Moc10g12910.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g12910.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Thiamine thiazole synthase, chloroplastic
Location	chr10: 9810190 .. 9811864 (+)
Sequence length	1071
RNA-Seq Expression	Moc10g12910.1
Synteny	Moc10g12910.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTCCATTGCCTCCACTCTCGCCACCAATCTCCACAAGCCCTCCTTCTTGGACTCCTCCTCCTCCTTCCATGGCGTCCCTCTCGCCCCTCCCCCCTCCCTCCGCCTCAAGTCCGCCGCCCCCGCCGCCTTCTCCATCTCCGCCTCCGCCTCTTACGATCTCAACCAGTTCAACTTCAGCCCCATCAAGGAGTCCATCGTCGCCCGCGAGATGACCCGCCGCTACATGACCGACATGATCACTTACGCCGACACCGACGTCATCGTCGTCGGCGCCGGCTCCGCTGGCCTCTCCTGCGCCTACGAGCTCAGTAAGAACCCCTCTGTTCGCGTCGCCATCATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCCTGGCTCGGCGGCCAGCTCTTCTCTGCCATGGTATTCATTTACTTCCCATTTTTACCTCTGCCTTTAATCTAATCTGATGCATAACGTCTCTTTTTTTTTCCTTTTCCGGCTTACCATGCTCTGTTTTTTTCTCTTTCAAAGCATCCCTTTTAATCCTGCATGCCATATATAGCCATGAAAATTTATTTACTGTGGCACCCATGATCTGTCTTGCCCCTCAATTTCATGTTTCTTTAGGTCTATTATAAACCAAGATTTCTTTTTTCCTTTTATAACTTGCCCCTCAAAATCTGTGGCCGTTTTGACTCGTCGCCAGATTTCATTTTTGGGGTGGATCCTCGTTTGATTATGCTTGTTTGAGCGGTATTATATTAATATTGATTGGATTTCATCTCAAAATTATTGACCATACCAAGAGTAGTCCACATCTGGATCTAAAAATGATGAAGTTGAAATGAGTGTGTAAACATAGGAATTGATTGAAATTGTAAAGGGGGTTTTTTTAAAAAATGTTTGTGGAACACGAACAGGTGGTGAGGAAGCCGGCGCATTACTTCTTGGACGAGCTGGGCGTGGAGTACGACGAGCAGGATAACTACGTGGTGATAAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTTCTGGCCCGCCCCAATGTCAAGCTGTTCAACGCCGTGGCCGCCGAGGACTTGATCGTCAAGGGCGGCAGAGTCGGCGGCGTCGTCACCAACTGGGCGCTGGTGTCCATGAACCACGACACCCAGTCCTGCATGGATCCCAATGTCATGGAGGCCAAGGTGGTGGTCAGCTCCTGCGGCCACGACGGGCCCTTCGGCGCCACCGGCGTCAAGCGCCTCAAGTCCATCGGCATGATCGACAGCGTCCCCGGCATGAAGGCTCTCGACATGAACACGGCGGAGGACGCCATTGTTAGGCTCACCCGAGAGGTCGTCCCCGGTATGATCGTCACCGGCATGGAGGTCGCCGAGATCGACGGAGCTCCAAGAATGGTAATAACAACTAACACCACTCTAAAGAAATCATATCTGCTACTATAATTTCAATTTTGTTAAATTTTGTGTCTAATCAGTCTGTTTGAAAATTGAAAATCCAGGGACCGACTTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCGCACTTGGCACTGAAGTCATTGGGGCTGGGGAACGCCATAGATGGAGAAAGAAAGAAAGTGGAAATGAAGGATGAAGAAGCAGAGCTGCTAATCGCGGCGGCGGAATCGCCGGAGGTTGCAGATGCTTGA

mRNA sequence

ATGGCTTCCTCCATTGCCTCCACTCTCGCCACCAATCTCCACAAGCCCTCCTTCTTGGACTCCTCCTCCTCCTTCCATGGCGTCCCTCTCGCCCCTCCCCCCTCCCTCCGCCTCAAGTCCGCCGCCCCCGCCGCCTTCTCCATCTCCGCCTCCGCCTCTTACGATCTCAACCAGTTCAACTTCAGCCCCATCAAGGAGTCCATCGTCGCCCGCGAGATGACCCGCCGCTACATGACCGACATGATCACTTACGCCGACACCGACGTCATCGTCGTCGGCGCCGGCTCCGCTGGCCTCTCCTGCGCCTACGAGCTCAGTAAGAACCCCTCTGTTCGCGTCGCCATCATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCCTGGCTCGGCGGCCAGCTCTTCTCTGCCATGGTGGTGAGGAAGCCGGCGCATTACTTCTTGGACGAGCTGGGCGTGGAGTACGACGAGCAGGATAACTACGTGGTGATAAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTTCTGGCCCGCCCCAATGTCAAGCTGTTCAACGCCGTGGCCGCCGAGGACTTGATCGTCAAGGGCGGCAGAGTCGGCGGCGTCGTCACCAACTGGGCGCTGGTGTCCATGAACCACGACACCCAGTCCTGCATGGATCCCAATGTCATGGAGGCCAAGGTGGTGGTCAGCTCCTGCGGCCACGACGGGCCCTTCGGCGCCACCGGCGTCAAGCGCCTCAAGTCCATCGGCATGATCGACAGCGTCCCCGGCATGAAGGCTCTCGACATGAACACGGCGGAGGACGCCATTGTTAGGCTCACCCGAGAGGTCGTCCCCGGTATGATCGTCACCGGCATGGAGGTCGCCGAGATCGACGGAGCTCCAAGAATGGGACCGACTTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCGCACTTGGCACTGAAGTCATTGGGGCTGGGGAACGCCATAGATGGAGAAAGAAAGAAAGTGGAAATGAAGGATGAAGAAGCAGAGCTGCTAATCGCGGCGGCGGAATCGCCGGAGGTTGCAGATGCTTGA

Coding sequence (CDS)

ATGGCTTCCTCCATTGCCTCCACTCTCGCCACCAATCTCCACAAGCCCTCCTTCTTGGACTCCTCCTCCTCCTTCCATGGCGTCCCTCTCGCCCCTCCCCCCTCCCTCCGCCTCAAGTCCGCCGCCCCCGCCGCCTTCTCCATCTCCGCCTCCGCCTCTTACGATCTCAACCAGTTCAACTTCAGCCCCATCAAGGAGTCCATCGTCGCCCGCGAGATGACCCGCCGCTACATGACCGACATGATCACTTACGCCGACACCGACGTCATCGTCGTCGGCGCCGGCTCCGCTGGCCTCTCCTGCGCCTACGAGCTCAGTAAGAACCCCTCTGTTCGCGTCGCCATCATTGAGCAGTCCGTCAGCCCCGGCGGCGGCGCCTGGCTCGGCGGCCAGCTCTTCTCTGCCATGGTGGTGAGGAAGCCGGCGCATTACTTCTTGGACGAGCTGGGCGTGGAGTACGACGAGCAGGATAACTACGTGGTGATAAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTTCTGGCCCGCCCCAATGTCAAGCTGTTCAACGCCGTGGCCGCCGAGGACTTGATCGTCAAGGGCGGCAGAGTCGGCGGCGTCGTCACCAACTGGGCGCTGGTGTCCATGAACCACGACACCCAGTCCTGCATGGATCCCAATGTCATGGAGGCCAAGGTGGTGGTCAGCTCCTGCGGCCACGACGGGCCCTTCGGCGCCACCGGCGTCAAGCGCCTCAAGTCCATCGGCATGATCGACAGCGTCCCCGGCATGAAGGCTCTCGACATGAACACGGCGGAGGACGCCATTGTTAGGCTCACCCGAGAGGTCGTCCCCGGTATGATCGTCACCGGCATGGAGGTCGCCGAGATCGACGGAGCTCCAAGAATGGGACCGACTTTCGGGGCGATGATGATATCAGGGCAGAAGGCGGCGCACTTGGCACTGAAGTCATTGGGGCTGGGGAACGCCATAGATGGAGAAAGAAAGAAAGTGGAAATGAAGGATGAAGAAGCAGAGCTGCTAATCGCGGCGGCGGAATCGCCGGAGGTTGCAGATGCTTGA

Protein sequence

MASSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASASYDLNQFNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVADA

Homology

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BLAST of Moc10g12910.1 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 586.6 bits (1511), Expect = 1.4e-163
Identity = 311/359 (86.63%), Postives = 327/359 (91.09%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASAS---YDLNQF 62
           +SIASTL T L  PS L   SSFHG PLA PPSLRLKS A  + +ISASAS   YDLNQF
Sbjct: 2   ASIASTLTTKLQIPSLL-HDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 61

Query: 63  NFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQS 122
            F+PI+ESIV+REMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSKNP++R+AIIEQS
Sbjct: 62  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 121

Query: 123 VSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLAR 182
           VSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQD+YVVIKHAALFTSTIMSKLLAR
Sbjct: 122 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 181

Query: 183 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 242
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 182 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 241

Query: 243 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM 302
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 242 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 301

Query: 303 GPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKD--EEAELLIAAAESPEVADA 357
           GPTFGAMMISGQKAAHLALKSLG  N+I GE +  E K   EE ELL+AA ESPEVADA
Sbjct: 302 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

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BLAST of Moc10g12910.1 vs. NCBI nr
Match: XP_004152916.1 (thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_011133 [Cucumis sativus])

HSP 1 Score: 585.1 bits (1507), Expect = 4.1e-163
Identity = 309/359 (86.07%), Postives = 328/359 (91.36%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASAS---YDLNQF 62
           +SIASTL T L KPS L   SSFHG PLA P SLRLKS A  + +ISASAS   YDLNQF
Sbjct: 2   ASIASTLTTKLQKPSLL-HDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 61

Query: 63  NFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQS 122
            F+PI+ESIV+REMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP++R+AIIEQS
Sbjct: 62  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 121

Query: 123 VSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLAR 182
           VSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQD+YVVIKHAALFTSTIMSKLLAR
Sbjct: 122 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 181

Query: 183 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 242
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 182 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 241

Query: 243 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM 302
           FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 242 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 301

Query: 303 GPTFGAMMISGQKAAHLALKSLGLGNAI--DGERKKVEMKDEEAELLIAAAESPEVADA 357
           GPTFGAMMISGQKAAHLALKSLG  N+I  +GE  + ++  EE ELL+AA ESPEVADA
Sbjct: 302 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

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BLAST of Moc10g12910.1 vs. NCBI nr
Match: KAG7031400.1 (Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584.3 bits (1505), Expect = 6.9e-163
Identity = 309/360 (85.83%), Postives = 325/360 (90.28%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSS---SSFHGVPLAPPPSLRLKSAAPAAFSISASAS----YD 62
           +S+ASTL T L  PS   SS   SSFHG PLAPPPSLRLK   PA+FSISASAS    YD
Sbjct: 2   ASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLK-PTPASFSISASASQQQPYD 61

Query: 63  LNQFNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAI 122
           LN F F+PIKESIV+REMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP +R+AI
Sbjct: 62  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 121

Query: 123 IEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSK 182
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQ++YVVIKHAALFTSTIMSK
Sbjct: 122 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 181

Query: 183 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 242
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 182 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 241

Query: 243 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 302
           HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 242 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 301

Query: 303 APRMGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVAD 356
           APRMGPTFGAMMISGQKAAHLALKSLGL N I+         DE A+L++AA ESPEVAD
Sbjct: 302 APRMGPTFGAMMISGQKAAHLALKSLGLANCIE---------DEAADLILAAGESPEVAD 351

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BLAST of Moc10g12910.1 vs. NCBI nr
Match: XP_038905461.1 (thiamine thiazole synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 583.9 bits (1504), Expect = 9.1e-163
Identity = 314/365 (86.03%), Postives = 329/365 (90.14%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKS----AAPAAFSISASAS---YD 62
           +SIASTL T L +PS L   SSF+G PLAPPPSLRLKS     A  + SISASAS   YD
Sbjct: 2   ASIASTLTTKLQRPSLL-LDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYD 61

Query: 63  LNQFNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAI 122
           LNQF F+PIKESIV+REMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSKNPS+R+AI
Sbjct: 62  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 121

Query: 123 IEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSK 182
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQ++YVVIKHAALFTSTIMSK
Sbjct: 122 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 181

Query: 183 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 242
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 182 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 241

Query: 243 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 302
           HDGPFGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 242 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 301

Query: 303 APRMGPTFGAMMISGQKAAHLALKSLGLGNAID----GERKKVEMKDEEAELLIAAAESP 357
           APRMGPTFGAMMISGQKAAHLALKSLG  N I+    GERK V    EE ELL+AA ESP
Sbjct: 302 APRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVV----EEPELLLAAGESP 361

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BLAST of Moc10g12910.1 vs. NCBI nr
Match: KAG6600763.1 (Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 581.6 bits (1498), Expect = 4.5e-162
Identity = 308/361 (85.32%), Postives = 324/361 (89.75%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSS----SSFHGVPLAPPPSLRLKSAAPAAFSISASAS----Y 62
           +S+ASTL   L  PS   SS    SSFHG PLAPPPSLRLK   PA+FSISASAS    Y
Sbjct: 2   ASMASTLTIKLQTPSSSSSSSLLDSSFHGTPLAPPPSLRLK-PTPASFSISASASQQQPY 61

Query: 63  DLNQFNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVA 122
           DLN F F+PIKESIV+REMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP +R+A
Sbjct: 62  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIA 121

Query: 123 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMS 182
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQ++YVVIKHAALFTSTIMS
Sbjct: 122 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMS 181

Query: 183 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 242
           KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 182 KLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 241

Query: 243 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 302
           GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID
Sbjct: 242 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 301

Query: 303 GAPRMGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVA 356
           GAPRMGPTFGAMMISGQKAAHLALKSLGL N I+         DE A+L++AA ESPEVA
Sbjct: 302 GAPRMGPTFGAMMISGQKAAHLALKSLGLANCIE---------DEAADLILAAGESPEVA 352

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BLAST of Moc10g12910.1 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 6.1e-154
Identity = 291/361 (80.61%), Postives = 315/361 (87.26%), Query Frame = 0


Query: 1   MASSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRL---KSAAPAAFSISASAS--YD 60
           MA+  ++  A ++   +     SSFHG P++ P  LRL   KS+ P   SISASAS  YD
Sbjct: 1   MAAMASTAFAPSVSSTTNKLFDSSFHGAPMS-PSLLRLQPIKSSRPNNLSISASASPPYD 60

Query: 61  LNQFNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAI 120
           LN F F PIKESIV+REMTRRYMTDMITYADTDV+VVGAGSAGLSCAYELSKNP++++AI
Sbjct: 61  LNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDELG++YDEQDNYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSK 180

Query: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLKSIGMI+ VPGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVAD 357
           APRMGPTFGAMMISGQKAAHLALKSLG  NA+DG      +     EL++AAA+S E AD
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGQPNALDG----TYVGGVHPELILAAADSAETAD 356

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BLAST of Moc10g12910.1 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 4.0e-153
Identity = 285/356 (80.06%), Postives = 311/356 (87.36%), Query Frame = 0


Query: 3   SSIASTLA--TNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASASYDLNQFN 62
           +S+A+TL   ++  KP+F D+ SSFHG P++        S      S+S+ + YDL  F 
Sbjct: 2   ASMATTLTSLSSNPKPAFFDNKSSFHGSPISYRVLPIKVSHQSPTISMSSVSPYDLQSFK 61

Query: 63  FSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQSV 122
           F PIKESIVAREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSKNPS+RVAIIEQSV
Sbjct: 62  FEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVAIIEQSV 121

Query: 123 SPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLARP 182
           SPGGGAWLGGQLFSAMVVRKPAH+FLDELG+EYDEQDNYVVIKHAALFTSTIMSKLLARP
Sbjct: 122 SPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLARP 181

Query: 183 NVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPF 242
           NVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPF
Sbjct: 182 NVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPF 241

Query: 243 GATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG 302
           GATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEIDGAPRMG
Sbjct: 242 GATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEIDGAPRMG 301

Query: 303 PTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVADA 357
           PTFGAMMISGQKAAHLAL++LG  NAIDG   + E    + EL++AAAE+ E+ DA
Sbjct: 302 PTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETM--QPELILAAAETGEIVDA 355

[Close]

BLAST of Moc10g12910.1 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.0e-149
Identity = 285/358 (79.61%), Postives = 313/358 (87.43%), Query Frame = 0


Query: 5   IASTLATNLHKPS-FLDSSSSFHGVPLAPPPSLRL---KSAAPAAFSISASAS--YDLNQ 64
           +ASTL +   + + F +S+SSF+G PLA P S+R+   K+ A  + S+S + S  YDL  
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSPPYDLKA 60

Query: 65  FNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQ 124
           F F PIKESIV+REMTRRYM DMITYADTDV+VVGAGS+GL C YELSKNPSV+VAIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIEQ 120

Query: 125 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLA 184
           SVSPGGGAWLGGQLFS MVVRKPAH FLDELG+EYDEQDNYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLA 180

Query: 185 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 244
           RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 245 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 304
           PFGATGVK L+SIGMID+VPGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAPR 300

Query: 305 MGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVADA 357
           MGPTFGAMMISGQKAAHLALK+LGL NA+DG      +     EL++AAA+S E+ADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGLPNALDGS----YVGGIHPELILAAADSAEIADA 352

[Close]

BLAST of Moc10g12910.1 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 2.7e-149
Identity = 279/356 (78.37%), Postives = 308/356 (86.52%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASA-SYDLNQFNF 62
           +++ S++ +      F    SSFHGVP+A    L    + P   +++A+A  YDL  F F
Sbjct: 2   ATLTSSICSKPKASVFDPHKSSFHGVPIATQARLSPVKSTPVNLAVTAAAMPYDLRSFKF 61

Query: 63  SPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQSVS 122
            PIKESIV+REMTRRYM DMITYADTDV+VVGAGSAGLSCAYELSKNPSV+VAIIEQSVS
Sbjct: 62  EPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAIIEQSVS 121

Query: 123 PGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLARPN 182
           PGGGAWLGGQLFS+MVVRKPAH FLDELG+EYDEQDNYVVIKHAALFTSTIMSKLLARPN
Sbjct: 122 PGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSKLLARPN 181

Query: 183 VKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFG 242
           VKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFG
Sbjct: 182 VKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFG 241

Query: 243 ATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGP 302
           ATGVKRL+S+GMIDSVPGMKALDMNTAED IVRLTREVVPGMIVTGMEVAEIDG+PRMGP
Sbjct: 242 ATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDGSPRMGP 301

Query: 303 TFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAEL-LIAAAESPEVADA 357
           TFGAMMISGQKAAHLALKSLGL NA+DG      + +   EL L AAA++ E+A+A
Sbjct: 302 TFGAMMISGQKAAHLALKSLGLPNALDG----TYIGNLHPELVLAAAADAAEIAEA 353

[Close]

BLAST of Moc10g12910.1 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.1e-147
Identity = 282/356 (79.21%), Postives = 308/356 (86.52%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPL-APPPSLRLKSAAPAAFSISA-SASYDLNQFN 62
           ++IASTL+ +  KP  L   SSFHG  + A P S+ LK   P +FS+ A +A YDLN F 
Sbjct: 2   AAIASTLSLSSTKPQRL-FDSSFHGSAISAAPISIGLK---PRSFSVRATTAGYDLNAFT 61

Query: 63  FSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQSV 122
           F PIKESIV+REMTRRYMTDMITYA+TDV+VVGAGSAGLS AYE+SKNP+V+VAIIEQSV
Sbjct: 62  FDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQSV 121

Query: 123 SPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLARP 182
           SPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQD YVV+KHAALFTSTIMSKLLARP
Sbjct: 122 SPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLARP 181

Query: 183 NVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPF 242
           NVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDGPF
Sbjct: 182 NVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDGPF 241

Query: 243 GATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG 302
           GATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG
Sbjct: 242 GATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG 301

Query: 303 PTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVADA 357
           PTFGAMMISGQKA  LALK+LGL NAIDG      + +   EL++AAA+S E  DA
Sbjct: 302 PTFGAMMISGQKAGQLALKALGLPNAIDG----TLVGNLSPELVLAAADSAETVDA 349

[Close]

BLAST of Moc10g12910.1 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 6.8e-164
Identity = 311/359 (86.63%), Postives = 327/359 (91.09%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASAS---YDLNQF 62
           +SIASTL T L  PS L   SSFHG PLA PPSLRLKS A  + +ISASAS   YDLNQF
Sbjct: 2   ASIASTLTTKLQIPSLL-HDSSFHGTPLASPPSLRLKSPAATSLTISASASQPPYDLNQF 61

Query: 63  NFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQS 122
            F+PI+ESIV+REMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSKNP++R+AIIEQS
Sbjct: 62  KFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIEQS 121

Query: 123 VSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLAR 182
           VSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQD+YVVIKHAALFTSTIMSKLLAR
Sbjct: 122 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 181

Query: 183 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 242
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 182 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 241

Query: 243 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM 302
           FGATGVKRLKSIG+IDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 242 FGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 301

Query: 303 GPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKD--EEAELLIAAAESPEVADA 357
           GPTFGAMMISGQKAAHLALKSLG  N+I GE +  E K   EE ELL+AA ESPEVADA
Sbjct: 302 GPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of Moc10g12910.1 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.0e-163
Identity = 309/359 (86.07%), Postives = 328/359 (91.36%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLKSAAPAAFSISASAS---YDLNQF 62
           +SIASTL T L KPS L   SSFHG PLA P SLRLKS A  + +ISASAS   YDLNQF
Sbjct: 2   ASIASTLTTKLQKPSLL-HDSSFHGTPLASPSSLRLKSTAATSLTISASASQPPYDLNQF 61

Query: 63  NFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQS 122
            F+PI+ESIV+REMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP++R+AIIEQS
Sbjct: 62  KFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIEQS 121

Query: 123 VSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLAR 182
           VSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQD+YVVIKHAALFTSTIMSKLLAR
Sbjct: 122 VSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAR 181

Query: 183 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 242
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 182 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 241

Query: 243 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM 302
           FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 242 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPRM 301

Query: 303 GPTFGAMMISGQKAAHLALKSLGLGNAI--DGERKKVEMKDEEAELLIAAAESPEVADA 357
           GPTFGAMMISGQKAAHLALKSLG  N+I  +GE  + ++  EE ELL+AA ESPEVADA
Sbjct: 302 GPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVADA 359

[Close]

BLAST of Moc10g12910.1 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.7e-162
Identity = 307/356 (86.24%), Postives = 322/356 (90.45%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLA-PPPSLRLKSAAPAAFSISASAS--YDLNQF 62
           +S+ASTL T L  PS     SSFHG PLA PPPSLRLKS  PAAFSISAS    YDLN F
Sbjct: 2   ASVASTLTTKLQTPSSSLLDSSFHGTPLAPPPPSLRLKS-TPAAFSISASQQQPYDLNSF 61

Query: 63  NFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQS 122
            F+PIKESIV+REMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP +R+AIIEQS
Sbjct: 62  KFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAIIEQS 121

Query: 123 VSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLAR 182
           VSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQ++YVVIKHAALFTSTIMSKLLAR
Sbjct: 122 VSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSKLLAR 181

Query: 183 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 242
           PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP
Sbjct: 182 PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGP 241

Query: 243 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM 302
           FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM
Sbjct: 242 FGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRM 301

Query: 303 GPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVAD 356
           GPTFGAMMISGQKAAHLALKSLGL N I+         DE A+L++AA ESPEVAD
Sbjct: 302 GPTFGAMMISGQKAAHLALKSLGLANCIE---------DEAADLILAAGESPEVAD 347

[Close]

BLAST of Moc10g12910.1 vs. ExPASy TrEMBL
Match: A0A6J1JNT3 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487533 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 8.3e-162
Identity = 310/363 (85.40%), Postives = 325/363 (89.53%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSS---SSFHGVPLA---PPPSLRLKSAAPAAFSISASAS--- 62
           +S+AST  T L  PS   SS   SSFHG PLA   PPPSLRLKS  PAAFSISASAS   
Sbjct: 2   ASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKS-TPAAFSISASASQHQ 61

Query: 63  -YDLNQFNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVR 122
            YDLN F F+PIKESIV+REMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSKNP +R
Sbjct: 62  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 121

Query: 123 VAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTI 182
           +AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+GVEYDEQ++YVVIKHAALFTSTI
Sbjct: 122 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 181

Query: 183 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 242
           MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 182 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 241

Query: 243 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 302
           SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 242 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 301

Query: 303 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPE 356
           IDGAPRMGPTFGAMMISGQKAAHLALKSLGL N I+         DE A+L++AA ESPE
Sbjct: 302 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIE---------DEAADLILAAGESPE 354

[Close]

BLAST of Moc10g12910.1 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.1e-161
Identity = 307/358 (85.75%), Postives = 327/358 (91.34%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPLAPPPSLRLK-SAAPAAFSISASAS---YDLNQ 62
           +SIAS+L T LH+PS LD  SSFHG PL P PS RLK + A A+ SISASA+   YDLNQ
Sbjct: 2   ASIASSLPTKLHRPSLLD--SSFHGSPLLPTPSFRLKPTTAAASLSISASAAQPPYDLNQ 61

Query: 63  FNFSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQ 122
           F F+PI+ESIV+REMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSK+PSVRVAIIEQ
Sbjct: 62  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 121

Query: 123 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLA 182
           SVSPGGGAWLGGQLFSAMVVRKPAHYFLDEL VEYDEQ++YVVIKHAALFTSTIMSKLLA
Sbjct: 122 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 181

Query: 183 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 242
           RPNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 182 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 241

Query: 243 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 302
           PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 242 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 301

Query: 303 MGPTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVADA 357
           MGPTFGAMMISGQKAAHLALK+LG  N+ID + +    K E AE+++AA E+PEVADA
Sbjct: 302 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKVEAAEMVLAAGETPEVADA 357

[Close]

BLAST of Moc10g12910.1 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 524.2 bits (1349), Expect = 7.9e-149
Identity = 282/356 (79.21%), Postives = 308/356 (86.52%), Query Frame = 0


Query: 3   SSIASTLATNLHKPSFLDSSSSFHGVPL-APPPSLRLKSAAPAAFSISA-SASYDLNQFN 62
           ++IASTL+ +  KP  L   SSFHG  + A P S+ LK   P +FS+ A +A YDLN F 
Sbjct: 2   AAIASTLSLSSTKPQRL-FDSSFHGSAISAAPISIGLK---PRSFSVRATTAGYDLNAFT 61

Query: 63  FSPIKESIVAREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKNPSVRVAIIEQSV 122
           F PIKESIV+REMTRRYMTDMITYA+TDV+VVGAGSAGLS AYE+SKNP+V+VAIIEQSV
Sbjct: 62  FDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAIIEQSV 121

Query: 123 SPGGGAWLGGQLFSAMVVRKPAHYFLDELGVEYDEQDNYVVIKHAALFTSTIMSKLLARP 182
           SPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQD YVV+KHAALFTSTIMSKLLARP
Sbjct: 122 SPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKLLARP 181

Query: 183 NVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPF 242
           NVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGHDGPF
Sbjct: 182 NVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGHDGPF 241

Query: 243 GATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG 302
           GATGVKRLKSIGMID VPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG
Sbjct: 242 GATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMG 301

Query: 303 PTFGAMMISGQKAAHLALKSLGLGNAIDGERKKVEMKDEEAELLIAAAESPEVADA 357
           PTFGAMMISGQKA  LALK+LGL NAIDG      + +   EL++AAA+S E  DA
Sbjct: 302 PTFGAMMISGQKAGQLALKALGLPNAIDG----TLVGNLSPELVLAAADSAETVDA 349

The following BLAST results are available for this feature:

BLAST of Moc10g12910.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008463440.1	1.4e-163	86.63	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo]	[more]
XP_004152916.1	4.1e-163	86.07	thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothet...	[more]
KAG7031400.1	6.9e-163	85.83	Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyr...	[more]
XP_038905461.1	9.1e-163	86.03	thiamine thiazole synthase, chloroplastic [Benincasa hispida]	[more]
KAG6600763.1	4.5e-162	85.32	Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]

BLAST of Moc10g12910.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	6.1e-154	80.61	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
F6H7K5	4.0e-153	80.06	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
Q38709	2.0e-149	79.61	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
F6H9A9	2.7e-149	78.37	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
Q38814	1.1e-147	79.21	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]

BLAST of Moc10g12910.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CJ81	6.8e-164	86.63	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A0A0L546	2.0e-163	86.07	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A6J1FWL6	3.7e-162	86.24	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1JNT3	8.3e-162	85.40	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1J7L4	1.1e-161	85.75	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Moc10g12910.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	7.9e-149	79.21	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01946	Thi4	coord: 71..303 e-value: 7.9E-114 score: 378.7
None	No IPR available	GENE3D	6.10.250.2840		coord: 55..85 e-value: 9.6E-18 score: 65.5
None	No IPR available	PANTHER	PTHR43422:SF6	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 6..356
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 6..356
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 86..321 e-value: 7.9E-122 score: 407.0
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 54..315
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 66..321 e-value: 9.1E-82 score: 272.1
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 6..330 score: 68.308296

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc10g12910	Moc10g12910	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc10g12910.1.exon1	Moc10g12910.1.exon1	exon
Moc10g12910.1.exon2	Moc10g12910.1.exon2	exon
Moc10g12910.1.exon3	Moc10g12910.1.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc10g12910.1	cds.Moc10g12910.1	CDS
cds.Moc10g12910.1	cds.Moc10g12910.1_2	CDS
cds.Moc10g12910.1	cds.Moc10g12910.1_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc10g12910.1	Moc10g12910.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0052837	thiazole biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0016763	pentosyltransferase activity