PI0016610.1 (mRNA) Melon (PI 482460) v1

BLAST of PI0016610.1 vs. ExPASy Swiss-Prot
Match: P0CI03 (Protein NRT1/ PTR FAMILY 5.6 OS=Arabidopsis thaliana OX=3702 GN=NPF5.6 PE=2 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.6e-212
Identity = 362/556 (65.11%), Postives = 444/556 (79.86%), Query Frame = 0

Query: 17  EDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIH 76
           +D Q WV DSS+D +G +PLRA TG W+++LFIIAIEFSERLSYFG+AT+LV+YLT +++
Sbjct: 16  DDQQKWVLDSSLDSRGRVPLRARTGAWRAALFIIAIEFSERLSYFGLATNLVVYLTTILN 75

Query: 77  EDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPS 136
           +DLK A RNVNYW+GVTTLMPLLGGF+ADAYLGR++TVL++T IYL+GL LLTMS  +P 
Sbjct: 76  QDLKMAIRNVNYWSGVTTLMPLLGGFIADAYLGRYATVLVATTIYLMGLVLLTMSWFIPG 135

Query: 137 LKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMSF 196
           LKPC  E C EPRK HE+ F+ AIYLISIGTGGHKPSLESFGADQFDD H EERK KMSF
Sbjct: 136 LKPCHQEVCVEPRKAHEVAFFIAIYLISIGTGGHKPSLESFGADQFDDDHVEERKMKMSF 195

Query: 197 FNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPLG 256
           FNWWN  LCAG++  VT + Y+++ VGWG+ G+ILT VMAISL IF +G+P YR+R P G
Sbjct: 196 FNWWNVSLCAGILTAVTAVAYIEDRVGWGVAGIILTVVMAISLIIFFIGKPFYRYRTPSG 255

Query: 257 SPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFLDKAAIIEE 316
           SPLTP+LQV VAA  KR L YPS  S L+E V  ++   GRLL HT++LKFLDKAAIIE+
Sbjct: 256 SPLTPILQVFVAAIAKRNLPYPSDPSLLHE-VSKTEFTSGRLLCHTEHLKFLDKAAIIED 315

Query: 317 RGN-SKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLDRE 376
           +   + EKQ  WRL T+T+VEE KLI+N+IPIW ++L FGIC  QASTFF+KQ  T+DR 
Sbjct: 316 KNPLALEKQSPWRLLTLTKVEETKLIINVIPIWFSTLAFGICATQASTFFIKQAITMDRH 375

Query: 377 IRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFSFT 436
           I   F +P +SMF L A  +I+S+ +Y+KLLVPLLR  T N+RGI+ILQRIG GM+FS  
Sbjct: 376 I-GGFTVPPASMFTLTALTLIISLTVYEKLLVPLLRSITRNQRGINILQRIGTGMIFSLI 435

Query: 437 TMVVAALVERKRLGQTTH--PMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMRSL 496
           TM++AALVE++RL +T +  PMSV WLAPQF +IG  D F LVGLQEYFY QVPDSMRSL
Sbjct: 436 TMIIAALVEKQRLDRTNNNKPMSVIWLAPQFMVIGFADAFTLVGLQEYFYHQVPDSMRSL 495

Query: 497 GIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYWLIAGIVAVDL 556
           GIAFYLSV GAA+FL++ LIT VD + +  S KSWFG DLNSSRLD FYW +AG++A ++
Sbjct: 496 GIAFYLSVIGAASFLNNLLITAVDTLAENFSGKSWFGKDLNSSRLDRFYWFLAGVIAANI 555

Query: 557 CFYVFLARRYTYKSVQ 570
           C +V +A+R  YKSVQ
Sbjct: 556 CVFVIVAKRCPYKSVQ 569

BLAST of PI0016610.1 vs. ExPASy Swiss-Prot
Match: Q9M331 (Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2)

HSP 1 Score: 733.0 bits (1891), Expect = 2.6e-210
Identity = 362/584 (61.99%), Postives = 457/584 (78.25%), Query Frame = 0

Query: 4   NNKLEKIKRDQLEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 63
           +NK++   +D   +D Q WV DSS D +G +PLRA TG W+++LFII IEFSERLSYFGI
Sbjct: 3   HNKVDTEPQDSY-DDQQKWVLDSSTDSRGEIPLRAQTGAWRAALFIIGIEFSERLSYFGI 62

Query: 64  ATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLL 123
           +T+LV+YLT ++H+DLK A +N NYW+GVTTLMPLLGGF+ADAYLGR+ TVLL+T IYL+
Sbjct: 63  STNLVVYLTTILHQDLKMAVKNTNYWSGVTTLMPLLGGFVADAYLGRYGTVLLATTIYLM 122

Query: 124 GLSLLTMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFD 183
           GL LLT+S  +P LK C  + C EPRK HEI F+ AIYLISIGTGGHKPSLESFGADQF+
Sbjct: 123 GLILLTLSWFIPGLKACHEDMCVEPRKAHEIAFFIAIYLISIGTGGHKPSLESFGADQFE 182

Query: 184 DHHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFL 243
           D HPEERK KMS+FNWWN+GLCAG++  VT+IVY+++ +GWG+  +ILT VMA S  IF 
Sbjct: 183 DGHPEERKMKMSYFNWWNAGLCAGILTAVTVIVYIEDRIGWGVASIILTIVMATSFFIFR 242

Query: 244 LGRPVYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTK 303
           +G+P YR+RAP GSPLTP+LQV VAA  KR L  PS SS L+EL  + +  +GRLLS +K
Sbjct: 243 IGKPFYRYRAPSGSPLTPMLQVFVAAIAKRNLPCPSDSSLLHELT-NEEYTKGRLLSSSK 302

Query: 304 NLKFLDKAAIIEERG-NSK-EKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQA 363
           NLKFLDKAA+IE+R  N+K EKQ  WRLATVT+VEE+KL++NMIPIW  +L FG+C  Q+
Sbjct: 303 NLKFLDKAAVIEDRNENTKAEKQSPWRLATVTKVEEVKLLINMIPIWFFTLAFGVCATQS 362

Query: 364 STFFVKQCGTLDREIR-NNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGI 423
           ST F+KQ   +DR I   +F++P +S+F L A  +I++V IY+KLLVPLLR+ TGNERGI
Sbjct: 363 STLFIKQAIIMDRHITGTSFIVPPASLFSLIALSIIITVTIYEKLLVPLLRRATGNERGI 422

Query: 424 SILQRIGIGMVFSFTTMVVAALVERKRLG-------QTTHPMSVFWLAPQFFIIGIGDGF 483
           SILQRIG+GMVFS   M++AAL+E+KRL          T  +S  WLAPQF ++G+ D F
Sbjct: 423 SILQRIGVGMVFSLFAMIIAALIEKKRLDYAKEHHMNKTMTLSAIWLAPQFLVLGVADAF 482

Query: 484 ALVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDL 543
            LVGLQEYFYDQVPDSMRSLGIAFYLSV GAA+F+++ LIT+ D + ++ S K WFG DL
Sbjct: 483 TLVGLQEYFYDQVPDSMRSLGIAFYLSVLGAASFVNNLLITVSDHLAEEISGKGWFGKDL 542

Query: 544 NSSRLDNFYWLIAGIVAVDLCFYVFLARRYTYKSVQKT--TVAD 576
           NSSRLD FYW++A + A ++C +V +A RYTYK+VQ +   VAD
Sbjct: 543 NSSRLDRFYWMLAALTAANICCFVIVAMRYTYKTVQPSLAVVAD 584

BLAST of PI0016610.1 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 454.5 bits (1168), Expect = 1.8e-126
Identity = 242/568 (42.61%), Postives = 351/568 (61.80%), Query Frame = 0

Query: 17  EDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIH 76
           E  +++  D +VD +G   L + TG W++  F++  E  ER++++GIA++LV YLT+ +H
Sbjct: 2   EAAKVYTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLH 61

Query: 77  EDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPS 136
           ED  ++ RNVN W+G   + P+ G ++AD+Y+GRF T   S++IY+LG+ LLTM+  V S
Sbjct: 62  EDTISSVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKS 121

Query: 137 LKP-CDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMS 196
           L+P C++  C +   +    FY ++Y I+IG GG KP++ +FGADQFD +  EE+KQK+S
Sbjct: 122 LRPTCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVS 181

Query: 197 FFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPL 256
           FFNWW      G +F    +VY+QE++GWG+G  I T  + +SL +F +G P YR +   
Sbjct: 182 FFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIK 241

Query: 257 GSPLT-PLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGR-LLSHTKNLKFLDKAAI 316
              L   L+QV +AAF+ RKL  P    +LYEL     K  G+  + HT   +FLDKAAI
Sbjct: 242 TDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI 301

Query: 317 IEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLD 376
                    K  +    TVT+VE  K +L +I IW+ +L      AQ +T FVKQ  TLD
Sbjct: 302 ---------KTSSRVPCTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLD 361

Query: 377 REIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFS 436
           R+I +NF IPA+S+       M+LSV +YD+  VP +RK TGN RGI++LQR+G+G    
Sbjct: 362 RKIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQ 421

Query: 437 FTTMVVAALVERKRLGQTTH----------PMSVFWLAPQFFIIGIGDGFALVGLQEYFY 496
              + +A+ VE KR+               PMS+FWL PQ+ ++GIGD F  +GL E+FY
Sbjct: 422 IVAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFY 481

Query: 497 DQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYW 556
           DQ P+ M+SLG  F+ S  G  NFL+SFL+T++D IT K   KSW G++LN SRLD +Y 
Sbjct: 482 DQSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYG 541

Query: 557 LIAGIVAVDLCFYVFLARRYTYKSVQKT 572
            +  I  V++  +V+ A +Y YKS   T
Sbjct: 542 FLVVISIVNMGLFVWAASKYVYKSDDDT 560

BLAST of PI0016610.1 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 4.4e-125
Identity = 238/571 (41.68%), Postives = 348/571 (60.95%), Query Frame = 0

Query: 10  IKRDQLEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVI 69
           I+   + ++ +++  D SVD  GN PL+  TG WK+  FI+  E  ERL+Y+GIA +L+ 
Sbjct: 12  IEEGLILQEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLIT 71

Query: 70  YLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLT 129
           YLT  +H+   +AA NV  W G   L PL+G  LADAY GR+ T+   + IY +G+S LT
Sbjct: 72  YLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALT 131

Query: 130 MSTLVPSLKP--CDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHP 189
           +S  VP+LKP  C  + C         +F+  +YLI++GTGG KP + SFGADQFDD   
Sbjct: 132 LSASVPALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDS 191

Query: 190 EERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRP 249
            ER +K SFFNW+   +  G +   +L+V++QE+ GWG+G  I T  M +++A F  G P
Sbjct: 192 RERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTP 251

Query: 250 VYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQG-RLLSHTKNLK 309
           +YRF+ P GSP+T + QV+VA+FRK  +  P  ++ LYE    +    G R + HT + +
Sbjct: 252 LYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQ 311

Query: 310 FLDKAAII-EERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFF 369
           +LDKAA+I EE   S +   +WRL TVT+VEELK+++ M PIW + + F    AQ ST F
Sbjct: 312 YLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMF 371

Query: 370 VKQCGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQR 429
           V+Q   ++ +I  +F +P +++     A +I+ V +YD+ +VPL RK TG ++G + +QR
Sbjct: 372 VQQGRAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQR 431

Query: 430 IGIGMVFSFTTMVVAALVERKRLGQTTH----------PMSVFWLAPQFFIIGIGDGFAL 489
           +GIG+  S   M  AA+VE  RL               P+SV W  PQ+FI+G  + F  
Sbjct: 432 MGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYF 491

Query: 490 VGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNS 549
           +G  E+FYDQ PD+MRSL  A  L  N   N+LSS ++T+V + T ++  + W  D+LNS
Sbjct: 492 IGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNS 551

Query: 550 SRLDNFYWLIAGIVAVDLCFYVFLARRYTYK 567
             LD F+WL+AG+  V++  Y F A RY  K
Sbjct: 552 GHLDYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of PI0016610.1 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 1.1e-123
Identity = 238/570 (41.75%), Postives = 348/570 (61.05%), Query Frame = 0

Query: 15  LEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRV 74
           +E+D  I+  D ++D       +  TG WK+  FI+  E  ERL+Y+G++T+L+ YL + 
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 75  IHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLV 134
           ++ +  +A+++V+ W+G     PL+G F+ADAYLGR+ T+    +IY+ G++LLT+S  V
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 135 PSLKP-CDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQK 194
           P L P C  ETC        I F  A+YLI++GTGG KP + SFGADQFDD   +E++ K
Sbjct: 121 PGLTPTCSGETCHATAGQTAITF-IALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 180

Query: 195 MSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRA 254
            SFFNW+   +  G +   +++V++Q +VGWG G  + T  MAI++  F  G   YR + 
Sbjct: 181 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 240

Query: 255 PLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQG-RLLSHTKNLKFLDKAA 314
           P GSPLT +LQVIVA+ RK K+  P   S LYE   +     G R L HTK L F DKAA
Sbjct: 241 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAA 300

Query: 315 IIEERGN-SKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGT 374
           +  E  N    K  +W+L TVT+VEELK ++ ++PIW T + F    +Q  T FV Q  T
Sbjct: 301 VETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNT 360

Query: 375 LDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMV 434
           LD+ +  NF IP++S+       ++    +YDKL+VP  RK TG+ERG + LQRIGIG+V
Sbjct: 361 LDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLV 420

Query: 435 FSFTTMVVAALVERKRL---------GQTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYF 494
            S  +MV A ++E  RL          + T PM++FW  PQ+F++G  + F  +G  E+F
Sbjct: 421 ISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFF 480

Query: 495 YDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFY 554
           YDQ PD+MRSL  A  L+     N+LS+FL+T+V  +T+      W   +LN+  LD F+
Sbjct: 481 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFF 540

Query: 555 WLIAGIVAVDLCFYVFLARRYTYKSVQKTT 573
           WL+AG+  ++   Y+++A+ YTYK   KTT
Sbjct: 541 WLLAGLSFLNFLVYLWIAKWYTYK---KTT 566

BLAST of PI0016610.1 vs. ExPASy TrEMBL
Match: A0A1S3B6K9 (protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo OX=3656 GN=LOC103486351 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 548/589 (93.04%), Postives = 567/589 (96.26%), Query Frame = 0

Query: 1   MKTNNKLEKIKRDQL-EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS 60
           MKTNN+LEK+KRDQL EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS
Sbjct: 1   MKTNNQLEKMKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS 60

Query: 61  YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTI 120
           YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL+ST+
Sbjct: 61  YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTV 120

Query: 121 IYLLGLSLLTMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGA 180
           IYLLGLSLLTMSTLVPSLKPCD ETC+EPRKVHEILF+TAIYLISIGTGGHKPSLESFGA
Sbjct: 121 IYLLGLSLLTMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGA 180

Query: 181 DQFDDHHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL 240
           DQFDD HPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL
Sbjct: 181 DQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL 240

Query: 241 AIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLL 300
           AIFLLGRPVYR+RAP GSPLTPLLQVI+AAFRKRKL YP HSSQL+E VQS DKFQGRLL
Sbjct: 241 AIFLLGRPVYRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHE-VQSGDKFQGRLL 300

Query: 301 SHTKNLKFLDKAAIIEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVA 360
           SHTKNL+FLDKAAI+EERGNSKEKQGAWRLATVTRVEELKL+LNMIPIWITSLPF ICVA
Sbjct: 301 SHTKNLRFLDKAAIVEERGNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVA 360

Query: 361 QASTFFVKQCGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERG 420
           QASTFF+KQCGTLDR+I N FVIP SSM+CLAAAGMI+SVAIYDKLLVPLLRKTTGNERG
Sbjct: 361 QASTFFIKQCGTLDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTTGNERG 420

Query: 421 ISILQRIGIGMVFSFTTMVVAALVERKRLGQTTHPMSVFWLAPQFFIIGIGDGFALVGLQ 480
           ISILQRIGIGMVFSFTTMVV+ALVERKRL  TTHPMSVFWLAPQFFIIGIGDGFALVGLQ
Sbjct: 421 ISILQRIGIGMVFSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFALVGLQ 480

Query: 481 EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLD 540
           EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSF+ITIVD ITKKSS KSWFGDDLNSSRLD
Sbjct: 481 EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLNSSRLD 540

Query: 541 NFYWLIAGIVAVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANSTV 589
           NFYWLIAGIVAVDLC YVFLA RYTYKSVQKT V DCYD KGPNA+S V
Sbjct: 541 NFYWLIAGIVAVDLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 588

BLAST of PI0016610.1 vs. ExPASy TrEMBL
Match: A0A5A7TPU6 (Protein NRT1/ PTR FAMILY 5.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004790 PE=3 SV=1)

HSP 1 Score: 1073.9 bits (2776), Expect = 2.2e-310
Identity = 540/580 (93.10%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 10  IKRDQL-EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV 69
           +KRDQL EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV 60

Query: 70  IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLL 129
           IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL+ST+IYLLGLSLL
Sbjct: 61  IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTVIYLLGLSLL 120

Query: 130 TMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPE 189
           TMSTLVPSLKPCD ETC+EPRKVHEILF+TAIYLISIGTGGHKPSLESFGADQFDD HPE
Sbjct: 121 TMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPE 180

Query: 190 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV 249
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV 240

Query: 250 YRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFL 309
           YR+RAP GSPLTPLLQVI+AAFRKRKL YP HSSQL+E VQS DKFQGRLLSHTKNL+FL
Sbjct: 241 YRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHE-VQSGDKFQGRLLSHTKNLRFL 300

Query: 310 DKAAIIEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQ 369
           DKAAI+EERGNSKEKQGAWRLATVTRVEELKL+LNMIPIWITSLPF ICVAQASTFF+KQ
Sbjct: 301 DKAAIVEERGNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVAQASTFFIKQ 360

Query: 370 CGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGI 429
           CGTLDR+I N FVIP SSM+CLAAAGMI+SVAIYDKLLVPLLRKTTGNERGISILQRIGI
Sbjct: 361 CGTLDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTTGNERGISILQRIGI 420

Query: 430 GMVFSFTTMVVAALVERKRLGQTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPD 489
           GMVFSFTTMVV+ALVERKRL  TTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPD
Sbjct: 421 GMVFSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPD 480

Query: 490 SMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYWLIAGI 549
           SMRSLGIAFYLSVNGAANFLSSF+ITIVD ITKKSS KSWFGDDLNSSRLDNFYWLIAGI
Sbjct: 481 SMRSLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLNSSRLDNFYWLIAGI 540

Query: 550 VAVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANSTV 589
           VAVDLC YVFLA RYTYKSVQKT V DCYD KGPNA+S V
Sbjct: 541 VAVDLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 579

BLAST of PI0016610.1 vs. ExPASy TrEMBL
Match: A0A6J1CW84 (protein NRT1/ PTR FAMILY 5.6-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014970 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 3.3e-272
Identity = 481/578 (83.22%), Postives = 523/578 (90.48%), Query Frame = 0

Query: 23  VYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIHEDLKTA 82
           V+DSSVDHKG +PLRASTGVWK+SLFIIAIEFSERLSYFGIATSLVIYLT+VI +DLKTA
Sbjct: 30  VHDSSVDHKGRIPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTKVIRQDLKTA 89

Query: 83  ARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPSLKPCDS 142
           ARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL STI+YLLGLSLLT+STLVPSLK C +
Sbjct: 90  ARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLASTIVYLLGLSLLTVSTLVPSLKACGA 149

Query: 143 E-TCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMSFFNWWN 202
           E TC++PRK+HE+LF+TAIY IS+GTGGHKPSLESFGADQFDD H EERKQKMSFFNWWN
Sbjct: 150 EITCDQPRKIHEVLFFTAIYFISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFFNWWN 209

Query: 203 SGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPLGSPLTP 262
           SGLCAGVI GVTLIVYVQ+HVGWG+ G ILTSVMAISLAIFLLGRPVYRFRAPLGSPLTP
Sbjct: 210 SGLCAGVILGVTLIVYVQDHVGWGVSGAILTSVMAISLAIFLLGRPVYRFRAPLGSPLTP 269

Query: 263 LLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFLDKAAIIEERGNSK 322
           LLQV+VAAFR R L YP H S LYEL QS+   QGRLLSHTK LKFLDKAAIIEE GNS 
Sbjct: 270 LLQVLVAAFRNRNLPYPPHPSHLYEL-QSTHNIQGRLLSHTKKLKFLDKAAIIEETGNSS 329

Query: 323 EKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLDREIRNNFV 382
            KQ AWRLATVTRVEELKLILNMIPIWITSLPFGICVAQ STFF+KQC TLDR+I N+F+
Sbjct: 330 GKQSAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQTSTFFIKQCLTLDRKIGNSFI 389

Query: 383 IPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFSFTTMVVAA 442
           +PASSMFCLAAAGMI+SVAIYDK++VP+LRKTTGNERGISILQRIGIGMVFS T+M VAA
Sbjct: 390 MPASSMFCLAAAGMIVSVAIYDKVIVPILRKTTGNERGISILQRIGIGMVFSLTSMAVAA 449

Query: 443 LVERKRLG-------QTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMRSLG 502
           +VERKRL        + +  MSVFWLAPQF IIGI DGFALVGLQEYFYDQVPDSMRS+G
Sbjct: 450 VVERKRLDVVKDNPTKGSMEMSVFWLAPQFLIIGIADGFALVGLQEYFYDQVPDSMRSIG 509

Query: 503 IAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYWLIAGIVAVDLC 562
           IA YLSVNGAANF+SS LIT+VD ITKKS+DKSWFGDDLNSSRLDNFYWLIA +VAV+LC
Sbjct: 510 IALYLSVNGAANFVSSLLITVVDRITKKSNDKSWFGDDLNSSRLDNFYWLIAAVVAVNLC 569

Query: 563 FYVFLARRYTYKSVQKTTVADCYDG----KGPNANSTV 589
            YVFLARRY+YK++QKTTVADCYDG    KG +A+STV
Sbjct: 570 VYVFLARRYSYKTLQKTTVADCYDGDGNDKGRDASSTV 606

BLAST of PI0016610.1 vs. ExPASy TrEMBL
Match: A0A6J1FT20 (protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447038 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 6.6e-265
Identity = 466/572 (81.47%), Postives = 508/572 (88.81%), Query Frame = 0

Query: 18  DDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIHE 77
           DDQI V+DSSVDHK  LPLRASTGVWK+SLFIIAIEFSERLSYFGIATSLVIYLTRVI +
Sbjct: 10  DDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQ 69

Query: 78  DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPSL 137
           DLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL+STI+YL GLSLLT+STLVPSL
Sbjct: 70  DLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLVSTIVYLFGLSLLTLSTLVPSL 129

Query: 138 KPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMSFF 197
           K C SE C+EPRK+HE+LF+TAIYLIS+GTGGHKPSLESFGADQFDD H EERKQKMSFF
Sbjct: 130 KACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFF 189

Query: 198 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPLGS 257
           NWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSVMA SLAIFL+GRPVYR+RAPLGS
Sbjct: 190 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGS 249

Query: 258 PLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFLDKAAIIEER 317
           PLTPLLQV+VAAF +R L YPS+S+ LYE VQ++DK  GRLL+HT  LKFLDKAAI+ E 
Sbjct: 250 PLTPLLQVLVAAFTRRNLPYPSNSAHLYE-VQATDKVHGRLLTHTNRLKFLDKAAIVGET 309

Query: 318 GNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLDREIR 377
            NS  KQ  WRLATVTRVEELKL+LNMIPIWI S+PFGICVAQ  TFF+KQC TLDR+I 
Sbjct: 310 SNSAGKQSPWRLATVTRVEELKLVLNMIPIWIASIPFGICVAQTYTFFIKQCATLDRKIG 369

Query: 378 NNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFSFTTM 437
             F+IPASSMFCLAA GMI+SVAIYD+LLVP+LRKTTGNERGI+ILQRIGIGM+FSF  M
Sbjct: 370 KTFIIPASSMFCLAAVGMIISVAIYDRLLVPILRKTTGNERGITILQRIGIGMIFSFMAM 429

Query: 438 VVAALVERKRLG-------QTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSM 497
            VA LVERKRLG       + +  MSVFWL PQF IIGI DGFALVGLQEYFYDQVPDSM
Sbjct: 430 SVAGLVERKRLGVVKGSPTKGSGAMSVFWLGPQFLIIGIADGFALVGLQEYFYDQVPDSM 489

Query: 498 RSLGIAFYLSVNGAANFLSSFLITIVDWITKKSS-DKSWFGDDLNSSRLDNFYWLIAGIV 557
           RSLGIAFYLSVNGAANF+SS LIT VD IT KSS  KSWFGD+LN+SRLD+FYWLIAGIV
Sbjct: 490 RSLGIAFYLSVNGAANFVSSLLITAVDRITGKSSGGKSWFGDNLNTSRLDHFYWLIAGIV 549

Query: 558 AVDLCFYVFLARRYTYKSVQKTTVADCYDGKG 582
           AV+LCF+V  AR+Y YKSVQ+TTVADC+DG G
Sbjct: 550 AVNLCFFVLFARQYAYKSVQRTTVADCFDGDG 580

BLAST of PI0016610.1 vs. ExPASy TrEMBL
Match: A0A6J1I1F1 (protein NRT1/ PTR FAMILY 5.6-like OS=Cucurbita maxima OX=3661 GN=LOC111470020 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 7.3e-264
Identity = 466/578 (80.62%), Postives = 508/578 (87.89%), Query Frame = 0

Query: 18  DDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIHE 77
           DDQI V+DSSVDHK  LPLRASTGVWK+SLFIIAIEFSERLSYFGIATSLVIYLTRVI +
Sbjct: 10  DDQILVHDSSVDHKRRLPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTRVIRQ 69

Query: 78  DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPSL 137
           DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL ST++YL GLSLLT+STLVPSL
Sbjct: 70  DLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLASTVVYLFGLSLLTLSTLVPSL 129

Query: 138 KPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMSFF 197
           K C SE C+EPRK+HE+LF+TAIYLIS+GTGGHKPSLESFGADQFDD H EERKQKMSFF
Sbjct: 130 KACGSEPCDEPRKLHEVLFFTAIYLISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFF 189

Query: 198 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPLGS 257
           NWWNSGLCAGVIFGVTLIVYVQEHVGWGM GVILTSVMA SLAIFL+GRPVYR+RAPLGS
Sbjct: 190 NWWNSGLCAGVIFGVTLIVYVQEHVGWGMAGVILTSVMATSLAIFLVGRPVYRYRAPLGS 249

Query: 258 PLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFLDKAAIIEER 317
           PLTPLLQV+VAAF +R L YPS+S+ LYE VQ++DK  GRLL+HT  L+FLDKAAI  E 
Sbjct: 250 PLTPLLQVLVAAFTRRNLPYPSNSAHLYE-VQATDKVHGRLLTHTNRLRFLDKAAIAGET 309

Query: 318 GNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLDREIR 377
            NS  KQ  WRLATVTRVEELKL+LNMIPIWI SLPFGICVAQ  TFF+KQC TLDR+I 
Sbjct: 310 SNSAGKQSPWRLATVTRVEELKLVLNMIPIWIASLPFGICVAQTYTFFIKQCATLDRKIG 369

Query: 378 NNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFSFTTM 437
           ++F+IPASSMFCLAA GMI+SVAIYD+LLVP+LRKTT NERGI+ILQRIGIGM+FSF  M
Sbjct: 370 DSFIIPASSMFCLAAVGMIISVAIYDRLLVPILRKTTDNERGITILQRIGIGMIFSFMAM 429

Query: 438 VVAALVERKRLG-------QTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSM 497
            VA LVERKRLG       + +  MSVFWL PQF IIGI DGFALVGLQEYFYDQVPDSM
Sbjct: 430 SVAGLVERKRLGVVKGSPTKGSGAMSVFWLGPQFLIIGIADGFALVGLQEYFYDQVPDSM 489

Query: 498 RSLGIAFYLSVNGAANFLSSFLITIVDWITKKSS-DKSWFGDDLNSSRLDNFYWLIAGIV 557
           RSLGIAFYLSVNG ANF+SS LIT VD IT KSS  KSWFGD+LNSSRLD+FYWLIAGIV
Sbjct: 490 RSLGIAFYLSVNGVANFVSSLLITAVDRITGKSSGGKSWFGDNLNSSRLDHFYWLIAGIV 549

Query: 558 AVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANST 588
           AV+LCF+V  AR Y YKSVQ+TTVADC+DG G    +T
Sbjct: 550 AVNLCFFVLFARHYAYKSVQRTTVADCFDGDGKAGGAT 586

BLAST of PI0016610.1 vs. NCBI nr
Match: XP_008442499.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.6 [Cucumis melo])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 548/589 (93.04%), Postives = 567/589 (96.26%), Query Frame = 0

Query: 1   MKTNNKLEKIKRDQL-EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS 60
           MKTNN+LEK+KRDQL EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS
Sbjct: 1   MKTNNQLEKMKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS 60

Query: 61  YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTI 120
           YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL+ST+
Sbjct: 61  YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTV 120

Query: 121 IYLLGLSLLTMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGA 180
           IYLLGLSLLTMSTLVPSLKPCD ETC+EPRKVHEILF+TAIYLISIGTGGHKPSLESFGA
Sbjct: 121 IYLLGLSLLTMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGA 180

Query: 181 DQFDDHHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL 240
           DQFDD HPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL
Sbjct: 181 DQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL 240

Query: 241 AIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLL 300
           AIFLLGRPVYR+RAP GSPLTPLLQVI+AAFRKRKL YP HSSQL+E VQS DKFQGRLL
Sbjct: 241 AIFLLGRPVYRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHE-VQSGDKFQGRLL 300

Query: 301 SHTKNLKFLDKAAIIEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVA 360
           SHTKNL+FLDKAAI+EERGNSKEKQGAWRLATVTRVEELKL+LNMIPIWITSLPF ICVA
Sbjct: 301 SHTKNLRFLDKAAIVEERGNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVA 360

Query: 361 QASTFFVKQCGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERG 420
           QASTFF+KQCGTLDR+I N FVIP SSM+CLAAAGMI+SVAIYDKLLVPLLRKTTGNERG
Sbjct: 361 QASTFFIKQCGTLDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTTGNERG 420

Query: 421 ISILQRIGIGMVFSFTTMVVAALVERKRLGQTTHPMSVFWLAPQFFIIGIGDGFALVGLQ 480
           ISILQRIGIGMVFSFTTMVV+ALVERKRL  TTHPMSVFWLAPQFFIIGIGDGFALVGLQ
Sbjct: 421 ISILQRIGIGMVFSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFALVGLQ 480

Query: 481 EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLD 540
           EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSF+ITIVD ITKKSS KSWFGDDLNSSRLD
Sbjct: 481 EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLNSSRLD 540

Query: 541 NFYWLIAGIVAVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANSTV 589
           NFYWLIAGIVAVDLC YVFLA RYTYKSVQKT V DCYD KGPNA+S V
Sbjct: 541 NFYWLIAGIVAVDLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 588

BLAST of PI0016610.1 vs. NCBI nr
Match: XP_031737503.1 (protein NRT1/ PTR FAMILY 5.6 [Cucumis sativus] >KAE8650995.1 hypothetical protein Csa_001365 [Cucumis sativus])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 545/589 (92.53%), Postives = 566/589 (96.10%), Query Frame = 0

Query: 1   MKTNNKLEKIKRDQL-EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS 60
           MKTNN+LEKIKRDQL EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS
Sbjct: 1   MKTNNQLEKIKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLS 60

Query: 61  YFGIATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTI 120
           YFGIATSLVIYLT+VIHEDLKTAARNVNYWTGVTTLMPL GGFLADAYLGRFSTVL+ST+
Sbjct: 61  YFGIATSLVIYLTKVIHEDLKTAARNVNYWTGVTTLMPLFGGFLADAYLGRFSTVLISTV 120

Query: 121 IYLLGLSLLTMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGA 180
           IYLLGLSLLT+STLVPSLKPC  ETCEEPRKVHEILF+TAIYLISIGTGGHKPSLESFGA
Sbjct: 121 IYLLGLSLLTLSTLVPSLKPCGGETCEEPRKVHEILFFTAIYLISIGTGGHKPSLESFGA 180

Query: 181 DQFDDHHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL 240
           DQFDD HPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL
Sbjct: 181 DQFDDDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISL 240

Query: 241 AIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLL 300
           AIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFR RKLGYP +SSQL+E VQS DKFQGRLL
Sbjct: 241 AIFLLGRPVYRFRAPLGSPLTPLLQVIVAAFRNRKLGYPPNSSQLHE-VQSGDKFQGRLL 300

Query: 301 SHTKNLKFLDKAAIIEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVA 360
           SHTKNLKFLDKAAI++ER NS+EKQGAWRL+TVTRVEELKL+LNMIPIWITSLPFGICVA
Sbjct: 301 SHTKNLKFLDKAAIVDERDNSEEKQGAWRLSTVTRVEELKLVLNMIPIWITSLPFGICVA 360

Query: 361 QASTFFVKQCGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERG 420
           QASTFFVKQCGTLDR+I NNFVIPASSMFCL+A GMI+SVAIYDKLLVPLLRK TGNERG
Sbjct: 361 QASTFFVKQCGTLDRKIGNNFVIPASSMFCLSAIGMIISVAIYDKLLVPLLRKITGNERG 420

Query: 421 ISILQRIGIGMVFSFTTMVVAALVERKRLGQTTHPMSVFWLAPQFFIIGIGDGFALVGLQ 480
           ISILQRIGIGMVFSFTTMVV+ALVERKRL  TTHPM+VFWLAPQFFIIGIGDGFALVGLQ
Sbjct: 421 ISILQRIGIGMVFSFTTMVVSALVERKRLDHTTHPMNVFWLAPQFFIIGIGDGFALVGLQ 480

Query: 481 EYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLD 540
           EYFYDQVPDSMRSLGIAFYLSVNGAANF SSFLITIVD ITKKSS KSWFGDDLNSSRLD
Sbjct: 481 EYFYDQVPDSMRSLGIAFYLSVNGAANFFSSFLITIVDQITKKSSGKSWFGDDLNSSRLD 540

Query: 541 NFYWLIAGIVAVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANSTV 589
           NFYWLIAGIVAVDLC YVFLARRYTYKSVQKTTVADC+D KG + +S V
Sbjct: 541 NFYWLIAGIVAVDLCVYVFLARRYTYKSVQKTTVADCHDVKGRDVSSAV 588

BLAST of PI0016610.1 vs. NCBI nr
Match: KAA0044126.1 (protein NRT1/ PTR FAMILY 5.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1073.9 bits (2776), Expect = 4.5e-310
Identity = 540/580 (93.10%), Postives = 558/580 (96.21%), Query Frame = 0

Query: 10  IKRDQL-EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV 69
           +KRDQL EEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV
Sbjct: 1   MKRDQLSEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLV 60

Query: 70  IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLL 129
           IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL+ST+IYLLGLSLL
Sbjct: 61  IYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLISTVIYLLGLSLL 120

Query: 130 TMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPE 189
           TMSTLVPSLKPCD ETC+EPRKVHEILF+TAIYLISIGTGGHKPSLESFGADQFDD HPE
Sbjct: 121 TMSTLVPSLKPCDGETCQEPRKVHEILFFTAIYLISIGTGGHKPSLESFGADQFDDDHPE 180

Query: 190 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV 249
           ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV
Sbjct: 181 ERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPV 240

Query: 250 YRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFL 309
           YR+RAP GSPLTPLLQVI+AAFRKRKL YP HSSQL+E VQS DKFQGRLLSHTKNL+FL
Sbjct: 241 YRYRAPSGSPLTPLLQVIIAAFRKRKLDYPPHSSQLHE-VQSGDKFQGRLLSHTKNLRFL 300

Query: 310 DKAAIIEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQ 369
           DKAAI+EERGNSKEKQGAWRLATVTRVEELKL+LNMIPIWITSLPF ICVAQASTFF+KQ
Sbjct: 301 DKAAIVEERGNSKEKQGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVAQASTFFIKQ 360

Query: 370 CGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGI 429
           CGTLDR+I N FVIP SSM+CLAAAGMI+SVAIYDKLLVPLLRKTTGNERGISILQRIGI
Sbjct: 361 CGTLDRKIGNTFVIPVSSMYCLAAAGMIISVAIYDKLLVPLLRKTTGNERGISILQRIGI 420

Query: 430 GMVFSFTTMVVAALVERKRLGQTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPD 489
           GMVFSFTTMVV+ALVERKRL  TTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPD
Sbjct: 421 GMVFSFTTMVVSALVERKRLSHTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPD 480

Query: 490 SMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYWLIAGI 549
           SMRSLGIAFYLSVNGAANFLSSF+ITIVD ITKKSS KSWFGDDLNSSRLDNFYWLIAGI
Sbjct: 481 SMRSLGIAFYLSVNGAANFLSSFIITIVDRITKKSSGKSWFGDDLNSSRLDNFYWLIAGI 540

Query: 550 VAVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANSTV 589
           VAVDLC YVFLA RYTYKSVQKT V DCYD KGPNA+S V
Sbjct: 541 VAVDLCVYVFLAHRYTYKSVQKTAVGDCYDDKGPNADSAV 579

BLAST of PI0016610.1 vs. NCBI nr
Match: XP_038903919.1 (protein NRT1/ PTR FAMILY 5.6-like [Benincasa hispida])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.3e-295
Identity = 523/585 (89.40%), Postives = 547/585 (93.50%), Query Frame = 0

Query: 5   NKLEKIKRD-QLEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 64
           NK +  + D +L +D+Q WVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI
Sbjct: 9   NKRKLFEDDPKLNKDNQTWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 68

Query: 65  ATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLL 124
           ATSLVIYLTRV+HEDLKTAARNVNYWTGVTTLMPLLGGFLADAY GRFSTVL+STIIYLL
Sbjct: 69  ATSLVIYLTRVLHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYFGRFSTVLISTIIYLL 128

Query: 125 GLSLLTMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFD 184
           GLSLLTMSTLVPSLKPC SETCEEPRK+HEILF+TAIYLISIGTGGHKPSLESFGADQFD
Sbjct: 129 GLSLLTMSTLVPSLKPCGSETCEEPRKLHEILFFTAIYLISIGTGGHKPSLESFGADQFD 188

Query: 185 DHHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFL 244
           D HPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQ+ VGWGM GVIL+SVMAISLAIFL
Sbjct: 189 DDHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQDRVGWGMAGVILSSVMAISLAIFL 248

Query: 245 LGRPVYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTK 304
           LGRPVYRFRAPLGSPLTPLLQVIVAAFRKR L YP HSS LYE VQSSDKFQGRLL HTK
Sbjct: 249 LGRPVYRFRAPLGSPLTPLLQVIVAAFRKRNLDYPPHSSHLYE-VQSSDKFQGRLLFHTK 308

Query: 305 NLKFLDKAAIIEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQAST 364
           NL FLDKAAIIE+  NS+ K+GAWRLATVTRVEELKL+LNMIPIWITSLPF ICVAQAST
Sbjct: 309 NLTFLDKAAIIEDTDNSEGKEGAWRLATVTRVEELKLVLNMIPIWITSLPFSICVAQAST 368

Query: 365 FFVKQCGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISIL 424
           FFVKQCGTLDR+I N F+IPASSM+C AAAGMILSVAIYDKLLVPLLRKTTGNERGISIL
Sbjct: 369 FFVKQCGTLDRKIGNKFIIPASSMYCSAAAGMILSVAIYDKLLVPLLRKTTGNERGISIL 428

Query: 425 QRIGIGMVFSFTTMVVAALVERKRLGQTTH--PMSVFWLAPQFFIIGIGDGFALVGLQEY 484
           QRIGIGM+FSFTTMVVAALVERKRL +T     MSVFWLAPQFFIIGIGDGFALVGLQEY
Sbjct: 429 QRIGIGMIFSFTTMVVAALVERKRLIRTAEAMSMSVFWLAPQFFIIGIGDGFALVGLQEY 488

Query: 485 FYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNF 544
           FYDQVPDSMRSLGIAFYLS+NGAANFLSS LITI D ITKKSS KSWFG+DLNSSRLDNF
Sbjct: 489 FYDQVPDSMRSLGIAFYLSINGAANFLSSLLITIADRITKKSSGKSWFGEDLNSSRLDNF 548

Query: 545 YWLIAGIVAVDLCFYVFLARRYTYKSVQKTTVADCYDGKGPNANS 587
           YWLIA IVAVDLC YVFLARRYTYKSVQKTTVADCYDGKGP+A+S
Sbjct: 549 YWLIAAIVAVDLCVYVFLARRYTYKSVQKTTVADCYDGKGPDASS 592

BLAST of PI0016610.1 vs. NCBI nr
Match: XP_022145543.1 (protein NRT1/ PTR FAMILY 5.6-like isoform X1 [Momordica charantia])

HSP 1 Score: 947.2 bits (2447), Expect = 6.7e-272
Identity = 481/578 (83.22%), Postives = 523/578 (90.48%), Query Frame = 0

Query: 23  VYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIHEDLKTA 82
           V+DSSVDHKG +PLRASTGVWK+SLFIIAIEFSERLSYFGIATSLVIYLT+VI +DLKTA
Sbjct: 30  VHDSSVDHKGRIPLRASTGVWKASLFIIAIEFSERLSYFGIATSLVIYLTKVIRQDLKTA 89

Query: 83  ARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPSLKPCDS 142
           ARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVL STI+YLLGLSLLT+STLVPSLK C +
Sbjct: 90  ARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLASTIVYLLGLSLLTVSTLVPSLKACGA 149

Query: 143 E-TCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMSFFNWWN 202
           E TC++PRK+HE+LF+TAIY IS+GTGGHKPSLESFGADQFDD H EERKQKMSFFNWWN
Sbjct: 150 EITCDQPRKIHEVLFFTAIYFISVGTGGHKPSLESFGADQFDDDHAEERKQKMSFFNWWN 209

Query: 203 SGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPLGSPLTP 262
           SGLCAGVI GVTLIVYVQ+HVGWG+ G ILTSVMAISLAIFLLGRPVYRFRAPLGSPLTP
Sbjct: 210 SGLCAGVILGVTLIVYVQDHVGWGVSGAILTSVMAISLAIFLLGRPVYRFRAPLGSPLTP 269

Query: 263 LLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFLDKAAIIEERGNSK 322
           LLQV+VAAFR R L YP H S LYEL QS+   QGRLLSHTK LKFLDKAAIIEE GNS 
Sbjct: 270 LLQVLVAAFRNRNLPYPPHPSHLYEL-QSTHNIQGRLLSHTKKLKFLDKAAIIEETGNSS 329

Query: 323 EKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLDREIRNNFV 382
            KQ AWRLATVTRVEELKLILNMIPIWITSLPFGICVAQ STFF+KQC TLDR+I N+F+
Sbjct: 330 GKQSAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQTSTFFIKQCLTLDRKIGNSFI 389

Query: 383 IPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFSFTTMVVAA 442
           +PASSMFCLAAAGMI+SVAIYDK++VP+LRKTTGNERGISILQRIGIGMVFS T+M VAA
Sbjct: 390 MPASSMFCLAAAGMIVSVAIYDKVIVPILRKTTGNERGISILQRIGIGMVFSLTSMAVAA 449

Query: 443 LVERKRLG-------QTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMRSLG 502
           +VERKRL        + +  MSVFWLAPQF IIGI DGFALVGLQEYFYDQVPDSMRS+G
Sbjct: 450 VVERKRLDVVKDNPTKGSMEMSVFWLAPQFLIIGIADGFALVGLQEYFYDQVPDSMRSIG 509

Query: 503 IAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYWLIAGIVAVDLC 562
           IA YLSVNGAANF+SS LIT+VD ITKKS+DKSWFGDDLNSSRLDNFYWLIA +VAV+LC
Sbjct: 510 IALYLSVNGAANFVSSLLITVVDRITKKSNDKSWFGDDLNSSRLDNFYWLIAAVVAVNLC 569

Query: 563 FYVFLARRYTYKSVQKTTVADCYDG----KGPNANSTV 589
            YVFLARRY+YK++QKTTVADCYDG    KG +A+STV
Sbjct: 570 VYVFLARRYSYKTLQKTTVADCYDGDGNDKGRDASSTV 606

BLAST of PI0016610.1 vs. TAIR 10
Match: AT2G37900.1 (Major facilitator superfamily protein )

HSP 1 Score: 740.3 bits (1910), Expect = 1.2e-213
Identity = 362/556 (65.11%), Postives = 444/556 (79.86%), Query Frame = 0

Query: 17  EDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIH 76
           +D Q WV DSS+D +G +PLRA TG W+++LFIIAIEFSERLSYFG+AT+LV+YLT +++
Sbjct: 16  DDQQKWVLDSSLDSRGRVPLRARTGAWRAALFIIAIEFSERLSYFGLATNLVVYLTTILN 75

Query: 77  EDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPS 136
           +DLK A RNVNYW+GVTTLMPLLGGF+ADAYLGR++TVL++T IYL+GL LLTMS  +P 
Sbjct: 76  QDLKMAIRNVNYWSGVTTLMPLLGGFIADAYLGRYATVLVATTIYLMGLVLLTMSWFIPG 135

Query: 137 LKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMSF 196
           LKPC  E C EPRK HE+ F+ AIYLISIGTGGHKPSLESFGADQFDD H EERK KMSF
Sbjct: 136 LKPCHQEVCVEPRKAHEVAFFIAIYLISIGTGGHKPSLESFGADQFDDDHVEERKMKMSF 195

Query: 197 FNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPLG 256
           FNWWN  LCAG++  VT + Y+++ VGWG+ G+ILT VMAISL IF +G+P YR+R P G
Sbjct: 196 FNWWNVSLCAGILTAVTAVAYIEDRVGWGVAGIILTVVMAISLIIFFIGKPFYRYRTPSG 255

Query: 257 SPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTKNLKFLDKAAIIEE 316
           SPLTP+LQV VAA  KR L YPS  S L+E V  ++   GRLL HT++LKFLDKAAIIE+
Sbjct: 256 SPLTPILQVFVAAIAKRNLPYPSDPSLLHE-VSKTEFTSGRLLCHTEHLKFLDKAAIIED 315

Query: 317 RGN-SKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLDRE 376
           +   + EKQ  WRL T+T+VEE KLI+N+IPIW ++L FGIC  QASTFF+KQ  T+DR 
Sbjct: 316 KNPLALEKQSPWRLLTLTKVEETKLIINVIPIWFSTLAFGICATQASTFFIKQAITMDRH 375

Query: 377 IRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFSFT 436
           I   F +P +SMF L A  +I+S+ +Y+KLLVPLLR  T N+RGI+ILQRIG GM+FS  
Sbjct: 376 I-GGFTVPPASMFTLTALTLIISLTVYEKLLVPLLRSITRNQRGINILQRIGTGMIFSLI 435

Query: 437 TMVVAALVERKRLGQTTH--PMSVFWLAPQFFIIGIGDGFALVGLQEYFYDQVPDSMRSL 496
           TM++AALVE++RL +T +  PMSV WLAPQF +IG  D F LVGLQEYFY QVPDSMRSL
Sbjct: 436 TMIIAALVEKQRLDRTNNNKPMSVIWLAPQFMVIGFADAFTLVGLQEYFYHQVPDSMRSL 495

Query: 497 GIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYWLIAGIVAVDL 556
           GIAFYLSV GAA+FL++ LIT VD + +  S KSWFG DLNSSRLD FYW +AG++A ++
Sbjct: 496 GIAFYLSVIGAASFLNNLLITAVDTLAENFSGKSWFGKDLNSSRLDRFYWFLAGVIAANI 555

Query: 557 CFYVFLARRYTYKSVQ 570
           C +V +A+R  YKSVQ
Sbjct: 556 CVFVIVAKRCPYKSVQ 569

BLAST of PI0016610.1 vs. TAIR 10
Match: AT3G53960.1 (Major facilitator superfamily protein )

HSP 1 Score: 733.0 bits (1891), Expect = 1.9e-211
Identity = 362/584 (61.99%), Postives = 457/584 (78.25%), Query Frame = 0

Query: 4   NNKLEKIKRDQLEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGI 63
           +NK++   +D   +D Q WV DSS D +G +PLRA TG W+++LFII IEFSERLSYFGI
Sbjct: 3   HNKVDTEPQDSY-DDQQKWVLDSSTDSRGEIPLRAQTGAWRAALFIIGIEFSERLSYFGI 62

Query: 64  ATSLVIYLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLL 123
           +T+LV+YLT ++H+DLK A +N NYW+GVTTLMPLLGGF+ADAYLGR+ TVLL+T IYL+
Sbjct: 63  STNLVVYLTTILHQDLKMAVKNTNYWSGVTTLMPLLGGFVADAYLGRYGTVLLATTIYLM 122

Query: 124 GLSLLTMSTLVPSLKPCDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFD 183
           GL LLT+S  +P LK C  + C EPRK HEI F+ AIYLISIGTGGHKPSLESFGADQF+
Sbjct: 123 GLILLTLSWFIPGLKACHEDMCVEPRKAHEIAFFIAIYLISIGTGGHKPSLESFGADQFE 182

Query: 184 DHHPEERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFL 243
           D HPEERK KMS+FNWWN+GLCAG++  VT+IVY+++ +GWG+  +ILT VMA S  IF 
Sbjct: 183 DGHPEERKMKMSYFNWWNAGLCAGILTAVTVIVYIEDRIGWGVASIILTIVMATSFFIFR 242

Query: 244 LGRPVYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGRLLSHTK 303
           +G+P YR+RAP GSPLTP+LQV VAA  KR L  PS SS L+EL  + +  +GRLLS +K
Sbjct: 243 IGKPFYRYRAPSGSPLTPMLQVFVAAIAKRNLPCPSDSSLLHELT-NEEYTKGRLLSSSK 302

Query: 304 NLKFLDKAAIIEERG-NSK-EKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQA 363
           NLKFLDKAA+IE+R  N+K EKQ  WRLATVT+VEE+KL++NMIPIW  +L FG+C  Q+
Sbjct: 303 NLKFLDKAAVIEDRNENTKAEKQSPWRLATVTKVEEVKLLINMIPIWFFTLAFGVCATQS 362

Query: 364 STFFVKQCGTLDREIR-NNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGI 423
           ST F+KQ   +DR I   +F++P +S+F L A  +I++V IY+KLLVPLLR+ TGNERGI
Sbjct: 363 STLFIKQAIIMDRHITGTSFIVPPASLFSLIALSIIITVTIYEKLLVPLLRRATGNERGI 422

Query: 424 SILQRIGIGMVFSFTTMVVAALVERKRLG-------QTTHPMSVFWLAPQFFIIGIGDGF 483
           SILQRIG+GMVFS   M++AAL+E+KRL          T  +S  WLAPQF ++G+ D F
Sbjct: 423 SILQRIGVGMVFSLFAMIIAALIEKKRLDYAKEHHMNKTMTLSAIWLAPQFLVLGVADAF 482

Query: 484 ALVGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDL 543
            LVGLQEYFYDQVPDSMRSLGIAFYLSV GAA+F+++ LIT+ D + ++ S K WFG DL
Sbjct: 483 TLVGLQEYFYDQVPDSMRSLGIAFYLSVLGAASFVNNLLITVSDHLAEEISGKGWFGKDL 542

Query: 544 NSSRLDNFYWLIAGIVAVDLCFYVFLARRYTYKSVQKT--TVAD 576
           NSSRLD FYW++A + A ++C +V +A RYTYK+VQ +   VAD
Sbjct: 543 NSSRLDRFYWMLAALTAANICCFVIVAMRYTYKTVQPSLAVVAD 584

BLAST of PI0016610.1 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 454.5 bits (1168), Expect = 1.3e-127
Identity = 242/568 (42.61%), Postives = 351/568 (61.80%), Query Frame = 0

Query: 17  EDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRVIH 76
           E  +++  D +VD +G   L + TG W++  F++  E  ER++++GIA++LV YLT+ +H
Sbjct: 2   EAAKVYTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLH 61

Query: 77  EDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLVPS 136
           ED  ++ RNVN W+G   + P+ G ++AD+Y+GRF T   S++IY+LG+ LLTM+  V S
Sbjct: 62  EDTISSVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKS 121

Query: 137 LKP-CDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQKMS 196
           L+P C++  C +   +    FY ++Y I+IG GG KP++ +FGADQFD +  EE+KQK+S
Sbjct: 122 LRPTCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVS 181

Query: 197 FFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRAPL 256
           FFNWW      G +F    +VY+QE++GWG+G  I T  + +SL +F +G P YR +   
Sbjct: 182 FFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIK 241

Query: 257 GSPLT-PLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQGR-LLSHTKNLKFLDKAAI 316
              L   L+QV +AAF+ RKL  P    +LYEL     K  G+  + HT   +FLDKAAI
Sbjct: 242 TDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAI 301

Query: 317 IEERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGTLD 376
                    K  +    TVT+VE  K +L +I IW+ +L      AQ +T FVKQ  TLD
Sbjct: 302 ---------KTSSRVPCTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLD 361

Query: 377 REIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMVFS 436
           R+I +NF IPA+S+       M+LSV +YD+  VP +RK TGN RGI++LQR+G+G    
Sbjct: 362 RKIGSNFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQ 421

Query: 437 FTTMVVAALVERKRLGQTTH----------PMSVFWLAPQFFIIGIGDGFALVGLQEYFY 496
              + +A+ VE KR+               PMS+FWL PQ+ ++GIGD F  +GL E+FY
Sbjct: 422 IVAIAIASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFY 481

Query: 497 DQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFYW 556
           DQ P+ M+SLG  F+ S  G  NFL+SFL+T++D IT K   KSW G++LN SRLD +Y 
Sbjct: 482 DQSPEEMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYG 541

Query: 557 LIAGIVAVDLCFYVFLARRYTYKSVQKT 572
            +  I  V++  +V+ A +Y YKS   T
Sbjct: 542 FLVVISIVNMGLFVWAASKYVYKSDDDT 560

BLAST of PI0016610.1 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 449.9 bits (1156), Expect = 3.1e-126
Identity = 238/571 (41.68%), Postives = 348/571 (60.95%), Query Frame = 0

Query: 10  IKRDQLEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVI 69
           I+   + ++ +++  D SVD  GN PL+  TG WK+  FI+  E  ERL+Y+GIA +L+ 
Sbjct: 12  IEEGLILQEVKLYAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLIT 71

Query: 70  YLTRVIHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLT 129
           YLT  +H+   +AA NV  W G   L PL+G  LADAY GR+ T+   + IY +G+S LT
Sbjct: 72  YLTTKLHQGNVSAATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALT 131

Query: 130 MSTLVPSLKP--CDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHP 189
           +S  VP+LKP  C  + C         +F+  +YLI++GTGG KP + SFGADQFDD   
Sbjct: 132 LSASVPALKPAECIGDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDS 191

Query: 190 EERKQKMSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRP 249
            ER +K SFFNW+   +  G +   +L+V++QE+ GWG+G  I T  M +++A F  G P
Sbjct: 192 RERVRKASFFNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTP 251

Query: 250 VYRFRAPLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQG-RLLSHTKNLK 309
           +YRF+ P GSP+T + QV+VA+FRK  +  P  ++ LYE    +    G R + HT + +
Sbjct: 252 LYRFQKPGGSPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQ 311

Query: 310 FLDKAAII-EERGNSKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFF 369
           +LDKAA+I EE   S +   +WRL TVT+VEELK+++ M PIW + + F    AQ ST F
Sbjct: 312 YLDKAAVISEEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMF 371

Query: 370 VKQCGTLDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQR 429
           V+Q   ++ +I  +F +P +++     A +I+ V +YD+ +VPL RK TG ++G + +QR
Sbjct: 372 VQQGRAMNCKI-GSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQR 431

Query: 430 IGIGMVFSFTTMVVAALVERKRLGQTTH----------PMSVFWLAPQFFIIGIGDGFAL 489
           +GIG+  S   M  AA+VE  RL               P+SV W  PQ+FI+G  + F  
Sbjct: 432 MGIGLFVSVLCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYF 491

Query: 490 VGLQEYFYDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNS 549
           +G  E+FYDQ PD+MRSL  A  L  N   N+LSS ++T+V + T ++  + W  D+LNS
Sbjct: 492 IGQLEFFYDQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNS 551

Query: 550 SRLDNFYWLIAGIVAVDLCFYVFLARRYTYK 567
             LD F+WL+AG+  V++  Y F A RY  K
Sbjct: 552 GHLDYFFWLLAGLSLVNMAVYFFSAARYKQK 581

BLAST of PI0016610.1 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 445.3 bits (1144), Expect = 7.7e-125
Identity = 238/570 (41.75%), Postives = 348/570 (61.05%), Query Frame = 0

Query: 15  LEEDDQIWVYDSSVDHKGNLPLRASTGVWKSSLFIIAIEFSERLSYFGIATSLVIYLTRV 74
           +E+D  I+  D ++D       +  TG WK+  FI+  E  ERL+Y+G++T+L+ YL + 
Sbjct: 1   MEDDKDIYTKDGTLDIHKKPANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQ 60

Query: 75  IHEDLKTAARNVNYWTGVTTLMPLLGGFLADAYLGRFSTVLLSTIIYLLGLSLLTMSTLV 134
           ++ +  +A+++V+ W+G     PL+G F+ADAYLGR+ T+    +IY+ G++LLT+S  V
Sbjct: 61  MNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASV 120

Query: 135 PSLKP-CDSETCEEPRKVHEILFYTAIYLISIGTGGHKPSLESFGADQFDDHHPEERKQK 194
           P L P C  ETC        I F  A+YLI++GTGG KP + SFGADQFDD   +E++ K
Sbjct: 121 PGLTPTCSGETCHATAGQTAITF-IALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESK 180

Query: 195 MSFFNWWNSGLCAGVIFGVTLIVYVQEHVGWGMGGVILTSVMAISLAIFLLGRPVYRFRA 254
            SFFNW+   +  G +   +++V++Q +VGWG G  + T  MAI++  F  G   YR + 
Sbjct: 181 SSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQK 240

Query: 255 PLGSPLTPLLQVIVAAFRKRKLGYPSHSSQLYELVQSSDKFQG-RLLSHTKNLKFLDKAA 314
           P GSPLT +LQVIVA+ RK K+  P   S LYE   +     G R L HTK L F DKAA
Sbjct: 241 PGGSPLTRMLQVIVASCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAA 300

Query: 315 IIEERGN-SKEKQGAWRLATVTRVEELKLILNMIPIWITSLPFGICVAQASTFFVKQCGT 374
           +  E  N    K  +W+L TVT+VEELK ++ ++PIW T + F    +Q  T FV Q  T
Sbjct: 301 VETESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNT 360

Query: 375 LDREIRNNFVIPASSMFCLAAAGMILSVAIYDKLLVPLLRKTTGNERGISILQRIGIGMV 434
           LD+ +  NF IP++S+       ++    +YDKL+VP  RK TG+ERG + LQRIGIG+V
Sbjct: 361 LDQHMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLV 420

Query: 435 FSFTTMVVAALVERKRL---------GQTTHPMSVFWLAPQFFIIGIGDGFALVGLQEYF 494
            S  +MV A ++E  RL          + T PM++FW  PQ+F++G  + F  +G  E+F
Sbjct: 421 ISIFSMVSAGILEVARLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFF 480

Query: 495 YDQVPDSMRSLGIAFYLSVNGAANFLSSFLITIVDWITKKSSDKSWFGDDLNSSRLDNFY 554
           YDQ PD+MRSL  A  L+     N+LS+FL+T+V  +T+      W   +LN+  LD F+
Sbjct: 481 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFF 540

Query: 555 WLIAGIVAVDLCFYVFLARRYTYKSVQKTT 573
           WL+AG+  ++   Y+++A+ YTYK   KTT
Sbjct: 541 WLLAGLSFLNFLVYLWIAKWYTYK---KTT 566