MS013446.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS013446.1 vs. NCBI nr
Match: XP_038893814.1 (phospholipase A1-Igamma1, chloroplastic [Benincasa hispida])

HSP 1 Score: 935.3 bits (2416), Expect = 2.4e-268
Identity = 462/543 (85.08%), Postives = 497/543 (91.53%), Query Frame = 0

Query: 1   MEATKIATPRPRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLT 60
           MEA K+AT  PRGV +KR TMLRNGTKKTKWYWKLKFGIRL AI+ A+SSSLH  R RLT
Sbjct: 1   MEA-KMAT--PRGVAMKRVTMLRNGTKKTKWYWKLKFGIRLKAIRSAISSSLHNQRRRLT 60

Query: 61  CSTAMNNNGHLTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDY 120
           CSTA+  N   TP NG VSPLV+H S S S   KKNELRLAKPLASLLRMPLRAADFID+
Sbjct: 61  CSTAIPTN---TPVNGIVSPLVVHQSRSSS---KKNELRLAKPLASLLRMPLRAADFIDF 120

Query: 121 GHHMTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDP 180
           G+HMTPTLSPRENI+A+WR+LHG  DWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDP
Sbjct: 121 GNHMTPTLSPRENISAVWRDLHGASDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDP 180

Query: 181 LSDFCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWM 240
           LS+FCGSCRYNRHKL  E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWM
Sbjct: 181 LSEFCGSCRYNRHKLFNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWM 240

Query: 241 GFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFL 300
           GFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R D   K+VKVQRGFL
Sbjct: 241 GFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD---KKVKVQRGFL 300

Query: 301 TIYRSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYE 360
           TIY+SK+E SKFNKTSASEQVMEEL+RL++FFK+KGDREIS++ITGHSLGGAL+LLTAYE
Sbjct: 301 TIYKSKDEESKFNKTSASEQVMEELNRLINFFKEKGDREISLTITGHSLGGALSLLTAYE 360

Query: 361 AGISFP-GVHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVN 420
           AG+ FP GVH+SVVSFGAPRVGNL F+EKL E+GVK LRVVI QDIVPKLPGL VNSIVN
Sbjct: 361 AGVLFPTGVHISVVSFGAPRVGNLTFREKLNEMGVKTLRVVIGQDIVPKLPGLFVNSIVN 420

Query: 421 KLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFR 480
           KLS VT +LNWVYRHVG+ELRMDM MSPYLKRESDM+GSHNLEIYLHLVDGFV  KGKFR
Sbjct: 421 KLSLVTGKLNWVYRHVGKELRMDMYMSPYLKRESDMTGSHNLEIYLHLVDGFVSRKGKFR 480

Query: 481 WNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQP 540
           WNSRRDVALVNKGSDMLVEELRIP+FWYQLPHKGL+KN +GRWVKPGRNPEDIPSPFSQP
Sbjct: 481 WNSRRDVALVNKGSDMLVEELRIPQFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFSQP 531

Query: 541 SKI 543
           S +
Sbjct: 541 SHV 531

BLAST of MS013446.1 vs. NCBI nr
Match: XP_008444745.1 (PREDICTED: phospholipase A1-Igamma1, chloroplastic [Cucumis melo] >KAA0054030.1 phospholipase A1-Igamma1 [Cucumis melo var. makuwa] >TYK20716.1 phospholipase A1-Igamma1 [Cucumis melo var. makuwa])

HSP 1 Score: 934.5 bits (2414), Expect = 4.2e-268
Identity = 460/545 (84.40%), Postives = 500/545 (91.74%), Query Frame = 0

Query: 1   MEATKIATPRPRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLT 60
           MEA K+AT  PRG+PVKR TMLRNGTKKTKWYWKLK GIR  AIK ALSSSLH  R RLT
Sbjct: 1   MEA-KLAT--PRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLT 60

Query: 61  CSTAM--NNNGHLTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFI 120
           CSTA+       LTP NG VSPLVIH    +S  +KKN+LRLAKPLASLLRMPLRAADFI
Sbjct: 61  CSTAITTTTTPQLTPTNGLVSPLVIH----QSKPSKKNDLRLAKPLASLLRMPLRAADFI 120

Query: 121 DYGHHMTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDF 180
           D+G+HMTPTLSPR+NI+A+WR+LHG  DW+GLLDPLHPFLRREIVKYGEFAQATYDAFDF
Sbjct: 121 DFGNHMTPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDF 180

Query: 181 DPLSDFCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN 240
           DPLS+FCGSCRYNRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN
Sbjct: 181 DPLSEFCGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN 240

Query: 241 WMGFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRG 300
           WMG+VAVS+DEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R   S+K+VKVQRG
Sbjct: 241 WMGYVAVSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR---SNKKVKVQRG 300

Query: 301 FLTIYRSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTA 360
           FLTIY+SK+E SKFNKTSASEQVMEELHRL+DFFK+KGDREIS++ITGHSLGGAL+LLTA
Sbjct: 301 FLTIYKSKDEDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTA 360

Query: 361 YEAGISFPG-VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSI 420
           YEAG++FP  VHVSVVSFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSI
Sbjct: 361 YEAGVTFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSI 420

Query: 421 VNKLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGK 480
           VNKL AVT +LNWVYRHVG+ELRM+M MSPYLK++SDMSGSHNLEIYLHLVDGFV  KGK
Sbjct: 421 VNKLGAVTGKLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGK 480

Query: 481 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFS 540
           FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGL+KN +GRWVKPGRNPEDIPSPFS
Sbjct: 481 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFS 535

Query: 541 QPSKI 543
           QP ++
Sbjct: 541 QPLQV 535

BLAST of MS013446.1 vs. NCBI nr
Match: XP_022927629.1 (phospholipase A1-Igamma1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 922.9 bits (2384), Expect = 1.3e-264
Identity = 449/532 (84.40%), Postives = 486/532 (91.35%), Query Frame = 0

Query: 11  PRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLTCSTAMNNNGH 70
           PRGVP+KR TMLRNGTKKTKWYWKLK+GIRL AIK ALSSSLH  R RLTC+TA    G 
Sbjct: 8   PRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATA---TGQ 67

Query: 71  LTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDYGHHMTPTLSP 130
           LTP NGAVSPLVI PS S S   KKN+LRLAKPLASLLRMP RAADF+DYG+HMTPT SP
Sbjct: 68  LTPVNGAVSPLVIQPSKSTS---KKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSP 127

Query: 131 RENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRY 190
           R+ IA +WR+LHGG +W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLS+F GSCRY
Sbjct: 128 RDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRY 187

Query: 191 NRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEE 250
           NRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DEE
Sbjct: 188 NRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEE 247

Query: 251 SDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIYRSKEETS 310
           S+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R D   +++KVQRGFLTIY+SK+E S
Sbjct: 248 SERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD---RKIKVQRGFLTIYKSKDEES 307

Query: 311 KFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYEAGISFPGVHV 370
           KFNKTSASEQVMEELHRL+DFF QKGDREIS++I GHSLGGAL+LLTAYEAG SFP +HV
Sbjct: 308 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 367

Query: 371 SVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKLSAVTRRLNW 430
           SV+SFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSIVNKLSAVT +LNW
Sbjct: 368 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 427

Query: 431 VYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFRWNSRRDVALVN 490
           +YRHVG +L+MDM MSPYLK+ESDMSGSHNLEIYLHLVDGFV  +GKFRWNSRRDVALVN
Sbjct: 428 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 487

Query: 491 KGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQPSKI 543
           KGSDMLVEELRIPEFWYQLPHKGLVKN +GRWVKPGRN EDIPSPFS+PS I
Sbjct: 488 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of MS013446.1 vs. NCBI nr
Match: XP_004135535.1 (phospholipase A1-Igamma1, chloroplastic [Cucumis sativus] >KGN65881.1 hypothetical protein Csa_023302 [Cucumis sativus])

HSP 1 Score: 921.8 bits (2381), Expect = 2.8e-264
Identity = 454/540 (84.07%), Postives = 493/540 (91.30%), Query Frame = 0

Query: 5   KIATPRPRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLTCSTA 64
           K AT  PRG+PVKR TMLRNGTKKTKWYWKLK GI+  AIK ALSSSLH  R RLTCSTA
Sbjct: 4   KFAT--PRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCSTA 63

Query: 65  M-NNNGHLTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDYGHH 124
           +       TP NG VSPLVIH S S    +KKN+LRLAKPLASLLRMPLRA+DFID+G+H
Sbjct: 64  ITTTTPQHTPTNGLVSPLVIHQSKS----SKKNDLRLAKPLASLLRMPLRASDFIDFGNH 123

Query: 125 MTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSD 184
           MTPTLSPR+ I+A+WR+LHG  DW+GLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLS+
Sbjct: 124 MTPTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSE 183

Query: 185 FCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFV 244
           FCGSCRYNR KLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFV
Sbjct: 184 FCGSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFV 243

Query: 245 AVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIY 304
           AVS+DEESDRIGRRDILV+WRGTVTPTEWYIDLKTKLKKI+R   S+K+VKVQRGFLTIY
Sbjct: 244 AVSSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDR---SNKKVKVQRGFLTIY 303

Query: 305 RSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYEAGI 364
           +SK+E SKFNKTSASEQVMEELHRL+DFFKQKGDREIS++ITGHSLGGAL+LLTAYEAG+
Sbjct: 304 KSKDEDSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGV 363

Query: 365 SFPG-VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKLS 424
           +FP  VHVSVVSFGAPRVGNLAF+EKL E+GVK LRVVI QDIVPKLPGL VNSIVNKLS
Sbjct: 364 TFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLS 423

Query: 425 AVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFRWNS 484
           AVT +LNWVYRHVG+ELRM+M MSPYLK++SDMSGSHNLEIYLHLVDGFV  KGKFRWNS
Sbjct: 424 AVTGKLNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNS 483

Query: 485 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQPSKI 543
           RRDVALVNKGSDMLVEELRIPEFWYQLPHKGL+KN +GRWVKPGRN EDIPSPFSQPS++
Sbjct: 484 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of MS013446.1 vs. NCBI nr
Match: KAG7019492.1 (Phospholipase A1-Igamma2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 921.4 bits (2380), Expect = 3.6e-264
Identity = 448/532 (84.21%), Postives = 485/532 (91.17%), Query Frame = 0

Query: 11  PRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLTCSTAMNNNGH 70
           PRGVP+KR TMLRNGTKKTKWYWKLK+GIRL AIK ALSSSLH  R RLTC+TA    G 
Sbjct: 8   PRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATA---TGQ 67

Query: 71  LTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDYGHHMTPTLSP 130
           LTP NGA SPLVI PS S S   KKN+LRLAKPLASLLRMP RAADF+DYG+HMTPT SP
Sbjct: 68  LTPVNGAASPLVIQPSKSTS---KKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSP 127

Query: 131 RENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRY 190
           R+ IA +WR+LHGG +W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLS+F GSCRY
Sbjct: 128 RDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRY 187

Query: 191 NRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEE 250
           NRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DEE
Sbjct: 188 NRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEE 247

Query: 251 SDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIYRSKEETS 310
           S+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R D   +++KVQRGFLTIY+SK+E S
Sbjct: 248 SERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD---RKIKVQRGFLTIYKSKDEES 307

Query: 311 KFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYEAGISFPGVHV 370
           KFNKTSASEQVMEELHRL+DFF QKGDREIS++I GHSLGGAL+LLTAYEAG SFP +HV
Sbjct: 308 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 367

Query: 371 SVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKLSAVTRRLNW 430
           SV+SFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSIVNKLSAVT +LNW
Sbjct: 368 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 427

Query: 431 VYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFRWNSRRDVALVN 490
           +YRHVG +L+MDM MSPYLK+ESDMSGSHNLEIYLHLVDGFV  +GKFRWNSRRDVALVN
Sbjct: 428 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 487

Query: 491 KGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQPSKI 543
           KGSDMLVEELRIPEFWYQLPHKGLVKN +GRWVKPGRN EDIPSPFS+PS I
Sbjct: 488 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of MS013446.1 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 2.9e-107
Identity = 201/411 (48.91%), Postives = 277/411 (67.40%), Query Frame = 0

Query: 138 WRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNRHKLLQ 197
           WR++ G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CG+ R+ R +   
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 198 EVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 257
            +G+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 258 RDILVAWRGTVTPTEWYIDLKTKLKKI--NRDDLSSKRVKVQRGFLTIYRSKEETSKFNK 317
           RDI +AWRGTVT  EW  DLK  LK +  N+       VKV+ GFL +Y  K+ T KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 318 TSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYE-----AGISFPG-- 377
            SA EQ++ E+ RLV+      D ++S+++TGHSLGGALA+L+AY+        S  G  
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 378 VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVN-SIVNKLSAVTR 437
           + V+V+++G PRVGN+ F+E++EELGVK +RVV   D+VPK PGL +N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 438 RLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKG-KFRWNSRRD 497
            L W Y HVG EL +D   SP+LK   D+S +HNLE  LHL+DG+  GKG +F  +S RD
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGY-HGKGERFVLSSGRD 464

Query: 498 VALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGR-NPEDIPSP 536
            ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 465 HALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of MS013446.1 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 1.4e-104
Identity = 191/403 (47.39%), Postives = 267/403 (66.25%), Query Frame = 0

Query: 138 WRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNRHKLLQ 197
           WR++ G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CGSCR+ R  L  
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 198 EVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESD--RIG 257
            +G+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 258 RRDILVAWRGTVTPTEWYIDLKTKLKKINRDDL--SSKRVKVQRGFLTIYRSKEETSKFN 317
           RRDI +AWRGTVT  EW  DLK  LK ++ +        VK + GFL +Y  K+ +  F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 318 KTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAY---EAGISFPG--- 377
           K SA EQV+ E+ RLV+ +  +   E+S+++TGHSLGGALA+L+AY   E G++      
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 378 -VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNS-IVNKLSAVT 437
            + V+  ++G PRVGN+ FKE++E+LGVK LRVV   D+V K PGL +N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 438 RRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKG-KFRWNSRR 497
             L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+  GKG +F  +S R
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGY-HGKGQRFVLSSGR 448

Query: 498 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGR 528
           D ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R
Sbjct: 449 DPALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of MS013446.1 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.5e-100
Identity = 193/429 (44.99%), Postives = 271/429 (63.17%), Query Frame = 0

Query: 133 NIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNR 192
           ++  +WRE+ G  +W+G LDP++  LRREI++YGEFAQA YD+FDFDP S +CGSC+Y+ 
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 193 HKLLQEVGL-AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES 252
                 + L    GY +T+Y+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+TDEE 
Sbjct: 146 SDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEE 205

Query: 253 -DRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIYRSKEETS 312
             R+GRRDI++AWRGTVT  EW  DLK  L   N  D  S  +K++ GF  +Y  KE++ 
Sbjct: 206 VSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPS--IKIELGFHDLYTKKEDSC 265

Query: 313 KFNKTSASEQVMEELHRLVDFF-KQKGDREISVSITGHSLGGALALLTAYE-AGISFPGV 372
           KF+  SA EQV+ E+ RL++++  ++   + S+++TGHSLG +LAL++AY+ A ++   V
Sbjct: 266 KFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHV 325

Query: 373 -------HVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKL 432
                   ++V SF  PRVGNL FKE+ +ELGVK LRVV   D VP +PG+  N      
Sbjct: 326 PENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQ 385

Query: 433 SAVTRRLN--WVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGF----VRGK 492
             V  + +  W Y HVG EL +D   SP+LK   D+  +HNLE  LHLVDG+       +
Sbjct: 386 KYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAE 445

Query: 493 GKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGR------NP 539
            +F   ++RD+ALVNK  D L  E  +P  W Q  +KG+VKNG G+WV P R       P
Sbjct: 446 KRFCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGP 505

BLAST of MS013446.1 vs. ExPASy Swiss-Prot
Match: B9EYD3 (Phospholipase A1-II 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0652300 PE=2 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 2.2e-70
Identity = 165/408 (40.44%), Postives = 226/408 (55.39%), Query Frame = 0

Query: 127 TLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCG 186
           T +PR  +A  WRELHG   W GLLDPL   LRR ++ YGE AQAT DAF  +  S   G
Sbjct: 3   TTAPRA-VAERWRELHGEDHWKGLLDPLDADLRRSVIGYGELAQATNDAFIREAWSPHAG 62

Query: 187 SCRYNRHKLLQEVGLA---QNGYKVTKYIYALSPVDG-PDWFESSKIGEVWSRDSNWMGF 246
           +CRY+R + L++   +      Y+VT + YA +   G P  F       V +R+SNWMG+
Sbjct: 63  ACRYSRDRFLEKAQASTQLAGLYEVTAFFYATAGAGGVPAPF------MVRNRESNWMGY 122

Query: 247 VAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKK---INRDDLSSKRVKVQRGF 306
           VAV+TD     +GRRD++VAWRGTV P EW  DL   L     +      S   +V RG+
Sbjct: 123 VAVATDAGVAALGRRDVVVAWRGTVRPMEWLNDLDFTLVSAAGVLGAGGRSPAPRVHRGW 182

Query: 307 LTIYRSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAY 366
           L+IY + +  SK++K SA EQ+ +E+ RL+D +K   D E S+++ GHSLG A+A L A 
Sbjct: 183 LSIYTASDPASKYSKLSAREQISDEIKRLMDKYK---DEETSITVVGHSLGAAVATLNAA 242

Query: 367 EA---GISFPGV-HVSVVSFGAPRVGNLAFKEKLEEL-GVKALRVVIRQDIVPKLPGLVV 426
           +    G++  G   V+ V+F  PRVG+  F++  +EL G++ LRV    D+VPK P +  
Sbjct: 243 DIVSNGLNQHGACPVTAVAFACPRVGDSGFRKLFDELPGLRLLRVCNSPDVVPKYPPM-- 302

Query: 427 NSIVNKLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRG 486
                            Y  VG EL +D   SPYLK   + +  H+LE Y+H V G    
Sbjct: 303 ----------------GYADVGVELPVDTRRSPYLKSPGNQAVWHSLECYMHGVAGAQGK 362

Query: 487 KGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRW 523
           +G F+    RDVALVNK  D L EE  +P  W     KG+V+   G W
Sbjct: 363 RGGFKLEVDRDVALVNKNVDALKEEYHVPPSWSVQRDKGMVRGADGHW 382

BLAST of MS013446.1 vs. ExPASy Swiss-Prot
Match: Q6F358 (Phospholipase A1-II 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0574000 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.9e-69
Identity = 161/421 (38.24%), Postives = 227/421 (53.92%), Query Frame = 0

Query: 132 ENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYN 191
           +  A  WRELHG  DWDGLLDP    LRR +++YGE AQATYDAF+ + LS   G  R+ 
Sbjct: 9   DGTARRWRELHGESDWDGLLDPFDLDLRRTVIRYGEMAQATYDAFNHEKLSPHAGLSRFA 68

Query: 192 RHKLLQEVGLAQN--GYKVTKYIYALSPVDGPD-WFESSKIGEVWSRDSNWMGFVAVSTD 251
             +  +   L  +   Y+V +++YA S V  P+     S       R+SNW+G+VAV+TD
Sbjct: 69  ARRFFERAQLPGHSAAYRVARFVYATSCVAVPEPLILRSASRARRCRESNWIGYVAVATD 128

Query: 252 EESDRIGRRDILVAWRGTVTPTEWYIDLKTKL---KKINRDDLSSKRVKVQRGFLTIYRS 311
           E    +GRRDI+VAWRGTV   EW  D+   +   K + RD  S     V RG+L++Y S
Sbjct: 129 EGKAALGRRDIVVAWRGTVQSLEWIKDMDFVMVPPKGLLRDKASD--AMVHRGWLSMYTS 188

Query: 312 KEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYE----- 371
           ++  S  NK SA +QV+ E+ +LV  ++   D E+S+++TGHSLG ALA L A++     
Sbjct: 189 RDSESSHNKDSARDQVLSEVAKLVSMYQ---DEELSITVTGHSLGAALATLNAFDIVENG 248

Query: 372 ------AGISFPGVHVSVVSFGAPRVGNLAFKEKLE---ELGVKALRVVIRQDIVPKLPG 431
                 A  +  G  V+   F +PRVG   FK + +    LG++ LRV   +D+VP+ P 
Sbjct: 249 YNRAPRAAAAAAGCPVTAFVFASPRVGGHGFKRRFDGARGLGLRLLRVRNARDVVPRYPP 308

Query: 432 LVVNSIVNKLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGF 491
                               Y  VG EL +D   SPYL+R  +    HNLE YLH V G 
Sbjct: 309 AP-----------------PYHGVGTELAIDTGESPYLRRPGNELVWHNLECYLHGVAGA 368

Query: 492 VRGK-GKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPE 532
             G+ G+F+    RDVAL NK    L +E  +P  W+   ++G+V+   GRW    R  +
Sbjct: 369 RGGEAGRFKLAVERDVALANKSYGALRDEHAVPAGWWIPSNRGMVRGADGRWTLMDREED 407

BLAST of MS013446.1 vs. ExPASy TrEMBL
Match: A0A5A7UFJ9 (Phospholipase A1-Igamma1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001160 PE=4 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 2.0e-268
Identity = 460/545 (84.40%), Postives = 500/545 (91.74%), Query Frame = 0

Query: 1   MEATKIATPRPRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLT 60
           MEA K+AT  PRG+PVKR TMLRNGTKKTKWYWKLK GIR  AIK ALSSSLH  R RLT
Sbjct: 1   MEA-KLAT--PRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLT 60

Query: 61  CSTAM--NNNGHLTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFI 120
           CSTA+       LTP NG VSPLVIH    +S  +KKN+LRLAKPLASLLRMPLRAADFI
Sbjct: 61  CSTAITTTTTPQLTPTNGLVSPLVIH----QSKPSKKNDLRLAKPLASLLRMPLRAADFI 120

Query: 121 DYGHHMTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDF 180
           D+G+HMTPTLSPR+NI+A+WR+LHG  DW+GLLDPLHPFLRREIVKYGEFAQATYDAFDF
Sbjct: 121 DFGNHMTPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDF 180

Query: 181 DPLSDFCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN 240
           DPLS+FCGSCRYNRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN
Sbjct: 181 DPLSEFCGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN 240

Query: 241 WMGFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRG 300
           WMG+VAVS+DEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R   S+K+VKVQRG
Sbjct: 241 WMGYVAVSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR---SNKKVKVQRG 300

Query: 301 FLTIYRSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTA 360
           FLTIY+SK+E SKFNKTSASEQVMEELHRL+DFFK+KGDREIS++ITGHSLGGAL+LLTA
Sbjct: 301 FLTIYKSKDEDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTA 360

Query: 361 YEAGISFPG-VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSI 420
           YEAG++FP  VHVSVVSFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSI
Sbjct: 361 YEAGVTFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSI 420

Query: 421 VNKLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGK 480
           VNKL AVT +LNWVYRHVG+ELRM+M MSPYLK++SDMSGSHNLEIYLHLVDGFV  KGK
Sbjct: 421 VNKLGAVTGKLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGK 480

Query: 481 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFS 540
           FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGL+KN +GRWVKPGRNPEDIPSPFS
Sbjct: 481 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFS 535

Query: 541 QPSKI 543
           QP ++
Sbjct: 541 QPLQV 535

BLAST of MS013446.1 vs. ExPASy TrEMBL
Match: A0A1S3BBX3 (phospholipase A1-Igamma1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487991 PE=4 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 2.0e-268
Identity = 460/545 (84.40%), Postives = 500/545 (91.74%), Query Frame = 0

Query: 1   MEATKIATPRPRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLT 60
           MEA K+AT  PRG+PVKR TMLRNGTKKTKWYWKLK GIR  AIK ALSSSLH  R RLT
Sbjct: 1   MEA-KLAT--PRGIPVKRTTMLRNGTKKTKWYWKLKLGIRFRAIKAALSSSLHNQRRRLT 60

Query: 61  CSTAM--NNNGHLTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFI 120
           CSTA+       LTP NG VSPLVIH    +S  +KKN+LRLAKPLASLLRMPLRAADFI
Sbjct: 61  CSTAITTTTTPQLTPTNGLVSPLVIH----QSKPSKKNDLRLAKPLASLLRMPLRAADFI 120

Query: 121 DYGHHMTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDF 180
           D+G+HMTPTLSPR+NI+A+WR+LHG  DW+GLLDPLHPFLRREIVKYGEFAQATYDAFDF
Sbjct: 121 DFGNHMTPTLSPRDNISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDF 180

Query: 181 DPLSDFCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN 240
           DPLS+FCGSCRYNRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN
Sbjct: 181 DPLSEFCGSCRYNRHKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSN 240

Query: 241 WMGFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRG 300
           WMG+VAVS+DEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R   S+K+VKVQRG
Sbjct: 241 WMGYVAVSSDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDR---SNKKVKVQRG 300

Query: 301 FLTIYRSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTA 360
           FLTIY+SK+E SKFNKTSASEQVMEELHRL+DFFK+KGDREIS++ITGHSLGGAL+LLTA
Sbjct: 301 FLTIYKSKDEDSKFNKTSASEQVMEELHRLIDFFKEKGDREISLTITGHSLGGALSLLTA 360

Query: 361 YEAGISFPG-VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSI 420
           YEAG++FP  VHVSVVSFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSI
Sbjct: 361 YEAGVTFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSI 420

Query: 421 VNKLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGK 480
           VNKL AVT +LNWVYRHVG+ELRM+M MSPYLK++SDMSGSHNLEIYLHLVDGFV  KGK
Sbjct: 421 VNKLGAVTGKLNWVYRHVGKELRMNMYMSPYLKKDSDMSGSHNLEIYLHLVDGFVAKKGK 480

Query: 481 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFS 540
           FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGL+KN +GRWVKPGRNPEDIPSPFS
Sbjct: 481 FRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNPEDIPSPFS 535

Query: 541 QPSKI 543
           QP ++
Sbjct: 541 QPLQV 535

BLAST of MS013446.1 vs. ExPASy TrEMBL
Match: A0A6J1EIJ3 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434398 PE=4 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 6.1e-265
Identity = 449/532 (84.40%), Postives = 486/532 (91.35%), Query Frame = 0

Query: 11  PRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLTCSTAMNNNGH 70
           PRGVP+KR TMLRNGTKKTKWYWKLK+GIRL AIK ALSSSLH  R RLTC+TA    G 
Sbjct: 8   PRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCATA---TGQ 67

Query: 71  LTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDYGHHMTPTLSP 130
           LTP NGAVSPLVI PS S S   KKN+LRLAKPLASLLRMP RAADF+DYG+HMTPT SP
Sbjct: 68  LTPVNGAVSPLVIQPSKSTS---KKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSP 127

Query: 131 RENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRY 190
           R+ IA +WR+LHGG +W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLS+F GSCRY
Sbjct: 128 RDKIADVWRDLHGGSNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEFRGSCRY 187

Query: 191 NRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEE 250
           NRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DEE
Sbjct: 188 NRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSNDEE 247

Query: 251 SDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIYRSKEETS 310
           S+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R D   +++KVQRGFLTIY+SK+E S
Sbjct: 248 SERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD---RKIKVQRGFLTIYKSKDEES 307

Query: 311 KFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYEAGISFPGVHV 370
           KFNKTSASEQVMEELHRL+DFF QKGDREIS++I GHSLGGAL+LLTAYEAG SFP +HV
Sbjct: 308 KFNKTSASEQVMEELHRLIDFFTQKGDREISLTIVGHSLGGALSLLTAYEAGASFPDIHV 367

Query: 371 SVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKLSAVTRRLNW 430
           SV+SFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSIVNKLSAVT +LNW
Sbjct: 368 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 427

Query: 431 VYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFRWNSRRDVALVN 490
           +YRHVG +L+MDM MSPYLK+ESDMSGSHNLEIYLHLVDGFV  +GKFRWNSRRDVALVN
Sbjct: 428 LYRHVGTQLKMDMYMSPYLKKESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 487

Query: 491 KGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQPSKI 543
           KGSDMLVEELRIPEFWYQLPHKGLVKN +GRWVKPGRN EDIPSPFS+PS I
Sbjct: 488 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFSRPSNI 530

BLAST of MS013446.1 vs. ExPASy TrEMBL
Match: A0A0A0M111 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G537320 PE=4 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.4e-264
Identity = 454/540 (84.07%), Postives = 493/540 (91.30%), Query Frame = 0

Query: 5   KIATPRPRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLTCSTA 64
           K AT  PRG+PVKR TMLRNGTKKTKWYWKLK GI+  AIK ALSSSLH  R RLTCSTA
Sbjct: 4   KFAT--PRGIPVKRTTMLRNGTKKTKWYWKLKLGIQFKAIKAALSSSLHNQRRRLTCSTA 63

Query: 65  M-NNNGHLTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDYGHH 124
           +       TP NG VSPLVIH S S    +KKN+LRLAKPLASLLRMPLRA+DFID+G+H
Sbjct: 64  ITTTTPQHTPTNGLVSPLVIHQSKS----SKKNDLRLAKPLASLLRMPLRASDFIDFGNH 123

Query: 125 MTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSD 184
           MTPTLSPR+ I+A+WR+LHG  DW+GLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLS+
Sbjct: 124 MTPTLSPRDKISAVWRDLHGASDWEGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSE 183

Query: 185 FCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFV 244
           FCGSCRYNR KLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFV
Sbjct: 184 FCGSCRYNRQKLLNELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFV 243

Query: 245 AVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIY 304
           AVS+DEESDRIGRRDILV+WRGTVTPTEWYIDLKTKLKKI+R   S+K+VKVQRGFLTIY
Sbjct: 244 AVSSDEESDRIGRRDILVSWRGTVTPTEWYIDLKTKLKKIDR---SNKKVKVQRGFLTIY 303

Query: 305 RSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYEAGI 364
           +SK+E SKFNKTSASEQVMEELHRL+DFFKQKGDREIS++ITGHSLGGAL+LLTAYEAG+
Sbjct: 304 KSKDEDSKFNKTSASEQVMEELHRLIDFFKQKGDREISLTITGHSLGGALSLLTAYEAGV 363

Query: 365 SFPG-VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKLS 424
           +FP  VHVSVVSFGAPRVGNLAF+EKL E+GVK LRVVI QDIVPKLPGL VNSIVNKLS
Sbjct: 364 TFPADVHVSVVSFGAPRVGNLAFREKLNEMGVKILRVVIGQDIVPKLPGLFVNSIVNKLS 423

Query: 425 AVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFRWNS 484
           AVT +LNWVYRHVG+ELRM+M MSPYLK++SDMSGSHNLEIYLHLVDGFV  KGKFRWNS
Sbjct: 424 AVTGKLNWVYRHVGKELRMNMFMSPYLKKDSDMSGSHNLEIYLHLVDGFVTKKGKFRWNS 483

Query: 485 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQPSKI 543
           RRDVALVNKGSDMLVEELRIPEFWYQLPHKGL+KN +GRWVKPGRN EDIPSPFSQPS++
Sbjct: 484 RRDVALVNKGSDMLVEELRIPEFWYQLPHKGLIKNRFGRWVKPGRNVEDIPSPFSQPSQV 534

BLAST of MS013446.1 vs. ExPASy TrEMBL
Match: A0A6J1KL46 (phospholipase A1-Igamma1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495553 PE=4 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.3e-263
Identity = 446/532 (83.83%), Postives = 483/532 (90.79%), Query Frame = 0

Query: 11  PRGVPVKRATMLRNGTKKTKWYWKLKFGIRLNAIKKALSSSLHQHRLRLTCSTAMNNNGH 70
           PRGVP+KR TMLRNGTKKTKWYWKLK+GIRL AIK ALSSSLH  R RLTCSTA    G 
Sbjct: 8   PRGVPLKRVTMLRNGTKKTKWYWKLKWGIRLRAIKNALSSSLHNQRRRLTCSTA---TGQ 67

Query: 71  LTPQNGAVSPLVIHPSNSKSSKNKKNELRLAKPLASLLRMPLRAADFIDYGHHMTPTLSP 130
           LTP NGA SPLVI PS S +   KKN+LRLAKPLASLLRMP RAADF+DYG+HMTPT SP
Sbjct: 68  LTPVNGAASPLVIQPSKSTA---KKNDLRLAKPLASLLRMPQRAADFVDYGNHMTPTFSP 127

Query: 131 RENIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRY 190
           R+ IA +WR+LHG  +W+GLLDPLHPFLRRE+VKYGEFAQATYDAFDFDPLS++ GSCRY
Sbjct: 128 RDKIADVWRDLHGASNWEGLLDPLHPFLRRELVKYGEFAQATYDAFDFDPLSEYRGSCRY 187

Query: 191 NRHKLLQEVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEE 250
           NRHKLL E+GLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVS DEE
Sbjct: 188 NRHKLLHELGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSDDEE 247

Query: 251 SDRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIYRSKEETS 310
           S+RIGRRDILVAWRGTVTPTEWYIDLKTKLKKI+R D   K++KVQRGFLTIY+SK+E S
Sbjct: 248 SERIGRRDILVAWRGTVTPTEWYIDLKTKLKKIDRSD---KKIKVQRGFLTIYKSKDEES 307

Query: 311 KFNKTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYEAGISFPGVHV 370
           KFNKTSASEQVMEELHRL+ FF QKGDREIS++I GHSLGGAL+LLTAYEAG+SFP +HV
Sbjct: 308 KFNKTSASEQVMEELHRLIGFFTQKGDREISLTIVGHSLGGALSLLTAYEAGVSFPDIHV 367

Query: 371 SVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKLSAVTRRLNW 430
           SV+SFGAPRVGNLAF+EKL E+GVK LRVVIRQDIVPKLPGL VNSIVNKLSAVT +LNW
Sbjct: 368 SVISFGAPRVGNLAFREKLNEMGVKTLRVVIRQDIVPKLPGLFVNSIVNKLSAVTGKLNW 427

Query: 431 VYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKGKFRWNSRRDVALVN 490
           +YRHVG +L+MDM MSPYLKRESDMSGSHNLEIYLHLVDGFV  +GKFRWNSRRDVALVN
Sbjct: 428 LYRHVGTQLKMDMYMSPYLKRESDMSGSHNLEIYLHLVDGFVSRRGKFRWNSRRDVALVN 487

Query: 491 KGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGRNPEDIPSPFSQPSKI 543
           KGSDMLVEELRIPEFWYQLPHKGLVKN +GRWVKPGRN EDIPSPF +PS I
Sbjct: 488 KGSDMLVEELRIPEFWYQLPHKGLVKNRFGRWVKPGRNAEDIPSPFPRPSNI 530

BLAST of MS013446.1 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 592.0 bits (1525), Expect = 4.7e-169
Identity = 283/439 (64.46%), Postives = 353/439 (80.41%), Query Frame = 0

Query: 102 KPLASLLRMPLRAA-DFIDYGHHMTPTLSPRENIAALWRELHGGRDWDGLLDPLHPFLRR 161
           K LA LL++P  +A DF+D G  MTP  SPRE I+ +WRELHG  +W+ LLDPLHP+LRR
Sbjct: 81  KSLAFLLQLPKYSADDFLDRGDLMTPAASPREKISKMWRELHGSNNWENLLDPLHPWLRR 140

Query: 162 EIVKYGEFAQATYDAFDFDPLSDFCGSCRYNRHKLLQEVGLAQNGYKVTKYIYALSPVDG 221
           E+ KYGEF ++ YD+ DFDPLS+FCGS RYNR+KL +E+GL ++GYKVTKYIYA+S VD 
Sbjct: 141 EVTKYGEFVESVYDSLDFDPLSEFCGSSRYNRNKLFEELGLTRHGYKVTKYIYAMSRVDV 200

Query: 222 PDWFESSKIGEVWSRDSNWMGFVAVSTDEESDRIGRRDILVAWRGTVTPTEWYIDLKTKL 281
           P WF SS +GE WS+DSNWMGFVAVS D ES RIGRRDI+VAWRGTVTPTEW++DL+T +
Sbjct: 201 PQWFLSSALGETWSKDSNWMGFVAVSGDRESLRIGRRDIVVAWRGTVTPTEWFMDLRTSM 260

Query: 282 KKINRDDLSSKR-VKVQRGFLTIYRSKEETSKFNKTSASEQVMEELHRLVDFFKQKGDRE 341
           +  + +    K  VKVQ GFL+IY SK E +++NK SASEQ M+E+ RLV+FFK +G+ E
Sbjct: 261 EPFDCEGKHGKTVVKVQSGFLSIYNSKSELTRYNKESASEQTMDEVKRLVNFFKDRGE-E 320

Query: 342 ISVSITGHSLGGALALLTAYEAGISFPGV--HVSVVSFGAPRVGNLAFKEKLEELGVKAL 401
           +S++ITGHSLGGALAL+ AYEA    P +  ++SV+SFGAPRVGNLAFKEKL  LGVK L
Sbjct: 321 VSLTITGHSLGGALALMNAYEAARDVPALSGNISVISFGAPRVGNLAFKEKLNSLGVKVL 380

Query: 402 RVVIRQDIVPKLPGLVVNSIVNKLSAVTRRLNWVYRHVGRELRMDMTMSPYLKRESDMSG 461
           RVV +QDIVPKLPG+V N ++NKL+ +T RLNWVYRHVG +L++D+  SPY+KR+SD+  
Sbjct: 381 RVVNKQDIVPKLPGIVFNKVLNKLNPITSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGR 440

Query: 462 SHNLEIYLHLVDGFVRGKGKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKN 521
           +HNLE+YLH++DGF R K  FR N+RRDVA VNK +DML++ LRIPEFWYQ+ HKGL+ N
Sbjct: 441 AHNLEVYLHVLDGFHRKKSGFRVNARRDVASVNKSTDMLLDHLRIPEFWYQVAHKGLILN 500

Query: 522 GY-GRWVKPGRNPEDIPSP 536
              GRWVKP R PEDIPSP
Sbjct: 501 KQTGRWVKPVRAPEDIPSP 518

BLAST of MS013446.1 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 390.6 bits (1002), Expect = 2.1e-108
Identity = 201/411 (48.91%), Postives = 277/411 (67.40%), Query Frame = 0

Query: 138 WRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNRHKLLQ 197
           WR++ G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CG+ R+ R +   
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 198 EVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 257
            +G+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 258 RDILVAWRGTVTPTEWYIDLKTKLKKI--NRDDLSSKRVKVQRGFLTIYRSKEETSKFNK 317
           RDI +AWRGTVT  EW  DLK  LK +  N+       VKV+ GFL +Y  K+ T KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 318 TSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYE-----AGISFPG-- 377
            SA EQ++ E+ RLV+      D ++S+++TGHSLGGALA+L+AY+        S  G  
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 378 VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVN-SIVNKLSAVTR 437
           + V+V+++G PRVGN+ F+E++EELGVK +RVV   D+VPK PGL +N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 438 RLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKG-KFRWNSRRD 497
            L W Y HVG EL +D   SP+LK   D+S +HNLE  LHL+DG+  GKG +F  +S RD
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGY-HGKGERFVLSSGRD 464

Query: 498 VALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGR-NPEDIPSP 536
            ALVNK SD L E L+IP FW Q  +KG+V+N  GRW++  R   ED  SP
Sbjct: 465 HALVNKASDFLKEHLQIPPFWRQDANKGMVRNSEGRWIQAERLRFEDHHSP 514

BLAST of MS013446.1 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 381.7 bits (979), Expect = 9.7e-106
Identity = 191/403 (47.39%), Postives = 267/403 (66.25%), Query Frame = 0

Query: 138 WRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNRHKLLQ 197
           WR++ G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CGSCR+ R  L  
Sbjct: 89  WRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTRRHLFD 148

Query: 198 EVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEESD--RIG 257
            +G+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS D E+   R+G
Sbjct: 149 SLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEATRCRLG 208

Query: 258 RRDILVAWRGTVTPTEWYIDLKTKLKKINRDDL--SSKRVKVQRGFLTIYRSKEETSKFN 317
           RRDI +AWRGTVT  EW  DLK  LK ++ +        VK + GFL +Y  K+ +  F+
Sbjct: 209 RRDIAIAWRGTVTRLEWIADLKDFLKPVSGNGFRCPDPAVKAESGFLDLYTDKDTSCNFS 268

Query: 318 KTSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAY---EAGISFPG--- 377
           K SA EQV+ E+ RLV+ +  +   E+S+++TGHSLGGALA+L+AY   E G++      
Sbjct: 269 KFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVNRTRKGK 328

Query: 378 -VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNS-IVNKLSAVT 437
            + V+  ++G PRVGN+ FKE++E+LGVK LRVV   D+V K PGL +N      L  + 
Sbjct: 329 VIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERAPQALMKLA 388

Query: 438 RRLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFVRGKG-KFRWNSRR 497
             L W Y HVG  L +D   SP+LK   D+S +HNLE  LHL+DG+  GKG +F  +S R
Sbjct: 389 GGLPWCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLLDGY-HGKGQRFVLSSGR 448

Query: 498 DVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGR 528
           D ALVNK SD L +   +P +W Q  +KG+V+N  GRW++P R
Sbjct: 449 DPALVNKASDFLKDHFMVPPYWRQDANKGMVRNTDGRWIQPDR 490

BLAST of MS013446.1 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 367.1 bits (941), Expect = 2.5e-101
Identity = 193/429 (44.99%), Postives = 271/429 (63.17%), Query Frame = 0

Query: 133 NIAALWRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNR 192
           ++  +WRE+ G  +W+G LDP++  LRREI++YGEFAQA YD+FDFDP S +CGSC+Y+ 
Sbjct: 86  SLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHP 145

Query: 193 HKLLQEVGL-AQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES 252
                 + L    GY +T+Y+YA S ++ P++F+ SK+  +WS+ +NWMGFVAV+TDEE 
Sbjct: 146 SDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDEEE 205

Query: 253 -DRIGRRDILVAWRGTVTPTEWYIDLKTKLKKINRDDLSSKRVKVQRGFLTIYRSKEETS 312
             R+GRRDI++AWRGTVT  EW  DLK  L   N  D  S  +K++ GF  +Y  KE++ 
Sbjct: 206 VSRLGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPS--IKIELGFHDLYTKKEDSC 265

Query: 313 KFNKTSASEQVMEELHRLVDFF-KQKGDREISVSITGHSLGGALALLTAYE-AGISFPGV 372
           KF+  SA EQV+ E+ RL++++  ++   + S+++TGHSLG +LAL++AY+ A ++   V
Sbjct: 266 KFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHV 325

Query: 373 -------HVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVNSIVNKL 432
                   ++V SF  PRVGNL FKE+ +ELGVK LRVV   D VP +PG+  N      
Sbjct: 326 PENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQ 385

Query: 433 SAVTRRLN--WVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGF----VRGK 492
             V  + +  W Y HVG EL +D   SP+LK   D+  +HNLE  LHLVDG+       +
Sbjct: 386 KYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGYHGKDEEAE 445

Query: 493 GKFRWNSRRDVALVNKGSDMLVEELRIPEFWYQLPHKGLVKNGYGRWVKPGR------NP 539
            +F   ++RD+ALVNK  D L  E  +P  W Q  +KG+VKNG G+WV P R       P
Sbjct: 446 KRFCLVTKRDIALVNKSCDFLRGEYHVPPCWRQDENKGMVKNGDGQWVLPDRPLLEPHGP 505

BLAST of MS013446.1 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 4.2e-93
Identity = 168/346 (48.55%), Postives = 236/346 (68.21%), Query Frame = 0

Query: 138 WRELHGGRDWDGLLDPLHPFLRREIVKYGEFAQATYDAFDFDPLSDFCGSCRYNRHKLLQ 197
           WR++ G  DW GL+DP+ P LR E+++YGE AQA YDAFDFDP S +CG+ R+ R +   
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 198 EVGLAQNGYKVTKYIYALSPVDGPDWFESSKIGEVWSRDSNWMGFVAVSTDEES-DRIGR 257
            +G+  +GY+V +Y+YA S ++ P++F  S+  +VWS+++NWMG+VAVS DE S +R+GR
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSRNRLGR 224

Query: 258 RDILVAWRGTVTPTEWYIDLKTKLKKI--NRDDLSSKRVKVQRGFLTIYRSKEETSKFNK 317
           RDI +AWRGTVT  EW  DLK  LK +  N+       VKV+ GFL +Y  K+ T KF +
Sbjct: 225 RDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFAR 284

Query: 318 TSASEQVMEELHRLVDFFKQKGDREISVSITGHSLGGALALLTAYE-----AGISFPG-- 377
            SA EQ++ E+ RLV+      D ++S+++TGHSLGGALA+L+AY+        S  G  
Sbjct: 285 FSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGKV 344

Query: 378 VHVSVVSFGAPRVGNLAFKEKLEELGVKALRVVIRQDIVPKLPGLVVN-SIVNKLSAVTR 437
           + V+V+++G PRVGN+ F+E++EELGVK +RVV   D+VPK PGL +N S  + L  +  
Sbjct: 345 IPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIAE 404

Query: 438 RLNWVYRHVGRELRMDMTMSPYLKRESDMSGSHNLEIYLHLVDGFV 473
            L W Y HVG EL +D   SP+LK   D+S +HNLE  LHL+DG+V
Sbjct: 405 GLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYV 450