MS017018.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS017018.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold197: 672117 .. 673034 (+)
Sequence length	918
RNA-Seq Expression	MS017018.1
Synteny	MS017018.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAGTGGGCGGAACACACATTGGTGTTATAGATGCAGAAGGTCGGTACATCTTCGTGGTCGAGATGCAGTTTGCCCCAACTGTAATGGAGGATTTATCCAAGAACTTGATGATATGGTGCCTAGCAACCCATTTGATTTCTTTGGACTGGATCATGATGAAGATCGCGGTCAAGGGCTGGGAGCATTCTCCGCTTTTATGAGACAACGGTTAGCAGAAAGAAACGACATGAGGGGAAGATCGGAGTCAGTTTTTGAGCGTAGCCCGGGATTCGGTCCTCTGCTAATATTTGGTGGTCAAATTCCATTGAGAAACAGCAGGTTAGAAGCCCTCTTTAGTGGGGCTCCTGGCATTGGTATTACACGGGGCGATAGCGGCGACTACTTCATAGGCCCTGGACTGGAGGAGCTATTCGAACAGCTCTCAGTAAATGGTAATCGTGGCCCTCCACCAGCATCTAGATCATCAATTGACGCAATGCCAACAGTTAAGATTACGCAACGACATCTTCGGTCAGACTCGCATTGTCCGGTTTGCAAAGACAAATTCGAACTCGGGTCTGAAGCTAGGCAGATGCCGTGTAATCACATGTACCACTCAGATTGTATCATTCCATGGTTGGTTCAGCACAACTCTTGCCCTGTTTGCCGCCAGGAACTGCCACCGCAGGGAATAGGTGGCGGAGGCGGGGGTGGGGGAGGGGGCCATAGCACCAACGGTCAAAACCGAAGTAGCAGCAGCTACAACAACAACAGCAATGGCTCCAGGGTTAATCCAGGAAGACGGAATCCATTTTCTTATCTGTGGCCATTTCGGTCGTCTAACTCTAGGTCCAACCATAGCGGAGGCGGAGGCGGAGCCGGGAGCAGCGAACCGATGAACCACCAGATGGAGTATCCGGGATGGCAATTTACT

mRNA sequence

ATGTCGAGTGGGCGGAACACACATTGGTGTTATAGATGCAGAAGGTCGGTACATCTTCGTGGTCGAGATGCAGTTTGCCCCAACTGTAATGGAGGATTTATCCAAGAACTTGATGATATGGTGCCTAGCAACCCATTTGATTTCTTTGGACTGGATCATGATGAAGATCGCGGTCAAGGGCTGGGAGCATTCTCCGCTTTTATGAGACAACGGTTAGCAGAAAGAAACGACATGAGGGGAAGATCGGAGTCAGTTTTTGAGCGTAGCCCGGGATTCGGTCCTCTGCTAATATTTGGTGGTCAAATTCCATTGAGAAACAGCAGGTTAGAAGCCCTCTTTAGTGGGGCTCCTGGCATTGGTATTACACGGGGCGATAGCGGCGACTACTTCATAGGCCCTGGACTGGAGGAGCTATTCGAACAGCTCTCAGTAAATGGTAATCGTGGCCCTCCACCAGCATCTAGATCATCAATTGACGCAATGCCAACAGTTAAGATTACGCAACGACATCTTCGGTCAGACTCGCATTGTCCGGTTTGCAAAGACAAATTCGAACTCGGGTCTGAAGCTAGGCAGATGCCGTGTAATCACATGTACCACTCAGATTGTATCATTCCATGGTTGGTTCAGCACAACTCTTGCCCTGTTTGCCGCCAGGAACTGCCACCGCAGGGAATAGGTGGCGGAGGCGGGGGTGGGGGAGGGGGCCATAGCACCAACGGTCAAAACCGAAGTAGCAGCAGCTACAACAACAACAGCAATGGCTCCAGGGTTAATCCAGGAAGACGGAATCCATTTTCTTATCTGTGGCCATTTCGGTCGTCTAACTCTAGGTCCAACCATAGCGGAGGCGGAGGCGGAGCCGGGAGCAGCGAACCGATGAACCACCAGATGGAGTATCCGGGATGGCAATTTACT

Coding sequence (CDS)

ATGTCGAGTGGGCGGAACACACATTGGTGTTATAGATGCAGAAGGTCGGTACATCTTCGTGGTCGAGATGCAGTTTGCCCCAACTGTAATGGAGGATTTATCCAAGAACTTGATGATATGGTGCCTAGCAACCCATTTGATTTCTTTGGACTGGATCATGATGAAGATCGCGGTCAAGGGCTGGGAGCATTCTCCGCTTTTATGAGACAACGGTTAGCAGAAAGAAACGACATGAGGGGAAGATCGGAGTCAGTTTTTGAGCGTAGCCCGGGATTCGGTCCTCTGCTAATATTTGGTGGTCAAATTCCATTGAGAAACAGCAGGTTAGAAGCCCTCTTTAGTGGGGCTCCTGGCATTGGTATTACACGGGGCGATAGCGGCGACTACTTCATAGGCCCTGGACTGGAGGAGCTATTCGAACAGCTCTCAGTAAATGGTAATCGTGGCCCTCCACCAGCATCTAGATCATCAATTGACGCAATGCCAACAGTTAAGATTACGCAACGACATCTTCGGTCAGACTCGCATTGTCCGGTTTGCAAAGACAAATTCGAACTCGGGTCTGAAGCTAGGCAGATGCCGTGTAATCACATGTACCACTCAGATTGTATCATTCCATGGTTGGTTCAGCACAACTCTTGCCCTGTTTGCCGCCAGGAACTGCCACCGCAGGGAATAGGTGGCGGAGGCGGGGGTGGGGGAGGGGGCCATAGCACCAACGGTCAAAACCGAAGTAGCAGCAGCTACAACAACAACAGCAATGGCTCCAGGGTTAATCCAGGAAGACGGAATCCATTTTCTTATCTGTGGCCATTTCGGTCGTCTAACTCTAGGTCCAACCATAGCGGAGGCGGAGGCGGAGCCGGGAGCAGCGAACCGATGAACCACCAGATGGAGTATCCGGGATGGCAATTTACT

Protein sequence

MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQGLGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIGITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEYPGWQFT

Homology

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BLAST of MS017018.1 vs. NCBI nr
Match: XP_022154478.1 (probable E3 ubiquitin-protein ligase RHC1A [Momordica charantia])

HSP 1 Score: 640.2 bits (1650), Expect = 9.2e-180
Identity = 305/306 (99.67%), Postives = 305/306 (99.67%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG
Sbjct: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGG GGGHSTN
Sbjct: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGAGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
           GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY
Sbjct: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300

Query: 301 PGWQFT 307
           PGWQFT
Sbjct: 301 PGWQFT 306

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BLAST of MS017018.1 vs. NCBI nr
Match: XP_038882645.1 (probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882646.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882647.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882648.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882649.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882650.1 probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida])

HSP 1 Score: 558.1 bits (1437), Expect = 4.6e-155
Identity = 270/310 (87.10%), Postives = 284/310 (91.61%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MS+GRNTHWCYRCRRSV L GRDAVCP+CNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSTGRNTHWCYRCRRSVRLHGRDAVCPSCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRL ERNDMRGRSES+FERSPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLVERNDMRGRSESLFERSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTV ITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSANGHRGPPPASRSSIDAMPTVNITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGI----GGGGGGGGGG 240
           K+KFELG EARQMPCNHMYHSDCI+PWLVQHNSCPVCRQELPPQGI    GGGGGGGGGG
Sbjct: 181 KEKFELGCEARQMPCNHMYHSDCIVPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGGGGG 240

Query: 241 HSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNH 300
           H+TNGQNRS+S    N +GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NH
Sbjct: 241 HNTNGQNRSNS---YNGSGSRVNPGRRNPFSYLWPFRSSSSSSNH----GGAGSSEPTNH 300

Query: 301 QMEYPGWQFT 307
           QM Y G+++T
Sbjct: 301 QMGYSGYRYT 303

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BLAST of MS017018.1 vs. NCBI nr
Match: XP_004144313.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_004144314.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_011653845.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_031739891.1 probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >KGN54669.1 hypothetical protein Csa_012618 [Cucumis sativus])

HSP 1 Score: 557.0 bits (1434), Expect = 1.0e-154
Identity = 271/306 (88.56%), Postives = 283/306 (92.48%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MS+GRNTHWCYRCRRSV LRGRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWL+QHNSCPVCRQELPPQGI    GGGGGGHSTN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGI----GGGGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
            QNR S+SYNN S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 DQNR-SNSYNNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 296

Query: 301 PGWQFT 307
            G+++T
Sbjct: 301 SGYRYT 296

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BLAST of MS017018.1 vs. NCBI nr
Match: XP_008455755.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455757.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0025786.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK09669.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 546.2 bits (1406), Expect = 1.8e-151
Identity = 266/306 (86.93%), Postives = 280/306 (91.50%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MS+GRNTHWCYRCRRSV L GRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LS NG+RGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWL+QHNSCPVCRQELP QGI    GGGGGGHSTN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGI----GGGGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
            QNR S+SY+N S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 DQNR-SNSYSNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 296

Query: 301 PGWQFT 307
            G+++T
Sbjct: 301 SGYRYT 296

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BLAST of MS017018.1 vs. NCBI nr
Match: XP_023517925.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_023517926.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 531.9 bits (1369), Expect = 3.5e-147
Identity = 260/303 (85.81%), Postives = 272/303 (89.77%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSSG+NTHWCYRCRRS+ L GRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSSGQNTHWCYRCRRSIRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FER+PGFGPLLIFGGQIPLRN RLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFERNPGFGPLLIFGGQIPLRNRRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGD GDYFIGPGLEELFEQLS NG+RGPPPASRS IDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDGGDYFIGPGLEELFEQLSENGHRGPPPASRSLIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWLVQHNSCPVCRQELPPQ +      GGGGH+TN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQVM------GGGGHNTN 240

Query: 241 GQNRSSSSYNNNSNG-SRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQME 300
            QNR ++SYNNN NG SRVNPGRRNPFSYLWPFRSSNS SNH    GGAGSSE  NH M 
Sbjct: 241 SQNR-NNSYNNNGNGSSRVNPGRRNPFSYLWPFRSSNSSSNH----GGAGSSESRNHPMG 292

Query: 301 YPG 303
           Y G
Sbjct: 301 YSG 292

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BLAST of MS017018.1 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 8.5e-88
Identity = 184/331 (55.59%), Postives = 225/331 (67.98%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFF----GLDHDED 60
           MSS RNTHWC+RC+R+V L G++ VC  C GGF++EL DM  ++PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RGQGLGAFSAFMRQRLAERN---DMRGRS-ESVFERSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFS-GAPGIGITRGDSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKIT 180
           EALF+ G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCKD+FELGSEA+QMPCNH+YHSDCI+PWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP---- 240

Query: 241 GGGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGG 300
              G       +T  +N  SSS +++SN       RRNPFS  WPFRSS S S+ +   G
Sbjct: 241 SASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRG 300

Query: 301 GAGSSE---------PMNHQ---MEYPGWQF 306
           G  +S+            HQ   M Y GW F
Sbjct: 301 GTRNSDTSDENHNYHQQQHQQSYMGYSGWPF 326

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BLAST of MS017018.1 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.6e-79
Identity = 167/295 (56.61%), Postives = 199/295 (67.46%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSS RNTHWC+RC+R+V LR RDAVC  C GGF++E+ D+ PS        D   D    
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRDVERDPTFDL--- 60

Query: 61  LGAFSAFMRQRLAERN---DMRGRSESVFERS-PGFGPLLIFGGQIPLR-----NSRLEA 120
           + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS +EA
Sbjct: 61  MEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEA 120

Query: 121 LFSG-APGIGITRG-DSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKITQ 180
             +G APGIGI RG ++GDYF GPGLEEL EQLS    +RGPPPA +SSIDA+PT+KITQ
Sbjct: 121 FVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKITQ 180

Query: 181 RHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIG 240
           +HL+ SDSHCPVCKD+FEL SEA+QMPC+H+YHSDCI+PWLVQHNSCPVCR+ELP     
Sbjct: 181 KHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELP----- 240

Query: 241 GGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHS 283
                      + G + S+ S  N S   R N  RRN FS LWPFRSS+S S  +
Sbjct: 241 -----------SRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPFRSSSSSSTQN 275

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BLAST of MS017018.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 9.9e-36
Identity = 109/320 (34.06%), Postives = 152/320 (47.50%), Query Frame = 0


Query: 9   WCYRCRRSVHL---RGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDE---------- 68
           +CY+C ++V +      D  CP CN GF++E +D  P+   +F     D           
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFST 81

Query: 69  ----DRGQGLGAFSAF-MRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQI-PLRNS-- 128
                 G    A S              + RS     +S  F P       +  LR+S  
Sbjct: 82  LLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTLRSSGT 141

Query: 129 RLEALFSGAPGIGITR--GDSGDYFIGPGLEELFEQLSVNG-NR-GPPPASRSSIDAMPT 188
             E +    P     R  G+ GDYF GPGLE+L +QL+ N  NR G PPAS+S+IDA+PT
Sbjct: 142 HFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPT 201

Query: 189 VKITQRHLRSD-SHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELP 248
           VK+T+  L+S+ + C VC D+FE GS+ +QMPC H++H DC++PWL  HNSCPVCR ELP
Sbjct: 202 VKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

Query: 249 PQGIGGGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYL--WPFRSSNSRSN 300
                            + +NRS  S  +      V   +   FS    WPFR  +   +
Sbjct: 262 TD-------------DPDYENRSQGSQGSGDGQGSVEGQQTPRFSIQLPWPFRRQDGSGS 321

[Close]

BLAST of MS017018.1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.4e-35
Identity = 100/281 (35.59%), Postives = 143/281 (50.89%), Query Frame = 0


Query: 9   WCYRCRRSVHLR----GRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQGL--- 68
           +CY+C R+V++       D  CP CN GF++E D+  P+    F   + +      L   
Sbjct: 24  FCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLT 83

Query: 69  --GAFSAFMRQRL-AERNDMRGRSESVFERSP--------------GFGPLLIFGGQI-P 128
               F++ +     +  +     S S+   +P               F P       +  
Sbjct: 84  LSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLND 143

Query: 129 LRNS--RLEALFSGAPGIGITR--GDSGDYFIGPGLEELFEQLSVNG-NR-GPPPASRSS 188
           LR+S  ++E +    P     R   + GDYFIGPGLE+L +QL+ N  NR G PPAS+S+
Sbjct: 144 LRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSA 203

Query: 189 IDAMPTVKITQRHLRSD-SHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPV 246
           I+A+P V IT+ +L S+ + C VC D FE G+EA+QMPC H+YH DC++PWL  HNSCPV
Sbjct: 204 IEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPV 263

[Close]

BLAST of MS017018.1 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-31
Identity = 94/263 (35.74%), Postives = 135/263 (51.33%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAV-CPNCNGGFIQELDDMVPSNPFDFFGLDHDEDR-- 60
           M+SG  ++WCY C R V +   D++ CP+C+GGF++ + + +   P D F          
Sbjct: 1   MASG--SYWCYSCSRFVWV--SDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRS 60

Query: 61  -------GQGLGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRL- 120
                         SA M    +    +  R+ S   RSP   P+++  G     +S + 
Sbjct: 61  PTRFPPPSSSSSTPSASMHADNSPTPTIVTRTRS--NRSP--NPVIVLRGSAAAPSSDVV 120

Query: 121 -EALFSGAPGIGITRG-DSG---------DYFIGPGLEELFEQLS---VNGNRG-----P 180
            E L   A  +    G DSG         ++ +G G + L +Q+S   +N NR       
Sbjct: 121 SEGLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEH 180

Query: 181 PPASRSSIDAMPTVKITQRHLRSD--SHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWL 232
           PPAS+S+I+A+P ++I   HL SD  SHC VCK+ F L S AR+MPCNH+YH DCI+PWL
Sbjct: 181 PPASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWL 240

[Close]

BLAST of MS017018.1 vs. ExPASy TrEMBL
Match: A0A6J1DKF0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021749 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 4.4e-180
Identity = 305/306 (99.67%), Postives = 305/306 (99.67%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG
Sbjct: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGG GGGHSTN
Sbjct: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGAGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
           GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY
Sbjct: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300

Query: 301 PGWQFT 307
           PGWQFT
Sbjct: 301 PGWQFT 306

[Close]

BLAST of MS017018.1 vs. ExPASy TrEMBL
Match: A0A0A0L0T9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 4.9e-155
Identity = 271/306 (88.56%), Postives = 283/306 (92.48%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MS+GRNTHWCYRCRRSV LRGRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSAGRNTHWCYRCRRSVRLRGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFEQLS NG+RGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFEQLSENGHRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWL+QHNSCPVCRQELPPQGI    GGGGGGHSTN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPPQGI----GGGGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
            QNR S+SYNN S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 DQNR-SNSYNNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 296

Query: 301 PGWQFT 307
            G+++T
Sbjct: 301 SGYRYT 296

[Close]

BLAST of MS017018.1 vs. ExPASy TrEMBL
Match: A0A1S3C178 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 8.7e-152
Identity = 266/306 (86.93%), Postives = 280/306 (91.50%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MS+GRNTHWCYRCRRSV L GRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LS NG+RGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWL+QHNSCPVCRQELP QGI    GGGGGGHSTN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGI----GGGGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
            QNR S+SY+N S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 DQNR-SNSYSNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 296

Query: 301 PGWQFT 307
            G+++T
Sbjct: 301 SGYRYT 296

[Close]

BLAST of MS017018.1 vs. ExPASy TrEMBL
Match: A0A5A7SIY2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00710 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 8.7e-152
Identity = 266/306 (86.93%), Postives = 280/306 (91.50%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MS+GRNTHWCYRCRRSV L GRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSAGRNTHWCYRCRRSVRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGRSES+FE SPGFGPLLIFGGQIPLRNSRLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRSESLFEHSPGFGPLLIFGGQIPLRNSRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGDSGDYFIGPGLEELFE+LS NG+RGPPPASRSSIDAMPTVKIT RHLRSDSHCPVC
Sbjct: 121 ITRGDSGDYFIGPGLEELFERLSANGHRGPPPASRSSIDAMPTVKITHRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWL+QHNSCPVCRQELP QGI    GGGGGGHSTN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLIQHNSCPVCRQELPSQGI----GGGGGGHSTN 240

Query: 241 GQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQMEY 300
            QNR S+SY+N S GSRVNPGRRNPFSYLWPFRSS+S SNH    GGAGSSEP NHQM Y
Sbjct: 241 DQNR-SNSYSNGS-GSRVNPGRRNPFSYLWPFRSSSSNSNH----GGAGSSEPSNHQMGY 296

Query: 301 PGWQFT 307
            G+++T
Sbjct: 301 SGYRYT 296

[Close]

BLAST of MS017018.1 vs. ExPASy TrEMBL
Match: A0A6J1EAP6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432344 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.8e-147
Identity = 259/303 (85.48%), Postives = 272/303 (89.77%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSSG+NTHWCYRCRRS+ L GRDAVCPNCNGGFIQELDDMVP NPFDFFGLDHDEDR  G
Sbjct: 1   MSSGQNTHWCYRCRRSIRLCGRDAVCPNCNGGFIQELDDMVPGNPFDFFGLDHDEDRDHG 60

Query: 61  LGAFSAFMRQRLAERNDMRGRSESVFERSPGFGPLLIFGGQIPLRNSRLEALFSGAPGIG 120
           LGAFSAFMRQRLAERNDMRGR+ES+FER+PGFGPLLIFGGQIPLRN RLEALF+GAPGIG
Sbjct: 61  LGAFSAFMRQRLAERNDMRGRAESLFERNPGFGPLLIFGGQIPLRNRRLEALFNGAPGIG 120

Query: 121 ITRGDSGDYFIGPGLEELFEQLSVNGNRGPPPASRSSIDAMPTVKITQRHLRSDSHCPVC 180
           ITRGD GDYFIGPGLEELFEQLS NG+RGPPPASRS IDAMPTVKITQRHLRSDSHCPVC
Sbjct: 121 ITRGDGGDYFIGPGLEELFEQLSENGHRGPPPASRSLIDAMPTVKITQRHLRSDSHCPVC 180

Query: 181 KDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIGGGGGGGGGGHSTN 240
           K+KFELGSEARQM CNHMYHSDCI+PWLVQHNSCPVCRQELPPQ +      GG GH+TN
Sbjct: 181 KEKFELGSEARQMACNHMYHSDCIVPWLVQHNSCPVCRQELPPQVM------GGAGHNTN 240

Query: 241 GQNRSSSSYNNNSNG-SRVNPGRRNPFSYLWPFRSSNSRSNHSGGGGGAGSSEPMNHQME 300
            QNR ++SYNNN NG SRVNPGRRNPFSYLWPFRSSNS SNH    GGAGSSE  NHQM 
Sbjct: 241 SQNR-NNSYNNNGNGSSRVNPGRRNPFSYLWPFRSSNSSSNH----GGAGSSESRNHQMG 292

Query: 301 YPG 303
           Y G
Sbjct: 301 YSG 292

[Close]

BLAST of MS017018.1 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 325.1 bits (832), Expect = 6.1e-89
Identity = 184/331 (55.59%), Postives = 225/331 (67.98%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFF----GLDHDED 60
           MSS RNTHWC+RC+R+V L G++ VC  C GGF++EL DM  ++PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RGQGLGAFSAFMRQRLAERN---DMRGRS-ESVFERSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFS-GAPGIGITRGDSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKIT 180
           EALF+ G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCKD+FELGSEA+QMPCNH+YHSDCI+PWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP---- 240

Query: 241 GGGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGG 300
              G       +T  +N  SSS +++SN       RRNPFS  WPFRSS S S+ +   G
Sbjct: 241 SASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRG 300

Query: 301 GAGSSE---------PMNHQ---MEYPGWQF 306
           G  +S+            HQ   M Y GW F
Sbjct: 301 GTRNSDTSDENHNYHQQQHQQSYMGYSGWPF 326

[Close]

BLAST of MS017018.1 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 325.1 bits (832), Expect = 6.1e-89
Identity = 184/331 (55.59%), Postives = 225/331 (67.98%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFF----GLDHDED 60
           MSS RNTHWC+RC+R+V L G++ VC  C GGF++EL DM  ++PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RGQGLGAFSAFMRQRLAERN---DMRGRS-ESVFERSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFS-GAPGIGITRGDSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKIT 180
           EALF+ G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCKD+FELGSEA+QMPCNH+YHSDCI+PWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP---- 240

Query: 241 GGGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGG 300
              G       +T  +N  SSS +++SN       RRNPFS  WPFRSS S S+ +   G
Sbjct: 241 SASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRG 300

Query: 301 GAGSSE---------PMNHQ---MEYPGWQF 306
           G  +S+            HQ   M Y GW F
Sbjct: 301 GTRNSDTSDENHNYHQQQHQQSYMGYSGWPF 326

[Close]

BLAST of MS017018.1 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 325.1 bits (832), Expect = 6.1e-89
Identity = 184/331 (55.59%), Postives = 225/331 (67.98%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFF----GLDHDED 60
           MSS RNTHWC+RC+R+V L G++ VC  C GGF++EL DM  ++PFD F    G+   + 
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL-DMAQASPFDMFRSHRGVVERDQ 60

Query: 61  RGQGLGAFSAFMRQRLAERN---DMRGRS-ESVFERSPGFGPLLIFGGQIPLR---NSRL 120
               + AFS FMR RLAER+   ++RGR+  S  E  PG  PLLIFGGQ+P R   ++ +
Sbjct: 61  TFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAV 120

Query: 121 EALFS-GAPGIGITRGDSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKIT 180
           EALF+ G+PGIGITRG++GDYF GPGLEELFEQLS     RGPPPA RS+IDA+PT+KI 
Sbjct: 121 EALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIA 180

Query: 181 QRHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGI 240
           QRHLR SDS+CPVCKD+FELGSEA+QMPCNH+YHSDCI+PWLVQHNSCPVCRQELP    
Sbjct: 181 QRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELP---- 240

Query: 241 GGGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHSGGGG 300
              G       +T  +N  SSS +++SN       RRNPFS  WPFRSS S S+ +   G
Sbjct: 241 SASGPSSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRG 300

Query: 301 GAGSSE---------PMNHQ---MEYPGWQF 306
           G  +S+            HQ   M Y GW F
Sbjct: 301 GTRNSDTSDENHNYHQQQHQQSYMGYSGWPF 326

[Close]

BLAST of MS017018.1 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.9e-80
Identity = 167/295 (56.61%), Postives = 199/295 (67.46%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSS RNTHWC+RC+R+V LR RDAVC  C GGF++E+ D+ PS        D   D    
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRDVERDPTFDL--- 60

Query: 61  LGAFSAFMRQRLAERN---DMRGRSESVFERS-PGFGPLLIFGGQIPLR-----NSRLEA 120
           + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS +EA
Sbjct: 61  MEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEA 120

Query: 121 LFSG-APGIGITRG-DSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKITQ 180
             +G APGIGI RG ++GDYF GPGLEEL EQLS    +RGPPPA +SSIDA+PT+KITQ
Sbjct: 121 FVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKITQ 180

Query: 181 RHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIG 240
           +HL+ SDSHCPVCKD+FEL SEA+QMPC+H+YHSDCI+PWLVQHNSCPVCR+ELP     
Sbjct: 181 KHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELP----- 240

Query: 241 GGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHS 283
                      + G + S+ S  N S   R N  RRN FS LWPFRSS+S S  +
Sbjct: 241 -----------SRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPFRSSSSSSTQN 275

[Close]

BLAST of MS017018.1 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 3.9e-80
Identity = 167/295 (56.61%), Postives = 199/295 (67.46%), Query Frame = 0


Query: 1   MSSGRNTHWCYRCRRSVHLRGRDAVCPNCNGGFIQELDDMVPSNPFDFFGLDHDEDRGQG 60
           MSS RNTHWC+RC+R+V LR RDAVC  C GGF++E+ D+ PS        D   D    
Sbjct: 1   MSSIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEI-DIGPSRAHRDVERDPTFDL--- 60

Query: 61  LGAFSAFMRQRLAERN---DMRGRSESVFERS-PGFGPLLIFGGQIPLR-----NSRLEA 120
           + AFSAFMR RLAER+   ++ GR  S    S     PLLIFGGQ P R     NS +EA
Sbjct: 61  MEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVEA 120

Query: 121 LFSG-APGIGITRG-DSGDYFIGPGLEELFEQLSV-NGNRGPPPASRSSIDAMPTVKITQ 180
             +G APGIGI RG ++GDYF GPGLEEL EQLS    +RGPPPA +SSIDA+PT+KITQ
Sbjct: 121 FVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKITQ 180

Query: 181 RHLR-SDSHCPVCKDKFELGSEARQMPCNHMYHSDCIIPWLVQHNSCPVCRQELPPQGIG 240
           +HL+ SDSHCPVCKD+FEL SEA+QMPC+H+YHSDCI+PWLVQHNSCPVCR+ELP     
Sbjct: 181 KHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELP----- 240

Query: 241 GGGGGGGGGHSTNGQNRSSSSYNNNSNGSRVNPGRRNPFSYLWPFRSSNSRSNHS 283
                      + G + S+ S  N S   R N  RRN FS LWPFRSS+S S  +
Sbjct: 241 -----------SRGSSSSTQSSQNRSTNGRENSRRRNIFSNLWPFRSSSSSSTQN 275

The following BLAST results are available for this feature:

BLAST of MS017018.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154478.1	9.2e-180	99.67	probable E3 ubiquitin-protein ligase RHC1A [Momordica charantia]	[more]
XP_038882645.1	4.6e-155	87.10	probable E3 ubiquitin-protein ligase RHC1A [Benincasa hispida] >XP_038882646.1 p...	[more]
XP_004144313.1	1.0e-154	88.56	probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >XP_004144314.1 pro...	[more]
XP_008455755.1	1.8e-151	86.93	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008455756.1...	[more]
XP_023517925.1	3.5e-147	85.81	probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo] >XP_0235...	[more]

BLAST of MS017018.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	8.5e-88	55.59	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	5.6e-79	56.61	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q8LPN7	9.9e-36	34.06	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	6.4e-35	35.59	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22283	1.9e-31	35.74	Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]

BLAST of MS017018.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKF0	4.4e-180	99.67	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102174...	[more]
A0A0A0L0T9	4.9e-155	88.56	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G419610 PE...	[more]
A0A1S3C178	8.7e-152	86.93	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495857 PE=4 ...	[more]
A0A5A7SIY2	8.7e-152	86.93	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1EAP6	3.8e-147	85.48	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432344...	[more]

BLAST of MS017018.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40830.2	6.1e-89	55.59	RING-H2 finger C1A	[more]
AT2G40830.1	6.1e-89	55.59	RING-H2 finger C1A	[more]
AT2G40830.3	6.1e-89	55.59	RING-H2 finger C1A	[more]
AT3G56580.2	3.9e-80	56.61	RING/U-box superfamily protein	[more]
AT3G56580.1	3.9e-80	56.61	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..217 e-value: 1.3E-7 score: 41.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..218 e-value: 3.5E-11 score: 43.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 177..218 score: 12.179465
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 140..224 e-value: 1.9E-22 score: 80.7
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 6..37 e-value: 7.2E-12 score: 45.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 236..261
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 225..306
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 269..286
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 2..294
None	No IPR available	PANTHER	PTHR15710:SF86	E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATED	coord: 2..294
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 177..218 e-value: 2.7861E-24 score: 90.84
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 157..224

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS017018	MS017018	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS017018.1-cds	MS017018.1-cds-scaffold197:672117..673034	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS017018.1	MS017018.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0061630	ubiquitin protein ligase activity