MS024162.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS024162.1 vs. NCBI nr
Match: XP_022131895.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 534/589 (90.66%), Postives = 563/589 (95.59%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFII NE FEKI+SLGL+P M+FY R
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D+YGF+IAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVI+LGSFCSFLGMALLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
            IPQLKPSSCSSSGILCDSANLYQLA+LFTS+GFISIGAGCIKPCSMAFGADQLTNEK  
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           +N+GVLDTYFNWYYA+SG+STVIGMT+IVYIQDEFGW TGY VPAVLML SALIFLAGSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KPAQSLFTGI QVIVVAFKNRRL LPYSNFDQYYLGHDPK LVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DAENDLNPDGSVSNPWNLCSVDQVESLKNF+RIIP+WSTGIIM VC++QN+ G LQA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           +IMNRHITG+FE PAGSI VFMIISLSLWLVFYDRII+PLLKRCGHAGRL PELRIGIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+AVVE TRRRIAIEEGLEDQPNA++DMSALW+VPQCVVLGVAEGFNAVGQI
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYFF +I K SSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNE SWLDNNLNKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALALVNLCGYLLYNRAYGFSEK QGNESSEIQ+FEYR LPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MS024162.1 vs. NCBI nr
Match: XP_022132097.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.8e-294
Identity = 505/589 (85.74%), Postives = 550/589 (93.38%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEVVEN EKTGIA+SS EGTHQASNCRTGGLRTMPFIIVNE+FEKIASLGLTP MVFYLR
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           DDYGF+IAAASS+LSLWSAASNAL+IAGAVLADSY+GRFR I+LGSF SFLGMALLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           MIPQLKPSSCSSSGILCDSAN YQLAILFTS+GFISIGAGCI+PCS+AFGADQLTNEK  
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NNEGVLDTYFNWYYASSG+++VI +TLIVYIQDEFGWRTG+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG+LQVIVVAFKNRRL LPYSN+DQYYLG DPKFLVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DAENDLNPDGS+SNPWNLCSV+ VESLKNFLRIIPMWSTGI+M VC++QNSFGTLQA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHITG+FEIPAGSI V MIISLS+WL FYDRI++PLL+RCGHAG L P LR+GIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAISAVVEGTRRRIA EEGLEDQP A+IDMS LWIVPQCVVLGVAEGFNA+GQI
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNE SWLDNNLNKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYLL NR YG SE R+ NES+E+Q+FEYRKLPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MS024162.1 vs. NCBI nr
Match: XP_022962308.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 880.9 bits (2275), Expect = 5.9e-252
Identity = 436/589 (74.02%), Postives = 512/589 (86.93%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEV+E+ EKTGIA S  E   QASN   GGL+TMPFIIVNE+FE++ASLGLTP MVFYLR
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D +GF IAAAS +LSLWSAASNAL+I GAVLADSY+GRF VI+LGS  S LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           +IPQL+P+SCS SGILCDSAN YQLA+LF+S+ FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NNE VLD+YFNWYYAS G++T+I ++LIV+IQD+FGW  G+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG L+VIVVAFKNR+L LP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DA+N+LNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGI+M VC++QNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHIT +FEIPAGSIT+FM++SL  W++FYDRI++PLL R GHAG L P+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAISA V   RRR+AIE+G EDQPNA+I+MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+ SWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYL+Y  A G    RQ  E +E+++FEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVYCWALG----RQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024162.1 vs. NCBI nr
Match: KAG6598782.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 877.1 bits (2265), Expect = 8.6e-251
Identity = 434/589 (73.68%), Postives = 510/589 (86.59%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEV+E+ EK+GIA S  EG  QASN   GGL+TMPFIIVNE+FE++ASLGLTP MVFYLR
Sbjct: 1   MEVLEDGEKSGIAASGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D +GF IAAAS +LSLWSAASNAL+I GAVLADSY+GRF VI+LGS  S LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           +IPQL+P SCS SGILCDSAN YQLA+LF+S+ FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPESCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NNE VLD+YFNWYYAS G++T++ ++LIV+IQD+FGW  G+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIVALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG L+VIVVAFKNR+L LP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DA+N+LNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGI+M VC++QNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHIT +FEIPAGSIT+FM++SL  W++FYDRI++PLL R GHAG L P+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAISA V   RRR+AIE+G EDQPNA+I+MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL++ EM VANLVGSI+VQ+VN ITG+GN+ SWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMVVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYL+   A G    RQ  E +E+++FEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALG----RQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024162.1 vs. NCBI nr
Match: XP_023545603.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 876.7 bits (2264), Expect = 1.1e-250
Identity = 434/589 (73.68%), Postives = 510/589 (86.59%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEV+E+ EKTGIA S  EG  QASN   GGL+TMPFIIVNE+FE++ASLGLTP MVFYLR
Sbjct: 1   MEVLEDGEKTGIAVSGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D +GF+IA AS +LSLWSAASNAL+I GAVLADSY+GRF VI+LGS  S LGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           +IPQL+P+SCS SGILCDSAN YQLA+LF+S+ FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NN+ VLD+YFNWYYAS G++T+I ++LIV+IQD+FGW  G+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNDSVLDSYFNWYYASIGIATIIALSLIVFIQDQFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG L+VIVVAFKNR+L LP+SNFDQYYLG DPK  +PTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCPIPTDSMRCLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+D +N+LNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGI+M VC++QNSFGT+QA
Sbjct: 301 LIKDGKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHIT  FEIPAGSIT+FM++SL  W++FYDRI++PLL R GHAG L P+LRIGIGL
Sbjct: 361 KTMNRHITRTFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLS FAMAISA V   RRR+AIE+G EDQPNA+I+MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSFFAMAISAGVAYIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+ SWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYL+   A G    RQ  E +E+++FEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALG----RQHKEPNEMEDFEYRNLPSS 585

BLAST of MS024162.1 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 9.5e-152
Identity = 265/545 (48.62%), Postives = 384/545 (70.46%), Query Frame = 0

Query: 29  GGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSIAG 88
           GG+ TMPFII NE+FEK+AS GL P M+ YL  DY F +A  ++VL +WSAASN   + G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSC--SSSGILCDSANLYQLA 148
           A L+DSY+GRF  I + S  SFLGM LLWLTAM+PQ+KPS C  +++G  C S+   QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 ILFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMT 208
           +L+++   ISIG+G I+PCS+AFGADQL N++   NE VL+++F WYYASS V+ +I  T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 LIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNR 268
            IVYIQ+  GW+ G+ VPAVLML +AL+F+  S LYV     +SLFTG+ Q IV A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLPLP--YSNFDQYYLGHDPKFLVPTDSMRCLNKACLIRDAENDLNPDGSVSNPWNLCSV 328
           +L LP  + +FD YY   D +   P+  +R LNKACLI + E ++  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 DQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQAEIMNRHIT---GNFEIPAGSITVF 388
           D+VE LK  +++IP+WSTGI+M++   Q+SF  LQA  M+R ++    +F++PAGS  +F
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MIISLSLWLVFYDRIIIPLLKRC-GHAGRLRPELRIGIGLLLSCFAMAISAVVEGTRRRI 448
            II+L+LW++ YDR +IPL  +  G   RL  +LR+G+GL +S  AMAISA+VE  RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 AIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAVALHT 508
           AI +G  +  NA++D+SA+W+VPQ V+ G+AE   A+GQ E+F+ + PK+ SS+A +L  
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 566
           + M+VA+L+ S+++  VN +T +  + SW+ +N+NKGH +Y+YW+ A ++ +N+  Y++ 
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of MS024162.1 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 523.1 bits (1346), Expect = 4.1e-147
Identity = 260/543 (47.88%), Postives = 373/543 (68.69%), Query Frame = 0

Query: 29  GGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSIAG 88
           GGL TMPFII NE FEK+AS GL   M+ YL  DY   +    +VL +W AA+N + + G
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSC-SSSGILCDSANLYQLAI 148
           A L+DSY+GRF  IV+ S  S LGM +LWLTAM+PQ+KPS C +++G  C SA   QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMTL 208
           L+T+   ISIG+G I+PCS+AFGADQL N++   NE VL+++F WYYASS V+ +I  T+
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNRR 268
           IVYIQD  GW+ G+ +PA+LML +  +F+  S LYVK   ++SLFTG+ QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LPLP--YSNFDQYYLGHDPKFLVPTDSMRCLNKACLIRDAENDLNPDGSVSNPWNLCSVD 328
           L LP  + + D YY   D +   P+D +R LNKAC I + + DL  DG   N W LC+ D
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 QVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQAEIMNRHITGN--FEIPAGSITVFMI 388
           QVE LK  +++IP+WSTGI+M++ V QNSF  LQA+ M+R ++ N  F+IPAGS  +F I
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ISLSLWLVFYDRIIIPLLKRC-GHAGRLRPELRIGIGLLLSCFAMAISAVVEGTRRRIAI 448
           I+L  W+V YDR I+PL  +  G   R+  ++R+G+GL +S  AMA+SA VE  RR+ AI
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAVALHTVE 508
            +GL +  N+ + +SA+W+VPQ V+ G+AE    +GQ E+F+ + PK+ SS+A +L  + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLYNR 566
           M+VAN++ S+++  V   + +GN  SW+++N+NKGH DY+YW+ A L+ VN+  Y++ + 
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

BLAST of MS024162.1 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 7.1e-107
Identity = 208/550 (37.82%), Postives = 327/550 (59.45%), Query Frame = 0

Query: 27  RTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSI 86
           + GG R + FI+ NE+ E++ S+GL    + YL   +      A++V+++WS  +N   +
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 87  AGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSCSSSGIL-CDSANLYQL 146
            GA ++D+Y+GRF+ I   SF + LG+  + LTA  PQL P+SC+S   L C   N  Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 147 AILFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGM 206
            +L   + F+S+G+G I+PCS+ FG DQ         +GV  ++FNWYY +  V  +I  
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQ 230

Query: 207 TLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKN 266
           T++VYIQD+  W  G+++P  LM  + ++F AG   YV +KP  S+F+GI QVIV A K 
Sbjct: 231 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 290

Query: 267 RRLPLPYSNFDQYYLGHDPKF-------LVPTDSMRCLNKACLIRDAENDLNPDGSVSNP 326
           R+L LP  + D     +DP         L  ++  RCL+KA ++   E DL P+G  ++ 
Sbjct: 291 RKLKLPAED-DGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADK 350

Query: 327 WNLCSVDQVESLKNFLRIIPMWSTGII-MTVCVHQNSFGTLQAEIMNRHITGNFEIPAGS 386
           W LCSV +VE +K  +RI+P+WS GII +     Q +F   QA  M+R++   FEIPAGS
Sbjct: 351 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 410

Query: 387 ITVFMIISLSLWLVFYDRIIIPLLKR-CGHAGRLRPELRIGIGLLLSCFAMAISAVVEGT 446
           ++V  ++++ ++L FYDR+ +P ++R  GH   +    RIG G++ + F+M ++ +VE  
Sbjct: 411 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 470

Query: 447 RRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAV 506
           RR  +I  G    P  +  MS  W+ PQ +++G+ E FN +GQIE+F  Q P+   S+A 
Sbjct: 471 RRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIAN 530

Query: 507 ALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCG 566
           +L ++  + ++ + S LV +V+  +G  + P WL+ NLN G LDYFY+L A L +VNL  
Sbjct: 531 SLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVY 590

BLAST of MS024162.1 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 2.7e-98
Identity = 209/558 (37.46%), Postives = 325/558 (58.24%), Query Frame = 0

Query: 21  HQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAA 80
           H   N   GGL TMPFI  NE  EK+A +G    M+ YL       +  A++ L+ ++  
Sbjct: 17  HGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGT 76

Query: 81  SNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSCSSSGILCDSA 140
           S+   + GA +ADS+ GRF  I   S    +GM LL ++A+IP L+P  C    + C  A
Sbjct: 77  SSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV-CVVA 136

Query: 141 NLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVS 200
           +  QL+IL+ ++   ++G+G I+PC +AFGADQ  +E   N       YFNWYY   G +
Sbjct: 137 DTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQTTKTWNYFNWYYFCMGAA 196

Query: 201 TVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIV 260
            ++ +T++V+IQD  GW  G  +P V M  S + F+ G  LY  + PA S FT ++QV V
Sbjct: 197 VLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGV 256

Query: 261 VAFKNRRLPLPYSNFDQYY---------LGHDPKFLVPTDSMRCLNKACLIRDAENDLNP 320
            AF+ R+L +       Y+         LG     L  T  M  L+KA ++ + E++L P
Sbjct: 257 AAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMSFLDKAAIVTE-EDNLKP 316

Query: 321 DGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIM-TVCVHQNSFGTLQAEIMNRHITGN 380
            G + N W L +V +VE LK+ +R+ P+ ++GI++ T    Q +F   QA+ MNRH+T +
Sbjct: 317 -GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNS 376

Query: 381 FEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPEL-RIGIGLLLSCFAMAI 440
           F+IPAGS++VF  +++   ++FYDR+ + + ++     R    L R+GIG ++S  A  +
Sbjct: 377 FQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLV 436

Query: 441 SAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPK 500
           +  VE  R+ +AIE GL D+P+ I+ +S LW++PQ  + GVAE F ++G +E+F+ Q P+
Sbjct: 437 AGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPE 496

Query: 501 ASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWL-DNNLNKGHLDYFYWLCAA 560
           +  S A AL  + +S+ N V ++LV +V+  + K +  +WL DNNLN+G L+YFYWL   
Sbjct: 497 SMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITV 556

Query: 561 LALVNLCGYLLYNRAYGF 567
           L  VNL  YL   + Y +
Sbjct: 557 LQAVNLVYYLWCAKIYTY 567

BLAST of MS024162.1 vs. ExPASy Swiss-Prot
Match: Q9M9V7 (Protein NRT1/ PTR FAMILY 2.9 OS=Arabidopsis thaliana OX=3702 GN=NPF2.9 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.7e-97
Identity = 199/566 (35.16%), Postives = 332/566 (58.66%), Query Frame = 0

Query: 30  GLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSIAGA 89
           G + MPFII NE+FEK+  +G +  +V YL   +  +   A+ V++++   SN  +I  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 90  VLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSCSSS-GILCDSANLYQLAIL 149
            L DSY GR++ +       FLG   + LTA+I  L P+ C+   G +C+  ++ Q+  L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 150 FTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMTLI 209
             +M  + IGAG I+PC++ FGADQ  + K    +  ++++FNWY+ +   + ++ +TLI
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQF-DPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLI 201

Query: 210 VYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNRRL 269
           VY+Q    W  G A+PA+LML   +IF AGS LYVK+K + S    I +VIVVA K RRL
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 270 PLPYSNFDQYYLGHDPK--FLVPTDSMRCLNKACLIRDAENDLNPDGSVSNPWNLCSVDQ 329
                N    Y+  D K   L  T+  R L+K+  I+  ++ LN DGS  + W LCS+ Q
Sbjct: 262 KPVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 330 VESLKNFLRIIPMW-STGIIMTVCVHQNSFGTLQAEIMNRHI-TGNFEIPAGSITVFMII 389
           VE +K  +R++P+W S  +     + Q ++   Q+   +R +  G+F+IPAGS TVF+++
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 390 SLSLWLVFYDRIIIPLLKR-CGHAGRLRPELRIGIGLLLSCFAMAISAVVEGTRRRIAIE 449
            +++++  YDR+++P L++  G  G +    R+G GL L   +M +SA+VE  RR++A+ 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 450 E---GLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAVALHT 509
           +   GL  +  AI  MS +W++PQ V++G+A+    VGQ+E+++ Q P+   S A +L+ 
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 510 VEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 569
             + +A+ + + L+  V+  T   +  SWL  +LNKG L+YFY+L A +  +NL  +LL 
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLLV 561

Query: 570 NRAYGFSEKRQGNESSEIQNFEYRKL 587
           +  Y + +    ++  +  + E+ K+
Sbjct: 562 SHWYRYKDVVAKDKDMDKTSAEFDKV 585

BLAST of MS024162.1 vs. ExPASy TrEMBL
Match: A0A6J1BQY7 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925 PE=3 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 534/589 (90.66%), Postives = 563/589 (95.59%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFII NE FEKI+SLGL+P M+FY R
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D+YGF+IAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVI+LGSFCSFLGMALLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
            IPQLKPSSCSSSGILCDSANLYQLA+LFTS+GFISIGAGCIKPCSMAFGADQLTNEK  
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           +N+GVLDTYFNWYYA+SG+STVIGMT+IVYIQDEFGW TGY VPAVLML SALIFLAGSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KPAQSLFTGI QVIVVAFKNRRL LPYSNFDQYYLGHDPK LVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DAENDLNPDGSVSNPWNLCSVDQVESLKNF+RIIP+WSTGIIM VC++QN+ G LQA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           +IMNRHITG+FE PAGSI VFMIISLSLWLVFYDRII+PLLKRCGHAGRL PELRIGIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAI+AVVE TRRRIAIEEGLEDQPNA++DMSALW+VPQCVVLGVAEGFNAVGQI
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYFF +I K SSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNE SWLDNNLNKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALALVNLCGYLLYNRAYGFSEK QGNESSEIQ+FEYR LPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MS024162.1 vs. ExPASy TrEMBL
Match: A0A6J1BSW7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 2.3e-294
Identity = 505/589 (85.74%), Postives = 550/589 (93.38%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEVVEN EKTGIA+SS EGTHQASNCRTGGLRTMPFIIVNE+FEKIASLGLTP MVFYLR
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           DDYGF+IAAASS+LSLWSAASNAL+IAGAVLADSY+GRFR I+LGSF SFLGMALLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           MIPQLKPSSCSSSGILCDSAN YQLAILFTS+GFISIGAGCI+PCS+AFGADQLTNEK  
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NNEGVLDTYFNWYYASSG+++VI +TLIVYIQDEFGWRTG+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG+LQVIVVAFKNRRL LPYSN+DQYYLG DPKFLVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DAENDLNPDGS+SNPWNLCSV+ VESLKNFLRIIPMWSTGI+M VC++QNSFGTLQA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHITG+FEIPAGSI V MIISLS+WL FYDRI++PLL+RCGHAG L P LR+GIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAISAVVEGTRRRIA EEGLEDQP A+IDMS LWIVPQCVVLGVAEGFNA+GQI
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNE SWLDNNLNKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYLL NR YG SE R+ NES+E+Q+FEYRKLPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MS024162.1 vs. ExPASy TrEMBL
Match: A0A6J1HEG1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462798 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.9e-252
Identity = 436/589 (74.02%), Postives = 512/589 (86.93%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEV+E+ EKTGIA S  E   QASN   GGL+TMPFIIVNE+FE++ASLGLTP MVFYLR
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D +GF IAAAS +LSLWSAASNAL+I GAVLADSY+GRF VI+LGS  S LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           +IPQL+P+SCS SGILCDSAN YQLA+LF+S+ FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NNE VLD+YFNWYYAS G++T+I ++LIV+IQD+FGW  G+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG L+VIVVAFKNR+L LP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DA+N+LNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGI+M VC++QNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHIT +FEIPAGSIT+FM++SL  W++FYDRI++PLL R GHAG L P+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAISA V   RRR+AIE+G EDQPNA+I+MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+ SWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYL+Y  A G    RQ  E +E+++FEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVYCWALG----RQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024162.1 vs. ExPASy TrEMBL
Match: A0A6J1K947 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491783 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 9.2e-251
Identity = 434/589 (73.68%), Postives = 512/589 (86.93%), Query Frame = 0

Query: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLR 60
           MEV+E+ EKTGI+ S  EGT QASN   GGL+TMPFIIVNE+FE++ASLGLTP MVFYLR
Sbjct: 1   MEVLEDGEKTGISASGSEGTEQASNHTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFRIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTA 120
           D +GF+IA AS +LSLWSAASNAL+I GAVLADSY+GRF VI+LGS  S LGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKIL 180
           +IPQL+P+SCS SGILCDSAN YQLA+LF+S+ FISIGAGCI+PCS+AFGADQLT E+  
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSS 240
           NNE VLD+YFNWYYAS G++T+  ++LIV+IQD+FGW  G+AVPAVLMLFS LIFL GSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTDSMRCLNKAC 300
           LYVK+KP QSL TG L+VIVVAFKNR+L LP+SNFDQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNSMRYLNKAC 300

Query: 301 LIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQA 360
           LI+DA+N+LNPDGSVSNPWNLCSVDQVESLK FLRIIPMWSTGI+M VC++QNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 EIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPELRIGIGL 420
           + MNRHIT +FEIPAGSIT+FM++SL  W++FYDRI++PLL R GHAG L P+LRIGIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQI 480
           LLSCFAMAISA V   RRR+AIE+G EDQPNA+I+MSALW+VPQCVVLGVAE  N+VGQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLD 540
           EYF+ Q+PK  SS+AVAL++ EM+VANLVGSI+VQ+VN ITG+GN+ SWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           YFYWLCAALAL+N  GYL+   A G    RQ  E +E+++FEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALG----RQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024162.1 vs. ExPASy TrEMBL
Match: A0A6J1BVB4 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.2e-237
Identity = 406/478 (84.94%), Postives = 444/478 (92.89%), Query Frame = 0

Query: 112 GMALLWLTAMIPQLKPSSCSSSGILCDSANLYQLAILFTSMGFISIGAGCIKPCSMAFGA 171
           GMALLWLTAMIPQLKPSSCSSSGILCDSAN YQLAILFTS+GFISIGAGCI+PCS+AFGA
Sbjct: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79

Query: 172 DQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMTLIVYIQDEFGWRTGYAVPAVLMLFS 231
           DQLTNEK  NNEGVLDTYFNWYYASSG+++VI +TLIVYIQDEFGWRTG+AVPAVLMLFS
Sbjct: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139

Query: 232 ALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNRRLPLPYSNFDQYYLGHDPKFLVPTD 291
            LIFL GSSLYVK+KP QSL TG+LQVIVVAFKNRRL LPYSN+DQYYLG DPKFLVPTD
Sbjct: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199

Query: 292 SMRCLNKACLIRDAENDLNPDGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIMTVCVH 351
           SMRCLNKACLI+DAENDLNPDGS+SNPWNLCSV+ VESLKNFLRIIPMWSTGI+M VC++
Sbjct: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259

Query: 352 QNSFGTLQAEIMNRHITGNFEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLR 411
           QNSFGTLQA+ MNRHITG+FEIPAGSI V MIISLS+WL FYDRI++PLL+RCGHAG L 
Sbjct: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319

Query: 412 PELRIGIGLLLSCFAMAISAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVA 471
           P LR+GIGLLLSCFAMAISAVVEGTRRRIA EEGLEDQP A+IDMS LWIVPQCVVLGVA
Sbjct: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 379

Query: 472 EGFNAVGQIEYFFLQIPKASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLD 531
           EGFNA+GQIEYF+ Q+PK  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNE SWLD
Sbjct: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439

Query: 532 NNLNKGHLDYFYWLCAALALVNLCGYLLYNRAYGFSEKRQGNESSEIQNFEYRKLPSS 590
           NNLNKGHLDYFYWLCAALAL+N  GYLL NR YG SE R+ NES+E+Q+FEYRKLPSS
Sbjct: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 497

BLAST of MS024162.1 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 538.5 bits (1386), Expect = 6.7e-153
Identity = 265/545 (48.62%), Postives = 384/545 (70.46%), Query Frame = 0

Query: 29  GGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSIAG 88
           GG+ TMPFII NE+FEK+AS GL P M+ YL  DY F +A  ++VL +WSAASN   + G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSC--SSSGILCDSANLYQLA 148
           A L+DSY+GRF  I + S  SFLGM LLWLTAM+PQ+KPS C  +++G  C S+   QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 ILFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMT 208
           +L+++   ISIG+G I+PCS+AFGADQL N++   NE VL+++F WYYASS V+ +I  T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 LIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNR 268
            IVYIQ+  GW+ G+ VPAVLML +AL+F+  S LYV     +SLFTG+ Q IV A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLPLP--YSNFDQYYLGHDPKFLVPTDSMRCLNKACLIRDAENDLNPDGSVSNPWNLCSV 328
           +L LP  + +FD YY   D +   P+  +R LNKACLI + E ++  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 DQVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQAEIMNRHIT---GNFEIPAGSITVF 388
           D+VE LK  +++IP+WSTGI+M++   Q+SF  LQA  M+R ++    +F++PAGS  +F
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MIISLSLWLVFYDRIIIPLLKRC-GHAGRLRPELRIGIGLLLSCFAMAISAVVEGTRRRI 448
            II+L+LW++ YDR +IPL  +  G   RL  +LR+G+GL +S  AMAISA+VE  RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 AIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAVALHT 508
           AI +G  +  NA++D+SA+W+VPQ V+ G+AE   A+GQ E+F+ + PK+ SS+A +L  
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 566
           + M+VA+L+ S+++  VN +T +  + SW+ +N+NKGH +Y+YW+ A ++ +N+  Y++ 
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of MS024162.1 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 523.1 bits (1346), Expect = 2.9e-148
Identity = 260/543 (47.88%), Postives = 373/543 (68.69%), Query Frame = 0

Query: 29  GGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSIAG 88
           GGL TMPFII NE FEK+AS GL   M+ YL  DY   +    +VL +W AA+N + + G
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSC-SSSGILCDSANLYQLAI 148
           A L+DSY+GRF  IV+ S  S LGM +LWLTAM+PQ+KPS C +++G  C SA   QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMTL 208
           L+T+   ISIG+G I+PCS+AFGADQL N++   NE VL+++F WYYASS V+ +I  T+
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNRR 268
           IVYIQD  GW+ G+ +PA+LML +  +F+  S LYVK   ++SLFTG+ QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LPLP--YSNFDQYYLGHDPKFLVPTDSMRCLNKACLIRDAENDLNPDGSVSNPWNLCSVD 328
           L LP  + + D YY   D +   P+D +R LNKAC I + + DL  DG   N W LC+ D
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 QVESLKNFLRIIPMWSTGIIMTVCVHQNSFGTLQAEIMNRHITGN--FEIPAGSITVFMI 388
           QVE LK  +++IP+WSTGI+M++ V QNSF  LQA+ M+R ++ N  F+IPAGS  +F I
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ISLSLWLVFYDRIIIPLLKRC-GHAGRLRPELRIGIGLLLSCFAMAISAVVEGTRRRIAI 448
           I+L  W+V YDR I+PL  +  G   R+  ++R+G+GL +S  AMA+SA VE  RR+ AI
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAVALHTVE 508
            +GL +  N+ + +SA+W+VPQ V+ G+AE    +GQ E+F+ + PK+ SS+A +L  + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLYNR 566
           M+VAN++ S+++  V   + +GN  SW+++N+NKGH DY+YW+ A L+ VN+  Y++ + 
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

BLAST of MS024162.1 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 389.4 bits (999), Expect = 5.0e-108
Identity = 208/550 (37.82%), Postives = 327/550 (59.45%), Query Frame = 0

Query: 27  RTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSI 86
           + GG R + FI+ NE+ E++ S+GL    + YL   +      A++V+++WS  +N   +
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 87  AGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSCSSSGIL-CDSANLYQL 146
            GA ++D+Y+GRF+ I   SF + LG+  + LTA  PQL P+SC+S   L C   N  Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 147 AILFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGM 206
            +L   + F+S+G+G I+PCS+ FG DQ         +GV  ++FNWYY +  V  +I  
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQ 230

Query: 207 TLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKN 266
           T++VYIQD+  W  G+++P  LM  + ++F AG   YV +KP  S+F+GI QVIV A K 
Sbjct: 231 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 290

Query: 267 RRLPLPYSNFDQYYLGHDPKF-------LVPTDSMRCLNKACLIRDAENDLNPDGSVSNP 326
           R+L LP  + D     +DP         L  ++  RCL+KA ++   E DL P+G  ++ 
Sbjct: 291 RKLKLPAED-DGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADK 350

Query: 327 WNLCSVDQVESLKNFLRIIPMWSTGII-MTVCVHQNSFGTLQAEIMNRHITGNFEIPAGS 386
           W LCSV +VE +K  +RI+P+WS GII +     Q +F   QA  M+R++   FEIPAGS
Sbjct: 351 WRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGS 410

Query: 387 ITVFMIISLSLWLVFYDRIIIPLLKR-CGHAGRLRPELRIGIGLLLSCFAMAISAVVEGT 446
           ++V  ++++ ++L FYDR+ +P ++R  GH   +    RIG G++ + F+M ++ +VE  
Sbjct: 411 LSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERM 470

Query: 447 RRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAV 506
           RR  +I  G    P  +  MS  W+ PQ +++G+ E FN +GQIE+F  Q P+   S+A 
Sbjct: 471 RRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIAN 530

Query: 507 ALHTVEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCG 566
           +L ++  + ++ + S LV +V+  +G  + P WL+ NLN G LDYFY+L A L +VNL  
Sbjct: 531 SLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVY 590

BLAST of MS024162.1 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.9e-99
Identity = 209/558 (37.46%), Postives = 325/558 (58.24%), Query Frame = 0

Query: 21  HQASNCRTGGLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAA 80
           H   N   GGL TMPFI  NE  EK+A +G    M+ YL       +  A++ L+ ++  
Sbjct: 17  HGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGT 76

Query: 81  SNALSIAGAVLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSCSSSGILCDSA 140
           S+   + GA +ADS+ GRF  I   S    +GM LL ++A+IP L+P  C    + C  A
Sbjct: 77  SSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV-CVVA 136

Query: 141 NLYQLAILFTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVS 200
           +  QL+IL+ ++   ++G+G I+PC +AFGADQ  +E   N       YFNWYY   G +
Sbjct: 137 DTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQTTKTWNYFNWYYFCMGAA 196

Query: 201 TVIGMTLIVYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIV 260
            ++ +T++V+IQD  GW  G  +P V M  S + F+ G  LY  + PA S FT ++QV V
Sbjct: 197 VLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGV 256

Query: 261 VAFKNRRLPLPYSNFDQYY---------LGHDPKFLVPTDSMRCLNKACLIRDAENDLNP 320
            AF+ R+L +       Y+         LG     L  T  M  L+KA ++ + E++L P
Sbjct: 257 AAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMSFLDKAAIVTE-EDNLKP 316

Query: 321 DGSVSNPWNLCSVDQVESLKNFLRIIPMWSTGIIM-TVCVHQNSFGTLQAEIMNRHITGN 380
            G + N W L +V +VE LK+ +R+ P+ ++GI++ T    Q +F   QA+ MNRH+T +
Sbjct: 317 -GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNS 376

Query: 381 FEIPAGSITVFMIISLSLWLVFYDRIIIPLLKRCGHAGRLRPEL-RIGIGLLLSCFAMAI 440
           F+IPAGS++VF  +++   ++FYDR+ + + ++     R    L R+GIG ++S  A  +
Sbjct: 377 FQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLV 436

Query: 441 SAVVEGTRRRIAIEEGLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPK 500
           +  VE  R+ +AIE GL D+P+ I+ +S LW++PQ  + GVAE F ++G +E+F+ Q P+
Sbjct: 437 AGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPE 496

Query: 501 ASSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNEPSWL-DNNLNKGHLDYFYWLCAA 560
           +  S A AL  + +S+ N V ++LV +V+  + K +  +WL DNNLN+G L+YFYWL   
Sbjct: 497 SMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITV 556

Query: 561 LALVNLCGYLLYNRAYGF 567
           L  VNL  YL   + Y +
Sbjct: 557 LQAVNLVYYLWCAKIYTY 567

BLAST of MS024162.1 vs. TAIR 10
Match: AT1G18880.1 (Major facilitator superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 4.7e-98
Identity = 199/566 (35.16%), Postives = 332/566 (58.66%), Query Frame = 0

Query: 30  GLRTMPFIIVNESFEKIASLGLTPTMVFYLRDDYGFRIAAASSVLSLWSAASNALSIAGA 89
           G + MPFII NE+FEK+  +G +  +V YL   +  +   A+ V++++   SN  +I  A
Sbjct: 22  GWKVMPFIIGNETFEKLGIVGSSSNLVIYLTTVFNMKSITAAKVVNIYGGTSNFGTIVAA 81

Query: 90  VLADSYMGRFRVIVLGSFCSFLGMALLWLTAMIPQLKPSSCSSS-GILCDSANLYQLAIL 149
            L DSY GR++ +       FLG   + LTA+I  L P+ C+   G +C+  ++ Q+  L
Sbjct: 82  FLCDSYFGRYKTLSFAMIACFLGSVAMDLTAVIHPLHPAQCAKEIGSVCNGPSIGQIMFL 141

Query: 150 FTSMGFISIGAGCIKPCSMAFGADQLTNEKILNNEGVLDTYFNWYYASSGVSTVIGMTLI 209
             +M  + IGAG I+PC++ FGADQ  + K    +  ++++FNWY+ +   + ++ +TLI
Sbjct: 142 AGAMVLLVIGAGGIRPCNLPFGADQF-DPKTKEGKRGIESFFNWYFFTFTFAQMVSLTLI 201

Query: 210 VYIQDEFGWRTGYAVPAVLMLFSALIFLAGSSLYVKMKPAQSLFTGILQVIVVAFKNRRL 269
           VY+Q    W  G A+PA+LML   +IF AGS LYVK+K + S    I +VIVVA K RRL
Sbjct: 202 VYVQSNVSWSIGLAIPAILMLLGCIIFFAGSKLYVKVKASGSPIHSITRVIVVAIKKRRL 261

Query: 270 PLPYSNFDQYYLGHDPK--FLVPTDSMRCLNKACLIRDAENDLNPDGSVSNPWNLCSVDQ 329
                N    Y+  D K   L  T+  R L+K+  I+  ++ LN DGS  + W LCS+ Q
Sbjct: 262 KPVGPNELYNYIASDFKNSKLGHTEQFRFLDKSA-IQTQDDKLNKDGSPVDAWKLCSMQQ 321

Query: 330 VESLKNFLRIIPMW-STGIIMTVCVHQNSFGTLQAEIMNRHI-TGNFEIPAGSITVFMII 389
           VE +K  +R++P+W S  +     + Q ++   Q+   +R +  G+F+IPAGS TVF+++
Sbjct: 322 VEEVKCVIRVLPVWLSAALFYLAYIQQTTYTIFQSLQSDRRLGPGSFQIPAGSYTVFLML 381

Query: 390 SLSLWLVFYDRIIIPLLKR-CGHAGRLRPELRIGIGLLLSCFAMAISAVVEGTRRRIAIE 449
            +++++  YDR+++P L++  G  G +    R+G GL L   +M +SA+VE  RR++A+ 
Sbjct: 382 GMTIFIPIYDRVLVPFLRKYTGRDGGITQLQRVGAGLFLCITSMMVSAIVEQYRRKVALT 441

Query: 450 E---GLEDQPNAIIDMSALWIVPQCVVLGVAEGFNAVGQIEYFFLQIPKASSSMAVALHT 509
           +   GL  +  AI  MS +W++PQ V++G+A+    VGQ+E+++ Q P+   S A +L+ 
Sbjct: 442 KPTLGLAPRKGAISSMSGMWLIPQLVLMGIADALAGVGQMEFYYKQFPENMRSFAGSLYY 501

Query: 510 VEMSVANLVGSILVQIVNGITGKGNEPSWLDNNLNKGHLDYFYWLCAALALVNLCGYLLY 569
             + +A+ + + L+  V+  T   +  SWL  +LNKG L+YFY+L A +  +NL  +LL 
Sbjct: 502 CGIGLASYLSTFLLSAVHDTTEGFSGGSWLPEDLNKGRLEYFYFLVAGMMTLNLAYFLLV 561

Query: 570 NRAYGFSEKRQGNESSEIQNFEYRKL 587
           +  Y + +    ++  +  + E+ K+
Sbjct: 562 SHWYRYKDVVAKDKDMDKTSAEFDKV 585