MS024161.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS024161.1 vs. NCBI nr
Match: XP_022132097.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 [Momordica charantia])

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 586/589 (99.49%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           DDYGFQIAAASSILSLWSAASNALAIAGAVLADSY+GRFRSIILGSFSSFLGMALLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
           MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS LIFLVGSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALALMNFVGYLLCNRTYGLSENR+CNESNEVQDFEYRKLPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MS024161.1 vs. NCBI nr
Match: XP_022131895.1 (protein NRT1/ PTR FAMILY 1.2-like [Momordica charantia])

HSP 1 Score: 997.3 bits (2577), Expect = 5.7e-287
Identity = 493/589 (83.70%), Postives = 541/589 (91.85%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEVVEN EKTGIA+SS EGTHQASNCRTGGLRTMPFII NE FEKI+SLGL+PNM+FY R
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           D+YGFQIAAASS+LSLWSAASNAL+IAGAVLADSYMGRFR IILGSF SFLGMALLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
            IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGADQLTNEK  
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML SALIFL GSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+NQN+ G LQA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           K MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LSP LR+GIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAI+AVVE TRRRIA EEGLEDQP AV+DMS LW+VPQCVVLGVAEGFNA+GQI
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALAL+N  GYLL NR YG SE  Q NES+E+QDFEYR LPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MS024161.1 vs. NCBI nr
Match: XP_022132098.1 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 [Momordica charantia])

HSP 1 Score: 957.6 bits (2474), Expect = 5.0e-275
Identity = 476/478 (99.58%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 112 GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 171
           GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA
Sbjct: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79

Query: 172 DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 231
           DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS
Sbjct: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139

Query: 232 ALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 291
            LIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD
Sbjct: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199

Query: 292 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 351
           SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN
Sbjct: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259

Query: 352 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 411
           QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS
Sbjct: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319

Query: 412 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 471
           PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA
Sbjct: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 379

Query: 472 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 531
           EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD
Sbjct: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439

Query: 532 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENR+CNESNEVQDFEYRKLPSS
Sbjct: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 497

BLAST of MS024161.1 vs. NCBI nr
Match: XP_023545603.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 926.8 bits (2394), Expect = 9.4e-266
Identity = 466/589 (79.12%), Postives = 522/589 (88.62%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEV+E+ EKTGIA S SEG  QASN   GGL+TMPFIIVNETFE++ASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGIAVSGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           D +GFQIA AS ILSLWSAASNALAI GAVLADSY+GRF  IILGS SS LGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
           +IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGADQLT E+KP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           NN+ VLD+YFNWYYAS GIA++IAL+LIV+IQD+FGW  GFAVPAVLMLFS LIFLVGSS
Sbjct: 181 NNDSVLDSYFNWYYASIGIATIIALSLIVFIQDQFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCPIPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKD +N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQNSFGT+QA
Sbjct: 301 LIKDGKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           KTMNRHIT +FEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRTFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLS FAMAISA V   RRR+A E+G EDQP AVI+MS LW+VPQCVVLGVAE  N++GQ+
Sbjct: 421 LLSFFAMAISAGVAYIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYFYSQMPKTLSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALAL+NF GYL+C    G    RQ  E NE++DFEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALG----RQHKEPNEMEDFEYRNLPSS 585

BLAST of MS024161.1 vs. NCBI nr
Match: KAG6598782.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 926.4 bits (2393), Expect = 1.2e-265
Identity = 465/589 (78.95%), Postives = 522/589 (88.62%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEV+E+ EK+GIA S SEG  QASN   GGL+TMPFIIVNETFE++ASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKSGIAASGSEGAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           D +GF+IAAAS ILSLWSAASNALAI GAVLADSY+GRF  IILGS SS LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
           +IPQL+P SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGADQLT E+KP
Sbjct: 121 IIPQLRPESCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           NNE VLD+YFNWYYAS GIA+++AL+LIV+IQD+FGW  GFAVPAVLMLFS LIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIVALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDA+N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           KTMNRHIT SFEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAISA V   RRR+A E+G EDQP AVI+MS LW+VPQCVVLGVAE  N++GQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYFYSQMPKTLSSVAVALY+ EM VA+LVGS +VQMVN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMVVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALAL+NF GYL+C    G    RQ  E NE++DFEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALG----RQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024161.1 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.7e-156
Identity = 273/545 (50.09%), Postives = 389/545 (71.38%), Query Frame = 0

