MS022185.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS022185.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	thioredoxin-like protein CDSP32, chloroplastic
Location	scaffold47: 1499463 .. 1500152 (+)
Sequence length	690
RNA-Seq Expression	MS022185.1
Synteny	MS022185.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTCAAGTTTCCATAAAGTACAAGAAGTTCACAGCATTACAGAATTTGAAACCGCTCTTATCCAGGCCAAGGATAGCCTCGTCCTGGTCGAGTTTATCCCCACCGCCCGCAGCCAAACACCCCCTCTCACCGTCCAACTCAGCCAGAGCCAGCAAAACGACGGCGTTTGCTTCATGTTGGTAAAGGCCGACGAATCGGAAGAAACTAAAGCGCTGTGCAGAAGAGAGAGCGTGAAAAGAATTCCACACTACAATTTTTACAAGAACGAAAAGAAACTCCTTGAAAAATCGAGGCTGAAGGCGGAGGAATTTATCCGGTACGCGCAGTACTACGGGAACTCCCATGGGTCCGTAATCCAGCTCCGTTGTATGGACGACGTGGATAGGCTTATGATGGCTGATCGGACGGCTGCGGGTTGTGGAAAGGTTTTAGTGTTGAATTTGGGCCTGAGAAATTGCGGGCCGTGCGTGAAGATTTATCCGACAATAGTTCGGCTGTCGGCCCAGATGCGAGACGACGTCGTTTTCGCGAGATTGATTGGAGACGACAATGACGGTCGGAATATGGAGTTTTTAAGGAACAATTCTGTTGTTCGAGTGCCTGCTTTCTTGTTTATTAGAAATGGGGAGATTCTTGGAAGCTATGTGGGTTCTCAGAAAGCTTTACTTGTGAAGGAGATATTTGGATAC

mRNA sequence

CCCTCAAGTTTCCATAAAGTACAAGAAGTTCACAGCATTACAGAATTTGAAACCGCTCTTATCCAGGCCAAGGATAGCCTCGTCCTGGTCGAGTTTATCCCCACCGCCCGCAGCCAAACACCCCCTCTCACCGTCCAACTCAGCCAGAGCCAGCAAAACGACGGCGTTTGCTTCATGTTGGTAAAGGCCGACGAATCGGAAGAAACTAAAGCGCTGTGCAGAAGAGAGAGCGTGAAAAGAATTCCACACTACAATTTTTACAAGAACGAAAAGAAACTCCTTGAAAAATCGAGGCTGAAGGCGGAGGAATTTATCCGGTACGCGCAGTACTACGGGAACTCCCATGGGTCCGTAATCCAGCTCCGTTGTATGGACGACGTGGATAGGCTTATGATGGCTGATCGGACGGCTGCGGGTTGTGGAAAGGTTTTAGTGTTGAATTTGGGCCTGAGAAATTGCGGGCCGTGCGTGAAGATTTATCCGACAATAGTTCGGCTGTCGGCCCAGATGCGAGACGACGTCGTTTTCGCGAGATTGATTGGAGACGACAATGACGGTCGGAATATGGAGTTTTTAAGGAACAATTCTGTTGTTCGAGTGCCTGCTTTCTTGTTTATTAGAAATGGGGAGATTCTTGGAAGCTATGTGGGTTCTCAGAAAGCTTTACTTGTGAAGGAGATATTTGGATAC

Coding sequence (CDS)

