PI0005216.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0005216.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	calmodulin-like
Location	chr10: 3847489 .. 3848013 (+)
Sequence length	525
RNA-Seq Expression	PI0005216.1
Synteny	PI0005216.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATATCTCAAATCTTCAAGTTTCCATGGCGAAGTATGAAGCAAGTAAGTTGATGAAGAAGCATCAAAATAATGATGAAGGTATTCGACTTAAAGCTTTCAATGACATTCCTCGAGCTTCAACTCAAATCAACAATAATCAAAGAATGGTAACGCATATCCTAAAATCTAATAATGATGTTGCACACAACCTTACCTCAAATGCCTATGTGAAACCAAACAAGCAATTGGAAGCTGGGAAAGCTCAATTAACCGCCACAAGTGTTCTTGATATAAATGCGTCTAATATGCAATTTAGTCGAGAGCAGATAAAAGAAATTTTTCAGTATCATGATAGCGACAATGATGGTTTTCTCAACTTAAGGGAGGTAACAAAAGCTTTTGCTTTTCTTGGCACAATTCTTCCATTATACAAAGCCTACCATGGCATGGTATATGCTGATACTAATAAAGATGGTCTTATTAGCGAGGATGAACTTGAAAAACTCATTGATTATGCATATAAATTCATCAAGAAACGATGA

mRNA sequence

ATGTATATCTCAAATCTTCAAGTTTCCATGGCGAAGTATGAAGCAAGTAAGTTGATGAAGAAGCATCAAAATAATGATGAAGGTATTCGACTTAAAGCTTTCAATGACATTCCTCGAGCTTCAACTCAAATCAACAATAATCAAAGAATGGTAACGCATATCCTAAAATCTAATAATGATGTTGCACACAACCTTACCTCAAATGCCTATGTGAAACCAAACAAGCAATTGGAAGCTGGGAAAGCTCAATTAACCGCCACAAGTGTTCTTGATATAAATGCGTCTAATATGCAATTTAGTCGAGAGCAGATAAAAGAAATTTTTCAGTATCATGATAGCGACAATGATGGTTTTCTCAACTTAAGGGAGGTAACAAAAGCTTTTGCTTTTCTTGGCACAATTCTTCCATTATACAAAGCCTACCATGGCATGGTATATGCTGATACTAATAAAGATGGTCTTATTAGCGAGGATGAACTTGAAAAACTCATTGATTATGCATATAAATTCATCAAGAAACGATGA

Coding sequence (CDS)

ATGTATATCTCAAATCTTCAAGTTTCCATGGCGAAGTATGAAGCAAGTAAGTTGATGAAGAAGCATCAAAATAATGATGAAGGTATTCGACTTAAAGCTTTCAATGACATTCCTCGAGCTTCAACTCAAATCAACAATAATCAAAGAATGGTAACGCATATCCTAAAATCTAATAATGATGTTGCACACAACCTTACCTCAAATGCCTATGTGAAACCAAACAAGCAATTGGAAGCTGGGAAAGCTCAATTAACCGCCACAAGTGTTCTTGATATAAATGCGTCTAATATGCAATTTAGTCGAGAGCAGATAAAAGAAATTTTTCAGTATCATGATAGCGACAATGATGGTTTTCTCAACTTAAGGGAGGTAACAAAAGCTTTTGCTTTTCTTGGCACAATTCTTCCATTATACAAAGCCTACCATGGCATGGTATATGCTGATACTAATAAAGATGGTCTTATTAGCGAGGATGAACTTGAAAAACTCATTGATTATGCATATAAATTCATCAAGAAACGATGA

Protein sequence

MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNNDVAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR

Homology

[Close]

BLAST of PI0005216.1 vs. ExPASy TrEMBL
Match: A0A0A0KIY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.0e-72
Identity = 146/176 (82.95%), Postives = 156/176 (88.64%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHIL--KSN 60
           MYISNLQVS AKYEASKLMKKH+NNDEG+ LKAFN IP AST+INN Q M T I+   SN
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  NDVAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGF 120
           + VAH LTSNAY+ P+KQL AGKAQ+T TSVLDI ASNMQFSREQIKEIFQYHDSDNDGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           LNLREVTKAFAFLGTILPLYKAYHGMVYAD NKDGLIS+DELEKL+DYAYKF  KR
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of PI0005216.1 vs. ExPASy TrEMBL
Match: A0A0A0KLF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 4.6e-57
Identity = 120/174 (68.97%), Postives = 138/174 (79.31%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           M+I  LQ+S AKYEASKLMKK++ N EG+ L AFND  R STQIN N+ M T  LK N+D
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           VAH L+SNA +K N Q+  GKAQ+    V D  ASNMQFSREQIKEIFQY+DSD DGFLN
Sbjct: 88  VAHKLSSNAQLKSNNQIR-GKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLN 147

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           +REVTKAFA LG+I P  KAYHGMVYADTNKDGLI EDEL+KLI+YA KF+KK+
Sbjct: 148 IREVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of PI0005216.1 vs. ExPASy TrEMBL
Match: A0A0A0KMQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 8.2e-46
Identity = 103/174 (59.20%), Postives = 133/174 (76.44%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           MYIS LQ SMA YEAS L+K+++ N + +++KAF+D+ R+STQI N +  V  ILK ++ 
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRE-SVLKILKPSDG 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           VAH L S A+++P  Q   GK + + T VLD  +SNM  SRE+IKEIFQYHD++NDGFLN
Sbjct: 61  VAHKLRSEAHIQPT-QRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
             E+TKAFAFLG++ P YKA +GMVYAD N+DGLISE EL+KLIDYA K IKK+
Sbjct: 121 RMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of PI0005216.1 vs. ExPASy TrEMBL
Match: A0A5A7VI66 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G001550 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.0e-45
Identity = 100/174 (57.47%), Postives = 132/174 (75.86%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           MYIS LQ SMA YEA   +K ++ + + +++K FND+ R+STQI   Q +V  ILKS++D
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDV-RSSTQI-GYQGIVPKILKSSDD 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           VAH L+  A+++P +Q   GK + +    LDI +SNM FSR+QIKEIF+YHD++NDGFLN
Sbjct: 61  VAHKLSLEAHIQPTQQ-TGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           + E+TKAFAFLG++ P YKA +GMVYAD N DGLISE EL+KLIDYA K IKK+
Sbjct: 121 IVELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of PI0005216.1 vs. ExPASy TrEMBL
Match: A0A6J1EP72 (calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 1.5e-44
Identity = 101/174 (58.05%), Postives = 124/174 (71.26%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           M+I  LQ SMAKYEAS+++KK   N + ++LKAFN I RASTQ+ NNQ MV  IL+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQV-NNQSMVPEILRDNKN 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           V    +SN ++K                     ASNMQFSREQ+KEIF+YHDSD DGFLN
Sbjct: 61  VGPKFSSNTHLK---------------------ASNMQFSREQMKEIFRYHDSDKDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           +RE+TKAFAFLG+I+P  KA +GM YAD NKDGLISE EL+KL+DYA KFIKK+
Sbjct: 121 IRELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

[Close]

BLAST of PI0005216.1 vs. NCBI nr
Match: KAE8648235.1 (hypothetical protein Csa_018526 [Cucumis sativus])

HSP 1 Score: 231.1 bits (588), Expect = 7.3e-57
Identity = 122/150 (81.33%), Postives = 130/150 (86.67%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHIL--KSN 60
           MYISNLQVS AKYEASKLMKKH+NNDEG+ LKAFN IP AST+INN Q M T I+   SN
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  NDVAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGF 120
           + VAH LTSNAY+ P+KQL AGKAQ+T TSVLDI ASNMQFSREQIKEIFQYHDSDNDGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYAD 149
           LNLREVTKAFAFLGTILPLYKAYHGM   D
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMTDYD 150

[Close]

BLAST of PI0005216.1 vs. NCBI nr
Match: KAA0065465.1 (putative calcium-binding protein CML11 [Cucumis melo var. makuwa])

HSP 1 Score: 189.9 bits (481), Expect = 1.9e-44
Identity = 100/174 (57.47%), Postives = 132/174 (75.86%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           MYIS LQ SMA YEA   +K ++ + + +++K FND+ R+STQI   Q +V  ILKS++D
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDV-RSSTQI-GYQGIVPKILKSSDD 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           VAH L+  A+++P +Q   GK + +    LDI +SNM FSR+QIKEIF+YHD++NDGFLN
Sbjct: 61  VAHKLSLEAHIQPTQQ-TGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           + E+TKAFAFLG++ P YKA +GMVYAD N DGLISE EL+KLIDYA K IKK+
Sbjct: 121 IVELTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

[Close]

BLAST of PI0005216.1 vs. NCBI nr
Match: XP_022930012.1 (calmodulin-2/4-like [Cucurbita moschata])

HSP 1 Score: 189.1 bits (479), Expect = 3.2e-44
Identity = 101/174 (58.05%), Postives = 124/174 (71.26%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           M+I  LQ SMAKYEAS+++KK   N + ++LKAFN I RASTQ+ NNQ MV  IL+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKSTRNGDNVQLKAFNGIARASTQV-NNQSMVPEILRDNKN 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           V    +SN ++K                     ASNMQFSREQ+KEIF+YHDSD DGFLN
Sbjct: 61  VGPKFSSNTHLK---------------------ASNMQFSREQMKEIFRYHDSDKDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           +RE+TKAFAFLG+I+P  KA +GM YAD NKDGLISE EL+KL+DYA KFIKK+
Sbjct: 121 IRELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFIKKK 152

[Close]

BLAST of PI0005216.1 vs. NCBI nr
Match: XP_023530739.1 (calmodulin-2/4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 186.8 bits (473), Expect = 1.6e-43
Identity = 99/174 (56.90%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           M+I  LQ SMAKYEAS+++KK+  N + ++LKAFN I RASTQ+ NNQ MV  IL+ N +
Sbjct: 1   MFIPKLQASMAKYEASEMIKKNTRNGDNVQLKAFNGIARASTQV-NNQGMVPEILRDNKN 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           V    +SN ++K                     ASNMQFSRE++KEIF+YHDSD DGFLN
Sbjct: 61  VGPKFSSNTHLK---------------------ASNMQFSREKMKEIFRYHDSDKDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           +RE+TKAFAFLG+I+P  KA +GM YAD NKDGLISE EL+KL+DYA KF+KK+
Sbjct: 121 IRELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLVDYAQKFMKKK 152

[Close]

BLAST of PI0005216.1 vs. NCBI nr
Match: XP_022930007.1 (uncharacterized protein LOC111436449 [Cucurbita moschata])

HSP 1 Score: 184.5 bits (467), Expect = 7.8e-43
Identity = 100/174 (57.47%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 1   MYISNLQVSMAKYEASKLMKKHQNNDEGIRLKAFNDIPRASTQINNNQRMVTHILKSNND 60
           M+IS LQ SMAKYEAS+L+KK+  N + ++LK+F+DI R STQ+ NNQ  +  ILK N +
Sbjct: 1   MFISKLQASMAKYEASELIKKYPRNGDDVQLKSFSDIARDSTQL-NNQGKIPEILKDNKN 60

Query: 61  VAHNLTSNAYVKPNKQLEAGKAQLTATSVLDINASNMQFSREQIKEIFQYHDSDNDGFLN 120
           V   L+SNA+ K +K                     +QFSREQ+KEIF+YHDSD DGFLN
Sbjct: 61  VGLKLSSNAHPKTSK---------------------LQFSREQMKEIFKYHDSDKDGFLN 120

Query: 121 LREVTKAFAFLGTILPLYKAYHGMVYADTNKDGLISEDELEKLIDYAYKFIKKR 175
           LRE+TKAFAFLG+I+P  KA +GM YAD NKDGLISE EL+KLIDYA KF+KK+
Sbjct: 121 LRELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 152

The following BLAST results are available for this feature:

BLAST of PI0005216.1 vs.


Match Name	E-value	Identity	Description

BLAST of PI0005216.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIY4	1.0e-72	82.95	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0KLF3	4.6e-57	68.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1	[more]
A0A0A0KMQ1	8.2e-46	59.20	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1	[more]
A0A5A7VI66	9.0e-45	57.47	Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1EP72	1.5e-44	58.05	calmodulin-2/4-like OS=Cucurbita moschata OX=3662 GN=LOC111436455 PE=4 SV=1	[more]

BLAST of PI0005216.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648235.1	7.3e-57	81.33	hypothetical protein Csa_018526 [Cucumis sativus]	[more]
KAA0065465.1	1.9e-44	57.47	putative calcium-binding protein CML11 [Cucumis melo var. makuwa]	[more]
XP_022930012.1	3.2e-44	58.05	calmodulin-2/4-like [Cucurbita moschata]	[more]
XP_023530739.1	1.6e-43	56.90	calmodulin-2/4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022930007.1	7.8e-43	57.47	uncharacterized protein LOC111436449 [Cucurbita moschata]	[more]

BLAST of PI0005216.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00450	RECOVERIN	coord: 153..171 score: 31.98 coord: 107..128 score: 31.82
None	No IPR available	GENE3D	1.10.238.10		coord: 12..169 e-value: 4.0E-13 score: 51.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 101..163 e-value: 5.6E-9 score: 36.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 99..134 score: 12.114401
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 112..124
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 148..160
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 95..164

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0005216	PI0005216	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0005216.1-exon	PI0005216.1-exon-chr10:3847489..3848013	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0005216.1-cds	PI0005216.1-cds-chr10:3847489..3848013	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0005216.1	PI0005216.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding