MS024456.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS024456.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	scaffold359: 191277 .. 192529 (+)
Sequence length	420
RNA-Seq Expression	MS024456.1
Synteny	MS024456.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGTTCACTTGCTTCCGGCCGCTGCAACAAGGTTTAAATCTCATTTTAGTTTTTGTATGGCCTAAATATTCATAAACCCGAGAATTGATTCGTTCCTAAAAATCAAGTTTTCTAACTCCTCATCGTATAAGTTTTATATTTCTGTCTCGACAGGTAAAGATACTTATATTAAGTTTTTTTTTTTTAACAATTTTTCTTTATCTTGTCCACGATCATCTTCCTTCTAGTTTTTTCTTTTTCTCCTTTTAGGTAGATTTTTGAGTAATATTGGACGAAATTGGTCATTCGAAAAGGAGTGGATCTTCCTCCATATCCTAAAATAATTAATCTCATTATTCGCAAATGTTTGAAAAGATTTAATCCAAACTATATAAAAAAAAAACGACAATGAAACTAAAGTTTAAATTGATGTAGAAACTTGGACAAATATCTCTTGAGGTAGCTTGAGGCAATAACAAGACCTCATACAATATTTTTAGTTTTTTCCAAATCTAAGTACTTGATGTGAAATTTCACTGTCTCCTTTCAAGANNNNNNNNNNNNNNNNNNNNNNATATCTCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAATTTCATTGCCAAGTTTGTCTGGTGTCTAGGGCGCATGTACTAAGGGCGGTGCCACCCCTCTTTATTTCTTTCAACTTATGAGAAAAAAAAAACATTACTTATTATATATTATTTTATGTAAAAATTAAAAAAATATATTATATAAATAAACCCAATTTTTTATTCGTCAAATTATAAATTTAGTTCCTACCATCTAGGTTTGATAGAGTCGTTTAGAGAGTCATGCACTATCTTATATTTAATTTTATCAATGTACATATATAATTACTATTAGACTAGAAGGTAAAGTGGCACTAATCACCGGTGGGGCTAGAGGAATTGGAGAATACACTACAAGACTGTTCTTCAAGCATGGAGCTAAAGTAGTCATTGCTGACATTCAAGACGATTTAGGCCACTCTCTTTCCAACCATCTATGCCCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAAAGAGAATGATGTCGAAAACGCTGTCGATACGACCATCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGGCATTTTTGGAGACCTAAAGTTCAACATTCTCAAAAACGAATCATCAAACTTTCAAAAGGTTCTCAATGTCAACCTCCTCGGAGCCTTTCTTGGGACGAAACACGCGGCGCGAGCGATGATCTAG

mRNA sequence

ATGGGCGTTCACTTGCTTCCGGCCGCTGCAACAAGACTAGAAGGTAAAGTGGCACTAATCACCGGTGGGGCTAGAGGAATTGGAGAATACACTACAAGACTGTTCTTCAAGCATGGAGCTAAAGTAGTCATTGCTGACATTCAAGACGATTTAGGCCACTCTCTTTCCAACCATCTATGCCCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAAAGAGAATGATGTCGAAAACGCTGTCGATACGACCATCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGGCATTTTTGGAGACCTAAAGTTCAACATTCTCAAAAACGAATCATCAAACTTTCAAAAGGTTCTCAATGTCAACCTCCTCGGAGCCTTTCTTGGGACGAAACACGCGGCGCGAGCGATGATCTAG

Coding sequence (CDS)

ATGGGCGTTCACTTGCTTCCGGCCGCTGCAACAAGACTAGAAGGTAAAGTGGCACTAATCACCGGTGGGGCTAGAGGAATTGGAGAATACACTACAAGACTGTTCTTCAAGCATGGAGCTAAAGTAGTCATTGCTGACATTCAAGACGATTTAGGCCACTCTCTTTCCAACCATCTATGCCCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAAAGAGAATGATGTCGAAAACGCTGTCGATACGACCATCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGGCATTTTTGGAGACCTAAAGTTCAACATTCTCAAAAACGAATCATCAAACTTTCAAAAGGTTCTCAATGTCAACCTCCTCGGAGCCTTTCTTGGGACGAAACACGCGGCGCGAGCGATGATCTAG

Protein sequence

MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNLLGAFLGTKHAARAMI

Homology

[Close]

BLAST of MS024456.1 vs. NCBI nr
Match: XP_022142407.1 (momilactone A synthase-like [Momordica charantia])

HSP 1 Score: 254.2 bits (648), Expect = 6.4e-64
Identity = 127/139 (91.37%), Postives = 133/139 (95.68%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MGV LLPAAA RLEGKVALITGGARGIGEYT RLFFKHGAKVVIADIQDDLGHSLSN+LC
Sbjct: 1   MGVPLLPAAARRLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
           PSSSSFVHCDVT+ENDV NAVDTT+SKYGKLDIMFNNAGI GDLKFNIL+NES +FQKVL
Sbjct: 61  PSSSSFVHCDVTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAARAM+
Sbjct: 121 NVNLLGAFLGTKHAARAMM 139

[Close]

BLAST of MS024456.1 vs. NCBI nr
Match: XP_038895162.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 2.6e-57
Identity = 118/139 (84.89%), Postives = 124/139 (89.21%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           M V LLPAAA RLEGKVALITGGARGIGEYT RLFFKHGAKVVIADI+DDLGHSLSNH+ 
Sbjct: 1   MAVPLLPAAARRLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIEDDLGHSLSNHIG 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SS SFVHCDVTKE DVENAVD+ +S YGKLDIMFNNA I G  KFNIL+NESS+FQKVL
Sbjct: 61  SSSFSFVHCDVTKEKDVENAVDSALSDYGKLDIMFNNAAILGAPKFNILENESSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAAR MI
Sbjct: 121 NVNLLGAFLGTKHAARVMI 139

[Close]

BLAST of MS024456.1 vs. NCBI nr
Match: XP_022927566.1 (secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 231.1 bits (588), Expect = 5.8e-57
Identity = 115/139 (82.73%), Postives = 125/139 (89.93%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MG+ LLPAAA RLEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGISLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T ISKYGKLDIM NNAGI G LKFNIL+NE S+FQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAA+AMI
Sbjct: 121 NVNLLGAFLGTKHAAKAMI 139

[Close]

BLAST of MS024456.1 vs. NCBI nr
Match: XP_023520716.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229.9 bits (585), Expect = 1.3e-56
Identity = 116/139 (83.45%), Postives = 123/139 (88.49%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MGV LLPAAA RLEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGVSLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T ISKYGKLDIM NNAGI G LKFNIL NE S+FQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILGNELSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAAR MI
Sbjct: 121 NVNLLGAFLGTKHAARVMI 139

[Close]

BLAST of MS024456.1 vs. NCBI nr
Match: XP_022927564.1 (secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 229.6 bits (584), Expect = 1.7e-56
Identity = 114/139 (82.01%), Postives = 124/139 (89.21%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MG+ LLP AA RLEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGISLLPVAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T ISKYGKLDIM NNAGI G LKFNIL+NE S+FQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAA+AMI
Sbjct: 121 NVNLLGAFLGTKHAAKAMI 139

[Close]

BLAST of MS024456.1 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-39
Identity = 81/135 (60.00%), Postives = 104/135 (77.04%), Query Frame = 0


Query: 5   LLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSS 64
           +L A A RLEGKVALITGGA GIGE T +LF +HGAKV IAD+QD+LGHS+   +  S+S
Sbjct: 7   VLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNS 66

Query: 65  SFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNL 124
           +++HCDVT E+ V+NAVD T+S YGKLDIMF+NAGI    +  I+ NE ++F++V +VN+
Sbjct: 67  TYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNV 126

Query: 125 LGAFLGTKHAARAMI 140
            G FL  KHAAR MI
Sbjct: 127 TGVFLCMKHAARVMI 141

[Close]

BLAST of MS024456.1 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.1e-37
Identity = 79/131 (60.31%), Postives = 98/131 (74.81%), Query Frame = 0


Query: 8   AAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSSFV 67
           A A +L GKVA+ITGGA GIG  T RLF KHGA+VV+ADIQD+LG SL   L P +SS+V
Sbjct: 10  ADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYV 69

Query: 68  HCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNLLGA 127
           HCDVT E DV  AVD  ++++GKLD+MFNNAG+ G   F + +    +F++VL VNL+G 
Sbjct: 70  HCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGP 129

Query: 128 FLGTKHAARAM 139
           FLGTKHAAR M
Sbjct: 130 FLGTKHAARVM 140

[Close]

BLAST of MS024456.1 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.2e-36
Identity = 79/141 (56.03%), Postives = 104/141 (73.76%), Query Frame = 0


Query: 1   MGVHLLPAAAT-RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHL 60
           MG    PA++T RL+ KVA+ITGGA GIGE T +LF ++GAKVVIADI DD G  + N++
Sbjct: 1   MGSTSTPASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNI 60

Query: 61  -CPSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQK 120
             P   SFVHCDVTK+ DV N VDTTI+K+GKLDIMF N G+     ++IL+  + +F++
Sbjct: 61  GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKR 120

Query: 121 VLNVNLLGAFLGTKHAARAMI 140
           V+++N+ GAFL  KHAAR MI
Sbjct: 121 VMDINVYGAFLVAKHAARVMI 141

[Close]

BLAST of MS024456.1 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 7.7e-36
Identity = 78/141 (55.32%), Postives = 103/141 (73.05%), Query Frame = 0


Query: 1   MGVHLLPAAAT-RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHL 60
           MG    P ++T RL+ KVA+ITGGA GIGE T +LF ++GAKVVIADI DD G  + N++
Sbjct: 1   MGSTSTPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNI 60

Query: 61  -CPSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQK 120
             P   SFVHCDVTK+ DV N VDTTI+K+GKLDIMF N G+     ++IL+  + +F++
Sbjct: 61  GSPDVISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKR 120

Query: 121 VLNVNLLGAFLGTKHAARAMI 140
           V+++N+ GAFL  KHAAR MI
Sbjct: 121 VMDINVYGAFLVAKHAARVMI 141

[Close]

BLAST of MS024456.1 vs. ExPASy Swiss-Prot
Match: H9BFQ0 (Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.0e-35
Identity = 77/130 (59.23%), Postives = 100/130 (76.92%), Query Frame = 0


Query: 12  RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSSFVHCDV 71
           RLEGKVA+ITGGA GIG  T  LF ++GAKVVIADIQDDLG +L+  L    + ++HCDV
Sbjct: 10  RLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKL-GGKACYIHCDV 69

Query: 72  TKENDVENAVDTTISKYGKLDIMFNNAGIF--GDLKFNILKNESSNFQKVLNVNLLGAFL 131
           +KE+DV N VDTT++KYG+LDIMFNNAGI     L  +++++E S+  ++L+VNL GAFL
Sbjct: 70  SKEDDVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDLDRLLSVNLGGAFL 129

Query: 132 GTKHAARAMI 140
           G KHA R M+
Sbjct: 130 GAKHATRVMV 138

[Close]

BLAST of MS024456.1 vs. ExPASy TrEMBL
Match: A0A6J1CMN7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 3.1e-64
Identity = 127/139 (91.37%), Postives = 133/139 (95.68%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MGV LLPAAA RLEGKVALITGGARGIGEYT RLFFKHGAKVVIADIQDDLGHSLSN+LC
Sbjct: 1   MGVPLLPAAARRLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
           PSSSSFVHCDVT+ENDV NAVDTT+SKYGKLDIMFNNAGI GDLKFNIL+NES +FQKVL
Sbjct: 61  PSSSSFVHCDVTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAARAM+
Sbjct: 121 NVNLLGAFLGTKHAARAMM 139

[Close]

BLAST of MS024456.1 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.8e-57
Identity = 115/139 (82.73%), Postives = 125/139 (89.93%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MG+ LLPAAA RLEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGISLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T ISKYGKLDIM NNAGI G LKFNIL+NE S+FQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAA+AMI
Sbjct: 121 NVNLLGAFLGTKHAAKAMI 139

[Close]

BLAST of MS024456.1 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 8.2e-57
Identity = 114/139 (82.01%), Postives = 124/139 (89.21%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MG+ LLP AA RLEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGISLLPVAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T ISKYGKLDIM NNAGI G LKFNIL+NE S+FQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAA+AMI
Sbjct: 121 NVNLLGAFLGTKHAAKAMI 139

[Close]

BLAST of MS024456.1 vs. ExPASy TrEMBL
Match: A0A6J1I6S5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471670 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.4e-56
Identity = 114/139 (82.01%), Postives = 123/139 (88.49%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MGV LLPAAA RLEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGVSLLPAAARRLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T I+KYGKLDIM NNAGI G LKFN L+NESS+FQ VL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLG FLGTKHAARAMI
Sbjct: 121 NVNLLGTFLGTKHAARAMI 139

[Close]

BLAST of MS024456.1 vs. ExPASy TrEMBL
Match: A0A6J1EPB9 (secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.8e-56
Identity = 115/139 (82.73%), Postives = 124/139 (89.21%), Query Frame = 0


Query: 1   MGVHLLPAAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLC 60
           MGV LLPAAA  LEGKVA+ITG ARGIGE T +LFFKHGAKVV+ADI DD+GHSLS HLC
Sbjct: 1   MGVSLLPAAARWLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLC 60

Query: 61  PSSSSFVHCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVL 120
            SSSSFVHCDVTKE D+ENAV+T ISKYGKLDIM NNAGI G LKFNIL+NE S+FQKVL
Sbjct: 61  SSSSSFVHCDVTKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVL 120

Query: 121 NVNLLGAFLGTKHAARAMI 140
           NVNLLGAFLGTKHAA+AMI
Sbjct: 121 NVNLLGAFLGTKHAAKAMI 139

[Close]

BLAST of MS024456.1 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-31
Identity = 71/134 (52.99%), Postives = 92/134 (68.66%), Query Frame = 0


Query: 12  RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQD----DLGHSLSNHLCPSSSSFV 71
           RLEGKVA+ITGGA GIG+ T  LF +HGA VVIAD+ +     L  SLS+H      +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 72  HCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLK--FNILKNESSNFQKVLNVNLL 131
            CDV+ E DVEN V+ T+++YG+LDI+FNNAG+ GD K   +IL  ++  F  V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 132 GAFLGTKHAARAMI 140
           G  LG KH ARAMI
Sbjct: 151 GVGLGMKHGARAMI 164

[Close]

BLAST of MS024456.1 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 8.4e-30
Identity = 69/132 (52.27%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 8   AAATRLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSSFV 67
           + + +LEGKVALITGGA GIG+ T   F  HGAKV+IADIQ  +G      L PS + F 
Sbjct: 73  STSRKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPSCAYF- 132

Query: 68  HCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNLLGA 127
            CDVTKE+D+ NAVD  +S + KLDIM+NNAGI      +I+  + + F KV+N N+ G 
Sbjct: 133 PCDVTKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGV 192

Query: 128 FLGTKHAARAMI 140
             G KHAAR MI
Sbjct: 193 MAGIKHAARVMI 203

[Close]

BLAST of MS024456.1 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 1.9e-29
Identity = 61/128 (47.66%), Postives = 87/128 (67.97%), Query Frame = 0


Query: 12  RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSSFVHCDV 71
           RL+GK+ +ITGGA GIG  + RLF +HGA+VVI D+QD+LG +++  +    +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 72  TKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNLLGAFLGT 131
           T E +VENAV  T+ KYGKLD++F+NAG+      +IL    +   + + +NL G     
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAAFI 124

Query: 132 KHAARAMI 140
           KHAARAM+
Sbjct: 125 KHAARAMV 131

[Close]

BLAST of MS024456.1 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 125.2 bits (313), Expect = 4.2e-29
Identity = 69/132 (52.27%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 12  RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSS----FV 71
           RL GKVALITGGA GIGE   RLF KHGAKV I D+QDDLG  +   L    S     F+
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 72  HCDVTKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNLLGA 131
           H DV  E+D+ NAVD  +  +G LDI+ NNAG+ G    +I     S F+   +VN+ GA
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 132 FLGTKHAARAMI 140
           FL  KHAAR MI
Sbjct: 137 FLSMKHAARVMI 148

[Close]

BLAST of MS024456.1 vs. TAIR 10
Match: AT2G47120.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 7.1e-29
Identity = 62/128 (48.44%), Postives = 88/128 (68.75%), Query Frame = 0


Query: 12  RLEGKVALITGGARGIGEYTTRLFFKHGAKVVIADIQDDLGHSLSNHLCPSSSSFVHCDV 71
           RLEGK+ +ITGGA GIG    RLF  HGAKVVI D+Q++LG +++  +    +SF  CDV
Sbjct: 5   RLEGKIVIITGGASGIGADAARLFTDHGAKVVIVDVQEELGQNVAVLIGKDKASFYRCDV 64

Query: 72  TKENDVENAVDTTISKYGKLDIMFNNAGIFGDLKFNILKNESSNFQKVLNVNLLGAFLGT 131
           T E +VE+AV  T+ K+GKLD++F+NAG+   L+ + L  +   F +++ VN+ GA    
Sbjct: 65  TNETEVEDAVKFTVEKHGKLDVLFSNAGVLEPLE-SFLDFDLERFDRIMAVNVRGAAAFI 124

Query: 132 KHAARAMI 140
           KHAARAM+
Sbjct: 125 KHAARAMV 131

The following BLAST results are available for this feature:

BLAST of MS024456.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142407.1	6.4e-64	91.37	momilactone A synthase-like [Momordica charantia]	[more]
XP_038895162.1	2.6e-57	84.89	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]
XP_022927566.1	5.8e-57	82.73	secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata]	[more]
XP_023520716.1	1.3e-56	83.45	secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022927564.1	1.7e-56	82.01	secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata]	[more]

BLAST of MS024456.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	1.5e-39	60.00	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	1.1e-37	60.31	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
A3F5F0	1.2e-36	56.03	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q94KL8	7.7e-36	55.32	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
H9BFQ0	1.0e-35	59.23	Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]

BLAST of MS024456.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMN7	3.1e-64	91.37	momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 ...	[more]
A0A6J1EI14	2.8e-57	82.73	secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EID0	8.2e-57	82.01	secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1I6S5	1.4e-56	82.01	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A6J1EPB9	1.8e-56	82.73	secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]

BLAST of MS024456.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	1.2e-31	52.99	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	8.4e-30	52.27	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	1.9e-29	47.66	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	4.2e-29	52.27	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47120.1	7.1e-29	48.44	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 17..34 score: 47.07 coord: 89..100 score: 58.12
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 16..139 e-value: 3.7E-28 score: 98.3
None	No IPR available	GENE3D	3.40.50.720		coord: 7..139 e-value: 1.8E-37 score: 130.8
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 12..123 e-value: 2.5E-5 score: 21.4
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 8..139
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 8..139
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 14..139

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS024456	MS024456	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS024456.1-cds	MS024456.1-cds-scaffold359:191277..191311	CDS
MS024456.1-cds	MS024456.1-cds-scaffold359:192145..192529	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS024456.1	MS024456.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity