PI0008720.1 (mRNA) Melon (PI 482460) v1

BLAST of PI0008720.1 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.5e-138
Identity = 252/542 (46.49%), Postives = 356/542 (65.68%), Query Frame = 0

Query: 2   KSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDI-----PIYTPINSSY 61
           +SF  + L+F + + + L      +A+    + FLQCLS+   D       I+TP ++S+
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 63

Query: 62  SSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGL 121
           SS+L S  QN RFS      KP++I+ P   S +QS V C++  G+ IR  SGGHD EGL
Sbjct: 64  SSVLASSIQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGL 123

Query: 122 SYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTL 181
           SY++  PF+++DL NL+SI VD  N + WVQ+GATIG          ELYY IG+K++TL
Sbjct: 124 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIG----------ELYYEIGKKNRTL 183

Query: 182 AFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWA 241
           AFPAG CPTVGVGGH SGGGYG L+RK+G+AADHVIDA +VDA G++  +  MGED FWA
Sbjct: 184 AFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWA 243

Query: 242 IRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLL 301
           IRGGGG SF +V++WK+ L+ VP+ VT+    +  E+   LK+IH WQ+VA K+  +L +
Sbjct: 244 IRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQS-ALKIIHRWQFVADKVSDDLFI 303

Query: 302 GISLTDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGW 361
            + L   +  +   ASF  ++LG  + +L ++   FP+LGL ++DC EMSWI+SV+   W
Sbjct: 304 RVMLQRYKNMVR--ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---W 363

Query: 362 FQK--GDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVP 421
           F +   +P+ VL  RTR  S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P
Sbjct: 364 FAELGEEPINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTP 423

Query: 422 YGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKS 481
           +GGKMSE+ D+ETPF HR    Y + Y+  W+    + ++ ++ W+  +YD M+ FV+KS
Sbjct: 424 FGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKS 483

Query: 482 PRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQS 537
           PR AY+N RDLD+G      ++ Y+  + WG+KYF NNF+RLV VKT VDPSDFF  EQS
Sbjct: 484 PRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQS 524

BLAST of PI0008720.1 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.3e-138
Identity = 255/538 (47.40%), Postives = 354/538 (65.80%), Query Frame = 0

Query: 20  FSTQWSTASSYKNNDFLQCLSIHSRDIP--------IYTPINSSYSSILRSYAQNPRFST 79
           F+ Q S A+  +N  FL+CLS +   IP        +YT  +  Y SIL S  QN RF T
Sbjct: 21  FNIQISIANPQEN--FLKCLSQY---IPTNVTNAKLVYTQHDQFYMSILNSTVQNLRF-T 80

Query: 80  QHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYISDLPFIVVDLIN 139
              T KPLVI  P +VSHIQ T++CSK  GLQIR  SGGHD EG+SYIS +PF++VDL N
Sbjct: 81  SDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRN 140

Query: 140 LKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPAGSCPTVGVGGH 199
           + S+K+D  + TAWV+SGAT+GE+YY I E +E           L+FPAG CPTVG GGH
Sbjct: 141 MHSVKIDVHSQTAWVESGATLGEVYYWINENNE----------NLSFPAGYCPTVGTGGH 200

Query: 200 LSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 259
            SGGGYG L+R YG+AAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 201 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 260

Query: 260 KVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGI-----SLTDEEGK 319
           K++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL       ++TD +GK
Sbjct: 261 KIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGK 320

Query: 320 INPI--ASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVL----LMGWFQKG 379
                 + FS +F G  + ++ ++  +FP+LG+ K DC ++SWI +++    ++ +    
Sbjct: 321 NKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTN 380

Query: 380 DPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPYGGKMS 439
              E+LL+R+        IK DYVK+ IP  A+  + E+L  +DV +   +F PYGG M 
Sbjct: 381 FKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMD 440

Query: 440 EVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSPRAAYV 499
           E+ +   PF HR+  +Y + YIA W+    E  + H+NWIR +Y++ TP+VS++PR AY+
Sbjct: 441 EISESAIPFPHRAGITYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPRMAYL 500

Query: 500 NYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSIPTL 539
           NYRDLD+G  N     NY +AR+WG KYFG NF+RLV VKTKVDP +FFR+EQSIP L
Sbjct: 501 NYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of PI0008720.1 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.8e-137
Identity = 256/542 (47.23%), Postives = 354/542 (65.31%), Query Frame = 0

Query: 20  FSTQWSTASSYKNNDFLQCLSIHSRDIP--------IYTPINSSYSSILRSYAQNPRFST 79
           F+ Q S A+  +N  FL+C S +   IP        +YT  +  Y SIL S  QN RF T
Sbjct: 21  FNIQISIANPQEN--FLKCFSQY---IPTNVTNAKLVYTQHDQFYMSILNSTIQNLRF-T 80

Query: 80  QHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYISDLPFIVVDLIN 139
              T KPLVII P +VSHIQ T++CSK  GLQIR  SGGHD EG+SYIS +PF++VDL N
Sbjct: 81  SDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRN 140

Query: 140 LKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPAGSCPTVGVGGH 199
           + S+K+D  + TAWV++GAT+GE+YY I E +E           L+FPAG CPTVG GGH
Sbjct: 141 MHSVKIDVHSQTAWVEAGATLGEVYYWINENNE----------NLSFPAGYCPTVGAGGH 200

Query: 200 LSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAW 259
            SGGGYG L+R YG+AAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AW
Sbjct: 201 FSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAW 260

Query: 260 KVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGI-----SLTDEEGK 319
           K++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL       ++TD +GK
Sbjct: 261 KIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGK 320

Query: 320 INPI--ASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMG--------W 379
                 + FS +F G  + ++ ++  +FP+LG+ K DC ++SWI +++           +
Sbjct: 321 NKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTY 380

Query: 380 FQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPYG 439
           F+K    E+LL+R+        IK DYVK+ IP  A+  + E+L  +DV +   +F PYG
Sbjct: 381 FKK----EILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYG 440

Query: 440 GKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSPR 499
           G M E+ +   PF HR+   Y + YIA W+    E  + H+NWIR +Y++ TP+VS++PR
Sbjct: 441 GIMDEISESAIPFPHRAGIMYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVSQNPR 500

Query: 500 AAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSIP 539
            AY+NYRDLD+G  N     NY +AR+WG KYFG NF+RLV VKTKVDP +FFR+EQSIP
Sbjct: 501 MAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIP 540

BLAST of PI0008720.1 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.0e-136
Identity = 254/535 (47.48%), Postives = 349/535 (65.23%), Query Frame = 0

Query: 20  FSTQWSTASSYKNNDFLQCLSIH-----SRDIPIYTPINSSYSSILRSYAQNPRFSTQHK 79
           F  Q S A+  +N  FL+C S H     +    +YT  +  Y SIL S  QN RF +   
Sbjct: 21  FHIQISIANPREN--FLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISD-T 80

Query: 80  TLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYISDLPFIVVDLINLKS 139
           T KPLVI+ PS+ SHIQ+T++CSK  GLQIR  SGGHD EG+SYIS +PF+VVDL N+ S
Sbjct: 81  TPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHS 140

Query: 140 IKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPAGSCPTVGVGGHLSG 199
           IK+D  + TAWV++GAT+GE+YY I EK+E           L+FP G CPTVGVGGH SG
Sbjct: 141 IKIDVHSQTAWVEAGATLGEVYYWINEKNE----------NLSFPGGYCPTVGVGGHFSG 200

Query: 200 GGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVK 259
           GGYG L+R YG+AAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AWK+K
Sbjct: 201 GGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIK 260

Query: 260 LVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGI-----SLTDEEGKINP 319
           LV VP++ TI    + +E   ++KL ++WQ +A K D +L+L       ++TD  GK   
Sbjct: 261 LVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKT 320

Query: 320 IAS--FSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVL----LMGWFQKGDPL 379
                FS +F G  + ++ ++  +FP+LG+ K DC E SWI + +    ++ +       
Sbjct: 321 TVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKK 380

Query: 380 EVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPYGGKMSEVD 439
           E+LL+R+        IK DYVK+ IP  A+  + E+L  +DV     +  PYGG M E+ 
Sbjct: 381 EILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEIS 440

Query: 440 DWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSPRAAYVNYR 499
           +   PF HR+   Y + Y A W+    E  + H+NW+R +Y++ TP+VS++PR AY+NYR
Sbjct: 441 ESAIPFPHRAGIMYELWYTASWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYR 500

Query: 500 DLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSIPTL 539
           DLD+G  N     NY +AR+WG KYFG NF+RLV VKTKVDP++FFR+EQSIP L
Sbjct: 501 DLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of PI0008720.1 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.8e-136
Identity = 251/535 (46.92%), Postives = 351/535 (65.61%), Query Frame = 0

Query: 20  FSTQWSTASSYKNNDFLQCLSIHSRDIP-----IYTPINSSYSSILRSYAQNPRFSTQHK 79
           F+ Q S A+  +N  FL+C S +  + P     IYT  +  Y S+L S  QN RF T   
Sbjct: 21  FNIQISIANPQEN--FLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRF-TSDT 80

Query: 80  TLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYISDLPFIVVDLINLKS 139
           T KPLVI+ PS+VSHIQ++++CSK  GLQIR  SGGHD EGLSYIS +PF +VDL N+ +
Sbjct: 81  TPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHT 140

Query: 140 IKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPAGSCPTVGVGGHLSG 199
           +KVD  + TAWV++GAT+GE+YY I E +E            +FP G CPTVGVGGH SG
Sbjct: 141 VKVDIHSQTAWVEAGATLGEVYYWINEMNE----------NFSFPGGYCPTVGVGGHFSG 200

Query: 200 GGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGSFGIVVAWKVK 259
           GGYG L+R YG+AAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG +FGI+ AWK+K
Sbjct: 201 GGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIK 260

Query: 260 LVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGI-----SLTDEEGKINP 319
           LV VP++ TI    + +E   ++KL ++WQ +A K D +L+L       ++TD  GK   
Sbjct: 261 LVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKT 320

Query: 320 IAS--FSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVL----LMGWFQKGDPL 379
                FS +FLG  + ++ ++  +FP+LG+ K DC E+SWI + +    ++ +       
Sbjct: 321 TVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKK 380

Query: 380 EVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPYGGKMSEVD 439
           E+LL+R+        IK DYVK+ IP  A+  + E+L  ++V +   +  PYGG M E+ 
Sbjct: 381 EILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEIS 440

Query: 440 DWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSPRAAYVNYR 499
           +   PF HR+   Y + Y A W+    E  + H+NW+R +Y++ TP+VS++PR AY+NYR
Sbjct: 441 ESAIPFPHRAGIMYELWYTATWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYR 500

Query: 500 DLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSIPTL 539
           DLD+G  N     NY +AR+WG KYFG NF+RLV VKTK DP++FFR+EQSIP L
Sbjct: 501 DLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of PI0008720.1 vs. ExPASy TrEMBL
Match: A0A5D3C112 (Cannabidiolic acid synthase-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00400 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.5e-258
Identity = 460/564 (81.56%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MKSFPL--NPLLFISFTVVSLFSTQWSTASSYKNNDFLQCL----SIHSRDIP--IYTPI 60
           MK FP+   P   I+F +V + ST  ++  + + + F+QCL    S +S  I   IYTP 
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSYSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHD 120
           NSSYSSIL    QN RF  ++ + KPL II PSHVSHIQ+ VICSKSHG QIR  SGGHD
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNIS-KPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120

Query: 121 TEGLSYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEK 180
            EGLSYIS +PF++VDLINL SI +D  N  AWV+SGAT+GELYYRIA          EK
Sbjct: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIA----------EK 180

Query: 181 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGED 240
           SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVHNKESMGED
Sbjct: 181 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGED 240

Query: 241 LFWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDA 300
           LFWAIRGGGGG FGIVVAWKVKLV VPARVTICEANRTLEE RILKLIHEWQYVAPKLDA
Sbjct: 241 LFWAIRGGGGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDA 300

Query: 301 NLLLGISLT------DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMS 360
           NLLLGISLT      DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQL LTKEDCLEMS
Sbjct: 301 NLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMS 360

Query: 361 WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVE 420
           WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIP+VALKGMWERLKS+DVE
Sbjct: 361 WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVE 420

Query: 421 LSQIMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDY 480
           LSQIMFVPYGGKMSEVDD ETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDY
Sbjct: 421 LSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDY 480

Query: 481 MTPFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPS 540
           MTPFVSKSPRAAYVNYRDLDIGTNNKYG+T+YKRARVWGLKYFG NFD+LV+VKTKVDPS
Sbjct: 481 MTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPS 540

Query: 541 DFFRHEQSIPTLSGISEESFSASL 551
           DFFRH+QSIPTLSGISEESFSASL
Sbjct: 541 DFFRHQQSIPTLSGISEESFSASL 553

BLAST of PI0008720.1 vs. ExPASy TrEMBL
Match: A0A1S3BEA9 (cannabidiolic acid synthase-like 2 OS=Cucumis melo OX=3656 GN=LOC103488685 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.5e-258
Identity = 460/564 (81.56%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MKSFPL--NPLLFISFTVVSLFSTQWSTASSYKNNDFLQCL----SIHSRDIP--IYTPI 60
           MK FP+   P   I+F +V + ST  ++  + + + F+QCL    S +S  I   IYTP 
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSYSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHD 120
           NSSYSSIL    QN RF  ++ + KPL II PSHVSHIQ+ VICSKSHG QIR  SGGHD
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNIS-KPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120

Query: 121 TEGLSYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEK 180
            EGLSYIS +PF++VDLINL SI +D  N  AWV+SGAT+GELYYRIA          EK
Sbjct: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIA----------EK 180

Query: 181 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGED 240
           SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVHNKESMGED
Sbjct: 181 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGED 240

Query: 241 LFWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDA 300
           LFWAIRGGGGG FGIVVAWKVKLV VPARVTICEANRTLEE RILKLIHEWQYVAPKLDA
Sbjct: 241 LFWAIRGGGGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDA 300

Query: 301 NLLLGISLT------DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMS 360
           NLLLGISLT      DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQL LTKEDCLEMS
Sbjct: 301 NLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMS 360

Query: 361 WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVE 420
           WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIP+VALKGMWERLKS+DVE
Sbjct: 361 WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVE 420

Query: 421 LSQIMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDY 480
           LSQIMFVPYGGKMSEVDD ETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDY
Sbjct: 421 LSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDY 480

Query: 481 MTPFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPS 540
           MTPFVSKSPRAAYVNYRDLDIGTNNKYG+T+YKRARVWGLKYFG NFD+LV+VKTKVDPS
Sbjct: 481 MTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPS 540

Query: 541 DFFRHEQSIPTLSGISEESFSASL 551
           DFFRH+QSIPTLSGISEESFSASL
Sbjct: 541 DFFRHQQSIPTLSGISEESFSASL 553

BLAST of PI0008720.1 vs. ExPASy TrEMBL
Match: A0A5A7TYM8 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00390 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.8e-217
Identity = 378/544 (69.49%), Postives = 437/544 (80.33%), Query Frame = 0

Query: 1   MKSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDIPIYTPINSSYSSIL 60
           MKSFPLNPLLF SFTVVSL STQWSTASSYKN DFL+CLSIHS DIPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYIS 120
           RSYAQNPRFSTQ+KTLKPL IIKPSHVSHIQSTVICSKSHGLQIRI SGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPA 180
           DLPFIVVDLINLKSI+VDTTNNT WVQSGATIGELYYRIA          EKS+TLAFPA
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIA----------EKSRTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           G CPTVGVGG  SGGGYGWL+RKYG+AAD+VIDAYLVDANG V + E+MGE LFWAIRGG
Sbjct: 181 GVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVV WKVKLV VP  VT C  +++L++    K++++WQYVA K++ NLL+GI+L
Sbjct: 241 GGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDK-NTKKIVYQWQYVANKMEENLLIGINL 300

Query: 301 T----DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGW 360
           T     +  K NP ASF  ++LGK ++++ I+  TFP LGLTK +C E SWIQS L+   
Sbjct: 301 TGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAG 360

Query: 361 FQKGDPLEVLLNRTRLYSEIS-KIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPY 420
           F  G PLE+LL +  L + IS KIKSDYVK+ I   A KG+W+RL+S +VE SQ+   PY
Sbjct: 361 FTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPY 420

Query: 421 GGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSP 480
           GGKM+ +   +TPFSHR+++ Y + Y   W      A + HLNWIRE+Y +MTPFVS SP
Sbjct: 421 GGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSP 480

Query: 481 RAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSI 540
           RAAYVNYRDLDIGTNNK GKT+Y+ A +WG+KYFGNNF RLV VKT VDPS+FFRH+QSI
Sbjct: 481 RAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSI 533

BLAST of PI0008720.1 vs. ExPASy TrEMBL
Match: A0A1S3BDG4 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488681 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.8e-217
Identity = 378/544 (69.49%), Postives = 437/544 (80.33%), Query Frame = 0

Query: 1   MKSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDIPIYTPINSSYSSIL 60
           MKSFPLNPLLF SFTVVSL STQWSTASSYKN DFL+CLSIHS DIPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYIS 120
           RSYAQNPRFSTQ+KTLKPL IIKPSHVSHIQSTVICSKSHGLQIRI SGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPA 180
           DLPFIVVDLINLKSI+VDTTNNT WVQSGATIGELYYRIA          EKS+TLAFPA
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIA----------EKSRTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           G CPTVGVGG  SGGGYGWL+RKYG+AAD+VIDAYLVDANG V + E+MGE LFWAIRGG
Sbjct: 181 GVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVV WKVKLV VP  VT C  +++L++    K++++WQYVA K++ NLL+GI+L
Sbjct: 241 GGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDK-NTKKIVYQWQYVANKMEENLLIGINL 300

Query: 301 T----DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGW 360
           T     +  K NP ASF  ++LGK ++++ I+  TFP LGLTK +C E SWIQS L+   
Sbjct: 301 TGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAG 360

Query: 361 FQKGDPLEVLLNRTRLYSEIS-KIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPY 420
           F  G PLE+LL +  L + IS KIKSDYVK+ I   A KG+W+RL+S +VE SQ+   PY
Sbjct: 361 FTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPY 420

Query: 421 GGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSP 480
           GGKM+ +   +TPFSHR+++ Y + Y   W      A + HLNWIRE+Y +MTPFVS SP
Sbjct: 421 GGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSP 480

Query: 481 RAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSI 540
           RAAYVNYRDLDIGTNNK GKT+Y+ A +WG+KYFGNNF RLV VKT VDPS+FFRH+QSI
Sbjct: 481 RAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSI 533

BLAST of PI0008720.1 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 3.9e-203
Identity = 358/550 (65.09%), Postives = 426/550 (77.45%), Query Frame = 0

Query: 1   MKSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDIP-----IYTPINSS 60
           MKSFPLNPL F     + L S+ +  AS++K+  FLQCL+ HS         IYTP N S
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  YSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEG 120
           YSSIL    QN RFST  +T KPL++I PSH+SHIQ+ VICSK+HGLQIR  SGGHD EG
Sbjct: 61  YSSILNFSIQNLRFSTA-ETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEG 120

Query: 121 LSYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQT 180
           LSY+S LPFIV+DLI+L+SI +D  N+TAWVQSGAT+GELYYRIA          +KSQT
Sbjct: 121 LSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIA----------QKSQT 180

Query: 181 LAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFW 240
           LAFPAGSCPTVGVGGHLSGGGYG+LIRKYG+AADHVIDA+LVDANG++ N+ESM +DLFW
Sbjct: 181 LAFPAGSCPTVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFW 240

Query: 241 AIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLL 300
            IRGGGGGSFGIVVAWK++LV VP  +T C  NRTL+   + KLIH WQYVA KL+ NLL
Sbjct: 241 TIRGGGGGSFGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGV-KLIHRWQYVAHKLEDNLL 300

Query: 301 LGIS------LTDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQ 360
           LGIS      LT + GK NP+ASF   FLGK +E++ I+  TFP+LGLTKEDC+E SWI+
Sbjct: 301 LGISLTGGHILTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIE 360

Query: 361 SVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQ 420
           S L+   F  G+ LE LL R  L +  +KIKSDYVKE I     KG+WERL +QD+EL+Q
Sbjct: 361 STLITAGFSAGESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQ 420

Query: 421 IMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTP 480
           +MFVPYGG+MSE  + ETP+ HR++  Y +GYI KW+  S +AEK HLNW+REIYDYM P
Sbjct: 421 VMFVPYGGRMSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAP 480

Query: 481 FVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFF 540
           +V+KSPRAAYVNYRDLDIG NN+Y KTNY+RA+VWGLKYFG NF+RLV VKT+VDPSDFF
Sbjct: 481 YVTKSPRAAYVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFF 538

BLAST of PI0008720.1 vs. NCBI nr
Match: XP_004135742.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653322.1 hypothetical protein Csa_023372 [Cucumis sativus])

HSP 1 Score: 902.5 bits (2331), Expect = 1.8e-258
Identity = 463/567 (81.66%), Postives = 486/567 (85.71%), Query Frame = 0

Query: 1   MKSFPLNP-----LLFISFTVVSLFSTQWSTASSYKNNDFLQCL----SIHSRDIP--IY 60
           MK FP+ P     + FI   VV L +   S  S+ ++  F+QCL    S  +  I   IY
Sbjct: 1   MKCFPILPAPFGLIAFIIIIVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60

Query: 61  TPINSSYSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSG 120
           TP NSSYSSIL    QN RF  +    KP  II P HVSHIQ+ VICSKSHG QIR  SG
Sbjct: 61  TPTNSSYSSILNFSIQNLRFLNK-DIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSG 120

Query: 121 GHDTEGLSYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRI 180
           GHD EGLSYISD+PF++VDLINLKSI +D  N  AWV SGAT+GELYYRI+         
Sbjct: 121 GHDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWVHSGATLGELYYRIS--------- 180

Query: 181 GEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESM 240
            EKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVHNKESM
Sbjct: 181 -EKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESM 240

Query: 241 GEDLFWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPK 300
           GEDLFWAIRGGGGGSFGIVVAWKVKLV VPARVTICEANRTLEE RILKLIHEWQYVAPK
Sbjct: 241 GEDLFWAIRGGGGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPK 300

Query: 301 LDANLLLGISLT------DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCL 360
           LDANLLLGISLT      DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCL
Sbjct: 301 LDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCL 360

Query: 361 EMSWIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQ 420
           EMSWIQSVLLMGWFQK DPLEVLLNR+RLYSEISKIKSDYVKEHIPMVA+KGMWERLKSQ
Sbjct: 361 EMSWIQSVLLMGWFQKEDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQ 420

Query: 421 DVELSQIMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREI 480
           DVELSQIMFVPYGGKMSEVDD ETPFSHRSDYSYLVGYIAKWKNGSVEAEK+HLNWIREI
Sbjct: 421 DVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREI 480

Query: 481 YDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKV 540
           YDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG+T+YKRARVWGLKYFG NFDRLVHVKTKV
Sbjct: 481 YDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKV 540

Query: 541 DPSDFFRHEQSIPTLSGISEESFSASL 551
           DPSDFFRHEQSIPTLSGIS+E FSASL
Sbjct: 541 DPSDFFRHEQSIPTLSGISKELFSASL 556

BLAST of PI0008720.1 vs. NCBI nr
Match: XP_008445757.1 (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo] >KAA0047085.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa] >TYK05070.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa])

HSP 1 Score: 901.7 bits (2329), Expect = 3.0e-258
Identity = 460/564 (81.56%), Postives = 490/564 (86.88%), Query Frame = 0

Query: 1   MKSFPL--NPLLFISFTVVSLFSTQWSTASSYKNNDFLQCL----SIHSRDIP--IYTPI 60
           MK FP+   P   I+F +V + ST  ++  + + + F+QCL    S +S  I   IYTP 
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSYSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHD 120
           NSSYSSIL    QN RF  ++ + KPL II PSHVSHIQ+ VICSKSHG QIR  SGGHD
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNIS-KPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120

Query: 121 TEGLSYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEK 180
            EGLSYIS +PF++VDLINL SI +D  N  AWV+SGAT+GELYYRIA          EK
Sbjct: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIA----------EK 180

Query: 181 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGED 240
           SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGKVHNKESMGED
Sbjct: 181 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGED 240

Query: 241 LFWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDA 300
           LFWAIRGGGGG FGIVVAWKVKLV VPARVTICEANRTLEE RILKLIHEWQYVAPKLDA
Sbjct: 241 LFWAIRGGGGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDA 300

Query: 301 NLLLGISLT------DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMS 360
           NLLLGISLT      DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQL LTKEDCLEMS
Sbjct: 301 NLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMS 360

Query: 361 WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVE 420
           WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIP+VALKGMWERLKS+DVE
Sbjct: 361 WIQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVE 420

Query: 421 LSQIMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDY 480
           LSQIMFVPYGGKMSEVDD ETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDY
Sbjct: 421 LSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDY 480

Query: 481 MTPFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPS 540
           MTPFVSKSPRAAYVNYRDLDIGTNNKYG+T+YKRARVWGLKYFG NFD+LV+VKTKVDPS
Sbjct: 481 MTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPS 540

Query: 541 DFFRHEQSIPTLSGISEESFSASL 551
           DFFRH+QSIPTLSGISEESFSASL
Sbjct: 541 DFFRHQQSIPTLSGISEESFSASL 553

BLAST of PI0008720.1 vs. NCBI nr
Match: XP_038895846.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 850.1 bits (2195), Expect = 1.0e-242
Identity = 425/561 (75.76%), Postives = 474/561 (84.49%), Query Frame = 0

Query: 1   MKSFPLN-PLLFISFTVVSLFSTQWSTASSYKNNDFLQC------LSIHSRDIPIYTPIN 60
           M+ FPL  PL  I+F +  + +T  ++  + +++ F+QC      L+ H     IYTPIN
Sbjct: 1   MECFPLTAPLGRIAFIIFIVLTTTTTSFCTQESDSFVQCLFDYGSLNTHPISDVIYTPIN 60

Query: 61  SSYSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDT 120
           SSYSS+L    QN RF T+ KT KPL II PSHVSHIQ+ VICSKSHGLQIR  SGGHD 
Sbjct: 61  SSYSSVLNFSVQNLRFLTK-KTPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDF 120

Query: 121 EGLSYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKS 180
           EGLSYISD PFIVVDLINL+SI +D  N  AWVQSGAT+GELYYRIA          EKS
Sbjct: 121 EGLSYISDAPFIVVDLINLRSIAIDVENENAWVQSGATLGELYYRIA----------EKS 180

Query: 181 QTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDL 240
           QTLAFPAGSC TVGVGGHLSGGGYGWLIRKYG+AADHVIDAYLVDANGK+H++ESMGEDL
Sbjct: 181 QTLAFPAGSCSTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKLHDRESMGEDL 240

Query: 241 FWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDAN 300
           FWAIRGGGGGSFGIVV+WKVKLV VPA VT+CEANRTLEE  ++KLIHEWQYVAPKLDAN
Sbjct: 241 FWAIRGGGGGSFGIVVSWKVKLVRVPATVTVCEANRTLEED-VMKLIHEWQYVAPKLDAN 300

Query: 301 LLLGISLT------DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSW 360
           LLLGISLT      DEEGKINPIASF   FLGKAEE++TILKPTF QLGL +EDCLE+SW
Sbjct: 301 LLLGISLTGGKVTEDEEGKINPIASFVAQFLGKAEELMTILKPTFSQLGLKREDCLEISW 360

Query: 361 IQSVLLMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVEL 420
           I+SVLLMGWFQK DP+EVLLNRTRLYSEISKIKSDYVKE IP  ++KGMWERLKSQDVEL
Sbjct: 361 IESVLLMGWFQKEDPIEVLLNRTRLYSEISKIKSDYVKERIPKASIKGMWERLKSQDVEL 420

Query: 421 SQIMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYM 480
           +QIMFVPYGG+MSEV +WETP+SHR DY YL+GYIAKWKNG+VEAEK HLNWIREIYDYM
Sbjct: 421 AQIMFVPYGGRMSEVYEWETPYSHRGDYMYLIGYIAKWKNGTVEAEKRHLNWIREIYDYM 480

Query: 481 TPFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSD 540
           TPFVS SPR AYVNYRDLDIGTNNKY KT+YKRAR WG+KYFGNNFDRLV++KTKVDPS+
Sbjct: 481 TPFVSNSPRVAYVNYRDLDIGTNNKYDKTSYKRARNWGMKYFGNNFDRLVYIKTKVDPSN 540

Query: 541 FFRHEQSIPTLSGISEESFSA 549
           FF+HEQSIP L+GI+EE FSA
Sbjct: 541 FFKHEQSIPILTGITEEFFSA 549

BLAST of PI0008720.1 vs. NCBI nr
Match: XP_004135741.1 (tetrahydrocannabinolic acid synthase [Cucumis sativus] >KAE8653321.1 hypothetical protein Csa_023149 [Cucumis sativus])

HSP 1 Score: 771.5 bits (1991), Expect = 4.7e-219
Identity = 380/544 (69.85%), Postives = 440/544 (80.88%), Query Frame = 0

Query: 1   MKSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDIPIYTPINSSYSSIL 60
           MK FPLNPL  ISFT+VSLFSTQWSTASSYKN DFLQCLSIHS  IPIYTPINSSYSSIL
Sbjct: 1   MKFFPLNPLFLISFTIVSLFSTQWSTASSYKNKDFLQCLSIHSTHIPIYTPINSSYSSIL 60

Query: 61  RSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYIS 120
           RSYAQN RFSTQ+KTLKPLVIIKPSHVSH+QSTVICSKSH LQIRI SGGHDTEGLSYIS
Sbjct: 61  RSYAQNSRFSTQYKTLKPLVIIKPSHVSHLQSTVICSKSHDLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPA 180
           DLPF+VVDLINLKSI+VDTTNNTAWVQSGATIGELYYRIA          EKS+TLAFPA
Sbjct: 121 DLPFVVVDLINLKSIEVDTTNNTAWVQSGATIGELYYRIA----------EKSRTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           G CPTVG+GGH SGGGYGWL+RKYG+AAD+VIDAYLVDANG V ++E+MGEDLFWAIRGG
Sbjct: 181 GVCPTVGIGGHFSGGGYGWLMRKYGLAADNVIDAYLVDANGGVFDREAMGEDLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVV WKVKLV VP  VT C  +++L++    K++++WQYVA +++  LL+GI+L
Sbjct: 241 GGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDK-NTKKIVYQWQYVANRMEEKLLIGINL 300

Query: 301 T----DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGW 360
           T     +  K NP ASF  ++LGK ++++TI+  T P LGLTK +C E SWIQS L+   
Sbjct: 301 TGGNPTKGVKRNPTASFFSLYLGKTDKLVTIMNTTLPNLGLTKANCKETSWIQSTLIAAG 360

Query: 361 FQKGDPLEVLLNRTRLYSEIS-KIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPY 420
           F  G PLE+LL++  L + IS KIKSDYVK+ I   A KG+W+RLKSQ+VE SQ+   PY
Sbjct: 361 FTNGQPLEILLSKPTLSNNISYKIKSDYVKQPISQHAFKGIWDRLKSQEVETSQLFLFPY 420

Query: 421 GGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSP 480
           GGKMS +   +TPFSHR+++ Y + Y   W      A + HLNWIRE Y +MTPFVS SP
Sbjct: 421 GGKMSNISSSKTPFSHRAEFLYKISYTVGWAEQGSGANERHLNWIREFYSFMTPFVSNSP 480

Query: 481 RAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSI 540
           RAAYVNYRDLDIGTNNKYGKT+Y+ A +WGLKYFGNNF +LV VKT VDPS+FFRHEQSI
Sbjct: 481 RAAYVNYRDLDIGTNNKYGKTSYEEASIWGLKYFGNNFKKLVQVKTTVDPSNFFRHEQSI 533

BLAST of PI0008720.1 vs. NCBI nr
Match: XP_008445749.1 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >KAA0047086.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa] >TYK05069.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 764.6 bits (1973), Expect = 5.8e-217
Identity = 378/544 (69.49%), Postives = 437/544 (80.33%), Query Frame = 0

Query: 1   MKSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDIPIYTPINSSYSSIL 60
           MKSFPLNPLLF SFTVVSL STQWSTASSYKN DFL+CLSIHS DIPIYTPINSSY+S L
Sbjct: 1   MKSFPLNPLLFTSFTVVSLSSTQWSTASSYKNKDFLRCLSIHSTDIPIYTPINSSYTSTL 60

Query: 61  RSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYIS 120
           RSYAQNPRFSTQ+KTLKPL IIKPSHVSHIQSTVICSKSHGLQIRI SGGHDTEGLSYIS
Sbjct: 61  RSYAQNPRFSTQYKTLKPLAIIKPSHVSHIQSTVICSKSHGLQIRIRSGGHDTEGLSYIS 120

Query: 121 DLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPA 180
           DLPFIVVDLINLKSI+VDTTNNT WVQSGATIGELYYRIA          EKS+TLAFPA
Sbjct: 121 DLPFIVVDLINLKSIEVDTTNNTVWVQSGATIGELYYRIA----------EKSRTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           G CPTVGVGG  SGGGYGWL+RKYG+AAD+VIDAYLVDANG V + E+MGE LFWAIRGG
Sbjct: 181 GVCPTVGVGGQFSGGGYGWLMRKYGLAADNVIDAYLVDANGMVFDSEAMGEGLFWAIRGG 240

Query: 241 GGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGISL 300
           GGGSFGIVV WKVKLV VP  VT C  +++L++    K++++WQYVA K++ NLL+GI+L
Sbjct: 241 GGGSFGIVVGWKVKLVRVPPIVTTCRLDKSLDK-NTKKIVYQWQYVANKMEENLLIGINL 300

Query: 301 T----DEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGW 360
           T     +  K NP ASF  ++LGK ++++ I+  TFP LGLTK +C E SWIQS L+   
Sbjct: 301 TGGNPTKGVKRNPTASFFSLYLGKTDKLIKIMNTTFPNLGLTKANCKETSWIQSTLIAAG 360

Query: 361 FQKGDPLEVLLNRTRLYSEIS-KIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPY 420
           F  G PLE+LL +  L + IS KIKSDYVK+ I   A KG+W+RL+S +VE SQ+   PY
Sbjct: 361 FTNGQPLEILLTKPTLSNNISYKIKSDYVKQPISEHAFKGIWDRLQSHEVETSQLFLFPY 420

Query: 421 GGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKSP 480
           GGKM+ +   +TPFSHR+++ Y + Y   W      A + HLNWIRE+Y +MTPFVS SP
Sbjct: 421 GGKMNNISSSKTPFSHRAEFLYKISYTVGWAEQGTGANERHLNWIRELYSFMTPFVSNSP 480

Query: 481 RAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSI 540
           RAAYVNYRDLDIGTNNK GKT+Y+ A +WG+KYFGNNF RLV VKT VDPS+FFRH+QSI
Sbjct: 481 RAAYVNYRDLDIGTNNKCGKTSYEEASIWGMKYFGNNFKRLVQVKTMVDPSNFFRHQQSI 533

BLAST of PI0008720.1 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 494.6 bits (1272), Expect = 1.0e-139
Identity = 252/542 (46.49%), Postives = 356/542 (65.68%), Query Frame = 0

Query: 2   KSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSRDI-----PIYTPINSSY 61
           +SF  + L+F + + + L      +A+    + FLQCLS+   D       I+TP ++S+
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 63

Query: 62  SSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGL 121
           SS+L S  QN RFS      KP++I+ P   S +QS V C++  G+ IR  SGGHD EGL
Sbjct: 64  SSVLASSIQNQRFSAP-DVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGL 123

Query: 122 SYISDLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTL 181
           SY++  PF+++DL NL+SI VD  N + WVQ+GATIG          ELYY IG+K++TL
Sbjct: 124 SYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIG----------ELYYEIGKKNRTL 183

Query: 182 AFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWA 241
           AFPAG CPTVGVGGH SGGGYG L+RK+G+AADHVIDA +VDA G++  +  MGED FWA
Sbjct: 184 AFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWA 243

Query: 242 IRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLL 301
           IRGGGG SF +V++WK+ L+ VP+ VT+    +  E+   LK+IH WQ+VA K+  +L +
Sbjct: 244 IRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQS-ALKIIHRWQFVADKVSDDLFI 303

Query: 302 GISLTDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGW 361
            + L   +  +   ASF  ++LG  + +L ++   FP+LGL ++DC EMSWI+SV+   W
Sbjct: 304 RVMLQRYKNMVR--ASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVI---W 363

Query: 362 FQK--GDPLEVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVP 421
           F +   +P+ VL  RTR  S   K KSD+V+E +P  A+  +W RL+  + E +Q++F P
Sbjct: 364 FAELGEEPINVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTP 423

Query: 422 YGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDYMTPFVSKS 481
           +GGKMSE+ D+ETPF HR    Y + Y+  W+    + ++ ++ W+  +YD M+ FV+KS
Sbjct: 424 FGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKS 483

Query: 482 PRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQS 537
           PR AY+N RDLD+G      ++ Y+  + WG+KYF NNF+RLV VKT VDPSDFF  EQS
Sbjct: 484 PRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQS 524

BLAST of PI0008720.1 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.8e-136
Identity = 257/545 (47.16%), Postives = 360/545 (66.06%), Query Frame = 0

Query: 10  LFISFTVVSLFSTQWSTASSYKNN--DFLQCLS--IHSRDIP-IYTPINSSYSSILRSYA 69
           LFI F +++L  + + + S+ + N  +FL+CLS  I+  D   I+T  + SY SIL S  
Sbjct: 10  LFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHRINEDDSRIIHTSKDPSYFSILNSSI 69

Query: 70  QNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYIS-DLP 129
           QNPRF    +T KP+ II P   + +QST+ C++ HG+ IR  SGGHD EGLSY++   P
Sbjct: 70  QNPRFFVL-ETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRP 129

Query: 130 FIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPAGSC 189
           F+V+DL NL+SI +D  N T WVQSGATIG          ELYY IG+ S++LAFPAG  
Sbjct: 130 FVVIDLRNLRSITLDVDNRTGWVQSGATIG----------ELYYEIGKLSKSLAFPAGLY 189

Query: 190 PTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 249
           PTVG+GG   GGGYG L+RKYG++AD+VIDA++VDANG   +++ MGED FWAIRGGGG 
Sbjct: 190 PTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGS 249

Query: 250 SFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGISLTDE 309
           SF +V++WK++L+ VP+ VT+ +  +T E+   + +I++WQY+A K+  +L +   L  E
Sbjct: 250 SFSVVLSWKIRLLDVPSVVTVFKVVKTSEK-EAVSIINKWQYIADKVPNDLFIRAMLQKE 309

Query: 310 EGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDPL 369
                  ASF  ++LG   ++L ++K  FP+LGL   +C EMSWI+SVL   WF KG+ +
Sbjct: 310 ---TEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESM 369

Query: 370 EVLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPYGGKMSEVD 429
           E+L  R R  S   K K D+++E IP  A++ +W R ++ +  L++I+  P+GGKMSE+ 
Sbjct: 370 EILAKRKRT-SRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 429

Query: 430 DWETPFSHRSDYSYLVGYIAKWK----NGSVEAEKTHLNWIREIYDYMTPFVSKSPRAAY 489
           D E PF HR    Y + Y+A W           EK +L W+  +Y++MTP+VSKSPR AY
Sbjct: 430 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEK-YLRWVESVYEFMTPYVSKSPRRAY 489

Query: 490 VNYRDLDIGTNNKYG-KTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSIPTL 544
           VN+RD+D+G       KT Y+ A+VWG+KYF NNFDRLV VKT VDP DFF  EQSIP +
Sbjct: 490 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 534

BLAST of PI0008720.1 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.2e-135
Identity = 252/542 (46.49%), Postives = 360/542 (66.42%), Query Frame = 0

Query: 14  FTVVSLFSTQWS---TASSYKNNDFLQCLSIHSRDIP-----IYTPINSSYSSILRSYAQ 73
           FT++ LFS   S   +A    + DFL+CLS    D       I+T  +SS+ SIL S  Q
Sbjct: 8   FTIL-LFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQ 67

Query: 74  NPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTEGLSYIS-DLPF 133
           NPRFS   +T KP+ II P   S +Q+ + C++ HG+ +R  S GH  EGLSYI+ + PF
Sbjct: 68  NPRFSVS-ETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPF 127

Query: 134 IVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEKSQTLAFPAGSCP 193
            V+DL NL+SI +D  N T WVQ+GAT G          ELYY IG+ +++LAFPAG  P
Sbjct: 128 AVIDLRNLRSISLDVDNRTGWVQTGATAG----------ELYYEIGKTTKSLAFPAGIHP 187

Query: 194 TVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGS 253
           TVGVGG  SGGGYG L+RKYG+AAD++IDA +VDA+G++ ++++MGED FWAIRGGGG S
Sbjct: 188 TVGVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSS 247

Query: 254 FGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLDANLLLGISLTDEE 313
           FG++++WKVKLV VP+ +T+ +  +T ++   +++I +WQY A K+  +L +  +L +  
Sbjct: 248 FGVILSWKVKLVDVPSTITVFKVQKTSKK-EAVRIIKKWQYAADKVPDDLFIRTTL-ERS 307

Query: 314 GKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLMGWFQKGDPLE 373
            K    A F+ +++G    +L +++  FP+LGL KE C EMSWI+SVL    F KG+ L 
Sbjct: 308 NKNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLG 367

Query: 374 VLLNRTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQIMFVPYGGKMSEVDD 433
           VL NR R  S   K K D+V+E IP  A++ +W RL++ +  L +I+  P+GGKMSE+ +
Sbjct: 368 VLTNRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAE 427

Query: 434 WETPFSHRSDYSYLVGYIAKWK----NGSVEAEKTHLNWIREIYDYMTPFVSKSPRAAYV 493
           +ETPF HR    Y + Y+A W+        E +K +L W+  +Y++MTP+VSKSPR AYV
Sbjct: 428 YETPFPHRGGNLYEIQYVAYWREEEDKNKTETDK-YLKWVDSVYEFMTPYVSKSPRGAYV 487

Query: 494 NYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDFFRHEQSIPTLSG 543
           N++D+D+G      KT Y+  + WG+KYF NNF+RLV VKT+VDP+DFF  EQSIP ++ 
Sbjct: 488 NFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVNK 533

BLAST of PI0008720.1 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 480.3 bits (1235), Expect = 2.0e-135
Identity = 249/552 (45.11%), Postives = 363/552 (65.76%), Query Frame = 0

Query: 3   SFPLNPLLFIS-FTVVSLFSTQWSTASSYKNNDFLQCLSIHSR-----DIPIYTPIN--S 62
           S PL  +  IS F +   F T   T+S+   +DF++CL  ++      D   +TP    S
Sbjct: 4   SKPLPTISCISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNAS 63

Query: 63  SYSSILRSYAQNPRFSTQHKTL-KPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDT 122
            ++ +L S AQN R+ T  KT+ KP  I KP H SH+Q++VICSK   +  R+ SGGHD 
Sbjct: 64  IFTEVLESTAQNQRYLT--KTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDY 123

Query: 123 EGLSYISDL--PFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGE 182
           EG+SY+S +  PF+++DL  L+ I VD  + +AWV++GAT+GELYYRIAEKS+ +     
Sbjct: 124 EGVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFH----- 183

Query: 183 KSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGE 242
                 FPAG  P++G+GGH++GG YG L+RKYG+AAD+V+DA +VDANGK+ ++ SMGE
Sbjct: 184 -----GFPAGVYPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGE 243

Query: 243 DLFWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKLD 302
           DLFWAIRGG GGSFGI+++WK+KLV VP  +T+    +T E+ R  K++ +WQ +A  L 
Sbjct: 244 DLFWAIRGGSGGSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLV 303

Query: 303 ANLLLGISLTDEEGKINPIASFSLM--FLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQ 362
             L L +  T    K N   + + +  FLG+   ++ ++K  FP+LGLT++DC+EMSWI 
Sbjct: 304 DELFLRVFFTVSGNKANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWID 363

Query: 363 SVLLMGWFQKG--DPLEVLLN-RTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVE 422
           S++    F      P+E+LL  ++ +     K KSD+ K+ IP++ L+GM+++L  +D  
Sbjct: 364 SIIYNSGFPTNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAA 423

Query: 423 LSQIMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKWKNGSVEAEKTHLNWIREIYDY 482
           L  +++ PYGGKM ++ + E PF HR+  ++++ Y   W + S +       WIRE+Y Y
Sbjct: 424 L--VIWTPYGGKMDKIPESEIPFPHRNGTNFMIQYYRSWSD-SEKRPNRRTKWIRELYGY 483

Query: 483 MTPFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPS 539
           MTP+VS +PR AYVNYRDLD+G N    K+N+  A++WG  YF +NF+RLV +K+KVDP 
Sbjct: 484 MTPYVSSNPRQAYVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPD 540

BLAST of PI0008720.1 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.5e-133
Identity = 243/550 (44.18%), Postives = 354/550 (64.36%), Query Frame = 0

Query: 2   KSFPLNPLLFISFTVVSLFSTQWSTASSYKNNDFLQCLSIHSR-----DIPIYTPIN--S 61
           K  P    + +S    S +    +++S+   + F+ C+  ++      +  ++TP    S
Sbjct: 5   KPLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCVKRNTHVSFPLEKTLFTPAKNVS 64

Query: 62  SYSSILRSYAQNPRFSTQHKTLKPLVIIKPSHVSHIQSTVICSKSHGLQIRIHSGGHDTE 121
            ++ +L S AQN +F  +    KP  I +P H S +Q+++ICSK  G+  R+ SGGHD E
Sbjct: 65  LFNQVLESTAQNLQFLAKSMP-KPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFE 124

Query: 122 GLSYIS--DLPFIVVDLINLKSIKVDTTNNTAWVQSGATIGELYYRIAEKSELYYRIGEK 181
            LSY+S  + PFI++DL  LK I VD  +N+AWVQ GAT+GELYYRIAEKS+++      
Sbjct: 125 ALSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIH------ 184

Query: 182 SQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGVAADHVIDAYLVDANGKVHNKESMGED 241
                FPAG C +VG+GG+++GGGYG L+RKYG+A D+V+D  +VDANGK+ ++ +MGED
Sbjct: 185 ----GFPAGLCTSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGED 244

Query: 242 LFWAIRGGGGGSFGIVVAWKVKLVGVPARVTICEANRTLEEGRILKLIHEWQYVAPKL-- 301
           LFWAIRGGGG SFGIV+AWK+KLV VP  VT+    +TLE+   LK I +WQ ++ K+  
Sbjct: 245 LFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIE 304

Query: 302 DANLLLGISLTDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQS 361
           + ++ + +     +G      ++   FLG+   +L +++  FP+LGLT++DC EMSWI++
Sbjct: 305 EIHIRVVLRAAGNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEA 364

Query: 362 VLLMGWFQKGDPLEVLLN-RTRLYSEISKIKSDYVKEHIPMVALKGMWERLKSQDVELSQ 421
            L  G F  G P+E+LL  ++ L  +  K  SD+VKE IP++ LKG+++RL   +     
Sbjct: 365 ALFHGGFPTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF-- 424

Query: 422 IMFVPYGGKMSEVDDWETPFSHRSDYSYLVGYIAKW-KNGSVEAEKTHLNWIREIYDYMT 481
           + + PYGG MS++ +   PF HR+   + + Y A W +N    + K  +NWI+EIY+YM 
Sbjct: 425 LNWTPYGGMMSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRK--INWIKEIYNYMA 484

Query: 482 PFVSKSPRAAYVNYRDLDIGTNNKYGKTNYKRARVWGLKYFGNNFDRLVHVKTKVDPSDF 539
           P+VS +PR AYVNYRDLD G N    K N+  A++WG KYF  NFDRLV +KTKVDP +F
Sbjct: 485 PYVSSNPRQAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENF 539