MS014158.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS014158.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin-conjugating enzyme like
Location	scaffold5: 1018205 .. 1020122 (-)
Sequence length	456
RNA-Seq Expression	MS014158.1
Synteny	MS014158.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGAGGAATTAATGGCTTCGAAACGTATTCTCAAAGAGCTGAAGGATTTACAGAAGGACCCTCCTACGTCTTGCAGCGCTGGTACGTAATCATCCCCCATTTCCCTTCTATATTTTATTGCGGCACTCTCAGCTCACGTGGTGTGTTCTTGTTTTTGCTCTGAATCTACGATCTTTATTGTACATTATGGAGCAGTCGAATGTTTGTTTCTAATGTGACAACCTATTTTTGGTTCTTTTCTATTTTGAGGTTGTCAAGCATGGTCTGCCCCCTTTTCAACCCGTAATTAATTATTTCTTTCCTCCTCAATGACATTATTTCTTTTGTGGTTGGCTTGCAACTTGCCCGTTGTTTATTCATTTTCAAGTGTTCTCCAATTTAATTTTATTCACGGGGAAAAAATACCTCGAAGTTCATATTGGTTCGTGTTTTTATACTATTTTGATTAGGTCCTGTGGCTGAAGACATGTTTCATTGGCAAGCAACTATCATGGGACCTTCTGACTCTCCATATTCAGGAGGTGTATTCTTAGTTTCTATCCATTTTCCTCCGGACTATCCTTTCAAGCCCCCAAAGGTGAAATGTTGTCTGCTTTTTATGTTTCCGTTGTTATTAATACTTCTTATGTTTGGTATGACCCAAGAATTGACTTCCGTGCTTACTAAAATTAGATGGATGACATTGTGTTTTTGACACCCGATTGTTCCTACTATTCTATATATTAAGATTGTTTCAGTGAGAGTTTTCACGGAGTTTGATGCTGTTGACATTGTTAGTTTAATACTGCAATGTCGTAGAACGGTGTATGGTTCCGAAAGGCTGAACTTTTGAAACCTTGCAGGTTGCTTTTAGGACTAAGGTATTCCACCCGAACATTAATAGCAACGGAAGCATATGTCTTGACATTCTTAAAGAGCAGTGGAGCCCTGCGCTAACTATTTCTAAGGTTAGTCGTAATTTACAGTCTCACGGAACTTAACACATTGAACTAGTTTCTAACTTTGATTAGTAGTGCTTGCGCATGTTCTGTGTTTTGTTGAATTTGGCTGTTTGTTCAAGCGGAATAGAGTAGTGTATTCTCCTTGTCAAAGTAGGGTACAATGAGTGCTCCTGTAGTTTGTTTTTATCAATCTGTCCAGATTTACTTATTTGTTTTCTGTCCTGAAACTGTGAAGTTAGATTGAATGGCCTTGAAATTGATATTCCCGTTTATGGGTTTCTGCACAACATAGAATAAGAAGTTTGTTGTTTCAAATTGTCTTGAATTATTTGGCCTTTGTGAAGTTTAGATTTTCTTGGATATTTTTGTCAGGATATCCCATAAGCAGTGGAGGTGAGGATTATTTGGCGGCATCTGGTTCTCTTAATTCTAGTTCAAAATTTCAAATCTCTCCTAAATTGTTGCATAATTTCAGAAACTGTGTTTGAATGATTTATCATCAAAGGACTTGAACTGTTGACTAGAATTTTTGGGGAAAGCTTAGTATAAGACTGATAATTTAGTTTATGGTTCTTTGGTACCTCCCTTGTATGTGGAACTGGTGTCTAAGACGCCACTGTGTGTTGGTTCTCAAGTGGTTGTTACATACCTATGGAATTTTATTACTTGGTCTAACCTTTAAACTTTTGGCAGTTGTAATTTGTAATTCACTGAAGAAGTCCAGCTGTGGTGTGCTATTACTCTGTCTTTTGTAATTTATCTATTTTTAGCAATCAAATTGATGTTAGAATTTAATTTATAGTAGAACTTTAATACGAACTAAATGTATTTGCAGGTGCTGTTGTCCATATGCTCTCTATTAACCGATCCCAACCCCGATGATCCTCTTGTTCCTGAAATTGCTCACATGTATAAGGCTGATAGAGCCAAGTATGAAGCTACTGCACGCAGCTGGACTCAAAAGTATGCTATGGGA

mRNA sequence

CTCGAGGAATTAATGGCTTCGAAACGTATTCTCAAAGAGCTGAAGGATTTACAGAAGGACCCTCCTACGTCTTGCAGCGCTGGTCCTGTGGCTGAAGACATGTTTCATTGGCAAGCAACTATCATGGGACCTTCTGACTCTCCATATTCAGGAGGTGTATTCTTAGTTTCTATCCATTTTCCTCCGGACTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACTAAGGTATTCCACCCGAACATTAATAGCAACGGAAGCATATGTCTTGACATTCTTAAAGAGCAGTGGAGCCCTGCGCTAACTATTTCTAAGGTGCTGTTGTCCATATGCTCTCTATTAACCGATCCCAACCCCGATGATCCTCTTGTTCCTGAAATTGCTCACATGTATAAGGCTGATAGAGCCAAGTATGAAGCTACTGCACGCAGCTGGACTCAAAAGTATGCTATGGGA

Coding sequence (CDS)

CTCGAGGAATTAATGGCTTCGAAACGTATTCTCAAAGAGCTGAAGGATTTACAGAAGGACCCTCCTACGTCTTGCAGCGCTGGTCCTGTGGCTGAAGACATGTTTCATTGGCAAGCAACTATCATGGGACCTTCTGACTCTCCATATTCAGGAGGTGTATTCTTAGTTTCTATCCATTTTCCTCCGGACTATCCTTTCAAGCCCCCAAAGGTTGCTTTTAGGACTAAGGTATTCCACCCGAACATTAATAGCAACGGAAGCATATGTCTTGACATTCTTAAAGAGCAGTGGAGCCCTGCGCTAACTATTTCTAAGGTGCTGTTGTCCATATGCTCTCTATTAACCGATCCCAACCCCGATGATCCTCTTGTTCCTGAAATTGCTCACATGTATAAGGCTGATAGAGCCAAGTATGAAGCTACTGCACGCAGCTGGACTCAAAAGTATGCTATGGGA

Protein sequence

LEELMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKADRAKYEATARSWTQKYAMG

Homology

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BLAST of MS014158.1 vs. NCBI nr
Match: XP_022149680.1 (ubiquitin-conjugating enzyme E2 28 isoform X1 [Momordica charantia])

HSP 1 Score: 311.2 bits (796), Expect = 4.8e-81
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYKADRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKADRAKYEATARSWTQKYAMG 148

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BLAST of MS014158.1 vs. NCBI nr
Match: XP_012842515.1 (PREDICTED: ubiquitin-conjugating enzyme E2 28 [Erythranthe guttata] >XP_012842583.1 PREDICTED: ubiquitin-conjugating enzyme E2 28 [Erythranthe guttata] >EYU46031.1 hypothetical protein MIMGU_mgv1a015730mg [Erythranthe guttata] >EYU46032.1 hypothetical protein MIMGU_mgv1a015730mg [Erythranthe guttata] >EYU46033.1 hypothetical protein MIMGU_mgv1a015730mg [Erythranthe guttata])

HSP 1 Score: 308.5 bits (789), Expect = 3.1e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK+DRAKYE TARSWTQKYAMG
Sbjct: 121 PEIAHMYKSDRAKYEGTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. NCBI nr
Match: EPS61926.1 (ubiquitin carrier protein [Genlisea aurea])

HSP 1 Score: 308.5 bits (789), Expect = 3.1e-80
Identity = 146/148 (98.65%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKEL+DLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPY+GGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELQDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYTGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYKADRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKADRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. NCBI nr
Match: XP_019432381.1 (PREDICTED: ubiquitin-conjugating enzyme E2 28 [Lupinus angustifolius] >XP_019432388.1 PREDICTED: ubiquitin-conjugating enzyme E2 28 [Lupinus angustifolius] >XP_019432390.1 PREDICTED: ubiquitin-conjugating enzyme E2 28 [Lupinus angustifolius] >XP_019432399.1 PREDICTED: ubiquitin-conjugating enzyme E2 28 [Lupinus angustifolius] >XP_019432406.1 PREDICTED: ubiquitin-conjugating enzyme E2 28 [Lupinus angustifolius] >XP_038901211.1 ubiquitin-conjugating enzyme E2 28 [Benincasa hispida] >KAF1868945.1 hypothetical protein Lal_00048224 [Lupinus albus] >KAF7816246.1 ubiquitin-conjugating enzyme E2-17 kDa [Senna tora] >OIW16104.1 hypothetical protein TanjilG_18819 [Lupinus angustifolius])

HSP 1 Score: 308.5 bits (789), Expect = 3.1e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. NCBI nr
Match: XP_011080642.1 (ubiquitin-conjugating enzyme E2-17 kDa [Sesamum indicum] >XP_021909896.1 ubiquitin-conjugating enzyme E2-17 kDa [Carica papaya] >XP_028768820.1 ubiquitin-conjugating enzyme E2-17 kDa [Prosopis alba] >XP_028768821.1 ubiquitin-conjugating enzyme E2-17 kDa [Prosopis alba] >XP_028774257.1 ubiquitin-conjugating enzyme E2-17 kDa [Prosopis alba])

HSP 1 Score: 308.5 bits (789), Expect = 3.1e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPY+GGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy Swiss-Prot
Match: Q94F47 (Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.2e-82
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy Swiss-Prot
Match: P35135 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 2.7e-82
Identity = 144/148 (97.30%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPY+GGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYAGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYE TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYETTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy Swiss-Prot
Match: P35131 (Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 5.9e-82
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy Swiss-Prot
Match: P35132 (SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 2.3e-81
Identity = 142/148 (95.95%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK D+ KYE+TAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDKNKYESTARTWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy Swiss-Prot
Match: Q8S919 (Ubiquitin-conjugating enzyme E2 5B OS=Oryza sativa subsp. japonica OX=39947 GN=UBC5B PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 3.8e-81
Identity = 141/148 (95.27%), Postives = 146/148 (98.65%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQAT+MGPSDSPY+GGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATLMGPSDSPYAGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVA +TKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVALKTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy TrEMBL
Match: A0A6J1D6E3 (ubiquitin-conjugating enzyme E2 28 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018049 PE=3 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.3e-81
Identity = 148/148 (100.00%), Postives = 148/148 (100.00%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYKADRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKADRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy TrEMBL
Match: A0A5B7AMQ3 (Putative ubiquitin-conjugating enzyme E2 OS=Davidia involucrata OX=16924 GN=Din_027523 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPTDSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy TrEMBL
Match: A0A022S2C1 (UBC core domain-containing protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a015730mg PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK+DRAKYE TARSWTQKYAMG
Sbjct: 121 PEIAHMYKSDRAKYEGTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy TrEMBL
Match: A0A6A5MDS8 (UBC core domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_00048224 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. ExPASy TrEMBL
Match: A0A4P1RQB3 (UBC core domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_18819 PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-80
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP+DSPYSGGVFLVSIHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPADSPYSGGVFLVSIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 307.0 bits (785), Expect = 8.5e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 307.0 bits (785), Expect = 8.5e-84
Identity = 144/148 (97.30%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPN+NSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYE+TARSWTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYESTARSWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. TAIR 10
Match: AT5G41700.2 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 304.7 bits (779), Expect = 4.2e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. TAIR 10
Match: AT5G41700.1 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 304.7 bits (779), Expect = 4.2e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

[Close]

BLAST of MS014158.1 vs. TAIR 10
Match: AT5G41700.5 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 304.7 bits (779), Expect = 4.2e-83
Identity = 143/148 (96.62%), Postives = 147/148 (99.32%), Query Frame = 0


Query: 5   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVSIHFPPDY 64
           MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGP++SPYSGGVFLV+IHFPPDY
Sbjct: 1   MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDY 60

Query: 65  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 124
           PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV
Sbjct: 61  PFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLV 120

Query: 125 PEIAHMYKADRAKYEATARSWTQKYAMG 153
           PEIAHMYK DRAKYEATAR+WTQKYAMG
Sbjct: 121 PEIAHMYKTDRAKYEATARNWTQKYAMG 148

The following BLAST results are available for this feature:

BLAST of MS014158.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149680.1	4.8e-81	100.00	ubiquitin-conjugating enzyme E2 28 isoform X1 [Momordica charantia]	[more]
XP_012842515.1	3.1e-80	98.65	PREDICTED: ubiquitin-conjugating enzyme E2 28 [Erythranthe guttata] >XP_01284258...	[more]
EPS61926.1	3.1e-80	98.65	ubiquitin carrier protein [Genlisea aurea]	[more]
XP_019432381.1	3.1e-80	98.65	PREDICTED: ubiquitin-conjugating enzyme E2 28 [Lupinus angustifolius] >XP_019432...	[more]
XP_011080642.1	3.1e-80	98.65	ubiquitin-conjugating enzyme E2-17 kDa [Sesamum indicum] >XP_021909896.1 ubiquit...	[more]

BLAST of MS014158.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94F47	1.2e-82	97.30	Ubiquitin-conjugating enzyme E2 28 OS=Arabidopsis thaliana OX=3702 GN=UBC28 PE=1...	[more]
P35135	2.7e-82	97.30	Ubiquitin-conjugating enzyme E2-17 kDa OS=Solanum lycopersicum OX=4081 PE=2 SV=1	[more]
P35131	5.9e-82	96.62	Ubiquitin-conjugating enzyme E2 8 OS=Arabidopsis thaliana OX=3702 GN=UBC8 PE=1 S...	[more]
P35132	2.3e-81	95.95	SUMO-conjugating enzyme UBC9 OS=Arabidopsis thaliana OX=3702 GN=UBC9 PE=1 SV=1	[more]
Q8S919	3.8e-81	95.27	Ubiquitin-conjugating enzyme E2 5B OS=Oryza sativa subsp. japonica OX=39947 GN=U...	[more]

BLAST of MS014158.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D6E3	2.3e-81	100.00	ubiquitin-conjugating enzyme E2 28 isoform X1 OS=Momordica charantia OX=3673 GN=...	[more]
A0A5B7AMQ3	1.5e-80	98.65	Putative ubiquitin-conjugating enzyme E2 OS=Davidia involucrata OX=16924 GN=Din_...	[more]
A0A022S2C1	1.5e-80	98.65	UBC core domain-containing protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a...	[more]
A0A6A5MDS8	1.5e-80	98.65	UBC core domain-containing protein OS=Lupinus albus OX=3870 GN=Lal_00048224 PE=3...	[more]
A0A4P1RQB3	1.5e-80	98.65	UBC core domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_1...	[more]

BLAST of MS014158.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64230.1	8.5e-84	97.30	ubiquitin-conjugating enzyme 28	[more]
AT1G64230.2	8.5e-84	97.30	ubiquitin-conjugating enzyme 28	[more]
AT5G41700.2	4.2e-83	96.62	ubiquitin conjugating enzyme 8	[more]
AT5G41700.1	4.2e-83	96.62	ubiquitin conjugating enzyme 8	[more]
AT5G41700.5	4.2e-83	96.62	ubiquitin conjugating enzyme 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 8..151 e-value: 5.5E-80 score: 281.7
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 7..149
None	No IPR available	PANTHER	PTHR24068:SF346	BNAA01G32320D PROTEIN	coord: 7..149
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 2..152 e-value: 4.5E-75 score: 252.6
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 5..151
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 9..145 e-value: 2.0E-53 score: 179.8
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 5..151 score: 60.856739
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 7..146 e-value: 6.10294E-75 score: 218.226
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 78..93

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS014158	MS014158	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS014158.1-cds	MS014158.1-cds-scaffold5:1018205..1018345	CDS
MS014158.1-cds	MS014158.1-cds-scaffold5:1019175..1019279	CDS
MS014158.1-cds	MS014158.1-cds-scaffold5:1019545..1019672	CDS
MS014158.1-cds	MS014158.1-cds-scaffold5:1020041..1020122	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS014158.1	MS014158.1-protein	polypeptide