MS016567.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS016567.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	calcium-binding protein CML42
Location	scaffold1294: 128174 .. 128779 (-)
Sequence length	606
RNA-Seq Expression	MS016567.1
Synteny	MS016567.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCGGCGGCCTCAGATAACGGTAATGCTCCCCGACCCCAACCCCGGCTGACGAAGAAGCCCTCGTCTTCGTCGTTCCAGCTTCGGTGCCCCAGCCTGAATTCCCTACGTCTGCGTCGCATATTTGACCTCTTCGACAAGAACGGGGACGGGATGATCACGACCGAGGAGCTGGGTCAGGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGATCTGGATTCGACGGTCAACTCCTTCATCCAACCGGGAAACGACGGCCTGAGGTTCGAAGACTTTGCAGCCCTGCACCAGTCCTTAAACGACACCTATTTCGGGGGGTCGGAGGAGGGCGAGGGCGAGGGTGAGGAGCGGGAAAGCATGTCGATGTCGCAGGAGGAGGCGGATCTAACGGAGGCCTTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCGCCGAGCTGCAAGTGGTTCTAAGAAAGCTGGGATTGTCGGAGGGGAACGAGATCGACAGGGTTCAGAAGATGATCGTCTCCGTTGACCGCAATCAAGACGGCCGGGTTGATTTCTTCGAGTTCAAGGACATGATGCGCAGTGTAATCGTCAGGAGTTGT

mRNA sequence

ATGGAAGCGGCGGCCTCAGATAACGGTAATGCTCCCCGACCCCAACCCCGGCTGACGAAGAAGCCCTCGTCTTCGTCGTTCCAGCTTCGGTGCCCCAGCCTGAATTCCCTACGTCTGCGTCGCATATTTGACCTCTTCGACAAGAACGGGGACGGGATGATCACGACCGAGGAGCTGGGTCAGGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGATCTGGATTCGACGGTCAACTCCTTCATCCAACCGGGAAACGACGGCCTGAGGTTCGAAGACTTTGCAGCCCTGCACCAGTCCTTAAACGACACCTATTTCGGGGGGTCGGAGGAGGGCGAGGGCGAGGGTGAGGAGCGGGAAAGCATGTCGATGTCGCAGGAGGAGGCGGATCTAACGGAGGCCTTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCGCCGAGCTGCAAGTGGTTCTAAGAAAGCTGGGATTGTCGGAGGGGAACGAGATCGACAGGGTTCAGAAGATGATCGTCTCCGTTGACCGCAATCAAGACGGCCGGGTTGATTTCTTCGAGTTCAAGGACATGATGCGCAGTGTAATCGTCAGGAGTTGT

Coding sequence (CDS)

ATGGAAGCGGCGGCCTCAGATAACGGTAATGCTCCCCGACCCCAACCCCGGCTGACGAAGAAGCCCTCGTCTTCGTCGTTCCAGCTTCGGTGCCCCAGCCTGAATTCCCTACGTCTGCGTCGCATATTTGACCTCTTCGACAAGAACGGGGACGGGATGATCACGACCGAGGAGCTGGGTCAGGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGATCTGGATTCGACGGTCAACTCCTTCATCCAACCGGGAAACGACGGCCTGAGGTTCGAAGACTTTGCAGCCCTGCACCAGTCCTTAAACGACACCTATTTCGGGGGGTCGGAGGAGGGCGAGGGCGAGGGTGAGGAGCGGGAAAGCATGTCGATGTCGCAGGAGGAGGCGGATCTAACGGAGGCCTTCAGGGTATTCGACGAGGATGGAGATGGCTACATATCCGCCGCCGAGCTGCAAGTGGTTCTAAGAAAGCTGGGATTGTCGGAGGGGAACGAGATCGACAGGGTTCAGAAGATGATCGTCTCCGTTGACCGCAATCAAGACGGCCGGGTTGATTTCTTCGAGTTCAAGGACATGATGCGCAGTGTAATCGTCAGGAGTTGT

Protein sequence

MEAAASDNGNAPRPQPRLTKKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMRSVIVRSC

Homology

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BLAST of MS016567.1 vs. NCBI nr
Match: XP_022157322.1 (calcium-binding protein CML42 [Momordica charantia])

HSP 1 Score: 391.0 bits (1003), Expect = 6.4e-105
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG 60
           MEAAASDNGNAPRPQPRLTK PSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG
Sbjct: 1   MEAAASDNGNAPRPQPRLTKNPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG 60

Query: 61  QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEER 120
           QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEER
Sbjct: 61  QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEER 120

Query: 121 ESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRN 180
           ESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRN
Sbjct: 121 ESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 203
           QDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 202

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BLAST of MS016567.1 vs. NCBI nr
Match: XP_004137668.1 (calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein Csa_002212 [Cucumis sativus])

HSP 1 Score: 325.1 bits (832), Expect = 4.3e-85
Identity = 174/206 (84.47%), Postives = 187/206 (90.78%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPS--SSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKPS  SSSF+LRCPSLNSLRLRRIFD+FDKNGDGMITT+E
Sbjct: 1   MEAVASD-----VPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADES+LDSTVNSFI+PGN+GLRFEDFAALHQSLNDTYFGG  E E E E
Sbjct: 61  LGQALGLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDE 120

Query: 121 ERES--MSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVS 180
           +RE+   SMSQEE+DL+EAFRVFDEDGDGYISA ELQVVLRKL L+EGNEIDRVQKMI+S
Sbjct: 121 KRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILS 180

Query: 181 VDRNQDGRVDFFEFKDMMRSVIVRSC 203
           VDRNQDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 VDRNQDGRVDFFEFKDMMRSVIVRSC 201

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BLAST of MS016567.1 vs. NCBI nr
Match: XP_008442311.1 (PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa] >TYK24898.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa])

HSP 1 Score: 318.9 bits (816), Expect = 3.1e-83
Identity = 171/204 (83.82%), Postives = 180/204 (88.24%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKP--SSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKP  SSSSF+LRCPSLNSLRLRRIFD+FDKNGDGMIT EE
Sbjct: 1   MEAVASD-----VPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADES+LDSTVNSFI+PGN GLRFEDFAALHQSLNDTYF G  E E E  
Sbjct: 61  LGQALGLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKR 120

Query: 121 ERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVD 180
           E    SMSQEE+DL+EAFRVFDEDGDGYISA ELQVVLRKL L+EGNEIDRVQKMI+SVD
Sbjct: 121 ETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVD 180

Query: 181 RNQDGRVDFFEFKDMMRSVIVRSC 203
           RNQDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RNQDGRVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of MS016567.1 vs. NCBI nr
Match: XP_023527056.1 (calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.2 bits (801), Expect = 1.7e-81
Identity = 171/207 (82.61%), Postives = 180/207 (86.96%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPSSS--SFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKPSSS  SF+LRCPSLNSLRLRRIFD+FDKNGDGMITTEE
Sbjct: 70  MEAVASD-----VPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEE 129

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADESDLDSTVNSFI PGN GLRFEDFAALHQSLNDTYFG   E E E E
Sbjct: 130 LGQALGLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPVEDEEEKE 189

Query: 121 ERESMSM---SQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIV 180
           E+   +M   SQEE DL+EAFRVFDEDGDGYISA ELQVVLRKL LSEGNE+DRVQKMI+
Sbjct: 190 EKRETNMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMIL 249

Query: 181 SVDRNQDGRVDFFEFKDMMRSVIVRSC 203
           SVD N+DGRVDFFEFKDMMRSVIVRSC
Sbjct: 250 SVDSNRDGRVDFFEFKDMMRSVIVRSC 271

[Close]

BLAST of MS016567.1 vs. NCBI nr
Match: XP_022982604.1 (calcium-binding allergen Bet v 3-like [Cucurbita maxima])

HSP 1 Score: 312.8 bits (800), Expect = 2.2e-81
Identity = 171/207 (82.61%), Postives = 180/207 (86.96%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPSSS--SFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKPSSS  SF+LRCPSLNSLRLRRIFD+FDKNGDGMITTEE
Sbjct: 1   MEAMASD-----VPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADESDLDSTVNSFI PGN GLRFEDFAALHQSLNDTYFG   E E E E
Sbjct: 61  LGQALGLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKE 120

Query: 121 ERESMSM---SQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIV 180
           E+   +M   SQEE DL+EAFRVFDEDGDGYISA ELQVVLRKL LSEGNE+DRVQKMI+
Sbjct: 121 EKRETNMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMIL 180

Query: 181 SVDRNQDGRVDFFEFKDMMRSVIVRSC 203
           SVD N+DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 SVDSNRDGRVDFFEFKDMMRSVIVRSC 202

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BLAST of MS016567.1 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-62
Identity = 132/203 (65.02%), Postives = 160/203 (78.82%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG 60
           ME A   + + PR      +  S+SSF+LR  SLN+LRLRRIFDLFDKN DG+IT +EL 
Sbjct: 8   MEKAGHGHASTPR-----KRSLSNSSFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELS 67

Query: 61  QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYF--GGSEEGEGEGE 120
           +AL LLGL+ D S+L+STV SF + GN GL+FEDF +LHQSLND+YF  GG +E + E +
Sbjct: 68  RALNLLGLETDLSELESTVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDEDDNEED 127

Query: 121 ERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVD 180
            R+S+ +SQEEAD    F+VFDEDGDGYISA ELQ+VL KLG SEG+EIDRV+KMIVSVD
Sbjct: 128 MRKSI-LSQEEADSFGGFKVFDEDGDGYISARELQMVLGKLGFSEGSEIDRVEKMIVSVD 187

Query: 181 RNQDGRVDFFEFKDMMRSVIVRS 202
            N+DGRVDFFEFKDMMRSV+VRS
Sbjct: 188 SNRDGRVDFFEFKDMMRSVLVRS 204

[Close]

BLAST of MS016567.1 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 2.0e-61
Identity = 123/178 (69.10%), Postives = 152/178 (85.39%), Query Frame = 0


Query: 24  SSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFI 83
           SSSF+LR PSLN+LRL+RIFDLFDKNGDG IT EEL QAL  LGL+AD SDL STV S+I
Sbjct: 15  SSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYI 74

Query: 84  QPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDG 143
           QPGN GL F+DF++LH++L+D++FGG+  G GE E+  S S ++ E+DL EAF+VFDE+G
Sbjct: 75  QPGNTGLNFDDFSSLHKTLDDSFFGGA-CGGGENEDDPS-SAAENESDLAEAFKVFDENG 134

Query: 144 DGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMRSVIVRS 202
           DG+ISA ELQ VL+KLGL EG E++RV+KMIVSVDRNQDGRVDFFEFK+MMR+V++ S
Sbjct: 135 DGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRTVVIPS 190

[Close]

BLAST of MS016567.1 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.6e-56
Identity = 117/178 (65.73%), Postives = 139/178 (78.09%), Query Frame = 0


Query: 24  SSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFI 83
           SSSF+LR PSLN+LRL R+FDLFDKN DG IT EEL QAL  LGLDAD SDL STV+SFI
Sbjct: 14  SSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFI 73

Query: 84  QPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDG 143
           +P   GLRF+DFAALH++L++++FG      GEG    S      E+DL EAF VFDEDG
Sbjct: 74  KPDKTGLRFDDFAALHKTLDESFFG------GEG----SCCDGSPESDLEEAFNVFDEDG 133

Query: 144 DGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMRSVIVRS 202
           DG+ISA ELQ VL+KLGL E  EI++V+KMIVSVD N DGRVDFFEFK+MM++V+V S
Sbjct: 134 DGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTVVVPS 181

[Close]

BLAST of MS016567.1 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-54
Identity = 116/182 (63.74%), Postives = 142/182 (78.02%), Query Frame = 0


Query: 20  KKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTV 79
           K  SSSSF+LR PSLNS+RLRRIFD+FD+N D +I+ EEL QAL LLGLDAD S+++S V
Sbjct: 22  KPSSSSSFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIESMV 81

Query: 80  NSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVF 139
              I+P N GLRFEDF  LH+SLND +FG     + E +   +   +Q+E+DL EAF VF
Sbjct: 82  KLHIKPENTGLRFEDFETLHRSLNDVFFG----SKCEDKLGLNPDPAQDESDLKEAFDVF 141

Query: 140 DEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMRSVIV 199
           DE+GDG+ISA ELQVVL KLGL EG+EIDRV+ MI SV+++ DGRVDFFEFKDMMR+VIV
Sbjct: 142 DENGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMMRTVIV 199

Query: 200 RS 202
            S
Sbjct: 202 PS 199

[Close]

BLAST of MS016567.1 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 2.2e-47
Identity = 105/185 (56.76%), Postives = 130/185 (70.27%), Query Frame = 0


Query: 15  QPRLTKKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESD 74
           +P L++KP S SF+LR  SLN+LRLRR+FDLFD+NGDG IT +E+  AL  LGL AD + 
Sbjct: 9   KPSLSRKP-SPSFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASALDALGLGADRAG 68

Query: 75  LDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTE 134
           L++TV  +I  G  GLRF DF ALH++L D  FG  EE     EE        +E D+ E
Sbjct: 69  LEATVGGYIPAGAAGLRFGDFEALHRALGDALFGPVEE-----EEPGKQGEDDDEGDMKE 128

Query: 135 AFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMM 194
           AFRVFDEDGDG+ISAAELQ VL+KLGL E   +  VQ+MI +VDR+ DGRVDF EFK MM
Sbjct: 129 AFRVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGRVDFGEFKCMM 187

Query: 195 RSVIV 200
           + + V
Sbjct: 189 QGITV 187

[Close]

BLAST of MS016567.1 vs. ExPASy TrEMBL
Match: A0A6J1DU76 (calcium-binding protein CML42 OS=Momordica charantia OX=3673 GN=LOC111024052 PE=4 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 3.1e-105
Identity = 201/202 (99.50%), Postives = 201/202 (99.50%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG 60
           MEAAASDNGNAPRPQPRLTK PSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG
Sbjct: 1   MEAAASDNGNAPRPQPRLTKNPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELG 60

Query: 61  QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEER 120
           QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEER
Sbjct: 61  QALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEER 120

Query: 121 ESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRN 180
           ESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRN
Sbjct: 121 ESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 203
           QDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 QDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of MS016567.1 vs. ExPASy TrEMBL
Match: A0A0A0LA10 (Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.1e-85
Identity = 174/206 (84.47%), Postives = 187/206 (90.78%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPS--SSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKPS  SSSF+LRCPSLNSLRLRRIFD+FDKNGDGMITT+E
Sbjct: 1   MEAVASD-----VPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADES+LDSTVNSFI+PGN+GLRFEDFAALHQSLNDTYFGG  E E E E
Sbjct: 61  LGQALGLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDE 120

Query: 121 ERES--MSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVS 180
           +RE+   SMSQEE+DL+EAFRVFDEDGDGYISA ELQVVLRKL L+EGNEIDRVQKMI+S
Sbjct: 121 KRETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILS 180

Query: 181 VDRNQDGRVDFFEFKDMMRSVIVRSC 203
           VDRNQDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 VDRNQDGRVDFFEFKDMMRSVIVRSC 201

[Close]

BLAST of MS016567.1 vs. ExPASy TrEMBL
Match: A0A5A7TQ56 (Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00090 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.5e-83
Identity = 171/204 (83.82%), Postives = 180/204 (88.24%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKP--SSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKP  SSSSF+LRCPSLNSLRLRRIFD+FDKNGDGMIT EE
Sbjct: 1   MEAVASD-----VPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADES+LDSTVNSFI+PGN GLRFEDFAALHQSLNDTYF G  E E E  
Sbjct: 61  LGQALGLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKR 120

Query: 121 ERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVD 180
           E    SMSQEE+DL+EAFRVFDEDGDGYISA ELQVVLRKL L+EGNEIDRVQKMI+SVD
Sbjct: 121 ETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVD 180

Query: 181 RNQDGRVDFFEFKDMMRSVIVRSC 203
           RNQDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RNQDGRVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of MS016567.1 vs. ExPASy TrEMBL
Match: A0A1S3B620 (calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.5e-83
Identity = 171/204 (83.82%), Postives = 180/204 (88.24%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKP--SSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKP  SSSSF+LRCPSLNSLRLRRIFD+FDKNGDGMIT EE
Sbjct: 1   MEAVASD-----VPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADES+LDSTVNSFI+PGN GLRFEDFAALHQSLNDTYF G  E E E  
Sbjct: 61  LGQALGLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYFAGMVEDEDEKR 120

Query: 121 ERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVD 180
           E    SMSQEE+DL+EAFRVFDEDGDGYISA ELQVVLRKL L+EGNEIDRVQKMI+SVD
Sbjct: 121 ETSMSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVD 180

Query: 181 RNQDGRVDFFEFKDMMRSVIVRSC 203
           RNQDGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RNQDGRVDFFEFKDMMRSVIVRSC 199

[Close]

BLAST of MS016567.1 vs. ExPASy TrEMBL
Match: A0A6J1J521 (calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481430 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.1e-81
Identity = 171/207 (82.61%), Postives = 180/207 (86.96%), Query Frame = 0


Query: 1   MEAAASDNGNAPRPQPRLTKKPSSS--SFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEE 60
           MEA ASD      P+PRLTKKPSSS  SF+LRCPSLNSLRLRRIFD+FDKNGDGMITTEE
Sbjct: 1   MEAMASD-----VPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEE 60

Query: 61  LGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGE 120
           LGQALGLLGLDADESDLDSTVNSFI PGN GLRFEDFAALHQSLNDTYFG   E E E E
Sbjct: 61  LGQALGLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKE 120

Query: 121 ERESMSM---SQEEADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIV 180
           E+   +M   SQEE DL+EAFRVFDEDGDGYISA ELQVVLRKL LSEGNE+DRVQKMI+
Sbjct: 121 EKRETNMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMIL 180

Query: 181 SVDRNQDGRVDFFEFKDMMRSVIVRSC 203
           SVD N+DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 SVDSNRDGRVDFFEFKDMMRSVIVRSC 202

[Close]

BLAST of MS016567.1 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 236.9 bits (603), Expect = 1.4e-62
Identity = 123/178 (69.10%), Postives = 152/178 (85.39%), Query Frame = 0


Query: 24  SSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFI 83
           SSSF+LR PSLN+LRL+RIFDLFDKNGDG IT EEL QAL  LGL+AD SDL STV S+I
Sbjct: 15  SSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYI 74

Query: 84  QPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDG 143
           QPGN GL F+DF++LH++L+D++FGG+  G GE E+  S S ++ E+DL EAF+VFDE+G
Sbjct: 75  QPGNTGLNFDDFSSLHKTLDDSFFGGA-CGGGENEDDPS-SAAENESDLAEAFKVFDENG 134

Query: 144 DGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMRSVIVRS 202
           DG+ISA ELQ VL+KLGL EG E++RV+KMIVSVDRNQDGRVDFFEFK+MMR+V++ S
Sbjct: 135 DGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRTVVIPS 190

[Close]

BLAST of MS016567.1 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 219.9 bits (559), Expect = 1.8e-57
Identity = 117/178 (65.73%), Postives = 139/178 (78.09%), Query Frame = 0


Query: 24  SSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFI 83
           SSSF+LR PSLN+LRL R+FDLFDKN DG IT EEL QAL  LGLDAD SDL STV+SFI
Sbjct: 14  SSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFI 73

Query: 84  QPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDG 143
           +P   GLRF+DFAALH++L++++FG      GEG    S      E+DL EAF VFDEDG
Sbjct: 74  KPDKTGLRFDDFAALHKTLDESFFG------GEG----SCCDGSPESDLEEAFNVFDEDG 133

Query: 144 DGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMRSVIVRS 202
           DG+ISA ELQ VL+KLGL E  EI++V+KMIVSVD N DGRVDFFEFK+MM++V+V S
Sbjct: 134 DGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTVVVPS 181

[Close]

BLAST of MS016567.1 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 114.8 bits (286), Expect = 8.2e-26
Identity = 71/186 (38.17%), Postives = 105/186 (56.45%), Query Frame = 0


Query: 10  NAPRPQPRLTKKPSSSSFQLRCPSLNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLD 69
           N+P P P  T  PS SS       ++   L+R+F +FDKNGDG IT EEL  +L  LG+ 
Sbjct: 39  NSP-PSPS-TMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIY 98

Query: 70  ADESDLDSTVNSFIQPGNDGLRFEDFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEE 129
             + DL   ++     G+  +  ++F +L+ S+ D       E   +GE         EE
Sbjct: 99  IPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVD-------EHHNDGE--------TEE 158

Query: 130 ADLTEAFRVFDEDGDGYISAAELQVVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFE 189
            D+ +AF VFD+DGDG+I+  EL+ V+  LGL +G  +D  +KMI+ VD + DGRV++ E
Sbjct: 159 EDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKE 207

Query: 190 FKDMMR 196
           F  MM+
Sbjct: 219 FLQMMK 207

[Close]

BLAST of MS016567.1 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 106.3 bits (264), Expect = 2.9e-23
Identity = 60/162 (37.04%), Postives = 93/162 (57.41%), Query Frame = 0


Query: 34  LNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFE 93
           ++   L+R+F +FDKNGDG IT +EL + L  LG+   + +L   +      G+  +  +
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 94  DFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQ 153
           +F  L++++ D      EE E             EE D+ EAF VFD++GDG+I+  EL+
Sbjct: 61  EFGELYKTIMD------EEDE-------------EEEDMKEAFNVFDQNGDGFITVDELK 120

Query: 154 VVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMR 196
            VL  LGL +G  +D  +KMI  VD + DGRV++ EF+ MM+
Sbjct: 121 AVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of MS016567.1 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 105.5 bits (262), Expect = 5.0e-23
Identity = 59/162 (36.42%), Postives = 94/162 (58.02%), Query Frame = 0


Query: 34  LNSLRLRRIFDLFDKNGDGMITTEELGQALGLLGLDADESDLDSTVNSFIQPGNDGLRFE 93
           ++S  L R+F +FDK+GDG ITT+EL ++   LG+   E +L   +      G+  +  E
Sbjct: 1   MDSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIE 60

Query: 94  DFAALHQSLNDTYFGGSEEGEGEGEERESMSMSQEEADLTEAFRVFDEDGDGYISAAELQ 153
           +F  L++++        E+ +  GEE           D+ EAF VFD +GDG+I+  EL+
Sbjct: 61  EFGELYKTIM------VEDEDEVGEE-----------DMKEAFNVFDRNGDGFITVDELK 120

Query: 154 VVLRKLGLSEGNEIDRVQKMIVSVDRNQDGRVDFFEFKDMMR 196
            VL  LGL +G  ++  +KMI+ VD + DGRV++ EF+ MM+
Sbjct: 121 AVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

The following BLAST results are available for this feature:

BLAST of MS016567.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157322.1	6.4e-105	99.50	calcium-binding protein CML42 [Momordica charantia]	[more]
XP_004137668.1	4.3e-85	84.47	calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein...	[more]
XP_008442311.1	3.1e-83	83.82	PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium...	[more]
XP_023527056.1	1.7e-81	82.61	calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982604.1	2.2e-81	82.61	calcium-binding allergen Bet v 3-like [Cucurbita maxima]	[more]

BLAST of MS016567.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43187	3.1e-62	65.02	Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1	[more]
Q9SVG9	2.0e-61	69.10	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9FI19	2.6e-56	65.73	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q09011	1.4e-54	63.74	Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q10LX4	2.2e-47	56.76	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of MS016567.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DU76	3.1e-105	99.50	calcium-binding protein CML42 OS=Momordica charantia OX=3673 GN=LOC111024052 PE=...	[more]
A0A0A0LA10	2.1e-85	84.47	Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=...	[more]
A0A5A7TQ56	1.5e-83	83.82	Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3B620	1.5e-83	83.82	calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV...	[more]
A0A6J1J521	1.1e-81	82.61	calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC11148143...	[more]

BLAST of MS016567.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20780.1	1.4e-62	69.10	calmodulin like 42	[more]
AT5G44460.1	1.8e-57	65.73	calmodulin like 43	[more]
AT2G43290.1	8.2e-26	38.17	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	2.9e-23	37.04	EF hand calcium-binding protein family	[more]
AT4G03290.1	5.0e-23	36.42	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 38..66 e-value: 5.0E-6 score: 36.0 coord: 169..197 e-value: 7.8E-6 score: 35.4 coord: 131..159 e-value: 1.0E-6 score: 38.3
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 38..67 e-value: 2.9E-8 score: 32.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 130..195 e-value: 2.6E-14 score: 53.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 127..162 score: 16.493979
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 34..69 score: 15.629221
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 165..200 score: 13.369694
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 131..195 e-value: 3.94372E-18 score: 72.9657
None	No IPR available	GENE3D	1.10.238.10		coord: 164..201 e-value: 7.4E-7 score: 31.2
None	No IPR available	GENE3D	1.10.238.10		coord: 121..163 e-value: 4.7E-13 score: 50.5
None	No IPR available	GENE3D	1.10.238.10		coord: 36..104 e-value: 1.6E-13 score: 52.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 15..201
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..201
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 47..59
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 178..190
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 38..195

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS016567	MS016567	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS016567.1-cds	MS016567.1-cds-scaffold1294:128174..128779	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS016567.1	MS016567.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding