MS018775.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS018775.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Defensin
Location	scaffold313: 1880966 .. 1881145 (-)
Sequence length	180
RNA-Seq Expression	MS018775.1
Synteny	MS018775.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGGAGCAGGAATGGCGCCGATGGTTGCAGAGGGAAGGACATGCGAGTCTCAGAGCCGGCACTTCAAGGGGCTATGCTTTAGTAAGAACAACTGTGGCCATGTCTGCAAAACGGAGGGCTTCCACGGCGGCCATTGCAGAGGCTTCCGCCGCCGCTGCTTCTGCACAAAGCACTGTCTT

mRNA sequence

TTGGGAGCAGGAATGGCGCCGATGGTTGCAGAGGGAAGGACATGCGAGTCTCAGAGCCGGCACTTCAAGGGGCTATGCTTTAGTAAGAACAACTGTGGCCATGTCTGCAAAACGGAGGGCTTCCACGGCGGCCATTGCAGAGGCTTCCGCCGCCGCTGCTTCTGCACAAAGCACTGTCTT

Coding sequence (CDS)

TTGGGAGCAGGAATGGCGCCGATGGTTGCAGAGGGAAGGACATGCGAGTCTCAGAGCCGGCACTTCAAGGGGCTATGCTTTAGTAAGAACAACTGTGGCCATGTCTGCAAAACGGAGGGCTTCCACGGCGGCCATTGCAGAGGCTTCCGCCGCCGCTGCTTCTGCACAAAGCACTGTCTT

Protein sequence

LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL

Homology

[Close]

BLAST of MS018775.1 vs. NCBI nr
Match: XP_022146369.1 (defensin-like protein 2 [Momordica charantia])

HSP 1 Score: 134.8 bits (338), Expect = 2.5e-28
Identity = 58/60 (96.67%), Postives = 58/60 (96.67%), Query Frame = 0


Query: 1   LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
           LG GMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGG CRGFRRRCFCTKHCL
Sbjct: 87  LGTGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGRCRGFRRRCFCTKHCL 146

[Close]

BLAST of MS018775.1 vs. NCBI nr
Match: XP_022990903.1 (defensin J1-2-like [Cucurbita maxima])

HSP 1 Score: 127.9 bits (320), Expect = 3.0e-26
Identity = 54/60 (90.00%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
          LG GMAPM  E RTCES SRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 15 LGTGMAPMGTEARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

[Close]

BLAST of MS018775.1 vs. NCBI nr
Match: XP_022922106.1 (defensin J1-2-like [Cucurbita moschata])

HSP 1 Score: 126.7 bits (317), Expect = 6.7e-26
Identity = 53/59 (89.83%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 2  GAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 61
          G+GMAPM  E RTCES SRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 17 GSGMAPMGTEARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 75

[Close]

BLAST of MS018775.1 vs. NCBI nr
Match: KAG6601912.1 (hypothetical protein SDJN03_07145, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 126.3 bits (316), Expect = 8.7e-26
Identity = 53/59 (89.83%), Postives = 54/59 (91.53%), Query Frame = 0


Query: 2  GAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 61
          G GMAPM  E RTCES SRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 17 GTGMAPMGTEARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 75

[Close]

BLAST of MS018775.1 vs. NCBI nr
Match: XP_023517597.1 (defensin J1-2-like [Cucurbita pepo subsp. pepo] >XP_023547787.1 defensin J1-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 125.9 bits (315), Expect = 1.1e-25
Identity = 52/60 (86.67%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
          LG GMAPM  E RTCES S HF+GLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 15 LGTGMAPMATEARTCESPSHHFRGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

[Close]

BLAST of MS018775.1 vs. ExPASy Swiss-Prot
Match: O65740 (Defensin J1-2 OS=Capsicum annuum OX=4072 PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.7e-22
Identity = 44/52 (84.62%), Postives = 47/52 (90.38%), Query Frame = 0


Query: 8  MVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          MVAE RTCESQS  FKGLCFSK+NCG VC TEGF+GGHCRGFRRRCFCT+HC
Sbjct: 23 MVAEARTCESQSHRFKGLCFSKSNCGSVCHTEGFNGGHCRGFRRRCFCTRHC 74

[Close]

BLAST of MS018775.1 vs. ExPASy Swiss-Prot
Match: Q39182 (Defensin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PDF2.2 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 1.1e-18
Identity = 38/59 (64.41%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          +  GM P+  E RTCESQS  FKG C S +NC +VC  EGF GG+CRGFRRRCFCT+HC
Sbjct: 19 VATGMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEGFVGGNCRGFRRRCFCTRHC 77

[Close]

BLAST of MS018775.1 vs. ExPASy Swiss-Prot
Match: Q9ZUL7 (Defensin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PDF2.3 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.2e-17
Identity = 36/59 (61.02%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          +  GM P+  E RTCES+S  FKG C S +NC +VC  EGF GG CRGFRRRC+CT+HC
Sbjct: 19 IATGMGPVTVEARTCESKSHRFKGPCVSTHNCANVCHNEGFGGGKCRGFRRRCYCTRHC 77

[Close]

BLAST of MS018775.1 vs. ExPASy Swiss-Prot
Match: Q41914 (Defensin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=PDF2.1 PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 1.0e-16
Identity = 36/59 (61.02%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          +  GM P+  E RTC SQS+ FKG C S  NC +VC  EGF GG CRGFRRRCFCT++C
Sbjct: 19 VATGMGPVTVEARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCRGFRRRCFCTRNC 77

[Close]

BLAST of MS018775.1 vs. ExPASy Swiss-Prot
Match: P82659 (Defensin SD2 OS=Helianthus annuus OX=4232 GN=SD2 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.3e-16
Identity = 36/53 (67.92%), Postives = 39/53 (73.58%), Query Frame = 0


Query: 7  PMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          P+V E RTCESQS  FKG C S  NC +VC +E F GG CRGFRRRCFCT HC
Sbjct: 26 PLVVEARTCESQSHKFKGTCLSDTNCANVCHSERFSGGKCRGFRRRCFCTTHC 78

[Close]

BLAST of MS018775.1 vs. ExPASy TrEMBL
Match: A0A6J1CXX5 (defensin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111015597 PE=4 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.2e-28
Identity = 58/60 (96.67%), Postives = 58/60 (96.67%), Query Frame = 0


Query: 1   LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
           LG GMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGG CRGFRRRCFCTKHCL
Sbjct: 87  LGTGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGRCRGFRRRCFCTKHCL 146

[Close]

BLAST of MS018775.1 vs. ExPASy TrEMBL
Match: A0A6J1JUM8 (defensin J1-2-like OS=Cucurbita maxima OX=3661 GN=LOC111487653 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-26
Identity = 54/60 (90.00%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
          LG GMAPM  E RTCES SRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 15 LGTGMAPMGTEARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

[Close]

BLAST of MS018775.1 vs. ExPASy TrEMBL
Match: A0A6J1E380 (defensin J1-2-like OS=Cucurbita moschata OX=3662 GN=LOC111430172 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.2e-26
Identity = 53/59 (89.83%), Postives = 55/59 (93.22%), Query Frame = 0


Query: 2  GAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 61
          G+GMAPM  E RTCES SRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 17 GSGMAPMGTEARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 75

[Close]

BLAST of MS018775.1 vs. ExPASy TrEMBL
Match: V5LEQ8 (Defensin-like protein 1 OS=Citrullus lanatus OX=3654 GN=PDF2.1 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.2e-26
Identity = 52/60 (86.67%), Postives = 54/60 (90.00%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
          L  GM PMV + RTCES SRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 15 LATGMGPMVTDARTCESPSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

[Close]

BLAST of MS018775.1 vs. ExPASy TrEMBL
Match: A0A0A0KJ50 (Knot1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G114570 PE=4 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-25
Identity = 52/60 (86.67%), Postives = 53/60 (88.33%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCL 60
          LG GM PMV E RTCES S  FKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC+
Sbjct: 15 LGTGMGPMVTEARTCESPSHRFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHCV 74

[Close]

BLAST of MS018775.1 vs. TAIR 10
Match: AT2G02100.1 (low-molecular-weight cysteine-rich 69 )

HSP 1 Score: 93.2 bits (230), Expect = 7.6e-20
Identity = 38/59 (64.41%), Postives = 44/59 (74.58%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          +  GM P+  E RTCESQS  FKG C S +NC +VC  EGF GG+CRGFRRRCFCT+HC
Sbjct: 19 VATGMGPVTVEARTCESQSHRFKGTCVSASNCANVCHNEGFVGGNCRGFRRRCFCTRHC 77

[Close]

BLAST of MS018775.1 vs. TAIR 10
Match: AT2G02130.1 (low-molecular-weight cysteine-rich 68 )

HSP 1 Score: 89.7 bits (221), Expect = 8.4e-19
Identity = 36/59 (61.02%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          +  GM P+  E RTCES+S  FKG C S +NC +VC  EGF GG CRGFRRRC+CT+HC
Sbjct: 19 IATGMGPVTVEARTCESKSHRFKGPCVSTHNCANVCHNEGFGGGKCRGFRRRCYCTRHC 77

[Close]

BLAST of MS018775.1 vs. TAIR 10
Match: AT2G02120.1 (Scorpion toxin-like knottin superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 7.1e-18
Identity = 36/59 (61.02%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          +  GM P+  E RTC SQS+ FKG C S  NC +VC  EGF GG CRGFRRRCFCT++C
Sbjct: 19 VATGMGPVTVEARTCASQSQRFKGKCVSDTNCENVCHNEGFPGGDCRGFRRRCFCTRNC 77

[Close]

BLAST of MS018775.1 vs. TAIR 10
Match: AT5G63660.1 (Scorpion toxin-like knottin superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 9.6e-15
Identity = 30/49 (61.22%), Postives = 37/49 (75.51%), Query Frame = 0


Query: 11 EGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          EGRTC+S+S HFK +C S +NC  VC+ EGF GG C GF RRC+CT+ C
Sbjct: 25 EGRTCQSKSHHFKYMCTSNHNCAIVCRNEGFSGGRCHGFHRRCYCTRLC 73

[Close]

BLAST of MS018775.1 vs. TAIR 10
Match: AT1G61070.1 (low-molecular-weight cysteine-rich 66 )

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-13
Identity = 31/59 (52.54%), Postives = 36/59 (61.02%), Query Frame = 0


Query: 1  LGAGMAPMVAEGRTCESQSRHFKGLCFSKNNCGHVCKTEGFHGGHCRGFRRRCFCTKHC 60
          L   M  +  E RTCE+ S  F G C S +NC +VC  EGF  G CRGFRRRC CT+ C
Sbjct: 18 LATVMGLVTVEARTCETSSNLFNGPCLSSSNCANVCHNEGFSDGDCRGFRRRCLCTRPC 76

The following BLAST results are available for this feature:

BLAST of MS018775.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146369.1	2.5e-28	96.67	defensin-like protein 2 [Momordica charantia]	[more]
XP_022990903.1	3.0e-26	90.00	defensin J1-2-like [Cucurbita maxima]	[more]
XP_022922106.1	6.7e-26	89.83	defensin J1-2-like [Cucurbita moschata]	[more]
KAG6601912.1	8.7e-26	89.83	hypothetical protein SDJN03_07145, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023517597.1	1.1e-25	86.67	defensin J1-2-like [Cucurbita pepo subsp. pepo] >XP_023547787.1 defensin J1-2-li...	[more]

BLAST of MS018775.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65740	2.7e-22	84.62	Defensin J1-2 OS=Capsicum annuum OX=4072 PE=1 SV=1	[more]
Q39182	1.1e-18	64.41	Defensin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=PDF2.2 PE=2 SV=1	[more]
Q9ZUL7	1.2e-17	61.02	Defensin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=PDF2.3 PE=2 SV=1	[more]
Q41914	1.0e-16	61.02	Defensin-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=PDF2.1 PE=2 SV=2	[more]
P82659	1.3e-16	67.92	Defensin SD2 OS=Helianthus annuus OX=4232 GN=SD2 PE=2 SV=1	[more]

BLAST of MS018775.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXX5	1.2e-28	96.67	defensin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111015597 PE=4 SV=1	[more]
A0A6J1JUM8	1.5e-26	90.00	defensin J1-2-like OS=Cucurbita maxima OX=3661 GN=LOC111487653 PE=4 SV=1	[more]
A0A6J1E380	3.2e-26	89.83	defensin J1-2-like OS=Cucurbita moschata OX=3662 GN=LOC111430172 PE=4 SV=1	[more]
V5LEQ8	7.2e-26	86.67	Defensin-like protein 1 OS=Citrullus lanatus OX=3654 GN=PDF2.1 PE=2 SV=1	[more]
A0A0A0KJ50	2.1e-25	86.67	Knot1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G114570 PE=4 ...	[more]

BLAST of MS018775.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02100.1	7.6e-20	64.41	low-molecular-weight cysteine-rich 69	[more]
AT2G02130.1	8.4e-19	61.02	low-molecular-weight cysteine-rich 68	[more]
AT2G02120.1	7.1e-18	61.02	Scorpion toxin-like knottin superfamily protein	[more]
AT5G63660.1	9.6e-15	61.22	Scorpion toxin-like knottin superfamily protein	[more]
AT1G61070.1	1.8e-13	52.54	low-molecular-weight cysteine-rich 66	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008176	Defensin, plant	PRINTS	PR00288	PUROTHIONIN	coord: 14..29 score: 53.8 coord: 44..58 score: 61.45
IPR008176	Defensin, plant	PROSITE	PS00940	GAMMA_THIONIN	coord: 13..36
IPR003614	Knottin, scorpion toxin-like	SMART	SM00505	gth_4	coord: 14..59 e-value: 7.7E-12 score: 55.3
IPR003614	Knottin, scorpion toxin-like	CDD	cd00107	Knot1	coord: 24..56 e-value: 2.10462E-5 score: 35.0184
None	No IPR available	PFAM	PF00304	Gamma-thionin	coord: 13..59 e-value: 2.0E-17 score: 63.2
None	No IPR available	PANTHER	PTHR33147	DEFENSIN-LIKE PROTEIN 1	coord: 8..59
None	No IPR available	PANTHER	PTHR33147:SF69	DEFENSIN SD2	coord: 8..59
IPR036574	Knottin, scorpion toxin-like superfamily	GENE3D	3.30.30.10		coord: 13..59 e-value: 8.2E-21 score: 75.9
IPR036574	Knottin, scorpion toxin-like superfamily	SUPERFAMILY	57095	Scorpion toxin-like	coord: 13..59

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS018775	MS018775	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS018775.1-cds	MS018775.1-cds-scaffold313:1880966..1881145	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS018775.1	MS018775.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response