Moc09g12100.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g12100.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr9: 10290132 .. 10290743 (-)
Sequence length	543
RNA-Seq Expression	Moc09g12100.1
Synteny	Moc09g12100.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTACTCGGGTGTGCACTATGGAGGAGACTTACAACAAATTAATGCGAGCCAATCGCACAGGATCCCAATCACGGATCCAAGGGGATGGGGAGGACCAGCACTACGAAGGCGAGACTAGGGATCCTTCTCTCCACCCAGAGAATGACGATCAACACCCTGTCGATAATGATGGGGCCAATTATAGTCGTCAGGAGGACGACTTAAGGAAAGTTCTCATCGATAAGAAGAAGAAGAACTCTGCCTTCTGAGGTATGGACGTCTCTCTTTCTTGTTCGTAGAAGAACTCGAACTTGAATGATGAATCTCATTACACTCCGATTGTACCTGAAGGAGTTATTACAAGGGAAGAGTTCGACCTAATGAAAAGCAAGTTTGACGCTCAGATCAAGACTTTCAAACAAAAGTACGAGAAGAAAGAAAGTGTGTTTGACGATGGCGAAATGGGCGAATTGTCGTTCACTTCGGACATCCTTGAGGCACCCATCCTTGCCAAATTCAAGACATCCGCCATGAAGCCTTACGATGAGTCAAATGACCCAAAAGATTATGTTGAGGTCTTTGAGGGCCTCATGGACTTCCAAGCGGCATCTGATGCCATCAAGTGCTGA

mRNA sequence

ATGCGTACTCGGGTGTGCACTATGGAGGAGACTTACAACAAATTAATGCGAGCCAATCGCACAGGATCCCAATCACGGATCCAAGGGGATGGGGAGGACCAGCACTACGAAGGCGAGACTAGGGATCCTTCTCTCCACCCAGAGAATGACGATCAACACCCTGTCGATAATGATGGGGCCAATTATAGTCGTCAGGAGGACGACTTAAGGAAAAAGAACTCGAACTTGAATGATGAATCTCATTACACTCCGATTGTACCTGAAGGAGTTATTACAAGGGAAGAGTTCGACCTAATGAAAAGCAAGTTTGACGCTCAGATCAAGACTTTCAAACAAAAGTACGAGAAGAAAGAAAGTGTGTTTGACGATGGCGAAATGGGCGAATTGTCGTTCACTTCGGACATCCTTGAGGCACCCATCCTTGCCAAATTCAAGACATCCGCCATGAAGCCTTACGATGAGTCAAATGACCCAAAAGATTATGTTGAGGTCTTTGAGGGCCTCATGGACTTCCAAGCGGCATCTGATGCCATCAAGTGCTGA

Coding sequence (CDS)

ATGCGTACTCGGGTGTGCACTATGGAGGAGACTTACAACAAATTAATGCGAGCCAATCGCACAGGATCCCAATCACGGATCCAAGGGGATGGGGAGGACCAGCACTACGAAGGCGAGACTAGGGATCCTTCTCTCCACCCAGAGAATGACGATCAACACCCTGTCGATAATGATGGGGCCAATTATAGTCGTCAGGAGGACGACTTAAGGAAAAAGAACTCGAACTTGAATGATGAATCTCATTACACTCCGATTGTACCTGAAGGAGTTATTACAAGGGAAGAGTTCGACCTAATGAAAAGCAAGTTTGACGCTCAGATCAAGACTTTCAAACAAAAGTACGAGAAGAAAGAAAGTGTGTTTGACGATGGCGAAATGGGCGAATTGTCGTTCACTTCGGACATCCTTGAGGCACCCATCCTTGCCAAATTCAAGACATCCGCCATGAAGCCTTACGATGAGTCAAATGACCCAAAAGATTATGTTGAGGTCTTTGAGGGCCTCATGGACTTCCAAGCGGCATCTGATGCCATCAAGTGCTGA

Protein sequence

MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGANYSRQEDDLRKKNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKKESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDAIKC

Homology

[Close]

BLAST of Moc09g12100.1 vs. NCBI nr
Match: XP_022151719.1 (uncharacterized protein LOC111019634 [Momordica charantia])

HSP 1 Score: 174.9 bits (442), Expect = 6.4e-40
Identity = 105/200 (52.50%), Postives = 125/200 (62.50%), Query Frame = 0


Query: 1   MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGA 60
           MRT++CTME+ Y+++++A    S+S  +    D H   E R   L P     HP   +  
Sbjct: 1   MRTQMCTMEKMYSEMVQAAGARSRSENRVARNDMH---EQRGHHLGPVKKG-HPEGGEDE 60

Query: 61  NYSRQEDDLRK--------------------KNSNLNDESHYTPIVPEGVITREEFDLMK 120
            Y+ Q  DLR+                    +NSN   ES Y PI PEGVITREEFD +K
Sbjct: 61  EYTHQRGDLREHLNRKRSSSLRKGQSPSCSHRNSNQQAESSYNPITPEGVITREEFDQLK 120

Query: 121 SKFDAQIKTFKQKYEKKESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKD 180
           SKFDAQ++  K K EKKES FDDG++GE  FTSDILEA I  KFKT  MKPYD S DPKD
Sbjct: 121 SKFDAQVEALKAKCEKKESSFDDGDLGESPFTSDILEALIPLKFKTPTMKPYDGSKDPKD 180

[Close]

BLAST of Moc09g12100.1 vs. NCBI nr
Match: XP_022156088.1 (uncharacterized protein LOC111023060 [Momordica charantia])

HSP 1 Score: 167.5 bits (423), Expect = 1.0e-37
Identity = 101/190 (53.16%), Postives = 126/190 (66.32%), Query Frame = 0


Query: 1   MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGA 60
           MRT++ TMEE YNK+++     S+S  Q   ED H +G+     LH +  D+  +  D  
Sbjct: 4   MRTQMRTMEEMYNKMVQIAGARSRSGDQVVHEDVHEQGD-----LHCDPVDEEHLGGDLR 63

Query: 61  NY-SRQEDDLRK---------KNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTF 120
           ++ +R+ +   +         KNSN   ES Y PI PEGVITREEF+ +KSKFDAQ++  
Sbjct: 64  DHLNRKRNSSHRGERTSTYWHKNSNQQAESSYNPIAPEGVITREEFNQLKSKFDAQVEAL 123

Query: 121 KQKYEKKESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMD 180
           K + EKKES FDDG++GE  FTSDILEA I  KFKT  MK YD S DPKDYVEVFEGLMD
Sbjct: 124 KVRCEKKESAFDDGDLGESPFTSDILEASIPPKFKTPTMKSYDGSKDPKDYVEVFEGLMD 183

[Close]

BLAST of Moc09g12100.1 vs. NCBI nr
Match: XP_022155139.1 (uncharacterized protein LOC111022280 [Momordica charantia])

HSP 1 Score: 163.3 bits (412), Expect = 1.9e-36
Identity = 91/167 (54.49%), Postives = 109/167 (65.27%), Query Frame = 0


Query: 38  GETRDPSLHPENDDQHPVDND-GANYSRQEDDLRK-----------------------KN 97
           GE   PS+ P N  + P+ ND G +YS +++DLRK                        N
Sbjct: 9   GEKGAPSIQPGN--REPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREFSN 68

Query: 98  SNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKKESVFDDGEMGELSFTS 157
           SNL  +S Y P++PE VI REEFDLMK +FD Q++  K + EKKES FDD ++GE  FTS
Sbjct: 69  SNLKAQSKYKPLIPEAVINREEFDLMKHRFDEQVEALKARCEKKESPFDDDDLGESPFTS 128

Query: 158 DILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDAIKC 181
           DI+EAPI  KFKT  MKPYD S DPKDYVEVFEGLMDFQAA+DAIKC
Sbjct: 129 DIMEAPIPPKFKTPTMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKC 173

[Close]

BLAST of Moc09g12100.1 vs. NCBI nr
Match: XP_022159327.1 (uncharacterized protein LOC111025738 [Momordica charantia])

HSP 1 Score: 161.0 bits (406), Expect = 9.6e-36
Identity = 95/179 (53.07%), Postives = 117/179 (65.36%), Query Frame = 0


Query: 1   MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGA 60
           MRT++ TMEE YN++++A   GS+S    D   +   G+ RD      +       +   
Sbjct: 99  MRTQMLTMEEMYNEMVQAVGAGSRSE---DRAARDERGDLRDHLSRKRSSSLRKGRSPSC 158

Query: 61  NYSRQEDDLRKKNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKKESV 120
           ++         KNSN   ES Y P+VPEGVITREEFD +KSKFDAQ++T K + E K S 
Sbjct: 159 SH---------KNSNQQAESSYNPVVPEGVITREEFDQLKSKFDAQVETLKARCEVKGST 218

Query: 121 FDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDAIK 180
           FDDG++GE  FTSDILEA I +KFKT  MKPYD S DPKDYVEVFEGLM FQAA+DAIK
Sbjct: 219 FDDGDLGESPFTSDILEALIPSKFKTPTMKPYDGSKDPKDYVEVFEGLMGFQAATDAIK 265

[Close]

BLAST of Moc09g12100.1 vs. NCBI nr
Match: XP_022157897.1 (uncharacterized protein LOC111024503 [Momordica charantia])

HSP 1 Score: 151.8 bits (382), Expect = 5.8e-33
Identity = 78/123 (63.41%), Postives = 89/123 (72.36%), Query Frame = 0


Query: 58  DGANYSRQEDDLRKKNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKK 117
           D  +YSR+       NS+   +S Y P+ PE VITREEFDLMK +FD Q++  K K EKK
Sbjct: 475 DSPSYSRE-----FSNSDFKAQSKYKPLTPEAVITREEFDLMKHRFDEQVEALKAKCEKK 534

Query: 118 ESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDA 177
           E  FDDG++GE  FTSDILE PI  KFKT  MK YD S DPKDYVEVFEGLMDFQAA+DA
Sbjct: 535 ECSFDDGDLGESPFTSDILEVPIPPKFKTPTMKLYDRSKDPKDYVEVFEGLMDFQAATDA 592

Query: 178 IKC 181
           IKC
Sbjct: 595 IKC 592

[Close]

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL
Match: A0A6J1DDW5 (uncharacterized protein LOC111019634 OS=Momordica charantia OX=3673 GN=LOC111019634 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 3.1e-40
Identity = 105/200 (52.50%), Postives = 125/200 (62.50%), Query Frame = 0


Query: 1   MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGA 60
           MRT++CTME+ Y+++++A    S+S  +    D H   E R   L P     HP   +  
Sbjct: 1   MRTQMCTMEKMYSEMVQAAGARSRSENRVARNDMH---EQRGHHLGPVKKG-HPEGGEDE 60

Query: 61  NYSRQEDDLRK--------------------KNSNLNDESHYTPIVPEGVITREEFDLMK 120
            Y+ Q  DLR+                    +NSN   ES Y PI PEGVITREEFD +K
Sbjct: 61  EYTHQRGDLREHLNRKRSSSLRKGQSPSCSHRNSNQQAESSYNPITPEGVITREEFDQLK 120

Query: 121 SKFDAQIKTFKQKYEKKESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKD 180
           SKFDAQ++  K K EKKES FDDG++GE  FTSDILEA I  KFKT  MKPYD S DPKD
Sbjct: 121 SKFDAQVEALKAKCEKKESSFDDGDLGESPFTSDILEALIPLKFKTPTMKPYDGSKDPKD 180

[Close]

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL
Match: A0A6J1DPN4 (uncharacterized protein LOC111023060 OS=Momordica charantia OX=3673 GN=LOC111023060 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 5.0e-38
Identity = 101/190 (53.16%), Postives = 126/190 (66.32%), Query Frame = 0


Query: 1   MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGA 60
           MRT++ TMEE YNK+++     S+S  Q   ED H +G+     LH +  D+  +  D  
Sbjct: 4   MRTQMRTMEEMYNKMVQIAGARSRSGDQVVHEDVHEQGD-----LHCDPVDEEHLGGDLR 63

Query: 61  NY-SRQEDDLRK---------KNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTF 120
           ++ +R+ +   +         KNSN   ES Y PI PEGVITREEF+ +KSKFDAQ++  
Sbjct: 64  DHLNRKRNSSHRGERTSTYWHKNSNQQAESSYNPIAPEGVITREEFNQLKSKFDAQVEAL 123

Query: 121 KQKYEKKESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMD 180
           K + EKKES FDDG++GE  FTSDILEA I  KFKT  MK YD S DPKDYVEVFEGLMD
Sbjct: 124 KVRCEKKESAFDDGDLGESPFTSDILEASIPPKFKTPTMKSYDGSKDPKDYVEVFEGLMD 183

[Close]

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL
Match: A0A6J1DPC9 (uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022280 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 9.3e-37
Identity = 91/167 (54.49%), Postives = 109/167 (65.27%), Query Frame = 0


Query: 38  GETRDPSLHPENDDQHPVDND-GANYSRQEDDLRK-----------------------KN 97
           GE   PS+ P N  + P+ ND G +YS +++DLRK                        N
Sbjct: 9   GEKGAPSIQPGN--REPIPNDEGVDYSLRDNDLRKHLTDKKKKASWEPEDSLSYSREFSN 68

Query: 98  SNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKKESVFDDGEMGELSFTS 157
           SNL  +S Y P++PE VI REEFDLMK +FD Q++  K + EKKES FDD ++GE  FTS
Sbjct: 69  SNLKAQSKYKPLIPEAVINREEFDLMKHRFDEQVEALKARCEKKESPFDDDDLGESPFTS 128

Query: 158 DILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDAIKC 181
           DI+EAPI  KFKT  MKPYD S DPKDYVEVFEGLMDFQAA+DAIKC
Sbjct: 129 DIMEAPIPPKFKTPTMKPYDGSKDPKDYVEVFEGLMDFQAATDAIKC 173

[Close]

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL
Match: A0A6J1DZJ1 (uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025738 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.6e-36
Identity = 95/179 (53.07%), Postives = 117/179 (65.36%), Query Frame = 0


Query: 1   MRTRVCTMEETYNKLMRANRTGSQSRIQGDGEDQHYEGETRDPSLHPENDDQHPVDNDGA 60
           MRT++ TMEE YN++++A   GS+S    D   +   G+ RD      +       +   
Sbjct: 99  MRTQMLTMEEMYNEMVQAVGAGSRSE---DRAARDERGDLRDHLSRKRSSSLRKGRSPSC 158

Query: 61  NYSRQEDDLRKKNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKKESV 120
           ++         KNSN   ES Y P+VPEGVITREEFD +KSKFDAQ++T K + E K S 
Sbjct: 159 SH---------KNSNQQAESSYNPVVPEGVITREEFDQLKSKFDAQVETLKARCEVKGST 218

Query: 121 FDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDAIK 180
           FDDG++GE  FTSDILEA I +KFKT  MKPYD S DPKDYVEVFEGLM FQAA+DAIK
Sbjct: 219 FDDGDLGESPFTSDILEALIPSKFKTPTMKPYDGSKDPKDYVEVFEGLMGFQAATDAIK 265

[Close]

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL
Match: A0A6J1DVR0 (uncharacterized protein LOC111024503 OS=Momordica charantia OX=3673 GN=LOC111024503 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.8e-33
Identity = 78/123 (63.41%), Postives = 89/123 (72.36%), Query Frame = 0


Query: 58  DGANYSRQEDDLRKKNSNLNDESHYTPIVPEGVITREEFDLMKSKFDAQIKTFKQKYEKK 117
           D  +YSR+       NS+   +S Y P+ PE VITREEFDLMK +FD Q++  K K EKK
Sbjct: 475 DSPSYSRE-----FSNSDFKAQSKYKPLTPEAVITREEFDLMKHRFDEQVEALKAKCEKK 534

Query: 118 ESVFDDGEMGELSFTSDILEAPILAKFKTSAMKPYDESNDPKDYVEVFEGLMDFQAASDA 177
           E  FDDG++GE  FTSDILE PI  KFKT  MK YD S DPKDYVEVFEGLMDFQAA+DA
Sbjct: 535 ECSFDDGDLGESPFTSDILEVPIPPKFKTPTMKLYDRSKDPKDYVEVFEGLMDFQAATDA 592

Query: 178 IKC 181
           IKC
Sbjct: 595 IKC 592

The following BLAST results are available for this feature:

BLAST of Moc09g12100.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151719.1	6.4e-40	52.50	uncharacterized protein LOC111019634 [Momordica charantia]	[more]
XP_022156088.1	1.0e-37	53.16	uncharacterized protein LOC111023060 [Momordica charantia]	[more]
XP_022155139.1	1.9e-36	54.49	uncharacterized protein LOC111022280 [Momordica charantia]	[more]
XP_022159327.1	9.6e-36	53.07	uncharacterized protein LOC111025738 [Momordica charantia]	[more]
XP_022157897.1	5.8e-33	63.41	uncharacterized protein LOC111024503 [Momordica charantia]	[more]

BLAST of Moc09g12100.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DDW5	3.1e-40	52.50	uncharacterized protein LOC111019634 OS=Momordica charantia OX=3673 GN=LOC111019...	[more]
A0A6J1DPN4	5.0e-38	53.16	uncharacterized protein LOC111023060 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DPC9	9.3e-37	54.49	uncharacterized protein LOC111022280 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DZJ1	4.6e-36	53.07	uncharacterized protein LOC111025738 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1DVR0	2.8e-33	63.41	uncharacterized protein LOC111024503 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]

BLAST of Moc09g12100.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 16..83
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 30..77

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc09g12100	Moc09g12100	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc09g12100.1.exon2	Moc09g12100.1.exon2	exon
Moc09g12100.1.exon1	Moc09g12100.1.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc09g12100.1	cds.Moc09g12100.1_2	CDS
cds.Moc09g12100.1	cds.Moc09g12100.1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc09g12100.1	Moc09g12100.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane