Moc09g12640.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g12640.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Gag-pro-like protein
Location	chr9: 10803097 .. 10803868 (-)
Sequence length	525
RNA-Seq Expression	Moc09g12640.1
Synteny	Moc09g12640.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGATCAAAAAGCTGAGCAGAAGAAAATAAGGAAAGACATTGAGGAGTTGCGCGAAAAGTTAGATGTCATCCTCTTCGCGTTAGAAAAAGGCAAGACGGTTGCGGATCCTGCTACATCTAGCAACCTCGTCCATGAGCCACAAGAAACCCCGCCTTATCCATTTGGGTACAGTCCACCTTTTAGGCCAGTAGCAGAGGGTTTCATGCCACAACATACAATGTACAACCCTCTGTATGATATTCCGGTTGGTTAGTACCCAGCTCCCTTTGTTAAGGGAGCTCAACAAATCCTAACCAACATCATTTTTCGAGAGCCCGAGCAAATGATGTCACCCCCTACAGTCCTTAATCTAGGTGGTCTCCTAGTCAAGACATACCCAGTCAGACAAAGTGCCTCCAGTAACGAGAAGTTCGAAGTTCTGTTGGAAAGATTAAGAGCAGTAGAGGGGACCTGGCAATATTGATGCCTCTCAATTGTGTTTGATATCTGGATTAGTCATCCCTTCGAAGTTTAAGGTGCTAGAATTCGAGAAATATGATGGTTCTTCTTGTCTTAAGAACCACCTTATAATGTACTGTAGGAAGATGACAGTGTACGTCCAAAATGACAAACTGTTGATACACTACATTCAAGATAGTTTATCTAGTCCAGCTTCTCGTTTGTACATGCAGTTGGACAGCTTTCATGTCGGCTCGTGGAAGAATCTGGTCGACTCATTCCTTAAGCAATATAAACATATAGATATGGCTCTAGATCGCTTATACTAG

mRNA sequence

ATGACAGATCAAAAAGCTGAGCAGAAGAAAATAAGGAAAGACATTGAGGAGTTGCGCGAAAAGTTAGATGTCATCCTCTTCGCGTTAGAAAAAGGCAAGACGGTTGCGGATCCTGCTACATCTAGCAACCTCGTCCATGAGCCACAAGAAACCCCGCCTTATCCATTTGGGTACAGTCCACCTTTTAGGCCAGTAGCAGAGGGTTTCATGCCACAACATACAATGTACAACCCTCTGTATGATATTCCGGTTGTCATCCCTTCGAAGTTTAAGGTGCTAGAATTCGAGAAATATGATGGTTCTTCTTGTCTTAAGAACCACCTTATAATGTACTGTAGGAAGATGACAGTGTACGTCCAAAATGACAAACTGTTGATACACTACATTCAAGATAGTTTATCTAGTCCAGCTTCTCGTTTGTACATGCAGTTGGACAGCTTTCATGTCGGCTCGTGGAAGAATCTGGTCGACTCATTCCTTAAGCAATATAAACATATAGATATGGCTCTAGATCGCTTATACTAG

Coding sequence (CDS)

ATGACAGATCAAAAAGCTGAGCAGAAGAAAATAAGGAAAGACATTGAGGAGTTGCGCGAAAAGTTAGATGTCATCCTCTTCGCGTTAGAAAAAGGCAAGACGGTTGCGGATCCTGCTACATCTAGCAACCTCGTCCATGAGCCACAAGAAACCCCGCCTTATCCATTTGGGTACAGTCCACCTTTTAGGCCAGTAGCAGAGGGTTTCATGCCACAACATACAATGTACAACCCTCTGTATGATATTCCGGTTGTCATCCCTTCGAAGTTTAAGGTGCTAGAATTCGAGAAATATGATGGTTCTTCTTGTCTTAAGAACCACCTTATAATGTACTGTAGGAAGATGACAGTGTACGTCCAAAATGACAAACTGTTGATACACTACATTCAAGATAGTTTATCTAGTCCAGCTTCTCGTTTGTACATGCAGTTGGACAGCTTTCATGTCGGCTCGTGGAAGAATCTGGTCGACTCATTCCTTAAGCAATATAAACATATAGATATGGCTCTAGATCGCTTATACTAG

Protein sequence

MTDQKAEQKKIRKDIEELREKLDVILFALEKGKTVADPATSSNLVHEPQETPPYPFGYSPPFRPVAEGFMPQHTMYNPLYDIPVVIPSKFKVLEFEKYDGSSCLKNHLIMYCRKMTVYVQNDKLLIHYIQDSLSSPASRLYMQLDSFHVGSWKNLVDSFLKQYKHIDMALDRLY

Homology

[Close]

BLAST of Moc09g12640.1 vs. NCBI nr
Match: XP_022157796.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia])

HSP 1 Score: 242.3 bits (617), Expect = 3.2e-60
Identity = 140/259 (54.05%), Postives = 145/259 (55.98%), Query Frame = 0


Query: 1   MTDQKAEQKKIRKDIEELREKLDVILFALEKGKTVADPATSSNLVHEPQETPPYPFGYSP 60
           M DQKAEQ+K RKDIEELREKLD I   LEKGK  ADPATSSN +HEPQETPPYP GY P
Sbjct: 1   MEDQKAEQEKTRKDIEELREKLDXIXLXLEKGKATADPATSSNPIHEPQETPPYPPGYGP 60

Query: 61  PFRPVAEGFMPQHTMYNPLYDIPV------------------------------------ 120
           P RPVAEGFMPQ+T YNPLYD+P+                                    
Sbjct: 61  PLRPVAEGFMPQYTTYNPLYDVPIGQYPHTFVKGAQQIPTNIIFREPEQMMSPPTVXNLG 120

Query: 121 -------------------------------------------------VIPSKFKVLEF 174
                                                            VIP KFKV EF
Sbjct: 121 GXPAKTDPVXQNAXSXEKXEVLEERLRAIEGTDVFGNIDASQLCLVSGLVIPPKFKVPEF 180

[Close]

BLAST of Moc09g12640.1 vs. NCBI nr
Match: XP_022155098.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica charantia])

HSP 1 Score: 198.7 bits (504), Expect = 4.0e-47
Identity = 123/264 (46.59%), Postives = 138/264 (52.27%), Query Frame = 0


Query: 3   DQKAEQKKIRKDIEELREKLDVILFALEKGKTVADPATSSNLVHEPQETPPYPFGY---- 62
           D+K+EQ+K RKDIEELREKLD IL ALEKGKT+A+   +SN +HEP  TP +P G+    
Sbjct: 4   DRKSEQEKTRKDIEELREKLDAILLALEKGKTIAE---TSNPIHEP-PTPQFPPGFEPSF 63

Query: 63  ---SPPFRPVAEGFMPQHTMYNPLYDIP-------------------------------- 122
               P FRP+ EGFM Q+T YNPLYDIP                                
Sbjct: 64  RPAGPSFRPMTEGFMSQYTTYNPLYDIPAGQFPPPLVRGPPQAPAGIIFQEPEQVIAPPT 123

Query: 123 -----------------------------------------------------VVIPSKF 174
                                                                +VIP KF
Sbjct: 124 VLNLGGPQANKDPERQEAPSSEKLEVLEERLRAVEGTDVFGNIDASQLCLXSGLVIPPKF 183

[Close]

BLAST of Moc09g12640.1 vs. NCBI nr
Match: XP_022143495.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia])

HSP 1 Score: 155.6 bits (392), Expect = 3.9e-34
Identity = 78/91 (85.71%), Postives = 79/91 (86.81%), Query Frame = 0


Query: 84  VVIPSKFKVLEFEKYDGSSCLKNHLIMYCRKMTVYVQNDKLLIHYIQDSLSSPASRLYMQ 143
           +VIP KFKV EFEKYDGSSC KNHLIMYCRKM  YVQNDKLLIH  QDSLSSPASR YMQ
Sbjct: 111 LVIPPKFKVPEFEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSSPASRWYMQ 170

Query: 144 LDSFHVGSWKNLVDSFLKQYKH-IDMALDRL 174
           LDS HVGSWKNL DSFLKQYKH IDMA DRL
Sbjct: 171 LDSSHVGSWKNLADSFLKQYKHNIDMAPDRL 201

[Close]

BLAST of Moc09g12640.1 vs. NCBI nr
Match: KAA0038145.1 (Gag-pro-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 155.2 bits (391), Expect = 5.1e-34
Identity = 86/182 (47.25%), Postives = 122/182 (67.03%), Query Frame = 0


Query: 1   MTDQKAEQKKIRKDIEELREKLDVI--LFALEKGKTVADPATSSNLVHEPQETPPYPFGY 60
           M ++  +  K+R++I  L E++  I  L ++ KGK V D   SSN + +  + P +P G+
Sbjct: 1   MEEKDKDMDKMRQEINNLGEQVSKILELLSMGKGKVVVDTIQSSNPIQDTDD-PIFPPGF 60

Query: 61  SPPFRPVAEGFMPQHTM-YNPLYDIP-----VVIPSKFKVLEFEKYDGSSCLKNHLIMYC 120
           +P    V +    QH +  NPLY +P     ++IP+KFKV EF+KYDGS+C ++HLIMYC
Sbjct: 61  TPYHMNVPQSQTIQHYVPTNPLYVVPPIVPGLIIPAKFKVPEFDKYDGSTCPRSHLIMYC 120

Query: 121 RKMTVYVQNDKLLIHYIQDSLSSPASRLYMQLDSFHVGSWKNLVDSFLKQYKH-IDMALD 174
           RK+  ++ NDKLL+H  QDSL+  ASR Y+QLD+ H+  WK+L D+FLKQYKH IDMA D
Sbjct: 121 RKIAAHINNDKLLVHCFQDSLTGSASRWYIQLDNAHIHVWKDLADAFLKQYKHSIDMAPD 180

[Close]

BLAST of Moc09g12640.1 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 151.4 bits (381), Expect = 7.3e-33
Identity = 76/91 (83.52%), Postives = 78/91 (85.71%), Query Frame = 0


Query: 84  VVIPSKFKVLEFEKYDGSSCLKNHLIMYCRKMTVYVQNDKLLIHYIQDSLSSPASRLYMQ 143
           +VIP KFKV EFEKYDGSSC KNHLIMYCRKM  YVQNDKLLIH  QDSLS PASR YMQ
Sbjct: 60  LVIPPKFKVPEFEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSGPASRWYMQ 119

Query: 144 LDSFHVGSWKNLVDSFLKQYKH-IDMALDRL 174
           LDS +VGSWKNL DSFLKQYKH IDMA DRL
Sbjct: 120 LDSSNVGSWKNLADSFLKQYKHNIDMAPDRL 150

[Close]

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL
Match: A0A6J1DZ90 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.5e-60
Identity = 140/259 (54.05%), Postives = 145/259 (55.98%), Query Frame = 0


Query: 1   MTDQKAEQKKIRKDIEELREKLDVILFALEKGKTVADPATSSNLVHEPQETPPYPFGYSP 60
           M DQKAEQ+K RKDIEELREKLD I   LEKGK  ADPATSSN +HEPQETPPYP GY P
Sbjct: 1   MEDQKAEQEKTRKDIEELREKLDXIXLXLEKGKATADPATSSNPIHEPQETPPYPPGYGP 60

Query: 61  PFRPVAEGFMPQHTMYNPLYDIPV------------------------------------ 120
           P RPVAEGFMPQ+T YNPLYD+P+                                    
Sbjct: 61  PLRPVAEGFMPQYTTYNPLYDVPIGQYPHTFVKGAQQIPTNIIFREPEQMMSPPTVXNLG 120

Query: 121 -------------------------------------------------VIPSKFKVLEF 174
                                                            VIP KFKV EF
Sbjct: 121 GXPAKTDPVXQNAXSXEKXEVLEERLRAIEGTDVFGNIDASQLCLVSGLVIPPKFKVPEF 180

[Close]

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL
Match: A0A6J1DM29 (LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia OX=3673 GN=LOC111022231 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.1e-47
Identity = 124/264 (46.97%), Postives = 138/264 (52.27%), Query Frame = 0


Query: 3   DQKAEQKKIRKDIEELREKLDVILFALEKGKTVADPATSSNLVHEPQETPPYPFGY---- 62
           D+K+EQ+K RKDIEELREKLD IL ALEKGKT+A+   +SN +HEP  TP +P G+    
Sbjct: 4   DRKSEQEKTRKDIEELREKLDAILLALEKGKTIAE---TSNPIHEP-PTPQFPPGFEPSF 63

Query: 63  ---SPPFRPVAEGFMPQHTMYNPLYDIP-------------------------------- 122
               P FRP+ EGFM Q+T YNPLYDIP                                
Sbjct: 64  RPAGPSFRPMTEGFMSQYTTYNPLYDIPAGQFPPPLVRGPPQAPAGIIFQEPEQVIAPPT 123

Query: 123 -----------------------------------------------------VVIPSKF 174
                                                                +VIP KF
Sbjct: 124 VLNLGGPQANKDPERQEAPSSEKLEVLEERLRAVEGTDVFGNIDASQLCLXSGLVIPPKF 183

[Close]

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL
Match: A0A6J1CNY7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.9e-34
Identity = 78/91 (85.71%), Postives = 79/91 (86.81%), Query Frame = 0


Query: 84  VVIPSKFKVLEFEKYDGSSCLKNHLIMYCRKMTVYVQNDKLLIHYIQDSLSSPASRLYMQ 143
           +VIP KFKV EFEKYDGSSC KNHLIMYCRKM  YVQNDKLLIH  QDSLSSPASR YMQ
Sbjct: 111 LVIPPKFKVPEFEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSSPASRWYMQ 170

Query: 144 LDSFHVGSWKNLVDSFLKQYKH-IDMALDRL 174
           LDS HVGSWKNL DSFLKQYKH IDMA DRL
Sbjct: 171 LDSSHVGSWKNLADSFLKQYKHNIDMAPDRL 201

[Close]

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL
Match: A0A5A7T9N7 (Gag-pro-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G003730 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 2.5e-34
Identity = 86/182 (47.25%), Postives = 122/182 (67.03%), Query Frame = 0


Query: 1   MTDQKAEQKKIRKDIEELREKLDVI--LFALEKGKTVADPATSSNLVHEPQETPPYPFGY 60
           M ++  +  K+R++I  L E++  I  L ++ KGK V D   SSN + +  + P +P G+
Sbjct: 1   MEEKDKDMDKMRQEINNLGEQVSKILELLSMGKGKVVVDTIQSSNPIQDTDD-PIFPPGF 60

Query: 61  SPPFRPVAEGFMPQHTM-YNPLYDIP-----VVIPSKFKVLEFEKYDGSSCLKNHLIMYC 120
           +P    V +    QH +  NPLY +P     ++IP+KFKV EF+KYDGS+C ++HLIMYC
Sbjct: 61  TPYHMNVPQSQTIQHYVPTNPLYVVPPIVPGLIIPAKFKVPEFDKYDGSTCPRSHLIMYC 120

Query: 121 RKMTVYVQNDKLLIHYIQDSLSSPASRLYMQLDSFHVGSWKNLVDSFLKQYKH-IDMALD 174
           RK+  ++ NDKLL+H  QDSL+  ASR Y+QLD+ H+  WK+L D+FLKQYKH IDMA D
Sbjct: 121 RKIAAHINNDKLLVHCFQDSLTGSASRWYIQLDNAHIHVWKDLADAFLKQYKHSIDMAPD 180

[Close]

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 3.6e-33
Identity = 76/91 (83.52%), Postives = 78/91 (85.71%), Query Frame = 0


Query: 84  VVIPSKFKVLEFEKYDGSSCLKNHLIMYCRKMTVYVQNDKLLIHYIQDSLSSPASRLYMQ 143
           +VIP KFKV EFEKYDGSSC KNHLIMYCRKM  YVQNDKLLIH  QDSLS PASR YMQ
Sbjct: 60  LVIPPKFKVPEFEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSGPASRWYMQ 119

Query: 144 LDSFHVGSWKNLVDSFLKQYKH-IDMALDRL 174
           LDS +VGSWKNL DSFLKQYKH IDMA DRL
Sbjct: 120 LDSSNVGSWKNLADSFLKQYKHNIDMAPDRL 150

The following BLAST results are available for this feature:

BLAST of Moc09g12640.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157796.1	3.2e-60	54.05	LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia]	[more]
XP_022155098.1	4.0e-47	46.59	LOW QUALITY PROTEIN: uncharacterized protein LOC111022231, partial [Momordica ch...	[more]
XP_022143495.1	3.9e-34	85.71	LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia]	[more]
KAA0038145.1	5.1e-34	47.25	Gag-pro-like protein [Cucumis melo var. makuwa]	[more]
XP_022158986.1	7.3e-33	83.52	LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]	[more]

BLAST of Moc09g12640.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DZ90	1.5e-60	54.05	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1	[more]
A0A6J1DM29	1.1e-47	46.97	LOW QUALITY PROTEIN: uncharacterized protein LOC111022231 OS=Momordica charantia...	[more]
A0A6J1CNY7	1.9e-34	85.71	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1	[more]
A0A5A7T9N7	2.5e-34	47.25	Gag-pro-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G...	[more]
A0A6J1E2J7	3.6e-33	83.52	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1	[more]

BLAST of Moc09g12640.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 1..21

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc09g12640	Moc09g12640	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc09g12640.1.exon2	Moc09g12640.1.exon2	exon
Moc09g12640.1.exon1	Moc09g12640.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc09g12640.1	cds.Moc09g12640.1_2	CDS
cds.Moc09g12640.1	cds.Moc09g12640.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc09g12640.1	Moc09g12640.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity