MS012480.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS012480.1 vs. NCBI nr
Match: XP_022149315.1 (kinetochore protein NDC80 homolog [Momordica charantia])

HSP 1 Score: 1055.4 bits (2728), Expect = 1.7e-304
Identity = 563/565 (99.65%), Postives = 563/565 (99.65%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS
Sbjct: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS
Sbjct: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE
Sbjct: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEA AEALRTEPSQRE
Sbjct: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEANAEALRTEPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQE KRICEENEELKM
Sbjct: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQEKKRICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME
Sbjct: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR
Sbjct: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
           QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI
Sbjct: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDMKSNLSKTAIGISEAYKNSLPAE
Sbjct: 541 SDMKSNLSKTAIGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. NCBI nr
Match: XP_022984383.1 (kinetochore protein NDC80 homolog [Cucurbita maxima])

HSP 1 Score: 899.4 bits (2323), Expect = 1.5e-257
Identity = 475/565 (84.07%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRR PK SFNPPPPPTPQFRRDSDASFASSRPSSIGMAR AAASID+YKERAVQ S
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVN INSSLKELF+HSFN+TFKP +P SLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVAVYN +LANDS SF+++N VNVY L 
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ VESL  L+ +V+ELEAKAEALR++PSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQ+VLEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QENK+ICEENEELKM
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFN RDVERMRRELQAVERDIGD EVSRN+WEEKTWD+DAT+G+KFKELEAMA E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNC QY LNAKGSTPA++MG+DYK+T+KPALDS A+DIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAK+ESRR +I SLQSHI+E ETQVNSL+NE +DY LRCSTE+KKMVEDV+
Sbjct: 421 SLRQQSSENTAKIESRRSHILSLQSHIEEAETQVNSLKNEIRDYTLRCSTESKKMVEDVQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
            EAH++D++EREAGEILKNAKLKH+EA+KQ EEEI ICAREL+A VDSVS+Y+E VRSKI
Sbjct: 481 LEAHSIDVVEREAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+SNLSKTA+GISEAYKNSLPAE
Sbjct: 541 SDMRSNLSKTAVGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. NCBI nr
Match: XP_023553517.1 (kinetochore protein NDC80 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 899.0 bits (2322), Expect = 2.0e-257
Identity = 474/565 (83.89%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRR PK SFNPPPPPTPQFRRDSDASFASSRPSSIGMAR AAASID+YKERAVQ S
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVN INSSLKELF+HSFN+TFKP +P SLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVAVYN +LANDS SF+++N VNVY L 
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ VESL  L+ +V+ELEAKAEALR++PSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQ+VLEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QENK+ICEENEELKM
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFN RDVERMRRELQAVERDIGD EVSRN+WEEKTWD+DAT+G+KFKELEAMA E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNC QY LNAKGSTPA++MG+DYK+T+KPALDS A+DIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAK+ESRR +I SLQSHI+E+ETQVNSL+NE +DY LRCSTE+KKMVEDV+
Sbjct: 421 SLRQQSSENTAKIESRRSHIVSLQSHIEEVETQVNSLKNEIRDYTLRCSTESKKMVEDVQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
            EAH++D++E EAGEILKNAKLKH+EA+KQ EEEI ICAREL+A VDSVS+Y+E VRSKI
Sbjct: 481 LEAHSIDVVESEAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+SNLSKTA+GISEAYKNSLPAE
Sbjct: 541 SDMRSNLSKTAVGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. NCBI nr
Match: KAG6576966.1 (Kinetochore protein NDC80-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014987.1 Kinetochore protein NDC80-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897.9 bits (2319), Expect = 4.5e-257
Identity = 472/565 (83.54%), Postives = 525/565 (92.92%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRR PK SFNPPPPPTPQFRRDSDASFASSRPSSIGMAR AAASID+YKERAVQ S
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVN INSSLKELF+HSFN+TFKP +P SLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVAVYN +LANDS SF+++N VNVY L 
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ VESL  L+ +V+ELEAKAEALR++PSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQ+VLEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QENK+ICEENEELKM
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFN RDVERMRRELQAVERDIGD EVSRN+WEEKTWD+DAT+G+KFKELEAMA E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNC QY LNAKGSTPA++MG+DYK+T+KP+LDS A+DIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPSLDSFAEDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAK+ESRR +I SLQSHI+E+ETQVNSLRNE +DY LRCSTE+KKMVED++
Sbjct: 421 SLRQQSSENTAKIESRRSHILSLQSHIEEVETQVNSLRNEIRDYTLRCSTESKKMVEDLQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
            EAH++D++EREAGE+LKNAK+KH+EA+KQ EEEI ICAREL+A VDSVS+Y+E VRSKI
Sbjct: 481 LEAHSIDVVEREAGELLKNAKVKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+SNLSKTA+GISEAYKNSLPAE
Sbjct: 541 SDMRSNLSKTAVGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. NCBI nr
Match: XP_022923126.1 (kinetochore protein NDC80 homolog [Cucurbita moschata])

HSP 1 Score: 892.9 bits (2306), Expect = 1.4e-255
Identity = 471/565 (83.36%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRR PK SFNPPPPPTPQFRRDSDASFASSRPSSIGMAR AAASID+YKERAVQ S
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVN INSSLKELF+HSFN+TFKP +P SLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVAVYN +LANDS SF+++N VNVY L 
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ VESL  L+ +V+ELEAKAEALR++PSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQ+VLEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QENK+ICEENEELKM
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIGGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFN RDVERMRRELQAVERDIGD EVSRN+WEEKTWD+DAT+G+KFKELEAMA E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNC QY LNAKGSTPA++MG+DYK+T+KP+LDS A+DIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPSLDSFAEDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSEN+AK+ESRR +I SLQSHI+E ETQVNSLRNE +DY LRCSTE+KKMVED++
Sbjct: 421 SLRQQSSENSAKIESRRSHILSLQSHIEEGETQVNSLRNEIRDYTLRCSTESKKMVEDLQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
            EAH++D++EREAGE+LKNAK+KH+EA+KQ EEEI ICAREL+A VDSVS+Y+E VRSKI
Sbjct: 481 LEAHSIDVVEREAGELLKNAKVKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+SNLSKTA+GISEAYKNSLPAE
Sbjct: 541 SDMRSNLSKTAVGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. ExPASy Swiss-Prot
Match: Q9M1G5 (Kinetochore protein NDC80 homolog OS=Arabidopsis thaliana OX=3702 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 1.2e-122
Identity = 258/572 (45.10%), Postives = 388/572 (67.83%), Query Frame = 0

Query: 1   MRG--AGRRRPKESFN---PPPPPTPQFR------RDSDASFASSRPSSIGMARSAAASI 60
           MRG  AG+RR    F    PPPPP+ + +      RDSDASFASSRPSSIG+    A+  
Sbjct: 1   MRGGAAGKRRTTVGFGGAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASD- 60

Query: 61  DIYKERAVQHSAVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYP--P 120
               +R+     +NA       L +H+F ++ + +   S+KD++ET+  LL  LDYP   
Sbjct: 61  ----DRSSMIRFINAF------LSTHNFPISIRGNPVPSVKDISETLKFLLSALDYPCDS 120

Query: 121 TKLEDDLPIVLKSLGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHS 180
            K ++DL   LKS   PFKI KS LK P  PH W ++LA++HWL ++A ++  L ++S S
Sbjct: 121 IKWDEDLVFFLKSQKCPFKITKSSLKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTS 180

Query: 181 FITANHVNVYALESYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEA 240
               N +N +A++S+ H+IRG+DD V DLD +F+ +LE E+ +  E++   ++   ELEA
Sbjct: 181 VPEDNSMNFFAIQSFGHFIRGEDDKVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEA 240

Query: 241 KAEALRTEPSQREVLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQE 300
           K E+LR  PS++E LEK ++ LE DV KF T++  Y+++   M+K++EEK KEL+ K +E
Sbjct: 241 KLESLRKGPSKKESLEKVKADLENDVNKFRTIVVEYTDRNPAMEKVVEEKAKELKAKEEE 300

Query: 301 NKRICEENEELKMRVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATL 360
            +RI  EN+ELK  VE Q F+  DV RMRRELQAVERD+ D EV+R+ W++K W+L++ +
Sbjct: 301 RERISVENKELKKSVELQNFSAADVNRMRRELQAVERDVADAEVARDGWDQKAWELNSQI 360

Query: 361 GHKFKELEAMAMECNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADD 420
            ++F +++ +A++CNQA+RRLKLD   Q+ +N +G TPA +MG+DYKS VKPAL SL D 
Sbjct: 361 RNQFHQIQTLAIDCNQALRRLKLD--IQFAVNERGETPAAVMGVDYKSVVKPALCSLCDG 420

Query: 421 IKKSSMTKLEDLISLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLR 480
           IK SS  K+E+L++L+   SE  +K+ES+R  + S+Q  I+++E ++  ++ ETQ+   +
Sbjct: 421 IKGSSAEKVEELVTLQHHKSEMASKIESKRSLLGSIQLQINDLEEKMKLVKKETQELSTK 480

Query: 481 CSTEAKKMVEDVRQEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVD 540
           C  EAK +VE V+ EA NL+++E+EA E +K ++L+ QEAVK+SEEE+Q CA +L A +D
Sbjct: 481 CDLEAKTLVESVKAEALNLEVVEKEAAEFVKASELRLQEAVKESEEEVQACAAQLFALID 540

Query: 541 SVSKYQESVRSKISDMKSNLSKTAIGISEAYK 560
           S+SK +E + SKIS++K+ ++ TA  +SE YK
Sbjct: 541 SISKQKEYMDSKISEIKTGVADTASAVSEIYK 559

BLAST of MS012480.1 vs. ExPASy Swiss-Prot
Match: Q76I89 (Kinetochore protein NDC80 homolog OS=Gallus gallus OX=9031 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.7e-17
Identity = 120/530 (22.64%), Postives = 234/530 (44.15%), Query Frame = 0

Query: 75  HSFNVTFK----PSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKSLGYPFKINKS 134
           ++ NVT K    PS    +K  T     L    + P +K E+++P V K LGYPF ++KS
Sbjct: 111 YAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKS 170

Query: 135 ILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSF-------------ITANHVNV- 194
            + T  APH W  I+A L WL        ++  +  SF             I  N + + 
Sbjct: 171 SMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMD 230

Query: 195 YALESYVHYIRGDDDSVEDLDQKFMERLE---KERDNTVESLGELKQQVEELEAKAEALR 254
           Y ++ Y H+++G  D+ E+LD +   +L+      +  +E L    +++ E  A+ E  R
Sbjct: 231 YVVKCYEHFMKG-GDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKER 290

Query: 255 -TEPSQREVLEKQQSVLEEDVKKFHTMITGYSEKV----QEMKKILEEKE-KELEVKAQE 314
            +EP +   L K +S  + DV+K+   +           Q+MK + EE E  E+EV+A +
Sbjct: 291 ESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMK 350

Query: 315 NKRICEENEELKMRVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATL 374
                +EN  L+   ++Q ++  D+ER+  E   +++ I        A E + W+ +   
Sbjct: 351 -----QENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKY 410

Query: 375 GHKFKELEAMAMECNQAMRRLKL-----DNCRQYILNAKGSTPA-ELMGIDYKSTVKPAL 434
               + +E    E ++  R+LKL     +N + +      +  A     + Y++ +K  L
Sbjct: 411 ARNKEAIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCLVKYRTQIKAPL 470

Query: 435 DSLADDIKKSSMTKLEDLISLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNET 494
             + +  ++      +  +SL     +  A +  ++ ++ +L+   ++++   +    E 
Sbjct: 471 MEIVNQTEEEIRKATQRKMSLEDTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEA 530

Query: 495 QDYRLRCSTEAKKMVEDVRQEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARE 554
           ++   +C+ E + + +  +     ++    EA   L + + ++Q  ++ + EE +     
Sbjct: 531 EEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDN 590

Query: 555 L-------LAWVDSVSKYQESVRSKISD-----MKSNLSKTAIGISEAYK 560
           L          V S+ KY +   SKI       M  +L  T  GI ++YK
Sbjct: 591 LNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYK 634

BLAST of MS012480.1 vs. ExPASy Swiss-Prot
Match: O14777 (Kinetochore protein NDC80 homolog OS=Homo sapiens OX=9606 GN=NDC80 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.7e-15
Identity = 112/521 (21.50%), Postives = 231/521 (44.34%), Query Frame = 0

Query: 75  HSFNVTFK----PSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKSLGYPFKINKS 134
           ++ NV+ K    PS    LK  T     L    + P TK E+++P + K LGYPF ++KS
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSF-------------ITANHVNV- 194
            + T  APH W  I+A L WL+     + ++   S  F             I  N + + 
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLD 227

Query: 195 YALESYVHYIRGDDDSVEDLDQKFMERLEKERDNT----VESLGELKQQVEELEAKAEAL 254
           Y ++ Y  ++ G  DS ++++ +   +L K+  N     +ESL    + + E  A+ E  
Sbjct: 228 YTIKCYESFMSG-ADSFDEMNAELQSKL-KDLFNVDAFKLESLEAKNRALNEQIARLEQE 287

Query: 255 R-TEPSQREVLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEK----EKELEVKAQE 314
           R  EP++ E L K ++ L+ DV+K+      Y   ++    IL++K     +E+     E
Sbjct: 288 REKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELE 347

Query: 315 NKRICEENEELKMRVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATL 374
            + I +EN  L+  +++Q ++  D+ER+  E   +++ I        A ++K W+ +   
Sbjct: 348 CETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKY 407

Query: 375 GHKFKELEAMAMECNQAMRRLKL-----DNCRQYILNAKGSTPAEL-MGIDYKSTVKPAL 434
               + +E    E ++  R+LKL     +N + Y    K +  A     + Y++ V   L
Sbjct: 408 ARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPL 467

Query: 435 DSLADDIKKSSMTKLEDLISLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNET 494
             L ++ ++     L   + L     +  A +   ++++ +L+  + +++        E 
Sbjct: 468 KELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEA 527

Query: 495 QDYRLRCSTEAKKMVEDVRQEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARE 554
           ++   +C++E    +E + +  H   L+E    + L  A  +     ++ +  +Q    E
Sbjct: 528 EEEDEKCASE----LESLEKHKH---LLESTVNQGLSEAMNELDAVQREYQLVVQTTTEE 587

Query: 555 LLAWVDSVSKYQESVRSKISDMKSNLSKTAIGISEAYKNSL 563
                +++ +  E V + +  ++ +L +    +   Y+  +
Sbjct: 588 RRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECM 615

BLAST of MS012480.1 vs. ExPASy Swiss-Prot
Match: Q4R630 (Kinetochore protein NDC80 homolog OS=Macaca fascicularis OX=9541 GN=NDC80 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.8e-14
Identity = 110/501 (21.96%), Postives = 223/501 (44.51%), Query Frame = 0

Query: 75  HSFNVTFK----PSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKSLGYPFKINKS 134
           ++ NV+ K    PS    LK  T     L    + P TK E+++P + K LGYPF ++KS
Sbjct: 108 YAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKS 167

Query: 135 ILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSF-------------ITANHVNV- 194
            + T  APH W  I+A L WL+     + ++   S  F             I  N + + 
Sbjct: 168 SMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLD 227

Query: 195 YALESYVHYIRGDDDSVEDLDQKFMERLEKERDNT----VESLGELKQQVEELEAKAEAL 254
           Y ++ Y  ++ G  DS ++++ +   +L K+  N     +ESL    + + E  A+ E  
Sbjct: 228 YTIKCYESFMSG-ADSFDEMNAELQSKL-KDLFNVDAFKLESLEAKNRALNEQIARLEQE 287

Query: 255 R-TEPSQREVLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEK----EKELEVKAQE 314
           R  EP++ E L K ++ L+ DV+K+      Y   ++    IL++K     +E+     E
Sbjct: 288 REKEPNRLESLRKLKASLQGDVQKYQ----AYMSNLESHSAILDQKLNGLNEEIARVELE 347

Query: 315 NKRICEENEELKMRVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATL 374
            + I +EN  L+  +++Q ++  D+ER+  E   +++ I        A ++K W+ +   
Sbjct: 348 CETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKY 407

Query: 375 GHKFKELEAMAMECNQAMRRLKL-----DNCRQYILNAKGSTPAEL-MGIDYKSTVKPAL 434
               + +E    E ++  R+LKL     +N + Y    K +  A     + Y++ V   L
Sbjct: 408 ARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPL 467

Query: 435 DSLADDIKKSSMTKLEDLISLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNET 494
             L ++ ++         + L     +  A +   ++++ +L+  + +++        E 
Sbjct: 468 KELLNETEEEINKARNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEA 527

Query: 495 QDYRLRCSTEAKKMVEDVRQEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARE 543
           ++   +C++E + + +        ++    EA   L   + ++Q  V+ + EE +     
Sbjct: 528 EEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNN 587

BLAST of MS012480.1 vs. ExPASy Swiss-Prot
Match: Q9D0F1 (Kinetochore protein NDC80 homolog OS=Mus musculus OX=10090 GN=Ndc80 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 1.5e-13
Identity = 121/538 (22.49%), Postives = 242/538 (44.98%), Query Frame = 0

Query: 34  SRPSSIGMARSAAASID--IYKERAVQHSAVNAINSSLKE---LFSHSFNVTFKPSTPSS 93
           SR S +G+  S+    D     ++A     +  +   L E   ++S S      PST   
Sbjct: 66  SRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLYEFLTENGYVYSVSMKSLQAPSTKEF 125

Query: 94  LKDLTETINLLLQCLDYPPTKLEDDLPIVLKSLGYPFKINKSILKTPPAPHQWYSILALL 153
           LK        L    + P TK E+++P + K+LGYPF ++KS + T  APH W  I+A L
Sbjct: 126 LKIFAFLYGFLCPSYELPGTKCEEEVPRIFKALGYPFTLSKSSMYTVGAPHTWPHIVAAL 185

Query: 154 HWLVQVAVYNNSLANDSHSF-------------ITANHVNV-YALESYVHYIRGDDDSVE 213
            WL+     + ++   S  F             I  N + + Y  + Y  ++ G  DS E
Sbjct: 186 VWLIDCIKIDTAMKESSPLFDDGQLWGEETEDGIKHNKLFLEYTKKCYEKFMTG-ADSFE 245

Query: 214 DLDQKFMERLE---KERDNTVESL-GELKQQVEELEAKAEALRTEPSQREVLEKQQSVLE 273
           + D +   +L+   K   + +ESL  E K   E++    E    EP++   L+K ++ L+
Sbjct: 246 EEDAELQAKLKDLYKVDASKLESLEAENKALNEQIARLEEEREREPNRLMSLKKLKASLQ 305

Query: 274 EDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKMRVESQMFNPR 333
            DV+ +   ++     +  +K+     ++E+    QE + + +EN  L+  V++Q ++  
Sbjct: 306 ADVQNYKAYMSNLESHLAVLKQKSNSLDEEIGRVEQECETVKQENTRLQSIVDNQKYSVA 365

Query: 334 DVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAMECNQAMRRLKL 393
           D+ER+  E   +++ I        A +++ W+ +       + +EA   E ++  R+LK 
Sbjct: 366 DIERINHEKNELQQTINKLTKDLEAEQQQMWNEELKYARGKEAIEAQLAEYHKLARKLK- 425

Query: 394 DNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLISLRQQSSENT 453
                  L  KG+  ++  G D++    P  ++ A+ + K        L  L  +S E  
Sbjct: 426 -------LIPKGAENSK--GYDFEIKFNP--EAGANCLVKYRTQVYAPLKELLNESEEEI 485

Query: 454 AKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVRQEA--HNLDL 513
            K  ++++       H+++   Q+N+++ E+++  +R   E  + ++D+ Q+A     + 
Sbjct: 486 NKALNKKR-------HLEDTLEQLNTMKTESKN-TVRMLKEEIQKLDDLHQQAVKEAEEK 545

Query: 514 MEREAGEILKNAKLKH------QEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 541
            ++ A E+    K KH       + + ++ +E+    RE    V + ++ +  V + +
Sbjct: 546 DKKSASELESLEKHKHLLESGVNDGLSEAMDELDAVQREYQLTVKTTTEERRKVENNL 582

BLAST of MS012480.1 vs. ExPASy TrEMBL
Match: A0A6J1D5D4 (kinetochore protein NDC80 homolog OS=Momordica charantia OX=3673 GN=LOC111017757 PE=3 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.2e-305
Identity = 563/565 (99.65%), Postives = 563/565 (99.65%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS
Sbjct: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS
Sbjct: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE
Sbjct: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEA AEALRTEPSQRE
Sbjct: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEANAEALRTEPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQE KRICEENEELKM
Sbjct: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQEKKRICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME
Sbjct: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR
Sbjct: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
           QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI
Sbjct: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDMKSNLSKTAIGISEAYKNSLPAE
Sbjct: 541 SDMKSNLSKTAIGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. ExPASy TrEMBL
Match: A0A6J1JAC2 (kinetochore protein NDC80 homolog OS=Cucurbita maxima OX=3661 GN=LOC111482699 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 7.5e-258
Identity = 475/565 (84.07%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRR PK SFNPPPPPTPQFRRDSDASFASSRPSSIGMAR AAASID+YKERAVQ S
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVN INSSLKELF+HSFN+TFKP +P SLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVAVYN +LANDS SF+++N VNVY L 
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ VESL  L+ +V+ELEAKAEALR++PSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQ+VLEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QENK+ICEENEELKM
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIAGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFN RDVERMRRELQAVERDIGD EVSRN+WEEKTWD+DAT+G+KFKELEAMA E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNC QY LNAKGSTPA++MG+DYK+T+KPALDS A+DIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPALDSFAEDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAK+ESRR +I SLQSHI+E ETQVNSL+NE +DY LRCSTE+KKMVEDV+
Sbjct: 421 SLRQQSSENTAKIESRRSHILSLQSHIEEAETQVNSLKNEIRDYTLRCSTESKKMVEDVQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
            EAH++D++EREAGEILKNAKLKH+EA+KQ EEEI ICAREL+A VDSVS+Y+E VRSKI
Sbjct: 481 LEAHSIDVVEREAGEILKNAKLKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+SNLSKTA+GISEAYKNSLPAE
Sbjct: 541 SDMRSNLSKTAVGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. ExPASy TrEMBL
Match: A0A6J1E5Y9 (kinetochore protein NDC80 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430885 PE=3 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 7.0e-256
Identity = 471/565 (83.36%), Postives = 524/565 (92.74%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRR PK SFNPPPPPTPQFRRDSDASFASSRPSSIGMAR AAASID+YKERAVQ S
Sbjct: 1   MKGNGRRHPKGSFNPPPPPTPQFRRDSDASFASSRPSSIGMARPAAASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
           AVN INSSLKELF+HSFN+TFKP +P SLKDLTETI+LLLQCLDY PTKLEDDLPIVLKS
Sbjct: 61  AVNTINSSLKELFNHSFNLTFKPFSPPSLKDLTETIHLLLQCLDYHPTKLEDDLPIVLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK ILKTPPAPHQWY ILALLHWLVQVAVYN +LANDS SF+++N VNVY L 
Sbjct: 121 LGYPFKINKGILKTPPAPHQWYPILALLHWLVQVAVYNYNLANDSDSFVSSNDVNVYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SYV YIRGDDDSVE LDQ+FMERL+K RD+ VESL  L+ +V+ELEAKAEALR++PSQRE
Sbjct: 181 SYVQYIRGDDDSVEALDQEFMERLKKGRDDAVESLNGLEMEVKELEAKAEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQ+VLEEDVKKFHTMI GYSEK+QEMKKILEEKEKELEVK QENK+ICEENEELKM
Sbjct: 241 VLEKQQTVLEEDVKKFHTMIGGYSEKMQEMKKILEEKEKELEVKVQENKKICEENEELKM 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQMFN RDVERMRRELQAVERDIGD EVSRN+WEEKTWD+DAT+G+KFKELEAMA E
Sbjct: 301 RVESQMFNARDVERMRRELQAVERDIGDAEVSRNSWEEKTWDIDATVGNKFKELEAMARE 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLDNC QY LNAKGSTPA++MG+DYK+T+KP+LDS A+DIKKSSMTKLEDLI
Sbjct: 361 CNQAMRRLKLDNCHQYFLNAKGSTPADVMGVDYKTTIKPSLDSFAEDIKKSSMTKLEDLI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSEN+AK+ESRR +I SLQSHI+E ETQVNSLRNE +DY LRCSTE+KKMVED++
Sbjct: 421 SLRQQSSENSAKIESRRSHILSLQSHIEEGETQVNSLRNEIRDYTLRCSTESKKMVEDLQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
            EAH++D++EREAGE+LKNAK+KH+EA+KQ EEEI ICAREL+A VDSVS+Y+E VRSKI
Sbjct: 481 LEAHSIDVVEREAGELLKNAKVKHEEAIKQGEEEILICARELVACVDSVSQYKEFVRSKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+SNLSKTA+GISEAYKNSLPAE
Sbjct: 541 SDMRSNLSKTAVGISEAYKNSLPAE 565

BLAST of MS012480.1 vs. ExPASy TrEMBL
Match: A0A1S3AYB0 (kinetochore protein NDC80 homolog OS=Cucumis melo OX=3656 GN=LOC103484089 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.9e-240
Identity = 444/565 (78.58%), Postives = 507/565 (89.73%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRRRPK SFNPPPPPTPQFRRDSD SFASSRPSSIGM+R  +ASID+YKERAVQ S
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
            VN INSSLKELFSHSFNVTFKP +P SLKDLTETINLLLQCLDYPPTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK+ILKTPPAPHQWY ILALLHWLVQVA+YN +LANDS+     + VN+Y L 
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SY+HY+RGDDDSVE LDQ+FMERL+K RD++ ESL EL+++V+ LEAK EALR++PSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQS+LEEDVKKFHTMI GYSEK+QEMKK+LEEKEKELEVK +E  +ICEENEELK 
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQ+FNPRDVERMRRELQAVERDIGD EVSRN+WEEK+WDLDATLG+KFKELEA+AME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLD+C QY+LNAKGSTPA++MGIDYKSTVKPALDS A+DI++SSM KLE+LI
Sbjct: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAK++S+R  IASLQSHI+E ET++ SL++E +DY LRCSTE+KKMVED +
Sbjct: 421 SLRQQSSENTAKIDSKRNQIASLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
           QEAHNLDL+E EA E LKNAKL +QEA KQ EEEI +CARELL+ +DSVS+Y+  V  KI
Sbjct: 481 QEAHNLDLVEGEAEESLKNAKLANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+S LSK A GISEA+KNS+P E
Sbjct: 541 SDMESCLSKAAAGISEAFKNSMPVE 565

BLAST of MS012480.1 vs. ExPASy TrEMBL
Match: A0A5D3DHD6 (Kinetochore protein NDC80-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00670 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.9e-240
Identity = 444/565 (78.58%), Postives = 507/565 (89.73%), Query Frame = 0

Query: 1   MRGAGRRRPKESFNPPPPPTPQFRRDSDASFASSRPSSIGMARSAAASIDIYKERAVQHS 60
           M+G GRRRPK SFNPPPPPTPQFRRDSD SFASSRPSSIGM+R  +ASID+YKERAVQ S
Sbjct: 1   MKGTGRRRPKASFNPPPPPTPQFRRDSDVSFASSRPSSIGMSRPPSASIDMYKERAVQIS 60

Query: 61  AVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYPPTKLEDDLPIVLKS 120
            VN INSSLKELFSHSFNVTFKP +P SLKDLTETINLLLQCLDYPPTKLEDDLPI+LKS
Sbjct: 61  TVNTINSSLKELFSHSFNVTFKPFSPPSLKDLTETINLLLQCLDYPPTKLEDDLPILLKS 120

Query: 121 LGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHSFITANHVNVYALE 180
           LGYPFKINK+ILKTPPAPHQWY ILALLHWLVQVA+YN +LANDS+     + VN+Y L 
Sbjct: 121 LGYPFKINKNILKTPPAPHQWYQILALLHWLVQVALYNYNLANDSNPVFMGHDVNIYVLN 180

Query: 181 SYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEAKAEALRTEPSQRE 240
           SY+HY+RGDDDSVE LDQ+FMERL+K RD++ ESL EL+++V+ LEAK EALR++PSQRE
Sbjct: 181 SYLHYMRGDDDSVEALDQEFMERLKKTRDDSCESLKELEEEVKVLEAKVEALRSDPSQRE 240

Query: 241 VLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQENKRICEENEELKM 300
           VLEKQQS+LEEDVKKFHTMI GYSEK+QEMKK+LEEKEKELEVK +E  +ICEENEELK 
Sbjct: 241 VLEKQQSLLEEDVKKFHTMIAGYSEKMQEMKKLLEEKEKELEVKMEERNKICEENEELKK 300

Query: 301 RVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATLGHKFKELEAMAME 360
           RVESQ+FNPRDVERMRRELQAVERDIGD EVSRN+WEEK+WDLDATLG+KFKELEA+AME
Sbjct: 301 RVESQLFNPRDVERMRRELQAVERDIGDAEVSRNSWEEKSWDLDATLGNKFKELEAIAME 360

Query: 361 CNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADDIKKSSMTKLEDLI 420
           CNQAMRRLKLD+C QY+LNAKGSTPA++MGIDYKSTVKPALDS A+DI++SSM KLE+LI
Sbjct: 361 CNQAMRRLKLDSCHQYVLNAKGSTPADVMGIDYKSTVKPALDSFAEDIRRSSMMKLEELI 420

Query: 421 SLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLRCSTEAKKMVEDVR 480
           SLRQQSSENTAK++S+R  IASLQSHI+E ET++ SL++E +DY LRCSTE+KKMVED +
Sbjct: 421 SLRQQSSENTAKIDSKRNQIASLQSHINEHETKLISLKDEMRDYILRCSTESKKMVEDFQ 480

Query: 481 QEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVDSVSKYQESVRSKI 540
           QEAHNLDL+E EA E LKNAKL +QEA KQ EEEI +CARELL+ +DSVS+Y+  V  KI
Sbjct: 481 QEAHNLDLVEGEAEESLKNAKLANQEATKQEEEEIHMCARELLSCLDSVSQYKAVVSCKI 540

Query: 541 SDMKSNLSKTAIGISEAYKNSLPAE 566
           SDM+S LSK A GISEA+KNS+P E
Sbjct: 541 SDMESCLSKAAAGISEAFKNSMPVE 565

BLAST of MS012480.1 vs. TAIR 10
Match: AT3G54630.1 (CONTAINS InterPro DOMAIN/s: Kinetochore protein Ndc80 (InterPro:IPR005550); Has 24780 Blast hits to 15608 proteins in 1321 species: Archae - 545; Bacteria - 2969; Metazoa - 12597; Fungi - 2181; Plants - 1581; Viruses - 39; Other Eukaryotes - 4868 (source: NCBI BLink). )

HSP 1 Score: 441.8 bits (1135), Expect = 8.2e-124
Identity = 258/572 (45.10%), Postives = 388/572 (67.83%), Query Frame = 0

Query: 1   MRG--AGRRRPKESFN---PPPPPTPQFR------RDSDASFASSRPSSIGMARSAAASI 60
           MRG  AG+RR    F    PPPPP+ + +      RDSDASFASSRPSSIG+    A+  
Sbjct: 1   MRGGAAGKRRTTVGFGGAPPPPPPSIEQQRHLFNSRDSDASFASSRPSSIGLGGRGASD- 60

Query: 61  DIYKERAVQHSAVNAINSSLKELFSHSFNVTFKPSTPSSLKDLTETINLLLQCLDYP--P 120
               +R+     +NA       L +H+F ++ + +   S+KD++ET+  LL  LDYP   
Sbjct: 61  ----DRSSMIRFINAF------LSTHNFPISIRGNPVPSVKDISETLKFLLSALDYPCDS 120

Query: 121 TKLEDDLPIVLKSLGYPFKINKSILKTPPAPHQWYSILALLHWLVQVAVYNNSLANDSHS 180
            K ++DL   LKS   PFKI KS LK P  PH W ++LA++HWL ++A ++  L ++S S
Sbjct: 121 IKWDEDLVFFLKSQKCPFKITKSSLKAPNTPHNWPTVLAVVHWLAELARFHQHLVSNSTS 180

Query: 181 FITANHVNVYALESYVHYIRGDDDSVEDLDQKFMERLEKERDNTVESLGELKQQVEELEA 240
               N +N +A++S+ H+IRG+DD V DLD +F+ +LE E+ +  E++   ++   ELEA
Sbjct: 181 VPEDNSMNFFAIQSFGHFIRGEDDKVNDLDSQFLGKLEAEKTSVAETISGCEKISGELEA 240

Query: 241 KAEALRTEPSQREVLEKQQSVLEEDVKKFHTMITGYSEKVQEMKKILEEKEKELEVKAQE 300
           K E+LR  PS++E LEK ++ LE DV KF T++  Y+++   M+K++EEK KEL+ K +E
Sbjct: 241 KLESLRKGPSKKESLEKVKADLENDVNKFRTIVVEYTDRNPAMEKVVEEKAKELKAKEEE 300

Query: 301 NKRICEENEELKMRVESQMFNPRDVERMRRELQAVERDIGDGEVSRNAWEEKTWDLDATL 360
            +RI  EN+ELK  VE Q F+  DV RMRRELQAVERD+ D EV+R+ W++K W+L++ +
Sbjct: 301 RERISVENKELKKSVELQNFSAADVNRMRRELQAVERDVADAEVARDGWDQKAWELNSQI 360

Query: 361 GHKFKELEAMAMECNQAMRRLKLDNCRQYILNAKGSTPAELMGIDYKSTVKPALDSLADD 420
            ++F +++ +A++CNQA+RRLKLD   Q+ +N +G TPA +MG+DYKS VKPAL SL D 
Sbjct: 361 RNQFHQIQTLAIDCNQALRRLKLD--IQFAVNERGETPAAVMGVDYKSVVKPALCSLCDG 420

Query: 421 IKKSSMTKLEDLISLRQQSSENTAKMESRRKNIASLQSHIDEIETQVNSLRNETQDYRLR 480
           IK SS  K+E+L++L+   SE  +K+ES+R  + S+Q  I+++E ++  ++ ETQ+   +
Sbjct: 421 IKGSSAEKVEELVTLQHHKSEMASKIESKRSLLGSIQLQINDLEEKMKLVKKETQELSTK 480

Query: 481 CSTEAKKMVEDVRQEAHNLDLMEREAGEILKNAKLKHQEAVKQSEEEIQICARELLAWVD 540
           C  EAK +VE V+ EA NL+++E+EA E +K ++L+ QEAVK+SEEE+Q CA +L A +D
Sbjct: 481 CDLEAKTLVESVKAEALNLEVVEKEAAEFVKASELRLQEAVKESEEEVQACAAQLFALID 540

Query: 541 SVSKYQESVRSKISDMKSNLSKTAIGISEAYK 560
           S+SK +E + SKIS++K+ ++ TA  +SE YK
Sbjct: 541 SISKQKEYMDSKISEIKTGVADTASAVSEIYK 559