PI0002166.1 (mRNA) Melon (PI 482460) v1

Overview


Name	PI0002166.1
Type	mRNA
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	GrpE protein homolog
Location	chr01: 11621364 .. 11624521 (+)
Sequence length	429
RNA-Seq Expression	PI0002166.1
Synteny	PI0002166.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGATCAAGTAAAATCACTGGCACGGAAGGAAAAGCTTTTGAAGTCTAAGCACAATGAGATTGAGAAAATGCAAGATGAAGTAGGCTCTTATGCAGAAATGGAGAATGTTATCGACGTAACAAGATGTGAAGCAGAGAATTCAAAGAAGTTTGCCATTCAGGTTTTTTATTGTTGTTCTTGTAAAACCTCAAGCCATTTAAGAGTTGAAGTAAAGACATTCCAGAATGCATTCAAGGAAAAAAATCTCGTACAATCTCTAGGAAACTTAGGTGAGAATTGAGGAATCCCGGGCAAATTTGAGAGATCACGGACGGAATTGGAAAATAGAAGTAAAATTCCAAGCTAATCTTGGTGTTTTCCAACTGAGATGGAAGAAATAATGGTTTTGGTGCTATGCATTTTGGTTTATAATGCTAAGAGAAGGAGGAAATTAAGATTTTTAAGTGTAAGTATGCGTTCTGCTTTGGTAACGCGAGGAAATTGTAAGGACAGGAGAAATCTCAGGCAATTTGTGGAATCCCAGACAAAATTGAAAACAGAAGGTGAATCTCAAGGTCAAGTTCGACCGAGATTGAAGGCCAAAGAGAGGAAGTGTAAGCTCTAGGGCCAATTCGGGGTTGTGTTTGGCCGAGACTGGAGATAGAAAAGGATTTATTTGAGTTCTCGAAAAAAACTCGGACCTTTCCAATCGAGAATAGATAGATCTCTGGGCATTCTTGATCGAGATCAGTAGAATTTAAGTGATTCGGTGAGTTGGAATCCAGGTGTTTAAGCATGCAAAGACCTAAATTTTGGCAAGTAGGAGATATAAGCTCAAAATTAATAGGTGAAAGGTGACAAGGTTTTGCCGTTTGGTGTTACAAAATTTTCATGCAAGAAAATCTATATAAGAGCAAGTTTGGTTGAAGAGTTCTCACCATTCACACATACCATTTGGATACTTCCACTTGGAAAAAAAATTAGAGTTTGAGGTGTTAAGTTGCTAAAAGGAATAAAGAGTTCAAGTTGGGGGAGGAAGCTTCAAGTTTTATGAGTGACTTTTCAAAATGAAAAGAAAAGTTTTCTAAGTTCTTTCTTTAGAATTTAATTATGTTATGTTCATGTTATGCATAGTAGGTTTATGTGATTATGAATATATGTTTATGTTGAGTTTTGATTTTGTAAATGAGTTCAAGCATGTTTTTGAAAGATCAAGTTATAAGTTTAAAGTATGTTTTAAAGCTTTCAAGCATGTTTTATCCTAGACCCGAATGTTAAAGAGTACTACCTATAGGACCTATTATGCATATGTGGTTAACTTCCCTACTGGGGGAAGGCTGACGAGCCTACTGGTCTACAATCCATCAGGAAAGATCTACTTTGTATGCCTATGAGAAGGTCCATTTTTAAATGACGAGGTGTTTCGTCACCTAGTACGTTTACTGATGATGGGGTGATTTGAAGTCCAGTCGTCTTATGACGAGGTGCTAGTACGCCTGAGTTATGAATCTATATTTCAAGAAAGGGTTATGAAATGGTTTCAAATGTTTAAAGTTCAAAAGTATTATGTTTATTTCATGCTTTGCTCACTGCTGAAATGTGTTATGAAAGATAAAATATTTTTACTTGTTTGATTGTATTCCCCAAAAAGGTTTTCAAAAAGCGTCACTCACTAAGCATTCGGCTTACGATTTAAGTTTCCCCTTCCCCAAGTTAGCAAGGCGTTCTGTTGCATTTTGAAGGTTAAGGCCAGCAGTTGGAGGCTGTTCGAAGTTTATTGTTTTGGTTAAAGGATATTTGTATTTGTATCTTACTTTGTACCAAGTTATGTTCAATAAAGTTAATTCAGTTTGGATTCACCTCTGTCTTTGCATGTTTGGATTGAAGCTTTGGAAGTTTAAAATGATAAGTTTTAAGATTGGGCATTCTGATGTTCCTCTAGAATGTTGGAATTGCCCAAGTTGCGACCCATCTCGGGATGCATGTAGGAGTATCTCGGGATGGGACGTGATAGTTCTCCTAGTGTTTTAAACACAAGCTTATGTGTGGTAACATATGCTTGACATAATTTTGCAAAGAGTTTGCTTGATGTTGCTGACAACTTGGGAAGAGCTTCTTCTGTTGTTAAAGATAGTTTCTCAAAAATGTTGCTTAATCTGAGGATTCAAGTGGAGCCTTAACTTTTCTAAAAACCCTTCTAGAAGGGGTCGAAATGACAGACAAACAGCTTATAGAGGTAAAGCTTTCCATTTTGCATCGAATGGAATTGGCATGAGCTATTCAACATTTCATGAAGAAAACCACAAGTTTAGTTTGTTCTGACGCTTCAAACACTTTTTTCACTGGGTATTTAGGAAGTCCGGGGTAGAGAAATTTGACCCTACAAATGAGCCATTTGATCCGCATAACCATTATGCTGTATTTTAGATACTGGATGGGTCTAAACCAGAAGGCACTGTGGCTATTGTCCTCAAGGTATTAGTTCAATTTGTGATTCTATAAATACCTCTGGATCATATGCCAAGGAAAATTCATTGATCAAACTGGAAGGGGGAACTGTCTTTCAGTCTATGTATGCTATAAAAAATTCAGTAATGTGAAGTTTGGTCGTTAGTCCTCGGGGTTCATGCTTAGATGGAAACCCTTTGGTCTGATGGTGTCTTTGCTCTTCTTTGGAGGCTTTGGTTTGAAAGAAACTCAGGATTTTTCATGACGATAATCCAAGTAGCGATGTCTTTTAGGATCTAGTTTTCGTTTTGCTTCTACTTGGTCTTCCACCTCTAAATTCTTTTGTAATTATAGCCTAACTTGTCTTTACACCAGTTTTACTTCCTTTTTGTAACTTGGTGTGTGCGGATGTCTCATCTTCCCCACTTTTGTATTTCCTTTTGTAGATGAAAGTTAAGTTTCTAATAAAAAAATTGGTTGTTAGTAGGAGCAGTCATGTCAATATGTTATAACAGATATATTTGCTTCCAAATTTTTTAAGAACCCAAAACCAAGACCATTACTCTTTAGTTCAGGAGCTTGGTTTGATAGACATTGCATTGGTTGTAGAGATATATGATTTTCTCATGTTTTGTACAACAGTCTGGATATATGCTACACGATCAAGTTCTACGGCCAACCAAGGTTGGTGTGACTCAAAGTGTGGAAGATATTTCAACCAATTAA

mRNA sequence

ATGGATGATCAAGTAAAATCACTGGCACGGAAGGAAAAGCTTTTGAAGTCTAAGCACAATGAGATTGAGAAAATGCAAGATGAAGTAGGCTCTTATGCAGAAATGGAGAATGTTATCGACGTAACAAGATGTGAAGCAGAGAATTCAAAGAAGTTTGCCATTCAGAGTTTGCTTGATGTTGCTGACAACTTGGGAAGAGCTTCTTCTGTTGATTCAAGTGGAGCCTTAACTTTTCTAAAAACCCTTCTAGAAGGGGTCGAAATGACAGACAAACAGCTTATAGAGTCCGGGGTAGAGAAATTTGACCCTACAAATGAGCCATTTGATCCGCATAACCATTATGCTTCTGGATATATGCTACACGATCAAGTTCTACGGCCAACCAAGGTTGGTGTGACTCAAAGTGTGGAAGATATTTCAACCAATTAA

Coding sequence (CDS)

ATGGATGATCAAGTAAAATCACTGGCACGGAAGGAAAAGCTTTTGAAGTCTAAGCACAATGAGATTGAGAAAATGCAAGATGAAGTAGGCTCTTATGCAGAAATGGAGAATGTTATCGACGTAACAAGATGTGAAGCAGAGAATTCAAAGAAGTTTGCCATTCAGAGTTTGCTTGATGTTGCTGACAACTTGGGAAGAGCTTCTTCTGTTGATTCAAGTGGAGCCTTAACTTTTCTAAAAACCCTTCTAGAAGGGGTCGAAATGACAGACAAACAGCTTATAGAGTCCGGGGTAGAGAAATTTGACCCTACAAATGAGCCATTTGATCCGCATAACCATTATGCTTCTGGATATATGCTACACGATCAAGTTCTACGGCCAACCAAGGTTGGTGTGACTCAAAGTGTGGAAGATATTTCAACCAATTAA

Protein sequence

MDDQVKSLARKEKLLKSKHNEIEKMQDEVGSYAEMENVIDVTRCEAENSKKFAIQSLLDVADNLGRASSVDSSGALTFLKTLLEGVEMTDKQLIESGVEKFDPTNEPFDPHNHYASGYMLHDQVLRPTKVGVTQSVEDISTN

Homology

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BLAST of PI0002166.1 vs. ExPASy Swiss-Prot
Match: Q8LB47 (GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.3e-34
Identity = 92/177 (51.98%), Postives = 115/177 (64.97%), Query Frame = 0


Query: 2   DDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ----S 61
           DD VK +A KE+LL  K  EI++++D+V  +YAEMENV+D TR +AEN+KK+A+Q    S
Sbjct: 142 DDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAEMENVMDRTRRDAENTKKYAVQNFAKS 201

Query: 62  LLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVEK 121
           LLDVADNLGRASSV            DS+GA   LKTLLEGVEMT+KQL E     G+EK
Sbjct: 202 LLDVADNLGRASSVVKESFSKLDTSEDSAGAAPLLKTLLEGVEMTEKQLAEVFKKFGMEK 261

Query: 122 FDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVED 139
           +DP NEPFDP+ H A                   SGY L+D+V+RP +VGVTQ  E+
Sbjct: 262 YDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPAEVGVTQGGEN 318

[Close]

BLAST of PI0002166.1 vs. ExPASy Swiss-Prot
Match: Q9FLP3 (GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.9e-26
Identity = 81/177 (45.76%), Postives = 108/177 (61.02%), Query Frame = 0


Query: 2   DDQVKSLARKEKLLKSKHNEIEKMQDE-VGSYAEMENVIDVTRCEAENSKKFAIQ----S 61
           DD VK ++ KE LLK +  +I +M+D+ + +YAE +N++D T   AE++KKFA+Q    S
Sbjct: 117 DDLVKLVSEKEDLLKVQQKDIMEMKDKFLRTYAEQQNLMDRTNRNAESAKKFAVQNFATS 176

Query: 62  LLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVEK 121
           LLDVADNL RASSV            D +GA   LK LLEGVEMT+KQL E    +G+ K
Sbjct: 177 LLDVADNLERASSVVKESFSKIDTSKDLAGATPLLKNLLEGVEMTEKQLAEVFRKAGLVK 236

Query: 122 FDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVED 139
            DP NEPF+P+ H A                   SGY L+D+V+RP +VGVT +VE+
Sbjct: 237 EDPLNEPFNPNRHNAVFQVPDASKPKGTIAHVLKSGYSLYDRVIRPAEVGVTCAVEN 293

[Close]

BLAST of PI0002166.1 vs. ExPASy Swiss-Prot
Match: A4YJR1 (Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.3e-10
Identity = 46/133 (34.59%), Postives = 74/133 (55.64%), Query Frame = 0


Query: 33  AEMENVIDVTRCEAENSKKFAI----QSLLDVADNLGRA----SSVDSSGALTFLKTLLE 92
           AEMEN+   T  E  +++ + I    + +LD+ADNL RA     +   + A   LK+L+E
Sbjct: 56  AEMENLRKRTAREVADARMYGITGFARDVLDIADNLQRALDAVPAETRANADAGLKSLIE 115

Query: 93  GVEMTDKQLI----ESGVEKFDPTNEPFDPHNHYA-------------------SGYMLH 135
           GVE+T++ L+    ++GV+KFDPT + FDP+   A                   +G+M+ 
Sbjct: 116 GVELTERSLLNTLEKNGVKKFDPTGQKFDPNFQQAMYEVPDPSVPSGTVVQVVQAGFMIG 175

[Close]

BLAST of PI0002166.1 vs. ExPASy Swiss-Prot
Match: P38523 (GrpE protein homolog, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MGE1 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 3.3e-10
Identity = 50/169 (29.59%), Postives = 81/169 (47.93%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDE-VGSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           + ++   + + E  L +K  E  +++D  + S A+  N+  VT+ + + +K FA+Q    
Sbjct: 60  LTEEQSEIKKLESQLSAKTKEASELKDRLLRSVADFRNLQQVTKKDIQKAKDFALQKFAK 119

Query: 61  SLLDVADNLGRASSVDSSGALTFLK---TLLEGVEMT----DKQLIESGVEKFDPTNEPF 120
            LL+  DN G A +      L   K    L  GV MT    +  L + G+EK DP  EPF
Sbjct: 120 DLLESVDNFGHALNAFKEEDLQKSKEISDLYTGVRMTRDVFENTLRKHGIEKLDPLGEPF 179

Query: 121 DPHNHYAS-------------------GYMLHDQVLRPTKVGVTQSVED 139
           DP+ H A+                   G+ L+D+V+RP KVG+ +  E+
Sbjct: 180 DPNKHEATFELPQPDKEPGTVFHVQQLGFTLNDRVIRPAKVGIVKGEEN 228

[Close]

BLAST of PI0002166.1 vs. ExPASy Swiss-Prot
Match: Q8YEV0 (Protein GrpE OS=Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) OX=224914 GN=grpE PE=3 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-09
Identity = 51/169 (30.18%), Postives = 88/169 (52.07%), Query Frame = 0


Query: 2   DDQVKSLARKEKLLKSKHNEIEKMQDEVGSYAEMENVIDVTRCEAENSKKFAI----QSL 61
           + +V   A +  +L++ + E++     V   AEMEN+   T+ + ++++ +AI    + +
Sbjct: 48  EGEVDETANRIAVLEADNTELKDQMLRVA--AEMENLRKRTQRDVQDARAYAITNFARDM 107

Query: 62  LDVADNLGRASSVDSSGAL---TFLKTLLEGVEMTDKQLI----ESGVEKFDPTNEPFDP 121
           L V+DNL RA     + AL   + LK+L EGVEMT++ ++      GV+K +P  + FDP
Sbjct: 108 LSVSDNLRRALDAIPADALEADSNLKSLSEGVEMTERAMLLALERHGVKKLEPEGQKFDP 167

Query: 122 HNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIS 141
           + H A                   +GY + D+VLRP  VGV++    +S
Sbjct: 168 NFHQAMFEVPNPDLPNNTVVQVVQAGYAIGDRVLRPAMVGVSKGGPKVS 214

[Close]

BLAST of PI0002166.1 vs. ExPASy TrEMBL
Match: A0A6J1F7Z2 (GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111442938 PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.5e-45
Identity = 117/182 (64.29%), Postives = 131/182 (71.98%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSLARKE+LLKSKH EIEKMQ++V  SYAEMENV+D T+ EAENSKKFAIQ    
Sbjct: 143 MDDLVKSLARKEELLKSKHKEIEKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAK 202

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            D SGA+  LKTLLEGV+MT+KQL+E    SGVE
Sbjct: 203 SLLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVE 262

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIS 143
           KFDPT+EPFDPH HYA                   SGYMLHD+VLRP +VGVTQ+VED S
Sbjct: 263 KFDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNS 322

[Close]

