Moc03g22650.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g22650.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ty3-gypsy retrotransposon protein
Location	chr3: 16005890 .. 16006686 (+)
Sequence length	606
RNA-Seq Expression	Moc03g22650.1
Synteny	Moc03g22650.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTACGAATGCAACTGGAGGAACGTTGCGACGCTACGGAACAAGAGGTAACTAATCTGAAGGAGATGCTCACCAGAGTGAAGGAGCAGGTTCAGATTAACCAGAAGATCCTTGAAACTTACATGGCCAAGATGGATGCACGGCACAAAAGGATTGAGCGCTTCATGGCGCACGGCTCCGTCGAAAAGTCATCAATGATTGAATACAGTCGAGGAGAAAGATCATCGGTAAAAAGGAAGGCGACAATGAAGAAACTCGTATCCTGGAGACTACGGAGACCGAAACTGACTGTAGCAAGTTCGAAAAAGTTGAGATGTCGATTTTCTTGGGCCAGACCTTGAATCGTTGCTATTCCGTGCCGAGTGGTATTTCGAAATACATAAACTAACTGATTCGGAAAAACCAATAGTGTCTGTGATAAGCTTTGACGGAACAACTTTAGCTTGGTATCGGTGGAATGACCATCGATGATGGTTTCTGGATTGGAACGACTTGAAGAAGCGACTATTCCAACGTTTTCTACCCTCTAAACGGGGAAGATAATGTGCCAAAATTATTCGCGATAAAGCAGGAAACCACCGTTGAAGACTATATCGAACGGTTTAAGGCTATGGCAGCGCCGTTACCACAACTCTCCGACGAAGTCCTCGAAGGCGTTTTCCTCAACGGGCTGAAATCGGTGATTAGGGCTGAAGTTTTGGCCATGGAACCTGATGGGTTAGATCAAATTATGCGAAAAGCCCAGTTAGTGGAAGATATTGACCTGGTGTCCAAGGAGATGGAGGGACGGTCATAG

mRNA sequence

ATGGTACGAATGCAACTGGAGGAACGTTGCGACGCTACGGAACAAGAGGTAACTAATCTGAAGGAGATGCTCACCAGAGTGAAGGAGCAGGTTCAGATTAACCAGAAGATCCTTGAAACTTACATGGCCAAGATGGATGCACGGCACAAAAGGATTGAGCGCTTCATGGCGCACGGCTCCGTCGAAAAGTCATCAATGATTGAATACAGTCGAGGAGAAAGATCATCGGTAAAAAGGAAGGCGACAATGAAGAAACTCGTATCCTGGAGACTACGGAGACCGAAACTGACTGTAGCAAGTTCGAAAAAAAGCGACTATTCCAACGTTTTCTACCCTCTAAACGGGGAAGATAATGTGCCAAAATTATTCGCGATAAAGCAGGAAACCACCGTTGAAGACTATATCGAACGGTTTAAGGCTATGGCAGCGCCGTTACCACAACTCTCCGACGAAGTCCTCGAAGGCGTTTTCCTCAACGGGCTGAAATCGGTGATTAGGGCTGAAGTTTTGGCCATGGAACCTGATGGGTTAGATCAAATTATGCGAAAAGCCCAGTTAGTGGAAGATATTGACCTGGTGTCCAAGGAGATGGAGGGACGGTCATAG

Coding sequence (CDS)

ATGGTACGAATGCAACTGGAGGAACGTTGCGACGCTACGGAACAAGAGGTAACTAATCTGAAGGAGATGCTCACCAGAGTGAAGGAGCAGGTTCAGATTAACCAGAAGATCCTTGAAACTTACATGGCCAAGATGGATGCACGGCACAAAAGGATTGAGCGCTTCATGGCGCACGGCTCCGTCGAAAAGTCATCAATGATTGAATACAGTCGAGGAGAAAGATCATCGGTAAAAAGGAAGGCGACAATGAAGAAACTCGTATCCTGGAGACTACGGAGACCGAAACTGACTGTAGCAAGTTCGAAAAAAAGCGACTATTCCAACGTTTTCTACCCTCTAAACGGGGAAGATAATGTGCCAAAATTATTCGCGATAAAGCAGGAAACCACCGTTGAAGACTATATCGAACGGTTTAAGGCTATGGCAGCGCCGTTACCACAACTCTCCGACGAAGTCCTCGAAGGCGTTTTCCTCAACGGGCTGAAATCGGTGATTAGGGCTGAAGTTTTGGCCATGGAACCTGATGGGTTAGATCAAATTATGCGAAAAGCCCAGTTAGTGGAAGATATTGACCTGGTGTCCAAGGAGATGGAGGGACGGTCATAG

Protein sequence

MVRMQLEERCDATEQEVTNLKEMLTRVKEQVQINQKILETYMAKMDARHKRIERFMAHGSVEKSSMIEYSRGERSSVKRKATMKKLVSWRLRRPKLTVASSKKSDYSNVFYPLNGEDNVPKLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQIMRKAQLVEDIDLVSKEMEGRS

Homology

[Close]

BLAST of Moc03g22650.1 vs. NCBI nr
Match: XP_022148929.1 (uncharacterized protein LOC111017476 [Momordica charantia])

HSP 1 Score: 98.2 bits (243), Expect = 8.5e-17
Identity = 77/221 (34.84%), Postives = 116/221 (52.49%), Query Frame = 0


Query: 1   MVRMQLEERCDATEQEVTNLKEMLT--RVKEQVQINQKI---LETYMAKMDARHKRIERF 60
           M+  +LE RC A E+EVT++K  +T  +V+EQ  ++++I   LE++M+++          
Sbjct: 1   MMVKELESRCGAMEKEVTDIKGAITVVKVEEQRVMSERIQNMLESFMSEIIGGKTPSISQ 60

Query: 61  MAHGSVEKSSMIE------------YSRGERSSVKR--------KATMKKLVSWRLRRPK 120
           +    V+K+ + E            Y R +   V+                  W   R +
Sbjct: 61  LGESLVQKNKLTEEATRVVQINDQAYDRNKFKKVEMPIFIVISFDVVALAWYRWHEPRDQ 120

Query: 121 LTVASSKKSDYSNVFYPLNGEDNVPKLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEG 180
            T   + ++     F          +L AIKQE +V +Y E F+A++APLPQLSDEVLE 
Sbjct: 121 FTDWDNLRTRSLKRFRKSKEGRQCARLLAIKQEGSVAEYQEAFEALSAPLPQLSDEVLEC 180

Query: 181 VFLNGLKSVIRAEVLAMEPDGLDQIMRKAQLVEDIDLVSKE 197
            FLNGL  V+R++VLA EP GL+QIM +AQLVEDI    +E
Sbjct: 181 TFLNGLDLVVRSKVLATEPQGLEQIMHRAQLVEDIATAVQE 221

[Close]

BLAST of Moc03g22650.1 vs. NCBI nr
Match: XP_022131713.1 (uncharacterized protein LOC111004816 [Momordica charantia])

HSP 1 Score: 85.1 bits (209), Expect = 7.5e-13
Identity = 43/73 (58.90%), Postives = 56/73 (76.71%), Query Frame = 0


Query: 124 AIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQIMRK 183
           AIKQE +VE+Y E F+A++A  P L +EVLE  +LNGL  ++RAEVLA +P GLDQIMR+
Sbjct: 2   AIKQEGSVEEYQEAFEALSATQPHLVEEVLESDYLNGLNPILRAEVLASKPTGLDQIMRR 61

Query: 184 AQLVEDIDLVSKE 197
           AQL+ED    S+E
Sbjct: 62  AQLIEDQATTSQE 74

[Close]

BLAST of Moc03g22650.1 vs. NCBI nr
Match: XP_031745472.1 (uncharacterized protein K02A2.6-like [Cucumis sativus])

HSP 1 Score: 79.3 bits (194), Expect = 4.1e-11
Identity = 57/196 (29.08%), Postives = 100/196 (51.02%), Query Frame = 0


Query: 1   MVRMQLEERCDATEQEVTNLKEMLTRVKEQV-QINQKILETYMAKMDARHKRIERFMAHG 60
           M + Q+EER + TE+E+ +LKEM+  +K+ + ++  ++ E + AK     K+ E      
Sbjct: 1   MAQRQIEERVEGTEKEIMSLKEMMLEMKKSMDRMTDELRENHGAK-----KKEEAGTTTT 60

Query: 61  SVEKSSMIEYSRGERSSVKRKATMKKLVSWRLRRPKLTVASSKKSDYSNVFYPLNGEDNV 120
           S EK  +   S G+               W  RR ++      K    + F     +   
Sbjct: 61  SAEKVKVAVVSFGQEE--------VDWFRWSNRRKRVESWEDLKERMFDFFNDTGQQSLG 120

Query: 121 PKLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQ 180
            +L  IKQE +  +Y+++F   +APLP +++ VL   FL GL+  ++AEV++  P  L++
Sbjct: 121 ARLIRIKQEGSYSEYVKKFVTYSAPLPDMAESVLLDAFLTGLEPSLQAEVISRHPQTLEE 180

Query: 181 IMRKAQLVEDIDLVSK 196
            M++AQLV D ++  K
Sbjct: 181 CMKEAQLVNDRNIALK 183

[Close]

BLAST of Moc03g22650.1 vs. NCBI nr
Match: KAA0047599.1 (ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa] >TYK08256.1 ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 78.2 bits (191), Expect = 9.1e-11
Identity = 35/69 (50.72%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 121 KLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQI 180
           +  AIKQE TVE+Y++RF+ ++APLP+++++VLEG F NGL  +IR EV +M   GL+ +
Sbjct: 16  RFLAIKQEGTVEEYLQRFEELSAPLPEIAEDVLEGTFTNGLDPIIRIEVFSMRALGLEDM 75

Query: 181 MRKAQLVED 190
           M  AQL E+
Sbjct: 76  MEAAQLAEE 84

[Close]

BLAST of Moc03g22650.1 vs. NCBI nr
Match: XP_022848903.1 (uncharacterized protein LOC111371244 [Olea europaea var. sylvestris])

HSP 1 Score: 76.6 bits (187), Expect = 2.7e-10
Identity = 55/168 (32.74%), Postives = 84/168 (50.00%), Query Frame = 0


Query: 44  KMDARHKRIERFMAHG--------SVEKSSMI----EYSRGERSSVKRKATMKKLVSWRL 103
           +MD R +R+E  + HG          E+   I     + + E ++V  +        W  
Sbjct: 94  RMDGRSRRLEMPVFHGENPDGWLFKAERYFSINHFSNWEKIEAAAVCFEGEALAWYQWED 153

Query: 104 RRPKLTVASSKKSDYSNVFYPLNGEDNVPKLFAIKQETTVEDYIERFKAMAAPLPQLSDE 163
            R         K+     F P     N+ K  A++QE TV DY   F+ +AAPL  + ++
Sbjct: 154 GRQTFQRWEDLKAALLLRFRPSQEGTNLEKFLALRQEGTVRDYGRMFEVLAAPLTTVPED 213

Query: 164 VLEGVFLNGLKSVIRAEVLAMEPDGLDQIMRKAQLVEDIDLVSKEMEG 200
           +LEG F+NGLK +IRAEV  ++P GLD IM  AQ VED + + ++ +G
Sbjct: 214 ILEGNFINGLKPLIRAEVRLLKPRGLDLIMELAQRVEDRNELVRQAQG 261

[Close]

BLAST of Moc03g22650.1 vs. ExPASy TrEMBL
Match: A0A6J1D5H9 (uncharacterized protein LOC111017476 OS=Momordica charantia OX=3673 GN=LOC111017476 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.1e-17
Identity = 77/221 (34.84%), Postives = 116/221 (52.49%), Query Frame = 0


Query: 1   MVRMQLEERCDATEQEVTNLKEMLT--RVKEQVQINQKI---LETYMAKMDARHKRIERF 60
           M+  +LE RC A E+EVT++K  +T  +V+EQ  ++++I   LE++M+++          
Sbjct: 1   MMVKELESRCGAMEKEVTDIKGAITVVKVEEQRVMSERIQNMLESFMSEIIGGKTPSISQ 60

Query: 61  MAHGSVEKSSMIE------------YSRGERSSVKR--------KATMKKLVSWRLRRPK 120
           +    V+K+ + E            Y R +   V+                  W   R +
Sbjct: 61  LGESLVQKNKLTEEATRVVQINDQAYDRNKFKKVEMPIFIVISFDVVALAWYRWHEPRDQ 120

Query: 121 LTVASSKKSDYSNVFYPLNGEDNVPKLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEG 180
            T   + ++     F          +L AIKQE +V +Y E F+A++APLPQLSDEVLE 
Sbjct: 121 FTDWDNLRTRSLKRFRKSKEGRQCARLLAIKQEGSVAEYQEAFEALSAPLPQLSDEVLEC 180

Query: 181 VFLNGLKSVIRAEVLAMEPDGLDQIMRKAQLVEDIDLVSKE 197
            FLNGL  V+R++VLA EP GL+QIM +AQLVEDI    +E
Sbjct: 181 TFLNGLDLVVRSKVLATEPQGLEQIMHRAQLVEDIATAVQE 221

[Close]

BLAST of Moc03g22650.1 vs. ExPASy TrEMBL
Match: A0A6J1BU87 (uncharacterized protein LOC111004816 OS=Momordica charantia OX=3673 GN=LOC111004816 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 3.6e-13
Identity = 43/73 (58.90%), Postives = 56/73 (76.71%), Query Frame = 0


Query: 124 AIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQIMRK 183
           AIKQE +VE+Y E F+A++A  P L +EVLE  +LNGL  ++RAEVLA +P GLDQIMR+
Sbjct: 2   AIKQEGSVEEYQEAFEALSATQPHLVEEVLESDYLNGLNPILRAEVLASKPTGLDQIMRR 61

Query: 184 AQLVEDIDLVSKE 197
           AQL+ED    S+E
Sbjct: 62  AQLIEDQATTSQE 74

[Close]

BLAST of Moc03g22650.1 vs. ExPASy TrEMBL
Match: A0A5D3CD29 (Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G00260 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.4e-11
Identity = 35/69 (50.72%), Postives = 52/69 (75.36%), Query Frame = 0


Query: 121 KLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQI 180
           +  AIKQE TVE+Y++RF+ ++APLP+++++VLEG F NGL  +IR EV +M   GL+ +
Sbjct: 16  RFLAIKQEGTVEEYLQRFEELSAPLPEIAEDVLEGTFTNGLDPIIRIEVFSMRALGLEDM 75

Query: 181 MRKAQLVED 190
           M  AQL E+
Sbjct: 76  MEAAQLAEE 84

[Close]

BLAST of Moc03g22650.1 vs. ExPASy TrEMBL
Match: A0A5D3BUF1 (Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1418G00090 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.9e-10
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 0


Query: 89  WRLRRPKLTVASSKKSDYSNVFYPLNGEDNVPKLFAIKQETTVEDYIERFKAMAAPLPQL 148
           W   R +       K    N F     E    +  AIKQE +V +++++F+ ++APLP++
Sbjct: 110 WTENRKRFRSWKELKERMYNRFQSREHETTCARFLAIKQEGSVSEHLQKFEELSAPLPEM 169

Query: 149 SDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQIMRKAQLVEDIDLVSKEME 199
            +EVLEG F NGL  +I  EV +M   GL+ +M  AQL E   LV +++E
Sbjct: 170 VEEVLEGTFANGLDPIILKEVFSMRVVGLEDMMEVAQLAEVAQLVEEKVE 219

[Close]

BLAST of Moc03g22650.1 vs. ExPASy TrEMBL
Match: A0A5A7SLB5 (Gypsy/ty3 element polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46346G00030 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 3.7e-10
Identity = 39/71 (54.93%), Postives = 51/71 (71.83%), Query Frame = 0


Query: 131 VEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQIMRKAQLVEDI 190
           V D+ ERF+A++APL  LSDEVLE  FLNGL   I+AEVL+ EP GL QIMR A  +ED 
Sbjct: 143 VADFRERFEALSAPLSHLSDEVLESTFLNGLDPEIKAEVLSYEPVGLGQIMRAALWIEDK 202

Query: 191 DLVSKEMEGRS 202
            +V++   G++
Sbjct: 203 QMVTRSRLGQT 213

[Close]

BLAST of Moc03g22650.1 vs. TAIR 10
Match: AT3G42723.1 (aminoacyl-tRNA ligases;ATP binding;nucleotide binding )

HSP 1 Score: 47.4 bits (111), Expect = 1.6e-05
Identity = 25/70 (35.71%), Postives = 42/70 (60.00%), Query Frame = 0


Query: 120 PKLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQ 179
           P    I+QE +V +Y ERF+A+      L  + LE +FL GL+  ++  V  ++P+G+ Q
Sbjct: 341 PHYSGIQQEGSVREYRERFEALCLGSVILPGQGLEALFLQGLQPSLQTAVRELKPNGIVQ 400

Query: 180 IMRKAQLVED 190
           +M  AQ +E+
Sbjct: 401 MMDTAQWLEE 410

[Close]

BLAST of Moc03g22650.1 vs. TAIR 10
Match: AT3G29750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 42.4 bits (98), Expect = 5.2e-04
Identity = 22/79 (27.85%), Postives = 42/79 (53.16%), Query Frame = 0


Query: 120 PKLFAIKQETTVEDYIERFKAMAAPLPQLSDEVLEGVFLNGLKSVIRAEVLAMEPDGLDQ 179
           P    I+QE +V DY ERF+A+      L  +  E +FL GL+  ++  V  ++P+G++ 
Sbjct: 7   PHYSGIQQEGSVRDYRERFEALCLRSVTLPGQGFEEMFLQGLQPSLQTAVRELKPNGINS 66

Query: 180 IMRKAQLVEDIDLVSKEME 199
              +   +  + LV  +++
Sbjct: 67  YQSRQAELMSLTLVQAKLD 85

The following BLAST results are available for this feature:

BLAST of Moc03g22650.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148929.1	8.5e-17	34.84	uncharacterized protein LOC111017476 [Momordica charantia]	[more]
XP_022131713.1	7.5e-13	58.90	uncharacterized protein LOC111004816 [Momordica charantia]	[more]
XP_031745472.1	4.1e-11	29.08	uncharacterized protein K02A2.6-like [Cucumis sativus]	[more]
KAA0047599.1	9.1e-11	50.72	ty3-gypsy retrotransposon protein [Cucumis melo var. makuwa] >TYK08256.1 ty3-gyp...	[more]
XP_022848903.1	2.7e-10	32.74	uncharacterized protein LOC111371244 [Olea europaea var. sylvestris]	[more]

BLAST of Moc03g22650.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc03g22650.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5H9	4.1e-17	34.84	uncharacterized protein LOC111017476 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1BU87	3.6e-13	58.90	uncharacterized protein LOC111004816 OS=Momordica charantia OX=3673 GN=LOC111004...	[more]
A0A5D3CD29	4.4e-11	50.72	Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A5D3BUF1	2.9e-10	36.36	Ty3-gypsy retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A5A7SLB5	3.7e-10	54.93	Gypsy/ty3 element polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]

BLAST of Moc03g22650.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G42723.1	1.6e-05	35.71	aminoacyl-tRNA ligases;ATP binding;nucleotide binding	[more]
AT3G29750.1	5.2e-04	27.85	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 3..30

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc03g22650	Moc03g22650	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc03g22650.1.exon1	Moc03g22650.1.exon1	exon
Moc03g22650.1.exon2	Moc03g22650.1.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc03g22650.1	cds.Moc03g22650.1	CDS
cds.Moc03g22650.1	cds.Moc03g22650.1_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc03g22650.1	Moc03g22650.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0003676	nucleic acid binding