MS022452.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS022452.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Bet_v_1 domain-containing protein
Location	scaffold47: 3466633 .. 3467421 (+)
Sequence length	453
RNA-Seq Expression	MS022452.1
Synteny	MS022452.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAGCATGAAAGGAGAGGTGTTACTGAACCTGCCCGCAGAGAAAGCATGGCAGATGTACAGAGACAACGATGTGGTCAGTAAAATCAATCCTGAGCTGCTTTCTCGAGCTGAATATGTTCACGGAGATGGTGGTCCTGGTACTCTCAGGCTTTTCAAGCTTGGCCCAGGTATCTCTCTCTCTCTCACTCTAAAAATTCCTTGTTTCGATATCTTGTCCTGCCTACTTAGTACTTATAGAAACCTGTAATCTTCTTATTGTACCACTACCCCATGAGTCCATGACTAATGGGGCAGTTGTGGGCGAAAGGTAAGAGCCACAGTCTCAGTCTTTGCCACTGTACCTGAATTCTTAGTCTTTGTTGAACAATCCCTGAATGCAGAGAAGAGAAAAGAGACATTTTTCTCTGTTCTTTGTTGTCTCTCTCTTGTGCATGGGGAAGTATATTGTCCATTCCGTATTGGGTTAAAAAAAGGTGTTGTGATTATGATGATGACACGTGCAGCTGTGAGAGGCTACGTGGAAGAATCAGTGGAGAAGATAGAGAAGGTGGAGACAGGTAGATCAGTGAGCTACGACGTGGTGGGAGGAGAGCTGAGGAAGATGTACAATCCCTACAAAGTGACCTTCACATTCACTCCAGTGGAGGGAAAAGAGAAGGAAATGTGTGTTGCGCAGTGGAAAGCTGAATACGAGCCACTCACTCCCACAATTCCTCCGCCGGAGAAGGCAAGGGATGCTGCCTTGCAATTTCTCCAGTGCTTTGACAAGTTTCAGCTCAGCTAC

mRNA sequence

ATGAGGAGCATGAAAGGAGAGGTGTTACTGAACCTGCCCGCAGAGAAAGCATGGCAGATGTACAGAGACAACGATGTGGTCAGTAAAATCAATCCTGAGCTGCTTTCTCGAGCTGAATATGTTCACGGAGATGGTGGTCCTGGTACTCTCAGGCTTTTCAAGCTTGGCCCAGCTGTGAGAGGCTACGTGGAAGAATCAGTGGAGAAGATAGAGAAGGTGGAGACAGGTAGATCAGTGAGCTACGACGTGGTGGGAGGAGAGCTGAGGAAGATGTACAATCCCTACAAAGTGACCTTCACATTCACTCCAGTGGAGGGAAAAGAGAAGGAAATGTGTGTTGCGCAGTGGAAAGCTGAATACGAGCCACTCACTCCCACAATTCCTCCGCCGGAGAAGGCAAGGGATGCTGCCTTGCAATTTCTCCAGTGCTTTGACAAGTTTCAGCTCAGCTAC

Coding sequence (CDS)

ATGAGGAGCATGAAAGGAGAGGTGTTACTGAACCTGCCCGCAGAGAAAGCATGGCAGATGTACAGAGACAACGATGTGGTCAGTAAAATCAATCCTGAGCTGCTTTCTCGAGCTGAATATGTTCACGGAGATGGTGGTCCTGGTACTCTCAGGCTTTTCAAGCTTGGCCCAGCTGTGAGAGGCTACGTGGAAGAATCAGTGGAGAAGATAGAGAAGGTGGAGACAGGTAGATCAGTGAGCTACGACGTGGTGGGAGGAGAGCTGAGGAAGATGTACAATCCCTACAAAGTGACCTTCACATTCACTCCAGTGGAGGGAAAAGAGAAGGAAATGTGTGTTGCGCAGTGGAAAGCTGAATACGAGCCACTCACTCCCACAATTCCTCCGCCGGAGAAGGCAAGGGATGCTGCCTTGCAATTTCTCCAGTGCTTTGACAAGTTTCAGCTCAGCTAC

Protein sequence

MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVRGYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPLTPTIPPPEKARDAALQFLQCFDKFQLSY

Homology

[Close]

BLAST of MS022452.1 vs. NCBI nr
Match: XP_022147870.1 (uncharacterized protein LOC111016704 [Momordica charantia])

HSP 1 Score: 313.9 bits (803), Expect = 7.4e-82
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR
Sbjct: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
           GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY
Sbjct: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPLTPTIPPPEKARDAALQFLQCFDKFQLSY
Sbjct: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. NCBI nr
Match: XP_038887757.1 (major strawberry allergen Fra a 1.08-like [Benincasa hispida])

HSP 1 Score: 299.3 bits (765), Expect = 1.9e-77
Identity = 142/151 (94.04%), Postives = 147/151 (97.35%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           MRSMKGEVLL LPAEKAW+MYRDNDVVSKINPELLSRAEYV GDGGPGTLRLFKLGPAVR
Sbjct: 1   MRSMKGEVLLKLPAEKAWEMYRDNDVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVR 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
            YVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMC+AQW+AEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWEAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EP+TP IPPP+KARDAALQFLQCFDKFQLSY
Sbjct: 121 EPITPAIPPPDKARDAALQFLQCFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. NCBI nr
Match: XP_008455315.1 (PREDICTED: uncharacterized protein LOC103495511 [Cucumis melo])

HSP 1 Score: 296.2 bits (757), Expect = 1.6e-76
Identity = 142/151 (94.04%), Postives = 146/151 (96.69%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           M+SMKGEVLLNLPA+KAWQMYRDNDVVSKINPELLSRAEYV GDG PGTLRLFKLGPAV 
Sbjct: 1   MKSMKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGSPGTLRLFKLGPAVS 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
            YVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMC+AQWKAEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPLTPTIPPP+KARDAALQFLQ FDKFQLSY
Sbjct: 121 EPLTPTIPPPDKARDAALQFLQSFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. NCBI nr
Match: XP_004136826.1 (major strawberry allergen Fra a 1.08 [Cucumis sativus] >KGN43618.1 hypothetical protein Csa_020513 [Cucumis sativus])

HSP 1 Score: 293.5 bits (750), Expect = 1.0e-75
Identity = 141/151 (93.38%), Postives = 145/151 (96.03%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           M+SMKGEVLLNLPA+KAWQMYRDNDVVSKINPELLSRAEYV GDGGPGTLRLFKLGPAV 
Sbjct: 1   MKSMKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVS 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
            YVEESVEKIEKVETGRSVSYDVVGGELRKMY+PYKVTFTFTPVEGKEKEMC AQWKAEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYDPYKVTFTFTPVEGKEKEMCTAQWKAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPLTP IPPP+KARDAALQFLQ FDKFQLSY
Sbjct: 121 EPLTPAIPPPDKARDAALQFLQSFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. NCBI nr
Match: KAA0031572.1 (MLP-like protein 423 isoform X1 [Cucumis melo var. makuwa] >TYK07023.1 MLP-like protein 423 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 290.4 bits (742), Expect = 8.8e-75
Identity = 139/148 (93.92%), Postives = 142/148 (95.95%), Query Frame = 0


Query: 4   MKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVRGYV 63
           MKGEVLLNLPA+KAWQMYRDNDVVSKINPELLSRAEYV GDG PGTLRLFKLGPAV  YV
Sbjct: 1   MKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGSPGTLRLFKLGPAVSSYV 60

Query: 64  EESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPL 123
           EESVEKIEKVE GRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMC+AQWKAEYEPL
Sbjct: 61  EESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEYEPL 120

Query: 124 TPTIPPPEKARDAALQFLQCFDKFQLSY 152
           TPTIPPP+KARDAALQFLQ FDKFQLSY
Sbjct: 121 TPTIPPPDKARDAALQFLQSFDKFQLSY 148

[Close]

BLAST of MS022452.1 vs. ExPASy Swiss-Prot
Match: Q93VR4 (MLP-like protein 423 OS=Arabidopsis thaliana OX=3702 GN=MLP423 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 7   EVLLNLPAEKAWQMYRDN-DVVSKINPELLSRAEYVHGDG-GPGTLRLFKLGPAVRGYVE 66
           EV +  PAEK W    D  ++  K  P      + + GDG  PG++RL   G      V+
Sbjct: 10  EVEVKSPAEKFWVALGDGINLFPKAFPNDYKTIQVLAGDGNAPGSIRLITYGEG-SPLVK 69

Query: 67  ESVEKIEKVE-TGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPL 126
            S E+IE V+   +S+SY ++GGE+ + Y  +K T T  P +G      + +W  E+E  
Sbjct: 70  ISAERIEAVDLENKSMSYSIIGGEMLEYYKTFKGTITVIPKDGGS----LLKWSGEFEKT 129

Query: 127 TPTIPPPEKARDAALQFLQCFDKFQL 150
              I  P   +D A++  +  D++ L
Sbjct: 130 AHEIDDPHVIKDFAVKNFKEIDEYLL 150

[Close]

BLAST of MS022452.1 vs. ExPASy Swiss-Prot
Match: G7J032 (Phytohormone-binding protein OS=Medicago truncatula OX=3880 GN=MTR_3g055120 PE=1 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 2.6e-05
Identity = 41/156 (26.28%), Postives = 69/156 (44.23%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAW--QMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPA 60
           ++    +  LN+  E  W  Q      VV K+ P ++   + + GDGG GT  +F   P 
Sbjct: 2   IKEFNTQTTLNVGLEALWAAQSKDITLVVPKVLPNIVKDVQVIEGDGGVGTKLIFNFLPG 61

Query: 61  VR--GYVEESVEKIEKVETGRSVSYDVV-GGELRKMYNPYKVTFTFTPV-EGKEKEMCVA 120
           +    Y  E + + +  E   ++   VV GG L +  + YK TF F+ + E K       
Sbjct: 62  IAPVNYQREVITEYD--ELSHTIGLQVVEGGYLNQGLSYYKTTFQFSAISENKTLVNVKI 121

Query: 121 QWKAEYEPLTPTIPPPEKARDAALQFLQCFDKFQLS 151
            +  E E +   +  P K  ++ L +L   +KF L+
Sbjct: 122 SYDHESELIEEKV-KPTKTSESTLFYLGQLEKFLLN 154

[Close]

BLAST of MS022452.1 vs. ExPASy Swiss-Prot
Match: P27538 (Pathogenesis-related protein 2 OS=Petroselinum crispum OX=4043 GN=PR2 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 2.6e-05
Identity = 35/138 (25.36%), Postives = 67/138 (48.55%), Query Frame = 0


Query: 7   EVLLNLPAE---KAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVRGYV 66
           EV  ++PA+   K + +  DN ++ K+ P+ +   E + GDGG GT++   LG   +  V
Sbjct: 9   EVASSVPAQTIYKGFLLDMDN-IIPKVLPQAIKSIEIISGDGGAGTIKKVTLGEVSQFTV 68

Query: 67  EESVEKIEKVET-GRSVSYDVVGGE-LRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYE 126
            +  ++I++++      SY ++ G+ L  +       FT  P +G     C+ +    Y 
Sbjct: 69  VK--QRIDEIDAEALKYSYSIIEGDLLLGIIESITSKFTVVPTDGG----CIVKNTTIYT 128

Query: 127 PLTPTIPPPEKARDAALQ 140
           P+   + P E  ++A  Q
Sbjct: 129 PIGDAVIPEENVKEATEQ 139

[Close]

BLAST of MS022452.1 vs. ExPASy Swiss-Prot
Match: Q256S2 (Major strawberry allergen Fra a 1-E OS=Fragaria ananassa OX=3747 GN=FRAA1E.1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 4.5e-05
Identity = 26/86 (30.23%), Postives = 48/86 (55.81%), Query Frame = 0


Query: 5  KGEVLLNLPAEKAWQMY--RDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR-G 64
          + E   ++PA K ++ +    ++++ KI P+ +  AE + GDGGPGT++    G     G
Sbjct: 7  ENEFTSDIPAPKLFKAFVLDADNLIPKIAPQAVKCAEILEGDGGPGTIKKITFGEGSHYG 66

Query: 65 YVEESVEKIEKVETGRSVSYDVVGGE 88
          YV+  +  I+KV    + SY ++ G+
Sbjct: 67 YVKHKIHSIDKV--NHTYSYSLIEGD 90

[Close]

BLAST of MS022452.1 vs. ExPASy Swiss-Prot
Match: P92918 (Major allergen Api g 1, isoallergen 2 OS=Apium graveolens OX=4045 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 5.8e-05
Identity = 37/127 (29.13%), Postives = 62/127 (48.82%), Query Frame = 0


Query: 14  AEKAWQMY-RDNDVV-SKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR-GYVEESVEKI 73
           AEK +Q +  D D V  K+ P+L+   E + GDGG GT++L  LG A     +++ V+ I
Sbjct: 16  AEKMYQGFLLDMDTVFPKVLPQLIKSVEILEGDGGVGTVKLVHLGEATEYTTMKQKVDVI 75

Query: 74  EKVETGRSVSYDVVGGE-LRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPLTPTIPP 133
           +K   G + +Y  +GG+ L  +       F   P +G     C+ +    Y      + P
Sbjct: 76  DK--AGLAYTYTTIGGDILVDVLESVVNEFVVVPTDGG----CIVKNTTIYNTKGDAVLP 135

Query: 134 PEKARDA 137
            +K ++A
Sbjct: 136 EDKIKEA 136

[Close]

BLAST of MS022452.1 vs. ExPASy TrEMBL
Match: A0A6J1D1C2 (uncharacterized protein LOC111016704 OS=Momordica charantia OX=3673 GN=LOC111016704 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.6e-82
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR
Sbjct: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
           GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY
Sbjct: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPLTPTIPPPEKARDAALQFLQCFDKFQLSY
Sbjct: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. ExPASy TrEMBL
Match: A0A1S3C1C9 (uncharacterized protein LOC103495511 OS=Cucumis melo OX=3656 GN=LOC103495511 PE=3 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 7.7e-77
Identity = 142/151 (94.04%), Postives = 146/151 (96.69%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           M+SMKGEVLLNLPA+KAWQMYRDNDVVSKINPELLSRAEYV GDG PGTLRLFKLGPAV 
Sbjct: 1   MKSMKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGSPGTLRLFKLGPAVS 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
            YVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMC+AQWKAEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPLTPTIPPP+KARDAALQFLQ FDKFQLSY
Sbjct: 121 EPLTPTIPPPDKARDAALQFLQSFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. ExPASy TrEMBL
Match: A0A0A0K2C4 (Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G048020 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 5.0e-76
Identity = 141/151 (93.38%), Postives = 145/151 (96.03%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           M+SMKGEVLLNLPA+KAWQMYRDNDVVSKINPELLSRAEYV GDGGPGTLRLFKLGPAV 
Sbjct: 1   MKSMKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGGPGTLRLFKLGPAVS 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
            YVEESVEKIEKVETGRSVSYDVVGGELRKMY+PYKVTFTFTPVEGKEKEMC AQWKAEY
Sbjct: 61  SYVEESVEKIEKVETGRSVSYDVVGGELRKMYDPYKVTFTFTPVEGKEKEMCTAQWKAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPLTP IPPP+KARDAALQFLQ FDKFQLSY
Sbjct: 121 EPLTPAIPPPDKARDAALQFLQSFDKFQLSY 151

[Close]

BLAST of MS022452.1 vs. ExPASy TrEMBL
Match: A0A5A7SKH6 (MLP-like protein 423 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003390 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.3e-75
Identity = 139/148 (93.92%), Postives = 142/148 (95.95%), Query Frame = 0


Query: 4   MKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVRGYV 63
           MKGEVLLNLPA+KAWQMYRDNDVVSKINPELLSRAEYV GDG PGTLRLFKLGPAV  YV
Sbjct: 1   MKGEVLLNLPAQKAWQMYRDNDVVSKINPELLSRAEYVQGDGSPGTLRLFKLGPAVSSYV 60

Query: 64  EESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPL 123
           EESVEKIEKVE GRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMC+AQWKAEYEPL
Sbjct: 61  EESVEKIEKVEAGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCIAQWKAEYEPL 120

Query: 124 TPTIPPPEKARDAALQFLQCFDKFQLSY 152
           TPTIPPP+KARDAALQFLQ FDKFQLSY
Sbjct: 121 TPTIPPPDKARDAALQFLQSFDKFQLSY 148

[Close]

BLAST of MS022452.1 vs. ExPASy TrEMBL
Match: A0A6J1HZI6 (uncharacterized protein LOC111468337 OS=Cucurbita maxima OX=3661 GN=LOC111468337 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.2e-74
Identity = 137/151 (90.73%), Postives = 144/151 (95.36%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           MRSMKGEV LNLPAEKAWQMYRDN+V+SKINPELLSRAEYV GDGGPGTLRLFKLGPA+R
Sbjct: 1   MRSMKGEVSLNLPAEKAWQMYRDNEVISKINPELLSRAEYVQGDGGPGTLRLFKLGPALR 60

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
            YV ESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKE EMC AQW+AEY
Sbjct: 61  SYVGESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKETEMCTAQWQAEY 120

Query: 121 EPLTPTIPPPEKARDAALQFLQCFDKFQLSY 152
           EPL+PTIPPP+KARDAAL+FLQCFDKF LSY
Sbjct: 121 EPLSPTIPPPDKARDAALRFLQCFDKFDLSY 151

[Close]

BLAST of MS022452.1 vs. TAIR 10
Match: AT1G24020.1 (MLP-like protein 423 )

HSP 1 Score: 67.0 bits (162), Expect = 1.5e-11
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 7   EVLLNLPAEKAWQMYRDN-DVVSKINPELLSRAEYVHGDG-GPGTLRLFKLGPAVRGYVE 66
           EV +  PAEK W    D  ++  K  P      + + GDG  PG++RL   G      V+
Sbjct: 10  EVEVKSPAEKFWVALGDGINLFPKAFPNDYKTIQVLAGDGNAPGSIRLITYGEG-SPLVK 69

Query: 67  ESVEKIEKVE-TGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPL 126
            S E+IE V+   +S+SY ++GGE+ + Y  +K T T  P +G      + +W  E+E  
Sbjct: 70  ISAERIEAVDLENKSMSYSIIGGEMLEYYKTFKGTITVIPKDGGS----LLKWSGEFEKT 129

Query: 127 TPTIPPPEKARDAALQFLQCFDKFQL 150
              I  P   +D A++  +  D++ L
Sbjct: 130 AHEIDDPHVIKDFAVKNFKEIDEYLL 150

[Close]

BLAST of MS022452.1 vs. TAIR 10
Match: AT1G24020.2 (MLP-like protein 423 )

HSP 1 Score: 67.0 bits (162), Expect = 1.5e-11
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 7   EVLLNLPAEKAWQMYRDN-DVVSKINPELLSRAEYVHGDG-GPGTLRLFKLGPAVRGYVE 66
           EV +  PAEK W    D  ++  K  P      + + GDG  PG++RL   G      V+
Sbjct: 10  EVEVKSPAEKFWVALGDGINLFPKAFPNDYKTIQVLAGDGNAPGSIRLITYGEG-SPLVK 69

Query: 67  ESVEKIEKVE-TGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEYEPL 126
            S E+IE V+   +S+SY ++GGE+ + Y  +K T T  P +G      + +W  E+E  
Sbjct: 70  ISAERIEAVDLENKSMSYSIIGGEMLEYYKTFKGTITVIPKDGGS----LLKWSGEFEKT 129

Query: 127 TPTIPPPEKARDAALQFLQCFDKFQL 150
              I  P   +D A++  +  D++ L
Sbjct: 130 AHEIDDPHVIKDFAVKNFKEIDEYLL 150

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BLAST of MS022452.1 vs. TAIR 10
Match: AT1G23120.1 (Polyketide cyclase/dehydrase and lipid transport superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 1.9e-11
Identity = 38/132 (28.79%), Postives = 70/132 (53.03%), Query Frame = 0


Query: 1   MRSMKGEVLLNLPAEKAWQMYRDNDVVSKINPELLSRAEYVHGDGGPGTLRLFKLGPAVR 60
           +++++ E+ +N+ AE+ ++ ++  +     N    + A YVH D       +      V 
Sbjct: 4   VQTLELEIQVNMTAERFFKTFKKKEG----NFTDKTEAVYVHRDDPTSNSSIQIWNFIVD 63

Query: 61  GYVEESVEKIEKVETGRSVSYDVVGGELRKMYNPYKVTFTFTPVEGKEKEMCVAQWKAEY 120
           G +E+  EKI+  E  +SVS+  + G++ K Y  YK+T    P   K+ ++C+A+W  EY
Sbjct: 64  GKMEQIKEKIDVDEENKSVSFLALEGDVLKQYKSYKITLDVVP---KDHKVCIAKWTWEY 123

Query: 121 EPLTPTIPPPEK 133
           E L   +PPP +
Sbjct: 124 EKLNDDVPPPTR 128

The following BLAST results are available for this feature:

BLAST of MS022452.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147870.1	7.4e-82	100.00	uncharacterized protein LOC111016704 [Momordica charantia]	[more]
XP_038887757.1	1.9e-77	94.04	major strawberry allergen Fra a 1.08-like [Benincasa hispida]	[more]
XP_008455315.1	1.6e-76	94.04	PREDICTED: uncharacterized protein LOC103495511 [Cucumis melo]	[more]
XP_004136826.1	1.0e-75	93.38	major strawberry allergen Fra a 1.08 [Cucumis sativus] >KGN43618.1 hypothetical ...	[more]
KAA0031572.1	8.8e-75	93.92	MLP-like protein 423 isoform X1 [Cucumis melo var. makuwa] >TYK07023.1 MLP-like ...	[more]

BLAST of MS022452.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VR4	2.1e-10	30.82	MLP-like protein 423 OS=Arabidopsis thaliana OX=3702 GN=MLP423 PE=2 SV=1	[more]
G7J032	2.6e-05	26.28	Phytohormone-binding protein OS=Medicago truncatula OX=3880 GN=MTR_3g055120 PE=1...	[more]
P27538	2.6e-05	25.36	Pathogenesis-related protein 2 OS=Petroselinum crispum OX=4043 GN=PR2 PE=2 SV=1	[more]
Q256S2	4.5e-05	30.23	Major strawberry allergen Fra a 1-E OS=Fragaria ananassa OX=3747 GN=FRAA1E.1 PE=...	[more]
P92918	5.8e-05	29.13	Major allergen Api g 1, isoallergen 2 OS=Apium graveolens OX=4045 PE=1 SV=1	[more]

BLAST of MS022452.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1C2	3.6e-82	100.00	uncharacterized protein LOC111016704 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]
A0A1S3C1C9	7.7e-77	94.04	uncharacterized protein LOC103495511 OS=Cucumis melo OX=3656 GN=LOC103495511 PE=...	[more]
A0A0A0K2C4	5.0e-76	93.38	Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G048020 PE=...	[more]
A0A5A7SKH6	4.3e-75	93.92	MLP-like protein 423 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1HZI6	1.2e-74	90.73	uncharacterized protein LOC111468337 OS=Cucurbita maxima OX=3661 GN=LOC111468337...	[more]

BLAST of MS022452.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24020.1	1.5e-11	30.82	MLP-like protein 423	[more]
AT1G24020.2	1.5e-11	30.82	MLP-like protein 423	[more]
AT1G23120.1	1.9e-11	28.79	Polyketide cyclase/dehydrase and lipid transport superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000916	Bet v I/Major latex protein	SMART	SM01037	Bet_v_1_2	coord: 3..151 e-value: 1.5E-8 score: 44.4
IPR000916	Bet v I/Major latex protein	PFAM	PF00407	Bet_v_1	coord: 4..140 e-value: 3.7E-15 score: 56.1
IPR023393	START-like domain superfamily	GENE3D	3.30.530.20		coord: 1..150 e-value: 1.0E-27 score: 99.0
None	No IPR available	PANTHER	PTHR31213:SF97	OS08G0374000 PROTEIN	coord: 3..148
None	No IPR available	PANTHER	PTHR31213	FAMILY NOT NAMED	coord: 3..148
None	No IPR available	CDD	cd07816	Bet_v1-like	coord: 3..137 e-value: 3.96894E-33 score: 112.283
None	No IPR available	SUPERFAMILY	55961	Bet v1-like	coord: 1..148

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS022452	MS022452	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS022452.1-cds	MS022452.1-cds-scaffold47:3466633..3466804	CDS
MS022452.1-cds	MS022452.1-cds-scaffold47:3467141..3467421	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS022452.1	MS022452.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response