Moc05g30860.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview
NameMoc05g30860.1
TypemRNA
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionpurine-uracil permease NCS1-like
Locationchr5: 23100445 .. 23101956 (+)
Sequence length1512
RNA-Seq ExpressionMoc05g30860.1
SyntenyMoc05g30860.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCGCCGCCGGCCGGCCGACCAACGACCCGCATTTCCGCGATGCCGACTCTGCTCCGACGCTCGGCAGCGAAGATCTCCGGCCTACGACGCCGGAGCAGAGAACGCTCGCCTGGCCGGAGATGGCCACGCTTTGGGTGGGGCTGATCGTCGGCGTCCCGGCTTATTACCTGGCCGGAAGCTTGGTCGACCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTCACCGTAGCGGCGCTGGTTCTCACCGGCCATCCGGGGACCAAATACGGCATATCGTTCCCGATTCTTGCCCGATCGTCGTTCGGAATCCACGGCGCCCACGTCCCCACGCTTCTTCGAGCCCTAATCGGCTGCGGCTGGTACGGAATCGAAACATGGATCGCAGGCGAGGCGATTTTCATTCTATTACCGGAAAGTATCAAGGAATCTTCCTTCTCCCATGTTCTTCCATGGCTGGGAACTTCCCCTCTCGCGTTTTCTTGCTTCTTGGCGTTCTGGGTCGTTCAATTGGCCAGCGTTTGGAAAGGAATGGACGGAATTCGAAAACTGGAAACGTATTCTGCCCCAATTCTTGTTTTCCTCACGTTTGGGCTCTTGATTTGGTCTTATGTTAGGGCCGGTGGATTAGGGCATATGCTGTCTTTAACCTCGAAATTGTCTGCGTCGGAATTTTGGGCTTTGTTTTTCCCCTCTCTTACTGCTAACATAAGCTTTTGGGCGACTCTGGCTTTGAATATCCCCGATTTCACTCGCTACGCTAGAACCCAAAAGGACCAAATCCTCGGCCAATTAGGGCTTCCGATTTTCATGGGGGCGTTCACTTTCGTCGGCGTCGCCGTCACCTCCTCCACCGGGGTCATTTTCGGCCGGGTGATTTCCAACCCGATTGACCTTCTCGGCCACATCGGCGGATTCACCACCAAGGTTTTGGCGATTTTTGGGATCATCCTGGCCACCGTGACCACCAACGTCGCCGCCAATGTGGTGGCGCCGGCGAACGCGCTGGTGAATCTCAGCCCTTCTGTGTTCAGTTTCACGAGAGGCGCCGTGCTCACCGCCGTGGTCGGCATCGTCTTCCAGCCGTGGAGGCTTCTGAGCTCCAGCGAGAGCTTCGTTTACACTTGGCTTGTTGGGTACTCGGCGCTTTTGGGTCCGATCGGCGGGATAATGCTGACGGATTACTATCTGATCCGCCGGAGAGAATTGAGCGTCGAAGACTTGTATTCGTCGAGTCCTTCGGGGGCTTATTACTACTCCGGCGGGTTCAATTTGGCGGCGATGGCGGCGCTGGTCGTCGGAGTGTTGCCGGTGATTCCTGGGTTTTTGGAGAAGGTGGAGATCGTGTCTAGGGTTCCGAAAGTATTCGGAATTATTTACGGAAACGCCTGGTTTATAAGCTCCTTTTTGGCTGGTTTTTGCTATTGGGGTCTCTCTGTTCTGTTGAAACGGCAAAAAGTTCCTCGCCCTTTGGACCCACTTCTTTGA

mRNA sequence

ATGGCCTCCGCCGCCGGCCGGCCGACCAACGACCCGCATTTCCGCGATGCCGACTCTGCTCCGACGCTCGGCAGCGAAGATCTCCGGCCTACGACGCCGGAGCAGAGAACGCTCGCCTGGCCGGAGATGGCCACGCTTTGGGTGGGGCTGATCGTCGGCGTCCCGGCTTATTACCTGGCCGGAAGCTTGGTCGACCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTCACCGTAGCGGCGCTGGTTCTCACCGGCCATCCGGGGACCAAATACGGCATATCGTTCCCGATTCTTGCCCGATCGTCGTTCGGAATCCACGGCGCCCACGTCCCCACGCTTCTTCGAGCCCTAATCGGCTGCGGCTGGTACGGAATCGAAACATGGATCGCAGGCGAGGCGATTTTCATTCTATTACCGGAAAGTATCAAGGAATCTTCCTTCTCCCATGTTCTTCCATGGCTGGGAACTTCCCCTCTCGCGTTTTCTTGCTTCTTGGCGTTCTGGGTCGTTCAATTGGCCAGCGTTTGGAAAGGAATGGACGGAATTCGAAAACTGGAAACGTATTCTGCCCCAATTCTTGTTTTCCTCACGTTTGGGCTCTTGATTTGGTCTTATGTTAGGGCCGGTGGATTAGGGCATATGCTGTCTTTAACCTCGAAATTGTCTGCGTCGGAATTTTGGGCTTTGTTTTTCCCCTCTCTTACTGCTAACATAAGCTTTTGGGCGACTCTGGCTTTGAATATCCCCGATTTCACTCGCTACGCTAGAACCCAAAAGGACCAAATCCTCGGCCAATTAGGGCTTCCGATTTTCATGGGGGCGTTCACTTTCGTCGGCGTCGCCGTCACCTCCTCCACCGGGGTCATTTTCGGCCGGGTGATTTCCAACCCGATTGACCTTCTCGGCCACATCGGCGGATTCACCACCAAGGTTTTGGCGATTTTTGGGATCATCCTGGCCACCGTGACCACCAACGTCGCCGCCAATGTGGTGGCGCCGGCGAACGCGCTGGTGAATCTCAGCCCTTCTGTGTTCAGTTTCACGAGAGGCGCCGTGCTCACCGCCGTGGTCGGCATCGTCTTCCAGCCGTGGAGGCTTCTGAGCTCCAGCGAGAGCTTCGTTTACACTTGGCTTGTTGGGTACTCGGCGCTTTTGGGTCCGATCGGCGGGATAATGCTGACGGATTACTATCTGATCCGCCGGAGAGAATTGAGCGTCGAAGACTTGTATTCGTCGAGTCCTTCGGGGGCTTATTACTACTCCGGCGGGTTCAATTTGGCGGCGATGGCGGCGCTGGTCGTCGGAGTGTTGCCGGTGATTCCTGGGTTTTTGGAGAAGGTGGAGATCGTGTCTAGGGTTCCGAAAGTATTCGGAATTATTTACGGAAACGCCTGGTTTATAAGCTCCTTTTTGGCTGGTTTTTGCTATTGGGGTCTCTCTGTTCTGTTGAAACGGCAAAAAGTTCCTCGCCCTTTGGACCCACTTCTTTGA

Coding sequence (CDS)

ATGGCCTCCGCCGCCGGCCGGCCGACCAACGACCCGCATTTCCGCGATGCCGACTCTGCTCCGACGCTCGGCAGCGAAGATCTCCGGCCTACGACGCCGGAGCAGAGAACGCTCGCCTGGCCGGAGATGGCCACGCTTTGGGTGGGGCTGATCGTCGGCGTCCCGGCTTATTACCTGGCCGGAAGCTTGGTCGACCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTCACCGTAGCGGCGCTGGTTCTCACCGGCCATCCGGGGACCAAATACGGCATATCGTTCCCGATTCTTGCCCGATCGTCGTTCGGAATCCACGGCGCCCACGTCCCCACGCTTCTTCGAGCCCTAATCGGCTGCGGCTGGTACGGAATCGAAACATGGATCGCAGGCGAGGCGATTTTCATTCTATTACCGGAAAGTATCAAGGAATCTTCCTTCTCCCATGTTCTTCCATGGCTGGGAACTTCCCCTCTCGCGTTTTCTTGCTTCTTGGCGTTCTGGGTCGTTCAATTGGCCAGCGTTTGGAAAGGAATGGACGGAATTCGAAAACTGGAAACGTATTCTGCCCCAATTCTTGTTTTCCTCACGTTTGGGCTCTTGATTTGGTCTTATGTTAGGGCCGGTGGATTAGGGCATATGCTGTCTTTAACCTCGAAATTGTCTGCGTCGGAATTTTGGGCTTTGTTTTTCCCCTCTCTTACTGCTAACATAAGCTTTTGGGCGACTCTGGCTTTGAATATCCCCGATTTCACTCGCTACGCTAGAACCCAAAAGGACCAAATCCTCGGCCAATTAGGGCTTCCGATTTTCATGGGGGCGTTCACTTTCGTCGGCGTCGCCGTCACCTCCTCCACCGGGGTCATTTTCGGCCGGGTGATTTCCAACCCGATTGACCTTCTCGGCCACATCGGCGGATTCACCACCAAGGTTTTGGCGATTTTTGGGATCATCCTGGCCACCGTGACCACCAACGTCGCCGCCAATGTGGTGGCGCCGGCGAACGCGCTGGTGAATCTCAGCCCTTCTGTGTTCAGTTTCACGAGAGGCGCCGTGCTCACCGCCGTGGTCGGCATCGTCTTCCAGCCGTGGAGGCTTCTGAGCTCCAGCGAGAGCTTCGTTTACACTTGGCTTGTTGGGTACTCGGCGCTTTTGGGTCCGATCGGCGGGATAATGCTGACGGATTACTATCTGATCCGCCGGAGAGAATTGAGCGTCGAAGACTTGTATTCGTCGAGTCCTTCGGGGGCTTATTACTACTCCGGCGGGTTCAATTTGGCGGCGATGGCGGCGCTGGTCGTCGGAGTGTTGCCGGTGATTCCTGGGTTTTTGGAGAAGGTGGAGATCGTGTCTAGGGTTCCGAAAGTATTCGGAATTATTTACGGAAACGCCTGGTTTATAAGCTCCTTTTTGGCTGGTTTTTGCTATTGGGGTCTCTCTGTTCTGTTGAAACGGCAAAAAGTTCCTCGCCCTTTGGACCCACTTCTTTGA

Protein sequence

MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSSPSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVLLKRQKVPRPLDPLL
Homology
BLAST of Moc05g30860.1 vs. NCBI nr
Match: KAG6596517.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 873.6 bits (2256), Expect = 8.1e-250
Identity = 435/503 (86.48%), Postives = 469/503 (93.24%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASAA  PT++ H  ++      GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLA
Sbjct: 69  MASAADNPTDNTHVENS------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLA 128

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 129 GSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTL 188

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 189 LRALIGCGWYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLA 248

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPS
Sbjct: 249 SVWKGMDGIRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPS 308

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +
Sbjct: 309 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHI 368

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+L
Sbjct: 369 ISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALL 428

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IRRREL VEDLYSSS
Sbjct: 429 TAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSS 488

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYYYSGGFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFIS+F+AG
Sbjct: 489 PTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAG 548

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+KV   L P L
Sbjct: 549 FCYWGLSVLLKRRKVSSLLGPQL 565

BLAST of Moc05g30860.1 vs. NCBI nr
Match: XP_023005190.1 (purine-uracil permease NCS1-like [Cucurbita maxima])

HSP 1 Score: 873.6 bits (2256), Expect = 8.1e-250
Identity = 435/503 (86.48%), Postives = 468/503 (93.04%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASAA  PT++ H  +       GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLA
Sbjct: 69  MASAANNPTDNTHVENP------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLA 128

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 129 GSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTL 188

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 189 LRALIGCGWYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLA 248

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPS
Sbjct: 249 SVWKGMDGIRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPS 308

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +
Sbjct: 309 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHI 368

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+L
Sbjct: 369 ISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALL 428

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY +R+REL VEDLYSSS
Sbjct: 429 TAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSS 488

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYYYSGGFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFISSF+AG
Sbjct: 489 PTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAG 548

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+KV   L P L
Sbjct: 549 FCYWGLSVLLKRRKVSSCLGPQL 565

BLAST of Moc05g30860.1 vs. NCBI nr
Match: XP_038906223.1 (purine-uracil permease NCS1 [Benincasa hispida])

HSP 1 Score: 872.1 bits (2252), Expect = 2.4e-249
Identity = 434/503 (86.28%), Postives = 471/503 (93.64%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASA   P+ D    D+      G EDLRPTTPEQRTL+WPEMA LWVGLIVGVPAYYLA
Sbjct: 62  MASAVDHPSEDTQNEDS------GREDLRPTTPEQRTLSWPEMALLWVGLIVGVPAYYLA 121

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGM+WWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 122 GSLVDLGMSWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTL 181

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK+SSFS VLPWLGTSPLAF CFLAFW+VQLA
Sbjct: 182 LRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFICFLAFWIVQLA 241

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLE +SAPILVFLT  LLIWS VRAGGLGHMLSLTSKLS+SEFWALFFPS
Sbjct: 242 SVWKGMDGIRKLEIFSAPILVFLTVCLLIWSCVRAGGLGHMLSLTSKLSSSEFWALFFPS 301

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTG+IFG +
Sbjct: 302 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGLIFGHI 361

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPI+LLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+L
Sbjct: 362 ISNPIELLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALL 421

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GI+FQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIRRRELSVEDLYS+S
Sbjct: 422 TAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTS 481

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYYY+GGFNLAA+AALVVGVLPVIPGFLEKV+IVS+VPKVF +IYGNAWFIS+FLAG
Sbjct: 482 PTGAYYYTGGFNLAAVAALVVGVLPVIPGFLEKVKIVSKVPKVFSVIYGNAWFISTFLAG 541

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLS+LLKR+K+ R   PLL
Sbjct: 542 FCYWGLSILLKRRKLSRRFGPLL 558

BLAST of Moc05g30860.1 vs. NCBI nr
Match: XP_023539092.1 (purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871.3 bits (2250), Expect = 4.0e-249
Identity = 435/503 (86.48%), Postives = 467/503 (92.84%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASAA  PT++ H  ++      GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLA
Sbjct: 69  MASAADNPTDNTHVENS------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLA 128

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFPI ARSSFGIHGAH+PTL
Sbjct: 129 GSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPIFARSSFGIHGAHIPTL 188

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 189 LRALIGCGWYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLA 248

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPS
Sbjct: 249 SVWKGMDGIRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPS 308

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +
Sbjct: 309 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHI 368

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+L
Sbjct: 369 ISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALL 428

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IRRREL VEDLYS S
Sbjct: 429 TAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSLS 488

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYYYSGGFNL A+ ALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFIS+F+AG
Sbjct: 489 PTGAYYYSGGFNLTAVTALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAG 548

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+KV   L P L
Sbjct: 549 FCYWGLSVLLKRRKVSSCLGPQL 565

BLAST of Moc05g30860.1 vs. NCBI nr
Match: KAG7028053.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 868.2 bits (2242), Expect = 3.4e-248
Identity = 433/503 (86.08%), Postives = 467/503 (92.84%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASAA  PT++ H  ++      GS+DLRPTT EQRTLAWPEMA LW+GL+VGVPAYYLA
Sbjct: 58  MASAADNPTDNTHVENS------GSDDLRPTTLEQRTLAWPEMALLWIGLVVGVPAYYLA 117

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+  RSSFGIHGAH+PTL
Sbjct: 118 GSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFVRSSFGIHGAHIPTL 177

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 178 LRALIGCGWYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLA 237

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPS
Sbjct: 238 SVWKGMDGIRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPS 297

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +
Sbjct: 298 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHI 357

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+L
Sbjct: 358 ISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALL 417

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IRRREL VEDLYSSS
Sbjct: 418 TAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSS 477

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYYYSGGFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFIS+F+AG
Sbjct: 478 PTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAG 537

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+KV   L P L
Sbjct: 538 FCYWGLSVLLKRRKVSSLLGPQL 554

BLAST of Moc05g30860.1 vs. ExPASy Swiss-Prot
Match: Q9LZD0 (Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 3.8e-202
Identity = 338/494 (68.42%), Postives = 412/494 (83.40%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           +++  G   ND  + ++   P+L ++DL+PTTP QRT +W +M++LW+GL+VGVP YYLA
Sbjct: 95  LSAMTGSEINDHGYDESQFDPSLTNDDLKPTTPSQRTFSWLDMSSLWIGLVVGVPTYYLA 154

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVV AN + +  LVLT  PGT YGISFP+LARSSFGI GAH+PTL
Sbjct: 155 GSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGTLYGISFPVLARSSFGIRGAHIPTL 214

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRAL+GCGWYGIETWI GEAIF+LLP  IK+S+ SH LPWLGTSPL FSCF+ FW+ QL 
Sbjct: 215 LRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSHTLPWLGTSPLEFSCFIVFWLAQLC 274

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
            VW+GMDGIRKLE YSAPIL+ LT  LL WSY++AGG GHMLSL+SKL++++FW LFFPS
Sbjct: 275 IVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAGGFGHMLSLSSKLTSAQFWTLFFPS 334

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDF+R+A++Q DQI+GQ+GLP+FMG FTFVGVAVTSST +IFGRV
Sbjct: 335 LTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLPVFMGLFTFVGVAVTSSTSIIFGRV 394

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPI+LLG IGG  T +LAI GI LAT+TTN+AANVVAPANALVNL+P  F+F RGA L
Sbjct: 395 ISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVVAPANALVNLNPKFFTFGRGAFL 454

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLL SSESFVYTWL+GYSALLGPIGGI+L DYYLI++ +L++ DLYS S
Sbjct: 455 TAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGIILVDYYLIKKMKLNIGDLYSLS 514

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           PSG YY+S G+N+AA+ ALV G++PV+PGFL K+  +S++   F ++Y NA F S  +AG
Sbjct: 515 PSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALSKISNGFVVVYDNALFFSFIIAG 574

Query: 481 FCYWGLSVLLKRQK 495
           F YW +   L R++
Sbjct: 575 FVYWIIMSRLGRKQ 588

BLAST of Moc05g30860.1 vs. ExPASy Swiss-Prot
Match: P94575 (Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 3.7e-80
Identity = 180/482 (37.34%), Postives = 268/482 (55.60%), Query Frame = 0

Query: 23  LGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANA 82
           L +EDL P   E+RT      A++W+G I  +P Y   G L+ +G++ WQ +A ++ A+ 
Sbjct: 13  LSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIIITASL 72

Query: 83  VTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIF 142
           +   AL L GH GTKYG+ FP++ R+S+GI+GA++P LLRA     W GI+T+    A+ 
Sbjct: 73  ILFGALALNGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGSTALN 132

Query: 143 ILLPESIKE-SSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILV 202
           ILL                LG        F+ FW + L  +  GM+ I++ E ++ P LV
Sbjct: 133 ILLLNMWPGWGEIGGEWNILGIHLSGLLSFVFFWAIHLLVLHHGMESIKRFEVWAGP-LV 192

Query: 203 FLTFGLLIWSYVR-AGGLGHMLSLTSKLS--ASEFWALFFPSLTANISFWATLALNIPDF 262
           +L FG ++W  V  AGGLG + S   K    +  FW  F   +T  I  WATL LNIPDF
Sbjct: 193 YLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWP-FAAGVTGIIGIWATLILNIPDF 252

Query: 263 TRYARTQKDQILGQL-GLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTK 322
           TR+A TQK+QI GQ  GLP     F F  + VTS + V FG  I + +D+L         
Sbjct: 253 TRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVI 312

Query: 323 VLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPWRLLSSS 382
           VL++  + +AT++ NVAAN+V+PA  + N  P   +F RG+ +TA++ +   PW+L+ S+
Sbjct: 313 VLSVITLCIATISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESA 372

Query: 383 ESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSSPSGAYYYSGGFNLAAMA 442
            S VY +L     +LGP+ G+M+ DY++IR+RELSV+DLYS   +G Y Y  G+N  A A
Sbjct: 373 TS-VYAFLGLIGGMLGPVAGVMMADYFIIRKRELSVDDLYSE--TGRYVYWKGYNYRAFA 432

Query: 443 ALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAGFCYWGLSVLLKRQKVPR 500
           A ++G L         + ++     V   +Y  +WF+   ++   Y    ++L R   P 
Sbjct: 433 ATMLGAL---------ISLIGMYVPVLKSLYDISWFVGVLISFLFY----IVLMRVHPPA 476

BLAST of Moc05g30860.1 vs. ExPASy Swiss-Prot
Match: O14035 (Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29B12.14c PE=3 SV=2)

HSP 1 Score: 234.6 bits (597), Expect = 2.5e-60
Identity = 155/452 (34.29%), Postives = 235/452 (51.99%), Query Frame = 0

Query: 23  LGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANA 82
           L +EDL P    QR          W+   V V  + +AG+ V+ G++WW+   TV     
Sbjct: 48  LTNEDLYPVPKSQRVWGPWNYVAFWLADSVNVNTWMIAGTAVESGLSWWEAWITVWVGYT 107

Query: 83  VTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIF 142
           +    L + G  G  Y ISFP+L+RSSFGI G+  P L RA++ C WYG++ WI GE + 
Sbjct: 108 IAAFILTIAGRAGAVYHISFPVLSRSSFGIWGSLWPILNRAVMACVWYGVQAWIGGECVT 167

Query: 143 ILLPESIKESSFSHV---LPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPI 202
           +++  SI   SFSH+   +   GT    +  F  FW++   ++W  +  IR L T  + +
Sbjct: 168 LMI-RSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLISNVAIWFPVYQIRHLFTAKSFL 227

Query: 203 LVFLTFGLLIWSYVRAGGLGHMLSLTSKLSA-SEFWALFFPSLTANISFWATLALNIPDF 262
                   LIW+ V+A G G  +   ++LS  +  WA+    + + +  +ATL +N PDF
Sbjct: 228 APPAAIAFLIWALVKAHGAGDAIHAKTQLSTWNHGWAV-TAGIISCLDNFATLIVNNPDF 287

Query: 263 TRYARTQKDQILGQL-GLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLL-------GH 322
           TR+A T    I  QL  +P+  G  T +GV V S++  I+G  I NP+DLL        H
Sbjct: 288 TRFATTPNAPIFPQLITIPMGFGITTLIGVLVGSASKSIYGENIWNPLDLLKSFLDHSNH 347

Query: 323 IGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQP 382
            G          G+ LA +  N+AAN V+  N    L P   +  RG  + +++GI   P
Sbjct: 348 HGVRAGVFFISTGLCLAQLGVNIAANTVSAGNDTSALCPMFINIRRGGYIASIIGICMCP 407

Query: 383 WRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSSPSGAYYYSGG 442
           W LLSSS SF  + L  Y+  L    GI++ DY++IR+  L V+ LY+ +P+  Y+++ G
Sbjct: 408 WNLLSSSNSFANS-LSAYAVFLSSFAGILIADYFVIRKGYLKVDALYTINPNEPYWFTYG 467

Query: 443 FNLAAMAALVVGVLPVIPGFLEKVEIVSRVPK 463
            NL A A+ + G+L  + G    V    +VPK
Sbjct: 468 INLRAFASYICGLLINVVGLAGAVG--DKVPK 493

BLAST of Moc05g30860.1 vs. ExPASy Swiss-Prot
Match: Q10279 (Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fur4 PE=1 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 8.0e-59
Identity = 149/468 (31.84%), Postives = 239/468 (51.07%), Query Frame = 0

Query: 23  LGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANA 82
           L ++DL P  P +R        + W+   V +  + ++ + ++LG+ WW+    V     
Sbjct: 41  LTNKDLYPVPPSKRLWGPWNFISFWLADAVNINTWMISATAIELGLNWWEAWICVWVGYL 100

Query: 83  VTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLRALIGCGWYGIETWIAGEAIF 142
           +    +  TG PG  Y ISFP+L+RSSFG  G+  P L R+++ C WYG++ WI GE + 
Sbjct: 101 ICGILVATTGRPGAVYHISFPVLSRSSFGTWGSLWPILNRSVLACVWYGVQAWIGGECV- 160

Query: 143 ILLPESIKESSFSHV---LPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPI 202
           +L+  SI   SFSH+   +   GT    +  F  FW++   ++W  +  IR L T+ A +
Sbjct: 161 VLMIRSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLLSNIAIWFPVHQIRHLFTFKAIV 220

Query: 203 LVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPSLTANISFWATLALNIPDFT 262
                   LIW+ V+A G G ++   +KL   E   +    +   I  +ATL +N PDF 
Sbjct: 221 APPAAIAFLIWALVKAHGAGPVIHEPTKLGQYEHAWVVINGIVTCIDGFATLIVNNPDFA 280

Query: 263 RYARTQKDQILGQ-LGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLL------GHIG 322
           R+A T       Q + +P+  G  + +GV V+S++  I+G  + +P  LL       +  
Sbjct: 281 RFATTPGAVHWPQIITVPLAFGVTSLIGVLVSSASKAIYGTTLWDPTQLLASFLDHSNAH 340

Query: 323 GFTTKVLAI-FGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPW 382
           G    V  I FG+ +A +  N+AAN V+  N L  L P+V +  RG  + +++ +   PW
Sbjct: 341 GVRAGVFFIAFGLCIAQLGVNIAANSVSAGNDLSALLPTVINVRRGGYIASIIALCMCPW 400

Query: 383 RLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSSPSGAYYYSGGF 442
            LLSS+ +F  T+L  YS  L    G+++ DYY +R+  + V  LYSSS S  YY+  G 
Sbjct: 401 NLLSSNNNFT-TYLSSYSVFLSSFAGVIIADYYFVRKGLIRVAPLYSSSSSSPYYFWKGI 460

Query: 443 NLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLA 480
           N  A A+ + G+L  I G         +VPKV   ++   +F+   +A
Sbjct: 461 NFRAFASYICGMLINIVGMAGSTG--QKVPKVANTMFNLNYFLGITVA 503

BLAST of Moc05g30860.1 vs. ExPASy Swiss-Prot
Match: P75712 (Putative allantoin permease OS=Escherichia coli (strain K12) OX=83333 GN=ybbW PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 4.1e-47
Identity = 124/449 (27.62%), Postives = 230/449 (51.22%), Query Frame = 0

Query: 25  SEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVT 84
           SEDL P T  QRT       TLW+G +  VP Y + G    LG++ +  +  ++ +    
Sbjct: 14  SEDLLPKTQSQRTWKTFNYFTLWMGSVHNVPNYVMVGGFFILGLSTFSIMLAIILSAFFI 73

Query: 85  VAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTLLR-ALIGCGWYGIETWIAGEAIFI 144
            A +VL G  G+KYG+ F ++ R+S+G+ GA  P LLR  +    W+G++ +    A  I
Sbjct: 74  AAVMVLNGAAGSKYGVPFAMILRASYGVRGALFPGLLRGGIAAIMWFGLQCYAGSLACLI 133

Query: 145 LLPESIKE-SSFSHVLPWLGTSPLAFSCFLAFWVVQLASVWKGMDGIRKLETYSAPILVF 204
           L+ +      +       LG S      FL FW+V +   + G   + K      P +  
Sbjct: 134 LIGKIWPGFLTLGGDFTLLGLSLPGLITFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYI 193

Query: 205 LTFGLLIWSYVRAGGLGHMLS-LTSKLSASEFWA-LFFPSLTANISFWATLALNIPDFTR 264
           +  G+ IW+ +   G+G +   + S +  +E    LF   + A ++ WA  A++  DFT+
Sbjct: 194 VFGGMAIWA-ISLVGIGPIFDYIPSGIQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQ 253

Query: 265 YARTQKDQILGQ-LGLPIFMGAFTFVGVAVTSSTGVIFGRVISNPIDLLGHIGGFTTKVL 324
            A + ++Q LGQ LGL +    F   GV + +   + +G    N +D++           
Sbjct: 254 NAHSFREQALGQTLGLVVAYILFAVAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFF 313

Query: 325 AIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVLTAVVGIVFQPWRLLSSSES 384
           A+  I++ T++TN   N++     +  ++P+  ++  G ++ +++ ++  PW+L+ + +S
Sbjct: 314 AVLVILMTTISTNATGNIIPAGYQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDS 373

Query: 385 FVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSSPSGAYYYSGGFNLAAMAAL 444
            +Y +L     +LGP+ G+M+  Y+++ R ++++++LY ++P    YY  GFNL A +  
Sbjct: 374 -IYLFLDIIGGMLGPVIGVMMAHYFVVMRGQINLDELY-TAPGDYKYYDNGFNLTAFSVT 433

Query: 445 VVGVLPVIPG-FLEKVEIVSRVPKVFGII 468
           +V V+  + G F+  +E +SRV    G+I
Sbjct: 434 LVAVILSLGGKFIHFMEPLSRVSWFVGVI 459

BLAST of Moc05g30860.1 vs. ExPASy TrEMBL
Match: A0A6J1KWR9 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 3.9e-250
Identity = 435/503 (86.48%), Postives = 468/503 (93.04%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASAA  PT++ H  +       GS+DLRPTTPEQRTLAWPEMA LW+GL+VGVPAYYLA
Sbjct: 69  MASAANNPTDNTHVENP------GSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLA 128

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 129 GSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTL 188

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 189 LRALIGCGWYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLA 248

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPS
Sbjct: 249 SVWKGMDGIRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPS 308

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +
Sbjct: 309 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHI 368

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+L
Sbjct: 369 ISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALL 428

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY +R+REL VEDLYSSS
Sbjct: 429 TAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSS 488

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYYYSGGFNL A+AALVVGVLPVIPGFLEKV+IVS+VP+VF +IYGNAWFISSF+AG
Sbjct: 489 PTGAYYYSGGFNLTAVAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAG 548

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+KV   L P L
Sbjct: 549 FCYWGLSVLLKRRKVSSCLGPQL 565

BLAST of Moc05g30860.1 vs. ExPASy TrEMBL
Match: A0A6J1GDF7 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 9.0e-247
Identity = 430/503 (85.49%), Postives = 467/503 (92.84%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASAA  PT++ H  ++      GS+DLRPT PEQRTLAWPEMA LW+GL+VGVPAYYLA
Sbjct: 69  MASAADNPTDNTHVENS------GSDDLRPTMPEQRTLAWPEMALLWIGLVVGVPAYYLA 128

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVVAANAVT+ AL+LTGHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 129 GSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTL 188

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK SSFS VLPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 189 LRALIGCGWYGIETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLA 248

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET SAPILVFLT GLLIWS VRAGG GHMLSL SKLS+SEFWALFFPS
Sbjct: 249 SVWKGMDGIRKLETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPS 308

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLP+FMGAFTFVGVAVTSSTGVIFG +
Sbjct: 309 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHI 368

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNL+PS F+FTRGA+L
Sbjct: 369 ISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALL 428

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+ DYY IRRREL VEDLYSSS
Sbjct: 429 TAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSS 488

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+GAYY+SGGFNL A+AALVVGVLPVIPGFLEKV++VS+VP++F +IYGNAWFIS+F+AG
Sbjct: 489 PTGAYYFSGGFNLTAVAALVVGVLPVIPGFLEKVKLVSKVPQLFTVIYGNAWFISTFIAG 548

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCY GLSVLLKR+KV   L P L
Sbjct: 549 FCYLGLSVLLKRRKVSSWLGPQL 565

BLAST of Moc05g30860.1 vs. ExPASy TrEMBL
Match: A0A5D3DPI8 (Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003170 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.9e-245
Identity = 428/503 (85.09%), Postives = 463/503 (92.05%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASA   P+ D    D+      G +DLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLA
Sbjct: 58  MASADANPSEDTQNDDS------GKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLA 117

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGM+WWQGIATVV ANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 118 GSLVDLGMSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTL 177

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK+SSFS +LPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 178 LRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLA 237

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS+SEFWALFFPS
Sbjct: 238 SVWKGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPS 297

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +
Sbjct: 298 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHI 357

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+L
Sbjct: 358 ISNPIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALL 417

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GI+FQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+S
Sbjct: 418 TAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTS 477

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P GAYYYSGGFNL A+ ALVVGVLPVIPGFLEKVEIVS+VPKVF +IYG+AWFISSFLAG
Sbjct: 478 PRGAYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAG 537

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+K      PLL
Sbjct: 538 FCYWGLSVLLKRRKASLRFGPLL 554

BLAST of Moc05g30860.1 vs. ExPASy TrEMBL
Match: A0A1S3B756 (purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 2.9e-245
Identity = 428/503 (85.09%), Postives = 463/503 (92.05%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASA   P+ D    D+      G +DLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLA
Sbjct: 58  MASADANPSEDTQNDDS------GKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLA 117

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGM+WWQGIATVV ANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 118 GSLVDLGMSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTL 177

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAIF+LLPESIK+SSFS +LPWLGTSPLAF+CFLAFWVVQLA
Sbjct: 178 LRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLA 237

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS+SEFWALFFPS
Sbjct: 238 SVWKGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPS 297

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +
Sbjct: 298 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHI 357

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLLGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+L
Sbjct: 358 ISNPIDLLGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALL 417

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GI+FQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+S
Sbjct: 418 TAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTS 477

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P GAYYYSGGFNL A+ ALVVGVLPVIPGFLEKVEIVS+VPKVF +IYG+AWFISSFLAG
Sbjct: 478 PRGAYYYSGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAG 537

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+K      PLL
Sbjct: 538 FCYWGLSVLLKRRKASLRFGPLL 554

BLAST of Moc05g30860.1 vs. ExPASy TrEMBL
Match: A0A0A0LAP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.7e-241
Identity = 419/503 (83.30%), Postives = 457/503 (90.85%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           MASA   P  D    D+      G EDLRPTT EQRTL+WPEMA LW+GLIVGVP+YYLA
Sbjct: 58  MASATANPPEDTQNEDS------GKEDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLA 117

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGM+WWQGIATVVAANAVT+ AL+L GHPGTKYGISFP+ ARSSFGIHGAH+PTL
Sbjct: 118 GSLVDLGMSWWQGIATVVAANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTL 177

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRALIGCGWYGIETWIAGEAI +LLP+SIK+S+FS  LPWLGTSPLAF+CFL FWV+QLA
Sbjct: 178 LRALIGCGWYGIETWIAGEAISLLLPQSIKKSAFSQTLPWLGTSPLAFTCFLGFWVIQLA 237

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
           SVWKGMDGIRKLET+SAPILVFLT  LLIWS V+AGG GHMLSLTSKLS SEFWALFFPS
Sbjct: 238 SVWKGMDGIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSFSEFWALFFPS 297

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDFTRYA++QKDQI+GQLGLPIFMGAFTFVGVAVTSSTGVIFG +
Sbjct: 298 LTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHI 357

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPIDLL HIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPS F+FTRGA+L
Sbjct: 358 ISNPIDLLDHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALL 417

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GI+FQPWRLLSSSESFVYTWLVGYSALLGPIGG+M+TDYYLIR +ELSVEDLYS+S
Sbjct: 418 TAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMMTDYYLIRGKELSVEDLYSTS 477

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           P+ AYYYSGGFNL A+ ALVVGVLPVIPGFLEKVE+VS+VPKVF +IYG+AWF+S+FLAG
Sbjct: 478 PTAAYYYSGGFNLVAVVALVVGVLPVIPGFLEKVELVSKVPKVFSVIYGSAWFVSTFLAG 537

Query: 481 FCYWGLSVLLKRQKVPRPLDPLL 504
           FCYWGLSVLLKR+K      P L
Sbjct: 538 FCYWGLSVLLKRRKASLRFGPPL 554

BLAST of Moc05g30860.1 vs. TAIR 10
Match: AT5G03555.1 (permease, cytosine/purines, uracil, thiamine, allantoin family protein )

HSP 1 Score: 705.7 bits (1820), Expect = 2.7e-203
Identity = 338/494 (68.42%), Postives = 412/494 (83.40%), Query Frame = 0

Query: 1   MASAAGRPTNDPHFRDADSAPTLGSEDLRPTTPEQRTLAWPEMATLWVGLIVGVPAYYLA 60
           +++  G   ND  + ++   P+L ++DL+PTTP QRT +W +M++LW+GL+VGVP YYLA
Sbjct: 95  LSAMTGSEINDHGYDESQFDPSLTNDDLKPTTPSQRTFSWLDMSSLWIGLVVGVPTYYLA 154

Query: 61  GSLVDLGMAWWQGIATVVAANAVTVAALVLTGHPGTKYGISFPILARSSFGIHGAHVPTL 120
           GSLVDLGMAWWQGIATVV AN + +  LVLT  PGT YGISFP+LARSSFGI GAH+PTL
Sbjct: 155 GSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGTLYGISFPVLARSSFGIRGAHIPTL 214

Query: 121 LRALIGCGWYGIETWIAGEAIFILLPESIKESSFSHVLPWLGTSPLAFSCFLAFWVVQLA 180
           LRAL+GCGWYGIETWI GEAIF+LLP  IK+S+ SH LPWLGTSPL FSCF+ FW+ QL 
Sbjct: 215 LRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSHTLPWLGTSPLEFSCFIVFWLAQLC 274

Query: 181 SVWKGMDGIRKLETYSAPILVFLTFGLLIWSYVRAGGLGHMLSLTSKLSASEFWALFFPS 240
            VW+GMDGIRKLE YSAPIL+ LT  LL WSY++AGG GHMLSL+SKL++++FW LFFPS
Sbjct: 275 IVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAGGFGHMLSLSSKLTSAQFWTLFFPS 334

Query: 241 LTANISFWATLALNIPDFTRYARTQKDQILGQLGLPIFMGAFTFVGVAVTSSTGVIFGRV 300
           LTANISFWATLALNIPDF+R+A++Q DQI+GQ+GLP+FMG FTFVGVAVTSST +IFGRV
Sbjct: 335 LTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLPVFMGLFTFVGVAVTSSTSIIFGRV 394

Query: 301 ISNPIDLLGHIGGFTTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSVFSFTRGAVL 360
           ISNPI+LLG IGG  T +LAI GI LAT+TTN+AANVVAPANALVNL+P  F+F RGA L
Sbjct: 395 ISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVVAPANALVNLNPKFFTFGRGAFL 454

Query: 361 TAVVGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGIMLTDYYLIRRRELSVEDLYSSS 420
           TAV+GIVFQPWRLL SSESFVYTWL+GYSALLGPIGGI+L DYYLI++ +L++ DLYS S
Sbjct: 455 TAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGIILVDYYLIKKMKLNIGDLYSLS 514

Query: 421 PSGAYYYSGGFNLAAMAALVVGVLPVIPGFLEKVEIVSRVPKVFGIIYGNAWFISSFLAG 480
           PSG YY+S G+N+AA+ ALV G++PV+PGFL K+  +S++   F ++Y NA F S  +AG
Sbjct: 515 PSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALSKISNGFVVVYDNALFFSFIIAG 574

Query: 481 FCYWGLSVLLKRQK 495
           F YW +   L R++
Sbjct: 575 FVYWIIMSRLGRKQ 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6596517.18.1e-25086.48Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023005190.18.1e-25086.48purine-uracil permease NCS1-like [Cucurbita maxima][more]
XP_038906223.12.4e-24986.28purine-uracil permease NCS1 [Benincasa hispida][more]
XP_023539092.14.0e-24986.48purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo][more]
KAG7028053.13.4e-24886.08Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
Q9LZD03.8e-20268.42Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1[more]
P945753.7e-8037.34Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI ... [more]
O140352.5e-6034.29Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / A... [more]
Q102798.0e-5931.84Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812... [more]
P757124.1e-4727.62Putative allantoin permease OS=Escherichia coli (strain K12) OX=83333 GN=ybbW PE... [more]
Match NameE-valueIdentityDescription
A0A6J1KWR93.9e-25086.48purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=... [more]
A0A6J1GDF79.0e-24785.49purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 P... [more]
A0A5D3DPI82.9e-24585.09Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B7562.9e-24585.09purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1[more]
A0A0A0LAP39.7e-24183.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G03555.12.7e-20368.42permease, cytosine/purines, uracil, thiamine, allantoin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.4160.10Hydantoin permeasecoord: 15..480
e-value: 1.0E-116
score: 392.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR30618:SF0PURINE-URACIL PERMEASE NCS1coord: 15..493
NoneNo IPR availablePANTHERPTHR30618NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTERcoord: 15..493
NoneNo IPR availableCDDcd11485SLC-NCS1sbd_YbbW-likecoord: 21..487
e-value: 0.0
score: 521.744
IPR001248Purine-cytosine permeasePFAMPF02133Transp_cyt_purcoord: 31..467
e-value: 5.9E-103
score: 345.0

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Moc05g30860Moc05g30860gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Moc05g30860.1.exon1Moc05g30860.1.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Moc05g30860.1cds.Moc05g30860.1CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Moc05g30860.1Moc05g30860.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0015851 nucleobase transport
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity
molecular_function GO:0015205 nucleobase transmembrane transporter activity