Moc09g05640.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc09g05640.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	LOW QUALITY PROTEIN: putative disease resistance protein RGA3
Location	chr9: 4322394 .. 4328749 (+)
Sequence length	546
RNA-Seq Expression	Moc09g05640.1
Synteny	Moc09g05640.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGAATCTATTCTCTTCAGCCTTACAGGAAAAATTATAATGAAATTGGGGTCTTTCGCAGGCCAAGAGCTTGCATTGCTCTGGGGCGTGGTTAATGAGCTTGACAAGCTCAAACGCATGCTTTCTGCCATTCAAGCCGTGGTTCTGGATGCAGAGGAGCAGCAGTTCAAGAGCCATGCAGTCAAGGATTGGATTTCAAAGCTTAAAGATGCTCTCTACGACATTGAGGACCTATTTGATGAGCTCTCTTATGCAACCCTGAGAATGCAAGTTATGGCTAGAGATAATAGAAGAAAGACCTACAAACAGGTATGCATGTTCTTCTCCAAATCGAATCCTATTGCTTTCGCTCTCAAAATGGGTCACAAAATCAAGGCAATTAGGGAGAGACTAGCAGATATTGGTATCGAGAAAACCCAATTTAAATTCTCTGAGTGTGTGATTATAAATATAAGAGATGATGGATTGAGGAAGACACGAGAGACTGGCTCTTATATACCTGAAGAGGAAGTGATTGATAGGAATGATGACAAGAAAGCAATAATAAATCTGCTATTAAAACCCAACACCAACAGAGATCCTGTAATTTCTATAGTTGGAATGGGAGGATTAGGAAAGACTGCCCTTGCTCAATCTATCTATAATGATAACATGTTGGTCGAACATTTTGATTTGAAGTTATGGGTGTGTGTTTCTGAAGAATTTGAAGTAAAAGTTATAATCGAAAAAATAATAGAGTCTGCAACGGGGAAAAGACCTGAGTCATGCCTTCAAATGGATTCCTTGCAAAGTAAACTTAGAAAGCTAGTTGATGTGAAAAGAAATATTTGCTCATCATGGATGATGTGTGGAATGAGGATCATGAGAAATGGGTAATTCTGAGACAATCCTTGATAGATGGTGCAAAAGGGAGTAGAATTTTGATCACAACGCGTAGTGAACAAGTTGCAAAAACTTTTGACTCATCTCCATTGTATTTTGTAGAAAGCTTGGATGAAGACAACTCTTGGTTATTGTTTAAGAAAATGACATTTTGTAGACAATCAGAACAAGATCTTATGAATTCAAATTTAGTCGAAATTGGTAAAGAGATTGTGGCAAAATTAAAAGGTGTCCCTCTTGCAATAAAAACTATAGGACGACTTTTGTATGCTAAAAAGTCAGAAGATCGTCTTTCAAAGATAAGGAACTTTACCAAATTACGGAACAAAGAGGTTTAGAAGACCTGCGATCAATACTTGAGCTCAGTTATATCCACCTCCCGTCTAATTTGAAGCAATGTTTTCTGTATTGTGCTTTGTTCCCTAAAAATTATGAAATTCGAAAGGATGAAGTAATAATGCAATGGAGAGCACAAGGTTTCATTCAACCAAACGACAAAGATAAGCTCGCTGATATTGGTGAGGATTATTTTATGGAGCTATTATCGAGGTCATTCTTTCAAGACGTCACAAAAAAAGAATTGGGAATCATAAAAGCATGTAAGATACATGACTTGATGCATGATCTTGCTTGTTCGATAGTAGATAAAGAATGTGCCCGTGACGTGAATGCTTGCACATTCAACGAAAGAACTCGTCACATTTCATTTGACTTAATGTCATGGAAAGAATTGGAAAAGCTTGCAAAAACATTATATGAGGCAAGAAATTTGAGGACATGTTTTCTTCGAACTAATCAAATTTCTTTCTTCGAAAGAGTGAACAATTTAAATGAAATCTTTGAGTGTGTCTTTCATAATCTTTTGAGACTACGGGCATTGGACTTGCAATTGAGTATTTACTTCCAGAGTGCCGAGTTTCTCAAATGTATTAAGACATTGAAACATTTGAGATATCTGAAAATTAAAATTACTGGTAAAGTTACAAAAAAAAATTTCAAATTCTATTACTGGATTGTACCATTTGGAAGGACTTATCCTCAGATGTGTAGGATTAAAAGAATTGCCGAAAGATGTAAAAAATTGGATCAATCTTAAGCATCTTGATCTTTCAAGTAGTGGGGTAGAATTTCTTCCGGATTCTATTACTCAGTAAAAGAACTTGGAAACCCTGATGCTTTGTTTTTGTTATAAATTAAAAGAACTACCCACTGATACGAAAAACTTGATCAACCTTAAACATCTTGATGTGGATGGATGCACTGCTTTGACTCATATGCCAAGAGGACTAGGTGAGCTAACTAATCTTCAAACATTGAATTTATTTGTATTAGAAAAATATAATAATGGTGGTGGTGGACTAAGCGAGTTGAATGGACTTAACAAGCTGAGAGGATCTCTAACTATCGAAGGATTGCAATTTTGTTCAACTGTTGATCTACAAACGAATGCTAAGTTCCTGGAACTAAAGTCGGGTCTTCAAAAGTTAGTGTTAAAGTGGAAGCGGGACGACGATTCATTTAATGATCCTACCATCAATGCCTTGGATGATGACTACAAAAGGGTTTTAGAATGCAGCCACATCCAAATACTCAAGAAATATGTATAGTGGGATACAAAGGAGTGAAGTTATGTAATTGGCTATCCTCGTCCTCTTATTCCTTTGGTGATCTAGTCCGCATGAAAGTTTCATCATGTGAAAGATTGCAGCATCTCCCTCAATTTGATCCATTTCCTAATCTCAAGGTCCTTCATCTTGAAGACTTGCCCTGTATCGAGTACATTGTAAGCAACAATGATTATCCTTGTTCATCCACTTTCTTTCCATCCCTCGAGGTTTTAATCATTTCAGACACGCCTAAATTGAGGGGATGGTTGAAGAAGGGTCAAACCTCAACAAATGATTGTATATTTCCATGCCTTTCTGAATTGTATGTTTTGGATTGTCCTCTGTTTGCTTCGATGGCATGTCGTGCACCTTCCAATTCATTGGAAACAATGGATTCAATACTATACCATATTGACGTGGAAGGATTATTGGATATTGTACCATTTAAAGTAAATTTTCCAAGACGTCCCTCCGGTCTACACCTTTGGCTCATTTCCGAAGGGGAGTTTTTGTCCAATCATCTGCAACGTGGGGCAAATCTTCACCAATTATTTATACATTTCTGTCCAAATCTAACGAGTCTACTAGAATGGATCGGCAACATCACATCACTCTCAAAATTGGAGATTATGAATGGTTCAGATTTAGCGAGTCTACCGGAATTAATTGGCAACCTTCCTTCACTTGCAAAATTGGGGATTTATTATTGTCCAGAGCTAACTTCACTGCCAAAAGAGATCAGCCACCTCCAGTGTTCAGTACATTTGAATATTTTGCGCACATATCCGCGAGGTAGGCATTTGATTCTATAAATCTTCGTGGGGTATGCAATAGTTCAAATATATCCATGCCAAAAAAAAAAACAAAAAGAGGCACTGTTTACTTTTTATTTCTTTATTTTTTTCCTTTTATCTCTTTGGATGCCTTATTTTCACTATTAATACTTGCCATGATTTGAAGATCCAACATAAAAATCAAAGATTGATTTAGGGAGAGGAATGTCATTAAGTCACTCTTGTTGGAGAAATATTAGGCAATCTAAAACAGAAAATTTTGTAATTTCTCTACATTTCTAAAAATAAATTATAGATTAATTTTTTCTTTTTTAATATAACATGTAGAGGTAGAGATTCAAATTCAAAACTTTTTGGTTGATGATGGTTTTTATAGCTTTTTGTTGGAATGCCTATTAGGAATGAAAAGAAAGTGGAGGCAGTAATAATAATATAATCTTTTTTATCATAATTTAGGAATGAAAAACTATTGAAATTTGACCCTTTTACAACCTTTATTGATTGACTTTCTTTTTCATGGAGTTCAACCACAAATAAACGTTGCAAGATTTGAACTTCGAATCATCTTAGAAAGATTTGAACTTCGGATCTTTAGAGGGTTGTTATGCCTTAACGAAATAAAAATTGTAAAGATATATAATATAGGAAACCACGATATCATAATATCTCAAGATAATGAATAAATGATATAGGTACGAATTGCAATCATTCTGAGTTTGATATCCAAAGAAACCTTATGCATTTGGAAGTTTATGGCAATTGGTGTCTGATTTAAGGTCCATGTGACTCGTTGTCCCTTCACTATTCTGCAATTTAGTGCAAACTTTTGAAAGCTTGGGCAACTACAAATATTTTGCACAAGAAAACCTGAGTTGTTGTTAAGCCTTTCTCTAAATCTGTTCTCTACCAATTTCTCATTTTTCCTCAATGGCAGAATCAATTTTGTTCGATGTTGCGGGGAGCCTTATAATGAAACTTGGCTCTCACGCATTCAGAAGTCTGGGATCCATCTGGGGTCTCAATGATGAGCTTGACAAACTAGAAGACACCATTTCCTCCATTAAAGCCGTACTTCTCGATGCAGAGGAGCAGCAGTCCAAGCGCCACGCGGTGAAGGTTTGGATTTCAAGGCTTAAAGATGCTCTCTACGATGTTGATGACTTGATTGATGAGTTCTCTTACGAAGCCTTAAGGACACAAGTTAAGGCCACGTGTGGAAGAAAGAACGCACAGGTACGCATGTTCTTCTCCAAATCAAATCCTATTGCATTTGGTCTTCGAATGGGTCACAAAATAAAGGCAATTAGGGAGAAATTAAAGGATATTGATGCTGATCAAAAAATAAACTTAAACTTTTTGGTGGCGATGATGAATATGCGAGATGATGGAAATAGGAAGGAACGGGAGACTTACTCTTTTATACCTGAAGGGGAAGTGATTGGTAGGGCTGATGAAAGGAAAGCAATCATAGATCTTTTGTTGGATACCAACTCTAAAGAGAATATTGGAGTGATTGCCATAGTTGGAATTGGAGGATTAGGAAAGACCACACTTGCTCAATTTGTTTATAACGATGAGAAGATATCAAAACATTTTGAGCTGAAACTGTGGGTGTGTGTTTCTGAACAATTTGATGTTAAAATTGTTGCTGAAAAGATTCTAGAGTCTGCTACAAAGAAGAGATGTGAGCCCCTTGAGATGGATCCATTGCAAAGTTCACTTCGAAATGAAATCGACGGTAAAAAATATCTACTCATCATGGATGACGTGTGGAATGAGAATTATGAAAAATGGATTAATTTGAAAAGATTGTTAGTGGGTGGTGCAACTGGGAGTAGGATTTTGATCACCACACGTGGTAAACAAGTTGCTGAAACATTTGATGTGTCTTCGTTATATTCTTTAGAAAATCTAAATGAAGATGATTCTTGGTTATTGTTTACAAAAATGGCATCTTTGGAGGTAGTAGAAAAGGAGCCTGAGAATTCAAATTTTATCAAAATTGGCAAGGAGATTGCGGCGAAGTTAAAAGGTGTTCCTCTTGCCATAAGAATTGTTGGACGACATCTACATTCCAAGAGATCAGAAGTTGATTGGTTGTCTTTCAAGGACAATGAACTTTCAAAGGCTACACATGAGATGCAATCAATACTTAAGATTAGTTATAACCACCTCTCGTCTGATTTAAAGCAGTGTTTTACATATTGTGCTTTATTCCCTAAAGATTATGATATTCAAAAGCATGGAGTGATAAAGTATTGGGTCGCACAAGGTTTCATTCAACCACATAGTACAAAGGCAATCGAAGATGTTGGTGATGATTATTTCATGGAGTTACTATGGAGGTCGTTCTTTCAAGATATAAAAAATGATGAATTTGGAGAGATTAAACAGTACAAGATGCACGACTTCATGCATGATCTTGCAAGTTCAATAGTTGACAAAGAATGTGTTCTTGCAAGTTTGGATGTTGGGACTATTGTAAAAAGAACTCGACATGTGTTCATTGAGTCAAGCTCAAATAAAGTGTTGGAACTTCCGCAACTTTTAACAAAGGCAAAGAATTTGAGAACATTATATTATCCTCCTATTCGTCGTAACAAAACCCTTCACATAATCATTTCTATTCATTTGCAATTACGAATATTGGTTTTGAGGTACACATGAAAGAATTGCCCAAGTCTATTGGCAAGTTGAAACATTTGAGATATCTCGACCTTACTAATAACATGTGACAACTCTTCAAAATTTGAATATAGGCTTATGTCCAAATTTAACGATTCTACCGGAATGGATTGGCAAACTTACATCACTTTCATCATTGTTTATTTGGAAGTGTACAGAGATAACTTCGCTGCCTGAAGGAATTGGCCACCTCGTTTCACTTACACTTTTGGACGTTTCTTGGTGTTCTAATTTAAATTCGCTGCCGGAAGGGATCCGCCATCTCCACTCCTTGCAAGAGATTTTGATGCATGGATGCCCAAAATTGATAAAAGGGTGCAAGAAAGGAAATGGAGAAGACTGGCCTAAAATCGCCCATGTCCCGAGCGTATATCCTCAGCCTTGA

mRNA sequence

ATGGCGGAATCTATTCTCTTCAGCCTTACAGGAAAAATTATAATGAAATTGGGGTCTTTCGCAGGCCAAGAGCTTGCATTGCTCTGGGGCGTGGTTAATGAGCTTGACAAGCTCAAACGCATGCTTTCTGCCATTCAAGCCGTGGTTCTGGATGCAGAGGAGCAGCAGTTCAAGAGCCATGCAGTCAAGGATTGGATTTCAAAGCTTAAAGATGCTCTCTACGACATTGAGGACCTATTTGATGAGCTCTCTTATGCAACCCTGAGAATGCAAGTTATGGCTAGAGATAATAGAAGAAAGACCTACAAACAGTGTACAGAGATAACTTCGCTGCCTGAAGGAATTGGCCACCTCGTTTCACTTACACTTTTGGACGTTTCTTGGTGTTCTAATTTAAATTCGCTGCCGGAAGGGATCCGCCATCTCCACTCCTTGCAAGAGATTTTGATGCATGGATGCCCAAAATTGATAAAAGGGTGCAAGAAAGGAAATGGAGAAGACTGGCCTAAAATCGCCCATGTCCCGAGCGTATATCCTCAGCCTTGA

Coding sequence (CDS)

ATGGCGGAATCTATTCTCTTCAGCCTTACAGGAAAAATTATAATGAAATTGGGGTCTTTCGCAGGCCAAGAGCTTGCATTGCTCTGGGGCGTGGTTAATGAGCTTGACAAGCTCAAACGCATGCTTTCTGCCATTCAAGCCGTGGTTCTGGATGCAGAGGAGCAGCAGTTCAAGAGCCATGCAGTCAAGGATTGGATTTCAAAGCTTAAAGATGCTCTCTACGACATTGAGGACCTATTTGATGAGCTCTCTTATGCAACCCTGAGAATGCAAGTTATGGCTAGAGATAATAGAAGAAAGACCTACAAACAGTGTACAGAGATAACTTCGCTGCCTGAAGGAATTGGCCACCTCGTTTCACTTACACTTTTGGACGTTTCTTGGTGTTCTAATTTAAATTCGCTGCCGGAAGGGATCCGCCATCTCCACTCCTTGCAAGAGATTTTGATGCATGGATGCCCAAAATTGATAAAAGGGTGCAAGAAAGGAAATGGAGAAGACTGGCCTAAAATCGCCCATGTCCCGAGCGTATATCCTCAGCCTTGA

Protein sequence

MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSHAVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQCTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGCKKGNGEDWPKIAHVPSVYPQP

Homology

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BLAST of Moc09g05640.1 vs. NCBI nr
Match: XP_022150756.1 (LOW QUALITY PROTEIN: putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 201.1 bits (510), Expect = 8.4e-48
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH
Sbjct: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 105
           AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ
Sbjct: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 104

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BLAST of Moc09g05640.1 vs. NCBI nr
Match: XP_022154689.1 (putative disease resistance protein RGA3 [Momordica charantia] >XP_022154697.1 putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 139.8 bits (351), Expect = 2.3e-29
Identity = 72/126 (57.14%), Postives = 93/126 (73.81%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MA+SILF++ G +IMKLGS A +ELALLWGV +EL+KLK  +SAI+AV+LDAEEQQ+KSH
Sbjct: 1   MAQSILFNVAGSLIMKLGSLAFRELALLWGVDDELEKLKDAVSAIKAVLLDAEEQQYKSH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQCTEITSLPEGIGHLVS 120
           AVK+W+S+LKD LYD++DL DE SY TLR QV A+D R+          S P   G  + 
Sbjct: 61  AVKNWVSRLKDVLYDVDDLIDEFSYETLRRQVKAKDGRKPEQVCMFFSKSNPVAFGFRMG 120

Query: 121 LTLLDV 127
            T+ D+
Sbjct: 121 QTIKDI 126

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BLAST of Moc09g05640.1 vs. NCBI nr
Match: KAF9829898.1 (hypothetical protein H0E87_023305, partial [Populus deltoides])

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-28
Identity = 66/177 (37.29%), Postives = 107/177 (60.45%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           M+ +   ++   ++ KLGSFAGQE  L WG+  ++ +L++ + AI AV+ DAE++Q K+ 
Sbjct: 1   MSGAFATTIAQSLLGKLGSFAGQEFCLAWGLEADIARLEKRMKAINAVLSDAEQKQSKNE 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQCTEITSLPEGIGHLVS 120
            ++ W++ L + LYD ED+ DE++  TLR QV+          + T  TS     G   S
Sbjct: 61  RIRLWLNDLTEVLYDAEDVLDEIACETLRRQVV----------KTTGSTSRKLLQGSAES 120

Query: 121 LTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGCKKGNGEDWPKIAHVPSV 178
           L +  + +C N+  +P+ I +L+ LQ + +  CP L K C+KG GEDWPK+AH+P +
Sbjct: 121 LQIFIIRYCPNIEEMPDCIGNLNKLQNLEISDCPSLSKRCQKGTGEDWPKVAHIPKI 167

[Close]

BLAST of Moc09g05640.1 vs. NCBI nr
Match: XP_022152874.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 131.7 bits (330), Expect = 6.3e-27
Identity = 71/104 (68.27%), Postives = 79/104 (75.96%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MAESILF L G II  L SFA QELALL GV +ELDKL+  LS IQAV+LDAEEQQ+K H
Sbjct: 1   MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 105
           A K WISKLKDALYDI DLFDE+SY  LR Q  +++NR K   Q
Sbjct: 61  AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQ 104

[Close]

BLAST of Moc09g05640.1 vs. NCBI nr
Match: XP_022152874.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 79.0 bits (193), Expect = 4.8e-11
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 0


Query: 103  KQCTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGCKK 162
            + C  + SLPE I  L SLT L +  C  L SLPEGIR L  L+ + +  CP L + C++
Sbjct: 1177 RDCPNLMSLPEPIEKLTSLTKLYIERCPKLTSLPEGIRQLQCLKSLQIRRCPILNERCER 1236

Query: 163  GNGEDWPKIAHVPSV 178
              GEDW KIAH+P V
Sbjct: 1237 QTGEDWFKIAHIPDV 1251

HSP 2 Score: 131.7 bits (330), Expect = 6.3e-27
Identity = 69/100 (69.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MAE+IL+++T  II KLGS A QEL LLWGV +ELDKLK  LSAIQAV+LDAEEQQ KS 
Sbjct: 1   MAEAILYNVTADIIFKLGSSALQELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSL 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRK 101
           AVK W+S+LKDALY+I+DL DE SY TLR QV+A+D R++
Sbjct: 61  AVKAWVSRLKDALYEIDDLVDESSYETLRRQVLAKDQRKR 100

[Close]

BLAST of Moc09g05640.1 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-15
Identity = 39/74 (52.70%), Postives = 52/74 (70.27%), Query Frame = 0


Query: 105 CTEITSLP-EGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGCKKG 164
           C  + SLP EG+  L SLT L V  C+ L  LPEG++HL +L  + + GCP+LIK C+KG
Sbjct: 893 CCALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKG 952

Query: 165 NGEDWPKIAHVPSV 178
            GEDW KI+H+P+V
Sbjct: 953 IGEDWHKISHIPNV 966

HSP 2 Score: 58.5 bits (140), Expect = 8.8e-08
Identity = 28/70 (40.00%), Postives = 45/70 (64.29%), Query Frame = 0


Query: 13 IIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSHAVKDWISKLKDA 72
          ++  L SF   EL LL+G  +E  +L  M S IQAV+ DA+E+Q  +  +++W+ KL  A
Sbjct: 9  LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68

Query: 73 LYDIEDLFDE 83
           Y+++D+ DE
Sbjct: 69 TYEVDDILDE 78

[Close]

BLAST of Moc09g05640.1 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-15
Identity = 39/76 (51.32%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 103 KQCTEITSLP-EGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGCK 162
           + C  + SLP EG+  L SLT L V  C+ L  LPEG++HL +L  + + GCP+LIK C+
Sbjct: 913 RYCYALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCE 972

Query: 163 KGNGEDWPKIAHVPSV 178
           KG GEDW KI+H+P+V
Sbjct: 973 KGIGEDWHKISHIPNV 988

HSP 2 Score: 58.9 bits (141), Expect = 6.8e-08
Identity = 35/91 (38.46%), Postives = 51/91 (56.04%), Query Frame = 0


Query: 1  MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
          MAE+ L  L    +  L  F   EL L++G   E  KL  M S IQAV+ DA+E+Q K  
Sbjct: 1  MAEAFLQVL----LDNLTFFIQGELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK 60

Query: 61 AVKDWISKLKDALYDIEDLFDELSYATLRMQ 92
          A+K+W+ KL  A Y+++D+ D+      R +
Sbjct: 61 AIKNWLQKLNVAAYEVDDILDDCKTEAARFK 87

[Close]

BLAST of Moc09g05640.1 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 8.2e-14
Identity = 36/81 (44.44%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 98  RRKTYKQCTEITSLPE-GIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKL 157
           +R   + C  + S PE G+  L SLT L V +C  L  LPEG++HL +L  + + GCP++
Sbjct: 905 KRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEV 964

Query: 158 IKGCKKGNGEDWPKIAHVPSV 178
            K C K  GEDW KIAH+P++
Sbjct: 965 EKRCDKEIGEDWHKIAHIPNL 985

HSP 2 Score: 64.7 bits (156), Expect = 1.2e-09
Identity = 34/91 (37.36%), Postives = 54/91 (59.34%), Query Frame = 0


Query: 1  MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
          MAE+ L  L    +  L SF G +L L++G   E +KL  + S IQAV+ DA+E+Q K  
Sbjct: 1  MAEAFLQVL----LENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61 AVKDWISKLKDALYDIEDLFDELSYATLRMQ 92
          A+++W+ KL  A Y+++D+  E     +R +
Sbjct: 61 AIENWLQKLNSAAYEVDDILGECKNEAIRFE 87

[Close]

BLAST of Moc09g05640.1 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-13
Identity = 36/75 (48.00%), Postives = 49/75 (65.33%), Query Frame = 0


Query: 102 YKQCTEITSLP-EGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGC 161
           ++ C  + SLP EG+  L SLT L VS C  L  LPEG++HL +L  + +  CP + K C
Sbjct: 899 FEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCPIVFKRC 958

Query: 162 KKGNGEDWPKIAHVP 176
           ++G GEDW KIAH+P
Sbjct: 959 ERGIGEDWHKIAHIP 973

HSP 2 Score: 58.5 bits (140), Expect = 8.8e-08
Identity = 28/70 (40.00%), Postives = 44/70 (62.86%), Query Frame = 0


Query: 13 IIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSHAVKDWISKLKDA 72
          ++  L SF   EL LL+G  +E  +L  M S IQAV+ DA+E+Q     +++W+ KL  A
Sbjct: 9  VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 73 LYDIEDLFDE 83
           Y+++D+ DE
Sbjct: 69 TYEVDDILDE 78

[Close]

BLAST of Moc09g05640.1 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.7e-06
Identity = 24/72 (33.33%), Postives = 43/72 (59.72%), Query Frame = 0


Query: 33  NELDKLKRMLSAIQAVVLDAEEQQFKSHAVKDWISKLKDALYDIEDLFDELSYATLRMQV 92
           N L++L   L  I AV++DAEE+Q  +  V+ W+++L+D +Y  ED  D+++   LR+ +
Sbjct: 37  NLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI 96

Query: 93  MARDNRRKTYKQ 105
            A  +     +Q
Sbjct: 97  GAESSSSNRLRQ 108

[Close]

BLAST of Moc09g05640.1 vs. ExPASy TrEMBL
Match: A0A6J1DCG4 (LOW QUALITY PROTEIN: putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111018812 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 4.1e-48
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH
Sbjct: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 105
           AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ
Sbjct: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 104

[Close]

BLAST of Moc09g05640.1 vs. ExPASy TrEMBL
Match: A0A6J1DMC1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111021883 PE=4 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.1e-29
Identity = 72/126 (57.14%), Postives = 93/126 (73.81%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MA+SILF++ G +IMKLGS A +ELALLWGV +EL+KLK  +SAI+AV+LDAEEQQ+KSH
Sbjct: 1   MAQSILFNVAGSLIMKLGSLAFRELALLWGVDDELEKLKDAVSAIKAVLLDAEEQQYKSH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQCTEITSLPEGIGHLVS 120
           AVK+W+S+LKD LYD++DL DE SY TLR QV A+D R+          S P   G  + 
Sbjct: 61  AVKNWVSRLKDVLYDVDDLIDEFSYETLRRQVKAKDGRKPEQVCMFFSKSNPVAFGFRMG 120

Query: 121 LTLLDV 127
            T+ D+
Sbjct: 121 QTIKDI 126

[Close]

BLAST of Moc09g05640.1 vs. ExPASy TrEMBL
Match: A0A0A0KBD9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420890 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 3.0e-27
Identity = 69/100 (69.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MAE+IL+++T  II KLGS A QEL LLWGV +ELDKLK  LSAIQAV+LDAEEQQ KS 
Sbjct: 1   MAEAILYNVTADIIFKLGSSALQELGLLWGVNDELDKLKHSLSAIQAVLLDAEEQQSKSL 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRK 101
           AVK W+S+LKDALY+I+DL DE SY TLR QV+A+D R++
Sbjct: 61  AVKAWVSRLKDALYEIDDLVDESSYETLRRQVLAKDQRKR 100

[Close]

BLAST of Moc09g05640.1 vs. ExPASy TrEMBL
Match: A0A6J1DHD4 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020497 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 3.0e-27
Identity = 71/104 (68.27%), Postives = 79/104 (75.96%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MAESILF L G II  L SFA QELALL GV +ELDKL+  LS IQAV+LDAEEQQ+K H
Sbjct: 1   MAESILFDLAGNIITNLASFARQELALLCGVPDELDKLEDXLSDIQAVILDAEEQQYKRH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 105
           A K WISKLKDALYDI DLFDE+SY  LR Q  +++NR K   Q
Sbjct: 61  AXKHWISKLKDALYDIYDLFDEISYEALRRQATSKNNRGKLCNQ 104

[Close]

BLAST of Moc09g05640.1 vs. ExPASy TrEMBL
Match: A0A6J1DHD4 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020497 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.3e-11
Identity = 36/75 (48.00%), Postives = 46/75 (61.33%), Query Frame = 0


Query: 103  KQCTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIKGCKK 162
            + C  + SLPE I  L SLT L +  C  L SLPEGIR L  L+ + +  CP L + C++
Sbjct: 1177 RDCPNLMSLPEPIEKLTSLTKLYIERCPKLTSLPEGIRQLQCLKSLQIRRCPILNERCER 1236

Query: 163  GNGEDWPKIAHVPSV 178
              GEDW KIAH+P V
Sbjct: 1237 QTGEDWFKIAHIPDV 1251

HSP 2 Score: 126.7 bits (317), Expect = 9.7e-26
Identity = 67/104 (64.42%), Postives = 80/104 (76.92%), Query Frame = 0


Query: 1   MAESILFSLTGKIIMKLGSFAGQELALLWGVVNELDKLKRMLSAIQAVVLDAEEQQFKSH 60
           MA+SILF++   +I KLGS A +EL  LWGV +ELDKL+  LSAI+AV+LDAEEQQ KSH
Sbjct: 1   MADSILFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61  AVKDWISKLKDALYDIEDLFDELSYATLRMQVMARDNRRKTYKQ 105
            VKDWISK+KD  YDI+DL DE SY TLR QV+ +D  R T KQ
Sbjct: 61  TVKDWISKIKDVFYDIDDLIDEFSYETLRRQVLTKD--RTTTKQ 102

[Close]

BLAST of Moc09g05640.1 vs. TAIR 10
Match: AT3G25510.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 53.9 bits (128), Expect = 1.5e-07
Identity = 20/56 (35.71%), Postives = 35/56 (62.50%), Query Frame = 0


Query: 103 KQCTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIK 159
           ++C+ +  +P  IGH+ +L  LD+S CS+L  LP  + ++  LQ + +H C  L+K
Sbjct: 861 RKCSSLVEIPTSIGHVTNLWRLDLSGCSSLVELPSSVGNISELQVLNLHNCSNLVK 916

[Close]

BLAST of Moc09g05640.1 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 53.1 bits (126), Expect = 2.6e-07
Identity = 24/72 (33.33%), Postives = 43/72 (59.72%), Query Frame = 0


Query: 33  NELDKLKRMLSAIQAVVLDAEEQQFKSHAVKDWISKLKDALYDIEDLFDELSYATLRMQV 92
           N L++L   L  I AV++DAEE+Q  +  V+ W+++L+D +Y  ED  D+++   LR+ +
Sbjct: 37  NLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNI 96

Query: 93  MARDNRRKTYKQ 105
            A  +     +Q
Sbjct: 97  GAESSSSNRLRQ 108

[Close]

BLAST of Moc09g05640.1 vs. TAIR 10
Match: AT5G11250.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 52.4 bits (124), Expect = 4.5e-07
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 0


Query: 105 CTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKL 157
           C+ +  LP  IG+  +L  +++S CSNL  LP  I +L  LQE+++ GC KL
Sbjct: 852 CSSLLELPSSIGNATNLVYMNLSNCSNLVELPLSIGNLQKLQELILKGCSKL 903

[Close]

BLAST of Moc09g05640.1 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 51.2 bits (121), Expect = 1.0e-06
Identity = 22/54 (40.74%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 105 CTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKLIK 159
           C+ +  LP  IG+L++L  LD+S CS+L  LP  I +L +LQE+ +  C  L++
Sbjct: 870 CSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLINLQELYLSECSSLVE 923

[Close]

BLAST of Moc09g05640.1 vs. TAIR 10
Match: AT2G17060.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 50.4 bits (119), Expect = 1.7e-06
Identity = 25/55 (45.45%), Postives = 34/55 (61.82%), Query Frame = 0


Query: 102 YKQCTEITSLPEGIGHLVSLTLLDVSWCSNLNSLPEGIRHLHSLQEILMHGCPKL 157
           Y Q T I  +P  I +L  L LLD+  C  L SLP+ + +L SLQE+++ GC KL
Sbjct: 777 YLQGTAIKCIPTSIENLQKLILLDLKDCEVLVSLPDCLGNLRSLQELILSGCSKL 831

The following BLAST results are available for this feature:

BLAST of Moc09g05640.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150756.1	8.4e-48	100.00	LOW QUALITY PROTEIN: putative disease resistance protein RGA3 [Momordica charant...	[more]
XP_022154689.1	2.3e-29	57.14	putative disease resistance protein RGA3 [Momordica charantia] >XP_022154697.1 p...	[more]
KAF9829898.1	2.5e-28	37.29	hypothetical protein H0E87_023305, partial [Populus deltoides]	[more]
XP_022152874.1	6.3e-27	68.27	disease resistance protein RGA2-like [Momordica charantia]	[more]
XP_022152874.1	4.8e-11	48.00	disease resistance protein RGA2-like [Momordica charantia]	[more]

BLAST of Moc09g05640.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XBQ9	1.2e-15	52.70	Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ...	[more]
Q7XA40	1.2e-15	51.32	Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA39	8.2e-14	44.44	Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA42	2.4e-13	48.00	Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q9LRR4	3.7e-06	33.33	Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Moc09g05640.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCG4	4.1e-48	100.00	LOW QUALITY PROTEIN: putative disease resistance protein RGA3 OS=Momordica chara...	[more]
A0A6J1DMC1	1.1e-29	57.14	putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A0A0KBD9	3.0e-27	69.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420890 PE=4 SV=1	[more]
A0A6J1DHD4	3.0e-27	68.27	disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1DHD4	2.3e-11	48.00	disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111020...	[more]

BLAST of Moc09g05640.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25510.1	1.5e-07	35.71	disease resistance protein (TIR-NBS-LRR class), putative	[more]
AT3G14470.1	2.6e-07	33.33	NB-ARC domain-containing disease resistance protein	[more]
AT5G11250.1	4.5e-07	42.31	Disease resistance protein (TIR-NBS-LRR class)	[more]
AT1G69550.1	1.0e-06	40.74	disease resistance protein (TIR-NBS-LRR class)	[more]
AT2G17060.1	1.7e-06	45.45	Disease resistance protein (TIR-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 35..55
None	No IPR available	GENE3D	1.20.5.4130		coord: 10..115 e-value: 2.1E-22 score: 81.3
None	No IPR available	PANTHER	PTHR23155:SF909	OS11G0676100 PROTEIN	coord: 4..81
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 107..175
IPR041118	Rx, N-terminal	PFAM	PF18052	Rx_N	coord: 13..96 e-value: 4.3E-26 score: 91.0
IPR044974	Disease resistance protein, plants	PANTHER	PTHR23155	DISEASE RESISTANCE PROTEIN RP	coord: 4..81
IPR038005	Virus X resistance protein-like, coiled-coil domain	CDD	cd14798	RX-CC_like	coord: 2..82 e-value: 4.79441E-12 score: 58.0171

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc09g05640	Moc09g05640	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc09g05640.1.exon1	Moc09g05640.1.exon1	exon
Moc09g05640.1.exon2	Moc09g05640.1.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc09g05640.1	cds.Moc09g05640.1	CDS
cds.Moc09g05640.1	cds.Moc09g05640.1_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc09g05640.1	Moc09g05640.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
molecular_function	GO:0043531	ADP binding