PI0008652.1 (mRNA) Melon (PI 482460) v1

BLAST of PI0008652.1 vs. ExPASy Swiss-Prot
Match: Q9T0J6 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)

HSP 1 Score: 873.2 bits (2255), Expect = 1.9e-252
Identity = 437/677 (64.55%), Postives = 538/677 (79.47%), Query Frame = 0

Query: 32  TPQQLVAKAIAPVKRDFLRPPPLRSSSNNVKDATSTAKNDGDDKPAP-STLVKEKKSKRQ 91
           T ++L+ +++AP+K++FL PPP          + S  +ND  D  AP S LV+EKKSKRQ
Sbjct: 36  TSEELIERSMAPIKKEFLCPPP---------PSRSVKQNDAADVRAPQSGLVQEKKSKRQ 95

Query: 92  LKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGP 151
           LKRERR++  ST +LCP++A+T DV SC+Y DKCRF+HDIEAFK+QK  D+EG+CPF   
Sbjct: 96  LKRERREQ--STINLCPQVARTEDVDSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVAS 155

Query: 152 EQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLK 211
              C YGL+CRF G+H++    G+S   K  SEMN  NK+ Q+LLWKNKM F  AD+KLK
Sbjct: 156 GMKCAYGLSCRFLGSHRD--ITGNSDD-KEKSEMNFFNKETQRLLWKNKMTFTNADAKLK 215

Query: 212 ELGLLGHGKSKLRVKE---DKENAVSNDSVVAERTCCSEMNNDLDADLEISVEVPEENGE 271
            LGLLGH K     +E   +K     N +   E    S ++++  +++   V++P     
Sbjct: 216 SLGLLGHAKKSNAAEEITAEKTQNGMNGTQATEVAVDSAVSSEHTSEMIQDVDIP----- 275

Query: 272 KEGTFSSDESRPLKKSKSLSSENFHSDQID---NGACMIDETIGEGHAPNKSEVMN-DSV 331
             G   ++E RP+KK+KS   +N  +  +    +G  + +ET   G+  +K+ V + DS+
Sbjct: 276 --GPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSI 335

Query: 332 -LLDSEASLKPHPRE-KKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTN 391
            ++++++SLK HPRE KKLIDFRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTN
Sbjct: 336 KIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTN 395

Query: 392 LLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIV 451
           LLQGQASEWALLRRHSSEDLFGVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+V
Sbjct: 396 LLQGQASEWALLRRHSSEDLFGVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMV 455

Query: 452 VNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASA 511
           VNK AGSALL KP+RMK +VE +S IV+TPITIKVRT ++EGKNRIDSLIADIGNWGA+A
Sbjct: 456 VNKSAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATA 515

Query: 512 VTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNQHKDECPKLATCM 571
           VTIHGR+RQQRYSK ADW+YI +C+  A  +LQVIGNGDV+SY DWN+HK +CP+L++CM
Sbjct: 516 VTIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCM 575

Query: 572 IARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLEHWGSDSKGVETTRHFLLEW 631
           IARGALIKPW+FTEIKEQR+WDITS ERLNI+KDFV FGL+HWGSD+KGVETTRHFLLEW
Sbjct: 576 IARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEW 635

Query: 632 LSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGF 691
           LSYT RYIPVGLL+V+PQ++NWRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+GF
Sbjct: 636 LSYTFRYIPVGLLDVIPQQINWRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGF 691

Query: 692 SFAPKHKSNAYDRAENG 699
           +FAPKHKSNAYDRAENG
Sbjct: 696 TFAPKHKSNAYDRAENG 691

BLAST of PI0008652.1 vs. ExPASy Swiss-Prot
Match: Q7XT07 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)

HSP 1 Score: 867.1 bits (2239), Expect = 1.4e-250
Identity = 435/685 (63.50%), Postives = 519/685 (75.77%), Query Frame = 0

Query: 22  SHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNVKDATSTAKNDGDDKPAPSTL 81
           S +A +    +P++LVA+A+APVK  FLRPP            ++T   D         +
Sbjct: 20  SPAASSPPRPSPEELVARAVAPVKPAFLRPP-----------LSATPPKDEGKANGGGAV 79

Query: 82  VKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADL 141
           V EKKSKRQLKRER+QEQKS+ HLC E+ K+G+VSSC+Y D CRFSHDI+A+ +QKPADL
Sbjct: 80  VAEKKSKRQLKRERKQEQKSSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPADL 139

Query: 142 EGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMK 201
           EG CPF+  +QLCPYGL CRF GTHK+   A  +++ K   E+N LNKDIQKLLWKNK K
Sbjct: 140 EGTCPFTNLDQLCPYGLTCRFLGTHKDIHAASGNLSEKH--EINALNKDIQKLLWKNKYK 199

Query: 202 FPKADSKLKELGLLGHGKSKLRVKED-----KENAVSNDSVVAERTCCSEMNNDLDADLE 261
           FPKA +++K LGL    KSK     D      +N   ND    E  C   +N + D+ L 
Sbjct: 200 FPKASAQIKLLGLKEVIKSKPDAANDDKKVNHDNLDGNDDENKEPLCNPPVNAECDSTLC 259

Query: 262 ISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACMIDETIGEGHAPNKS 321
             ++  E     + +    E RP KKSK        SD+ID        T  E   PN S
Sbjct: 260 EELDRSEGEPLIDNSIPCVEPRPTKKSK------VESDEIDKHGAGTLNTNTESEDPNLS 319

Query: 322 ---EVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 381
              E  N+S    ++    PH REKK+IDFR+KLYLAPLTTVGNLPFRR+CK LGAD+TC
Sbjct: 320 NGLEPSNNSSSCRTDLITTPHLREKKIIDFREKLYLAPLTTVGNLPFRRLCKTLGADITC 379

Query: 382 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDIN 441
           GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+R VEL+D EC +DFIDIN
Sbjct: 380 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARTVELVDNECSVDFIDIN 439

Query: 442 MGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIAD 501
           MGCPIDIVVNKGAGS+LLTKPMR+K++V+AAS + + P+T+KVRT ++EG+NR DS+++D
Sbjct: 440 MGCPIDIVVNKGAGSSLLTKPMRIKSIVQAASTVTEKPLTVKVRTAFFEGRNRADSIVSD 499

Query: 502 IGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNQHKDE 561
           I +WGASA+T+HGR+RQQRYSKLADW+YI +C+  APD L V+GNGDVFS+TDWN+H   
Sbjct: 500 IYDWGASAITVHGRSRQQRYSKLADWDYIYQCAQKAPDQLHVVGNGDVFSFTDWNKHVSG 559

Query: 562 CPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLEHWGSDSKGVET 621
           C K++T MIARGALIKPW+FTE+KEQR+WDITS ER NILKDFV FGLEHWGSDSKGVET
Sbjct: 560 CSKISTSMIARGALIKPWIFTEVKEQRHWDITSGERFNILKDFVSFGLEHWGSDSKGVET 619

Query: 622 TRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 681
           TR+FLLEWLSYTCRYIPVGLL+V+PQRLNWRPPSY GRDDLETLM SDSAADWI+ISEML
Sbjct: 620 TRYFLLEWLSYTCRYIPVGLLDVIPQRLNWRPPSYCGRDDLETLMISDSAADWIRISEML 679

Query: 682 LGKVPDGFSFAPKHKSNAYDRAENG 699
           LGKVP+GF+F PKHKSNAYDRAENG
Sbjct: 680 LGKVPEGFTFTPKHKSNAYDRAENG 685

BLAST of PI0008652.1 vs. ExPASy Swiss-Prot
Match: Q91XI1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus3l PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.4e-146
Identity = 279/636 (43.87%), Postives = 381/636 (59.91%), Query Frame = 0

Query: 73  DDKPAPSTLVKEKKSKRQLKRERRQEQKSTRH----------LCPEIAKTGDVSSCRYSD 132
           D +    T V  +  +RQ+ +  R + KS  H          LCP   +    + C + D
Sbjct: 70  DGQENGKTEVAIESRERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQE-PATPCAFGD 129

Query: 133 KCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTH--KEGVPAGSSVTLKR 192
           +CRF HD+  +   KPADL   C        CP+ + CRFAG H   EG        + R
Sbjct: 130 RCRFLHDVGRYLETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVAR 189

Query: 193 CSEM----NGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDS 252
           C+++    NGL++ +Q+ L K ++ F +A+  L  L                       +
Sbjct: 190 CAQLPSVRNGLDRALQQQLRKRQVCFERAEQALNRL-----------------TQSPMPT 249

Query: 253 VVAERTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQI 312
           VV E T              +++  P++N                        + H+ Q+
Sbjct: 250 VVPETT--------------VAMATPKQN------------------------SCHA-QL 309

Query: 313 DNGACMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVG 372
           D        T+G    P  S V     L D +  ++  P EKK +D   KLYLAPLTT G
Sbjct: 310 D--------TVGGAGTPQSSPVPTCGPLTDEDV-IRLRPCEKKRLDISGKLYLAPLTTCG 369

Query: 373 NLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSR 432
           NLPFRR+CK  GADVTCGEMA+CTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PDT+++
Sbjct: 370 NLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAFPDTMTK 429

Query: 433 AVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKV 492
             EL+++   +DF+DIN+GCPID+V  KG G AL+ +  + + +V   +G++D P+T+K+
Sbjct: 430 CAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGVNGVLDVPLTVKM 489

Query: 493 RTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVI 552
           RTG  E  +    L+ ++ +WG + VT+HGR+R+QRY++LADW YI +C+ +A   + + 
Sbjct: 490 RTGVQERVSLAHRLLPELRDWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA-SPMPLF 549

Query: 553 GNGDVFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDF 612
           GNGD+ S+ D N        +A  M+ARGAL+KPWLFTEIKEQR+WDI+S+ERL+IL+DF
Sbjct: 550 GNGDILSFEDANCAMQ--TGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDF 609

Query: 613 VHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLET 672
            H+GLEHWGSD++GVE TR FLLEWLS+ CRY+PVGLLE  PQR+N RPP Y GRD LET
Sbjct: 610 THYGLEHWGSDTQGVERTRRFLLEWLSFLCRYVPVGLLERPPQRINERPPYYLGRDYLET 636

Query: 673 LMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 693
           LM+S  AADWI+ISEMLLG VP GF F PKHK+NAY
Sbjct: 670 LMASQQAADWIRISEMLLGPVPPGFVFLPKHKANAY 636

BLAST of PI0008652.1 vs. ExPASy Swiss-Prot
Match: Q3KRC5 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus3l PE=2 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 4.1e-146
Identity = 280/636 (44.03%), Postives = 378/636 (59.43%), Query Frame = 0

Query: 73  DDKPAPSTLVKEKKSKRQLKRERRQEQKSTRH----------LCPEIAKTGDVSSCRYSD 132
           D +    T V  +  +RQ+ +  R + KS  H          LCP + +    + C + D
Sbjct: 73  DGQENGKTEVAVESHERQVPKRARGQNKSRPHMKPAHYDKERLCPSLLQE-SATPCAFGD 132

Query: 133 KCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTH--KEGVPAGSSVTLKR 192
           +CRF HD+  +   KPADL   C        CPY + CRFAG H   EG        + R
Sbjct: 133 RCRFLHDVGRYLETKPADLGPHCVLFNTFGRCPYSMTCRFAGAHLGPEGQNLVQEEVVAR 192

Query: 193 CSEM----NGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDS 252
           C+++    NGL++ +Q+ L K ++ F +A+  L  L             +     ++ +S
Sbjct: 193 CAQLPSVRNGLDRALQQQLRKRQVCFERAEQALSHL------------TQGPMPTIAPES 252

Query: 253 VVAERTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQI 312
            VA  T                                    P   S  +        Q+
Sbjct: 253 TVATLT------------------------------------PKHSSCHV--------QL 312

Query: 313 DNGACMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVG 372
           DN        +G   A   S V     L D +  ++  P EKK +D   KLYLAPLTT G
Sbjct: 313 DN--------VGGDGARQGSPVPTCGPLTDEDV-VRLRPCEKKRLDISGKLYLAPLTTCG 372

Query: 373 NLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSR 432
           NLPFRR+CK  GADVTCGEMAMCTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PDT+++
Sbjct: 373 NLPFRRICKRFGADVTCGEMAMCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAFPDTMTK 432

Query: 433 AVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKV 492
             EL+++   +DF+DIN+GCPID+V  KG G AL+ +  + + +V   + ++D P+T+K+
Sbjct: 433 CAELLNRTIDVDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGMNEVLDVPLTVKM 492

Query: 493 RTGYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVI 552
           RTG  E  +    L+ ++ NWG + VT+HGR+R+QRY++LADW YI +C+ +A   + + 
Sbjct: 493 RTGVQERVSLAHRLLPELRNWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA-SPMPLF 552

Query: 553 GNGDVFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDF 612
           GNGD+ S+ D N        +A  M+ARGAL+KPWLFTEIKEQR+WDI+S+ERL+IL+DF
Sbjct: 553 GNGDILSFEDANCAMQ--TGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDF 612

Query: 613 VHFGLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLET 672
            H+GLEHWGSD++GVE TR FLLEWLS+ CRY+PVGLLE +PQR+N RPP Y GRD LET
Sbjct: 613 THYGLEHWGSDTQGVERTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYLET 639

Query: 673 LMSSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 693
           LM+S  AADWI+ISEMLLG VP GF F PKHK+NAY
Sbjct: 673 LMASQQAADWIRISEMLLGPVPPGFVFLPKHKANAY 639

BLAST of PI0008652.1 vs. ExPASy Swiss-Prot
Match: Q7ZWS1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus laevis OX=8355 GN=dus3l PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.7e-144
Identity = 283/627 (45.14%), Postives = 381/627 (60.77%), Query Frame = 0

Query: 70  NDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTR----HLCPEIAKTGDVSSCRYSDKCR 129
           ++G D+P  +   K++K + + + + R   K ++     LCP + +    S C + DKC+
Sbjct: 89  SEGQDQPPKTAENKQEKKRARGQNKSRPHMKHSQFEENKLCPSVTQE-CASKCFFGDKCK 148

Query: 130 FSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMN 189
           FSHD+  + SQKP D+   C        C YG+ CRFA +H  G    + +  +   E  
Sbjct: 149 FSHDVAKYVSQKPEDIRPNCHLYETFGKCIYGVTCRFAKSHL-GEDFKNIINEELMKEWE 208

Query: 190 GLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAERTCCS 249
           G      K+L KN +     D  LKE         +LR K+          VV E++   
Sbjct: 209 G------KVLVKNSL-----DKSLKE---------QLRKKK----------VVFEKS--- 268

Query: 250 EMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACMIDE 309
                 D  L++          K G  SS    P+ K  S          I     + DE
Sbjct: 269 ------DKYLKLCF--------KSGD-SSKMKNPVVKEDSAVQVTQKDSPITTVGAVTDE 328

Query: 310 TIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCK 369
            +                       +K  P EKK IDFR+KLYLAPLTT GNLPFRR+CK
Sbjct: 329 DV-----------------------IKLRPCEKKTIDFRNKLYLAPLTTCGNLPFRRICK 388

Query: 370 VLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKEC 429
            LGAD+TCGEMAMCTNLLQGQ SEWALL+RH SED+FGVQ+ GA+PDT+++  EL+++  
Sbjct: 389 RLGADITCGEMAMCTNLLQGQPSEWALLKRHHSEDIFGVQLEGAFPDTMTKCAELLNRTI 448

Query: 430 VLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKN 489
            +DF+DIN+GCPID+V  KG G  L+ +  + + +V+  + ++D P+T+K+RTG  E  N
Sbjct: 449 DVDFVDINVGCPIDLVYKKGGGCGLMNRTNKFEQIVKGMNSVLDVPLTVKIRTGVQEKVN 508

Query: 490 RIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYT 549
               LI ++ +WG S VT+HGR+R+QRY+KLA+WEYI++C+ +A   + + GNGD+ SY 
Sbjct: 509 IAHKLIPNLRDWGVSLVTLHGRSREQRYTKLANWEYIDQCAKIA-SPVPLFGNGDIISYE 568

Query: 550 DWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLEHWG 609
           D N+       +A  M+ARGAL KPWLFTEIKEQR+WDI+S ER +ILKDF ++GLEHWG
Sbjct: 569 DANRALQ--TGVAGVMLARGALFKPWLFTEIKEQRHWDISSTERFDILKDFTNYGLEHWG 628

Query: 610 SDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAAD 669
           SD +GVE TR F+LEWLS+ CRYIPVGLLE VPQ++N RPP Y GRD LETLM+S +  D
Sbjct: 629 SDCQGVEKTRKFMLEWLSFLCRYIPVGLLEHVPQKINERPPYYMGRDYLETLMASQNVTD 639

Query: 670 WIKISEMLLGKVPDGFSFAPKHKSNAY 693
           WIKISEMLLG VP  F+F PKHK+N+Y
Sbjct: 689 WIKISEMLLGPVPPNFTFLPKHKANSY 639

BLAST of PI0008652.1 vs. ExPASy TrEMBL
Match: A0A0A0K4I1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 679/698 (97.28%), Postives = 689/698 (98.71%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDSVTADLLDD AVS+ P+SHS+DNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN
Sbjct: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTAKNDGDDK APSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY
Sbjct: 61  LKDATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKED+ENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           R CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA
Sbjct: 241 RPCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAP +SE MNDSVLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFG 600
           VFSYTDWN+HK+ECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDF HFG
Sbjct: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of PI0008652.1 vs. ExPASy TrEMBL
Match: A0A5D3CK47 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G001380 PE=3 SV=1)

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 667/698 (95.56%), Postives = 675/698 (96.70%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDS+T       AVS+ P+SHSADNGTH TPQQLVAKAIAPV  DFLRPPPLRSSSN 
Sbjct: 1   MEEDSLT-------AVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTA NDGDDK  PSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           RTCCSE NNDLDADLEI VEVPEENGEKEGTFSSDESRPLKKSKSLSS NFHSDQIDNGA
Sbjct: 241 RTCCSETNNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAP +SEVMND VLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFG 600
           VFSY DWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHFG
Sbjct: 541 VFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of PI0008652.1 vs. ExPASy TrEMBL
Match: A0A1S3BIS3 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC103490332 PE=3 SV=1)

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 667/698 (95.56%), Postives = 675/698 (96.70%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDS+T       AVS+ P+SHSADNGTH TPQQLVAKAIAPV  DFLRPPPLRSSSN 
Sbjct: 1   MEEDSLT-------AVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTA NDGDDK  PSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           RTCCSE NNDLDADLEI VEVPEENGEKEGTFSSDESRPLKKSKSLSS NFHSDQIDNGA
Sbjct: 241 RTCCSETNNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAP +SEVMND VLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFG 600
           VFSY DWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHFG
Sbjct: 541 VFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of PI0008652.1 vs. ExPASy TrEMBL
Match: A0A5A7U7Y0 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G00190 PE=3 SV=1)

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 667/699 (95.42%), Postives = 675/699 (96.57%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDS+T       AVS+ P+SHSADNGTH TPQQLVAKAIAPV  DFLRPPPLRSSSN 
Sbjct: 1   MEEDSLT-------AVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTA NDGDDK  PSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-G 300
           RTCCSE NNDLDADLEI VEVPEENGEKEGTFSSDESRPLKKSKSLSS NFHSDQIDN G
Sbjct: 241 RTCCSETNNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGG 300

Query: 301 ACMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLP 360
           ACMIDETIGEGHAP +SEVMND VLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLP
Sbjct: 301 ACMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLP 360

Query: 361 FRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVE 420
           FRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVE
Sbjct: 361 FRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVE 420

Query: 421 LIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTG 480
           LIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTG
Sbjct: 421 LIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTG 480

Query: 481 YYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNG 540
           YYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNG
Sbjct: 481 YYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNG 540

Query: 541 DVFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHF 600
           DVFSY DWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHF
Sbjct: 541 DVFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHF 600

Query: 601 GLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMS 660
           GLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMS
Sbjct: 601 GLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMS 660

Query: 661 SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 692

BLAST of PI0008652.1 vs. ExPASy TrEMBL
Match: A0A6J1HXQ8 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC111468431 PE=3 SV=1)

HSP 1 Score: 1281.9 bits (3316), Expect = 0.0e+00
Identity = 627/696 (90.09%), Postives = 662/696 (95.11%), Query Frame = 0

Query: 3   EDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNVK 62
           EDSV+A+L D  AVS++P S SA  G  LTP++LVAKAIAPVKR++LRPPP+RSS NNVK
Sbjct: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61

Query: 63  DATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122
           DA ST KNDGDDK APS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 62  DAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121

Query: 123 KCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
           KCRFSHDIEAFKSQKPADL+GECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181

Query: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAERT 242
           EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE+KE+AVSNDS VA+RT
Sbjct: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241

Query: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
           CCSE+NND+DADL+I +EVPEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 242 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 301

Query: 303 IDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
           ID TI EGH P +SEV+NDSVL DS+ASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 302 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361

Query: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421

Query: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 481

Query: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
           GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541

Query: 543 SYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLE 602
           SY +WN+HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHFGLE
Sbjct: 542 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601

Query: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661

Query: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 662 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of PI0008652.1 vs. NCBI nr
Match: XP_004148548.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_020245 [Cucumis sativus])

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 679/698 (97.28%), Postives = 689/698 (98.71%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDSVTADLLDD AVS+ P+SHS+DNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN
Sbjct: 1   MEEDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTAKNDGDDK APSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY
Sbjct: 61  LKDATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKED+ENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           R CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA
Sbjct: 241 RPCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAP +SE MNDSVLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFG 600
           VFSYTDWN+HK+ECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDF HFG
Sbjct: 541 VFSYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of PI0008652.1 vs. NCBI nr
Match: XP_008448025.1 (PREDICTED: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like isoform X1 [Cucumis melo] >TYK12191.1 tRNA-dihydrouridine(47) synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 667/698 (95.56%), Postives = 675/698 (96.70%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDS+T       AVS+ P+SHSADNGTH TPQQLVAKAIAPV  DFLRPPPLRSSSN 
Sbjct: 1   MEEDSLT-------AVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTA NDGDDK  PSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGA 300
           RTCCSE NNDLDADLEI VEVPEENGEKEGTFSSDESRPLKKSKSLSS NFHSDQIDNGA
Sbjct: 241 RTCCSETNNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGA 300

Query: 301 CMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360
           CMIDETIGEGHAP +SEVMND VLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF
Sbjct: 301 CMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPF 360

Query: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420
           RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL
Sbjct: 361 RRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVEL 420

Query: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480
           IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY
Sbjct: 421 IDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGY 480

Query: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540
           YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD
Sbjct: 481 YEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGD 540

Query: 541 VFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFG 600
           VFSY DWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHFG
Sbjct: 541 VFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFG 600

Query: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660
           LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS
Sbjct: 601 LEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSS 660

Query: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 DSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of PI0008652.1 vs. NCBI nr
Match: KAA0049681.1 (tRNA-dihydrouridine(47) synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 667/699 (95.42%), Postives = 675/699 (96.57%), Query Frame = 0

Query: 1   MEEDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNN 60
           MEEDS+T       AVS+ P+SHSADNGTH TPQQLVAKAIAPV  DFLRPPPLRSSSN 
Sbjct: 1   MEEDSLT-------AVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNT 60

Query: 61  VKDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRY 120
           +KDATSTA NDGDDK  PSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRY
Sbjct: 61  LKDATSTANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRY 120

Query: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180
           SDKCRFSHDIEAFKSQKPADLEGECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLKR
Sbjct: 121 SDKCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKR 180

Query: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAE 240
           CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVA+
Sbjct: 181 CSEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAD 240

Query: 241 RTCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-G 300
           RTCCSE NNDLDADLEI VEVPEENGEKEGTFSSDESRPLKKSKSLSS NFHSDQIDN G
Sbjct: 241 RTCCSETNNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGG 300

Query: 301 ACMIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLP 360
           ACMIDETIGEGHAP +SEVMND VLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLP
Sbjct: 301 ACMIDETIGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLP 360

Query: 361 FRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVE 420
           FRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVE
Sbjct: 361 FRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVE 420

Query: 421 LIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTG 480
           LIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTG
Sbjct: 421 LIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTG 480

Query: 481 YYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNG 540
           YYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNG
Sbjct: 481 YYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNG 540

Query: 541 DVFSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHF 600
           DVFSY DWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHF
Sbjct: 541 DVFSYADWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHF 600

Query: 601 GLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMS 660
           GLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMS
Sbjct: 601 GLEHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMS 660

Query: 661 SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 692

BLAST of PI0008652.1 vs. NCBI nr
Match: XP_038888663.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1332.0 bits (3446), Expect = 0.0e+00
Identity = 655/697 (93.97%), Postives = 682/697 (97.85%), Query Frame = 0

Query: 3   EDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLR-SSSNNV 62
           EDSVTA+LLDD AVS++PVSHSADNGT LTPQQLVAKAIAPVKR+FLRPPP+R SSSNNV
Sbjct: 2   EDSVTAELLDDTAVSEEPVSHSADNGTQLTPQQLVAKAIAPVKREFLRPPPVRSSSSNNV 61

Query: 63  KDATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYS 122
           KDATSTAK+D DDKPAPS LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYS
Sbjct: 62  KDATSTAKSDSDDKPAPSGLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYS 121

Query: 123 DKCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKRC 182
           DKCRFSHDIEAFKSQKPADL+GECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLK+C
Sbjct: 122 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 181

Query: 183 SEMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAER 242
           SEMNGLNKD+QKLLWKNKMKFPKADSKLKELGLL  GKSKLRVKEDKENAVSND VVA+R
Sbjct: 182 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLAQGKSKLRVKEDKENAVSNDIVVADR 241

Query: 243 TCCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGAC 302
           TCCS++NNDLDADLEISV+VPEEN ++EGTFSSDESRPLKKSKSLSSENFHSDQ+DNGA 
Sbjct: 242 TCCSKVNNDLDADLEISVKVPEENEQEEGTFSSDESRPLKKSKSLSSENFHSDQVDNGAR 301

Query: 303 MIDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR 362
           MIDETIGEGH P +SEVMNDSVLLDS+ASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR
Sbjct: 302 MIDETIGEGHTPIESEVMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFR 361

Query: 363 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 422
           RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI
Sbjct: 362 RVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELI 421

Query: 423 DKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYY 482
           +KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMK+VVEAASGIVDTPITIKVRTGY+
Sbjct: 422 NKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEAASGIVDTPITIKVRTGYF 481

Query: 483 EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDV 542
           EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAP+SLQVIGNGDV
Sbjct: 482 EGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPESLQVIGNGDV 541

Query: 543 FSYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGL 602
           FSY+DWN+H+DE PKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHFGL
Sbjct: 542 FSYSDWNRHRDEGPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGL 601

Query: 603 EHWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSD 662
           EHWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSD
Sbjct: 602 EHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSD 661

Query: 663 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 662 SAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of PI0008652.1 vs. NCBI nr
Match: XP_022969416.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 1281.9 bits (3316), Expect = 0.0e+00
Identity = 627/696 (90.09%), Postives = 662/696 (95.11%), Query Frame = 0

Query: 3   EDSVTADLLDDKAVSDQPVSHSADNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNVK 62
           EDSV+A+L D  AVS++P S SA  G  LTP++LVAKAIAPVKR++LRPPP+RSS NNVK
Sbjct: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61

Query: 63  DATSTAKNDGDDKPAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122
           DA ST KNDGDDK APS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 62  DAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121

Query: 123 KCRFSHDIEAFKSQKPADLEGECPFSGPEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
           KCRFSHDIEAFKSQKPADL+GECPFSG EQLCPYGLACRFAGTHKEGVPAGSSVTLK+CS
Sbjct: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKCS 181

Query: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVAERT 242
           EMNGLNKD+QKLLWKNKMKFPKAD+KLKELGLLGHGKSK RVKE+KE+AVSNDS VA+RT
Sbjct: 182 EMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDS-VADRT 241

Query: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
           CCSE+NND+DADL+I +EVPEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 242 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 301

Query: 303 IDETIGEGHAPNKSEVMNDSVLLDSEASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
           ID TI EGH P +SEV+NDSVL DS+ASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 302 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361

Query: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421

Query: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 481

Query: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
           GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541

Query: 543 SYTDWNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLE 602
           SY +WN+HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITS ERLNILKDFVHFGLE
Sbjct: 542 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601

Query: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661

Query: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 699
           AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 662 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of PI0008652.1 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 873.2 bits (2255), Expect = 1.4e-253
Identity = 437/677 (64.55%), Postives = 538/677 (79.47%), Query Frame = 0

Query: 32  TPQQLVAKAIAPVKRDFLRPPPLRSSSNNVKDATSTAKNDGDDKPAP-STLVKEKKSKRQ 91
           T ++L+ +++AP+K++FL PPP          + S  +ND  D  AP S LV+EKKSKRQ
Sbjct: 36  TSEELIERSMAPIKKEFLCPPP---------PSRSVKQNDAADVRAPQSGLVQEKKSKRQ 95

Query: 92  LKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLEGECPFSGP 151
           LKRERR++  ST +LCP++A+T DV SC+Y DKCRF+HDIEAFK+QK  D+EG+CPF   
Sbjct: 96  LKRERREQ--STINLCPQVARTEDVDSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVAS 155

Query: 152 EQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQKLLWKNKMKFPKADSKLK 211
              C YGL+CRF G+H++    G+S   K  SEMN  NK+ Q+LLWKNKM F  AD+KLK
Sbjct: 156 GMKCAYGLSCRFLGSHRD--ITGNSDD-KEKSEMNFFNKETQRLLWKNKMTFTNADAKLK 215

Query: 212 ELGLLGHGKSKLRVKE---DKENAVSNDSVVAERTCCSEMNNDLDADLEISVEVPEENGE 271
            LGLLGH K     +E   +K     N +   E    S ++++  +++   V++P     
Sbjct: 216 SLGLLGHAKKSNAAEEITAEKTQNGMNGTQATEVAVDSAVSSEHTSEMIQDVDIP----- 275

Query: 272 KEGTFSSDESRPLKKSKSLSSENFHSDQID---NGACMIDETIGEGHAPNKSEVMN-DSV 331
             G   ++E RP+KK+KS   +N  +  +    +G  + +ET   G+  +K+ V + DS+
Sbjct: 276 --GPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSI 335

Query: 332 -LLDSEASLKPHPRE-KKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTN 391
            ++++++SLK HPRE KKLIDFRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTN
Sbjct: 336 KIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTN 395

Query: 392 LLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIV 451
           LLQGQASEWALLRRHSSEDLFGVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+V
Sbjct: 396 LLQGQASEWALLRRHSSEDLFGVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMV 455

Query: 452 VNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASA 511
           VNK AGSALL KP+RMK +VE +S IV+TPITIKVRT ++EGKNRIDSLIADIGNWGA+A
Sbjct: 456 VNKSAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATA 515

Query: 512 VTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNQHKDECPKLATCM 571
           VTIHGR+RQQRYSK ADW+YI +C+  A  +LQVIGNGDV+SY DWN+HK +CP+L++CM
Sbjct: 516 VTIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCM 575

Query: 572 IARGALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLEHWGSDSKGVETTRHFLLEW 631
           IARGALIKPW+FTEIKEQR+WDITS ERLNI+KDFV FGL+HWGSD+KGVETTRHFLLEW
Sbjct: 576 IARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEW 635

Query: 632 LSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDGF 691
           LSYT RYIPVGLL+V+PQ++NWRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+GF
Sbjct: 636 LSYTFRYIPVGLLDVIPQQINWRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGF 691

Query: 692 SFAPKHKSNAYDRAENG 699
           +FAPKHKSNAYDRAENG
Sbjct: 696 TFAPKHKSNAYDRAENG 691

BLAST of PI0008652.1 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 107.5 bits (267), Expect = 4.5e-23
Identity = 83/309 (26.86%), Postives = 144/309 (46.60%), Query Frame = 0

Query: 341 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 400
           +D+++KL LAP+  VG L FR +    GAD+T GE      +  C   L   +     + 
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 401 RHSSEDLFGV----------QICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNK 460
           + +   +F            Q+  +      +A E++  +     IDINMGCP    +  
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVAT--IDINMGCPKAFSIQG 120

Query: 461 GAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIADIGNWGASAVTI 520
           G G+ALL+KP  +  ++      +D P+T K+R    +       L   I   G  A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180

Query: 521 HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNQHKDECPKLATCMIAR 580
           HGR    R    A W+ I     +A  S+ VI NGDV  Y D+++ K      A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIK-TATGAASVMVAR 240

Query: 581 GALIKPWLFTEIKEQRNWDITSAERLNILKDFVHFGLEHWGSDSKGVETTRHFLLEWLS- 633
           GA+    +F+  K + +W+       ++ K ++   +  W +D   V++T++ + E ++ 
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290

BLAST of PI0008652.1 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 2.9e-14
Identity = 58/204 (28.43%), Postives = 96/204 (47.06%), Query Frame = 0

Query: 344 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 403
           R K  +AP+     LPFR +C+  GA      M    + T   + +  E+   +      
Sbjct: 87  RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146

Query: 404 LFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTV 463
           LF VQ C   PDT+  A + ++  C  D++DIN+GCP  I      G+ L+     +K++
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206

Query: 464 VEAASGIVDTPITIKVRT-GYYEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSK--LA 523
           VE  +  ++ P++ K+R     E   +   ++ D    G S + +HGRTR ++  K   A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266

Query: 524 DWEYINRCSSLAPDSLQVIGNGDV 542
           DW  I    +     + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280