PI0006404.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0006404.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	chr02: 2547681 .. 2548715 (-)
Sequence length	1035
RNA-Seq Expression	PI0006404.1
Synteny	PI0006404.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGCATAATTCCCATGTGATTGCCCTCTTATTCATGGTAGCCATGTCATCCATTGTCGTCGGAGCACACGACCTTCTTCTGGGCGCATACTATGGACATGAGGGTAACAACCTCCCACCACCATGGAAGGTGGTGCAACTTTGTGAAGAATACAACATTCGTCGTATTCGGTTCAATGAACCAGATCTCAGTATTATCGAAGAGTTTCGTGGCACGGATATCGAAATCTCTTTTGGCGTACCAAACGGGTTGATAACAAACATGGCGACCAATCAATCGGTGGTGGAGGAGTGGTTTGTCAACTACATTGCACCATTCATCAACGATTTCACCATCAACTACATCGTTGTTGGTGACAAGGCCGTACCTGGATTGGACGGCAGCATATTGCCTGTCATGAAATCCCTTCAAGATCTCCTCAATGCCCGTTACCTCGGGCAAGTGAAAATCACTACATTGGTTGGCTTACCTGCTTTAGGGGTTCAAACCCCTCCGTCTAGTGGCGCCTTTGACCCAAACGTTGCTGAGAACATGAGAGGAATCCTTCAGTTCTTATGGAGACAAGGCTCGCCTTTGATGCTTAGCTTATATCCCTATGAGGCATATGCTTACACGGGATACAGTAATAATATAAGTTTGGGTTATGCAACATTCACCGCCCAATCAGAACAAACCCCCCCGACACGAAATGATGGGGACTTAAGTTATAACAACATCTTTGATGAATTGGTGGATGCTTTTTATTCCGCGATCGATAAGGCAAGTGTCGGAGATGTCGCCATTGCAATCGGGGAAACCGGGTGGCCGACCAATGGGAATTATGACGCGACACCAACACTTGCAGCTACATACAATATAAATTTTAAGAATCATATTACCTCTGGGAAGGGGACACCAAAGAAACAAAATATTTATATTGAAGGATTCATCCGAAGTTTATTCAATGAGAATGAAAAGCCTGAAGGGGAGTCACAATTTTATGGCATGTTCAATGTTGATTCCACACCTATTTATTCGCCTGTGTTTTAA

mRNA sequence

ATGGCAAAGCATAATTCCCATGTGATTGCCCTCTTATTCATGGTAGCCATGTCATCCATTGTCGTCGGAGCACACGACCTTCTTCTGGGCGCATACTATGGACATGAGGGTAACAACCTCCCACCACCATGGAAGGTGGTGCAACTTTGTGAAGAATACAACATTCGTCGTATTCGGTTCAATGAACCAGATCTCAGTATTATCGAAGAGTTTCGTGGCACGGATATCGAAATCTCTTTTGGCGTACCAAACGGGTTGATAACAAACATGGCGACCAATCAATCGGTGGTGGAGGAGTGGTTTGTCAACTACATTGCACCATTCATCAACGATTTCACCATCAACTACATCGTTGTTGGTGACAAGGCCGTACCTGGATTGGACGGCAGCATATTGCCTGTCATGAAATCCCTTCAAGATCTCCTCAATGCCCGTTACCTCGGGCAAGTGAAAATCACTACATTGGTTGGCTTACCTGCTTTAGGGGTTCAAACCCCTCCGTCTAGTGGCGCCTTTGACCCAAACGTTGCTGAGAACATGAGAGGAATCCTTCAGTTCTTATGGAGACAAGGCTCGCCTTTGATGCTTAGCTTATATCCCTATGAGGCATATGCTTACACGGGATACAGTAATAATATAAGTTTGGGTTATGCAACATTCACCGCCCAATCAGAACAAACCCCCCCGACACGAAATGATGGGGACTTAAGTTATAACAACATCTTTGATGAATTGGTGGATGCTTTTTATTCCGCGATCGATAAGGCAAGTGTCGGAGATGTCGCCATTGCAATCGGGGAAACCGGGTGGCCGACCAATGGGAATTATGACGCGACACCAACACTTGCAGCTACATACAATATAAATTTTAAGAATCATATTACCTCTGGGAAGGGGACACCAAAGAAACAAAATATTTATATTGAAGGATTCATCCGAAGTTTATTCAATGAGAATGAAAAGCCTGAAGGGGAGTCACAATTTTATGGCATGTTCAATGTTGATTCCACACCTATTTATTCGCCTGTGTTTTAA

Coding sequence (CDS)

ATGGCAAAGCATAATTCCCATGTGATTGCCCTCTTATTCATGGTAGCCATGTCATCCATTGTCGTCGGAGCACACGACCTTCTTCTGGGCGCATACTATGGACATGAGGGTAACAACCTCCCACCACCATGGAAGGTGGTGCAACTTTGTGAAGAATACAACATTCGTCGTATTCGGTTCAATGAACCAGATCTCAGTATTATCGAAGAGTTTCGTGGCACGGATATCGAAATCTCTTTTGGCGTACCAAACGGGTTGATAACAAACATGGCGACCAATCAATCGGTGGTGGAGGAGTGGTTTGTCAACTACATTGCACCATTCATCAACGATTTCACCATCAACTACATCGTTGTTGGTGACAAGGCCGTACCTGGATTGGACGGCAGCATATTGCCTGTCATGAAATCCCTTCAAGATCTCCTCAATGCCCGTTACCTCGGGCAAGTGAAAATCACTACATTGGTTGGCTTACCTGCTTTAGGGGTTCAAACCCCTCCGTCTAGTGGCGCCTTTGACCCAAACGTTGCTGAGAACATGAGAGGAATCCTTCAGTTCTTATGGAGACAAGGCTCGCCTTTGATGCTTAGCTTATATCCCTATGAGGCATATGCTTACACGGGATACAGTAATAATATAAGTTTGGGTTATGCAACATTCACCGCCCAATCAGAACAAACCCCCCCGACACGAAATGATGGGGACTTAAGTTATAACAACATCTTTGATGAATTGGTGGATGCTTTTTATTCCGCGATCGATAAGGCAAGTGTCGGAGATGTCGCCATTGCAATCGGGGAAACCGGGTGGCCGACCAATGGGAATTATGACGCGACACCAACACTTGCAGCTACATACAATATAAATTTTAAGAATCATATTACCTCTGGGAAGGGGACACCAAAGAAACAAAATATTTATATTGAAGGATTCATCCGAAGTTTATTCAATGAGAATGAAAAGCCTGAAGGGGAGTCACAATTTTATGGCATGTTCAATGTTGATTCCACACCTATTTATTCGCCTGTGTTTTAA

Protein sequence

MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF

Homology

[Close]

BLAST of PI0006404.1 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 7.2e-59
Identity = 123/317 (38.80%), Postives = 193/317 (60.88%), Query Frame = 0


Query: 29  LGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRG-TDIEISFGVPNGLI 88
           +G  YG  G+NLP P KV+ L +  +I +IR  +P+  ++   RG  DI ++ GV +  +
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 89  TNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDG-SILPVMKSLQDLLNARY 148
             ++ ++  V+ WF   I P+++D  I +I VG++ +PG  G  +LPVM+SL +L+ +R 
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 149 LGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAY 208
           L  + I+T+V +  L    PPS+G F     E +  +L+ L +  SP+++ +YPY  ++Y
Sbjct: 163 L-PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPY--FSY 222

Query: 209 TGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIG 268
               ++I L YATF  ++        DG L Y+N+FD + DAF  A++K  V D+ + + 
Sbjct: 223 ASDPSSIRLDYATFNTEAIVV----QDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVS 282

Query: 269 ETGWPTNGNYD-ATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEGE 328
           ETGWP+ GN +  TP +A TYN NF  HI SGKGTPK+ N  I+GF+ + FNEN+KP G 
Sbjct: 283 ETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGT 342

Query: 329 SQFYGMFNV-DSTPIYS 342
            Q +G++N  D  PIY+
Sbjct: 343 EQNFGLYNPNDMKPIYN 352

[Close]

BLAST of PI0006404.1 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 6.1e-58
Identity = 124/317 (39.12%), Postives = 190/317 (59.94%), Query Frame = 0


Query: 29  LGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRG-TDIEISFGVPNGLI 88
           +G  YG  G+NLP P  V+ L +   I RIR  +P+  ++   RG  DIE++ GV +  +
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 89  TNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDG-SILPVMKSLQDLLNARY 148
             +A ++  V+ WF   I  ++ D  I +I VG++ +PG  G  +LPVM+SL +L+ +R 
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 149 LGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAY 208
           L  + I+T+V +  L    PPS+G F     E +  +L+ L +  +P+++++YPY  +AY
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY--FAY 213

Query: 209 TGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIG 268
                NI L YA+F  +S        DG L Y+N+FD + DAF  A++K  V ++ + + 
Sbjct: 214 ASDPANIRLDYASFNTKSIVV----QDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVS 273

Query: 269 ETGWPT--NGNYDATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEG 328
           ETGWP+  NGN+  TP +A+TYN NF  HI SGKGTPK+ N  + GF+ + FNEN+KP G
Sbjct: 274 ETGWPSAGNGNF-TTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAG 333

Query: 329 ESQFYGMFN-VDSTPIY 341
             Q +G++N  D  PIY
Sbjct: 334 TEQNFGLYNPSDMKPIY 342

[Close]

BLAST of PI0006404.1 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 8.2e-55
Identity = 116/320 (36.25%), Postives = 185/320 (57.81%), Query Frame = 0


Query: 29  LGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRGTDIEISFGVPNGLIT 88
           +G  YG  GNNLPPP +VV L +  NI R+R  +P+ + ++  R ++I++   VP   + 
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 89  NMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDGS--ILPVMKSLQDLLNARY 148
           ++A+N S   +W    +  +    +  YI VG++ +PG D +  ILP M+++ + L++  
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 149 L-GQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYA 208
           L  Q+K++T V    LG   PPS+GAF       +  I+QFL   G+PL++++YPY  ++
Sbjct: 149 LQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPY--FS 208

Query: 209 YTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAI 268
           YTG    ISL YA FTA          DG  SY N+FD +VDA ++A+++    +VA+ +
Sbjct: 209 YTGNPGQISLPYALFTASGVVV----QDGRFSYQNLFDAIVDAVFAALERVGGANVAVVV 268

Query: 269 GETGWPT-NGNYDATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEG 328
            E+GWP+  G  +A+ + A TYN N   H+  G GTP++    IE +I  +FNEN+K  G
Sbjct: 269 SESGWPSAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGG 328

Query: 329 ESQFYGMFNVDSTPIYSPVF 345
             Q +G+F  +  P+Y   F
Sbjct: 329 IEQNFGLFYPNKQPVYQISF 340

[Close]

BLAST of PI0006404.1 vs. ExPASy Swiss-Prot
Match: P07979 (Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3)

HSP 1 Score: 207.2 bits (526), Expect = 2.9e-52
Identity = 118/338 (34.91%), Postives = 192/338 (56.80%), Query Frame = 0


Query: 9   IALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSII 68
           I LL ++  S+ +VGA    +G  YG  GNNLPP  +VVQL +  NIRR+R  +P+ + +
Sbjct: 15  IILLGLLVSSTEIVGAQS--VGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAAL 74

Query: 69  EEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVP--- 128
           +  RG++IE+  GVPN  + N+A N S    W    +  F       YI VG++  P   
Sbjct: 75  QALRGSNIEVMLGVPNSDLQNIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTG 134

Query: 129 --GLDGSILPVMKSLQDLLNARYL-GQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRG 188
              L   +LP M+++++ +++  L   +K+++ V +  +G   PPS G+F  +V   +  
Sbjct: 135 TSSLTRYLLPAMRNIRNAISSAGLQNNIKVSSSVDMTLIGNSFPPSQGSFRNDVRSFIDP 194

Query: 189 ILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIF 248
           I+ F+ R  SPL++++YPY  ++Y G   +ISL YA FTA +        DG L Y N+F
Sbjct: 195 IIGFVRRINSPLLVNIYPY--FSYAGNPRDISLPYALFTAPNVVV----QDGSLGYRNLF 254

Query: 249 DELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGTPK 308
           D + DA Y+A+ +A  G + I + E+GWP+ G + AT   AATY  N   H+   +G+P+
Sbjct: 255 DAMSDAVYAALSRAGGGSIEIVVSESGWPSAGAFAATTNNAATYYKNLIQHVK--RGSPR 314

Query: 309 KQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIY 341
           + N  IE ++ ++F+EN K     + +G+F+ +  P Y
Sbjct: 315 RPNKVIETYLFAMFDENNKNPELEKHFGLFSPNKQPKY 342

[Close]

BLAST of PI0006404.1 vs. ExPASy Swiss-Prot
Match: A7PQW3 (Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120 PE=1 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 117/332 (35.24%), Postives = 185/332 (55.72%), Query Frame = 0


Query: 8   VIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSI 67
           V+ LL  V MS+I +GA    +G  YG  GNNLP   +V+ L +   I  +R  +P+   
Sbjct: 11  VVLLLGFVIMSTITIGAQS--IGVCYGRNGNNLPSASQVINLYKSNGIGSMRIYDPNSDT 70

Query: 68  IEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGL 127
           ++  RG+DIE+   VPN  + ++A++ S    W  N +  + ++    YI VG++ +P  
Sbjct: 71  LQALRGSDIELILDVPNTDLQSLASDASAAATWVQNNVVNYASEVKFRYIAVGNEVLPTG 130

Query: 128 DGS-----ILPVMKSLQDLLNARYL-GQVKITTLVGLPALGVQTPPSSGAFDPNVAENMR 187
             +     +LP MK++Q  + +  L  Q+K++T      LG   PPS G+F  +V+  + 
Sbjct: 131 SNAQYAQYVLPAMKNVQSAITSAGLQDQIKVSTATFSAVLGKSYPPSEGSFSDDVSSFIN 190

Query: 188 GILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNI 247
            I+ FL   GSPL+ ++YPY  ++YTG + NI L YA FTA          DG   Y N+
Sbjct: 191 PIISFLAENGSPLLANIYPY--FSYTGDTQNIRLDYALFTASGVVV----QDGSYQYQNL 250

Query: 248 FDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGTP 307
           FD L+DA Y+A++KA   ++ I + E+GWP+ G   AT   A TY  N  NH+    GTP
Sbjct: 251 FDALLDALYAALEKAGGSNLKIVVSESGWPSEGGTAATVDNARTYYKNLINHVKG--GTP 310

Query: 308 KKQNIYIEGFIRSLFNENEKPEGESQ-FYGMF 333
           +K    IE ++ ++F+EN+K   E++  +G+F
Sbjct: 311 RKSGA-IETYLFAMFDENQKTGLETEKHFGLF 331

[Close]

BLAST of PI0006404.1 vs. ExPASy TrEMBL
Match: A0A5D3CFE1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001650 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.6e-162
Identity = 289/345 (83.77%), Postives = 311/345 (90.14%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHNSHVIALLFMVAMSSIVVGA+++LLGAYYG E NNLP PWKV+QLCE+YNIRRIR 
Sbjct: 1   MAKHNSHVIALLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRI 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           +EP+L +IE FRGT+IE+SFGV NGLITNMATN + VEEWF NYIAPFI+D TINYIVVG
Sbjct: 61  DEPNLDVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DKAVPGLD SILPVMKSLQDLLN R+LGQVKITTLVGL ALGV+ PPSSGAFDPNV ENM
Sbjct: 121 DKAVPGLDNSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           RGILQFLW QGSPLMLSL PYEAYAY    NNISLGYATFT Q EQ PP R DG+LSYNN
Sbjct: 181 RGILQFLWGQGSPLMLSLNPYEAYAY----NNISLGYATFTPQLEQNPPIRIDGNLSYNN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYD-ATPTLAATYNINFKNHITSGKG 300
           +FDE+VDAFY+AIDKASVG VAIAIGETGWPTNG  D A+P LAATYNINFKNHI+SGKG
Sbjct: 241 LFDEVVDAFYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKG 300

Query: 301 TPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           TP K NIYIEGFI+SLFNENEKPEGES+FYGMFNVDSTPIYSPVF
Sbjct: 301 TPMKPNIYIEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of PI0006404.1 vs. ExPASy TrEMBL
Match: A0A5A7UQE4 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001680 PE=3 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 6.8e-161
Identity = 287/345 (83.19%), Postives = 310/345 (89.86%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHNSHVIALLFMV MSSIV+GA+++LLGAYYG E NNLP PWKV+QLCE+YNIRRIR 
Sbjct: 1   MAKHNSHVIALLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRI 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           +EP+L +IE FRGT+IE+SFGV NGLITNMATN + VEEWF NYIAPFI+D TINYIVVG
Sbjct: 61  DEPNLDVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DKAVPGLD SILPVMKSLQDLLN R+LGQVKITTLVGL ALGV+ PPSSGAFDPNV ENM
Sbjct: 121 DKAVPGLDDSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           RGILQFLW QGSPLMLSL PYEAYAY    NNISLGYATFT Q EQ PP R DG+LSYNN
Sbjct: 181 RGILQFLWGQGSPLMLSLNPYEAYAY----NNISLGYATFTPQLEQNPPIRIDGNLSYNN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYD-ATPTLAATYNINFKNHITSGKG 300
           +FDE+VDAFY+AIDKASVG VAIAIGETGWPTNG  D A+P LAATYNINFKNHI+SGKG
Sbjct: 241 LFDEVVDAFYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKG 300

Query: 301 TPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           TP K NIYIEGFI+SLFNENEKPEGES+FYGMFNVDSTPIYSPVF
Sbjct: 301 TPMKPNIYIEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of PI0006404.1 vs. ExPASy TrEMBL
Match: A0A0A0LR03 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G416760 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 8.8e-161
Identity = 273/344 (79.36%), Postives = 307/344 (89.24%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHN H IALLFMVAMSSIV   +++ +GAYYG EGNNLPPPWKVVQLCE+YNIRRIRF
Sbjct: 1   MAKHNFHAIALLFMVAMSSIVARGNNIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           NEP+L I+E+FRGT+IE+SF VP  LI N ATNQ+ VEEWFV Y+ PFI +FTINYIVVG
Sbjct: 61  NEPNLDILEQFRGTNIEVSFSVPGELIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DK +PGLD +ILPVM+SLQDLLN+RYLGQVKITT+VGL ALGVQ+PPSSGAFDPNV ENM
Sbjct: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           +GIL+FLW QGSPLMLSLYPY+AYAYTGY+NNISLGYATFT+Q+EQ PP R DG+L Y N
Sbjct: 181 KGILKFLWGQGSPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRIDGNLYYKN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGT 300
           +FD++VDAF++AIDKA  GDVAIAIGETGWPT GNY A P+LA TYN+NFKNHITSGKGT
Sbjct: 241 VFDDMVDAFHAAIDKACAGDVAIAIGETGWPTEGNYGAGPSLALTYNLNFKNHITSGKGT 300

Query: 301 PKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           P K NIYIEGFIRSLFNENEKPEGESQFYGMF+VDSTPIY  VF
Sbjct: 301 PMKPNIYIEGFIRSLFNENEKPEGESQFYGMFHVDSTPIYPSVF 344

[Close]

BLAST of PI0006404.1 vs. ExPASy TrEMBL
Match: A0A5A7UIV1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001690 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 2.8e-159
Identity = 281/345 (81.45%), Postives = 305/345 (88.41%), Query Frame = 0


Query: 1   MAKHNSHVIALL-FMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIR 60
           MAKHNSHVIALL FMVAMSSIVV A+D+LLGAYYG EGNNLPPP KVVQLC++YNIRRIR
Sbjct: 1   MAKHNSHVIALLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIR 60

Query: 61  FNEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVV 120
           F+EP+  II+ FRG DIE+SF V   LITNMATN + VEEWFVNY+APFI DFTINYI+V
Sbjct: 61  FSEPNFDIIDAFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIV 120

Query: 121 GDKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAEN 180
           GDKA+PGLD +ILPVMKSLQ LLN RYLGQVKITTLVGL ALGVQTPPSSG FDPNV EN
Sbjct: 121 GDKAIPGLDDNILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFDPNVLEN 180

Query: 181 MRGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYN 240
           MRGILQFL  QGSPLMLSLYPY+ YAYTG SNNISLGYA F +Q E+ PP R  GDLSYN
Sbjct: 181 MRGILQFLHGQGSPLMLSLYPYQEYAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYN 240

Query: 241 NIFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKG 300
           N+FDE+VD FY+AIDKA+VGDV I IGETGWPTNGNY  +P+LAATYN NFKNHI+SGKG
Sbjct: 241 NLFDEMVDTFYAAIDKANVGDVPIVIGETGWPTNGNYGGSPSLAATYNRNFKNHISSGKG 300

Query: 301 TPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           TP K NIYIEGFIRSLFNENEKPEGES+FYGMFNVDSTPIYSPVF
Sbjct: 301 TPMKPNIYIEGFIRSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 345

[Close]

BLAST of PI0006404.1 vs. ExPASy TrEMBL
Match: A0A5D3CDU1 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001660 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.0e-158
Identity = 279/345 (80.87%), Postives = 304/345 (88.12%), Query Frame = 0


Query: 1   MAKHNSHVIALL-FMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIR 60
           MAKHNSHVIALL FMVAMSSIVV A+D+LLGAYYG EGNNLPPP KVVQLC++YNIRRIR
Sbjct: 1   MAKHNSHVIALLYFMVAMSSIVVRAYDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIR 60

Query: 61  FNEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVV 120
           F+EP+  II+ FRG DIE+SF V   LITNMATN + VEEWFVNY+APFI DFTINYI+V
Sbjct: 61  FSEPNFDIIDAFRGKDIELSFSVTGELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIV 120

Query: 121 GDKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAEN 180
           GDKA+PGLD +ILPVMKSLQ LLN RYLGQVKITTLVGL ALGVQTPPSSG FDPNV EN
Sbjct: 121 GDKAIPGLDDNILPVMKSLQVLLNGRYLGQVKITTLVGLAALGVQTPPSSGTFDPNVLEN 180

Query: 181 MRGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYN 240
           MRGILQFL  QGSPLMLSLYPY+ YAYTG SNNISLGYA F +Q E+ PP R  GDLSYN
Sbjct: 181 MRGILQFLHGQGSPLMLSLYPYQEYAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYN 240

Query: 241 NIFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKG 300
           N+FDE+VD FY+AI KA+VGDV I IGETGWPTNGNY  +P+LAATYN NFKNHI+SGKG
Sbjct: 241 NLFDEMVDTFYAAIGKANVGDVPIVIGETGWPTNGNYGGSPSLAATYNRNFKNHISSGKG 300

Query: 301 TPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           TP K NIYIEGFIRSLFNENEKPEGES+FYGMF+VDSTPIYSPVF
Sbjct: 301 TPMKPNIYIEGFIRSLFNENEKPEGESRFYGMFDVDSTPIYSPVF 345

[Close]

BLAST of PI0006404.1 vs. NCBI nr
Match: XP_031742611.1 (glucan endo-1,3-beta-glucosidase-like [Cucumis sativus] >XP_031742618.1 glucan endo-1,3-beta-glucosidase-like [Cucumis sativus] >KAE8653447.1 hypothetical protein Csa_007612 [Cucumis sativus] >KAE8653450.1 hypothetical protein Csa_007638 [Cucumis sativus])

HSP 1 Score: 591.3 bits (1523), Expect = 5.5e-165
Identity = 281/344 (81.69%), Postives = 313/344 (90.99%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHN H IALLFMVAMSSIVV  +++L+GAYYG EGNNLPPPWKVVQLCE+YNIRRIRF
Sbjct: 1   MAKHNFHAIALLFMVAMSSIVVRGNNILVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           NEP+L I+E+FRGT+IE+SF VP  LI+N ATN + VEEWFV Y+ PFI +FTINYIVVG
Sbjct: 61  NEPNLDILEQFRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DK +PGLD +ILPVM+SLQDLLN+RYLGQVKITT+VGL ALGVQ+PPSSGAFDPNV ENM
Sbjct: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           +GIL+FLW QGSPLMLSLYPY+AYAYTGY+NNISLGYATFT+Q+EQ PP R DGDLSYNN
Sbjct: 181 KGILKFLWGQGSPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGT 300
           IFDE+VDAF++AIDKA VGDVAIAIGETGWPTNGNY A P+LA TYN+NFKNHITSGKGT
Sbjct: 241 IFDEMVDAFHAAIDKACVGDVAIAIGETGWPTNGNYGAGPSLALTYNLNFKNHITSGKGT 300

Query: 301 PKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           P K NIYIEGFIRSLFNENEKPEGESQFYGMF+VDSTPIY  VF
Sbjct: 301 PMKPNIYIEGFIRSLFNENEKPEGESQFYGMFHVDSTPIYPSVF 344

[Close]

BLAST of PI0006404.1 vs. NCBI nr
Match: TYK10032.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 580.9 bits (1496), Expect = 7.4e-162
Identity = 289/345 (83.77%), Postives = 311/345 (90.14%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHNSHVIALLFMVAMSSIVVGA+++LLGAYYG E NNLP PWKV+QLCE+YNIRRIR 
Sbjct: 1   MAKHNSHVIALLFMVAMSSIVVGAYNILLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRI 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           +EP+L +IE FRGT+IE+SFGV NGLITNMATN + VEEWF NYIAPFI+D TINYIVVG
Sbjct: 61  DEPNLDVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DKAVPGLD SILPVMKSLQDLLN R+LGQVKITTLVGL ALGV+ PPSSGAFDPNV ENM
Sbjct: 121 DKAVPGLDNSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           RGILQFLW QGSPLMLSL PYEAYAY    NNISLGYATFT Q EQ PP R DG+LSYNN
Sbjct: 181 RGILQFLWGQGSPLMLSLNPYEAYAY----NNISLGYATFTPQLEQNPPIRIDGNLSYNN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYD-ATPTLAATYNINFKNHITSGKG 300
           +FDE+VDAFY+AIDKASVG VAIAIGETGWPTNG  D A+P LAATYNINFKNHI+SGKG
Sbjct: 241 LFDEVVDAFYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKG 300

Query: 301 TPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           TP K NIYIEGFI+SLFNENEKPEGES+FYGMFNVDSTPIYSPVF
Sbjct: 301 TPMKPNIYIEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of PI0006404.1 vs. NCBI nr
Match: KAA0055781.1 (lichenase-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-160
Identity = 287/345 (83.19%), Postives = 310/345 (89.86%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHNSHVIALLFMV MSSIV+GA+++LLGAYYG E NNLP PWKV+QLCE+YNIRRIR 
Sbjct: 1   MAKHNSHVIALLFMVDMSSIVLGAYNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRI 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           +EP+L +IE FRGT+IE+SFGV NGLITNMATN + VEEWF NYIAPFI+D TINYIVVG
Sbjct: 61  DEPNLDVIESFRGTNIEVSFGVSNGLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DKAVPGLD SILPVMKSLQDLLN R+LGQVKITTLVGL ALGV+ PPSSGAFDPNV ENM
Sbjct: 121 DKAVPGLDDSILPVMKSLQDLLNRRFLGQVKITTLVGLSALGVKAPPSSGAFDPNVFENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           RGILQFLW QGSPLMLSL PYEAYAY    NNISLGYATFT Q EQ PP R DG+LSYNN
Sbjct: 181 RGILQFLWGQGSPLMLSLNPYEAYAY----NNISLGYATFTPQLEQNPPIRIDGNLSYNN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYD-ATPTLAATYNINFKNHITSGKG 300
           +FDE+VDAFY+AIDKASVG VAIAIGETGWPTNG  D A+P LAATYNINFKNHI+SGKG
Sbjct: 241 LFDEVVDAFYAAIDKASVGHVAIAIGETGWPTNGGNDGASPKLAATYNINFKNHISSGKG 300

Query: 301 TPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           TP K NIYIEGFI+SLFNENEKPEGES+FYGMFNVDSTPIYSPVF
Sbjct: 301 TPMKPNIYIEGFIKSLFNENEKPEGESRFYGMFNVDSTPIYSPVF 341

[Close]

BLAST of PI0006404.1 vs. NCBI nr
Match: KAE8653445.1 (hypothetical protein Csa_006879 [Cucumis sativus])

HSP 1 Score: 575.1 bits (1481), Expect = 4.1e-160
Identity = 270/340 (79.41%), Postives = 307/340 (90.29%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MA+HNSHVI+LLFM A+SSIVV  +D+L+GA+YG E N+LPPPWKVVQLC++YNIRRIRF
Sbjct: 1   MARHNSHVISLLFMAAISSIVVRGNDILIGAFYGSESNDLPPPWKVVQLCQKYNIRRIRF 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           NEP+  I+E+FRGT IE+SF VP  LI+N ATN + VEEWFV Y+ PFI +FTINYIVVG
Sbjct: 61  NEPNFDILEQFRGTGIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DK +PGLD +ILPVM+SLQDLLN+RYLGQVKITT+VGL ALGVQ+PPSSGAFDPNV ENM
Sbjct: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQSPPSSGAFDPNVLENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           +GIL+FLW QGSPLMLSLYPY+AYAYTGY+NNISLGYATFT+Q+EQ PP R DGDLSYNN
Sbjct: 181 KGILKFLWGQGSPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGT 300
           IFDE+VDAFY+AIDKA+VGDVAIAIGETGWPT GNY A P+LA TYN+NFKNHITSGKGT
Sbjct: 241 IFDEMVDAFYAAIDKANVGDVAIAIGETGWPTEGNYGAGPSLALTYNLNFKNHITSGKGT 300

Query: 301 PKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIY 341
           P   NIYIEGFIRSLFNENEKPEGESQFYGMF++D TPIY
Sbjct: 301 PMMPNIYIEGFIRSLFNENEKPEGESQFYGMFHIDFTPIY 340

[Close]

BLAST of PI0006404.1 vs. NCBI nr
Match: KAE8652284.1 (hypothetical protein Csa_021876 [Cucumis sativus])

HSP 1 Score: 572.8 bits (1475), Expect = 2.0e-159
Identity = 275/344 (79.94%), Postives = 305/344 (88.66%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRF 60
           MAKHN H IALLFMVAMSSIV   +++ +GAYYG EGNNLPPPWKVVQLCE+YNIRRIRF
Sbjct: 1   MAKHNFHAIALLFMVAMSSIVARGNNIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRF 60

Query: 61  NEPDLSIIEEFRGTDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVG 120
           NEP+L I+E+FRGTDIE+SF VP  LI N ATNQ+ VEEWF  Y+ PFI +FTINYIVVG
Sbjct: 61  NEPNLDILEQFRGTDIEVSFSVPGELIINQATNQTAVEEWFDKYVVPFIGEFTINYIVVG 120

Query: 121 DKAVPGLDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENM 180
           DK +PGLD +ILPVM+SLQDLLN+RYLGQVKITT+VGL ALGVQTPPSSGAFDPNV ENM
Sbjct: 121 DKVIPGLDDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGVQTPPSSGAFDPNVLENM 180

Query: 181 RGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNN 240
           +GILQFLW QGSPLMLSLYPY+AYAYTGY+ NISLGYATFT+Q+EQ PP R DG+L Y N
Sbjct: 181 KGILQFLWGQGSPLMLSLYPYDAYAYTGYT-NISLGYATFTSQTEQNPPIRIDGNLYYKN 240

Query: 241 IFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYDATPTLAATYNINFKNHITSGKGT 300
           +FDE+VDAF++AIDKA  GDVAIAIGETGWPT GNY A P+LA TYN+NFKNHITSGKGT
Sbjct: 241 VFDEMVDAFHAAIDKACAGDVAIAIGETGWPTEGNYGAGPSLALTYNLNFKNHITSGKGT 300

Query: 301 PKKQNIYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIYSPVF 345
           P K NIYIEGFIRSLFNENEKPEGESQFYGMF+VDSTPIY  VF
Sbjct: 301 PMKPNIYIEGFIRSLFNENEKPEGESQFYGMFHVDSTPIYPSVF 343

[Close]

BLAST of PI0006404.1 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 229.2 bits (583), Expect = 5.1e-60
Identity = 123/317 (38.80%), Postives = 193/317 (60.88%), Query Frame = 0


Query: 29  LGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRG-TDIEISFGVPNGLI 88
           +G  YG  G+NLP P KV+ L +  +I +IR  +P+  ++   RG  DI ++ GV +  +
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 89  TNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDG-SILPVMKSLQDLLNARY 148
             ++ ++  V+ WF   I P+++D  I +I VG++ +PG  G  +LPVM+SL +L+ +R 
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 149 LGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAY 208
           L  + I+T+V +  L    PPS+G F     E +  +L+ L +  SP+++ +YPY  ++Y
Sbjct: 163 L-PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPY--FSY 222

Query: 209 TGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIG 268
               ++I L YATF  ++        DG L Y+N+FD + DAF  A++K  V D+ + + 
Sbjct: 223 ASDPSSIRLDYATFNTEAIVV----QDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVS 282

Query: 269 ETGWPTNGNYD-ATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEGE 328
           ETGWP+ GN +  TP +A TYN NF  HI SGKGTPK+ N  I+GF+ + FNEN+KP G 
Sbjct: 283 ETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGT 342

Query: 329 SQFYGMFNV-DSTPIYS 342
            Q +G++N  D  PIY+
Sbjct: 343 EQNFGLYNPNDMKPIYN 352

[Close]

BLAST of PI0006404.1 vs. TAIR 10
Match: AT5G20390.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 123/316 (38.92%), Postives = 185/316 (58.54%), Query Frame = 0


Query: 29  LGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRGT-DIEISFGVPNGLI 88
           +G  YG  G+NLPPP +V+ L +  ++  IR  +    ++  FRG  +I +   V N  +
Sbjct: 33  IGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRNIGVMVDVKNQDL 92

Query: 89  TNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDGS-ILPVMKSLQDLLNARY 148
             ++ ++  V  WFV  I P++ D  I +I VG++ +PG  GS +LPVMKSL +++ +R 
Sbjct: 93  EALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGEIGSYVLPVMKSLTNIVKSRS 152

Query: 149 LGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAY 208
           L  + I+T V +  LG   PPS+G F P   E +  +L+FL +  +P+++++YPY  +AY
Sbjct: 153 L-PILISTTVAMTNLGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPY--FAY 212

Query: 209 TGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIG 268
                NI L YA F            DG L Y N+FD + DAF  A++K  V D+ + + 
Sbjct: 213 AADPINIQLDYAIFNTNKVVV----QDGPLGYTNMFDVIFDAFVWAMEKEGVKDLPMVVT 272

Query: 269 ETGWPTNGNYD-ATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEGE 328
           ETGWP+ GN +  TP +A+ YN NF  H+ SGKGTPK+    I GF+ + FNEN+KP G 
Sbjct: 273 ETGWPSAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGISGFLFATFNENQKPAGT 332

Query: 329 SQFYGMFN-VDSTPIY 341
            Q +G++N  D  PIY
Sbjct: 333 EQNFGLYNPTDMKPIY 341

[Close]

BLAST of PI0006404.1 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 226.1 bits (575), Expect = 4.3e-59
Identity = 124/317 (39.12%), Postives = 190/317 (59.94%), Query Frame = 0


Query: 29  LGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSIIEEFRG-TDIEISFGVPNGLI 88
           +G  YG  G+NLP P  V+ L +   I RIR  +P+  ++   RG  DIE++ GV +  +
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 89  TNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGLDG-SILPVMKSLQDLLNARY 148
             +A ++  V+ WF   I  ++ D  I +I VG++ +PG  G  +LPVM+SL +L+ +R 
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 149 LGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAY 208
           L  + I+T+V +  L    PPS+G F     E +  +L+ L +  +P+++++YPY  +AY
Sbjct: 154 L-PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPY--FAY 213

Query: 209 TGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIG 268
                NI L YA+F  +S        DG L Y+N+FD + DAF  A++K  V ++ + + 
Sbjct: 214 ASDPANIRLDYASFNTKSIVV----QDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVS 273

Query: 269 ETGWPT--NGNYDATPTLAATYNINFKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEG 328
           ETGWP+  NGN+  TP +A+TYN NF  HI SGKGTPK+ N  + GF+ + FNEN+KP G
Sbjct: 274 ETGWPSAGNGNF-TTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAG 333

Query: 329 ESQFYGMFN-VDSTPIY 341
             Q +G++N  D  PIY
Sbjct: 334 TEQNFGLYNPSDMKPIY 342

[Close]

BLAST of PI0006404.1 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.9e-54
Identity = 123/335 (36.72%), Postives = 186/335 (55.52%), Query Frame = 0


Query: 9   IALLFMVAMSSIVVGAHDLLLGAYYGHEGNNLPPPWKVVQLCEEYNIRRIRFNEPDLSII 68
           I L F    S  V  A+   +G  YG  G+N P P  V+ L +   I +IR  +P   ++
Sbjct: 16  IMLTFNYNTSGFVAAANS--IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVL 75

Query: 69  EEFRG-TDIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDFTINYIVVGDKAVPGL 128
              RG  +IE++ GV +  +  ++ N+  V+ WF   I P++ D  I +I VG++ +PG 
Sbjct: 76  NALRGHRNIEVTVGVRDQDLGALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPGP 135

Query: 129 DG-SILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSGAFDPNVAENMRGILQF 188
            G  +LPVM+SL  L+ +  L  + I+T+V +  L    PPS+G F     E +  +L+ 
Sbjct: 136 IGPQVLPVMQSLTILVKSMNL-PISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKL 195

Query: 189 LWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPTRNDGDLSYNNIFDELV 248
           L +  +P+++++YPY  + Y     NI L YATF  ++        DG L Y+N+FD + 
Sbjct: 196 LSQTSTPILVNIYPY--FPYASDPTNIPLDYATFNTKATVV----QDGPLGYSNMFDAIF 255

Query: 249 DAFYSAIDKASVGDVAIAIGETGWPTNGNYD-ATPTLAATYNINFKNHITSGKGTPKKQN 308
           DAF  A++K  V D+ + + ETGWP+ GN +  TP +A TYN NF  HI SGKGTPK+ N
Sbjct: 256 DAFVWAMEKEGVKDLPMVVSETGWPSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPN 315

Query: 309 IYIEGFIRSLFNENEKPEGESQFYGMFNVDSTPIY 341
             ++GF+ + FNEN+KP G          D  PIY
Sbjct: 316 NGMDGFLFATFNENQKPAG---------TDMKPIY 332

[Close]

BLAST of PI0006404.1 vs. TAIR 10
Match: AT5G20560.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.1e-53
Identity = 118/344 (34.30%), Postives = 196/344 (56.98%), Query Frame = 0


Query: 1   MAKHNSHVIALLFMVAMSSIVVGAHDL-------LLGAYYGHEGNNLPPPWKVVQLCEEY 60
           M  ++ ++  LLF  + +++++  +++       ++G  YG  G+NLP P  V++  +  
Sbjct: 1   MEDYSKNLFLLLF--SCTALIISYYNVDSLSTASVVGLNYGLLGDNLPSPSNVIKFYKSQ 60

Query: 61  NIRRIRFNEPDLSIIEEFRGT-DIEISFGVPNGLITNMATNQSVVEEWFVNYIAPFINDF 120
           N+ +IR  EP+  ++   RG  DI ++ G+ N  +  +A N+  V+ WF   I P+I D 
Sbjct: 61  NVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNEDLEALAANKDAVKSWFSTNIDPYIADV 120

Query: 121 TINYIVVGDKAVPG--LDGSILPVMKSLQDLLNARYLGQVKITTLVGLPALGVQTPPSSG 180
            I +I VG++A+PG      +LPV++SL DL+ +R L Q+ I+T V   +L    PPS+G
Sbjct: 121 NITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKSRNL-QISISTTVTTTSLARLKPPSAG 180

Query: 181 AFDPNVAENMRGILQFLWRQGSPLMLSLYPYEAYAYTGYSNNISLGYATFTAQSEQTPPT 240
              P   + +  +L+ L +  +P+ +++YPY  Y +     N+ L YA F   ++Q    
Sbjct: 181 VLTPQARQQLVPVLRLLSQTSTPIFVNIYPY--YFHASDPKNVPLEYANF--NNDQI--V 240

Query: 241 RNDGDLSYNNIFDELVDAFYSAIDKASVGDVAIAIGETGWPTNGNYD-ATPTLAATYNIN 300
             DG L Y+N+FD + DAF  A++K  V  + + + ETGWP+ GN    TP L  TY  N
Sbjct: 241 VKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSETGWPSAGNGGMTTPALQYTYIGN 300

Query: 301 FKNHITSGKGTPKKQNIYIEGFIRSLFNENEKPEGESQFYGMFN 334
           F  H+ SGKGTPK+ N  I+ +I   +NEN+KP G  Q +G+++
Sbjct: 301 FVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIYQHFGLYD 335

The following BLAST results are available for this feature:

BLAST of PI0006404.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49353	7.2e-59	38.80	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49352	6.1e-58	39.12	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O22317	8.2e-55	36.25	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
P07979	2.9e-52	34.91	Lichenase OS=Nicotiana plumbaginifolia OX=4092 GN=GN1 PE=2 SV=3	[more]
A7PQW3	1.2e-50	35.24	Glucan endo-1,3-beta-glucosidase OS=Vitis vinifera OX=29760 GN=VIT_06s0061g00120...	[more]

BLAST of PI0006404.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CFE1	3.6e-162	83.77	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7UQE4	6.8e-161	83.19	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A0A0LR03	8.8e-161	79.36	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G416760 PE=3...	[more]
A0A5A7UIV1	2.8e-159	81.45	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5D3CDU1	7.0e-158	80.87	(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of PI0006404.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031742611.1	5.5e-165	81.69	glucan endo-1,3-beta-glucosidase-like [Cucumis sativus] >XP_031742618.1 glucan e...	[more]
TYK10032.1	7.4e-162	83.77	lichenase-2-like [Cucumis melo var. makuwa]	[more]
KAA0055781.1	1.4e-160	83.19	lichenase-2-like [Cucumis melo var. makuwa]	[more]
KAE8653445.1	4.1e-160	79.41	hypothetical protein Csa_006879 [Cucumis sativus]	[more]
KAE8652284.1	2.0e-159	79.94	hypothetical protein Csa_021876 [Cucumis sativus]	[more]

BLAST of PI0006404.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20340.1	5.1e-60	38.80	beta-1,3-glucanase 5	[more]
AT5G20390.1	1.5e-59	38.92	Glycosyl hydrolase superfamily protein	[more]
AT5G20330.1	4.3e-59	39.12	beta-1,3-glucanase 4	[more]
AT1G33220.1	1.9e-54	36.72	Glycosyl hydrolase superfamily protein	[more]
AT5G20560.1	2.1e-53	34.30	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 28..344 e-value: 5.1E-91 score: 306.9
None	No IPR available	PANTHER	PTHR32227:SF305	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATED	coord: 21..341
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 29..341 e-value: 2.7E-65 score: 221.0
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 261..274
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 21..341
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 29..341

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0006404	PI0006404	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0006404.1-exon	PI0006404.1-exon-chr02:2547681..2548715	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0006404.1-cds	PI0006404.1-cds-chr02:2547681..2548715	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0006404.1	PI0006404.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity