETEA TSPADDLFYKGKLLPLHLPPRLQMVQKLLHSP SQ+PSRK ESFSENFE+PF
Sbjct: 61  FSDETEAITSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPKSQDPSRKTESFSENFEMPF 120

Query: 121 ISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFV---NPTTTKSWSKKVKQ 180
           I+IKAPP ESCNVSPSESCR SCELNPD++LLGFRGEIIKDFV   +P  TKSWSKKVKQ
Sbjct: 121 ITIKAPPHESCNVSPSESCRASCELNPDDYLLGFRGEIIKDFVVGRDPPRTKSWSKKVKQ 180

Query: 181 LKQSSQAYFKSLFGKSVC--SSNNKS---SVFNEETENIFENRGSSGKFIKVSKKTPFGR 240
           LKQSSQAYFK+LFGKS C  S+NNKS   SVFNEE+++IFE+  SSGKFIKVSKK PFGR
Sbjct: 181 LKQSSQAYFKALFGKSACSTSTNNKSSVNSVFNEESQSIFESERSSGKFIKVSKKAPFGR 240

Query: 241 IDYNKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSC 300
           IDYNKWQIPN  LEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSS+++ +NNNK SSSC
Sbjct: 241 IDYNKWQIPNNALEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSTNNNSNNNKSSSSC 300

Query: 301 SSSSSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIV 360
           SS SSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSE ENSIEGAIAHCKQSQK+I 
Sbjct: 301 SSCSSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSERENSIEGAIAHCKQSQKLID 360

Query: 361 NSRKNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           NS+KNE+E+QPQTCSLSVSKVLPCGDQKSSQLCRI
Sbjct: 361 NSKKNESETQPQTCSLSVSKVLPCGDQKSSQLCRI 395

BLAST of PI0027987.1 vs. NCBI nr
Match: XP_004142216.2 (probable membrane-associated kinase regulator 4 [Cucumis sativus])

HSP 1 Score: 606.3 bits (1562), Expect = 1.8e-169
Identity = 323/388 (83.25%), Postives = 328/388 (84.54%), Query Frame = 0

Query: 1   MATANVPFATAPAEEDYIDMELTSSPSSKSFCYSIGS--PPPTPPQAARDFEFQFQMTSF 60
           MATAN PFA+APAEEDYIDMELTSSPSSKSFCYSIGS  PPP PPQAARDFEFQFQMTSF
Sbjct: 1   MATANAPFASAPAEEDYIDMELTSSPSSKSFCYSIGSPPPPPPPPQAARDFEFQFQMTSF 60

Query: 61  SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI 120
           SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI
Sbjct: 61  SGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFI 120

Query: 121 SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPT--TTKSWSKKVKQLK 180
           SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPT  TTKSWSKKVKQLK
Sbjct: 121 SIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTATTKSWSKKVKQLK 180

Query: 181 QSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQ 240
           QSSQAYFKSLFGKSVCSSNN+SSVFNEETENIFENR +SGKFIKVSKKTPFGRIDYNKWQ
Sbjct: 181 QSSQAYFKSLFGKSVCSSNNRSSVFNEETENIFENRRNSGKFIKVSKKTPFGRIDYNKWQ 240

Query: 241 IPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTS 300
           IPNQTLEKEKAEEFYGGILNINNHRRSFSG                              
Sbjct: 241 IPNQTLEKEKAEEFYGGILNINNHRRSFSG------------------------------ 300

Query: 301 SSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNET 360
                                 SFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNE+
Sbjct: 301 ----------------------SFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNES 336

Query: 361 ESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           ESQ QTCSLSVSKVLPCGDQK+SQLCRI
Sbjct: 361 ESQLQTCSLSVSKVLPCGDQKTSQLCRI 336

BLAST of PI0027987.1 vs. NCBI nr
Match: KAA0047332.1 (putative membrane-associated kinase regulator 4 [Cucumis melo var. makuwa])

HSP 1 Score: 594.7 bits (1532), Expect = 5.5e-166
Identity = 318/332 (95.78%), Postives = 323/332 (97.29%), Query Frame = 0

Query: 55  MTSFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 114
           MTSFSGETEA TSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE
Sbjct: 1   MTSFSGETEAATSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 60

Query: 115 IPFISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP--TTTKSWSKKV 174
           IPFIS+KAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP  TTTKSWSKKV
Sbjct: 61  IPFISMKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTTTKSWSKKV 120

Query: 175 KQLKQSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDY 234
           KQLKQSSQAYFKSLFGKSVCSSNN+SSVFNEE+ENIF+NRGSSGKFIKVSKK PFGRIDY
Sbjct: 121 KQLKQSSQAYFKSLFGKSVCSSNNRSSVFNEESENIFDNRGSSGKFIKVSKKNPFGRIDY 180

Query: 235 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSS 294
           NKWQIPNQTLEKEKAE+FYGGILNINNHRRSFSGAIQRTSSSSSSS NNNNKISSSC   
Sbjct: 181 NKWQIPNQTLEKEKAEDFYGGILNINNHRRSFSGAIQRTSSSSSSSINNNNKISSSC--- 240

Query: 295 SSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 354
            STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR
Sbjct: 241 -STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 300

Query: 355 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI
Sbjct: 301 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 328

BLAST of PI0027987.1 vs. NCBI nr
Match: XP_008447396.2 (PREDICTED: LOW QUALITY PROTEIN: probable membrane-associated kinase regulator 4 [Cucumis melo])

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-163
Identity = 316/332 (95.18%), Postives = 322/332 (96.99%), Query Frame = 0

Query: 55  MTSFSGETEATTSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 114
           MTSFSGETEA TSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE
Sbjct: 1   MTSFSGETEAATSPADDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFE 60

Query: 115 IPFISIKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP--TTTKSWSKKV 174
           IPFIS+KAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNP  TTTKSWSKKV
Sbjct: 61  IPFISMKAPPPESCNVSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTTTKSWSKKV 120

Query: 175 KQLKQSSQAYFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDY 234
           KQLKQSSQAYFKSLFGKSVCSSNN+SSVFNEE+ENIF+NRGSSGKFIKV +K PFGRIDY
Sbjct: 121 KQLKQSSQAYFKSLFGKSVCSSNNRSSVFNEESENIFDNRGSSGKFIKV-QKNPFGRIDY 180

Query: 235 NKWQIPNQTLEKEKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSS 294
           NKWQIPNQTLEKEKAE+FYGGILNINNHRRSFSGAIQRTSSSSSSS NNNNKISSSC   
Sbjct: 181 NKWQIPNQTLEKEKAEDFYGGILNINNHRRSFSGAIQRTSSSSSSSINNNNKISSSC--- 240

Query: 295 SSTSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 354
            STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR
Sbjct: 241 -STSSSGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSR 300

Query: 355 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 385
           KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI
Sbjct: 301 KNETESQPQTCSLSVSKVLPCGDQKSSQLCRI 327

BLAST of PI0027987.1 vs. TAIR 10
Match: AT2G39370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37380.1); Has 184 Blast hits to 178 proteins in 53 species: Archae - 0; Bacteria - 58; Metazoa - 9; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 167.9 bits (424), Expect = 1.6e-41
Identity = 140/379 (36.94%), Postives = 197/379 (51.98%), Query Frame = 0

Query: 14  EEDYIDMELTSSPSSKSFCYSIGSPPPTPPQAARDFEFQFQMTSFSG-ETEATTSPADDL 73
           EEDYIDME+TS  +      S   P           EF+FQM+     E + TTSPAD+L
Sbjct: 12  EEDYIDMEVTSFTNLVRKTLSNNYPR----------EFEFQMSHLCPLEIDKTTSPADEL 71

Query: 74  FYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFISIKAPPPESCNVSP 133
           FYKGKLLPLHLPPRLQMVQK+L      +        +     P  S    P ESC VSP
Sbjct: 72  FYKGKLLPLHLPPRLQMVQKILEDYTFDDEFYSTPLATGTVTTPVTS--NTPFESCTVSP 131

Query: 134 SESCRPSCELNPDEFLLGFRGEIIKDFVNPTTTKSWSKKVKQLKQS--------SQAYFK 193
           +ESC+ S ELNP+++ L +   + +D       KSW+ K++ +KQS        S+AY +
Sbjct: 132 AESCQVSKELNPEDYFLEYSDSLEED---DEKKKSWTTKLRLMKQSSLGTKIKASRAYLR 191

Query: 194 SLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQIPNQTLEK 253
           S FGK+ CS  ++SS  +       E+       ++ S+  PFG+I            + 
Sbjct: 192 SFFGKTSCS--DESSCASSAARVADED-----SVLRYSRVKPFGQI------------KT 251

Query: 254 EKAEEFYGGILNINNHRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTSSSGSSSLS 313
           E+ ++   G ++  +HRRSFS +++R ++ SS     NNK S+S                
Sbjct: 252 ERPKKQSNGSVS-GSHRRSFSVSMRRQAAKSS-----NNKSSNSL--------------- 311

Query: 314 SSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNETESQPQTCS 373
                   GF  LQ  KRS SS+SEIENSI+GAI HCKQSQ+     ++  T ++   CS
Sbjct: 312 --------GFRPLQFLKRSTSSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCS 327

Query: 374 LSVSKVLPCGDQKSSQLCR 384
           LS S++    DQ+ +Q+ R
Sbjct: 372 LSASRIAARDDQEWAQMFR 327

BLAST of PI0027987.1 vs. TAIR 10
Match: AT2G37380.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39370.1); Has 1284 Blast hits to 422 proteins in 114 species: Archae - 0; Bacteria - 90; Metazoa - 125; Fungi - 151; Plants - 136; Viruses - 0; Other Eukaryotes - 782 (source: NCBI BLink). )

HSP 1 Score: 136.0 bits (341), Expect = 6.6e-32
Identity = 144/378 (38.10%), Postives = 187/378 (49.47%), Query Frame = 0

Query: 14  EEDYIDME--LTSSPSSKSFCYSIGSPPPT--PPQAARDFEFQFQMTSFSGETEATTSPA 73
           ++ YIDME  L+SS SS +   S  S P T  PPQ +R+FEFQ   +S     E+TTSPA
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQ-SREFEFQ-MCSSAVASGESTTSPA 77

Query: 74  DDLFYKGKLLPLHLPPRLQMVQKLLHSPNSQNPSRKAESFSENFEIPFISIKAPPPESCN 133
           D+LFYKG+LLPLHLPPRL+MVQKLL + +S   +          E P     +P   +  
Sbjct: 78  DELFYKGQLLPLHLPPRLKMVQKLLLASSSSTAAT---------ETPI----SPRAAADV 137

Query: 134 VSPSESCRPSCELNPDEFLLGFRGEIIKDFVNPTTT---KSWSKKVK-----QLKQSSQA 193
           +SP      SCE+  DE         +K F+         SWSKK+K     Q  ++S+A
Sbjct: 138 LSPRRF--SSCEIGQDENCFFEISTELKRFIESNENHLGNSWSKKIKHSSITQKLKASRA 197

Query: 194 YFKSLFGKSVCSSNNKSSVFNEETENIFENRGSSGKFIKVSKKTPFGRIDYNKWQIPNQT 253
           Y K+LF K  CS +++ +                                      P   
Sbjct: 198 YIKALFSKQACSDSSEIN--------------------------------------PRFK 257

Query: 254 LEKEKAEEFYGGILNINN----HRRSFSGAIQRTSSSSSSSTNNNNKISSSCSSSSSTSS 313
           +E  K        +N  N    HRRSFSG IQR S              + CS+SSS+SS
Sbjct: 258 IEPSKVSRKKNPFVNSENPLLIHRRSFSGVIQRHS-------------QAKCSTSSSSSS 317

Query: 314 SGSSSLSSSFSFSSNGFCDLQSFKRSISSNSEIENSIEGAIAHCKQSQKMIVNSRKNETE 373
           S +SSLSSSFSF SNG  DLQ+  RS +++   +NSIEGAI HCKQS       + N TE
Sbjct: 318 S-ASSLSSSFSFGSNGSLDLQTLMRSSNAS---DNSIEGAIEHCKQS---FTTRKSNVTE 317

Query: 374 SQPQTCSLSVSKVLPCGD 376
           S  + CS S + V  CGD
Sbjct: 378 S--ELCS-SRTSVSTCGD 317