BLAST of PI0002166.1 vs. ExPASy TrEMBL
Match: A0A6J1CCY7 (GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111010351 PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.7e-41
Identity = 111/177 (62.71%), Postives = 125/177 (70.62%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSLARKE+LLKSKH EI+KMQD+V  SYAEMENV D TR EAENSKKFAIQ    
Sbjct: 141 MDDLVKSLARKEELLKSKHKEIKKMQDKVLRSYAEMENVKDRTRREAENSKKFAIQNFAK 200

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            DSSGA+  LKTLLEGV+MT+KQL++    SGVE
Sbjct: 201 SLLDVADNLGRASSVVKDSFSKIKESEDSSGAVPLLKTLLEGVDMTEKQLVQVFKKSGVE 260

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVE 138
           KFDPT+EPFDPH H A                   SGYML+D+VLRP +VGVT++ E
Sbjct: 261 KFDPTSEPFDPHRHNAVFQISDGSKPDGTVAIVLKSGYMLYDRVLRPAEVGVTRAAE 317

[Close]

BLAST of PI0002166.1 vs. ExPASy TrEMBL
Match: A0A2P5F264 (GrpE nucleotide exchange factor OS=Trema orientale OX=63057 GN=TorRG33x02_122180 PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 3.2e-40
Identity = 108/178 (60.67%), Postives = 124/178 (69.66%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VK +A KE+LLKSKH EIEKMQD+V  SYAEMENV++ TR EAENSKKFAIQ    
Sbjct: 188 MDDLVKLVAEKEELLKSKHKEIEKMQDKVLRSYAEMENVMERTRREAENSKKFAIQNFAK 247

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            DS+GA+  LKTLLEGVEMT+KQLIE     GVE
Sbjct: 248 SLLDVADNLGRASSVVKESFSKIDTSKDSAGAVPLLKTLLEGVEMTEKQLIEVFKKYGVE 307

Query: 121 KFDPTNEPFDPHNHYAS-------------------GYMLHDQVLRPTKVGVTQSVED 139
           K+DP NEPFDPH H A+                   GYML+D+V+RP +VGVTQ+VE+
Sbjct: 308 KYDPINEPFDPHRHNAAFQIPDPSKPSGTVAVVLKPGYMLYDRVIRPAEVGVTQAVEN 365

[Close]

BLAST of PI0002166.1 vs. ExPASy TrEMBL
Match: A0A059BLJ8 (GrpE protein homolog OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F00651 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 5.5e-40
Identity = 106/180 (58.89%), Postives = 124/180 (68.89%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD  K +A KE+LLKSKH EIEKMQD+V  SYAEMENV+D TR +AENSKK+A+Q    
Sbjct: 135 MDDLAKLVAEKEELLKSKHKEIEKMQDKVLRSYAEMENVMDRTRRQAENSKKYAVQNFAK 194

Query: 61  SLLDVADNLGRASSV-----------DSSGALTFLKTLLEGVEMTDKQLIE----SGVEK 120
           SLLDVADNLGRASSV           DS GA   LKTLLEGV+MT+KQL+E     GVEK
Sbjct: 195 SLLDVADNLGRASSVVKESFSKIDAKDSDGAAPLLKTLLEGVDMTEKQLVEVFKKHGVEK 254

Query: 121 FDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIST 142
           +DPTNEPFDPH H A                   +GYML+D+VLRP +VGVTQ+VE+ +T
Sbjct: 255 YDPTNEPFDPHRHNAVFQMPDSSKPPGTVAVVLKAGYMLYDRVLRPAEVGVTQAVENDAT 314

[Close]

BLAST of PI0002166.1 vs. ExPASy TrEMBL
Match: A0A2P5AS62 (GrpE protein homolog OS=Parasponia andersonii OX=3476 GN=PanWU01x14_305620 PE=3 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.6e-39
Identity = 107/178 (60.11%), Postives = 123/178 (69.10%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VK +A KE+LLKSKH EIEKMQD+V  SYAEMENV++ TR EAENSKKFAIQ    
Sbjct: 154 MDDLVKLVAEKEELLKSKHKEIEKMQDKVLRSYAEMENVMERTRREAENSKKFAIQNFAK 213

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            DS+GA+  LKTLLEGVEMT+KQLIE     GVE
Sbjct: 214 SLLDVADNLGRASSVVKESFSKIDASKDSAGAVPLLKTLLEGVEMTEKQLIEVFKKYGVE 273

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVED 139
           K+DP NEPFDPH H A                    GY+L+D+V+RP +VGVTQ+VE+
Sbjct: 274 KYDPINEPFDPHRHSAVFQIPDPSKPSGTVAVVLKPGYVLYDRVIRPAEVGVTQAVEN 331

[Close]

BLAST of PI0002166.1 vs. NCBI nr
Match: XP_038898199.1 (grpE protein homolog 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 191.4 bits (485), Expect = 5.2e-45
Identity = 116/182 (63.74%), Postives = 131/182 (71.98%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSLARKE+LLKSKH E+EKMQD+V  SYAEMENV+D TR EAENSKKFAIQ    
Sbjct: 184 MDDLVKSLARKEELLKSKHKELEKMQDKVLRSYAEMENVMDRTRREAENSKKFAIQNFAK 243

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIES----GVE 120
           SLLDVADNLGRASSV            DSSGA+  LK+LLEGV+MT+KQL+E+    GVE
Sbjct: 244 SLLDVADNLGRASSVVKDSFSKINESQDSSGAVPLLKSLLEGVDMTEKQLMEAFTKFGVE 303

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIS 143
           KFDPT+EPFDPH H+A                   SGYMLHD+VLRP +VGVTQ+ ED S
Sbjct: 304 KFDPTSEPFDPHKHFAVFQIPDGSKPDGTVAIVLKSGYMLHDRVLRPAEVGVTQAAEDNS 363

[Close]

BLAST of PI0002166.1 vs. NCBI nr
Match: XP_022936267.1 (grpE protein homolog 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 191.4 bits (485), Expect = 5.2e-45
Identity = 117/182 (64.29%), Postives = 131/182 (71.98%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSLARKE+LLKSKH EIEKMQ++V  SYAEMENV+D T+ EAENSKKFAIQ    
Sbjct: 143 MDDLVKSLARKEELLKSKHKEIEKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAK 202

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            D SGA+  LKTLLEGV+MT+KQL+E    SGVE
Sbjct: 203 SLLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVE 262

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIS 143
           KFDPT+EPFDPH HYA                   SGYMLHD+VLRP +VGVTQ+VED S
Sbjct: 263 KFDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNS 322

[Close]

BLAST of PI0002166.1 vs. NCBI nr
Match: KAG6592058.1 (Transcriptional adapter ADA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 190.3 bits (482), Expect = 1.2e-44
Identity = 116/182 (63.74%), Postives = 131/182 (71.98%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSLARKE+LLKSKH EI+KMQ++V  SYAEMENV+D T+ EAENSKKFAIQ    
Sbjct: 729 MDDLVKSLARKEELLKSKHKEIDKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAK 788

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            D SGA+  LKTLLEGV+MT+KQL+E    SGVE
Sbjct: 789 SLLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVE 848

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIS 143
           KFDPT+EPFDPH HYA                   SGYMLHD+VLRP +VGVTQ+VED S
Sbjct: 849 KFDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNS 908

[Close]

BLAST of PI0002166.1 vs. NCBI nr
Match: KAG7024936.1 (grpE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 190.3 bits (482), Expect = 1.2e-44
Identity = 116/182 (63.74%), Postives = 131/182 (71.98%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSLARKE+LLKSKH EI+KMQ++V  SYAEMENV+D T+ EAENSKKFAIQ    
Sbjct: 145 MDDLVKSLARKEELLKSKHKEIDKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAK 204

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            D SGA+  LKTLLEGV+MT+KQL+E    SGVE
Sbjct: 205 SLLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVE 264

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVEDIS 143
           KFDPT+EPFDPH HYA                   SGYMLHD+VLRP +VGVTQ+VED S
Sbjct: 265 KFDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVEDNS 324

[Close]

BLAST of PI0002166.1 vs. NCBI nr
Match: XP_023536002.1 (grpE protein homolog 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 187.6 bits (475), Expect = 7.5e-44
Identity = 114/178 (64.04%), Postives = 127/178 (71.35%), Query Frame = 0


Query: 1   MDDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ---- 60
           MDD VKSL RKE+LLKSKH EIEKMQ++V  SYAEMENV+D T+ EAENSKKFAIQ    
Sbjct: 143 MDDLVKSLTRKEELLKSKHKEIEKMQEKVLCSYAEMENVMDRTKREAENSKKFAIQNFAK 202

Query: 61  SLLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVE 120
           SLLDVADNLGRASSV            D SGA+  LKTLLEGV+MT+KQL+E    SGVE
Sbjct: 203 SLLDVADNLGRASSVVKDSFSKIDESEDPSGAVALLKTLLEGVDMTEKQLMEVFKKSGVE 262

Query: 121 KFDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVED 139
           KFDPT+EPFDPH HYA                   SGYMLHD+VLRP +VGVTQ+VED
Sbjct: 263 KFDPTSEPFDPHKHYAVFQIPDGSKPDGTVAVVLKSGYMLHDRVLRPAEVGVTQAVED 320

[Close]

BLAST of PI0002166.1 vs. TAIR 10
Match: AT4G26780.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 145.6 bits (366), Expect = 3.1e-35
Identity = 92/177 (51.98%), Postives = 115/177 (64.97%), Query Frame = 0


Query: 2   DDQVKSLARKEKLLKSKHNEIEKMQDEV-GSYAEMENVIDVTRCEAENSKKFAIQ----S 61
           DD VK +A KE+LL  K  EI++++D+V  +YAEMENV+D TR +AEN+KK+A+Q    S
Sbjct: 142 DDLVKLVAEKEELLSEKEEEIKQLKDKVLRTYAEMENVMDRTRRDAENTKKYAVQNFAKS 201

Query: 62  LLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVEK 121
           LLDVADNLGRASSV            DS+GA   LKTLLEGVEMT+KQL E     G+EK
Sbjct: 202 LLDVADNLGRASSVVKESFSKLDTSEDSAGAAPLLKTLLEGVEMTEKQLAEVFKKFGMEK 261

Query: 122 FDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVED 139
           +DP NEPFDP+ H A                   SGY L+D+V+RP +VGVTQ  E+
Sbjct: 262 YDPINEPFDPNRHNAVFQVPDASKPEGTVAHVLKSGYTLYDRVIRPAEVGVTQGGEN 318

[Close]

BLAST of PI0002166.1 vs. TAIR 10
Match: AT5G55200.1 (Co-chaperone GrpE family protein )

HSP 1 Score: 120.2 bits (300), Expect = 1.4e-27
Identity = 81/177 (45.76%), Postives = 108/177 (61.02%), Query Frame = 0


Query: 2   DDQVKSLARKEKLLKSKHNEIEKMQDE-VGSYAEMENVIDVTRCEAENSKKFAIQ----S 61
           DD VK ++ KE LLK +  +I +M+D+ + +YAE +N++D T   AE++KKFA+Q    S
Sbjct: 117 DDLVKLVSEKEDLLKVQQKDIMEMKDKFLRTYAEQQNLMDRTNRNAESAKKFAVQNFATS 176

Query: 62  LLDVADNLGRASSV------------DSSGALTFLKTLLEGVEMTDKQLIE----SGVEK 121
           LLDVADNL RASSV            D +GA   LK LLEGVEMT+KQL E    +G+ K
Sbjct: 177 LLDVADNLERASSVVKESFSKIDTSKDLAGATPLLKNLLEGVEMTEKQLAEVFRKAGLVK 236

Query: 122 FDPTNEPFDPHNHYA-------------------SGYMLHDQVLRPTKVGVTQSVED 139
            DP NEPF+P+ H A                   SGY L+D+V+RP +VGVT +VE+
Sbjct: 237 EDPLNEPFNPNRHNAVFQVPDASKPKGTIAHVLKSGYSLYDRVIRPAEVGVTCAVEN 293

The following BLAST results are available for this feature:

BLAST of PI0002166.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LB47	4.3e-34	51.98	GrpE protein homolog 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge2 PE...	[more]
Q9FLP3	1.9e-26	45.76	GrpE protein homolog 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=Mge1 PE...	[more]
A4YJR1	3.3e-10	34.59	Protein GrpE OS=Bradyrhizobium sp. (strain ORS 278) OX=114615 GN=grpE PE=3 SV=1	[more]
P38523	3.3e-10	29.59	GrpE protein homolog, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204...	[more]
Q8YEV0	1.3e-09	30.18	Protein GrpE OS=Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10...	[more]

BLAST of PI0002166.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F7Z2	2.5e-45	64.29	GrpE protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111442938 PE=3 SV=1	[more]
A0A6J1CCY7	1.7e-41	62.71	GrpE protein homolog OS=Momordica charantia OX=3673 GN=LOC111010351 PE=3 SV=1	[more]
A0A2P5F264	3.2e-40	60.67	GrpE nucleotide exchange factor OS=Trema orientale OX=63057 GN=TorRG33x02_122180...	[more]
A0A059BLJ8	5.5e-40	58.89	GrpE protein homolog OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_F00651 PE=3 SV=1	[more]
A0A2P5AS62	1.6e-39	60.11	GrpE protein homolog OS=Parasponia andersonii OX=3476 GN=PanWU01x14_305620 PE=3 ...	[more]

BLAST of PI0002166.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898199.1	5.2e-45	63.74	grpE protein homolog 2, mitochondrial-like [Benincasa hispida]	[more]
XP_022936267.1	5.2e-45	64.29	grpE protein homolog 2, mitochondrial-like [Cucurbita moschata]	[more]
KAG6592058.1	1.2e-44	63.74	Transcriptional adapter ADA2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7024936.1	1.2e-44	63.74	grpE, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023536002.1	7.5e-44	64.04	grpE protein homolog 2, mitochondrial-like isoform X2 [Cucurbita pepo subsp. pep...	[more]

BLAST of PI0002166.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26780.1	3.1e-35	51.98	Co-chaperone GrpE family protein	[more]
AT5G55200.1	1.4e-27	45.76	Co-chaperone GrpE family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 8..28
None	No IPR available	PANTHER	PTHR21237:SF35	GRPE PROTEIN HOMOLOG	coord: 1..115
IPR000740	GrpE nucleotide exchange factor	PFAM	PF01025	GrpE	coord: 7..115 e-value: 1.7E-16 score: 60.4
IPR000740	GrpE nucleotide exchange factor	PANTHER	PTHR21237	GRPE PROTEIN	coord: 1..115
IPR013805	GrpE nucleotide exchange factor, coiled-coil	GENE3D	3.90.20.20		coord: 2..96 e-value: 3.6E-13 score: 51.3
IPR013805	GrpE nucleotide exchange factor, coiled-coil	SUPERFAMILY	58014	Coiled-coil domain of nucleotide exchange factor GrpE	coord: 12..95
IPR009012	GrpE nucleotide exchange factor, head	SUPERFAMILY	51064	Head domain of nucleotide exchange factor GrpE	coord: 98..134

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
PI0002166	PI0002166	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0002166.1-exon	PI0002166.1-exon-chr01:11621364..11621528	exon
PI0002166.1-exon	PI0002166.1-exon-chr01:11623426..11623470	exon
PI0002166.1-exon	PI0002166.1-exon-chr01:11623509..11623583	exon
PI0002166.1-exon	PI0002166.1-exon-chr01:11623706..11623765	exon
PI0002166.1-exon	PI0002166.1-exon-chr01:11624438..11624521	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
PI0002166.1-cds	PI0002166.1-cds-chr01:11621364..11621528	CDS
PI0002166.1-cds	PI0002166.1-cds-chr01:11623426..11623470	CDS
PI0002166.1-cds	PI0002166.1-cds-chr01:11623509..11623583	CDS
PI0002166.1-cds	PI0002166.1-cds-chr01:11623706..11623765	CDS
PI0002166.1-cds	PI0002166.1-cds-chr01:11624438..11624521	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
PI0002166.1	PI0002166.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
biological_process	GO:0030150	protein import into mitochondrial matrix
cellular_component	GO:0001405	PAM complex, Tim23 associated import motor
molecular_function	GO:0000774	adenyl-nucleotide exchange factor activity
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0051082	unfolded protein binding