MS022987.1 (mRNA) Bitter gourd (TR) v1

Overview
NameMS022987.1
TypemRNA
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRING-type domain-containing protein
Locationscaffold357: 350008 .. 351279 (-)
Sequence length1272
RNA-Seq ExpressionMS022987.1
SyntenyMS022987.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAATGGATGAAAGCGCTGAAGCAATGAATCTTGACTTGAATCTGGGTCCTATTCCAGAGCCACTTTCAAACTCGATTCCGAATGAAGAGGAAAATTTAGCTTATTTGACTGAAGACCCTGTTCGTAGATTTGGGGAAGCTATCAGGTTTAGGACGAGACAGCGGTGGAGGAGGCGTCAAGTCCAAATTCCTCCTGAGACTCAGAACATTTCTATGCAATTGAGTGAAATTATGGTCAATTCAGGCAATGGGAATTCATTACAGCCGTTACAGGCTGGTGAGGGTAGTACTACTGCTGAGGAAAGGAAAAATGAGGCAGGGAAAACATGTGAAATCAACAATGAAGCTTTGGAAGATGAAAAGGCAGAGAATAAGAGTGATGTTGAGAAGAGCAGTGATTCTGATGGGAGCTTTTTTGATTGCAATATATGTCTGGACTTGGCTAGGGACCCTGTTGTAACTTGTTGTGGTCATCTATACTGCTGGCCATGTCTTTATCGATGGTTACATCTACATTCAGAGGCCAAGGAATGTCCTGTATGTAAAGGTGAGGTTACTACGAAGAACGTGACTCCAATTTATGGGCGTGGGAGCAATACCCCTGTGACAGAGGAGGATGCTACACTTAAGATTCCTCTAAGGCCTCATGCCCGGAAGGTCGAAAGTTTGAGGCAAACCATCCAGAGGACTGCATTTTCTTTCCCAGTGGAGGAGATGATCCGCCGTTTGGGGAACAGATTTGATTTCAATCGTGACTTAGACCAACCACCAGAGCCAGATAGTTCACACGAAACATTTGGTAGATCTCCAACCTTGTTGAATAGGATTTTGACATCCAGGGGAATGCGTAGAGAACAAATTTCATTGCCGCCCCATGATGATGTGGGAGATCTAGCACACGCTGATGTGTCTGGCATTGAGGCAGGGGAAACCCGTCTTCAGTCTCTCCCAGTACTTCGATCCTTACTACACCGGACAAGAGTTTCTTCTCTTACTTCTGCATTTAATTCTGCTGATAGGCTTCCAGATGGTTTTATATATACCGATCCACTCGTTCCTAGAAATCAAGAGCAGCCTCCACCAGTCGAGGACAGAGATTCTTTCTCTAGCATTGCTGCTGTTATAAATTCAGAGAGTCAAATGGACACTGCTGTAGAAATTGATTCCATGGTCTCCCTTTCAACATCATCATCCAGAAGGAGAAATGATGCTCTACAGGTTTCTGATGTAGACAGTGGAAACTCTCGGGCACCCAGACGGAGAAGATTGGCT

mRNA sequence

CTAATGGATGAAAGCGCTGAAGCAATGAATCTTGACTTGAATCTGGGTCCTATTCCAGAGCCACTTTCAAACTCGATTCCGAATGAAGAGGAAAATTTAGCTTATTTGACTGAAGACCCTGTTCGTAGATTTGGGGAAGCTATCAGGTTTAGGACGAGACAGCGGTGGAGGAGGCGTCAAGTCCAAATTCCTCCTGAGACTCAGAACATTTCTATGCAATTGAGTGAAATTATGGTCAATTCAGGCAATGGGAATTCATTACAGCCGTTACAGGCTGGTGAGGGTAGTACTACTGCTGAGGAAAGGAAAAATGAGGCAGGGAAAACATGTGAAATCAACAATGAAGCTTTGGAAGATGAAAAGGCAGAGAATAAGAGTGATGTTGAGAAGAGCAGTGATTCTGATGGGAGCTTTTTTGATTGCAATATATGTCTGGACTTGGCTAGGGACCCTGTTGTAACTTGTTGTGGTCATCTATACTGCTGGCCATGTCTTTATCGATGGTTACATCTACATTCAGAGGCCAAGGAATGTCCTGTATGTAAAGGTGAGGTTACTACGAAGAACGTGACTCCAATTTATGGGCGTGGGAGCAATACCCCTGTGACAGAGGAGGATGCTACACTTAAGATTCCTCTAAGGCCTCATGCCCGGAAGGTCGAAAGTTTGAGGCAAACCATCCAGAGGACTGCATTTTCTTTCCCAGTGGAGGAGATGATCCGCCGTTTGGGGAACAGATTTGATTTCAATCGTGACTTAGACCAACCACCAGAGCCAGATAGTTCACACGAAACATTTGGTAGATCTCCAACCTTGTTGAATAGGATTTTGACATCCAGGGGAATGCGTAGAGAACAAATTTCATTGCCGCCCCATGATGATGTGGGAGATCTAGCACACGCTGATGTGTCTGGCATTGAGGCAGGGGAAACCCGTCTTCAGTCTCTCCCAGTACTTCGATCCTTACTACACCGGACAAGAGTTTCTTCTCTTACTTCTGCATTTAATTCTGCTGATAGGCTTCCAGATGGTTTTATATATACCGATCCACTCGTTCCTAGAAATCAAGAGCAGCCTCCACCAGTCGAGGACAGAGATTCTTTCTCTAGCATTGCTGCTGTTATAAATTCAGAGAGTCAAATGGACACTGCTGTAGAAATTGATTCCATGGTCTCCCTTTCAACATCATCATCCAGAAGGAGAAATGATGCTCTACAGGTTTCTGATGTAGACAGTGGAAACTCTCGGGCACCCAGACGGAGAAGATTGGCT

Coding sequence (CDS)

CTAATGGATGAAAGCGCTGAAGCAATGAATCTTGACTTGAATCTGGGTCCTATTCCAGAGCCACTTTCAAACTCGATTCCGAATGAAGAGGAAAATTTAGCTTATTTGACTGAAGACCCTGTTCGTAGATTTGGGGAAGCTATCAGGTTTAGGACGAGACAGCGGTGGAGGAGGCGTCAAGTCCAAATTCCTCCTGAGACTCAGAACATTTCTATGCAATTGAGTGAAATTATGGTCAATTCAGGCAATGGGAATTCATTACAGCCGTTACAGGCTGGTGAGGGTAGTACTACTGCTGAGGAAAGGAAAAATGAGGCAGGGAAAACATGTGAAATCAACAATGAAGCTTTGGAAGATGAAAAGGCAGAGAATAAGAGTGATGTTGAGAAGAGCAGTGATTCTGATGGGAGCTTTTTTGATTGCAATATATGTCTGGACTTGGCTAGGGACCCTGTTGTAACTTGTTGTGGTCATCTATACTGCTGGCCATGTCTTTATCGATGGTTACATCTACATTCAGAGGCCAAGGAATGTCCTGTATGTAAAGGTGAGGTTACTACGAAGAACGTGACTCCAATTTATGGGCGTGGGAGCAATACCCCTGTGACAGAGGAGGATGCTACACTTAAGATTCCTCTAAGGCCTCATGCCCGGAAGGTCGAAAGTTTGAGGCAAACCATCCAGAGGACTGCATTTTCTTTCCCAGTGGAGGAGATGATCCGCCGTTTGGGGAACAGATTTGATTTCAATCGTGACTTAGACCAACCACCAGAGCCAGATAGTTCACACGAAACATTTGGTAGATCTCCAACCTTGTTGAATAGGATTTTGACATCCAGGGGAATGCGTAGAGAACAAATTTCATTGCCGCCCCATGATGATGTGGGAGATCTAGCACACGCTGATGTGTCTGGCATTGAGGCAGGGGAAACCCGTCTTCAGTCTCTCCCAGTACTTCGATCCTTACTACACCGGACAAGAGTTTCTTCTCTTACTTCTGCATTTAATTCTGCTGATAGGCTTCCAGATGGTTTTATATATACCGATCCACTCGTTCCTAGAAATCAAGAGCAGCCTCCACCAGTCGAGGACAGAGATTCTTTCTCTAGCATTGCTGCTGTTATAAATTCAGAGAGTCAAATGGACACTGCTGTAGAAATTGATTCCATGGTCTCCCTTTCAACATCATCATCCAGAAGGAGAAATGATGCTCTACAGGTTTCTGATGTAGACAGTGGAAACTCTCGGGCACCCAGACGGAGAAGATTGGCT

Protein sequence

LMDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQVQIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEKAENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIRRLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHADVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRRRLA
Homology
BLAST of MS022987.1 vs. NCBI nr
Match: XP_022151415.1 (uncharacterized protein LOC111019352 [Momordica charantia])

HSP 1 Score: 833.6 bits (2152), Expect = 7.8e-238
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 2   MDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 61
           MDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV
Sbjct: 1   MDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 60

Query: 62  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 121
           QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK
Sbjct: 61  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 120

Query: 122 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 181
           AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC
Sbjct: 121 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 180

Query: 182 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 241
           KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR
Sbjct: 181 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 240

Query: 242 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 301
           RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA
Sbjct: 241 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 300

Query: 302 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPP 361
           DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPP
Sbjct: 301 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPP 360

Query: 362 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 421
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR
Sbjct: 361 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 420

Query: 422 RLA 425
           RLA
Sbjct: 421 RLA 423

BLAST of MS022987.1 vs. NCBI nr
Match: XP_038904860.1 (uncharacterized protein LOC120091094 isoform X1 [Benincasa hispida])

HSP 1 Score: 780.0 bits (2013), Expect = 1.0e-221
Identity = 395/423 (93.38%), Postives = 410/423 (96.93%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQVQ 62
           DE  +AMNLDLNLGPIPEPLSNS+PNE+ NLA LTEDPV RFGEAIRFRTRQRWRRRQVQ
Sbjct: 3   DEIFDAMNLDLNLGPIPEPLSNSVPNEDVNLADLTEDPVHRFGEAIRFRTRQRWRRRQVQ 62

Query: 63  IPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEKA 122
           IP ET+NI+M+LSEIMVNSGNGNSLQPLQAGEGS TAEERKNE GKTCEINNEALEDEK 
Sbjct: 63  IPSETRNITMELSEIMVNSGNGNSLQPLQAGEGSITAEERKNETGKTCEINNEALEDEKT 122

Query: 123 ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK 182
           ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK
Sbjct: 123 ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK 182

Query: 183 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIRR 242
           GEVTTKNVTPIYGRGS+TPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE+MIRR
Sbjct: 183 GEVTTKNVTPIYGRGSSTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEDMIRR 242

Query: 243 LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHAD 302
           LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRG+RREQISLP HDDVGDLAH D
Sbjct: 243 LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGIRREQISLPHHDDVGDLAHTD 302

Query: 303 VSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PPP 362
           V+ IEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGF+YTDPL+PR+QEQ PPP
Sbjct: 303 VASIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFLYTDPLIPRSQEQPPPP 362

Query: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 422
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRR+DALQVSDVDSGNSRAPRRR
Sbjct: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRSDALQVSDVDSGNSRAPRRR 422

Query: 423 RLA 425
           RLA
Sbjct: 423 RLA 425

BLAST of MS022987.1 vs. NCBI nr
Match: XP_008443034.1 (PREDICTED: uncharacterized protein LOC103486753 [Cucumis melo] >KAA0043743.1 RING finger protein 219 [Cucumis melo var. makuwa] >TYK25389.1 RING finger protein 219 [Cucumis melo var. makuwa])

HSP 1 Score: 769.2 bits (1985), Expect = 1.8e-218
Identity = 390/423 (92.20%), Postives = 405/423 (95.74%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQVQ 62
           DE  +AMNLDLNLGPIPEPLSNS+PNE+ NLA LTE+PV RFGE IRFRTRQRWRRRQVQ
Sbjct: 3   DEMFDAMNLDLNLGPIPEPLSNSVPNEDVNLADLTEEPVHRFGETIRFRTRQRWRRRQVQ 62

Query: 63  IPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEKA 122
           IPPETQNI+M+LSEIMVNSGNGNSLQPLQ GEGS TAEERKNE GKTCEINNEALEDEK 
Sbjct: 63  IPPETQNITMELSEIMVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALEDEKT 122

Query: 123 ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK 182
           ENKSDVEK SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHS+AKECPVCK
Sbjct: 123 ENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCK 182

Query: 183 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIRR 242
           GEVTTKNVTPIYGRGSNTP TEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE+MIRR
Sbjct: 183 GEVTTKNVTPIYGRGSNTPATEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEDMIRR 242

Query: 243 LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHAD 302
           LGNRFDFNRDLDQPPEPDSS+ETFGRSPTLLNRILTSRG+RREQISL PHDDVGDLAH D
Sbjct: 243 LGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRREQISL-PHDDVGDLAHTD 302

Query: 303 VSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PPP 362
           VS IE GETRLQSLPVLRSLLHRTRVSSL SAFNSADRLP+GF+YTDPL+PR+QEQ PPP
Sbjct: 303 VSSIETGETRLQSLPVLRSLLHRTRVSSLASAFNSADRLPEGFLYTDPLIPRSQEQPPPP 362

Query: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 422
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRRNDALQVSDVDSGNSRAPRRR
Sbjct: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAPRRR 422

Query: 423 RLA 425
           RLA
Sbjct: 423 RLA 424

BLAST of MS022987.1 vs. NCBI nr
Match: XP_022982955.1 (uncharacterized protein LOC111481648 [Cucurbita maxima])

HSP 1 Score: 766.9 bits (1979), Expect = 9.0e-218
Identity = 391/424 (92.22%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPN-EEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 62
           DE  +AMNLDLNLGPIPEPLSNS+   E+ NLA LTEDPV RFGEAIRFRTRQRWRRRQV
Sbjct: 3   DEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRRQV 62

Query: 63  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 122
           QIP ETQNI+M+LSEIMVNSGNGN+LQPLQAGEGS TAEERKNEAGKTCEINNEALEDEK
Sbjct: 63  QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALEDEK 122

Query: 123 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 182
            ENKSDVEK SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC
Sbjct: 123 TENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 182

Query: 183 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 242
           KGEVTTKNVTPIYGRG NTPVTEEDATLKIPLRPHARKVESLRQTIQRTA SFPVE+MIR
Sbjct: 183 KGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDMIR 242

Query: 243 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 302
           RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNR+LTSRG+RREQISLPPHDDVGDL+HA
Sbjct: 243 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRLLTSRGIRREQISLPPHDDVGDLSHA 302

Query: 303 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PP 362
           DVSGIE GETRLQSLPVLRSLLHRTRVSSLTSAFNS DRLPDG +YTDPL+PR QEQ PP
Sbjct: 303 DVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSTDRLPDGLLYTDPLIPRIQEQPPP 362

Query: 363 PVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRR 422
           PVEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRR DALQVSDVDSGNSRAPRR
Sbjct: 363 PVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAPRR 422

Query: 423 RRLA 425
           RRLA
Sbjct: 423 RRLA 426

BLAST of MS022987.1 vs. NCBI nr
Match: KAG6580801.1 (E3 ubiquitin-protein ligase RNF5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 762.7 bits (1968), Expect = 1.7e-216
Identity = 390/425 (91.76%), Postives = 404/425 (95.06%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPN-EEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 62
           DE  +AMNLDLNLGPIPEPLSNS+   E+ NLA LTEDPV RFGEAIRFRTRQRWRRRQV
Sbjct: 3   DEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRRQV 62

Query: 63  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 122
           QIP ETQNI+M+LSEIMVNSGNGN+LQPLQAGEGS TAEERKNEAGKTCEINNEALEDEK
Sbjct: 63  QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALEDEK 122

Query: 123 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 182
            ENKSDVEK SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHS+AKECPVC
Sbjct: 123 TENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVC 182

Query: 183 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 242
           KGEVTTKNVTPIYGRG NTPVTEEDATLKIPLRPHARKVESLRQTIQRTA SFPVE+MIR
Sbjct: 183 KGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDMIR 242

Query: 243 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 302
           RLGNRFDFNRD DQPPEPDSSHETFGRSPTLLNR+LTSRG+RREQISLPP+DDVGDLAHA
Sbjct: 243 RLGNRFDFNRDHDQPPEPDSSHETFGRSPTLLNRLLTSRGIRREQISLPPNDDVGDLAHA 302

Query: 303 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ--P 362
           DVSGIE GETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDG +YTDPL+PR QEQ  P
Sbjct: 303 DVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGLLYTDPLIPRIQEQQPP 362

Query: 363 PPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPR 422
           PPVEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRR DALQVSDVDSGNSRAPR
Sbjct: 363 PPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAPR 422

Query: 423 RRRLA 425
           RRRLA
Sbjct: 423 RRRLA 427

BLAST of MS022987.1 vs. ExPASy Swiss-Prot
Match: Q99942 (E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 3.9e-22
Identity = 44/84 (52.38%), Postives = 59/84 (70.24%), Query Frame = 0

Query: 136 GSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCKGEVTTKNVTPIYG 195
           G+ F+CNICL+ AR+ VV+ CGHLYCWPCL++WL    E +ECPVCK  ++ + V P+YG
Sbjct: 22  GATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYG 81

Query: 196 RGSNTPVTEEDATLKIPLRPHARK 220
           RGS  P   +D  LK P RP  ++
Sbjct: 82  RGSQKP---QDPRLKTPPRPQGQR 102

BLAST of MS022987.1 vs. ExPASy Swiss-Prot
Match: O35445 (E3 ubiquitin-protein ligase RNF5 OS=Mus musculus OX=10090 GN=Rnf5 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.3e-21
Identity = 42/81 (51.85%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 139 FDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCKGEVTTKNVTPIYGRGS 198
           F+CNICL+ AR+ VV+ CGHLYCWPCL++WL    + +ECPVCK  ++ + V P+YGRGS
Sbjct: 25  FECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISREKVVPLYGRGS 84

Query: 199 NTPVTEEDATLKIPLRPHARK 220
             P   +D  LK P RP  ++
Sbjct: 85  QKP---QDPRLKTPPRPQGQR 102

BLAST of MS022987.1 vs. ExPASy Swiss-Prot
Match: Q5M807 (E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus OX=10116 GN=Rnf5 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.3e-21
Identity = 42/81 (51.85%), Postives = 57/81 (70.37%), Query Frame = 0

Query: 139 FDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCKGEVTTKNVTPIYGRGS 198
           F+CNICL+ AR+ VV+ CGHLYCWPCL++WL    + +ECPVCK  ++ + V P+YGRGS
Sbjct: 25  FECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISREKVVPLYGRGS 84

Query: 199 NTPVTEEDATLKIPLRPHARK 220
             P   +D  LK P RP  ++
Sbjct: 85  QKP---QDPRLKTPPRPQGQR 102

BLAST of MS022987.1 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.6e-20
Identity = 44/93 (47.31%), Postives = 61/93 (65.59%), Query Frame = 0

Query: 131 SSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHS--------EAKECPVCK 190
           S D+D S FDCNICLD  ++PVVT CGHL+CWPC+++WL + S          ++CPVCK
Sbjct: 38  SDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCK 97

Query: 191 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRP 216
            +V+   + P+YGRG  T  T+E+    +P RP
Sbjct: 98  SKVSHSTLVPLYGRGRCT--TQEEGKNSVPKRP 128

BLAST of MS022987.1 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 6.2e-20
Identity = 44/95 (46.32%), Postives = 60/95 (63.16%), Query Frame = 0

Query: 134 SDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLH-------SEAKECPVCKGEVT 193
           ++   FDCNICLD A DPVVT CGHL+CWPC+Y+WLH+             CPVCK  +T
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 194 TKNVTPIYGRGSNTPVT-----EEDA-TLKIPLRP 216
             ++ P+YGRG ++P +     ++DA +  IP RP
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRP 131

BLAST of MS022987.1 vs. ExPASy TrEMBL
Match: A0A6J1DB37 (uncharacterized protein LOC111019352 OS=Momordica charantia OX=3673 GN=LOC111019352 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.8e-238
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 2   MDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 61
           MDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV
Sbjct: 1   MDESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 60

Query: 62  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 121
           QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK
Sbjct: 61  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 120

Query: 122 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 181
           AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC
Sbjct: 121 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 180

Query: 182 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 241
           KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR
Sbjct: 181 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 240

Query: 242 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 301
           RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA
Sbjct: 241 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 300

Query: 302 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPP 361
           DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPP
Sbjct: 301 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQPPP 360

Query: 362 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 421
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR
Sbjct: 361 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 420

Query: 422 RLA 425
           RLA
Sbjct: 421 RLA 423

BLAST of MS022987.1 vs. ExPASy TrEMBL
Match: A0A5A7TJV6 (RING finger protein 219 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005310 PE=4 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 8.8e-219
Identity = 390/423 (92.20%), Postives = 405/423 (95.74%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQVQ 62
           DE  +AMNLDLNLGPIPEPLSNS+PNE+ NLA LTE+PV RFGE IRFRTRQRWRRRQVQ
Sbjct: 3   DEMFDAMNLDLNLGPIPEPLSNSVPNEDVNLADLTEEPVHRFGETIRFRTRQRWRRRQVQ 62

Query: 63  IPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEKA 122
           IPPETQNI+M+LSEIMVNSGNGNSLQPLQ GEGS TAEERKNE GKTCEINNEALEDEK 
Sbjct: 63  IPPETQNITMELSEIMVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALEDEKT 122

Query: 123 ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK 182
           ENKSDVEK SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHS+AKECPVCK
Sbjct: 123 ENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCK 182

Query: 183 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIRR 242
           GEVTTKNVTPIYGRGSNTP TEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE+MIRR
Sbjct: 183 GEVTTKNVTPIYGRGSNTPATEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEDMIRR 242

Query: 243 LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHAD 302
           LGNRFDFNRDLDQPPEPDSS+ETFGRSPTLLNRILTSRG+RREQISL PHDDVGDLAH D
Sbjct: 243 LGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRREQISL-PHDDVGDLAHTD 302

Query: 303 VSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PPP 362
           VS IE GETRLQSLPVLRSLLHRTRVSSL SAFNSADRLP+GF+YTDPL+PR+QEQ PPP
Sbjct: 303 VSSIETGETRLQSLPVLRSLLHRTRVSSLASAFNSADRLPEGFLYTDPLIPRSQEQPPPP 362

Query: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 422
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRRNDALQVSDVDSGNSRAPRRR
Sbjct: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAPRRR 422

Query: 423 RLA 425
           RLA
Sbjct: 423 RLA 424

BLAST of MS022987.1 vs. ExPASy TrEMBL
Match: A0A1S3B7W0 (uncharacterized protein LOC103486753 OS=Cucumis melo OX=3656 GN=LOC103486753 PE=4 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 8.8e-219
Identity = 390/423 (92.20%), Postives = 405/423 (95.74%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQVQ 62
           DE  +AMNLDLNLGPIPEPLSNS+PNE+ NLA LTE+PV RFGE IRFRTRQRWRRRQVQ
Sbjct: 3   DEMFDAMNLDLNLGPIPEPLSNSVPNEDVNLADLTEEPVHRFGETIRFRTRQRWRRRQVQ 62

Query: 63  IPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEKA 122
           IPPETQNI+M+LSEIMVNSGNGNSLQPLQ GEGS TAEERKNE GKTCEINNEALEDEK 
Sbjct: 63  IPPETQNITMELSEIMVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALEDEKT 122

Query: 123 ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK 182
           ENKSDVEK SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHS+AKECPVCK
Sbjct: 123 ENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCK 182

Query: 183 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIRR 242
           GEVTTKNVTPIYGRGSNTP TEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE+MIRR
Sbjct: 183 GEVTTKNVTPIYGRGSNTPATEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEDMIRR 242

Query: 243 LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHAD 302
           LGNRFDFNRDLDQPPEPDSS+ETFGRSPTLLNRILTSRG+RREQISL PHDDVGDLAH D
Sbjct: 243 LGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRREQISL-PHDDVGDLAHTD 302

Query: 303 VSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PPP 362
           VS IE GETRLQSLPVLRSLLHRTRVSSL SAFNSADRLP+GF+YTDPL+PR+QEQ PPP
Sbjct: 303 VSSIETGETRLQSLPVLRSLLHRTRVSSLASAFNSADRLPEGFLYTDPLIPRSQEQPPPP 362

Query: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 422
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRRNDALQVSDVDSGNSRAPRRR
Sbjct: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAPRRR 422

Query: 423 RLA 425
           RLA
Sbjct: 423 RLA 424

BLAST of MS022987.1 vs. ExPASy TrEMBL
Match: A0A6J1J4B2 (uncharacterized protein LOC111481648 OS=Cucurbita maxima OX=3661 GN=LOC111481648 PE=4 SV=1)

HSP 1 Score: 766.9 bits (1979), Expect = 4.4e-218
Identity = 391/424 (92.22%), Postives = 404/424 (95.28%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPN-EEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQV 62
           DE  +AMNLDLNLGPIPEPLSNS+   E+ NLA LTEDPV RFGEAIRFRTRQRWRRRQV
Sbjct: 3   DEIFDAMNLDLNLGPIPEPLSNSVLQIEDVNLADLTEDPVHRFGEAIRFRTRQRWRRRQV 62

Query: 63  QIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEK 122
           QIP ETQNI+M+LSEIMVNSGNGN+LQPLQAGEGS TAEERKNEAGKTCEINNEALEDEK
Sbjct: 63  QIPSETQNITMELSEIMVNSGNGNTLQPLQAGEGSITAEERKNEAGKTCEINNEALEDEK 122

Query: 123 AENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 182
            ENKSDVEK SDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC
Sbjct: 123 TENKSDVEKGSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVC 182

Query: 183 KGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIR 242
           KGEVTTKNVTPIYGRG NTPVTEEDATLKIPLRPHARKVESLRQTIQRTA SFPVE+MIR
Sbjct: 183 KGEVTTKNVTPIYGRGGNTPVTEEDATLKIPLRPHARKVESLRQTIQRTALSFPVEDMIR 242

Query: 243 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHA 302
           RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNR+LTSRG+RREQISLPPHDDVGDL+HA
Sbjct: 243 RLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRLLTSRGIRREQISLPPHDDVGDLSHA 302

Query: 303 DVSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PP 362
           DVSGIE GETRLQSLPVLRSLLHRTRVSSLTSAFNS DRLPDG +YTDPL+PR QEQ PP
Sbjct: 303 DVSGIETGETRLQSLPVLRSLLHRTRVSSLTSAFNSTDRLPDGLLYTDPLIPRIQEQPPP 362

Query: 363 PVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRR 422
           PVEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRR DALQVSDVDSGNSRAPRR
Sbjct: 363 PVEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRTDALQVSDVDSGNSRAPRR 422

Query: 423 RRLA 425
           RRLA
Sbjct: 423 RRLA 426

BLAST of MS022987.1 vs. ExPASy TrEMBL
Match: A0A0A0LB96 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G806220 PE=4 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.1e-216
Identity = 387/423 (91.49%), Postives = 404/423 (95.51%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAIRFRTRQRWRRRQVQ 62
           DE  +AMNLDLNLGPIPEPLSNS+PNE+ NLA LTE+ V RFGE IRFRTRQRWRRRQVQ
Sbjct: 3   DEIFDAMNLDLNLGPIPEPLSNSVPNEDVNLADLTEESVHRFGETIRFRTRQRWRRRQVQ 62

Query: 63  IPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEALEDEKA 122
           IPPETQNI+M+LSEI+VNSGNGNSLQPLQ GEGS TAEERKNE GKTCEINNEALED K 
Sbjct: 63  IPPETQNITMELSEIIVNSGNGNSLQPLQPGEGSITAEERKNETGKTCEINNEALEDGKT 122

Query: 123 ENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKECPVCK 182
           ENKSDVEK SDSDGSFFDCNICLDL+RDPVVTCCGHLYCWPCLYRWLHLHS+AKECPVCK
Sbjct: 123 ENKSDVEKGSDSDGSFFDCNICLDLSRDPVVTCCGHLYCWPCLYRWLHLHSDAKECPVCK 182

Query: 183 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVEEMIRR 242
           GEVTTKNVTPIYGRGSNTPVTEEDATLKIP RPHARKVESLRQTIQRT FSFPVE+MIRR
Sbjct: 183 GEVTTKNVTPIYGRGSNTPVTEEDATLKIPPRPHARKVESLRQTIQRTPFSFPVEDMIRR 242

Query: 243 LGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQISLPPHDDVGDLAHAD 302
           LGNRFDFNRDLDQPPEPDSS+ETFGRSPTLLNRILTSRG+RRE+ISL PHDDVGDLAH D
Sbjct: 243 LGNRFDFNRDLDQPPEPDSSNETFGRSPTLLNRILTSRGIRRERISL-PHDDVGDLAHTD 302

Query: 303 VSGIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFIYTDPLVPRNQEQ-PPP 362
           VS IEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGF+YTDPL+PR+QEQ PPP
Sbjct: 303 VSSIEAGETRLQSLPVLRSLLHRTRVSSLTSAFNSADRLPDGFLYTDPLIPRSQEQPPPP 362

Query: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSSRRRNDALQVSDVDSGNSRAPRRR 422
           VEDRDSFSSIAAVINSESQMDTAVEIDSMVS+STSSSRRRNDALQVSDVDSGNSRAPRRR
Sbjct: 363 VEDRDSFSSIAAVINSESQMDTAVEIDSMVSISTSSSRRRNDALQVSDVDSGNSRAPRRR 422

Query: 423 RLA 425
           RLA
Sbjct: 423 RLA 424

BLAST of MS022987.1 vs. TAIR 10
Match: AT3G58030.1 (RING/U-box superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 9.9e-106
Identity = 237/447 (53.02%), Postives = 297/447 (66.44%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAI-RFRTRQRWRRRQV 62
           +E A+ MNLDLNLGP PE      PNE  NLA  T DP  R  EA+ R RTR R R RQ+
Sbjct: 3   EELADTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPERSSEAVTRIRTRHRTRFRQL 62

Query: 63  QIP----PETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEAL 122
            +P     ET  ++++L+++M NS N  +   +Q GEGS    ER NE  K CE  + AL
Sbjct: 63  NLPIPVLSETHTMAIELNQLMGNSVNRAA---MQTGEGS----ERGNEDLKMCENGDGAL 122

Query: 123 EDEKAENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKE 182
            D   + K+DVEKSS SDG+FFDCNICLDL+++PV+TCCGHLYCWPCLY+WL + S+AKE
Sbjct: 123 GDGVLDKKADVEKSSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKE 182

Query: 183 CPVCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE 242
           CPVCKGEVT+K VTPIYGRG++    EE    K+P+RPHAR++ESLR TIQR+ F+ P+E
Sbjct: 183 CPVCKGEVTSKTVTPIYGRGNHKREIEESLDTKVPMRPHARRIESLRNTIQRSPFTIPME 242

Query: 243 EMIRRLGNRFDFNRDLDQPPEPD-----SSHETFGRSPTLLNRILTSRGMRREQ------ 302
           EMIRR+ NRF    D D  P PD     +S     R+ ++LNR++TSRG+R EQ      
Sbjct: 243 EMIRRIQNRF----DRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASAA 302

Query: 303 ----ISLPPHDDVGDLAHADVSGIEAGETRLQSLPVLRSL-LHR-TRVSSLTSAFNSADR 362
               ++     D+      D+ G     TR   L + R L  HR  R+S+ TSA +SA+R
Sbjct: 303 AAAIVAASEDIDLNPNIAPDLEG--ESNTRFHPLLIRRQLQSHRVARISTFTSALSSAER 362

Query: 363 LPDGFIYTDPLVPRNQEQ----PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTS 422
           L D +  T PL   +QEQ    P  V+DRDSFSSIAAVINSESQ+DTAVEIDSM +LSTS
Sbjct: 363 LVDAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSESQVDTAVEIDSM-ALSTS 422

BLAST of MS022987.1 vs. TAIR 10
Match: AT3G58030.2 (RING/U-box superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 9.9e-106
Identity = 237/447 (53.02%), Postives = 297/447 (66.44%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAI-RFRTRQRWRRRQV 62
           +E A+ MNLDLNLGP PE      PNE  NLA  T DP  R  EA+ R RTR R R RQ+
Sbjct: 3   EELADTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPERSSEAVTRIRTRHRTRFRQL 62

Query: 63  QIP----PETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEAL 122
            +P     ET  ++++L+++M NS N  +   +Q GEGS    ER NE  K CE  + AL
Sbjct: 63  NLPIPVLSETHTMAIELNQLMGNSVNRAA---MQTGEGS----ERGNEDLKMCENGDGAL 122

Query: 123 EDEKAENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKE 182
            D   + K+DVEKSS SDG+FFDCNICLDL+++PV+TCCGHLYCWPCLY+WL + S+AKE
Sbjct: 123 GDGVLDKKADVEKSSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKE 182

Query: 183 CPVCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE 242
           CPVCKGEVT+K VTPIYGRG++    EE    K+P+RPHAR++ESLR TIQR+ F+ P+E
Sbjct: 183 CPVCKGEVTSKTVTPIYGRGNHKREIEESLDTKVPMRPHARRIESLRNTIQRSPFTIPME 242

Query: 243 EMIRRLGNRFDFNRDLDQPPEPD-----SSHETFGRSPTLLNRILTSRGMRREQ------ 302
           EMIRR+ NRF    D D  P PD     +S     R+ ++LNR++TSRG+R EQ      
Sbjct: 243 EMIRRIQNRF----DRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASAA 302

Query: 303 ----ISLPPHDDVGDLAHADVSGIEAGETRLQSLPVLRSL-LHR-TRVSSLTSAFNSADR 362
               ++     D+      D+ G     TR   L + R L  HR  R+S+ TSA +SA+R
Sbjct: 303 AAAIVAASEDIDLNPNIAPDLEG--ESNTRFHPLLIRRQLQSHRVARISTFTSALSSAER 362

Query: 363 LPDGFIYTDPLVPRNQEQ----PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTS 422
           L D +  T PL   +QEQ    P  V+DRDSFSSIAAVINSESQ+DTAVEIDSM +LSTS
Sbjct: 363 LVDAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSESQVDTAVEIDSM-ALSTS 422

BLAST of MS022987.1 vs. TAIR 10
Match: AT3G58030.3 (RING/U-box superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 9.9e-106
Identity = 237/447 (53.02%), Postives = 297/447 (66.44%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAI-RFRTRQRWRRRQV 62
           +E A+ MNLDLNLGP PE      PNE  NLA  T DP  R  EA+ R RTR R R RQ+
Sbjct: 3   EELADTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPERSSEAVTRIRTRHRTRFRQL 62

Query: 63  QIP----PETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEAL 122
            +P     ET  ++++L+++M NS N  +   +Q GEGS    ER NE  K CE  + AL
Sbjct: 63  NLPIPVLSETHTMAIELNQLMGNSVNRAA---MQTGEGS----ERGNEDLKMCENGDGAL 122

Query: 123 EDEKAENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKE 182
            D   + K+DVEKSS SDG+FFDCNICLDL+++PV+TCCGHLYCWPCLY+WL + S+AKE
Sbjct: 123 GDGVLDKKADVEKSSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKE 182

Query: 183 CPVCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE 242
           CPVCKGEVT+K VTPIYGRG++    EE    K+P+RPHAR++ESLR TIQR+ F+ P+E
Sbjct: 183 CPVCKGEVTSKTVTPIYGRGNHKREIEESLDTKVPMRPHARRIESLRNTIQRSPFTIPME 242

Query: 243 EMIRRLGNRFDFNRDLDQPPEPD-----SSHETFGRSPTLLNRILTSRGMRREQ------ 302
           EMIRR+ NRF    D D  P PD     +S     R+ ++LNR++TSRG+R EQ      
Sbjct: 243 EMIRRIQNRF----DRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASAA 302

Query: 303 ----ISLPPHDDVGDLAHADVSGIEAGETRLQSLPVLRSL-LHR-TRVSSLTSAFNSADR 362
               ++     D+      D+ G     TR   L + R L  HR  R+S+ TSA +SA+R
Sbjct: 303 AAAIVAASEDIDLNPNIAPDLEG--ESNTRFHPLLIRRQLQSHRVARISTFTSALSSAER 362

Query: 363 LPDGFIYTDPLVPRNQEQ----PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTS 422
           L D +  T PL   +QEQ    P  V+DRDSFSSIAAVINSESQ+DTAVEIDSM +LSTS
Sbjct: 363 LVDAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSESQVDTAVEIDSM-ALSTS 422

BLAST of MS022987.1 vs. TAIR 10
Match: AT3G58030.4 (RING/U-box superfamily protein )

HSP 1 Score: 381.3 bits (978), Expect = 9.9e-106
Identity = 237/447 (53.02%), Postives = 297/447 (66.44%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIPEPLSNSIPNEEENLAYLTEDPVRRFGEAI-RFRTRQRWRRRQV 62
           +E A+ MNLDLNLGP PE      PNE  NLA  T DP  R  EA+ R RTR R R RQ+
Sbjct: 3   EELADTMNLDLNLGPGPESDLQPAPNETVNLADWTNDPPERSSEAVTRIRTRHRTRFRQL 62

Query: 63  QIP----PETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEAL 122
            +P     ET  ++++L+++M NS N  +   +Q GEGS    ER NE  K CE  + AL
Sbjct: 63  NLPIPVLSETHTMAIELNQLMGNSVNRAA---MQTGEGS----ERGNEDLKMCENGDGAL 122

Query: 123 EDEKAENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEAKE 182
            D   + K+DVEKSS SDG+FFDCNICLDL+++PV+TCCGHLYCWPCLY+WL + S+AKE
Sbjct: 123 GDGVLDKKADVEKSSGSDGNFFDCNICLDLSKEPVLTCCGHLYCWPCLYQWLQI-SDAKE 182

Query: 183 CPVCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFPVE 242
           CPVCKGEVT+K VTPIYGRG++    EE    K+P+RPHAR++ESLR TIQR+ F+ P+E
Sbjct: 183 CPVCKGEVTSKTVTPIYGRGNHKREIEESLDTKVPMRPHARRIESLRNTIQRSPFTIPME 242

Query: 243 EMIRRLGNRFDFNRDLDQPPEPD-----SSHETFGRSPTLLNRILTSRGMRREQ------ 302
           EMIRR+ NRF    D D  P PD     +S     R+ ++LNR++TSRG+R EQ      
Sbjct: 243 EMIRRIQNRF----DRDSTPVPDFSNREASERVNDRANSILNRLMTSRGVRSEQNQASAA 302

Query: 303 ----ISLPPHDDVGDLAHADVSGIEAGETRLQSLPVLRSL-LHR-TRVSSLTSAFNSADR 362
               ++     D+      D+ G     TR   L + R L  HR  R+S+ TSA +SA+R
Sbjct: 303 AAAIVAASEDIDLNPNIAPDLEG--ESNTRFHPLLIRRQLQSHRVARISTFTSALSSAER 362

Query: 363 LPDGFIYTDPLVPRNQEQ----PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTS 422
           L D +  T PL   +QEQ    P  V+DRDSFSSIAAVINSESQ+DTAVEIDSM +LSTS
Sbjct: 363 LVDAYFRTHPLGRNHQEQNHHAPVVVDDRDSFSSIAAVINSESQVDTAVEIDSM-ALSTS 422

BLAST of MS022987.1 vs. TAIR 10
Match: AT2G42030.1 (RING/U-box superfamily protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-76
Identity = 205/445 (46.07%), Postives = 270/445 (60.67%), Query Frame = 0

Query: 3   DESAEAMNLDLNLGPIP----EPLSNSIPNEEEN-LAYLTEDPVRRFGEAIRFRTRQRWR 62
           +E +  +NLDLNLGP P    EP +N   ++  N LA    + +RRF    R R R R+R
Sbjct: 3   EEISSTVNLDLNLGPDPELGLEPATNYARSDWGNGLAARESESLRRF----RSRYRSRFR 62

Query: 63  R-RQVQIPPETQNISMQLSEIMVNSGNGNSLQPLQAGEGSTTAEERKNEAGKTCEINNEA 122
           R   + +  ET + +M+LS++++ SGN   +  L AGEGS  + ER  E  K CE  ++ 
Sbjct: 63  RIDMLPVLAETHSPAMELSQLLITSGN---VVALPAGEGSMASGERVIEDSKKCENGSKV 122

Query: 123 L-EDEKAENKSDVEKSSDSDGSFFDCNICLDLARDPVVTCCGHLYCWPCLYRWLHLHSEA 182
           + ED   E K DVEKS  SDGSFFDC ICLDL++DPVVT CGHLYCW CLY+WL + SEA
Sbjct: 123 MAEDNVTEEKRDVEKSVGSDGSFFDCYICLDLSKDPVVTNCGHLYCWSCLYQWLQV-SEA 182

Query: 183 KECPVCKGEVTTKNVTPIYGRGSNTPVTEEDATLKIPLRPHARKVESLRQTIQRTAFSFP 242
           KECPVCKGEV+ K VTPIYGRG     +EE +  KIP RP AR+ ESLR T+ R+    P
Sbjct: 183 KECPVCKGEVSVKTVTPIYGRGIQKRESEEVSNTKIPSRPQARRTESLRTTLNRSG-HIP 242

Query: 243 VEEMIRRLGNRFDFNRDLDQPPEPDSSHETFGRSPTLLNRILTSRGMRREQ------ISL 302
             E+ R L +R +            S+ E   R+   LNR +TSRG+R E+      +  
Sbjct: 243 T-EIFRHLQDRLE---------RESSTGER--RARPSLNRFMTSRGVRAEENQSSEALVE 302

Query: 303 PPHDDVGDLAHADVSGIEAGETRLQSLPVLRSLLHRT-------RVSSLTSAFNSADRLP 362
           P  D++ D+    ++     E   ++L   R+L  +        RVSS T   +SA+RL 
Sbjct: 303 PSSDEINDI-DLILNNAPENEEENENLSSSRALATQRRWAQMYGRVSSFT--LSSAERLA 362

Query: 363 DGFIYTDPLVPRNQEQ----PPPVEDRDSFSSIAAVINSESQMDTAVEIDSMVSLSTSSS 422
           D ++ T  L  RNQEQ    P  VEDRDSFSSI  VINSESQ++TA EI+SM+++STSSS
Sbjct: 363 DTYLITHAL-GRNQEQNSSPPVGVEDRDSFSSIVGVINSESQVETAAEINSMLTVSTSSS 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022151415.17.8e-238100.00uncharacterized protein LOC111019352 [Momordica charantia][more]
XP_038904860.11.0e-22193.38uncharacterized protein LOC120091094 isoform X1 [Benincasa hispida][more]
XP_008443034.11.8e-21892.20PREDICTED: uncharacterized protein LOC103486753 [Cucumis melo] >KAA0043743.1 RIN... [more]
XP_022982955.19.0e-21892.22uncharacterized protein LOC111481648 [Cucurbita maxima][more]
KAG6580801.11.7e-21691.76E3 ubiquitin-protein ligase RNF5, partial [Cucurbita argyrosperma subsp. sororia... [more]
Match NameE-valueIdentityDescription
Q999423.9e-2252.38E3 ubiquitin-protein ligase RNF5 OS=Homo sapiens OX=9606 GN=RNF5 PE=1 SV=1[more]
O354453.3e-2151.85E3 ubiquitin-protein ligase RNF5 OS=Mus musculus OX=10090 GN=Rnf5 PE=1 SV=1[more]
Q5M8073.3e-2151.85E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus OX=10116 GN=Rnf5 PE=2 SV=1[more]
O644253.6e-2047.31E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV... [more]
Q8GUK76.2e-2046.32E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DB373.8e-238100.00uncharacterized protein LOC111019352 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A5A7TJV68.8e-21992.20RING finger protein 219 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B7W08.8e-21992.20uncharacterized protein LOC103486753 OS=Cucumis melo OX=3656 GN=LOC103486753 PE=... [more]
A0A6J1J4B24.4e-21892.22uncharacterized protein LOC111481648 OS=Cucurbita maxima OX=3661 GN=LOC111481648... [more]
A0A0A0LB963.1e-21691.49RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G806220 P... [more]
Match NameE-valueIdentityDescription
AT3G58030.19.9e-10653.02RING/U-box superfamily protein [more]
AT3G58030.29.9e-10653.02RING/U-box superfamily protein [more]
AT3G58030.39.9e-10653.02RING/U-box superfamily protein [more]
AT3G58030.49.9e-10653.02RING/U-box superfamily protein [more]
AT2G42030.11.3e-7646.07RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 114..134
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 140..186
e-value: 1.1E-7
score: 31.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..135
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 393..424
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 250..269
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 393..413
NoneNo IPR availablePANTHERPTHR12313:SF71OS01G0830200 PROTEINcoord: 19..422
NoneNo IPR availableCDDcd16745RING-HC_AtRMA_likecoord: 139..183
e-value: 8.19332E-32
score: 113.168
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 137..194
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 141..181
e-value: 7.8E-8
score: 42.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 141..182
score: 13.226928
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 90..200
e-value: 1.1E-18
score: 69.0
IPR045103E3 ubiquitin-protein ligase RNF5/RNF185-likePANTHERPTHR12313E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATEDcoord: 19..422
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 156..165

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
MS022987MS022987gene


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
MS022987.1-cdsMS022987.1-cds-scaffold357:350008..351279CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
MS022987.1MS022987.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005783 endoplasmic reticulum
molecular_function GO:0061630 ubiquitin protein ligase activity