CCCTCAAGTTTCCATAAAGTACAAGAAGTTCACAGCATTACAGAATTTGAAACCGCTCTTATCCAGGCCAAGGATAGCCTCGTCCTGGTCGAGTTTATCCCCACCGCCCGCAGCCAAACACCCCCTCTCACCGTCCAACTCAGCCAGAGCCAGCAAAACGACGGCGTTTGCTTCATGTTGGTAAAGGCCGACGAATCGGAAGAAACTAAAGCGCTGTGCAGAAGAGAGAGCGTGAAAAGAATTCCACACTACAATTTTTACAAGAACGAAAAGAAACTCCTTGAAAAATCGAGGCTGAAGGCGGAGGAATTTATCCGGTACGCGCAGTACTACGGGAACTCCCATGGGTCCGTAATCCAGCTCCGTTGTATGGACGACGTGGATAGGCTTATGATGGCTGATCGGACGGCTGCGGGTTGTGGAAAGGTTTTAGTGTTGAATTTGGGCCTGAGAAATTGCGGGCCGTGCGTGAAGATTTATCCGACAATAGTTCGGCTGTCGGCCCAGATGCGAGACGACGTCGTTTTCGCGAGATTGATTGGAGACGACAATGACGGTCGGAATATGGAGTTTTTAAGGAACAATTCTGTTGTTCGAGTGCCTGCTTTCTTGTTTATTAGAAATGGGGAGATTCTTGGAAGCTATGTGGGTTCTCAGAAAGCTTTACTTGTGAAGGAGATATTTGGATAC

Protein sequence

PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCFMLVKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY

Homology

[Close]

BLAST of MS022185.1 vs. NCBI nr
Match: XP_022135917.1 (thioredoxin-like protein CDSP32, chloroplastic [Momordica charantia])

HSP 1 Score: 442.6 bits (1137), Expect = 2.1e-120
Identity = 222/230 (96.52%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 1   PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCFML 60
           PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCF+L
Sbjct: 44  PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCFVL 103

Query: 61  VKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQ 120
           VKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFI+Y QYYGNSHGSVIQ
Sbjct: 104 VKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIQYEQYYGNSHGSVIQ 163

Query: 121 LRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLI 180
           LRCMDDVDRLMMADRTAAGCGKVLVLNLGL  CGPCVKIYPTIV LSAQMRDDVVFARLI
Sbjct: 164 LRCMDDVDRLMMADRTAAGCGKVLVLNLGLSTCGPCVKIYPTIVWLSAQMRDDVVFARLI 223

Query: 181 GDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           GDDNDGRNMEFLRNN VVRVPAFLFIRNGE+LGSYVGSQKALLVKEIFGY
Sbjct: 224 GDDNDGRNMEFLRNNYVVRVPAFLFIRNGELLGSYVGSQKALLVKEIFGY 273

[Close]

BLAST of MS022185.1 vs. NCBI nr
Match: KAF3517967.1 (hypothetical protein DY000_02064034 [Brassica cretica])

HSP 1 Score: 208.4 bits (529), Expect = 6.7e-50
Identity = 110/228 (48.25%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFMLVK 65
           KVQ++HS  EF+ AL +AK+ LV+VEF  +   Q+    P  V+LS++  ND V F+LV 
Sbjct: 78  KVQKIHSGEEFDEALKKAKNKLVVVEFATSKSDQSNKIYPFMVELSRA-CND-VDFLLVM 137

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DESE+TK LCRRE ++++PH++FYK+ +K+ E+  +  ++ +    YYG+SH +V+QL 
Sbjct: 138 GDESEKTKELCRREKIEKVPHFSFYKSMEKIHEEEGIGPDQLMGDVLYYGDSHSAVVQLH 197

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFAR+ GD
Sbjct: 198 GRADVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFARMNGD 257

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFL++ +V+ VP FLFIRNG+I G YVGS K  L+ EI  Y
Sbjct: 258 ENDS-CMEFLKDMNVIEVPTFLFIRNGDICGRYVGSGKGELIGEILRY 299

[Close]

BLAST of MS022185.1 vs. NCBI nr
Match: XP_013591845.1 (PREDICTED: thioredoxin-like protein CDSP32, chloroplastic [Brassica oleracea var. oleracea] >VDD64588.1 unnamed protein product [Brassica oleracea])

HSP 1 Score: 208.4 bits (529), Expect = 6.7e-50
Identity = 110/228 (48.25%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFMLVK 65
           KVQ++HS  EF+ AL +AK+ LV+VEF  +   Q+    P  V+LS++  ND V F+LV 
Sbjct: 78  KVQKIHSGEEFDEALKKAKNKLVVVEFATSKSDQSNKIYPFMVELSRA-CND-VDFLLVM 137

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DESE+TK LCRRE ++++PH++FYK+ +K+ E+  +  ++ +    YYG+SH +V+QL 
Sbjct: 138 GDESEKTKELCRREKIEKVPHFSFYKSMEKIHEEEGIGPDQLMGDVLYYGDSHSAVVQLH 197

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFAR+ GD
Sbjct: 198 GRADVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFARMNGD 257

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFL++ +V+ VP FLFIRNG+I G YVGS K  L+ EI  Y
Sbjct: 258 ENDS-CMEFLKDMNVIEVPTFLFIRNGDICGRYVGSGKGELIGEILRY 299

[Close]

BLAST of MS022185.1 vs. NCBI nr
Match: RWR84511.1 (thioredoxin-like protein CDSP32, chloroplastic [Cinnamomum micranthum f. kanehirae])

HSP 1 Score: 208.4 bits (529), Expect = 6.7e-50
Identity = 113/231 (48.92%), Postives = 160/231 (69.26%), Query Frame = 0


Query: 3   SFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFM 62
           S  +VQ+VHSI EF+ AL  AK+ LV+VE+  +    +    P  V LS++  ND V F+
Sbjct: 69  SDERVQQVHSIDEFDAALQTAKNKLVVVEYAASHSYHSRKIYPFMVDLSRT-CND-VEFL 128

Query: 63  LVKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVI 122
           LV  DESEETKALC RE + ++PH++FYK+ +K+ E+  +  +E I    YYG++H +V+
Sbjct: 129 LVMGDESEETKALCAREKIDKVPHFSFYKSMEKIHEEEGIGPDELIGDVLYYGDNHSAVV 188

Query: 123 QLRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARL 182
           QL C +DV+ L+   +     GK++VL++GL++CGPCVK+YPT+V+LS QM D VVFAR+
Sbjct: 189 QLHCREDVEALIDVQQKD---GKLIVLDVGLKHCGPCVKVYPTVVKLSKQMTDTVVFARM 248

Query: 183 IGDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
            GD+ND   M+FL++  VV VP FLFI++GEI G YVGS K  L+ EI  Y
Sbjct: 249 NGDENDS-CMQFLKDMDVVEVPTFLFIKDGEISGRYVGSGKGELIGEILRY 293

[Close]

BLAST of MS022185.1 vs. NCBI nr
Match: KAF2607574.1 (hypothetical protein F2Q68_00046524 [Brassica cretica])

HSP 1 Score: 208.0 bits (528), Expect = 8.7e-50
Identity = 109/228 (47.81%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFMLVK 65
           KVQ++HS  EF+ AL +AK+ LV+VEF  +   Q+    P  V+LS++  ND V F+LV 
Sbjct: 78  KVQKIHSGEEFDEALKKAKNKLVVVEFATSKSDQSNKIYPFMVELSRA-CND-VDFLLVM 137

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DESE+TK LCRRE ++++PH++FYK+ +K+ E+  +  ++ +    YYG+SH +V+QL 
Sbjct: 138 GDESEKTKELCRREKIEKVPHFSFYKSMEKIHEEEGIGPDQLMGDVLYYGDSHSAVVQLH 197

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + +VFAR+ GD
Sbjct: 198 GRADVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETIVFARMNGD 257

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFL++ +V+ VP FLFIRNG+I G YVGS K  L+ EI  Y
Sbjct: 258 ENDS-CMEFLKDMNVIEVPTFLFIRNGDICGRYVGSGKGELIGEILRY 299

[Close]

BLAST of MS022185.1 vs. ExPASy Swiss-Prot
Match: Q9SGS4 (Thioredoxin-like protein CDSP32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CDSP32 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.3e-52
Identity = 107/233 (45.92%), Postives = 164/233 (70.39%), Query Frame = 0


Query: 1   PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVC 60
           P++  KVQ++HS  EF+ AL  AK  LV+ EF  +   Q+    P  V+LS++  ND V 
Sbjct: 69  PANDEKVQKIHSGEEFDVALKNAKSKLVVAEFATSKSDQSNKIYPFMVELSRT-CND-VV 128

Query: 61  FMLVKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGS 120
           F+LV  DES++T+ LCRRE ++++PH++FYK+ +K+ E+  ++ ++ +    YYG++H +
Sbjct: 129 FLLVMGDESDKTRELCRREKIEKVPHFSFYKSMEKIHEEEGIEPDQLMGDVLYYGDNHSA 188

Query: 121 VIQLRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFA 180
           V+QL    DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFA
Sbjct: 189 VVQLHGRPDVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFA 248

Query: 181 RLIGDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           R+ GD+ND   MEFL++ +V+ VP FLFIR+GEI G YVGS K  L+ EI  Y
Sbjct: 249 RMNGDENDS-CMEFLKDMNVIEVPTFLFIRDGEIRGRYVGSGKGELIGEILRY 295

[Close]

BLAST of MS022185.1 vs. ExPASy Swiss-Prot
Match: Q84NN4 (Thioredoxin-like protein CDSP32, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CDSP32 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-46
Identity = 104/228 (45.61%), Postives = 149/228 (65.35%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPT---ARSQTPPLTVQLSQSQQNDGVCFMLVK 65
           +V +VHS  E + AL  AK+ LV+VEF  +     S+  P  V+LS++  +  V F+LV 
Sbjct: 73  RVVQVHSAEELDGALRAAKERLVVVEFAASHSVNSSRIYPCMVELSRTCGD--VDFLLVM 132

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DES+ T+ LCRRE +  +PH+ FYK  +K+ E+  +  ++      YYG+ H +V+QL 
Sbjct: 133 GDESDATRELCRREGITAVPHFTFYKGAEKVHEEEGIGPDQLAGDVLYYGDHHSAVVQLH 192

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV+ L ++D    G GK++VL++GL+ CGPCVK+YPT+V+LS  M D  VFAR+ GD
Sbjct: 193 SRADVESL-ISDHRGEG-GKLVVLDVGLKRCGPCVKVYPTVVKLSRTMADTTVFARMNGD 252

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFLR+  VV VP FLFIR+G+I+G YVGS +  L+ EI  Y
Sbjct: 253 ENDS-CMEFLRDMDVVEVPTFLFIRDGDIVGRYVGSGRGELIGEILRY 295

[Close]

BLAST of MS022185.1 vs. ExPASy Swiss-Prot
Match: Q8IFW4 (Thioredoxin-T OS=Drosophila melanogaster OX=7227 GN=TrxT PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.3e-05
Identity = 33/109 (30.28%), Postives = 58/109 (53.21%), Query Frame = 0


Query: 118 VIQLRCMDDVD-RLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVF 177
           V  +R  DD+D +L++A+       K++V++     CGPC  I P +  L+ +  D VV 
Sbjct: 2   VYPVRNKDDLDQQLILAE------DKLVVIDFYADWCGPCKIIAPKLDELAHEYSDRVVV 61

Query: 178 ARLIGDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVK 226
            ++  D+N+   +E+    +V  +P F+FI+ G +L  +VG     L K
Sbjct: 62  LKVNVDENEDITVEY----NVNSMPTFVFIKGGNVLELFVGCNSDKLAK 100

[Close]

BLAST of MS022185.1 vs. ExPASy Swiss-Prot
Match: Q9C9Y6 (Thioredoxin H9 OS=Arabidopsis thaliana OX=3702 GN=TRX9 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.3e-05
Identity = 30/100 (30.00%), Postives = 50/100 (50.00%), Query Frame = 0


Query: 128 DRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGDDNDGR 187
           D+L  ADR     GK++V N     CGPC  + P  + LS +    ++F  +  D+    
Sbjct: 36  DKLAEADRD----GKIVVANFSATWCGPCKIVAPFFIELS-EKHSSLMFLLVDVDELS-- 95

Query: 188 NMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEI 228
             +F  +  +   P F F++NG+ +G  VG+ K  L K++
Sbjct: 96  --DFSSSWDIKATPTFFFLKNGQQIGKLVGANKPELQKKV 126

[Close]

BLAST of MS022185.1 vs. ExPASy Swiss-Prot
Match: Q9XIF4 (Thioredoxin H7 OS=Arabidopsis thaliana OX=3702 GN=TRX7 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 8.9e-05
Identity = 31/89 (34.83%), Postives = 47/89 (52.81%), Query Frame = 0


Query: 139 GCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGDDNDGRNMEFLRNNSVV 198
           G  K+LV++     CGPC  + P  VR  A    + VFAR+  D    R M+       +
Sbjct: 41  GSNKLLVIDFTAVWCGPCKAMEPR-VREIASKYSEAVFARVDVD----RLMDVAGTYRAI 100

Query: 199 RVPAFLFIRNGEILGSYVGSQKALLVKEI 228
            +PAF+F++ GE +   VG++   LVK+I
Sbjct: 101 TLPAFVFVKRGEEIDRVVGAKPDELVKKI 124

[Close]

BLAST of MS022185.1 vs. ExPASy TrEMBL
Match: A0A6J1C444 (thioredoxin-like protein CDSP32, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007755 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.0e-120
Identity = 222/230 (96.52%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 1   PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCFML 60
           PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCF+L
Sbjct: 44  PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTPPLTVQLSQSQQNDGVCFVL 103

Query: 61  VKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQ 120
           VKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFI+Y QYYGNSHGSVIQ
Sbjct: 104 VKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIQYEQYYGNSHGSVIQ 163

Query: 121 LRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLI 180
           LRCMDDVDRLMMADRTAAGCGKVLVLNLGL  CGPCVKIYPTIV LSAQMRDDVVFARLI
Sbjct: 164 LRCMDDVDRLMMADRTAAGCGKVLVLNLGLSTCGPCVKIYPTIVWLSAQMRDDVVFARLI 223

Query: 181 GDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           GDDNDGRNMEFLRNN VVRVPAFLFIRNGE+LGSYVGSQKALLVKEIFGY
Sbjct: 224 GDDNDGRNMEFLRNNYVVRVPAFLFIRNGELLGSYVGSQKALLVKEIFGY 273

[Close]

BLAST of MS022185.1 vs. ExPASy TrEMBL
Match: A0A3P6GEG8 (Thioredoxin domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC6T40041H PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 3.2e-50
Identity = 110/228 (48.25%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFMLVK 65
           KVQ++HS  EF+ AL +AK+ LV+VEF  +   Q+    P  V+LS++  ND V F+LV 
Sbjct: 78  KVQKIHSGEEFDEALKKAKNKLVVVEFATSKSDQSNKIYPFMVELSRA-CND-VDFLLVM 137

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DESE+TK LCRRE ++++PH++FYK+ +K+ E+  +  ++ +    YYG+SH +V+QL 
Sbjct: 138 GDESEKTKELCRREKIEKVPHFSFYKSMEKIHEEEGIGPDQLMGDVLYYGDSHSAVVQLH 197

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFAR+ GD
Sbjct: 198 GRADVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFARMNGD 257

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFL++ +V+ VP FLFIRNG+I G YVGS K  L+ EI  Y
Sbjct: 258 ENDS-CMEFLKDMNVIEVPTFLFIRNGDICGRYVGSGKGELIGEILRY 299

[Close]

BLAST of MS022185.1 vs. ExPASy TrEMBL
Match: A0A0D3D116 (Thioredoxin domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 GN=106300282 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 3.2e-50
Identity = 110/228 (48.25%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFMLVK 65
           KVQ++HS  EF+ AL +AK+ LV+VEF  +   Q+    P  V+LS++  ND V F+LV 
Sbjct: 78  KVQKIHSGEEFDEALKKAKNKLVVVEFATSKSDQSNKIYPFMVELSRA-CND-VDFLLVM 137

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DESE+TK LCRRE ++++PH++FYK+ +K+ E+  +  ++ +    YYG+SH +V+QL 
Sbjct: 138 GDESEKTKELCRREKIEKVPHFSFYKSMEKIHEEEGIGPDQLMGDVLYYGDSHSAVVQLH 197

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFAR+ GD
Sbjct: 198 GRADVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFARMNGD 257

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFL++ +V+ VP FLFIRNG+I G YVGS K  L+ EI  Y
Sbjct: 258 ENDS-CMEFLKDMNVIEVPTFLFIRNGDICGRYVGSGKGELIGEILRY 299

[Close]

BLAST of MS022185.1 vs. ExPASy TrEMBL
Match: A0A3N6QK12 (Thioredoxin domain-containing protein OS=Brassica cretica OX=69181 GN=DY000_00013435 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 3.2e-50
Identity = 110/228 (48.25%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 6   KVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFMLVK 65
           KVQ++HS  EF+ AL +AK+ LV+VEF  +   Q+    P  V+LS++  ND V F+LV 
Sbjct: 78  KVQKIHSGEEFDEALKKAKNKLVVVEFATSKSDQSNKIYPFMVELSRA-CND-VDFLLVM 137

Query: 66  ADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVIQLR 125
            DESE+TK LCRRE ++++PH++FYK+ +K+ E+  +  ++ +    YYG+SH +V+QL 
Sbjct: 138 GDESEKTKELCRREKIEKVPHFSFYKSMEKIHEEEGIGPDQLMGDVLYYGDSHSAVVQLH 197

Query: 126 CMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGD 185
              DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFAR+ GD
Sbjct: 198 GRADVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFARMNGD 257

Query: 186 DNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           +ND   MEFL++ +V+ VP FLFIRNG+I G YVGS K  L+ EI  Y
Sbjct: 258 ENDS-CMEFLKDMNVIEVPTFLFIRNGDICGRYVGSGKGELIGEILRY 299

[Close]

BLAST of MS022185.1 vs. ExPASy TrEMBL
Match: A0A3S3NQJ3 (Thioredoxin-like protein CDSP32, chloroplastic OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_01332800 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 3.2e-50
Identity = 113/231 (48.92%), Postives = 160/231 (69.26%), Query Frame = 0


Query: 3   SFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVCFM 62
           S  +VQ+VHSI EF+ AL  AK+ LV+VE+  +    +    P  V LS++  ND V F+
Sbjct: 69  SDERVQQVHSIDEFDAALQTAKNKLVVVEYAASHSYHSRKIYPFMVDLSRT-CND-VEFL 128

Query: 63  LVKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGSVI 122
           LV  DESEETKALC RE + ++PH++FYK+ +K+ E+  +  +E I    YYG++H +V+
Sbjct: 129 LVMGDESEETKALCAREKIDKVPHFSFYKSMEKIHEEEGIGPDELIGDVLYYGDNHSAVV 188

Query: 123 QLRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARL 182
           QL C +DV+ L+   +     GK++VL++GL++CGPCVK+YPT+V+LS QM D VVFAR+
Sbjct: 189 QLHCREDVEALIDVQQKD---GKLIVLDVGLKHCGPCVKVYPTVVKLSKQMTDTVVFARM 248

Query: 183 IGDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
            GD+ND   M+FL++  VV VP FLFI++GEI G YVGS K  L+ EI  Y
Sbjct: 249 NGDENDS-CMQFLKDMDVVEVPTFLFIKDGEISGRYVGSGKGELIGEILRY 293

[Close]

BLAST of MS022185.1 vs. TAIR 10
Match: AT1G76080.1 (chloroplastic drought-induced stress protein of 32 kD )

HSP 1 Score: 206.5 bits (524), Expect = 2.4e-53
Identity = 107/233 (45.92%), Postives = 164/233 (70.39%), Query Frame = 0


Query: 1   PSSFHKVQEVHSITEFETALIQAKDSLVLVEFIPTARSQTP---PLTVQLSQSQQNDGVC 60
           P++  KVQ++HS  EF+ AL  AK  LV+ EF  +   Q+    P  V+LS++  ND V 
Sbjct: 69  PANDEKVQKIHSGEEFDVALKNAKSKLVVAEFATSKSDQSNKIYPFMVELSRT-CND-VV 128

Query: 61  FMLVKADESEETKALCRRESVKRIPHYNFYKNEKKLLEKSRLKAEEFIRYAQYYGNSHGS 120
           F+LV  DES++T+ LCRRE ++++PH++FYK+ +K+ E+  ++ ++ +    YYG++H +
Sbjct: 129 FLLVMGDESDKTRELCRREKIEKVPHFSFYKSMEKIHEEEGIEPDQLMGDVLYYGDNHSA 188

Query: 121 VIQLRCMDDVDRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFA 180
           V+QL    DV++L+  +RT    GK++VL++GL++CGPCVK+YPT+++LS  M + VVFA
Sbjct: 189 VVQLHGRPDVEKLIDENRTG---GKLIVLDVGLKHCGPCVKVYPTVLKLSRSMSETVVFA 248

Query: 181 RLIGDDNDGRNMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEIFGY 231
           R+ GD+ND   MEFL++ +V+ VP FLFIR+GEI G YVGS K  L+ EI  Y
Sbjct: 249 RMNGDENDS-CMEFLKDMNVIEVPTFLFIRDGEIRGRYVGSGKGELIGEILRY 295

[Close]

BLAST of MS022185.1 vs. TAIR 10
Match: AT3G08710.1 (thioredoxin H-type 9 )

HSP 1 Score: 50.8 bits (120), Expect = 1.7e-06
Identity = 30/100 (30.00%), Postives = 50/100 (50.00%), Query Frame = 0


Query: 128 DRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGDDNDGR 187
           D+L  ADR     GK++V N     CGPC  + P  + LS +    ++F  +  D+    
Sbjct: 36  DKLAEADRD----GKIVVANFSATWCGPCKIVAPFFIELS-EKHSSLMFLLVDVDELS-- 95

Query: 188 NMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEI 228
             +F  +  +   P F F++NG+ +G  VG+ K  L K++
Sbjct: 96  --DFSSSWDIKATPTFFFLKNGQQIGKLVGANKPELQKKV 126

[Close]

BLAST of MS022185.1 vs. TAIR 10
Match: AT3G08710.2 (thioredoxin H-type 9 )

HSP 1 Score: 50.8 bits (120), Expect = 1.7e-06
Identity = 30/100 (30.00%), Postives = 50/100 (50.00%), Query Frame = 0


Query: 128 DRLMMADRTAAGCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGDDNDGR 187
           D+L  ADR     GK++V N     CGPC  + P  + LS +    ++F  +  D+    
Sbjct: 36  DKLAEADRD----GKIVVANFSATWCGPCKIVAPFFIELS-EKHSSLMFLLVDVDELS-- 95

Query: 188 NMEFLRNNSVVRVPAFLFIRNGEILGSYVGSQKALLVKEI 228
             +F  +  +   P F F++NG+ +G  VG+ K  L K++
Sbjct: 96  --DFSSSWDIKATPTFFFLKNGQQIGKLVGANKPELQKKV 126

[Close]

BLAST of MS022185.1 vs. TAIR 10
Match: AT1G59730.1 (thioredoxin H-type 7 )

HSP 1 Score: 48.9 bits (115), Expect = 6.3e-06
Identity = 31/89 (34.83%), Postives = 47/89 (52.81%), Query Frame = 0


Query: 139 GCGKVLVLNLGLRNCGPCVKIYPTIVRLSAQMRDDVVFARLIGDDNDGRNMEFLRNNSVV 198
           G  K+LV++     CGPC  + P  VR  A    + VFAR+  D    R M+       +
Sbjct: 41  GSNKLLVIDFTAVWCGPCKAMEPR-VREIASKYSEAVFARVDVD----RLMDVAGTYRAI 100

Query: 199 RVPAFLFIRNGEILGSYVGSQKALLVKEI 228
            +PAF+F++ GE +   VG++   LVK+I
Sbjct: 101 TLPAFVFVKRGEEIDRVVGAKPDELVKKI 124

The following BLAST results are available for this feature:

BLAST of MS022185.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135917.1	2.1e-120	96.52	thioredoxin-like protein CDSP32, chloroplastic [Momordica charantia]	[more]
KAF3517967.1	6.7e-50	48.25	hypothetical protein DY000_02064034 [Brassica cretica]	[more]
XP_013591845.1	6.7e-50	48.25	PREDICTED: thioredoxin-like protein CDSP32, chloroplastic [Brassica oleracea var...	[more]
RWR84511.1	6.7e-50	48.92	thioredoxin-like protein CDSP32, chloroplastic [Cinnamomum micranthum f. kanehir...	[more]
KAF2607574.1	8.7e-50	47.81	hypothetical protein F2Q68_00046524 [Brassica cretica]	[more]

BLAST of MS022185.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SGS4	3.3e-52	45.92	Thioredoxin-like protein CDSP32, chloroplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q84NN4	1.2e-46	45.61	Thioredoxin-like protein CDSP32, chloroplastic OS=Oryza sativa subsp. japonica O...	[more]
Q8IFW4	2.3e-05	30.28	Thioredoxin-T OS=Drosophila melanogaster OX=7227 GN=TrxT PE=1 SV=1	[more]
Q9C9Y6	2.3e-05	30.00	Thioredoxin H9 OS=Arabidopsis thaliana OX=3702 GN=TRX9 PE=1 SV=1	[more]
Q9XIF4	8.9e-05	34.83	Thioredoxin H7 OS=Arabidopsis thaliana OX=3702 GN=TRX7 PE=2 SV=1	[more]

BLAST of MS022185.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C444	1.0e-120	96.52	thioredoxin-like protein CDSP32, chloroplastic OS=Momordica charantia OX=3673 GN...	[more]
A0A3P6GEG8	3.2e-50	48.25	Thioredoxin domain-containing protein OS=Brassica oleracea OX=3712 GN=BOLC6T4004...	[more]
A0A0D3D116	3.2e-50	48.25	Thioredoxin domain-containing protein OS=Brassica oleracea var. oleracea OX=1093...	[more]
A0A3N6QK12	3.2e-50	48.25	Thioredoxin domain-containing protein OS=Brassica cretica OX=69181 GN=DY000_0001...	[more]
A0A3S3NQJ3	3.2e-50	48.92	Thioredoxin-like protein CDSP32, chloroplastic OS=Cinnamomum micranthum f. kaneh...	[more]

BLAST of MS022185.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76080.1	2.4e-53	45.92	chloroplastic drought-induced stress protein of 32 kD	[more]
AT3G08710.1	1.7e-06	30.00	thioredoxin H-type 9	[more]
AT3G08710.2	1.7e-06	30.00	thioredoxin H-type 9	[more]
AT1G59730.1	6.3e-06	34.83	thioredoxin H-type 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 2..114 e-value: 2.3E-6 score: 29.2
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 115..228 e-value: 2.3E-13 score: 51.8
IPR013766	Thioredoxin domain	PFAM	PF00085	Thioredoxin	coord: 152..224 e-value: 4.5E-7 score: 29.8
IPR044192	Thioredoxin-like protein CDSP32	PANTHER	PTHR47578	THIOREDOXIN-LIKE PROTEIN CDSP32, CHLOROPLASTIC	coord: 5..230
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 3..100
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 93..224

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS022185	MS022185	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS022185.1-cds	MS022185.1-cds-scaffold47:1499463..1500152	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS022185.1	MS022185.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016671	oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor