PI0021801.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0021801.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Glutamyl-tRNA(Gln) amidotransferase subunit A
Location	chr01: 5369174 .. 5371125 (-)
Sequence length	1557
RNA-Seq Expression	PI0021801.1
Synteny	PI0021801.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGACTCTTCACCACTTAGTTCTGTAGCCTTCTCATTGCTTCTGATTCTCGTGGCCTTCTGTTTGACTCCACCACATTCTGTAACGGTTCGAGGATTGTCAATTAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCGAGCAAAACCAACTCACTTCGAGGGAACTTGTCAACTTCTACATTGGAGAAATTCGCAGGCTCAATCCGGTTGTCCATGGGGTCATAGAAATCAACCCAGATGCATTGCTGCAAGCTTACAAGGCTGACAGAGAACGAGAGGCTAATAAGCCTGGATCGCTTGGTGGGTTGCACGGAATTCCAATTCTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCATTCGCATTGCTTGGGTCTATCGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAGAGCTGGAGCTATCATACTAGGAAAGGCCAGCTTGACTGAATGGGCTGATTTTAGGTCCCTCACTGCTCCAGCTGGTTTGAGCGCTAGAGGTGGTCAAGGAAAGGTGTGTTACTTCGAATTGGCTCCAAAGACAATTTATCCACACATTCAATGTTTTTCATGTACAACTAAAAACCATTTAATTTCTACAATTCTTTTCAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACCGATGGTTCTATCCTCTGTCCGGCTAGTTTTAACTCAGTTGTTGGCATCAAACCAACGGTCGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGTGAGCAATCTTGTACCCATATCAGCAAAACATTCTACAAAGATTATAAGAACTAAACCTTTGTAACCTTCGTATGTGAAAACAGGCCAATTGGCAGGACAGTGACAGATGCTGTGGTAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAGAACAGCCTCTAAGTACATTCCATATGGTGGCTACAAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAGAGATTAGGAATTGTAAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAGCAATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGTAAGAAAACCAACATCCCTACCTATCCATATAATTCCCCCAAAATCTTTCTATCATATTCATTTAAACAAACAATTCAACGTGAGCACAACCAATCTACACAAAGAATCTAACTAACATTGTTGAAATTGAAGGCAAGGCGGTGCTATTTTGATAGACAATCTAGAGATAGCAAATATAGATATCATCTTAAACGTAACGGCAAGTGGAGAAGCAGCAGCATTGCTTGCTGAATTCAAACAATCGTTAAATGGGTACCTGAAAGAGCTTGTGGTTTCCCCTGTTCGAAGTTTAGCCGACATAATCGCCTTCAATAACGCAAATGCAGATCAGGTAACACAATATTCAATCCATTTTTCAACGCATAATGATCGTATTAAATCCTAATCAATTCAAATCATGGCTGTGCAGGAACTTCTCAACGTTTTCGGCCAGGAGATCTTTCTGGCAGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGGGAAGCTAGCAGAAGATGGGTTTGAGAAATTAGTGAAGGAGAAGCGGTTGGATGCGGTGGTGACGCCAGGTTCCGGCATAGCTACAGTGCTTGCAATTGGGGGTTTACCGGGGATCAATGTTCCGGCGGGATACGATGGCGGAGGAGTTCCGTTTGGGATTAACTTTGGAGGGTTGAAGGGATCAGAGGCGAAGCTGATTGAAGTTGCTTATGGGTTTGAGCAAGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAACCTTAA

mRNA sequence

ATGGCCGACTCTTCACCACTTAGTTCTGTAGCCTTCTCATTGCTTCTGATTCTCGTGGCCTTCTGTTTGACTCCACCACATTCTGTAACGGTTCGAGGATTGTCAATTAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCGAGCAAAACCAACTCACTTCGAGGGAACTTGTCAACTTCTACATTGGAGAAATTCGCAGGCTCAATCCGGTTGTCCATGGGGTCATAGAAATCAACCCAGATGCATTGCTGCAAGCTTACAAGGCTGACAGAGAACGAGAGGCTAATAAGCCTGGATCGCTTGGTGGGTTGCACGGAATTCCAATTCTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCATTCGCATTGCTTGGGTCTATCGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAGAGCTGGAGCTATCATACTAGGAAAGGCCAGCTTGACTGAATGGGCTGATTTTAGGTCCCTCACTGCTCCAGCTGGTTTGAGCGCTAGAGGTGGTCAAGGAAAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACCGATGGTTCTATCCTCTGTCCGGCTAGTTTTAACTCAGTTGTTGGCATCAAACCAACGGTCGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATTGGCAGGACAGTGACAGATGCTGTGGTAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAGAACAGCCTCTAAGTACATTCCATATGGTGGCTACAAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAGAGATTAGGAATTGTAAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAGCAATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGCAAGGCGGTGCTATTTTGATAGACAATCTAGAGATAGCAAATATAGATATCATCTTAAACGTAACGGCAAGTGGAGAAGCAGCAGCATTGCTTGCTGAATTCAAACAATCGTTAAATGGGTACCTGAAAGAGCTTGTGGTTTCCCCTGTTCGAAGTTTAGCCGACATAATCGCCTTCAATAACGCAAATGCAGATCAGGAACTTCTCAACGTTTTCGGCCAGGAGATCTTTCTGGCAGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGGGAAGCTAGCAGAAGATGGGTTTGAGAAATTAGTGAAGGAGAAGCGGTTGGATGCGGTGGTGACGCCAGGTTCCGGCATAGCTACAGTGCTTGCAATTGGGGGTTTACCGGGGATCAATGTTCCGGCGGGATACGATGGCGGAGGAGTTCCGTTTGGGATTAACTTTGGAGGGTTGAAGGGATCAGAGGCGAAGCTGATTGAAGTTGCTTATGGGTTTGAGCAAGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAACCTTAA

Coding sequence (CDS)

ATGGCCGACTCTTCACCACTTAGTTCTGTAGCCTTCTCATTGCTTCTGATTCTCGTGGCCTTCTGTTTGACTCCACCACATTCTGTAACGGTTCGAGGATTGTCAATTAGAGAAGCCACAGTGCACGACCTCCAGCTAGCTTTCGAGCAAAACCAACTCACTTCGAGGGAACTTGTCAACTTCTACATTGGAGAAATTCGCAGGCTCAATCCGGTTGTCCATGGGGTCATAGAAATCAACCCAGATGCATTGCTGCAAGCTTACAAGGCTGACAGAGAACGAGAGGCTAATAAGCCTGGATCGCTTGGTGGGTTGCACGGAATTCCAATTCTACTCAAGGATAACATTGGGACTAAGGATAAGTTGAATACTACTGCTGGATCATTCGCATTGCTTGGGTCTATCGTGCCTCGTGATGCAGGTGTAGTGAAGAGACTAAGGAGAGCTGGAGCTATCATACTAGGAAAGGCCAGCTTGACTGAATGGGCTGATTTTAGGTCCCTCACTGCTCCAGCTGGTTTGAGCGCTAGAGGTGGTCAAGGAAAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACCGAGACCGATGGTTCTATCCTCTGTCCGGCTAGTTTTAACTCAGTTGTTGGCATCAAACCAACGGTCGGCCTCACTAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATTGGCAGGACAGTGACAGATGCTGTGGTAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGCAGCCACTAGAACAGCCTCTAAGTACATTCCATATGGTGGCTACAAACAATTCCTCAATCCAAATGGGTTGAAAGGGAAGAGATTAGGAATTGTAAGAAACCCCTTTTTCAGCTTCTTCAATGATTCAGCAATCACACAAGCTTTTGAGGATCATTTCAACACTCTAAAGCAAGGCGGTGCTATTTTGATAGACAATCTAGAGATAGCAAATATAGATATCATCTTAAACGTAACGGCAAGTGGAGAAGCAGCAGCATTGCTTGCTGAATTCAAACAATCGTTAAATGGGTACCTGAAAGAGCTTGTGGTTTCCCCTGTTCGAAGTTTAGCCGACATAATCGCCTTCAATAACGCAAATGCAGATCAGGAACTTCTCAACGTTTTCGGCCAGGAGATCTTTCTGGCAGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGGGAAGCTAGCAGAAGATGGGTTTGAGAAATTAGTGAAGGAGAAGCGGTTGGATGCGGTGGTGACGCCAGGTTCCGGCATAGCTACAGTGCTTGCAATTGGGGGTTTACCGGGGATCAATGTTCCGGCGGGATACGATGGCGGAGGAGTTCCGTTTGGGATTAACTTTGGAGGGTTGAAGGGATCAGAGGCGAAGCTGATTGAAGTTGCTTATGGGTTTGAGCAAGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAACCTTAA

Protein sequence

MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP

Homology

[Close]

BLAST of PI0021801.1 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.6e-169
Identity = 319/512 (62.30%), Postives = 397/512 (77.54%), Query Frame = 0


Query: 12  FSLLLILV----AFCLTPPHSVTVR---GLSIREATVHDLQLAFEQNQLTSRELVNFYIG 71
           FS LLIL+    A  ++   +  +R     SI+EAT+ D+++AF + +LTS++LV  Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 72  EIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNT 131
            I +LNP++H VIE NPDAL+QA  ADRER+      L  LHG+P+LLKD+I TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 132 TAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNP 191
           TAGSFALLGS+V RDAGVVKRLR +GA+ILGKASL+EWA FRS + P G SARG QGKNP
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 192 YVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVI 251
           YVLSA+P GSSSG +ISV AN+ AVS+GTETDGSIL PAS NSVVGIKP+VGLTSRAGV+
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 252 PVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGK 311
           P+S RQD+IGPI RTV+DAV +LD IVG+D  D AT+TAS++IP GGYKQFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 312 RLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAAL 371
           RLGIV             +   + H  TL++ GAI+I+NL I NI++I+  T SGE  AL
Sbjct: 309 RLGIVMKH----------SSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 372 LAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQ 431
           LAEFK SLN YLKELV SPVRSLAD+IA+N   A+QE +  +GQE+FL AEAT+G+G+ +
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 432 KAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFG 491
           K AL  + +L+ +G EKL++E +LDA+VT GS +++VLAIGG PGINVPAGYD GGVP+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 488

Query: 492 INFGGLKGSEAKLIEVAYGFEQATLIRKPPSF 517
           I+FGGL+ SE KLIE+A+ FEQATLIRKPP F
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPPKF 510

[Close]

BLAST of PI0021801.1 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 9.4e-79
Identity = 201/515 (39.03%), Postives = 285/515 (55.34%), Query Frame = 0


Query: 32  RGLSIREATVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKAD 91
           + +++ +AT+  LQ   E   LTS ++V+ Y+    ++NP V+G++++NPD L  A + D
Sbjct: 62  QNITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELD 121

Query: 92  REREANKPGSL-GGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAG 151
            ER AN  G + G LHGIP ++KDN  TKDK++TTAGS+ALLGSIVPRDA VVK+LR AG
Sbjct: 122 DER-AN--GIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 181

Query: 152 AIILGKASLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVS 211
           A++ G A+L+EWAD RS     G SARGGQ + P+ L+ +P GSSSG +ISVA+N+ A +
Sbjct: 182 AVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFA 241

Query: 212 IGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTI 271
           +GTETDGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAV V  ++
Sbjct: 242 LGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSM 301

Query: 272 VGFDYNDAATRTASKYIPY-GGYKQFL-NPNGLKGKRLGIVRNPFFSFFNDSAITQAFED 331
            G D ND  T   +   P  G Y +FL N   L+G R G+     +       I +  E 
Sbjct: 302 WGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLE- 361

Query: 332 HFNTLKQGGAILIDNLEIANIDII--------LNVTASGEAAALLAEFKQSLNGYLKELV 391
               +++ GAI+ +N    N+D+I        L      E   +  +F  ++  YL E+ 
Sbjct: 362 VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVK 421

Query: 392 VSPVRSLADIIAFNNANADQE-----LLNVF--GQEIFLAAEATNGI-GDVQKAALLNLG 451
            + + SL DI+ +NN     E     ++  F  GQ+ FLA+    G+  +    A+  + 
Sbjct: 422 NTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVR 481

Query: 452 KLAED-GFE---KLVKEKRLDAVVTPG----SGIATV---LAIGGLPGINVPAGYDGGGV 511
           + ++D G +        K  D+ +  G    SG +      A  G P I +P G    G 
Sbjct: 482 RTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 541

Query: 512 PFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSF 517
           PFG+        E +LI+     E     +  P F
Sbjct: 542 PFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKPKF 572

[Close]

BLAST of PI0021801.1 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.8e-68
Identity = 174/487 (35.73%), Postives = 252/487 (51.75%), Query Frame = 0


Query: 44  LQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLG 103
           LQ  + Q  +    +V  Y+  I  +N  V  V EINPDAL  A + D ER+  K    G
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGK--LRG 153

Query: 104 GLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWA 163
            LHG+PI++K+NI T DK+++TAGS+A+ G+    DA V  +LR AG +I+GK+  ++WA
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 164 DFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPA 223
           +FRSL +  G SA GGQ    Y+ +  P GSSSG  ++    +A  ++GTET GSI+ PA
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 224 SFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTA 283
             +++VG+KPTVGLTSR  V+P+S RQDT+GP+ R+V DA  +L  I G D ND  T +A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 284 SKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDN 343
             +     Y +  + N LKGKR+G+ RN    F +   +   F      +K+ GAI+++N
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 393

Query: 344 LEIANIDIILNVTASGEAAALLAEFKQSLNGYLKELVVSP--VRSLADIIAFNNANADQE 403
            +  +           +   L A+   +L  + K+L V+P  +  L  +  F   +  +E
Sbjct: 394 TDFTSFAEFAQSPIPDD--ILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHHRLEE 453

Query: 404 L--LNVFGQEIFLAAEATNGIGDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIA 463
               +    +I L     N           N+    E G    ++  +LDA V P     
Sbjct: 454 YPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLSP 513

Query: 464 TVLAIGGLPGINVPAGY---------------DGGGVPFGINFGGLKGSEAKLIEVAYGF 512
            + A+ G P I VP G                 G G+P GI F G   SE KLI +AY F
Sbjct: 514 YIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYAF 573

[Close]

BLAST of PI0021801.1 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 5.0e-48
Identity = 157/506 (31.03%), Postives = 247/506 (48.81%), Query Frame = 0


Query: 34  LSIREATVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADRE 93
           + +   T+H+L+   ++ ++++ E+   Y+  I+ + P +  ++ I  D  LQ  KA   
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQ--KAKEA 60

Query: 94  REANKPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAII 153
            E  K G    L GIP+++KDNI T + + TT  S  L   I P +A VV++L   G II
Sbjct: 61  DEKIKKGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVII 120

Query: 154 LGKASLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGT 213
           LGK++L E+A   S    A  +      KNP+ LS  P GSS G + ++AA+ AA ++G+
Sbjct: 121 LGKSNLDEFAMGSSTENSAFKTT-----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGS 180

Query: 214 ETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGF 273
           +T GSI  PAS   VVG+KPT GL SR G++  +   D IGP  + VTD  +VL+TI+G 
Sbjct: 181 DTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGH 240

Query: 274 DYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTL 333
           D  D+ +    K      Y  +L  + +KG R+G+ +  FF    +  + +  ++    L
Sbjct: 241 DPKDSTSLKIDK----PDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVL 300

Query: 334 KQGGAILIDNLEIANIDIIL---NVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADI 393
           +  GA +ID + I  ++  L    + AS EA++ LA +     G++ E          D+
Sbjct: 301 QDLGAEIID-ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAE-------KYEDL 360

Query: 394 IAFNNANADQELLNVFGQEIFLAAEA-TNGIGDVQKAALLNLGKLAEDGFEKLVKEKRLD 453
           I        +       + I L   A ++G  D      L +  L ++ FEK    ++ D
Sbjct: 361 IDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCD 420

Query: 454 AVVTPGS-------------GIATVLA--------IGGLPGINVPAGYDGGGVPFGINFG 513
            ++ P S              +A  LA        I GLPGI++P G    G+P G+   
Sbjct: 421 VIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVGLQII 480

Query: 514 GLKGSEAKLIEVAYGFEQATLIRKPP 515
           G    E K++ VAY FEQA      P
Sbjct: 481 GRHFDEGKILNVAYAFEQANKFSAKP 481

[Close]

BLAST of PI0021801.1 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.3e-47
Identity = 159/510 (31.18%), Postives = 249/510 (48.82%), Query Frame = 0


Query: 34  LSIREATVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADRE 93
           + +   T+H+L+   ++ ++++ E+   Y+  I+ + P +  +I I  D  LQ  +A   
Sbjct: 1   MELYSLTIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQ--RAKEA 60

Query: 94  REANKPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAII 153
            E  K G    L GIP+++KDNI T + + TT  S  L   I P +A VV++L   G II
Sbjct: 61  DEKIKNGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVII 120

Query: 154 LGKASLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGT 213
           LGK++L E+A   S    A  +      KNP+ LS  P GSS G + ++AA+ AA ++G+
Sbjct: 121 LGKSNLDEFAMGSSTENSAFKTT-----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGS 180

Query: 214 ETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGF 273
           +T GSI  PAS   VVG+KPT GL SR G++  +   D IGP  + VTD  +VL+TI+G 
Sbjct: 181 DTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGH 240

Query: 274 DYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTL 333
           D  D+ +    K      Y  +L  + +KG R+G+ +  FF    +  + +  ++    L
Sbjct: 241 DPKDSTSLKIDK----PDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVL 300

Query: 334 KQGGAILIDNLEIANIDIIL---NVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADI 393
           +  GA +ID + I  ++  L    + AS EA++ LA +     G+    +      L D+
Sbjct: 301 QDLGAEIID-ISIPYVEYALPAYYIIASAEASSNLARYDGIRYGH----IAGKYEDLIDM 360

Query: 394 IAFNNANADQELLNVFGQE----IFLAAEA-TNGIGDVQKAALLNLGKLAEDGFEKLVKE 453
                +         FG+E    I L   A ++G  D      L +  L ++ FEK    
Sbjct: 361 YMVTRSEG-------FGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF-- 420

Query: 454 KRLDAVVTPGS-------------GIATVLA--------IGGLPGINVPAGYDGGGVPFG 513
           ++ D ++ P S              +A  LA        I GLPGI++P G    G+P G
Sbjct: 421 EKCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVG 480

Query: 514 INFGGLKGSEAKLIEVAYGFEQATLIRKPP 515
           +   G    E K++ VAY FEQA      P
Sbjct: 481 LQIIGRHFDEGKILNVAYAFEQANKFNAKP 481

[Close]

BLAST of PI0021801.1 vs. ExPASy TrEMBL
Match: A0A0A0K2I0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.0e-277
Identity = 499/518 (96.33%), Postives = 508/518 (98.07%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MADSSP SSVAFSLLLILVAFC TP HSVTVRGLSIREATVHDLQLAF+QNQLTSRELVN
Sbjct: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSL GLHGIP+LLKDNIGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVKRLR+AGAIILGKASL+EWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAFEDHFNTLKQGGAILIDNLEIA+IDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQKAA+LNLGKL EDGFEKLVKE +LDAVVTPG+GIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSE KLIEVAYGFEQATLIRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. ExPASy TrEMBL
Match: A0A5A7U6F5 (Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00780 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.6e-277
Identity = 498/518 (96.14%), Postives = 509/518 (98.26%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MA+SSPLSSVAFSLLLILVAFCLT P+ VTVRGLSIREATVHDLQLAF+QNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSL GLHGIPILLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASL+EWADFRSLTAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEIANI+IILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQKAALLNLGKL EDGFEKLVKE +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSEAKLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. ExPASy TrEMBL
Match: A0A1S4DXK2 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 8.6e-277
Identity = 497/518 (95.95%), Postives = 509/518 (98.26%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MA+SSPLSSVAFS+LLILVAFCLT P+ VTVRGLSIREATVHDLQLAF+QNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSL GLHGIPILLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASL+EWADFRSLTAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEIANIDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQKAALLNLGKL EDGFEKLVK+ +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSEAKLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. ExPASy TrEMBL
Match: A0A6J1HUS8 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 4.4e-257
Identity = 465/511 (91.00%), Postives = 482/511 (94.32%), Query Frame = 0


Query: 8   SSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVNFYIGEIR 67
           S    S LLI+VAFCLT P S  VRG SIREATV DL LAF++N+LTSR LV FYIGEIR
Sbjct: 16  SMACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIR 75

Query: 68  RLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNTTAG 127
           RLNPVVHGVIEINPDALLQAYKADREREA KPGSL GLHGIP+LLKD+IGTKDKLNTTAG
Sbjct: 76  RLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAG 135

Query: 128 SFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNPYVL 187
           SFALLGSIVPRDAGVVKRLRRAGAIILGKASL+EWADFRSL APAGLSARGGQGKNPYVL
Sbjct: 136 SFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL 195

Query: 188 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 247
           SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS
Sbjct: 196 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 255

Query: 248 PRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLG 307
           PRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRT+SKYIPYGGYKQFLN NGLKGKRLG
Sbjct: 256 PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLG 315

Query: 308 IVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAE 367
           IVRNPFFSFFNDSAITQAFEDHFN LKQGGA+L+DNLEIANIDIILNVTASGEAAALLAE
Sbjct: 316 IVRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAE 375

Query: 368 FKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAA 427
           FKQSLN YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA
Sbjct: 376 FKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAA 435

Query: 428 LLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINF 487
           LLNL KL +DGFEK+V+E+RLDAVVTPG  IATVLAIGG PGINVPAGYDGGGVPFGINF
Sbjct: 436 LLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINF 495

Query: 488 GGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           GGLKGSE KLIEVAY FEQATLIRKPPSFKP
Sbjct: 496 GGLKGSEPKLIEVAYAFEQATLIRKPPSFKP 526

[Close]

BLAST of PI0021801.1 vs. ExPASy TrEMBL
Match: A0A6J1GLV2 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.4e-255
Identity = 462/506 (91.30%), Postives = 478/506 (94.47%), Query Frame = 0


Query: 13  SLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPV 72
           S LLI+VAFCLT P S  VRG SIREATV DLQLAF++N+LTSR LV FYIGEIRRLNPV
Sbjct: 5   SWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPV 64

Query: 73  VHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALL 132
           VHGVIEINPDALLQAYKADREREA KPGSL GLHGIP+LLKD+IGTKDKLNTTAGSFALL
Sbjct: 65  VHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124

Query: 133 GSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPC 192
           GSIVPRDAGVVKRLRRAGAIILGKASL+EWADFRSL APAGLSARGGQGKNPYVLSASPC
Sbjct: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPC 184

Query: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252
           GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 185 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244

Query: 253 IGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312
           IGPIGRTVTDAV+VLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304

Query: 313 FFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAEFKQSL 372
           FFSFFNDSAITQAFEDHFN LKQGGAIL+DNLEI NIDIILNVTASGEA ALLAEFKQSL
Sbjct: 305 FFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSL 364

Query: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAALLNLG 432
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAALLNL 
Sbjct: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424

Query: 433 KLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINFGGLKG 492
           KL +DGFEK+V+E+RLDAVVTPG  IATVLAIGG PGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 484

Query: 493 SEAKLIEVAYGFEQATLIRKPPSFKP 519
           SE  LIEVAY FEQATLIRKPPSFKP
Sbjct: 485 SEPTLIEVAYAFEQATLIRKPPSFKP 510

[Close]

BLAST of PI0021801.1 vs. NCBI nr
Match: XP_031745278.1 (probable amidase At4g34880 [Cucumis sativus] >KGN43234.1 hypothetical protein Csa_020255 [Cucumis sativus])

HSP 1 Score: 965.3 bits (2494), Expect = 2.1e-277
Identity = 499/518 (96.33%), Postives = 508/518 (98.07%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MADSSP SSVAFSLLLILVAFC TP HSVTVRGLSIREATVHDLQLAF+QNQLTSRELVN
Sbjct: 1   MADSSPFSSVAFSLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSL GLHGIP+LLKDNIGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAGVVKRLR+AGAIILGKASL+EWADFRSL APAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAFEDHFNTLKQGGAILIDNLEIA+IDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQKAA+LNLGKL EDGFEKLVKE +LDAVVTPG+GIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAAVLNLGKLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSE KLIEVAYGFEQATLIRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSETKLIEVAYGFEQATLIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. NCBI nr
Match: KAA0049736.1 (putative amidase isoform X2 [Cucumis melo var. makuwa] >TYK12141.1 putative amidase isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 962.2 bits (2486), Expect = 1.8e-276
Identity = 498/518 (96.14%), Postives = 509/518 (98.26%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MA+SSPLSSVAFSLLLILVAFCLT P+ VTVRGLSIREATVHDLQLAF+QNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSL GLHGIPILLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASL+EWADFRSLTAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEIANI+IILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQKAALLNLGKL EDGFEKLVKE +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSEAKLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. NCBI nr
Match: XP_016900445.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 962.2 bits (2486), Expect = 1.8e-276
Identity = 497/518 (95.95%), Postives = 509/518 (98.26%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MA+SSPLSSVAFS+LLILVAFCLT P+ VTVRGLSIREATVHDLQLAF+QNQLTSRELVN
Sbjct: 1   MANSSPLSSVAFSMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVN 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEIRRLNPVVHGVIEINPDAL+QAYKADREREANKPGSL GLHGIPILLKD IGTKD
Sbjct: 61  FYIGEIRRLNPVVHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVPRDAG+VKRLR+AGAIILGKASL+EWADFRSLTAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDS ITQAF+DHFNTLKQGGAILIDNLEIANIDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQKAALLNLGKL EDGFEKLVK+ +LDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQKAALLNLGKLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSEAKLIEVAYGFEQAT IRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATQIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. NCBI nr
Match: XP_038887424.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 933.7 bits (2412), Expect = 6.8e-268
Identity = 486/518 (93.82%), Postives = 495/518 (95.56%), Query Frame = 0


Query: 1   MADSSPLSSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVN 60
           MA SSPLSSVAFSLLLILVAFCLT PHS TVRGLSIREATV DLQLAF+QNQLTSR LV 
Sbjct: 1   MAYSSPLSSVAFSLLLILVAFCLTAPHSATVRGLSIREATVQDLQLAFKQNQLTSRRLVE 60

Query: 61  FYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKD 120
           FYIGEI RLNPVVHGVIEINPDALLQAYKADRER A KPGSL GLHGIP+LLKDNIGTKD
Sbjct: 61  FYIGEIHRLNPVVHGVIEINPDALLQAYKADRERAAKKPGSLCGLHGIPVLLKDNIGTKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQ 180
           KLNTTAGSFALLGSIVP DAG+VKRLRRAGAIILGKASL+EWADFRSLTAPAGLSARGGQ
Sbjct: 121 KLNTTAGSFALLGSIVPHDAGIVKRLRRAGAIILGKASLSEWADFRSLTAPAGLSARGGQ 180

Query: 181 GKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240
           GKNPYVLSASPCGSSSGP+ISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR
Sbjct: 181 GKNPYVLSASPCGSSSGPAISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSR 240

Query: 241 AGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNG 300
           AGVIPVSPRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRT SKYIP GGYKQFLNP G
Sbjct: 241 AGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTTSKYIPNGGYKQFLNPIG 300

Query: 301 LKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGE 360
           LKGKRLGIVRNPFFSFFNDSAIT+AFEDHFNTLKQGGAILIDNLEI NIDIILNVTASGE
Sbjct: 301 LKGKRLGIVRNPFFSFFNDSAITEAFEDHFNTLKQGGAILIDNLEIENIDIILNVTASGE 360

Query: 361 AAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGI 420
           A ALLAEFKQSLN YLKELV SPVRSLADIIAFNNAN DQELLNVFGQEIFLAAEATNGI
Sbjct: 361 AVALLAEFKQSLNEYLKELVASPVRSLADIIAFNNANPDQELLNVFGQEIFLAAEATNGI 420

Query: 421 GDVQKAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGG 480
           GDVQ AALLNL KL EDGFEK+VK+KRLDAVVTPGSGIATVLAIGG PGINVPAGYDGGG
Sbjct: 421 GDVQTAALLNLAKLTEDGFEKVVKKKRLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGG 480

Query: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP
Sbjct: 481 VPFGINFGGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 518

[Close]

BLAST of PI0021801.1 vs. NCBI nr
Match: XP_022968892.1 (putative amidase C869.01 [Cucurbita maxima])

HSP 1 Score: 896.7 bits (2316), Expect = 9.2e-257
Identity = 465/511 (91.00%), Postives = 482/511 (94.32%), Query Frame = 0


Query: 8   SSVAFSLLLILVAFCLTPPHSVTVRGLSIREATVHDLQLAFEQNQLTSRELVNFYIGEIR 67
           S    S LLI+VAFCLT P S  VRG SIREATV DL LAF++N+LTSR LV FYIGEIR
Sbjct: 16  SMACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIR 75

Query: 68  RLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNTTAG 127
           RLNPVVHGVIEINPDALLQAYKADREREA KPGSL GLHGIP+LLKD+IGTKDKLNTTAG
Sbjct: 76  RLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAG 135

Query: 128 SFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNPYVL 187
           SFALLGSIVPRDAGVVKRLRRAGAIILGKASL+EWADFRSL APAGLSARGGQGKNPYVL
Sbjct: 136 SFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVL 195

Query: 188 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 247
           SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS
Sbjct: 196 SASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVS 255

Query: 248 PRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLG 307
           PRQDTIGPIGRTVTDAV+VLDTIVGFDYNDAATRT+SKYIPYGGYKQFLN NGLKGKRLG
Sbjct: 256 PRQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLG 315

Query: 308 IVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAE 367
           IVRNPFFSFFNDSAITQAFEDHFN LKQGGA+L+DNLEIANIDIILNVTASGEAAALLAE
Sbjct: 316 IVRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAE 375

Query: 368 FKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAA 427
           FKQSLN YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA
Sbjct: 376 FKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAA 435

Query: 428 LLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFGINF 487
           LLNL KL +DGFEK+V+E+RLDAVVTPG  IATVLAIGG PGINVPAGYDGGGVPFGINF
Sbjct: 436 LLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINF 495

Query: 488 GGLKGSEAKLIEVAYGFEQATLIRKPPSFKP 519
           GGLKGSE KLIEVAY FEQATLIRKPPSFKP
Sbjct: 496 GGLKGSEPKLIEVAYAFEQATLIRKPPSFKP 526

[Close]

BLAST of PI0021801.1 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.3e-144
Identity = 282/512 (55.08%), Postives = 356/512 (69.53%), Query Frame = 0


Query: 12  FSLLLILV----AFCLTPPHSVTVR---GLSIREATVHDLQLAFEQNQLTSRELVNFYIG 71
           FS LLIL+    A  ++   +  +R     SI+EAT+ D+++AF + +LTS++LV  Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 72  EIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNT 131
            I +LNP++H VIE NPDAL+QA  ADRER+      L  LHG+P+LLKD+I TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 132 TAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNP 191
           TAGSFALLGS+V RDAGVVKRLR +GA+ILGKASL+EWA FRS + P G SA        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 192 YVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVI 251
                                                  S NSVVGIKP+VGLTSRAGV+
Sbjct: 189 --------------------------------------PSQNSVVGIKPSVGLTSRAGVV 248

Query: 252 PVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGK 311
           P+S RQD+IGPI RTV+DAV +LD IVG+D  D AT+TAS++IP GGYKQFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 312 RLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAAL 371
           RLGIV             +   + H  TL++ GAI+I+NL I NI++I+  T SGE  AL
Sbjct: 309 RLGIVMKH----------SSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 372 LAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQ 431
           LAEFK SLN YLKELV SPVRSLAD+IA+N   A+QE +  +GQE+FL AEAT+G+G+ +
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 432 KAALLNLGKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIGGLPGINVPAGYDGGGVPFG 491
           K AL  + +L+ +G EKL++E +LDA+VT GS +++VLAIGG PGINVPAGYD GGVP+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 464

Query: 492 INFGGLKGSEAKLIEVAYGFEQATLIRKPPSF 517
           I+FGGL+ SE KLIE+A+ FEQATLIRKPP F
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPPKF 464

[Close]

BLAST of PI0021801.1 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.7e-30
Identity = 144/493 (29.21%), Postives = 229/493 (46.45%), Query Frame = 0


Query: 52  QLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREANKPGSLGGLHGIPIL 111
           + T+ E+   Y+  IR   P +   + ++ + L  A + D  +   K   LG L G+ I 
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID--QRIAKGEELGPLAGVLIG 116

Query: 112 LKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLTEWADFRSLTAP 171
           +KDNI T+  + +TA S  L     P DA  VK+++  G I++GK ++ E+    S T  
Sbjct: 117 VKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFG-MGSTTEA 176

Query: 172 AGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGI 231
           +          NP+ LS  P GSS G + +VAA    VS+G++T GS+  PASF  VVG+
Sbjct: 177 SAFQVTA----NPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGL 236

Query: 232 KPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYNDAATRTASKYIPYGG 291
           KPT G  SR G++  +   D IG  G TV DA ++L  I G+D  D+   T+SK      
Sbjct: 237 KPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDS---TSSKQDVPEF 296

Query: 292 YKQFLN-----PNGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNTLKQGGAILIDNLEI 351
             QFL+        L G ++GI+R        DS +  A ++  + L+  G IL + + +
Sbjct: 297 QSQFLSVDHFESKPLNGVKVGIIRETLEDGV-DSGVRSATQEAASHLEALGCILTE-VSL 356

Query: 352 ANIDIIL---NVTASGEAAALLAEFKQSLNGYLKELVVSPVRSLADIIAFNNANADQELL 411
            +  + L    V AS E+++ L+ +     G   +++   +  L +         + ++ 
Sbjct: 357 PSFSLGLPAYYVIASSESSSNLSRYDGVRYG--NQVMAEELNKLYECSRGEGFGGEVKMR 416

Query: 412 NVFGQEIFLAA----------EATNGIGDVQKAAL----LNLGKLAEDGFEKLVKEKRLD 471
            + G     A           +    I    KAAL    + +   A     K + EK+ D
Sbjct: 417 ILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYK-IGEKKDD 476

Query: 472 AVVTPGSGIATV-LAIGGLPGINVPAG-YDGG--GVPFGINFGGLKGSEAKLIEVAYGFE 519
            +      I TV + + GLP + +P G  +GG  G+P G+   G    E KL++V + FE
Sbjct: 477 PLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFE 530

[Close]

BLAST of PI0021801.1 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-26
Identity = 81/252 (32.14%), Postives = 133/252 (52.78%), Query Frame = 0


Query: 40  TVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADRERE--AN 99
           +V +L    +  Q+TS+ELV  Y+ +++R N V+  V+    +    AYK  +E +   +
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL---AYKQAKEADDLLS 263

Query: 100 KPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 159
           +   LG LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 160 SLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDG 219
            +T    +  +         GG+ +NP+ +     GSS+GP+ S +A +   +IG+ET G
Sbjct: 324 -VTGSMAYDDIWF-------GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAG 383

Query: 220 SILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYND 279
           S+  PA+   +  ++PT G   R GV+ +S   D +GP  RT  D  V+LD I G D +D
Sbjct: 384 SMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 443

Query: 280 AATRTASKYIPY 290
            ++R  +   P+
Sbjct: 444 LSSREIAFEDPF 443

[Close]

BLAST of PI0021801.1 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.3e-24
Identity = 80/252 (31.75%), Postives = 130/252 (51.59%), Query Frame = 0


Query: 40  TVHDLQLAFEQNQLTSRELVNFYIGEIRRLNPVVHGVIEINPDALLQAYKADRERE--AN 99
           +V +L    +  Q+TS+ELV  Y+ +++R N V+  V+    +    AYK  +E +   +
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL---AYKQAKEADDLLS 263

Query: 100 KPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 159
           +   LG LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 160 SLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSISVAANIAAVSIGTETDG 219
            +T    +  +         GG+ +NP+ +     GSS+GP+       A+ S G+ET G
Sbjct: 324 -VTGSMAYDDIWF-------GGRTRNPWNIEEFSTGSSAGPA-------ASTSAGSETAG 383

Query: 220 SILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVVVLDTIVGFDYND 279
           S+  PA+   +  ++PT G   R GV+ +S   D +GP  RT  D  V+LD I G D +D
Sbjct: 384 SMTYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 436

Query: 280 AATRTASKYIPY 290
            ++R  +   P+
Sbjct: 444 LSSREIAFEDPF 436

[Close]

BLAST of PI0021801.1 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 94.0 bits (232), Expect = 3.9e-19
Identity = 110/446 (24.66%), Postives = 190/446 (42.60%), Query Frame = 0


Query: 82  DALLQAYKADREREANKPGSLGGLHGIPILLKDNIGTKDKLNTTAGSFALLGSIVPRDAG 141
           + + QA  + R  E   P S+  L GI + +KD+I           ++      V +D+ 
Sbjct: 176 EVIKQAEASTRRFEQGNPISV--LDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSA 235

Query: 142 VVKRLRRAGAIILGKASLTEWADFRSLTAPAGLSARGGQGKNPYVLSASPCGSSSGPSIS 201
           VV +LR  GAI+LGKA++ E           G ++  G  +NP+       GSSSG +  
Sbjct: 236 VVSKLRSCGAILLGKANMHELG-----MGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAI 295

Query: 202 VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVT 261
           VAA + + ++GT+  GS+  P++   + G+K T G T   G +      + IGP+  ++ 
Sbjct: 296 VAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLE 355

Query: 262 DAVVVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVR-NPFFSFFNDS 321
           DA +V   I+G   + +A R   K  P   + + L+ NG     +G +R   +  +FND 
Sbjct: 356 DAFLVYAAILG---SSSADRYNLKPSP-PCFPKLLSHNG--SNAIGSLRLGKYTKWFNDV 415

Query: 322 AITQAFEDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAEFKQSLNGYLKELV 381
           + +   +   + LK    +L +N     ++I++       AA +++    +L+       
Sbjct: 416 SSSDISDKCEDILK----LLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCE 475

Query: 382 VSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEA---------TNGIGDVQKAALLNL 441
                 L+      +      +   F    ++AA+           N   DV        
Sbjct: 476 AGKNSKLS-----YDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTT 535

Query: 442 GKLAEDGFEKLVKEKRLDAVVTPGSGIATVLAIG--GLPGINVPAGYDGGGVPFGINFGG 501
           G  A       +K    +  VT    +  VLA    G P I+VP GYD  G+P G+   G
Sbjct: 536 GMTAPVIPPDALKNGETNIQVTTDL-MRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMG 595

Query: 502 LKGSEAKLIEVAYGFEQATLIRKPPS 516
              +EA ++ +A   E+   + K P+
Sbjct: 596 RPWAEATVLGLAAAVEELAPVTKKPA 598

The following BLAST results are available for this feature:

BLAST of PI0021801.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1P8B760	2.6e-169	62.30	Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=...	[more]
Q9URY4	9.4e-79	39.03	Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
D4B3C8	9.8e-68	35.73	Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ...	[more]
B0K3S3	5.0e-48	31.03	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ...	[more]
B0KBN4	3.3e-47	31.18	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol...	[more]

BLAST of PI0021801.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2I0	1.0e-277	96.33	Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=...	[more]
A0A5A7U6F5	8.6e-277	96.14	Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S4DXK2	8.6e-277	95.95	putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4...	[more]
A0A6J1HUS8	4.4e-257	91.00	putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1	[more]
A0A6J1GLV2	1.4e-255	91.30	putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1	[more]

BLAST of PI0021801.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031745278.1	2.1e-277	96.33	probable amidase At4g34880 [Cucumis sativus] >KGN43234.1 hypothetical protein Cs...	[more]
KAA0049736.1	1.8e-276	96.14	putative amidase isoform X2 [Cucumis melo var. makuwa] >TYK12141.1 putative amid...	[more]
XP_016900445.1	1.8e-276	95.95	PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo]	[more]
XP_038887424.1	6.8e-268	93.82	probable amidase At4g34880 [Benincasa hispida]	[more]
XP_022968892.1	9.2e-257	91.00	putative amidase C869.01 [Cucurbita maxima]	[more]

BLAST of PI0021801.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34880.1	2.3e-144	55.08	Amidase family protein	[more]
AT3G25660.1	1.7e-30	29.21	Amidase family protein	[more]
AT5G07360.1	1.1e-26	32.14	Amidase family protein	[more]
AT5G07360.2	2.3e-24	31.75	Amidase family protein	[more]
AT5G64440.1	3.9e-19	24.66	fatty acid amide hydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023631	Amidase signature domain	PFAM	PF01425	Amidase	coord: 57..459 e-value: 1.8E-69 score: 234.9
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 24..516 e-value: 1.0E-143 score: 482.0
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 35..515
None	No IPR available	PANTHER	PTHR42678:SF19	C869.01-LIKE PROTEIN, PUTATIVE-RELATED	coord: 29..518
None	No IPR available	PANTHER	PTHR42678	AMIDASE	coord: 29..518

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0021801	PI0021801	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0021801.1-exon	PI0021801.1-exon-chr01:5369174..5369530	exon
PI0021801.1-exon	PI0021801.1-exon-chr01:5369609..5369807	exon
PI0021801.1-exon	PI0021801.1-exon-chr01:5369942..5370181	exon
PI0021801.1-exon	PI0021801.1-exon-chr01:5370267..5370481	exon
PI0021801.1-exon	PI0021801.1-exon-chr01:5370580..5371125	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0021801.1-cds	PI0021801.1-cds-chr01:5369174..5369530	CDS
PI0021801.1-cds	PI0021801.1-cds-chr01:5369609..5369807	CDS
PI0021801.1-cds	PI0021801.1-cds-chr01:5369942..5370181	CDS
PI0021801.1-cds	PI0021801.1-cds-chr01:5370267..5370481	CDS
PI0021801.1-cds	PI0021801.1-cds-chr01:5370580..5371125	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0021801.1	PI0021801.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane