MS022358.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS022358.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	glucosidase 2 subunit beta
Location	scaffold47: 2771367 .. 2773397 (+)
Sequence length	603
RNA-Seq Expression	MS022358.1
Synteny	MS022358.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCGCGTCGTCCTCCAAAGTCCACGAGCTTCGTTTCCATGATTTCCATCTGTTTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTTATTGGAGTTCACCCCCTCGGTATTCCTCTCTCTCTTTGACCCTCTGCTTAATGTTGGCCCTGGATTAGAGGATTTGACACTGCGAAGCTCGAAAATGGAATTTATATCGCCATTTCTGCCCCTTCCTAGCTAACTGATTCCTTTTGGATTGTACCCAGTTTGTCAATTCATGTTCGTTCGGTGAACCTTTTTTCTGAATTGAAATGTAAATTTGATTGCAGACGAGAAGTATTATTCATCGGAGGTCATAAAGTGCAAAGACGGGTCTAGATCTTTCACCATTGACCGTCTGAACGACGATTTCTGCGACTGCGTCGATGGAACTGATGAGCCTGGTGCTTTCATTGTCCCTTCTTGAGTTTTAATCATCAAGGATTTTTAATGCATAATTTGATTTGAGCTGTTAATATCAATTTTTTTGTGTTTATTACATCAGCTTCTCAAATACCGTAGTGGTGAACTGATTGAGAGAAGTTTAGGGTTCTTTTGAATGAAATTGGAGGAACGGAGAAGATAATTTCCTGCTGCAAACGTCAATGTGAAGTGACTATTGAAATTACATTTTGAACGAAGGCAACAATTATTAGCCATTGCAATCTGTCTTACATGGGAACATGGAAAAATTTAATGTTCTATCGTGTTAACTATTAAAAAATACCTACCATGTTTAACCCAAAAGAGCATGAGTTGTTTTTCTTTCTGAATTCTCACCGACATGTTTAAAACTGGAATGTAGAAACCATATCTTCTTTGTCTCGTTGTTGTATCATTAGAAGGTAAGATAAGTTCTGCATAAGAATGATATTGGACTTTTAAGCAATAAATTCTAATTCTGGTATGGGCATTTGGTCATGTAAAAAGAAAGAAAAACAACTTCTCCCCATAGCCAAGAACAGCCCACACACAACACAGTGTGAGGATCAGGTGTGTTTTTTTTTCTGTGCTCTGTGCTGCTGGCTCGTGGAGACATTTGACCTTTGATCTTTGTATTTGTATATGCAGGTACTTCTGCCTGTGCAAGAGGGAAATTTTATTGTAGAAATATGGGAAGTACCCCACAGTTCATATTTTCTTCCCACGTCAATGATCGTATTTGTGGTAACTATCCTTTTAATCTTTTGTTTTCCTTCTTTTTCTATGCACATGGCTTGATTGGGAGTTTAATGACTTCCGGAAGTTTGGTCGAGGAAAGAAAGATGTGAATTGAAACACCATAAATCTGTTGATAAACATGTAGTACAACATTCCTGTGATGGTGTGGGTGATGCAATTAGTACACTTTGTACAAGCAGATGAGTTAATTGTGCTGATTATTTAGTCTTACTGCTTTTTTTTTTCTTTCCTGTCAATATAGGGTCTCTATTCTGTTTAAAGTCTGCAAATGGTAGTTAACACTTGACAATCGACATACTCCATAATTCTTGGAATAATCCTTACAGACCATTTTGTGTTGTGTTCAAAGATCCGTCACTTTTTCCACTCGTGTCGAATCGACAGTTGATGGGAGAAACTTGGGGTTTACATTTCATCTTCCAAATTTGAATTTGGATTCCAATTCCCTGTTCAACATAATTGCAGATTGCTGTGATGGAAGTGATGAATATGATGGTAATACCTTTTGTCCAAACACCTGTGTCATGGGGGGAAATATAGCGTACAAGAGCAACAATTATATTTCGACTACTCGAGATGTGCATATAGTCAACAGAAAAGTAAAGGAGGAAATCACCAAGGAGGACTTGTTTCAGAAGCTAATGGGTATGATTTTTGTTTCACGACTTACCAAATCGATTTCGTAGCACTCAATTAACTGTTGTGTTGTAACTAATCATTTCAGGTTTGAAGTTAGTAATAGTCCTACAGGTAGCTCTCACAAGTTTTGCAGTGTTCATATGGGTAAACCGTTGTCGTGCAAGATCTAAAAGAAGACGACACCGT

mRNA sequence

ATGGAGGCGCGTCGTCCTCCAAAGTCCACGAGCTTCGTTTCCATGATTTCCATCTGTTTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTTATTGGAGTTCACCCCCTCGACGAGAAGTATTATTCATCGGAGGTCATAAAGTGCAAAGACGGGTCTAGATCTTTCACCATTGACCGTCTGAACGACGATTTCTGCGACTGCGTCGATGGAACTGATGAGCCTGGTACTTCTGCCTGTGCAAGAGGGAAATTTTATTGTAGAAATATGGGAAGTACCCCACAGTTCATATTTTCTTCCCACGTCAATGATCGTATTTGTGATTGCTGTGATGGAAGTGATGAATATGATGGTAATACCTTTTGTCCAAACACCTGTGTCATGGGGGGAAATATAGCGTACAAGAGCAACAATTATATTTCGACTACTCGAGATGTGCATATAGTCAACAGAAAAGTAAAGGAGGAAATCACCAAGGAGGACTTGTTTCAGAAGCTAATGGGTTTGAAGTTAGTAATAGTCCTACAGGTAGCTCTCACAAGTTTTGCAGTGTTCATATGGGTAAACCGTTGTCGTGCAAGATCTAAAAGAAGACGACACCGT

Coding sequence (CDS)

ATGGAGGCGCGTCGTCCTCCAAAGTCCACGAGCTTCGTTTCCATGATTTCCATCTGTTTCTTCTTCGCTTCTGTTCGCTCTGTTCCATCACTTATTGGAGTTCACCCCCTCGACGAGAAGTATTATTCATCGGAGGTCATAAAGTGCAAAGACGGGTCTAGATCTTTCACCATTGACCGTCTGAACGACGATTTCTGCGACTGCGTCGATGGAACTGATGAGCCTGGTACTTCTGCCTGTGCAAGAGGGAAATTTTATTGTAGAAATATGGGAAGTACCCCACAGTTCATATTTTCTTCCCACGTCAATGATCGTATTTGTGATTGCTGTGATGGAAGTGATGAATATGATGGTAATACCTTTTGTCCAAACACCTGTGTCATGGGGGGAAATATAGCGTACAAGAGCAACAATTATATTTCGACTACTCGAGATGTGCATATAGTCAACAGAAAAGTAAAGGAGGAAATCACCAAGGAGGACTTGTTTCAGAAGCTAATGGGTTTGAAGTTAGTAATAGTCCTACAGGTAGCTCTCACAAGTTTTGCAGTGTTCATATGGGTAAACCGTTGTCGTGCAAGATCTAAAAGAAGACGACACCGT

Protein sequence

MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNTFCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALTSFAVFIWVNRCRARSKRRRHR

Homology

[Close]

BLAST of MS022358.1 vs. NCBI nr
Match: XP_022136263.1 (glucosidase 2 subunit beta [Momordica charantia])

HSP 1 Score: 408.3 bits (1048), Expect = 3.8e-110
Identity = 198/201 (98.51%), Postives = 199/201 (99.00%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA RPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEAGRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGNIAYKSNNYISTT+DVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT
Sbjct: 121 FCPNTCVMGGNIAYKSNNYISTTQDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
           SFAVFIWVNRCRARSKRRRHR
Sbjct: 181 SFAVFIWVNRCRARSKRRRHR 201

[Close]

BLAST of MS022358.1 vs. NCBI nr
Match: XP_038887466.1 (glucosidase 2 subunit beta [Benincasa hispida])

HSP 1 Score: 364.8 bits (935), Expect = 4.9e-97
Identity = 176/202 (87.13%), Postives = 187/202 (92.57%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           ME  R PK TSFVS++SICFFF SVRSVPSLIGVHPLDEKYY SEVIKCKDGS+SFTIDR
Sbjct: 1   METPRSPKPTSFVSLLSICFFFDSVRSVPSLIGVHPLDEKYYYSEVIKCKDGSKSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VNDRICDCCDGSDEYDGN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDRICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVH-IVNRKVKEEITKEDLFQKLMGLKLVIVLQVAL 180
           FCPNTCVMGGNI YKSNN ISTTRDV  IVNRKV+EEIT+ED+FQKL+GLKLVI+LQVAL
Sbjct: 121 FCPNTCVMGGNIVYKSNNDISTTRDVDIIVNRKVQEEITEEDIFQKLIGLKLVIILQVAL 180

Query: 181 TSFAVFIWVNRCRARSKRRRHR 202
           TSFA+ IW NRCR +SKRRRHR
Sbjct: 181 TSFAILIWANRCRVKSKRRRHR 202

[Close]

BLAST of MS022358.1 vs. NCBI nr
Match: XP_022952390.1 (glucosidase 2 subunit beta [Cucurbita moschata])

HSP 1 Score: 363.6 bits (932), Expect = 1.1e-96
Identity = 172/201 (85.57%), Postives = 185/201 (92.04%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA   PKS SFV ++S CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+F+FSS VND ICDCCDGSDEYDGN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN AYK++ YIST+RDV ++NRKVKEEITKEDLF +L+GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
            FAV IW NRCRA+SKRRRHR
Sbjct: 181 CFAVLIWANRCRAKSKRRRHR 201

[Close]

BLAST of MS022358.1 vs. NCBI nr
Match: XP_023554618.1 (glucosidase 2 subunit beta [Cucurbita pepo subsp. pepo])

HSP 1 Score: 362.1 bits (928), Expect = 3.2e-96
Identity = 171/201 (85.07%), Postives = 185/201 (92.04%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA   PKS SFV ++S CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+F+FSS VND ICDCCDGSDEYDGN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN AYK++ YIST+RDV ++NRKVKEEITKEDLF +L+GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
            FAV IW NRC+A+SKRRRHR
Sbjct: 181 CFAVLIWANRCQAKSKRRRHR 201

[Close]

BLAST of MS022358.1 vs. NCBI nr
Match: XP_022969237.1 (glucosidase 2 subunit beta [Cucurbita maxima])

HSP 1 Score: 354.0 bits (907), Expect = 8.6e-94
Identity = 168/199 (84.42%), Postives = 181/199 (90.95%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA    KS SFV ++S CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 39  MEAGHSTKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 98

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+F+FSS VND ICDCCDGSDEYDGN 
Sbjct: 99  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 158

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN AYK++ YIST+RDV ++NRKVKEEITKEDLF +L+GLKLVI+LQVAL 
Sbjct: 159 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALI 218

Query: 181 SFAVFIWVNRCRARSKRRR 200
           SFAV IW NRCR +SKRRR
Sbjct: 219 SFAVIIWANRCRVKSKRRR 237

[Close]

BLAST of MS022358.1 vs. ExPASy Swiss-Prot
Match: Q9FM96 (Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 1.2e-37
Identity = 89/171 (52.05%), Postives = 107/171 (62.57%), Query Frame = 0


Query: 10  TSFVSM---ISICFFFAS-VRSVPS-----LIGVHPLDEKYY-SSEVIKCKDGSRSFTID 69
           +SFVS+   +S     AS +RS  S      +G+ P DEKYY SS  IKCKDGS+ FT  
Sbjct: 7   SSFVSVSLQLSFLLLLASAIRSSSSPPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKA 66

Query: 70  RLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGN 129
           +LNDDFCDC DGTDEPGTSAC  GKFYCRN G +P  +FSS VND ICDCCDGSDEYDG+
Sbjct: 67  QLNDDFCDCSDGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGH 126

Query: 130 TFCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLK 171
             C NTC   G  A ++      T +  +V R+ + E  K  L +    LK
Sbjct: 127 VSCQNTCWEAGKAARENLKKKIETYNQGLVIRRQEIEQAKVGLEKDAAELK 177

[Close]

BLAST of MS022358.1 vs. ExPASy Swiss-Prot
Match: A2WNF5 (Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-35
Identity = 68/111 (61.26%), Postives = 77/111 (69.37%), Query Frame = 0


Query: 23  ASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACAR 82
           A+ R     +G+ P DE Y+   VI+C+DGS  F  D+LNDDFCDC DGTDEPGTSAC  
Sbjct: 19  AASRPPLDTLGIPPQDEAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDEPGTSACPE 78

Query: 83  GKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNTFCPNTCVMGGNIA 134
           GKFYC+N G +P  IFSS VND ICDCCDGSDEYD N  C NTC   G  A
Sbjct: 79  GKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAA 129

[Close]

BLAST of MS022358.1 vs. ExPASy Swiss-Prot
Match: Q5NBP9 (Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0276800 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-35
Identity = 68/111 (61.26%), Postives = 77/111 (69.37%), Query Frame = 0


Query: 23  ASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACAR 82
           A+ R     +G+ P DE Y+   VI+C+DGS  F  D+LNDDFCDC DGTDEPGTSAC  
Sbjct: 19  AASRPPLDTLGIPPQDEAYFRGGVIRCRDGSGRFARDKLNDDFCDCPDGTDEPGTSACPE 78

Query: 83  GKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNTFCPNTCVMGGNIA 134
           GKFYC+N G +P  IFSS VND ICDCCDGSDEYD N  C NTC   G  A
Sbjct: 79  GKFYCQNAGHSPITIFSSRVNDGICDCCDGSDEYDSNVTCKNTCWEAGKAA 129

[Close]

BLAST of MS022358.1 vs. ExPASy Swiss-Prot
Match: O08795 (Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.3e-25
Identity = 57/133 (42.86%), Postives = 81/133 (60.90%), Query Frame = 0


Query: 41  YYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSS 100
           Y  S+   C DG+ +   D++NDD+CDC DG+DEPGT+AC  G F+C N G  P +I SS
Sbjct: 31  YEESKPFTCLDGTATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYILSS 90

Query: 101 HVNDRICDCCDGSDEYDGNTFCPNTCVMGGNIAYKSNNYIS-TTRDVHIVNRKVKEE--I 160
            VND +CDCCDG+DEY+  T C NTC   G    +S   ++  TR+   + + + EE   
Sbjct: 91  RVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEKESLQQLAEVTREGFRLKKILIEEWKT 150

Query: 161 TKEDLFQKLMGLK 171
            +E+   KL+ L+
Sbjct: 151 AREEKQSKLLELQ 163

[Close]

BLAST of MS022358.1 vs. ExPASy Swiss-Prot
Match: P14314 (Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 1.5e-24
Identity = 46/86 (53.49%), Postives = 59/86 (68.60%), Query Frame = 0


Query: 41  YYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSS 100
           Y  S+   C DGS +   D++NDD+CDC DG+DEPGT+AC  G F+C N G  P +I S+
Sbjct: 31  YDESKPFTCLDGSATIPFDQVNDDYCDCKDGSDEPGTAACPNGSFHCTNTGYKPLYIPSN 90

Query: 101 HVNDRICDCCDGSDEYDGNTFCPNTC 127
            VND +CDCCDG+DEY+    C NTC
Sbjct: 91  RVNDGVCDCCDGTDEYNSGVICENTC 116

[Close]

BLAST of MS022358.1 vs. ExPASy TrEMBL
Match: A0A6J1C765 (glucosidase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111008003 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.9e-110
Identity = 198/201 (98.51%), Postives = 199/201 (99.00%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA RPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEAGRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGNIAYKSNNYISTT+DVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT
Sbjct: 121 FCPNTCVMGGNIAYKSNNYISTTQDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
           SFAVFIWVNRCRARSKRRRHR
Sbjct: 181 SFAVFIWVNRCRARSKRRRHR 201

[Close]

BLAST of MS022358.1 vs. ExPASy TrEMBL
Match: A0A6J1GLM3 (glucosidase 2 subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111455090 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.3e-97
Identity = 172/201 (85.57%), Postives = 185/201 (92.04%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA   PKS SFV ++S CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEAGHSPKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+F+FSS VND ICDCCDGSDEYDGN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN AYK++ YIST+RDV ++NRKVKEEITKEDLF +L+GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
            FAV IW NRCRA+SKRRRHR
Sbjct: 181 CFAVLIWANRCRAKSKRRRHR 201

[Close]

BLAST of MS022358.1 vs. ExPASy TrEMBL
Match: A0A6J1HVT0 (glucosidase 2 subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111468297 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.2e-94
Identity = 168/199 (84.42%), Postives = 181/199 (90.95%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA    KS SFV ++S CFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 39  MEAGHSTKSMSFVLLLSFCFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 98

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+F+FSS VND ICDCCDGSDEYDGN 
Sbjct: 99  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFLFSSRVNDHICDCCDGSDEYDGNI 158

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN AYK++ YIST+RDV ++NRKVKEEITKEDLF +L+GLKLVI+LQVAL 
Sbjct: 159 FCPNTCVMGGNAAYKNSYYISTSRDVDVINRKVKEEITKEDLFHRLIGLKLVIILQVALI 218

Query: 181 SFAVFIWVNRCRARSKRRR 200
           SFAV IW NRCR +SKRRR
Sbjct: 219 SFAVIIWANRCRVKSKRRR 237

[Close]

BLAST of MS022358.1 vs. ExPASy TrEMBL
Match: A0A1S4E0C8 (glucosidase 2 subunit beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495629 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 7.1e-94
Identity = 172/201 (85.57%), Postives = 183/201 (91.04%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA R PKSTSFVS++SIC FF SVR+ PS +GVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEA-RSPKSTSFVSLLSICLFFTSVRAAPSFLGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VND ICDCCDGSDEY+GN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN+ YKSNN +STTRDV IV RKVKEEITKEDLFQKL GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNM-YKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
            FA+ IW NRCR +SKRRRHR
Sbjct: 181 IFAILIWANRCRVKSKRRRHR 199

[Close]

BLAST of MS022358.1 vs. ExPASy TrEMBL
Match: A0A5D3BIQ3 (Glucosidase 2 subunit beta isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold786G00030 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 7.1e-94
Identity = 172/201 (85.57%), Postives = 183/201 (91.04%), Query Frame = 0


Query: 1   MEARRPPKSTSFVSMISICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60
           MEA R PKSTSFVS++SIC FF SVR+ PS +GVHPLDEKYYSSEVIKCKDGSRSFTIDR
Sbjct: 1   MEA-RSPKSTSFVSLLSICLFFTSVRAAPSFLGVHPLDEKYYSSEVIKCKDGSRSFTIDR 60

Query: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNT 120
           LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTP+FIFSS VND ICDCCDGSDEY+GN 
Sbjct: 61  LNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPRFIFSSRVNDHICDCCDGSDEYEGNI 120

Query: 121 FCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLKLVIVLQVALT 180
           FCPNTCVMGGN+ YKSNN +STTRDV IV RKVKEEITKEDLFQKL GLKLVI+LQVALT
Sbjct: 121 FCPNTCVMGGNM-YKSNNDVSTTRDVDIVIRKVKEEITKEDLFQKLTGLKLVIILQVALT 180

Query: 181 SFAVFIWVNRCRARSKRRRHR 202
            FA+ IW NRCR +SKRRRHR
Sbjct: 181 IFAILIWANRCRVKSKRRRHR 199

[Close]

BLAST of MS022358.1 vs. TAIR 10
Match: AT2G42390.1 (protein kinase C substrate, heavy chain-related )

HSP 1 Score: 223.4 bits (568), Expect = 1.6e-58
Identity = 110/194 (56.70%), Postives = 138/194 (71.13%), Query Frame = 0


Query: 17  SICFFFASVRSVPSLIGVHPLDEKYYSSEVIKCKDGSRSFTIDRLNDDFCDCVDGTDEPG 76
           S+     SV S   L+GVHPLDEKY+ S+VIKCKDGS+SFT DRLND+FCDC+DGTDEPG
Sbjct: 11  SLAVVVISVASTSPLVGVHPLDEKYFDSDVIKCKDGSKSFTRDRLNDNFCDCLDGTDEPG 70

Query: 77  TSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGNTFCPNTCVMGGNIAYKS 136
           TSAC  GKFYCRN+GS+P+F++SS VNDRICDCCDGSDEY+ +  CPNTC+MGGN+    
Sbjct: 71  TSACPNGKFYCRNIGSSPKFVYSSRVNDRICDCCDGSDEYESSIHCPNTCIMGGNV---- 130

Query: 137 NNYI----STTRDVHI---VNRKVKEEIT---KEDLFQKLMGLKLVIVLQVALTSFAVFI 196
            NYI    +  + +H+        KE  T    +D+ + L G+KLV  LQ+    F V +
Sbjct: 131 -NYIYKPRANLKSIHLQLGSTPHPKEFYTIGNLQDMVKNLQGMKLVFALQMVFIGFLVIL 190

Query: 197 WVNRCRARSKRRRH 201
           W+ R RARSKRRR+
Sbjct: 191 WMLRSRARSKRRRY 199

[Close]

BLAST of MS022358.1 vs. TAIR 10
Match: AT5G56360.1 (calmodulin-binding protein )

HSP 1 Score: 157.9 bits (398), Expect = 8.4e-39
Identity = 89/171 (52.05%), Postives = 107/171 (62.57%), Query Frame = 0


Query: 10  TSFVSM---ISICFFFAS-VRSVPS-----LIGVHPLDEKYY-SSEVIKCKDGSRSFTID 69
           +SFVS+   +S     AS +RS  S      +G+ P DEKYY SS  IKCKDGS+ FT  
Sbjct: 7   SSFVSVSLQLSFLLLLASAIRSSSSPPNDPFLGISPQDEKYYKSSSEIKCKDGSKKFTKA 66

Query: 70  RLNDDFCDCVDGTDEPGTSACARGKFYCRNMGSTPQFIFSSHVNDRICDCCDGSDEYDGN 129
           +LNDDFCDC DGTDEPGTSAC  GKFYCRN G +P  +FSS VND ICDCCDGSDEYDG+
Sbjct: 67  QLNDDFCDCSDGTDEPGTSACPTGKFYCRNAGHSPVILFSSRVNDGICDCCDGSDEYDGH 126

Query: 130 TFCPNTCVMGGNIAYKSNNYISTTRDVHIVNRKVKEEITKEDLFQKLMGLK 171
             C NTC   G  A ++      T +  +V R+ + E  K  L +    LK
Sbjct: 127 VSCQNTCWEAGKAARENLKKKIETYNQGLVIRRQEIEQAKVGLEKDAAELK 177

The following BLAST results are available for this feature:

BLAST of MS022358.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136263.1	3.8e-110	98.51	glucosidase 2 subunit beta [Momordica charantia]	[more]
XP_038887466.1	4.9e-97	87.13	glucosidase 2 subunit beta [Benincasa hispida]	[more]
XP_022952390.1	1.1e-96	85.57	glucosidase 2 subunit beta [Cucurbita moschata]	[more]
XP_023554618.1	3.2e-96	85.07	glucosidase 2 subunit beta [Cucurbita pepo subsp. pepo]	[more]
XP_022969237.1	8.6e-94	84.42	glucosidase 2 subunit beta [Cucurbita maxima]	[more]

BLAST of MS022358.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FM96	1.2e-37	52.05	Glucosidase 2 subunit beta OS=Arabidopsis thaliana OX=3702 GN=PSL4 PE=2 SV=1	[more]
A2WNF5	1.1e-35	61.26	Glucosidase 2 subunit beta OS=Oryza sativa subsp. indica OX=39946 GN=OsI_01383 P...	[more]
Q5NBP9	1.1e-35	61.26	Glucosidase 2 subunit beta OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0276...	[more]
O08795	2.3e-25	42.86	Glucosidase 2 subunit beta OS=Mus musculus OX=10090 GN=Prkcsh PE=1 SV=1	[more]
P14314	1.5e-24	53.49	Glucosidase 2 subunit beta OS=Homo sapiens OX=9606 GN=PRKCSH PE=1 SV=2	[more]

BLAST of MS022358.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C765	1.9e-110	98.51	glucosidase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111008003 PE=4 S...	[more]
A0A6J1GLM3	5.3e-97	85.57	glucosidase 2 subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111455090 PE=4 SV...	[more]
A0A6J1HVT0	4.2e-94	84.42	glucosidase 2 subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111468297 PE=4 SV=1	[more]
A0A1S4E0C8	7.1e-94	85.57	glucosidase 2 subunit beta isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495629 PE...	[more]
A0A5D3BIQ3	7.1e-94	85.57	Glucosidase 2 subunit beta isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of MS022358.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42390.1	1.6e-58	56.70	protein kinase C substrate, heavy chain-related	[more]
AT5G56360.1	8.4e-39	52.05	calmodulin-binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR028146	Glucosidase II beta subunit, N-terminal	PFAM	PF12999	PRKCSH-like	coord: 13..130 e-value: 4.4E-32 score: 111.3
IPR039794	Glucosidase II beta subunit-like	PANTHER	PTHR12630	N-LINKED OLIGOSACCHARIDE PROCESSING	coord: 19..199
None	No IPR available	PANTHER	PTHR12630:SF17	EXPRESSED PROTEIN	coord: 19..199

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS022358	MS022358	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS022358.1-cds	MS022358.1-cds-scaffold47:2771367..2771478	CDS
MS022358.1-cds	MS022358.1-cds-scaffold47:2771681..2771794	CDS
MS022358.1-cds	MS022358.1-cds-scaffold47:2772462..2772557	CDS
MS022358.1-cds	MS022358.1-cds-scaffold47:2773038..2773217	CDS
MS022358.1-cds	MS022358.1-cds-scaffold47:2773297..2773397	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS022358.1	MS022358.1-protein	polypeptide