Query: 29  GGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAIAG 88
           GG+ TMPFII NE FEK+AS GL PNM+ YL  DY F +A  +++L +WSAASN   + G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSC--SSSGILCDSANPYQLA 148
           A L+DSY+GRF +I + S SSFLGM LLWLTAM+PQ+KPS C  +++G  C S+   QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 ILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALT 208
           +L+++   ISIG+G IRPCS+AFGADQL N++ P NE VL+++F WYYASS +A +IA T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 LIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNR 268
            IVYIQ+  GW+ GF VPAVLML +AL+F++ S LYV    T+SL TGL Q IV A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLSLP--YSNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSV 328
           +LSLP  + ++D YY   D +   P+  +R LNKACLI + E ++  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 EHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHIT---GSFEIPAGSIGVC 388
           + VE LK  +++IP+WSTGI+M +  +Q+SF  LQA +M+R ++    SF++PAGS G+ 
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MIISLSVWLAFYDRIMVPLLERC-GHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRI 448
            II+L++W+  YDR ++PL  +  G    LS +LRMG+GL +S  AMAISA+VE  RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 ATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYT 508
           A  +G  +   AV+D+S +W+VPQ V+ G+AE   AIGQ E+FY++ PK++SS+A +L+ 
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLC 566
           + MAVASL+ S ++  VN +T +  + SW+ +N+NKGH +Y+YW+ A ++ +N + Y++C
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of MS024161.1 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 528.1 bits (1359), Expect = 1.3e-148
Identity = 259/552 (46.92%), Postives = 382/552 (69.20%), Query Frame = 0

Query: 29  GGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAIAG 88
           GGL TMPFII NE FEK+AS GL  NM+ YL  DY   +    ++L +W AA+N + + G
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSC-SSSGILCDSANPYQLAI 148
           A L+DSY+GRF +I++ S SS LGM +LWLTAM+PQ+KPS C +++G  C SA   QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTL 208
           L+T+   ISIG+G IRPCS+AFGADQL N++ P NE VL+++F WYYASS +A +IA T+
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRR 268
           IVYIQD  GW+ GF +PA+LML +  +F+  S LYVK   ++SL TGL QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LSLP--YSNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVE 328
           L+LP  + + D YY   D +   P+D +R LNKAC I + + DL  DG   N W LC+ +
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 HVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHIT--GSFEIPAGSIGVCMI 388
            VE LK  +++IP+WSTGI+M + ++QNSF  LQAK+M+R ++   +F+IPAGS G+  I
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ISLSVWLAFYDRIMVPLLERC-GHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIAT 448
           I+L  W+  YDR ++PL  +  G    ++ ++RMG+GL +S  AMA+SA VE  RR+ A 
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVE 508
            +GL +   + + +S +W+VPQ V+ G+AE    IGQ E+FY++ PK++SS+A +L+ + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNR 568
           MAVA+++ S ++  V   + +GN  SW+++N+NKGH DY+YW+ A L+ +N + Y++C+ 
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

Query: 569 TYGLSENRQCNE 575
           +YG + ++  N+
Sbjct: 565 SYGPTVDQVRND 575

BLAST of MS024161.1 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 7.3e-112
Identity = 213/547 (38.94%), Postives = 331/547 (60.51%), Query Frame = 0

Query: 27  RTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAI 86
           + GG R + FI+ NET E++ S+GL  N + YL   +  +   A++++++WS  +N   +
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 87  AGAVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSCSSSGIL-CDSANPYQL 146
            GA ++D+Y+GRF++I   SF++ LG+  + LTA  PQL P+SC+S   L C   N  Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 147 AILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIAL 206
            +L   L F+S+G+G IRPCSI FG DQ     +   +GV  ++FNWYY +  +  +I  
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQ 230

Query: 207 TLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKN 266
           T++VYIQD+  W  GF++P  LM  + ++F  G   YV VKP  S+ +G+ QVIV A K 
Sbjct: 231 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 290

Query: 267 RRLSLPYSN------YDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPW 326
           R+L LP  +      YD          L  ++  RCL+KA ++   E DL P+G  ++ W
Sbjct: 291 RKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKW 350

Query: 327 NLCSVEHVESLKNFLRIIPMWSTGILMLVCL-NQNSFGTLQAKTMNRHITGSFEIPAGSI 386
            LCSV+ VE +K  +RI+P+WS GI+ L  +  Q +F   QA  M+R++   FEIPAGS+
Sbjct: 351 RLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSL 410

Query: 387 GVCMIISLSVWLAFYDRIMVPLLER-CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTR 446
            V  ++++ ++L FYDR+ VP + R  GH  G++   R+G G++ + F+M ++ +VE  R
Sbjct: 411 SVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMR 470

Query: 447 RRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVA 506
           R  +   G    P  +  MSV W+ PQ +++G+ E FN IGQIE+F SQ P+ + S+A +
Sbjct: 471 RIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANS 530

Query: 507 LYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGY 565
           L+++  A +S + S LV +V+  +G  +   WL+ NLN G LDYFY+L A L ++N V +
Sbjct: 531 LFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYF 590

BLAST of MS024161.1 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.3e-103
Identity = 223/573 (38.92%), Postives = 336/573 (58.64%), Query Frame = 0

Query: 4   VENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDY 63
           +E + K  I++   +  H   N   GGL TMPFI  NE  EK+A +G   NM+ YL    
Sbjct: 1   MEEQSKNKISEEEKQ-LHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQL 60

Query: 64  GFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIP 123
              +  A++ L+ ++  S+   + GA +ADS+ GRF +I   S    +GM LL ++A+IP
Sbjct: 61  HLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIP 120

Query: 124 QLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNE 183
            L+P  C    + C  A+  QL+IL+ +L   ++G+G IRPC +AFGADQ  +E  PN  
Sbjct: 121 TLRPPPCKGEEV-CVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQT 180

Query: 184 GVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYV 243
                YFNWYY   G A ++A+T++V+IQD  GW  G  +P V M  S + F+ G  LY 
Sbjct: 181 TKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYR 240

Query: 244 KVKPTQSLLTGLLQVIVVAFKNRRL------SLPYSNYD---QYYLGPDPKFLVPTDSMR 303
            + P  S  T L+QV V AF+ R+L      SL Y N +      LG     L  T  M 
Sbjct: 241 HLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMS 300

Query: 304 CLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVC-LNQN 363
            L+KA ++ + E++L P G I N W L +V  VE LK+ +R+ P+ ++GIL++     Q 
Sbjct: 301 FLDKAAIVTE-EDNLKP-GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQG 360

Query: 364 SFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLER-CGHAGGLSP 423
           +F   QAKTMNRH+T SF+IPAGS+ V   +++   + FYDR+ V +  +  G   G++ 
Sbjct: 361 TFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITF 420

Query: 424 RLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAE 483
             RMGIG ++S  A  ++  VE  R+ +A E GL D+P+ ++ +S LW++PQ  + GVAE
Sbjct: 421 LHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAE 480

Query: 484 GFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWL-D 543
            F +IG +E+FY Q P+++ S A AL+ + +++ + V + LV +V+  + K + ++WL D
Sbjct: 481 AFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPD 540

Query: 544 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTY 565
           NNLN+G L+YFYWL   L  +N V YL C + Y
Sbjct: 541 NNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIY 565

BLAST of MS024161.1 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 369.4 bits (947), Expect = 7.6e-101
Identity = 212/559 (37.92%), Postives = 326/559 (58.32%), Query Frame = 0

Query: 27  RTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAI 86
           + GG R + FI+ NET EK+ S+G++ N + YLR+ +  +   A ++  LW   +N   +
Sbjct: 14  KLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPL 73

Query: 87  AGAVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSCSS-SGILCDSANPYQL 146
            GA+++D+Y+GRF++I   S  S LG+  + LTA +PQL P  C++     CD  N  QL
Sbjct: 74  LGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQL 133

Query: 147 AILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIAL 206
            ILF  LGF+SIG+G IRPCSI FG DQ     +   +GV  ++FNWYY +  +  + + 
Sbjct: 134 GILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVA-SFFNWYYLTLTMVLIFSH 193

Query: 207 TLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKN 266
           T++VY+Q    W  GF++P  LM  + ++F VG   YV VKP  S+ +G+ +VIV A K 
Sbjct: 194 TVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKK 253

Query: 267 R--RLSLPYSNYDQYYLGP-DPKFL--VP-TDSMRCLNKACLIKDAENDLNPDGSISNPW 326
           R  ++SL     ++YY  P  P  L  +P TD  + L+KA +I D   DL  +G  +N W
Sbjct: 254 RDLKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILD--GDLTSEGVPANKW 313

Query: 327 NLCSVEHVESLKNFLRIIPMWSTGILMLVCL-NQNSFGTLQAKTMNRHITGSFEIPAGSI 386
            LCS++ VE +K  +R++P+WS GI+ +V +  Q +F   QA  M+RH+   FEIPA SI
Sbjct: 314 RLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASI 373

Query: 387 GVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRR 446
            V   I++ +W+  Y+ ++VP L R      ++   RMGIG++ +  +M  +  VEG RR
Sbjct: 374 TVISYITIGIWVPIYEHLLVPFLWRM-RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRR 433

Query: 447 RIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVAL 506
             ATE         +  MSV W+    +++G+ E FN IG IE+F SQ P+ + S+A +L
Sbjct: 434 TRATE---------MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSL 493

Query: 507 YTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYL 566
           + +  A A+ + S LV  V+ ++G  +   WL+ +L++G LDYFY+L A L ++N V + 
Sbjct: 494 FPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYFW 553

Query: 567 LCNRTYGLSENRQCNESNE 578
            C   Y      Q  + NE
Sbjct: 554 YCAHRYQYKAGSQIEDFNE 558

BLAST of MS024161.1 vs. ExPASy TrEMBL
Match: A0A6J1BSW7 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 1161.4 bits (3003), Expect = 0.0e+00
Identity = 586/589 (99.49%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR
Sbjct: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           DDYGFQIAAASSILSLWSAASNALAIAGAVLADSY+GRFRSIILGSFSSFLGMALLWLTA
Sbjct: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYVGRFRSIILGSFSSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
           MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP
Sbjct: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS LIFLVGSS
Sbjct: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA
Sbjct: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL
Sbjct: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI
Sbjct: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALALMNFVGYLLCNRTYGLSENR+CNESNEVQDFEYRKLPSS
Sbjct: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 589

BLAST of MS024161.1 vs. ExPASy TrEMBL
Match: A0A6J1BQY7 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111004925 PE=3 SV=1)

HSP 1 Score: 997.3 bits (2577), Expect = 2.7e-287
Identity = 493/589 (83.70%), Postives = 541/589 (91.85%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEVVEN EKTGIA+SS EGTHQASNCRTGGLRTMPFII NE FEKI+SLGL+PNM+FY R
Sbjct: 1   MEVVENVEKTGIAKSSCEGTHQASNCRTGGLRTMPFIIANEVFEKISSLGLSPNMMFYFR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           D+YGFQIAAASS+LSLWSAASNAL+IAGAVLADSYMGRFR IILGSF SFLGMALLWLTA
Sbjct: 61  DEYGFQIAAASSVLSLWSAASNALSIAGAVLADSYMGRFRVIILGSFCSFLGMALLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
            IPQLKPSSCSSSGILCDSAN YQLA+LFTSLGFISIGAGCI+PCS+AFGADQLTNEK  
Sbjct: 121 TIPQLKPSSCSSSGILCDSANLYQLAVLFTSLGFISIGAGCIKPCSMAFGADQLTNEKNS 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           +N+GVLDTYFNWYYA+SGI++VI +T+IVYIQDEFGW TG+ VPAVLML SALIFL GSS
Sbjct: 181 SNKGVLDTYFNWYYAASGISTVIGMTVIVYIQDEFGWGTGYVVPAVLMLLSALIFLAGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKP QSL TG+ QVIVVAFKNRRLSLPYSN+DQYYLG DPK LVPTDSMRCLNKAC
Sbjct: 241 LYVKVKPAQSLFTGIFQVIVVAFKNRRLSLPYSNFDQYYLGHDPKLLVPTDSMRCLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDAENDLNPDGS+SNPWNLCSV+ VESLKNF+RIIP+WSTGI+M+VC+NQN+ G LQA
Sbjct: 301 LIKDAENDLNPDGSVSNPWNLCSVDQVESLKNFIRIIPVWSTGIIMVVCINQNALGALQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           K MNRHITGSFE PAGSI V MIISLS+WL FYDRI++PLL+RCGHAG LSP LR+GIGL
Sbjct: 361 KIMNRHITGSFEFPAGSINVFMIISLSLWLVFYDRIIMPLLKRCGHAGRLSPELRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAI+AVVE TRRRIA EEGLEDQP AV+DMS LW+VPQCVVLGVAEGFNA+GQI
Sbjct: 421 LLSCFAMAIAAVVEATRRRIAIEEGLEDQPNAVVDMSALWLVPQCVVLGVAEGFNAVGQI 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYF+S++ K  SS+AVAL+TVEM+VA+LVGS LVQ+VNGITGKGNETSWLDNNLNKGHLD
Sbjct: 481 EYFFSKISKVSSSMAVALHTVEMSVANLVGSILVQIVNGITGKGNETSWLDNNLNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALAL+N  GYLL NR YG SE  Q NES+E+QDFEYR LPSS
Sbjct: 541 YFYWLCAALALVNLCGYLLYNRAYGFSEKIQGNESSEIQDFEYRNLPSS 589

BLAST of MS024161.1 vs. ExPASy TrEMBL
Match: A0A6J1BVB4 (protein NRT1/ PTR FAMILY 1.2-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005055 PE=3 SV=1)

HSP 1 Score: 957.6 bits (2474), Expect = 2.4e-275
Identity = 476/478 (99.58%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 112 GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 171
           GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA
Sbjct: 20  GMALLWLTAMIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGA 79

Query: 172 DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 231
           DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS
Sbjct: 80  DQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFS 139

Query: 232 ALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 291
            LIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD
Sbjct: 140 VLIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTD 199

Query: 292 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 351
           SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN
Sbjct: 200 SMRCLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLN 259

Query: 352 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 411
           QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS
Sbjct: 260 QNSFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLS 319

Query: 412 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 471
           PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA
Sbjct: 320 PRLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVA 379

Query: 472 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 531
           EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD
Sbjct: 380 EGFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLD 439

Query: 532 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENR+CNESNEVQDFEYRKLPSS
Sbjct: 440 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTYGLSENRRCNESNEVQDFEYRKLPSS 497

BLAST of MS024161.1 vs. ExPASy TrEMBL
Match: A0A6J1K947 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491783 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.3e-265
Identity = 466/589 (79.12%), Postives = 522/589 (88.62%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEV+E+ EKTGI+ S SEGT QASN   GGL+TMPFIIVNETFE++ASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGISASGSEGTEQASNHTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           D +GFQIA AS ILSLWSAASNALAI GAVLADSY+GRF  IILGS SS LGM LLWLTA
Sbjct: 61  DVFGFQIADASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
           +IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGADQLT E+KP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           NNE VLD+YFNWYYAS GIA++ AL+LIV+IQD+FGW  GFAVPAVLMLFS LIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIFALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGCDPKCPIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDA+N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           KTMNRHIT SFEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARYGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAISA V   RRR+A E+G EDQP AVI+MS LW+VPQCVVLGVAE  N++GQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYFYSQMPK LSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKMLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALAL+NF GYL+C    G    RQ  E NE++DFEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLVCCWALG----RQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024161.1 vs. ExPASy TrEMBL
Match: A0A6J1HEG1 (protein NRT1/ PTR FAMILY 1.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462798 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 3.0e-265
Identity = 466/589 (79.12%), Postives = 523/589 (88.79%), Query Frame = 0

Query: 1   MEVVENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLR 60
           MEV+E+ EKTGIA S SE   QASN   GGL+TMPFIIVNETFE++ASLGLTPNMVFYLR
Sbjct: 1   MEVLEDGEKTGIAASGSEEAEQASNYTNGGLKTMPFIIVNETFERVASLGLTPNMVFYLR 60

Query: 61  DDYGFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTA 120
           D +GF+IAAAS ILSLWSAASNALAI GAVLADSY+GRF  IILGS SS LGM LLWLTA
Sbjct: 61  DVFGFEIAAASIILSLWSAASNALAIVGAVLADSYLGRFCVIILGSCSSLLGMILLWLTA 120

Query: 121 MIPQLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKP 180
           +IPQL+P+SCS SGILCDSANPYQLA+LF+SL FISIGAGCIRPCS+AFGADQLT E+KP
Sbjct: 121 IIPQLRPASCSPSGILCDSANPYQLAVLFSSLVFISIGAGCIRPCSVAFGADQLTKEEKP 180

Query: 181 NNEGVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSS 240
           NNE VLD+YFNWYYAS GIA++IAL+LIV+IQD+FGW  GFAVPAVLMLFS LIFLVGSS
Sbjct: 181 NNESVLDSYFNWYYASIGIATIIALSLIVFIQDKFGWGIGFAVPAVLMLFSVLIFLVGSS 240

Query: 241 LYVKVKPTQSLLTGLLQVIVVAFKNRRLSLPYSNYDQYYLGPDPKFLVPTDSMRCLNKAC 300
           LYVKVKPTQSLLTG L+VIVVAFKNR+LSLP+SN+DQYYLG DPK  +PT+SMR LNKAC
Sbjct: 241 LYVKVKPTQSLLTGFLRVIVVAFKNRKLSLPFSNFDQYYLGRDPKCRIPTNSMRYLNKAC 300

Query: 301 LIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQA 360
           LIKDA+N+LNPDGS+SNPWNLCSV+ VESLK FLRIIPMWSTGILMLVCLNQNSFGT+QA
Sbjct: 301 LIKDAKNNLNPDGSVSNPWNLCSVDQVESLKAFLRIIPMWSTGILMLVCLNQNSFGTIQA 360

Query: 361 KTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGL 420
           KTMNRHIT SFEIPAGSI + M++SL  W+ FYDRI+VPLL R GHAGGLSP+LR+GIGL
Sbjct: 361 KTMNRHITRSFEIPAGSITIFMVLSLFSWIMFYDRILVPLLARHGHAGGLSPKLRIGIGL 420

Query: 421 LLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQI 480
           LLSCFAMAISA V   RRR+A E+G EDQP AVI+MS LW+VPQCVVLGVAE  N++GQ+
Sbjct: 421 LLSCFAMAISAGVACIRRRVAIEQGFEDQPNAVINMSALWMVPQCVVLGVAEALNSVGQV 480

Query: 481 EYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLD 540
           EYFYSQMPKTLSSVAVALY+ EMAVA+LVGS +VQMVN ITG+GN+TSWLDNN+NKGHLD
Sbjct: 481 EYFYSQMPKTLSSVAVALYSFEMAVANLVGSIIVQMVNSITGEGNKTSWLDNNVNKGHLD 540

Query: 541 YFYWLCAALALMNFVGYLLCNRTYGLSENRQCNESNEVQDFEYRKLPSS 590
           YFYWLCAALAL+NF GYL+    Y  +  RQ  E NE++DFEYR LPSS
Sbjct: 541 YFYWLCAALALINFFGYLV----YCWALGRQDKEPNEMEDFEYRNLPSS 585

BLAST of MS024161.1 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.6e-157
Identity = 273/545 (50.09%), Postives = 389/545 (71.38%), Query Frame = 0

Query: 29  GGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAIAG 88
           GG+ TMPFII NE FEK+AS GL PNM+ YL  DY F +A  +++L +WSAASN   + G
Sbjct: 23  GGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLG 82

Query: 89  AVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSC--SSSGILCDSANPYQLA 148
           A L+DSY+GRF +I + S SSFLGM LLWLTAM+PQ+KPS C  +++G  C S+   QLA
Sbjct: 83  AFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLA 142

Query: 149 ILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALT 208
           +L+++   ISIG+G IRPCS+AFGADQL N++ P NE VL+++F WYYASS +A +IA T
Sbjct: 143 LLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFT 202

Query: 209 LIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNR 268
            IVYIQ+  GW+ GF VPAVLML +AL+F++ S LYV    T+SL TGL Q IV A+K R
Sbjct: 203 GIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKR 262

Query: 269 RLSLP--YSNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSV 328
           +LSLP  + ++D YY   D +   P+  +R LNKACLI + E ++  DG   NPW LC+ 
Sbjct: 263 KLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTT 322

Query: 329 EHVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHIT---GSFEIPAGSIGVC 388
           + VE LK  +++IP+WSTGI+M +  +Q+SF  LQA +M+R ++    SF++PAGS G+ 
Sbjct: 323 DKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMF 382

Query: 389 MIISLSVWLAFYDRIMVPLLERC-GHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRI 448
            II+L++W+  YDR ++PL  +  G    LS +LRMG+GL +S  AMAISA+VE  RR+ 
Sbjct: 383 TIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKK 442

Query: 449 ATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYT 508
           A  +G  +   AV+D+S +W+VPQ V+ G+AE   AIGQ E+FY++ PK++SS+A +L+ 
Sbjct: 443 AISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFG 502

Query: 509 VEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLC 566
           + MAVASL+ S ++  VN +T +  + SW+ +N+NKGH +Y+YW+ A ++ +N + Y++C
Sbjct: 503 LGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVIC 562

BLAST of MS024161.1 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 528.1 bits (1359), Expect = 9.1e-150
Identity = 259/552 (46.92%), Postives = 382/552 (69.20%), Query Frame = 0

Query: 29  GGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAIAG 88
           GGL TMPFII NE FEK+AS GL  NM+ YL  DY   +    ++L +W AA+N + + G
Sbjct: 25  GGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVG 84

Query: 89  AVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSC-SSSGILCDSANPYQLAI 148
           A L+DSY+GRF +I++ S SS LGM +LWLTAM+PQ+KPS C +++G  C SA   QLA+
Sbjct: 85  AFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLAL 144

Query: 149 LFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIALTL 208
           L+T+   ISIG+G IRPCS+AFGADQL N++ P NE VL+++F WYYASS +A +IA T+
Sbjct: 145 LYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTV 204

Query: 209 IVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKNRR 268
           IVYIQD  GW+ GF +PA+LML +  +F+  S LYVK   ++SL TGL QV+  A+  R 
Sbjct: 205 IVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRN 264

Query: 269 LSLP--YSNYDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPWNLCSVE 328
           L+LP  + + D YY   D +   P+D +R LNKAC I + + DL  DG   N W LC+ +
Sbjct: 265 LTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTD 324

Query: 329 HVESLKNFLRIIPMWSTGILMLVCLNQNSFGTLQAKTMNRHIT--GSFEIPAGSIGVCMI 388
            VE LK  +++IP+WSTGI+M + ++QNSF  LQAK+M+R ++   +F+IPAGS G+  I
Sbjct: 325 QVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTI 384

Query: 389 ISLSVWLAFYDRIMVPLLERC-GHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRRRIAT 448
           I+L  W+  YDR ++PL  +  G    ++ ++RMG+GL +S  AMA+SA VE  RR+ A 
Sbjct: 385 IALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAI 444

Query: 449 EEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVALYTVE 508
            +GL +   + + +S +W+VPQ V+ G+AE    IGQ E+FY++ PK++SS+A +L+ + 
Sbjct: 445 SQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLG 504

Query: 509 MAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYLLCNR 568
           MAVA+++ S ++  V   + +GN  SW+++N+NKGH DY+YW+ A L+ +N + Y++C+ 
Sbjct: 505 MAVANILASVILNAVKNSSKQGN-VSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSW 564

Query: 569 TYGLSENRQCNE 575
           +YG + ++  N+
Sbjct: 565 SYGPTVDQVRND 575

BLAST of MS024161.1 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 406.0 bits (1042), Expect = 5.2e-113
Identity = 213/547 (38.94%), Postives = 331/547 (60.51%), Query Frame = 0

Query: 27  RTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAI 86
           + GG R + FI+ NET E++ S+GL  N + YL   +  +   A++++++WS  +N   +
Sbjct: 51  KPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPL 110

Query: 87  AGAVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSCSSSGIL-CDSANPYQL 146
            GA ++D+Y+GRF++I   SF++ LG+  + LTA  PQL P+SC+S   L C   N  Q+
Sbjct: 111 VGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQI 170

Query: 147 AILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIAL 206
            +L   L F+S+G+G IRPCSI FG DQ     +   +GV  ++FNWYY +  +  +I  
Sbjct: 171 GVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVA-SFFNWYYMTFTVVLIITQ 230

Query: 207 TLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKN 266
           T++VYIQD+  W  GF++P  LM  + ++F  G   YV VKP  S+ +G+ QVIV A K 
Sbjct: 231 TVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKK 290

Query: 267 RRLSLPYSN------YDQYYLGPDPKFLVPTDSMRCLNKACLIKDAENDLNPDGSISNPW 326
           R+L LP  +      YD          L  ++  RCL+KA ++   E DL P+G  ++ W
Sbjct: 291 RKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKW 350

Query: 327 NLCSVEHVESLKNFLRIIPMWSTGILMLVCL-NQNSFGTLQAKTMNRHITGSFEIPAGSI 386
            LCSV+ VE +K  +RI+P+WS GI+ L  +  Q +F   QA  M+R++   FEIPAGS+
Sbjct: 351 RLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSL 410

Query: 387 GVCMIISLSVWLAFYDRIMVPLLER-CGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTR 446
            V  ++++ ++L FYDR+ VP + R  GH  G++   R+G G++ + F+M ++ +VE  R
Sbjct: 411 SVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMR 470

Query: 447 RRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVA 506
           R  +   G    P  +  MSV W+ PQ +++G+ E FN IGQIE+F SQ P+ + S+A +
Sbjct: 471 RIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANS 530

Query: 507 LYTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGY 565
           L+++  A +S + S LV +V+  +G  +   WL+ NLN G LDYFY+L A L ++N V +
Sbjct: 531 LFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYF 590

BLAST of MS024161.1 vs. TAIR 10
Match: AT1G68570.1 (Major facilitator superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 8.9e-105
Identity = 223/573 (38.92%), Postives = 336/573 (58.64%), Query Frame = 0

Query: 4   VENEEKTGIAQSSSEGTHQASNCRTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDY 63
           +E + K  I++   +  H   N   GGL TMPFI  NE  EK+A +G   NM+ YL    
Sbjct: 1   MEEQSKNKISEEEKQ-LHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQL 60

Query: 64  GFQIAAASSILSLWSAASNALAIAGAVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIP 123
              +  A++ L+ ++  S+   + GA +ADS+ GRF +I   S    +GM LL ++A+IP
Sbjct: 61  HLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIP 120

Query: 124 QLKPSSCSSSGILCDSANPYQLAILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNE 183
            L+P  C    + C  A+  QL+IL+ +L   ++G+G IRPC +AFGADQ  +E  PN  
Sbjct: 121 TLRPPPCKGEEV-CVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQF-DESDPNQT 180

Query: 184 GVLDTYFNWYYASSGIASVIALTLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYV 243
                YFNWYY   G A ++A+T++V+IQD  GW  G  +P V M  S + F+ G  LY 
Sbjct: 181 TKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYR 240

Query: 244 KVKPTQSLLTGLLQVIVVAFKNRRL------SLPYSNYD---QYYLGPDPKFLVPTDSMR 303
            + P  S  T L+QV V AF+ R+L      SL Y N +      LG     L  T  M 
Sbjct: 241 HLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGK---LTHTKHMS 300

Query: 304 CLNKACLIKDAENDLNPDGSISNPWNLCSVEHVESLKNFLRIIPMWSTGILMLVC-LNQN 363
            L+KA ++ + E++L P G I N W L +V  VE LK+ +R+ P+ ++GIL++     Q 
Sbjct: 301 FLDKAAIVTE-EDNLKP-GQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQG 360

Query: 364 SFGTLQAKTMNRHITGSFEIPAGSIGVCMIISLSVWLAFYDRIMVPLLER-CGHAGGLSP 423
           +F   QAKTMNRH+T SF+IPAGS+ V   +++   + FYDR+ V +  +  G   G++ 
Sbjct: 361 TFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITF 420

Query: 424 RLRMGIGLLLSCFAMAISAVVEGTRRRIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAE 483
             RMGIG ++S  A  ++  VE  R+ +A E GL D+P+ ++ +S LW++PQ  + GVAE
Sbjct: 421 LHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAE 480

Query: 484 GFNAIGQIEYFYSQMPKTLSSVAVALYTVEMAVASLVGSALVQMVNGITGKGNETSWL-D 543
            F +IG +E+FY Q P+++ S A AL+ + +++ + V + LV +V+  + K + ++WL D
Sbjct: 481 AFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPD 540

Query: 544 NNLNKGHLDYFYWLCAALALMNFVGYLLCNRTY 565
           NNLN+G L+YFYWL   L  +N V YL C + Y
Sbjct: 541 NNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIY 565

BLAST of MS024161.1 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 369.4 bits (947), Expect = 5.4e-102
Identity = 212/559 (37.92%), Postives = 326/559 (58.32%), Query Frame = 0

Query: 27  RTGGLRTMPFIIVNETFEKIASLGLTPNMVFYLRDDYGFQIAAASSILSLWSAASNALAI 86
           + GG R + FI+ NET EK+ S+G++ N + YLR+ +  +   A ++  LW   +N   +
Sbjct: 14  KLGGWRAITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPL 73

Query: 87  AGAVLADSYMGRFRSIILGSFSSFLGMALLWLTAMIPQLKPSSCSS-SGILCDSANPYQL 146
            GA+++D+Y+GRF++I   S  S LG+  + LTA +PQL P  C++     CD  N  QL
Sbjct: 74  LGALISDAYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQL 133

Query: 147 AILFTSLGFISIGAGCIRPCSIAFGADQLTNEKKPNNEGVLDTYFNWYYASSGIASVIAL 206
            ILF  LGF+SIG+G IRPCSI FG DQ     +   +GV  ++FNWYY +  +  + + 
Sbjct: 134 GILFLGLGFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVA-SFFNWYYLTLTMVLIFSH 193

Query: 207 TLIVYIQDEFGWRTGFAVPAVLMLFSALIFLVGSSLYVKVKPTQSLLTGLLQVIVVAFKN 266
           T++VY+Q    W  GF++P  LM  + ++F VG   YV VKP  S+ +G+ +VIV A K 
Sbjct: 194 TVVVYLQ-TVSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKK 253

Query: 267 R--RLSLPYSNYDQYYLGP-DPKFL--VP-TDSMRCLNKACLIKDAENDLNPDGSISNPW 326
           R  ++SL     ++YY  P  P  L  +P TD  + L+KA +I D   DL  +G  +N W
Sbjct: 254 RDLKISLVDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILD--GDLTSEGVPANKW 313

Query: 327 NLCSVEHVESLKNFLRIIPMWSTGILMLVCL-NQNSFGTLQAKTMNRHITGSFEIPAGSI 386
            LCS++ VE +K  +R++P+WS GI+ +V +  Q +F   QA  M+RH+   FEIPA SI
Sbjct: 314 RLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASI 373

Query: 387 GVCMIISLSVWLAFYDRIMVPLLERCGHAGGLSPRLRMGIGLLLSCFAMAISAVVEGTRR 446
            V   I++ +W+  Y+ ++VP L R      ++   RMGIG++ +  +M  +  VEG RR
Sbjct: 374 TVISYITIGIWVPIYEHLLVPFLWRM-RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRR 433

Query: 447 RIATEEGLEDQPYAVIDMSVLWIVPQCVVLGVAEGFNAIGQIEYFYSQMPKTLSSVAVAL 506
             ATE         +  MSV W+    +++G+ E FN IG IE+F SQ P+ + S+A +L
Sbjct: 434 TRATE---------MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSL 493

Query: 507 YTVEMAVASLVGSALVQMVNGITGKGNETSWLDNNLNKGHLDYFYWLCAALALMNFVGYL 566
           + +  A A+ + S LV  V+ ++G  +   WL+ +L++G LDYFY+L A L ++N V + 
Sbjct: 494 FPLSFAAANYLSSLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYFW 553

Query: 567 LCNRTYGLSENRQCNESNE 578
            C   Y      Q  + NE
Sbjct: 554 YCAHRYQYKAGSQIEDFNE 558