Moc02g05540.1 (mRNA) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g05540.1
Type	mRNA
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RING/U-box superfamily protein
Location	chr2: 4049253 .. 4049795 (+)
Sequence length	270
RNA-Seq Expression	Moc02g05540.1
Synteny	Moc02g05540.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGTCGATCCAACCCACGGCAGCGGATACCGCCGAGGCGCCAGCTGTTGCCGCCGCGACGCCATATTTTGCAGCTGGAATCGGAAAGGAGGAGGAGCAGGCAGTAGTGGCTGAAGCTCCTGAAATCCGACGGCTGGGAAGGCATCATTTTCACACCTCCATGGCCGGTGGAGGGGTGCTGATCGGCGGCCTCGCCACTGCTGTATTTGCTGTGGTCTTTTGCTACATTCGGGTGACTAGAAAACCAAACGCTGTCAATTAAGCTTTAGTTTTTTTTTTTCAACTTTGTTTACAGGTCAAGAAAGGATTGGTATGTTAGTTAAGCTATACTGGGGCTTTCTATTACCCTTTTTCGGCCCTTCTGTGGAATTGATTGATTACTTTTTGTCCATGGAGAATGCAGAAGAATAAACCTCTTTAAATTAACTGATTCGACACTGCCAGAGAAAACAGAACAGTGGACAGAAAGGTCTGTGTTCTGTTTCTGATCATCAAGTTTTGGCAGGATTTCGTCAATATTGTGTTTGCTGCGATATTTTAG

mRNA sequence

ATGACGTCGATCCAACCCACGGCAGCGGATACCGCCGAGGCGCCAGCTGTTGCCGCCGCGACGCCATATTTTGCAGCTGGAATCGGAAAGGAGGAGGAGCAGGCAGTAGTGGCTGAAGCTCCTGAAATCCGACGGCTGGGAAGGCATCATTTTCACACCTCCATGGCCGGTGGAGGGGTGCTGATCGGCGGCCTCGCCACTGCTGTATTTGCTGTGGTCTTTTGCTACATTCGGGATTTCGTCAATATTGTGTTTGCTGCGATATTTTAG

Coding sequence (CDS)

ATGACGTCGATCCAACCCACGGCAGCGGATACCGCCGAGGCGCCAGCTGTTGCCGCCGCGACGCCATATTTTGCAGCTGGAATCGGAAAGGAGGAGGAGCAGGCAGTAGTGGCTGAAGCTCCTGAAATCCGACGGCTGGGAAGGCATCATTTTCACACCTCCATGGCCGGTGGAGGGGTGCTGATCGGCGGCCTCGCCACTGCTGTATTTGCTGTGGTCTTTTGCTACATTCGGGATTTCGTCAATATTGTGTTTGCTGCGATATTTTAG

Protein sequence

MTSIQPTAADTAEAPAVAAATPYFAAGIGKEEEQAVVAEAPEIRRLGRHHFHTSMAGGGVLIGGLATAVFAVVFCYIRDFVNIVFAAIF

Homology

[Close]

BLAST of Moc02g05540.1 vs. NCBI nr
Match: XP_023532519.1 (uncharacterized protein LOC111794654 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 101.3 bits (251), Expect = 4.5e-18
Identity = 57/77 (74.03%), Postives = 60/77 (77.92%), Query Frame = 0


Query: 5   QPTAADTAEAPAVAAATPYFAAGIGKE---EEQAVVAEAPEIRRLGRHHFHTSMAGGGVL 64
           Q TAA TAEAP VA A P+F A IG E   E Q  VA  PEIRRLG+HH HTSMAGGGVL
Sbjct: 33  QLTAASTAEAPTVATAMPFFGAEIGMEMEMENQQGVAAGPEIRRLGKHHLHTSMAGGGVL 92

Query: 65  IGGLATAVFAVVFCYIR 79
           +GGLATAVFAVVFCYIR
Sbjct: 93  LGGLATAVFAVVFCYIR 109

[Close]

BLAST of Moc02g05540.1 vs. NCBI nr
Match: XP_022947872.1 (uncharacterized protein LOC111451632 [Cucurbita moschata] >KAG6604835.1 hypothetical protein SDJN03_02152, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 98.2 bits (243), Expect = 3.8e-17
Identity = 57/77 (74.03%), Postives = 60/77 (77.92%), Query Frame = 0


Query: 5  QPTAADTAEAPAVAAATPYFAAGIGKE---EEQAVVAEAPEIRRLGRHHFHTSMAGGGVL 64
          Q TAA TAEAP VA A P+F   IGKE   E Q  VA  PEIRRLG+HH HTSMAGGGVL
Sbjct: 20 QLTAASTAEAPTVATAMPFFGE-IGKEVEMENQPGVAAGPEIRRLGKHHLHTSMAGGGVL 79

Query: 65 IGGLATAVFAVVFCYIR 79
          +GGLATAVFAVVFCYIR
Sbjct: 80 LGGLATAVFAVVFCYIR 95

[Close]

BLAST of Moc02g05540.1 vs. NCBI nr
Match: KAG7034949.1 (hypothetical protein SDJN02_01742 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 98.2 bits (243), Expect = 3.8e-17
Identity = 57/77 (74.03%), Postives = 60/77 (77.92%), Query Frame = 0


Query: 5   QPTAADTAEAPAVAAATPYFAAGIGKE---EEQAVVAEAPEIRRLGRHHFHTSMAGGGVL 64
           Q TAA TAEAP VA A P+F   IGKE   E Q  VA  PEIRRLG+HH HTSMAGGGVL
Sbjct: 65  QLTAASTAEAPTVATAMPFFGE-IGKEVEMENQPGVAAGPEIRRLGKHHLHTSMAGGGVL 124

Query: 65  IGGLATAVFAVVFCYIR 79
           +GGLATAVFAVVFCYIR
Sbjct: 125 LGGLATAVFAVVFCYIR 140

[Close]

BLAST of Moc02g05540.1 vs. NCBI nr
Match: KGN46704.1 (hypothetical protein Csa_020980 [Cucumis sativus])

HSP 1 Score: 87.8 bits (216), Expect = 5.1e-14
Identity = 53/80 (66.25%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 1  MTSIQPTAADTAEAPAVAAATPYFAAGIGKEE--EQAVVAEAPEIRRLGRHHFHTSMAGG 60
          M  IQ TAA+  EAP V  A  +F + IGKEE  +  VVAE P IRRLG+HHFHTS+AGG
Sbjct: 17 MFFIQLTAANVKEAPMVDTAL-FFGSKIGKEEAVQGPVVAEGPAIRRLGKHHFHTSVAGG 76

Query: 61 GVLIGGLATAVFAVVFCYIR 79
           VLIG LATAVFA+VFCYIR
Sbjct: 77 RVLIGSLATAVFAIVFCYIR 95

[Close]

BLAST of Moc02g05540.1 vs. NCBI nr
Match: KAE8645753.1 (hypothetical protein Csa_020263 [Cucumis sativus])

HSP 1 Score: 75.1 bits (183), Expect = 3.4e-10
Identity = 37/48 (77.08%), Postives = 41/48 (85.42%), Query Frame = 0


Query: 33 EQAVVAEAPEIRRLGRHHFHTSMAGGGVLIGGLATAVFAVVFCYIRDF 81
          E + VAEAPEIRRLG+HH   SMAGGGV+IGGL TAVFA VFCYIR+F
Sbjct: 46 EGSEVAEAPEIRRLGKHHGDKSMAGGGVIIGGLVTAVFAAVFCYIRNF 93

[Close]

BLAST of Moc02g05540.1 vs. ExPASy TrEMBL
Match: A0A6J1G7L7 (uncharacterized protein LOC111451632 OS=Cucurbita moschata OX=3662 GN=LOC111451632 PE=4 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.8e-17
Identity = 57/77 (74.03%), Postives = 60/77 (77.92%), Query Frame = 0


Query: 5  QPTAADTAEAPAVAAATPYFAAGIGKE---EEQAVVAEAPEIRRLGRHHFHTSMAGGGVL 64
          Q TAA TAEAP VA A P+F   IGKE   E Q  VA  PEIRRLG+HH HTSMAGGGVL
Sbjct: 20 QLTAASTAEAPTVATAMPFFGE-IGKEVEMENQPGVAAGPEIRRLGKHHLHTSMAGGGVL 79

Query: 65 IGGLATAVFAVVFCYIR 79
          +GGLATAVFAVVFCYIR
Sbjct: 80 LGGLATAVFAVVFCYIR 95

[Close]

BLAST of Moc02g05540.1 vs. ExPASy TrEMBL
Match: A0A0A0KCY0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124190 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.5e-14
Identity = 53/80 (66.25%), Postives = 60/80 (75.00%), Query Frame = 0


Query: 1  MTSIQPTAADTAEAPAVAAATPYFAAGIGKEE--EQAVVAEAPEIRRLGRHHFHTSMAGG 60
          M  IQ TAA+  EAP V  A  +F + IGKEE  +  VVAE P IRRLG+HHFHTS+AGG
Sbjct: 17 MFFIQLTAANVKEAPMVDTAL-FFGSKIGKEEAVQGPVVAEGPAIRRLGKHHFHTSVAGG 76

Query: 61 GVLIGGLATAVFAVVFCYIR 79
           VLIG LATAVFA+VFCYIR
Sbjct: 77 RVLIGSLATAVFAIVFCYIR 95

[Close]

BLAST of Moc02g05540.1 vs. ExPASy TrEMBL
Match: A0A200QMD0 (Uncharacterized protein OS=Macleaya cordata OX=56857 GN=BVC80_8997g5 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.8e-10
Identity = 37/53 (69.81%), Postives = 44/53 (83.02%), Query Frame = 0


Query: 30  KEEEQAVVAEAPEIRRLGRHHFHT----SMAGGGVLIGGLATAVFAVVFCYIR 79
           K E+Q  VAEAPEIRR+G+HH H+    S+AGGGV+IGGLATA+FA VFCYIR
Sbjct: 69  KTEDQDEVAEAPEIRRMGKHHHHSSTDESIAGGGVIIGGLATAIFAAVFCYIR 121

[Close]

BLAST of Moc02g05540.1 vs. ExPASy TrEMBL
Match: A0A0A0K3N0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G045480 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.1e-09
Identity = 36/46 (78.26%), Postives = 39/46 (84.78%), Query Frame = 0


Query: 33 EQAVVAEAPEIRRLGRHHFHTSMAGGGVLIGGLATAVFAVVFCYIR 79
          E + VAEAPEIRRLG+HH   SMAGGGV+IGGL TAVFA VFCYIR
Sbjct: 46 EGSEVAEAPEIRRLGKHHGDKSMAGGGVIIGGLVTAVFAAVFCYIR 91

[Close]

BLAST of Moc02g05540.1 vs. ExPASy TrEMBL
Match: A0A2N9IXN3 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56997 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.8e-09
Identity = 34/49 (69.39%), Postives = 42/49 (85.71%), Query Frame = 0


Query: 30  KEEEQAVVAEAPEIRRLGRHHFHTSMAGGGVLIGGLATAVFAVVFCYIR 79
           + +E + VAEAP IRR+G+HH   S+AGGGV+IGGLATA+FAVVFCYIR
Sbjct: 62  ESKEYSDVAEAPVIRRMGKHHSDKSLAGGGVIIGGLATAIFAVVFCYIR 110

[Close]

BLAST of Moc02g05540.1 vs. TAIR 10
Match: AT3G09280.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: root; Has 31 Blast hits to 31 proteins in 9 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 31; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 49.7 bits (117), Expect = 1.4e-06
Identity = 33/73 (45.21%), Postives = 40/73 (54.79%), Query Frame = 0


Query: 14 APAVAAATPYFAAGIGKEEEQAVVAEAPEIRRL------GRHH--FHTSMAGGGVLIGGL 73
          A A + A P     +G+ E     AEAPE+  L      G HH     S+AGGGV++GGL
Sbjct: 19 AAASSEAEPPATRKLGRHEWPGEEAEAPEVSHLEETVRRGHHHSTVERSVAGGGVILGGL 78

Query: 74 ATAVFAVVFCYIR 79
          AT    VVFCYIR
Sbjct: 79 ATTFLVVVFCYIR 91

The following BLAST results are available for this feature:

BLAST of Moc02g05540.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023532519.1	4.5e-18	74.03	uncharacterized protein LOC111794654 [Cucurbita pepo subsp. pepo]	[more]
XP_022947872.1	3.8e-17	74.03	uncharacterized protein LOC111451632 [Cucurbita moschata] >KAG6604835.1 hypothet...	[more]
KAG7034949.1	3.8e-17	74.03	hypothetical protein SDJN02_01742 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KGN46704.1	5.1e-14	66.25	hypothetical protein Csa_020980 [Cucumis sativus]	[more]
KAE8645753.1	3.4e-10	77.08	hypothetical protein Csa_020263 [Cucumis sativus]	[more]

BLAST of Moc02g05540.1 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc02g05540.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G7L7	1.8e-17	74.03	uncharacterized protein LOC111451632 OS=Cucurbita moschata OX=3662 GN=LOC1114516...	[more]
A0A0A0KCY0	2.5e-14	66.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124190 PE=4 SV=1	[more]
A0A200QMD0	2.8e-10	69.81	Uncharacterized protein OS=Macleaya cordata OX=56857 GN=BVC80_8997g5 PE=4 SV=1	[more]
A0A0A0K3N0	1.1e-09	78.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G045480 PE=4 SV=1	[more]
A0A2N9IXN3	1.8e-09	69.39	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS56997 PE=4 SV=1	[more]

BLAST of Moc02g05540.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09280.1	1.4e-06	45.21	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34558:SF4	EXPRESSED PROTEIN	coord: 7..78
None	No IPR available	PANTHER	PTHR34558	EXPRESSED PROTEIN	coord: 7..78

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Moc02g05540	Moc02g05540	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Moc02g05540.1.exon1	Moc02g05540.1.exon1	exon
Moc02g05540.1.exon2	Moc02g05540.1.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Moc02g05540.1	cds.Moc02g05540.1	CDS
cds.Moc02g05540.1	cds.Moc02g05540.1_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Moc02g05540.1	Moc02g05540.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane