PI0003163.1 (mRNA) Melon (PI 482460) v1

BLAST of PI0003163.1 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.1e-191
Identity = 326/508 (64.17%), Postives = 394/508 (77.56%), Query Frame = 0

Query: 2   DSPTSRGGGDSTTRIAARSSM----IESFKGCGLTGLR---IDKEELRKKLQMPQYLRFA 61
           ++P S G   +T  +AAR S       + +GCG   L    IDKEELR +L MP+YLR A
Sbjct: 3   ETPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIA 62

Query: 62  MRDSIRLQDPSAGENR--LPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRL 121
           MRD I+ +D +   +   LPG       A+A      D  P +PMVVFINP+SGGRHG +
Sbjct: 63  MRDCIKRKDSTEISDHLLLPG------GAAA------DMAPHAPMVVFINPKSGGRHGPV 122

Query: 122 LKERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDG 181
           LKERLQ L+ EEQVFDLT+VKPHEFVRYGLGCL+ LA  GD CA++ REK+R+MVAGGDG
Sbjct: 123 LKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDG 182

Query: 182 TVGWVLGCLLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERAT 241
           TVGWVLGCL EL K  +  +PPVGVIPLGTGNDLSR+F WGGSFPFAW+SA+KR+L RAT
Sbjct: 183 TVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRAT 242

Query: 242 TGQIRKLDSMNIVMQLACFSFYTIRRNVELPHCMKPT-EELALDESLEIEEALSEKASCH 301
            G I +LDS  IV+ +           V+ P+ +KPT EE ALD++L+ +  +  KA  +
Sbjct: 243 LGSIARLDSWKIVVSMPSGEV------VDPPYSLKPTIEETALDQALDADGDVPPKAKSY 302

Query: 302 EGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPG 361
           EGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGP+ NK+IYS YSC QGWF TPC ++P 
Sbjct: 303 EGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPA 362

Query: 362 LRGLKNILRMHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGF 421
           LRGL+NI+++H+KK NC++WE++ +P SVRS+V LNL NYGSGRHPWGNL P+Y+EKRGF
Sbjct: 363 LRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGF 422

Query: 422 VEAQVDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEP 481
           VEA  DDGL+E+FGLKQGWHAS VM E+ISAKHI QAAAIRFELRGG+WK+AFLQMDGEP
Sbjct: 423 VEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEP 482

Query: 482 WKQPMSNEFSTFVEIKRVPCQSLMISGE 500
           WKQPM +++STFVEIK+VP QSLMI+GE
Sbjct: 483 WKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of PI0003163.1 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 3.7e-189
Identity = 319/504 (63.29%), Postives = 385/504 (76.39%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIES--FKGCGLTGLR---IDKEELRKKLQMPQYLRFAM 60
           MDSP S+        +A+R S  +S   +GCGL  L    +DK ELR++L MP+YLR AM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKE 120
           RD I+ +D SA    +P     P  A A      D  P +PMVVFINP SGGRHG +LKE
Sbjct: 61  RDCIKRKDSSA----IPDHLLLPGGAVA------DMAPHAPMVVFINPNSGGRHGPVLKE 120

Query: 121 RLQMLIGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVG 180
           RLQ L+ EEQVFDLT+VKPHEFVRYGLGCLE +A  GD CAK+ R ++R+MVAGGDGTVG
Sbjct: 121 RLQQLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVG 180

Query: 181 WVLGCLLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQ 240
           WVLGCL EL K  +  +PPVGVIPLGTGNDLSR+FGWGGSFPFAW+SA+KR+L RA+ G 
Sbjct: 181 WVLGCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGP 240

Query: 241 IRKLDSMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVF 300
           + +LDS  I++ +           V+ P+ +KP EE  LD+ L+        A  +EGVF
Sbjct: 241 VARLDSWKILVSMPSGEV------VDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVF 300

Query: 301 YNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGL 360
           YNY SIGMDAQVAYGFHHLRN KPYLAQGPI+NK+IYS + C QGWF TPC +DPGLRGL
Sbjct: 301 YNYLSIGMDAQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGL 360

Query: 361 KNILRMHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQ 420
           +NI+++H+KKVNC+ WE++ +P +VRS+VALNL++YGSG HPWGNL P+Y+EKRGFVEA 
Sbjct: 361 RNIMKIHIKKVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAH 420

Query: 421 VDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQP 480
            DDGL+E+FG KQGWHAS VM ELISAKHI QAAA+RFELRGG+W+DAFLQMDGEPWKQP
Sbjct: 421 CDDGLIEIFGFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQP 480

Query: 481 MSNEFSTFVEIKRVPCQSLMISGE 500
           MS E+STFVEIK+VP QSLMI+ E
Sbjct: 481 MSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of PI0003163.1 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.2e-184
Identity = 311/505 (61.58%), Postives = 388/505 (76.83%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKG------CGLTGLRIDKEELRKKLQMPQYLRFA 60
           M+SP+   G   T R+  R S ++SF          L  +R+ K ELR+++ +PQYLR A
Sbjct: 1   MESPSI--GDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIA 60

Query: 61  MRDSIRLQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLK 120
           +RD I  +D S           D  S+ A   E+N   PE P++VF+NP+SGGR G L+K
Sbjct: 61  IRDCILRKDDSF----------DASSSVAPPLENNALTPEVPLMVFVNPKSGGRQGPLIK 120

Query: 121 ERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTV 180
           ERLQ LI EEQV+DLT+VKP+EF+RYGLGCLE  A  GD CAK++REKMR++VAGGDGTV
Sbjct: 121 ERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTV 180

Query: 181 GWVLGCLLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTG 240
           GWVLGCL EL  + R PVPPV ++PLGTGNDLSR+FGWGGSFPFAWKSAIKR+L RA+  
Sbjct: 181 GWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVA 240

Query: 241 QIRKLDSMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGV 300
            I +LDS NI++ +           V+ P+ +K T+E  +D++LEIE  +    + +EGV
Sbjct: 241 PISRLDSWNILITMPSGEI------VDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGV 300

Query: 301 FYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRG 360
           FYNYFSIGMDAQVAYGFHHLRNEKPYLA GPIANK+IYSGY C QGWF T C +DPGLRG
Sbjct: 301 FYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRG 360

Query: 361 LKNILRMHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEA 420
           LKNI+ +H+KK++ ++WE+V +P SVR++VALNL++YGSGR+PWGNL  +Y+EKRGFVEA
Sbjct: 361 LKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEA 420

Query: 421 QVDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQ 480
           Q DDGLLE+FGLKQGWHAS VM ELISAKHI QAAAIR E+RGG+WKDAF+QMDGEPWKQ
Sbjct: 421 QADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQ 480

Query: 481 PMSNEFSTFVEIKRVPCQSLMISGE 500
           PM+ ++STFV+IKRVP QSL++ G+
Sbjct: 481 PMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of PI0003163.1 vs. ExPASy Swiss-Prot
Match: Q9C5E5 (Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.8e-69
Identity = 146/415 (35.18%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 87  ESNDEPPESPMVVFINPRSGGRHGRLLKERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLE 146
           E +   P SP++VFIN +SGG+ G  L    + L+   QVFDL    P + +R     LE
Sbjct: 31  EESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLE 90

Query: 147 LLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGCLLELEKKERRPVPPVGVIPLGTGNDL 206
            L +  D  A+ +REK++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L
Sbjct: 91  RLKD--DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLSH---PPPIATVPLGTGNNL 150

Query: 207 SRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLDSMNIVMQL-------ACFSFYTIRRN 266
              FGWG   P   ++A++  LE+    ++ K+D+ +I+M++       +C     +   
Sbjct: 151 PFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPL--- 210

Query: 267 VELPHCMKPTEELALDESLEIEEALSEKASCH--EGVFYNYFSIGMDAQVAYGFHHLRNE 326
            ELPH +     ++  + L        K  CH   G F+NYFS+GMDAQ++Y FH  R  
Sbjct: 211 -ELPHSLHAFHRVSPTDEL-------NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKL 270

Query: 327 KPYLAQGPIANKLIYSGYSCGQGWFFTPCSS--DPGLRGLKNILRMHVKKVNCADWEQVL 386
            P   +  + N+  Y    C QGWF   C+S   P  R +  + ++ +   N   W+ + 
Sbjct: 271 HPEKFKNQLVNQSTYVKLGCTQGWF---CASLFHPASRNIAQLAKVKIATRN-GQWQDLH 330

Query: 387 IPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLV 446
           IP S+RS+V LNL ++  G +PWG   P     RG     VDDGL+EV G +  WH  ++
Sbjct: 331 IPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVL 390

Query: 447 MGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMS-NEFSTFVEIKRV 490
           +        + QA  IRFE   G     F++MDGEPWKQP+  ++ +  VEI  +
Sbjct: 391 LAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHL 425

BLAST of PI0003163.1 vs. ExPASy Swiss-Prot
Match: F4JKI3 (Diacylglycerol kinase 6 OS=Arabidopsis thaliana OX=3702 GN=DGK6 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.7e-67
Identity = 140/414 (33.82%), Postives = 221/414 (53.38%), Query Frame = 0

Query: 83  AFVPESNDEP---------PESPMVVFINPRSGGRHGRLLKERLQMLIGEEQVFDLTDVK 142
           ++V    DEP         PE+P++VFIN +SGG+ G  L    + L+ ++QVFDL    
Sbjct: 25  SYVLSPEDEPEAQISCTTAPENPILVFINSKSGGQLGAELILTYRTLLNDKQVFDLEVET 84

Query: 143 PHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGCLLELEKKERRPVP 202
           P + ++     LE L +  D+ A  +R+K++++VAGGDGT GW+LG + +L        P
Sbjct: 85  PDKVLQRIYLNLERLKD--DSLASKIRDKLKIIVAGGDGTAGWLLGVVSDLNLSN---PP 144

Query: 203 PVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLDSMNIVMQLACFSF 262
           P+  +PLGTGN+L   FGWG   P   +S+++  L +    +  K+D+  I+M++     
Sbjct: 145 PIATVPLGTGNNLPFAFGWGKKNPGTDRSSVESFLGKVINAKEMKIDNWKILMRMKHPKE 204

Query: 263 YTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFSIGMDAQVAYGFHH 322
            +    ++LPH        +L      ++   E    + G F+NYFS+GMDAQV+Y FH 
Sbjct: 205 GSCDITLKLPH--------SLPRIFPSDQENMEGYHTYRGGFWNYFSLGMDAQVSYAFHS 264

Query: 323 LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKVNCADWEQ 382
            R   P   +  + N+  Y   SC QGWFF      P  + +  + ++ +   N   W  
Sbjct: 265 QRKLHPERFKNQLVNQSTYLKLSCTQGWFFASL-FHPSSQNIAKLAKIQICDRN-GQWND 324

Query: 383 VLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHAS 442
           + IP S+RS+V LNL ++  G +PWG   P+    R      VDDGL+E+ G +  WH  
Sbjct: 325 LHIPQSIRSIVCLNLPSFSGGLNPWGTPNPKKQRDRSLTAPFVDDGLIEIVGFRNAWHGL 384

Query: 443 LVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPM-SNEFSTFVEI 487
           +++        + QA  +R E + G  K A++++DGEPWKQP+ SN+ +  VEI
Sbjct: 385 ILLSPNGHGTRLAQANRVRLEFKKGAAKHAYMRIDGEPWKQPLPSNDETVMVEI 423

BLAST of PI0003163.1 vs. ExPASy TrEMBL
Match: A0A1S3BRW0 (diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103493013 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.6e-275
Identity = 463/499 (92.79%), Postives = 479/499 (95.99%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDPSAGE+RLPGTR D +SAS FVPES +EPPESPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGLGCLELLAELGD CAKDVR+K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK+RRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++ +            NV+LPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS
Sbjct: 241 SWHVFLSTPS------GENVQLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR
Sbjct: 301 IGMDAQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 493

BLAST of PI0003163.1 vs. ExPASy TrEMBL
Match: A0A0A0LK87 (diacylglycerol kinase OS=Cucumis sativus OX=3659 GN=Csa_2G058110 PE=3 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 2.6e-270
Identity = 456/499 (91.38%), Postives = 475/499 (95.19%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGG DSTTRIA+RSSMIESFKGCGLTGLRIDKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGDDSTTRIASRSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP+AGE+RLPGTR D ISAS  VPE+ ++PP+SPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPTAGESRLPGTRLDSISASTSVPETTEQPPDSPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGLGCLELLAELGD CAKDVREKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK+RRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++ +            N +LPHCMKPTEELALDESLEIEEALSEKASC+EGVFYNYFS
Sbjct: 241 SWHVFLSTPS------GENGKLPHCMKPTEELALDESLEIEEALSEKASCYEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR
Sbjct: 301 IGMDAQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKD+FLQMDGEPWKQ MSNEF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDSFLQMDGEPWKQSMSNEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 493

BLAST of PI0003163.1 vs. ExPASy TrEMBL
Match: A0A5D3DLD8 (diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold717G00080 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.9e-269
Identity = 453/489 (92.64%), Postives = 469/489 (95.91%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDPSAGE+RLPGTR D +SAS FVPES +EPPESPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGLGCLELLAELGD CAKDVR+K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK+RRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++ +            NV+LPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS
Sbjct: 241 SWHVFLSTPS------GENVQLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR
Sbjct: 301 IGMDAQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480

Query: 481 STFVEIKRV 490
           STFVEIKRV
Sbjct: 481 STFVEIKRV 483

BLAST of PI0003163.1 vs. ExPASy TrEMBL
Match: A0A6J1EPG0 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111436187 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.5e-257
Identity = 434/499 (86.97%), Postives = 459/499 (91.98%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRI ARSSMIESFKGCGLTG+RIDKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP AGE+RLPG     IS S F  +++D PPESPMVVFINPRSGGRHGRLLKERLQML
Sbjct: 61  LQDPGAGESRLPGA--SSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGL CLELLA LGD CAK+ REKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK R PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTG+IR+LD
Sbjct: 181 LLELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S  +      F       N++LPHCMKPTE+ ALDESLEIEEAL EKA+CHEGVFYNYFS
Sbjct: 241 SWQV------FLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILR
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKK+NC+DWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGL
Sbjct: 361 MHVKKINCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++AFLQMDGEPWKQPM++EF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 491

BLAST of PI0003163.1 vs. ExPASy TrEMBL
Match: A0A6J1KDS8 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111492334 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 432/499 (86.57%), Postives = 459/499 (91.98%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRI ARSSMIESFKGCGLTG+RIDKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP AGE+RLPG     IS S F  +++D PPESPMVVFINP+SGGRHGRLLKERLQML
Sbjct: 61  LQDPGAGESRLPGA--SSISGSPFAQDTDDGPPESPMVVFINPQSGGRHGRLLKERLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFD+TDVKPHEFVRYGL CLELLA LGD CAK+ REKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDITDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK R PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTG+IR+LD
Sbjct: 181 LLELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S  +      F       N++LPHCMKPTE+ ALDESLEIEEAL EKA+CHEGVFYNYFS
Sbjct: 241 SWQV------FLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILR
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKK+NC+DWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGL
Sbjct: 361 MHVKKINCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++AFLQMDGEPWKQPM++EF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 491

BLAST of PI0003163.1 vs. NCBI nr
Match: XP_008451852.1 (PREDICTED: diacylglycerol kinase 7-like [Cucumis melo])

HSP 1 Score: 958.0 bits (2475), Expect = 3.2e-275
Identity = 463/499 (92.79%), Postives = 479/499 (95.99%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDPSAGE+RLPGTR D +SAS FVPES +EPPESPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGLGCLELLAELGD CAKDVR+K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK+RRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++ +            NV+LPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS
Sbjct: 241 SWHVFLSTPS------GENVQLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR
Sbjct: 301 IGMDAQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 493

BLAST of PI0003163.1 vs. NCBI nr
Match: XP_004150329.1 (diacylglycerol kinase 7 [Cucumis sativus] >KGN61127.1 hypothetical protein Csa_021281 [Cucumis sativus])

HSP 1 Score: 940.6 bits (2430), Expect = 5.3e-270
Identity = 456/499 (91.38%), Postives = 475/499 (95.19%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGG DSTTRIA+RSSMIESFKGCGLTGLRIDKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGDDSTTRIASRSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP+AGE+RLPGTR D ISAS  VPE+ ++PP+SPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPTAGESRLPGTRLDSISASTSVPETTEQPPDSPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGLGCLELLAELGD CAKDVREKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK+RRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++ +            N +LPHCMKPTEELALDESLEIEEALSEKASC+EGVFYNYFS
Sbjct: 241 SWHVFLSTPS------GENGKLPHCMKPTEELALDESLEIEEALSEKASCYEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR
Sbjct: 301 IGMDAQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKD+FLQMDGEPWKQ MSNEF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDSFLQMDGEPWKQSMSNEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 493

BLAST of PI0003163.1 vs. NCBI nr
Match: KAA0055616.1 (diacylglycerol kinase 7-like [Cucumis melo var. makuwa] >TYK24453.1 diacylglycerol kinase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 937.2 bits (2421), Expect = 5.9e-269
Identity = 453/489 (92.64%), Postives = 469/489 (95.91%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKE+LRKKLQMPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDPSAGE+RLPGTR D +SAS FVPES +EPPESPMVVFINPRSGGRHGRLLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGLGCLELLAELGD CAKDVR+K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK+RRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++ +            NV+LPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS
Sbjct: 241 SWHVFLSTPS------GENVQLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFH+LRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR
Sbjct: 301 IGMDAQVAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480

Query: 481 STFVEIKRV 490
           STFVEIKRV
Sbjct: 481 STFVEIKRV 483

BLAST of PI0003163.1 vs. NCBI nr
Match: XP_038890957.1 (diacylglycerol kinase 7-like [Benincasa hispida])

HSP 1 Score: 924.1 bits (2387), Expect = 5.2e-265
Identity = 451/499 (90.38%), Postives = 469/499 (93.99%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           M+SP+SRG GDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKL+MPQYLRFAMRDSIR
Sbjct: 1   MNSPSSRGEGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLRMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDPSAGE+RLPG   + IS SAFVPE++D PP SPMVVFINPRSGGRHGRLLKERLQML
Sbjct: 61  LQDPSAGESRLPGA--NSISGSAFVPENDDGPPASPMVVFINPRSGGRHGRLLKERLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQ+FDLTDVKPHEFV+YGL CLELLA LGD CAKDVREKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQIFDLTDVKPHEFVKYGLRCLELLAGLGDACAKDVREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKKER PVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTGQIRKLD
Sbjct: 181 LLELEKKERLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S ++++            NVELPHCMKPTEE ALDESLEIEEALSEKASCHEGVFYNYFS
Sbjct: 241 SWHVILSTPS------GENVELPHCMKPTEEHALDESLEIEEALSEKASCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILR
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPRLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKKVNC+DWEQ+LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL
Sbjct: 361 MHVKKVNCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMS+EF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSDEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIK+VP  SLMISGE
Sbjct: 481 STFVEIKKVPSHSLMISGE 491

BLAST of PI0003163.1 vs. NCBI nr
Match: XP_023546114.1 (diacylglycerol kinase 7-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.8 bits (2324), Expect = 1.0e-257
Identity = 433/499 (86.77%), Postives = 460/499 (92.18%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLQMPQYLRFAMRDSIR 60
           MDSPTSRGGGDSTTRI ARSSMIESFKGCGLTG+RIDKE+LRK+L+MPQYLRFAMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKERLQML 120
           LQDP AGE+RLPG     IS S F  +++D PPESPMVVFINPRSGGRHGRLLKERLQML
Sbjct: 61  LQDPGAGESRLPGA--SSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQML 120

Query: 121 IGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGC 180
           I EEQVFDLTDVKPHEFVRYGL CLELLA LGD CAK+ REKMRVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGC 180

Query: 181 LLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLD 240
           LLELEKK R P+PPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSL+RATTG+IR+LD
Sbjct: 181 LLELEKKGRLPLPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLD 240

Query: 241 SMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFS 300
           S  + +   C        N++LPHCMKPTE+ ALDESLEIEEAL EKA+CHEGVFYNYFS
Sbjct: 241 SWQVFLSSPC------GENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILR 360
           IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDP LRGLKNILR
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILR 360

Query: 361 MHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGL 420
           MHVKK+NC+DWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYMEKRGFVEA VDDGL
Sbjct: 361 MHVKKINCSDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGL 420

Query: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEF 480
           LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEW++AFLQMDGEPWKQPM++EF
Sbjct: 421 LEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEF 480

Query: 481 STFVEIKRVPCQSLMISGE 500
           STFVEIKRVPCQSLMISGE
Sbjct: 481 STFVEIKRVPCQSLMISGE 491

BLAST of PI0003163.1 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 668.7 bits (1724), Expect = 3.7e-192
Identity = 326/508 (64.17%), Postives = 394/508 (77.56%), Query Frame = 0

Query: 2   DSPTSRGGGDSTTRIAARSSM----IESFKGCGLTGLR---IDKEELRKKLQMPQYLRFA 61
           ++P S G   +T  +AAR S       + +GCG   L    IDKEELR +L MP+YLR A
Sbjct: 3   ETPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIA 62

Query: 62  MRDSIRLQDPSAGENR--LPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRL 121
           MRD I+ +D +   +   LPG       A+A      D  P +PMVVFINP+SGGRHG +
Sbjct: 63  MRDCIKRKDSTEISDHLLLPG------GAAA------DMAPHAPMVVFINPKSGGRHGPV 122

Query: 122 LKERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDG 181
           LKERLQ L+ EEQVFDLT+VKPHEFVRYGLGCL+ LA  GD CA++ REK+R+MVAGGDG
Sbjct: 123 LKERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDG 182

Query: 182 TVGWVLGCLLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERAT 241
           TVGWVLGCL EL K  +  +PPVGVIPLGTGNDLSR+F WGGSFPFAW+SA+KR+L RAT
Sbjct: 183 TVGWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRAT 242

Query: 242 TGQIRKLDSMNIVMQLACFSFYTIRRNVELPHCMKPT-EELALDESLEIEEALSEKASCH 301
            G I +LDS  IV+ +           V+ P+ +KPT EE ALD++L+ +  +  KA  +
Sbjct: 243 LGSIARLDSWKIVVSMPSGEV------VDPPYSLKPTIEETALDQALDADGDVPPKAKSY 302

Query: 302 EGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPG 361
           EGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGP+ NK+IYS YSC QGWF TPC ++P 
Sbjct: 303 EGVFYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPA 362

Query: 362 LRGLKNILRMHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGF 421
           LRGL+NI+++H+KK NC++WE++ +P SVRS+V LNL NYGSGRHPWGNL P+Y+EKRGF
Sbjct: 363 LRGLRNIMKIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGF 422

Query: 422 VEAQVDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEP 481
           VEA  DDGL+E+FGLKQGWHAS VM E+ISAKHI QAAAIRFELRGG+WK+AFLQMDGEP
Sbjct: 423 VEAHCDDGLIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEP 482

Query: 482 WKQPMSNEFSTFVEIKRVPCQSLMISGE 500
           WKQPM +++STFVEIK+VP QSLMI+GE
Sbjct: 483 WKQPMKSDYSTFVEIKKVPFQSLMINGE 492

BLAST of PI0003163.1 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 662.5 bits (1708), Expect = 2.6e-190
Identity = 319/504 (63.29%), Postives = 385/504 (76.39%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIES--FKGCGLTGLR---IDKEELRKKLQMPQYLRFAM 60
           MDSP S+        +A+R S  +S   +GCGL  L    +DK ELR++L MP+YLR AM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLKE 120
           RD I+ +D SA    +P     P  A A      D  P +PMVVFINP SGGRHG +LKE
Sbjct: 61  RDCIKRKDSSA----IPDHLLLPGGAVA------DMAPHAPMVVFINPNSGGRHGPVLKE 120

Query: 121 RLQMLIGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTVG 180
           RLQ L+ EEQVFDLT+VKPHEFVRYGLGCLE +A  GD CAK+ R ++R+MVAGGDGTVG
Sbjct: 121 RLQQLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVG 180

Query: 181 WVLGCLLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTGQ 240
           WVLGCL EL K  +  +PPVGVIPLGTGNDLSR+FGWGGSFPFAW+SA+KR+L RA+ G 
Sbjct: 181 WVLGCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGP 240

Query: 241 IRKLDSMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGVF 300
           + +LDS  I++ +           V+ P+ +KP EE  LD+ L+        A  +EGVF
Sbjct: 241 VARLDSWKILVSMPSGEV------VDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVF 300

Query: 301 YNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGL 360
           YNY SIGMDAQVAYGFHHLRN KPYLAQGPI+NK+IYS + C QGWF TPC +DPGLRGL
Sbjct: 301 YNYLSIGMDAQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGL 360

Query: 361 KNILRMHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQ 420
           +NI+++H+KKVNC+ WE++ +P +VRS+VALNL++YGSG HPWGNL P+Y+EKRGFVEA 
Sbjct: 361 RNIMKIHIKKVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAH 420

Query: 421 VDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQP 480
            DDGL+E+FG KQGWHAS VM ELISAKHI QAAA+RFELRGG+W+DAFLQMDGEPWKQP
Sbjct: 421 CDDGLIEIFGFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQP 480

Query: 481 MSNEFSTFVEIKRVPCQSLMISGE 500
           MS E+STFVEIK+VP QSLMI+ E
Sbjct: 481 MSTEYSTFVEIKKVPYQSLMINNE 488

BLAST of PI0003163.1 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 647.5 bits (1669), Expect = 8.7e-186
Identity = 311/505 (61.58%), Postives = 388/505 (76.83%), Query Frame = 0

Query: 1   MDSPTSRGGGDSTTRIAARSSMIESFKG------CGLTGLRIDKEELRKKLQMPQYLRFA 60
           M+SP+   G   T R+  R S ++SF          L  +R+ K ELR+++ +PQYLR A
Sbjct: 1   MESPSI--GDSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIA 60

Query: 61  MRDSIRLQDPSAGENRLPGTRPDPISASAFVPESNDEPPESPMVVFINPRSGGRHGRLLK 120
           +RD I  +D S           D  S+ A   E+N   PE P++VF+NP+SGGR G L+K
Sbjct: 61  IRDCILRKDDSF----------DASSSVAPPLENNALTPEVPLMVFVNPKSGGRQGPLIK 120

Query: 121 ERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLELLAELGDTCAKDVREKMRVMVAGGDGTV 180
           ERLQ LI EEQV+DLT+VKP+EF+RYGLGCLE  A  GD CAK++REKMR++VAGGDGTV
Sbjct: 121 ERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTV 180

Query: 181 GWVLGCLLELEKKERRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLERATTG 240
           GWVLGCL EL  + R PVPPV ++PLGTGNDLSR+FGWGGSFPFAWKSAIKR+L RA+  
Sbjct: 181 GWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVA 240

Query: 241 QIRKLDSMNIVMQLACFSFYTIRRNVELPHCMKPTEELALDESLEIEEALSEKASCHEGV 300
            I +LDS NI++ +           V+ P+ +K T+E  +D++LEIE  +    + +EGV
Sbjct: 241 PISRLDSWNILITMPSGEI------VDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGV 300

Query: 301 FYNYFSIGMDAQVAYGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRG 360
           FYNYFSIGMDAQVAYGFHHLRNEKPYLA GPIANK+IYSGY C QGWF T C +DPGLRG
Sbjct: 301 FYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRG 360

Query: 361 LKNILRMHVKKVNCADWEQVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEA 420
           LKNI+ +H+KK++ ++WE+V +P SVR++VALNL++YGSGR+PWGNL  +Y+EKRGFVEA
Sbjct: 361 LKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEA 420

Query: 421 QVDDGLLEVFGLKQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQ 480
           Q DDGLLE+FGLKQGWHAS VM ELISAKHI QAAAIR E+RGG+WKDAF+QMDGEPWKQ
Sbjct: 421 QADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQ 480

Query: 481 PMSNEFSTFVEIKRVPCQSLMISGE 500
           PM+ ++STFV+IKRVP QSL++ G+
Sbjct: 481 PMTRDYSTFVDIKRVPHQSLVVKGD 487

BLAST of PI0003163.1 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 263.8 bits (673), Expect = 2.7e-70
Identity = 146/415 (35.18%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 87  ESNDEPPESPMVVFINPRSGGRHGRLLKERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLE 146
           E +   P SP++VFIN +SGG+ G  L    + L+   QVFDL    P + +R     LE
Sbjct: 31  EESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLE 90

Query: 147 LLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGCLLELEKKERRPVPPVGVIPLGTGNDL 206
            L +  D  A+ +REK++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L
Sbjct: 91  RLKD--DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLSH---PPPIATVPLGTGNNL 150

Query: 207 SRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLDSMNIVMQL-------ACFSFYTIRRN 266
              FGWG   P   ++A++  LE+    ++ K+D+ +I+M++       +C     +   
Sbjct: 151 PFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPL--- 210

Query: 267 VELPHCMKPTEELALDESLEIEEALSEKASCH--EGVFYNYFSIGMDAQVAYGFHHLRNE 326
            ELPH +     ++  + L        K  CH   G F+NYFS+GMDAQ++Y FH  R  
Sbjct: 211 -ELPHSLHAFHRVSPTDEL-------NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKL 270

Query: 327 KPYLAQGPIANKLIYSGYSCGQGWFFTPCSS--DPGLRGLKNILRMHVKKVNCADWEQVL 386
            P   +  + N+  Y    C QGWF   C+S   P  R +  + ++ +   N   W+ + 
Sbjct: 271 HPEKFKNQLVNQSTYVKLGCTQGWF---CASLFHPASRNIAQLAKVKIATRN-GQWQDLH 330

Query: 387 IPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLV 446
           IP S+RS+V LNL ++  G +PWG   P     RG     VDDGL+EV G +  WH  ++
Sbjct: 331 IPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVL 390

Query: 447 MGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMS-NEFSTFVEIKRV 490
           +        + QA  IRFE   G     F++MDGEPWKQP+  ++ +  VEI  +
Sbjct: 391 LAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHL 425

BLAST of PI0003163.1 vs. TAIR 10
Match: AT2G20900.3 (diacylglycerol kinase 5 )

HSP 1 Score: 263.8 bits (673), Expect = 2.7e-70
Identity = 146/415 (35.18%), Postives = 221/415 (53.25%), Query Frame = 0

Query: 87  ESNDEPPESPMVVFINPRSGGRHGRLLKERLQMLIGEEQVFDLTDVKPHEFVRYGLGCLE 146
           E +   P SP++VFIN +SGG+ G  L    + L+   QVFDL    P + +R     LE
Sbjct: 31  EESRPTPASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLE 90

Query: 147 LLAELGDTCAKDVREKMRVMVAGGDGTVGWVLGCLLELEKKERRPVPPVGVIPLGTGNDL 206
            L +  D  A+ +REK++++VAGGDGT GW+LG + +L+       PP+  +PLGTGN+L
Sbjct: 91  RLKD--DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLSH---PPPIATVPLGTGNNL 150

Query: 207 SRTFGWGGSFPFAWKSAIKRSLERATTGQIRKLDSMNIVMQL-------ACFSFYTIRRN 266
              FGWG   P   ++A++  LE+    ++ K+D+ +I+M++       +C     +   
Sbjct: 151 PFAFGWGKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPL--- 210

Query: 267 VELPHCMKPTEELALDESLEIEEALSEKASCH--EGVFYNYFSIGMDAQVAYGFHHLRNE 326
            ELPH +     ++  + L        K  CH   G F+NYFS+GMDAQ++Y FH  R  
Sbjct: 211 -ELPHSLHAFHRVSPTDEL-------NKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKL 270

Query: 327 KPYLAQGPIANKLIYSGYSCGQGWFFTPCSS--DPGLRGLKNILRMHVKKVNCADWEQVL 386
            P   +  + N+  Y    C QGWF   C+S   P  R +  + ++ +   N   W+ + 
Sbjct: 271 HPEKFKNQLVNQSTYVKLGCTQGWF---CASLFHPASRNIAQLAKVKIATRN-GQWQDLH 330

Query: 387 IPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQGWHASLV 446
           IP S+RS+V LNL ++  G +PWG   P     RG     VDDGL+EV G +  WH  ++
Sbjct: 331 IPHSIRSIVCLNLPSFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVL 390

Query: 447 MGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMS-NEFSTFVEIKRV 490
           +        + QA  IRFE   G     F++MDGEPWKQP+  ++ +  VEI  +
Sbjct: 391 LAPNGHGTRLAQANRIRFEFHKGATDHTFMRMDGEPWKQPLPLDDETVMVEISHL 425