MS012289.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS012289.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	phospholipase A1-Ibeta2, chloroplastic
Location	scaffold797: 1235663 .. 1237138 (-)
Sequence length	1476
RNA-Seq Expression	MS012289.1
Synteny	MS012289.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAATCGGCTCCACGATTCCGGCACAGAATCTCTCCCGGTTTCAACTCCGGCGTTCCAGTTTCAAGTACGAGAAGTCGCCGTTGAATCCGACGGCGAAGATTTCTTCCTTCAAACCAGCGGCGGGTTCCTGCGACGTGAATCGCCGGCACCTGTCAAATCTCGACAAATTGCTGCAGAAGACCGAGCCGCCGGCGAAAACCGAACCGCAGCCGGTTCACAAGGATCCGAACAAGGGGTTGCCGATTAAGGGGAAGGGACTTCTGGAAGGGCTGAATCTGTCCCGAATCTGGCCGGAGATGAAGGCGGCGGAGGAAATGTCGCCGAGGAATCTGAACCGGCTGCAGCGGCTGCTGTCGAAGACGGTGGAGTATTCGCCGAGAAACGCCCTCGGCGGGCGGTGGCGCGAGTACCACGGCTGCAACGACTGGGCCGGCCTCCTCGACCCGCTCGACGAGAATCTCCGCCGCGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCGTACCACGCCTTCCACTCAAACCCCGCCACGTCACCCGACGAGGCCCCACTCCCCCGCCACGTCTCCCTCCCGGACAGGTCCTACAAAGTCACCAAAAGTCTCTACGCCACGTCATCCGTCGGCCTACCCGATTGGGTCGACCACGTGGCCCCGGACCTCGGGTGGATGACTCAGCGGTCCAGCTGGATCGGATACGTCGCCGTTTGCGACGACCGCCGCGAAATTGCCCGGATGGGGCGGCGGGACATCGTCATCTCCCTCCGCGGCACCGCCACCTGCCTCGAATGGGCTGAGAACGTCAGGGCCCACTTAATCGACATCGACGCCGACGGAGATCCGAAAGTCGAGTGCGGATTTCTGAGCCTGTACAGAACCGGCGGCGCCCACGTGAAGAGCCTTGCGGAGTCGGTGGTGGAGGAAATCCGGCGATTAACAGAGCAGTACAGAGGAGAGACGCTGAGCATAACGGTGACAGGGCACAGCCTGGGGGCGGCATTGGCTCTGCTGGTGGCGGACGAGATCAGTGTGTGCTCGCCGGAAATCCCTCCAGTGGCGGTGTTCTCGTTCGGGGGGCCGCGCGTGGGGAACAAGGCGTTCGCGAACAGAGTAAAATCGCGGAACGTGAAGGTTCTACGGATGGTGAACTCGCAGGACGTGATAACGCAGGTGCCGGCGGTGAGGTACTCGCACGTGGGGACGGAGCTGCGTGTGGAGACGAAGATGTCGCCGTACCTGAAACCGAACGCCGACGTGGCCTGCTGCCACGATCTGGAGGCGTATTTGCATCTGGTGGACGGATTCTTGTCGTCGAAGTGCCCGTTCCGGCCGAACGCGAAACGGAGTTTGGCCCGATTGGTGCAGGATCAGAAGGGGAACTTGAAGAAATTGTACATGAGCAAGGTCAAGGATTTGGGCCGCCTCAACCCGGAGTTTCACGCCGCCGGACCCGGATGTTTGCCCAGCCCGTCG

mRNA sequence

ATGCAAATCGGCTCCACGATTCCGGCACAGAATCTCTCCCGGTTTCAACTCCGGCGTTCCAGTTTCAAGTACGAGAAGTCGCCGTTGAATCCGACGGCGAAGATTTCTTCCTTCAAACCAGCGGCGGGTTCCTGCGACGTGAATCGCCGGCACCTGTCAAATCTCGACAAATTGCTGCAGAAGACCGAGCCGCCGGCGAAAACCGAACCGCAGCCGGTTCACAAGGATCCGAACAAGGGGTTGCCGATTAAGGGGAAGGGACTTCTGGAAGGGCTGAATCTGTCCCGAATCTGGCCGGAGATGAAGGCGGCGGAGGAAATGTCGCCGAGGAATCTGAACCGGCTGCAGCGGCTGCTGTCGAAGACGGTGGAGTATTCGCCGAGAAACGCCCTCGGCGGGCGGTGGCGCGAGTACCACGGCTGCAACGACTGGGCCGGCCTCCTCGACCCGCTCGACGAGAATCTCCGCCGCGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCGTACCACGCCTTCCACTCAAACCCCGCCACGTCACCCGACGAGGCCCCACTCCCCCGCCACGTCTCCCTCCCGGACAGGTCCTACAAAGTCACCAAAAGTCTCTACGCCACGTCATCCGTCGGCCTACCCGATTGGGTCGACCACGTGGCCCCGGACCTCGGGTGGATGACTCAGCGGTCCAGCTGGATCGGATACGTCGCCGTTTGCGACGACCGCCGCGAAATTGCCCGGATGGGGCGGCGGGACATCGTCATCTCCCTCCGCGGCACCGCCACCTGCCTCGAATGGGCTGAGAACGTCAGGGCCCACTTAATCGACATCGACGCCGACGGAGATCCGAAAGTCGAGTGCGGATTTCTGAGCCTGTACAGAACCGGCGGCGCCCACGTGAAGAGCCTTGCGGAGTCGGTGGTGGAGGAAATCCGGCGATTAACAGAGCAGTACAGAGGAGAGACGCTGAGCATAACGGTGACAGGGCACAGCCTGGGGGCGGCATTGGCTCTGCTGGTGGCGGACGAGATCAGTGTGTGCTCGCCGGAAATCCCTCCAGTGGCGGTGTTCTCGTTCGGGGGGCCGCGCGTGGGGAACAAGGCGTTCGCGAACAGAGTAAAATCGCGGAACGTGAAGGTTCTACGGATGGTGAACTCGCAGGACGTGATAACGCAGGTGCCGGCGGTGAGGTACTCGCACGTGGGGACGGAGCTGCGTGTGGAGACGAAGATGTCGCCGTACCTGAAACCGAACGCCGACGTGGCCTGCTGCCACGATCTGGAGGCGTATTTGCATCTGGTGGACGGATTCTTGTCGTCGAAGTGCCCGTTCCGGCCGAACGCGAAACGGAGTTTGGCCCGATTGGTGCAGGATCAGAAGGGGAACTTGAAGAAATTGTACATGAGCAAGGTCAAGGATTTGGGCCGCCTCAACCCGGAGTTTCACGCCGCCGGACCCGGATGTTTGCCCAGCCCGTCG

Coding sequence (CDS)

ATGCAAATCGGCTCCACGATTCCGGCACAGAATCTCTCCCGGTTTCAACTCCGGCGTTCCAGTTTCAAGTACGAGAAGTCGCCGTTGAATCCGACGGCGAAGATTTCTTCCTTCAAACCAGCGGCGGGTTCCTGCGACGTGAATCGCCGGCACCTGTCAAATCTCGACAAATTGCTGCAGAAGACCGAGCCGCCGGCGAAAACCGAACCGCAGCCGGTTCACAAGGATCCGAACAAGGGGTTGCCGATTAAGGGGAAGGGACTTCTGGAAGGGCTGAATCTGTCCCGAATCTGGCCGGAGATGAAGGCGGCGGAGGAAATGTCGCCGAGGAATCTGAACCGGCTGCAGCGGCTGCTGTCGAAGACGGTGGAGTATTCGCCGAGAAACGCCCTCGGCGGGCGGTGGCGCGAGTACCACGGCTGCAACGACTGGGCCGGCCTCCTCGACCCGCTCGACGAGAATCTCCGCCGCGAAGTCGTCCGGTACGGCGAGTTCGTACAAGCCGCGTACCACGCCTTCCACTCAAACCCCGCCACGTCACCCGACGAGGCCCCACTCCCCCGCCACGTCTCCCTCCCGGACAGGTCCTACAAAGTCACCAAAAGTCTCTACGCCACGTCATCCGTCGGCCTACCCGATTGGGTCGACCACGTGGCCCCGGACCTCGGGTGGATGACTCAGCGGTCCAGCTGGATCGGATACGTCGCCGTTTGCGACGACCGCCGCGAAATTGCCCGGATGGGGCGGCGGGACATCGTCATCTCCCTCCGCGGCACCGCCACCTGCCTCGAATGGGCTGAGAACGTCAGGGCCCACTTAATCGACATCGACGCCGACGGAGATCCGAAAGTCGAGTGCGGATTTCTGAGCCTGTACAGAACCGGCGGCGCCCACGTGAAGAGCCTTGCGGAGTCGGTGGTGGAGGAAATCCGGCGATTAACAGAGCAGTACAGAGGAGAGACGCTGAGCATAACGGTGACAGGGCACAGCCTGGGGGCGGCATTGGCTCTGCTGGTGGCGGACGAGATCAGTGTGTGCTCGCCGGAAATCCCTCCAGTGGCGGTGTTCTCGTTCGGGGGGCCGCGCGTGGGGAACAAGGCGTTCGCGAACAGAGTAAAATCGCGGAACGTGAAGGTTCTACGGATGGTGAACTCGCAGGACGTGATAACGCAGGTGCCGGCGGTGAGGTACTCGCACGTGGGGACGGAGCTGCGTGTGGAGACGAAGATGTCGCCGTACCTGAAACCGAACGCCGACGTGGCCTGCTGCCACGATCTGGAGGCGTATTTGCATCTGGTGGACGGATTCTTGTCGTCGAAGTGCCCGTTCCGGCCGAACGCGAAACGGAGTTTGGCCCGATTGGTGCAGGATCAGAAGGGGAACTTGAAGAAATTGTACATGAGCAAGGTCAAGGATTTGGGCCGCCTCAACCCGGAGTTTCACGCCGCCGGACCCGGATGTTTGCCCAGCCCGTCG

Protein sequence

MQIGSTIPAQNLSRFQLRRSSFKYEKSPLNPTAKISSFKPAAGSCDVNRRHLSNLDKLLQKTEPPAKTEPQPVHKDPNKGLPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRLLSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDADGDPKVECGFLSLYRTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAVRYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKVKDLGRLNPEFHAAGPGCLPSPS

Homology

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BLAST of MS012289.1 vs. NCBI nr
Match: XP_038887009.1 (phospholipase A1-Ibeta2, chloroplastic [Benincasa hispida])

HSP 1 Score: 870.2 bits (2247), Expect = 8.7e-249
Identity = 429/501 (85.63%), Postives = 460/501 (91.82%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPT-AKISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIPAQNLS+FQ+RRSSFKY+ SPLNP+ A+ISSFKPA  + D  RRHL+NLDKLL+K
Sbjct: 4   ISSTIPAQNLSQFQVRRSSFKYQMSPLNPSAAQISSFKPATSTVDGARRHLANLDKLLKK 63

Query: 63  TEPPAKTEPQPVHKDPNKG--LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRLL 122
           T+PP K +P+PVHKDPNKG  +PIKGKGLLEGLNL+RIWPE+KA EEMSPRNLNRLQRLL
Sbjct: 64  TDPPVKVDPEPVHKDPNKGSVMPIKGKGLLEGLNLARIWPEVKAVEEMSPRNLNRLQRLL 123

Query: 123 SKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT 182
           SKT+EYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT
Sbjct: 124 SKTMEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT 183

Query: 183 SPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCD 242
           SP+EAPLPRHV+LPDRSYKVTKSLYATSSVGLP WVD VAPDLGWMTQRSSW+GYVAVCD
Sbjct: 184 SPNEAPLPRHVALPDRSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWVGYVAVCD 243

Query: 243 DRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD--------GDPKVECGFLSL 302
           DRREIARMGRRDIVI+LRGTATCLEWAENVRA LI+I AD        GDPKVECGFLSL
Sbjct: 244 DRREIARMGRRDIVIALRGTATCLEWAENVRAQLINIPADADAAAEDGGDPKVECGFLSL 303

Query: 303 YRTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIP 362
           Y+T GAHVKSL+ESVVEEIRRLTE Y+GE LSITVTGHSLGAALA+LVADEISVCSPE+P
Sbjct: 304 YKTAGAHVKSLSESVVEEIRRLTEMYKGERLSITVTGHSLGAALAVLVADEISVCSPEVP 363

Query: 363 PVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAVRYSHVGTELRVETKMS 422
           PVAVFSFGGPRVGNK FANR+KSRNVKVLR+VNSQDVITQVP V YSHVGTELRVETKMS
Sbjct: 364 PVAVFSFGGPRVGNKIFANRIKSRNVKVLRIVNSQDVITQVPPVTYSHVGTELRVETKMS 423

Query: 423 PYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKVK 482
           P+LKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KVK
Sbjct: 424 PFLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLVRLVQDQRGNVKKLYMRKVK 483

Query: 483 DLGRLNPEFHAAGPGCLPSPS 493
           DLG  NPE   A  GCLPSPS
Sbjct: 484 DLG-FNPELQTA--GCLPSPS 501

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BLAST of MS012289.1 vs. NCBI nr
Match: XP_008465868.2 (PREDICTED: phospholipase A1-Ibeta2, chloroplastic [Cucumis melo] >TYK31086.1 phospholipase A1-Ibeta2 [Cucumis melo var. makuwa])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 422/502 (84.06%), Postives = 453/502 (90.24%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS F LRRSSFKY+ SPLNP+A KISSF+ AA +  V RRHL+NLDKLL K
Sbjct: 5   INSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLNK 64

Query: 63  TEPPAKTEPQPVHKDPNKG---LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRL 122
           T+PP K +PQPVHKDPNKG   +PIKGKGLLEGLNLSR+WPE+KA EEMSPRNLNRLQRL
Sbjct: 65  TDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQRL 124

Query: 123 LSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 182
           LSKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 
Sbjct: 125 LSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPT 184

Query: 183 TSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVC 242
           TSP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVC
Sbjct: 185 TSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVC 244

Query: 243 DDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLY 302
           DDRREIARMGRRDIVI+LRGTATCLEWAENVRA LIDI AD      GDPKVECGFLSLY
Sbjct: 245 DDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVDTKDCGDPKVECGFLSLY 304

Query: 303 RTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPP 362
           +T GAHV+SL+ESVVEEIRRLTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PP
Sbjct: 305 KTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAEVPP 364

Query: 363 VAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKM 422
           VAVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQDVITQVP   + YSHVGTELRVETKM
Sbjct: 365 VAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVETKM 424

Query: 423 SPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKV 482
           SP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KV
Sbjct: 425 SPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKV 484

Query: 483 KDLGRLNPEFHAAGPGCLPSPS 493
           KDLG  NPE      GCLPSPS
Sbjct: 485 KDLG-FNPELQTV--GCLPSPS 503

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BLAST of MS012289.1 vs. NCBI nr
Match: KAA0038492.1 (phospholipase A1-Ibeta2 [Cucumis melo var. makuwa])

HSP 1 Score: 851.7 bits (2199), Expect = 3.2e-243
Identity = 421/502 (83.86%), Postives = 453/502 (90.24%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS F LRRSSFKY+ SPLNP+A KISSF+ AA +  V RRHL+NLDKLL K
Sbjct: 5   INSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLNK 64

Query: 63  TEPPAKTEPQPVHKDPNKG---LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRL 122
           T+PP K +PQPVHKDPNKG   +PIKGKGLLEGLNLSR+WPE+KA EEMSPRNLNRLQRL
Sbjct: 65  TDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQRL 124

Query: 123 LSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 182
           LSKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 
Sbjct: 125 LSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPT 184

Query: 183 TSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVC 242
           TSP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVC
Sbjct: 185 TSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVC 244

Query: 243 DDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLY 302
           DDRREIARMGRRDIVI+LRGTATCLEWAENVRA LIDI AD      GDPKVECGFLSLY
Sbjct: 245 DDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVDTKDCGDPKVECGFLSLY 304

Query: 303 RTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPP 362
           +T GAHV+SL+ESVVEEIR+LTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PP
Sbjct: 305 KTAGAHVRSLSESVVEEIRQLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAEVPP 364

Query: 363 VAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKM 422
           VAVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQDVITQVP   + YSHVGTELRVETKM
Sbjct: 365 VAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVETKM 424

Query: 423 SPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKV 482
           SP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KV
Sbjct: 425 SPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKV 484

Query: 483 KDLGRLNPEFHAAGPGCLPSPS 493
           KDLG  NPE      GCLPSPS
Sbjct: 485 KDLG-FNPELQTV--GCLPSPS 503

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BLAST of MS012289.1 vs. NCBI nr
Match: XP_004148351.1 (phospholipase A1-Ibeta2, chloroplastic [Cucumis sativus] >KGN60475.1 hypothetical protein Csa_001794 [Cucumis sativus])

HSP 1 Score: 841.6 bits (2173), Expect = 3.3e-240
Identity = 415/501 (82.83%), Postives = 452/501 (90.22%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS+F LRRSSFKY+ SPLNP+A KI+SF+ AA +  V RRHL+NLDKLL K
Sbjct: 4   INSTIPPQNLSQFHLRRSSFKYQISPLNPSAPKITSFRSAAVAVHVTRRHLANLDKLLNK 63

Query: 63  TEPPAKTEPQPVHKDPNK--GLPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRLL 122
           T+PP K +PQPVHKDPNK   +PIKGKGLLE LNL+R+WPE+KAAE+MSPRNLNRLQRLL
Sbjct: 64  TDPPLKVDPQPVHKDPNKVSPMPIKGKGLLESLNLARLWPEVKAAEDMSPRNLNRLQRLL 123

Query: 123 SKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT 182
           SKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP T
Sbjct: 124 SKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPTT 183

Query: 183 SPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCD 242
           SP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVCD
Sbjct: 184 SPNEPPLPRHVALPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVCD 243

Query: 243 DRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLYR 302
           DRREIARMGRRDIVI+LRGTATCLEWAENVRA L ++ AD      GDPKVECGFLSLY+
Sbjct: 244 DRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNVPADVDTKDGGDPKVECGFLSLYK 303

Query: 303 TGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPV 362
           T GAHVKSL+ESVVEEIRRLTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PPV
Sbjct: 304 TAGAHVKSLSESVVEEIRRLTELYKGETLSITVTGHSLGAALAILVADEISVCSAEVPPV 363

Query: 363 AVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKMS 422
           AVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQD+ITQVP   + YSHVGTELRVETKMS
Sbjct: 364 AVFSFGGPRVGNKIFADRIKSRNVKVLRIVNSQDLITQVPPNPMTYSHVGTELRVETKMS 423

Query: 423 PYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKVK 482
           P+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KVK
Sbjct: 424 PFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKVK 483

Query: 483 DLGRLNPEFHAAGPGCLPSPS 493
           DLG  NPE      GCLPSPS
Sbjct: 484 DLG-FNPELQTV--GCLPSPS 501

[Close]

BLAST of MS012289.1 vs. NCBI nr
Match: XP_022931784.1 (phospholipase A1-Ibeta2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 840.5 bits (2170), Expect = 7.4e-240
Identity = 417/496 (84.07%), Postives = 449/496 (90.52%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPT-AKISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIPAQNLSRFQ+RRSSFKY+ SPLNP+ AKIS F+PA  + D  RRHL+NLDKLL+K
Sbjct: 4   INSTIPAQNLSRFQVRRSSFKYQMSPLNPSAAKISYFRPATAAVDGTRRHLANLDKLLKK 63

Query: 63  TEPPAKTEPQPVHKDPNKG--LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRLL 122
           T+ PAK EP+PV KDPNKG  +PIKGKGLLEGLNL+RIWPEM+AAE+MSPRNLNRLQRLL
Sbjct: 64  TDTPAKVEPEPVFKDPNKGSVMPIKGKGLLEGLNLARIWPEMRAAEDMSPRNLNRLQRLL 123

Query: 123 SKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT 182
           SKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQ+AYH+FHSNPA 
Sbjct: 124 SKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQSAYHSFHSNPAM 183

Query: 183 SPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCD 242
           S +EAPLPRHV+LPD+SYKVTKSLYATSSVGLP WVD VAPDLGWMTQRSSW+GYVAVCD
Sbjct: 184 SLNEAPLPRHVALPDKSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWMGYVAVCD 243

Query: 243 DRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD---GDPKVECGFLSLYRTGG 302
           DRREIARMGRRDIVI+LRGTATCLEWAENVRA L +I  D    DPKVECGFLSLY+T G
Sbjct: 244 DRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNIPTDTNSSDPKVECGFLSLYKTAG 303

Query: 303 AHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPVAVF 362
           AHVKSL+ESVVEEIRRL E Y+GETLSI+VTGHSLGAALALLVADEISVCSP +PPVAVF
Sbjct: 304 AHVKSLSESVVEEIRRLMEIYKGETLSISVTGHSLGAALALLVADEISVCSPTVPPVAVF 363

Query: 363 SFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAVRYSHVGTELRVETKMSPYLKP 422
           SFGGPRVGN AFANR+ SRNVKVLR+VNSQD+ITQVP V YSHVGTELRVETKMSPYLKP
Sbjct: 364 SFGGPRVGNTAFANRITSRNVKVLRIVNSQDLITQVPPVTYSHVGTELRVETKMSPYLKP 423

Query: 423 NADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKVKDLGRL 482
           NADVACCHDLEAYLHLVDGFLSSKCPFR NAKRSL RLV DQ+GN+KKLYM KVKDL  L
Sbjct: 424 NADVACCHDLEAYLHLVDGFLSSKCPFRANAKRSLVRLVHDQRGNMKKLYMRKVKDLS-L 483

Query: 483 NPEFHAAGPGCLPSPS 493
           NPE      GCLPSPS
Sbjct: 484 NPELQML--GCLPSPS 496

[Close]

BLAST of MS012289.1 vs. ExPASy Swiss-Prot
Match: O23522 (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 549.3 bits (1414), Expect = 4.5e-155
Identity = 302/524 (57.63%), Postives = 355/524 (67.75%), Query Frame = 0


Query: 8   PAQNLSRFQLRRSSFKYEKSPLNPTAKISSFKPAAGSCDVNRRHLSNLDKLLQKTEPPAK 67
           P  ++   + RR +     S L PT K  S  PA      N+ HL NL+ +L+ +     
Sbjct: 6   PNADIFHAKRRRFTCNTHTSTLIPT-KPLSVSPAR---KTNKEHLRNLENVLRTSSNSID 65

Query: 68  TEPQPVHKDPNKGLPIKGKGLLEGLNLSRIWPEMKAA-EEMSPRNLNRLQRLLSK-TVEY 127
                  +            LL GLNL+RIWP+MKAA +EMSP+NL RLQRLLSK + E 
Sbjct: 66  HIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEER 125

Query: 128 SPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSPDEAP 187
           SP++ LG +WRE HG N+WAGLLDPLDENLRRE+VRYGEFVQAAYHAFHS+P  S     
Sbjct: 126 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGS----- 185

Query: 188 LPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIA 247
            PRHV+LPD S+KVTKSLYATSSV LP W+D VAPDL WMT+++SW+GYVAVCDD REI 
Sbjct: 186 -PRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIR 245

Query: 248 RMGRRDIVISLRGTATCLEWAENVRAHLI-------DIDADGDPKVECGFLSLYRTGGAH 307
           RMGRR+IVI+LRGTAT LEW+EN R +L+       D      PKVECGF SLY TG  H
Sbjct: 246 RMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTTGDQH 305

Query: 308 VKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPVAVFSF 367
             SLAES+V EI RL E Y GE LSI+VTGHSLGAA+ALL AD+I+   P  PPVAVFSF
Sbjct: 306 APSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFSF 365

Query: 368 GGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV----------------------- 427
           GGPRVGN+ FA+R+ S+ VKVLR+VNSQDV+T+VP +                       
Sbjct: 366 GGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIMEM 425

Query: 428 -------RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAK 487
                   YSHVG ELRV+ KMSPYLKPNADVACCHDLEAYLHLVDGFL+S CPFR NAK
Sbjct: 426 VERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANAK 485

Query: 488 RSLARLVQDQKGNLKKLYMSKVKDLGRLNPEFHAAGPGCLPSPS 493
           RSL +L+ +Q+ N+K LY  K     RLN  FH  G   LPSPS
Sbjct: 486 RSLRKLLDEQRSNVKVLYTGKSL---RLNRTFHDNG-DVLPSPS 515

[Close]

BLAST of MS012289.1 vs. ExPASy Swiss-Prot
Match: Q948R1 (Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 PE=1 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.8e-88
Identity = 179/381 (46.98%), Postives = 233/381 (61.15%), Query Frame = 0


Query: 124 EYSPRNA-----LGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 183
           E+SP  +     LG RW EY G  +W GLLDPLD+NLRRE++RYG+FV++AY AF  +P+
Sbjct: 74  EFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPS 133

Query: 184 TSP------DEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWI 243
           +          + L     LP+  Y++TK+L ATS + LP W++  AP   WM  +SSWI
Sbjct: 134 SPTYGTCRFPRSTLLERSGLPNSGYRLTKNLRATSGINLPRWIEK-AP--SWMATQSSWI 193

Query: 244 GYVAVCDDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDI----------DADGDP 303
           GYVAVC D+ EI+R+GRRD+VIS RGTATCLEW EN+RA L  +           ++  P
Sbjct: 194 GYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGP 253

Query: 304 KVECGFLSLYRTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADE 363
            VE GFLSLY +G   V SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A +
Sbjct: 254 MVESGFLSLYTSG---VHSLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYD 313

Query: 364 ISVCSPEIPPVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV------- 423
           I       P V V SFGGPRVGN+ F   ++ +  KVLR+VNS DVIT+VP V       
Sbjct: 314 IKTTFKRAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQ 373

Query: 424 -----------------------RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHL 454
                                   Y+ +G ELR+ ++ SP+L  + +VA CH+L+ YLHL
Sbjct: 374 DNVKMTASIMPSWIQRRVEETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLHL 433

[Close]

BLAST of MS012289.1 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.3e-65
Identity = 144/347 (41.50%), Postives = 196/347 (56.48%), Query Frame = 0


Query: 135 WREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATS--------PDEAPL 194
           WRE  GCN+W G LDP++ +LRRE++RYGEF QA Y +F  +P +         P +  L
Sbjct: 91  WREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFL 150

Query: 195 PRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIAR 254
              + L  + Y +T+ LYATS++ LP++         W +Q ++W+G+VAV  D  E++R
Sbjct: 151 NLDLHL-HKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVAVATDEEEVSR 210

Query: 255 MGRRDIVISLRGTATCLEWAENVRAHLIDIDADGDP--KVECGFLSLYRTGGAHVK---- 314
           +GRRDIVI+ RGT T LEW  +++  L   +   DP  K+E GF  LY       K    
Sbjct: 211 LGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFSSF 270

Query: 315 SLAESVVEEIRRLTEQY----RGETLSITVTGHSLGAALALLVADEISVCS-PEIP---- 374
           S  E V+ E++RL E Y     G   SITVTGHSLGA+LAL+ A +I+  +   +P    
Sbjct: 271 SAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNY 330

Query: 375 --PVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV-------------- 434
             P+ VFSF GPRVGN  F  R     VKVLR+VN  D +  VP +              
Sbjct: 331 KIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEE 390

Query: 435 ------RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 437
                  Y+HVG EL ++ K SP+LKP  D+ C H+LEA LHLVDG+
Sbjct: 391 KTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGY 435

[Close]

BLAST of MS012289.1 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 233.0 bits (593), Expect = 7.1e-60
Identity = 137/346 (39.60%), Postives = 196/346 (56.65%), Query Frame = 0


Query: 135 WREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSP------DEAPLPR 194
           WR+  G +DWAGL+DP+D  LR E++RYGE  QA Y AF  +PA+               
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 195 HVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIARMG 254
            + + D  Y+V + LYATS++ LP++         W ++ ++W+GYVAV DD     R+G
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSRNRLG 224

Query: 255 RRDIVISLRGTATCLEWAENVRAHLIDIDAD----GDP--KVECGFLSLYRTGGAHVK-- 314
           RRDI I+ RGT T LEW  +++ +L  +  +     DP  KVE GFL LY       K  
Sbjct: 225 RRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFA 284

Query: 315 --SLAESVVEEIRRLTEQYRGE---TLSITVTGHSLGAALALLVADEISVC------SPE 374
             S  E ++ E++RL E++  +    LSITVTGHSLG ALA+L A +I+          +
Sbjct: 285 RFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGK 344

Query: 375 IPPVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV-------------- 434
           + PV V ++GGPRVGN  F  R++   VKV+R+VN  DV+ + P +              
Sbjct: 345 VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIA 404

Query: 435 -----RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 437
                 YSHVG EL ++ + SP+LKP+ DV+  H+LEA LHL+DG+
Sbjct: 405 EGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGY 449

[Close]

BLAST of MS012289.1 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 232.6 bits (592), Expect = 9.3e-60
Identity = 142/376 (37.77%), Postives = 200/376 (53.19%), Query Frame = 0


Query: 106 EMSPRNLNRLQRLLSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEF 165
           E     L   + +  +  E      L   WR+  G +DWAGL+DP+D  LR E++RYGE 
Sbjct: 60  EQESYGLTTAEDIRRRDGEAKESKRLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEM 119

Query: 166 VQAAYHAFHSNP------ATSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVA 225
            QA Y AF  +P      +       L   + + D  Y+V + LYATS++ LP++     
Sbjct: 120 AQACYDAFDFDPFSRYCGSCRFTRRHLFDSLGIIDSGYEVARYLYATSNINLPNFFSKSR 179

Query: 226 PDLGWMTQRSSWIGYVAVCDDRREI-ARMGRRDIVISLRGTATCLEWAENVRAHLIDIDA 285
               W ++ ++W+GYVAV DD      R+GRRDI I+ RGT T LEW  +++  L  +  
Sbjct: 180 WSKVW-SKNANWMGYVAVSDDNEATRCRLGRRDIAIAWRGTVTRLEWIADLKDFLKPVSG 239

Query: 286 DG----DP--KVECGFLSLYRTGGAHVK----SLAESVVEEIRRLTEQY---RGETLSIT 345
           +G    DP  K E GFL LY            S  E V+ E++RL E+Y    GE LSIT
Sbjct: 240 NGFRCPDPAVKAESGFLDLYTDKDTSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSIT 299

Query: 346 VTGHSLGAALALLVADEIS------VCSPEIPPVAVFSFGGPRVGNKAFANRVKSRNVKV 405
           VTGHSLG ALA+L A +++          ++ PV  F++GGPRVGN  F  R++   VKV
Sbjct: 300 VTGHSLGGALAVLSAYDVAEMGVNRTRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKV 359

Query: 406 LRMVNSQDVITQVPAV-------------------RYSHVGTELRVETKMSPYLKPNADV 437
           LR+VN  DV+ + P +                    YSHVG  L ++ + SP+LKP  D+
Sbjct: 360 LRVVNEHDVVAKSPGLFLNERAPQALMKLAGGLPWCYSHVGEMLPLDHQKSPFLKPTVDL 419

[Close]

BLAST of MS012289.1 vs. ExPASy TrEMBL
Match: A0A5D3E612 (Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G003630 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.4e-244
Identity = 422/502 (84.06%), Postives = 453/502 (90.24%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS F LRRSSFKY+ SPLNP+A KISSF+ AA +  V RRHL+NLDKLL K
Sbjct: 5   INSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLNK 64

Query: 63  TEPPAKTEPQPVHKDPNKG---LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRL 122
           T+PP K +PQPVHKDPNKG   +PIKGKGLLEGLNLSR+WPE+KA EEMSPRNLNRLQRL
Sbjct: 65  TDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQRL 124

Query: 123 LSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 182
           LSKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 
Sbjct: 125 LSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPT 184

Query: 183 TSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVC 242
           TSP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVC
Sbjct: 185 TSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVC 244

Query: 243 DDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLY 302
           DDRREIARMGRRDIVI+LRGTATCLEWAENVRA LIDI AD      GDPKVECGFLSLY
Sbjct: 245 DDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVDTKDCGDPKVECGFLSLY 304

Query: 303 RTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPP 362
           +T GAHV+SL+ESVVEEIRRLTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PP
Sbjct: 305 KTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAEVPP 364

Query: 363 VAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKM 422
           VAVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQDVITQVP   + YSHVGTELRVETKM
Sbjct: 365 VAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVETKM 424

Query: 423 SPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKV 482
           SP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KV
Sbjct: 425 SPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKV 484

Query: 483 KDLGRLNPEFHAAGPGCLPSPS 493
           KDLG  NPE      GCLPSPS
Sbjct: 485 KDLG-FNPELQTV--GCLPSPS 503

[Close]

BLAST of MS012289.1 vs. ExPASy TrEMBL
Match: A0A1S3CPU9 (phospholipase A1-Ibeta2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503457 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.4e-244
Identity = 422/502 (84.06%), Postives = 453/502 (90.24%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS F LRRSSFKY+ SPLNP+A KISSF+ AA +  V RRHL+NLDKLL K
Sbjct: 5   INSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLNK 64

Query: 63  TEPPAKTEPQPVHKDPNKG---LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRL 122
           T+PP K +PQPVHKDPNKG   +PIKGKGLLEGLNLSR+WPE+KA EEMSPRNLNRLQRL
Sbjct: 65  TDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQRL 124

Query: 123 LSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 182
           LSKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 
Sbjct: 125 LSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPT 184

Query: 183 TSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVC 242
           TSP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVC
Sbjct: 185 TSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVC 244

Query: 243 DDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLY 302
           DDRREIARMGRRDIVI+LRGTATCLEWAENVRA LIDI AD      GDPKVECGFLSLY
Sbjct: 245 DDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVDTKDCGDPKVECGFLSLY 304

Query: 303 RTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPP 362
           +T GAHV+SL+ESVVEEIRRLTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PP
Sbjct: 305 KTAGAHVRSLSESVVEEIRRLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAEVPP 364

Query: 363 VAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKM 422
           VAVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQDVITQVP   + YSHVGTELRVETKM
Sbjct: 365 VAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVETKM 424

Query: 423 SPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKV 482
           SP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KV
Sbjct: 425 SPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKV 484

Query: 483 KDLGRLNPEFHAAGPGCLPSPS 493
           KDLG  NPE      GCLPSPS
Sbjct: 485 KDLG-FNPELQTV--GCLPSPS 503

[Close]

BLAST of MS012289.1 vs. ExPASy TrEMBL
Match: A0A5A7T4U1 (Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G00160 PE=4 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 1.6e-243
Identity = 421/502 (83.86%), Postives = 453/502 (90.24%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS F LRRSSFKY+ SPLNP+A KISSF+ AA +  V RRHL+NLDKLL K
Sbjct: 5   INSTIPPQNLSHFHLRRSSFKYQISPLNPSAPKISSFRSAAVAVQVTRRHLANLDKLLNK 64

Query: 63  TEPPAKTEPQPVHKDPNKG---LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRL 122
           T+PP K +PQPVHKDPNKG   +PIKGKGLLEGLNLSR+WPE+KA EEMSPRNLNRLQRL
Sbjct: 65  TDPPLKVDPQPVHKDPNKGSLHMPIKGKGLLEGLNLSRLWPEVKAPEEMSPRNLNRLQRL 124

Query: 123 LSKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPA 182
           LSKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP 
Sbjct: 125 LSKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPT 184

Query: 183 TSPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVC 242
           TSP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVC
Sbjct: 185 TSPNEPPLPRHVTLPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVC 244

Query: 243 DDRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLY 302
           DDRREIARMGRRDIVI+LRGTATCLEWAENVRA LIDI AD      GDPKVECGFLSLY
Sbjct: 245 DDRREIARMGRRDIVIALRGTATCLEWAENVRAQLIDIPADVDTKDCGDPKVECGFLSLY 304

Query: 303 RTGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPP 362
           +T GAHV+SL+ESVVEEIR+LTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PP
Sbjct: 305 KTAGAHVRSLSESVVEEIRQLTEMYKGETLSITVTGHSLGAALAILVADEISVCSAEVPP 364

Query: 363 VAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKM 422
           VAVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQDVITQVP   + YSHVGTELRVETKM
Sbjct: 365 VAVFSFGGPRVGNKMFADRIKSRNVKVLRIVNSQDVITQVPPNPMTYSHVGTELRVETKM 424

Query: 423 SPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKV 482
           SP+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KV
Sbjct: 425 SPFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKV 484

Query: 483 KDLGRLNPEFHAAGPGCLPSPS 493
           KDLG  NPE      GCLPSPS
Sbjct: 485 KDLG-FNPELQTV--GCLPSPS 503

[Close]

BLAST of MS012289.1 vs. ExPASy TrEMBL
Match: A0A0A0LEL7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914060 PE=4 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.6e-240
Identity = 415/501 (82.83%), Postives = 452/501 (90.22%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPTA-KISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIP QNLS+F LRRSSFKY+ SPLNP+A KI+SF+ AA +  V RRHL+NLDKLL K
Sbjct: 4   INSTIPPQNLSQFHLRRSSFKYQISPLNPSAPKITSFRSAAVAVHVTRRHLANLDKLLNK 63

Query: 63  TEPPAKTEPQPVHKDPNK--GLPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRLL 122
           T+PP K +PQPVHKDPNK   +PIKGKGLLE LNL+R+WPE+KAAE+MSPRNLNRLQRLL
Sbjct: 64  TDPPLKVDPQPVHKDPNKVSPMPIKGKGLLESLNLARLWPEVKAAEDMSPRNLNRLQRLL 123

Query: 123 SKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT 182
           SKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQAAYHAFHSNP T
Sbjct: 124 SKTVEYSPRNALGQRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPTT 183

Query: 183 SPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCD 242
           SP+E PLPRHV+LPDRSYKVTKSLYATSSVGLP W+D VAPDLGWMTQRSSW+GYVAVCD
Sbjct: 184 SPNEPPLPRHVALPDRSYKVTKSLYATSSVGLPGWIDEVAPDLGWMTQRSSWVGYVAVCD 243

Query: 243 DRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD------GDPKVECGFLSLYR 302
           DRREIARMGRRDIVI+LRGTATCLEWAENVRA L ++ AD      GDPKVECGFLSLY+
Sbjct: 244 DRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNVPADVDTKDGGDPKVECGFLSLYK 303

Query: 303 TGGAHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPV 362
           T GAHVKSL+ESVVEEIRRLTE Y+GETLSITVTGHSLGAALA+LVADEISVCS E+PPV
Sbjct: 304 TAGAHVKSLSESVVEEIRRLTELYKGETLSITVTGHSLGAALAILVADEISVCSAEVPPV 363

Query: 363 AVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVP--AVRYSHVGTELRVETKMS 422
           AVFSFGGPRVGNK FA+R+KSRNVKVLR+VNSQD+ITQVP   + YSHVGTELRVETKMS
Sbjct: 364 AVFSFGGPRVGNKIFADRIKSRNVKVLRIVNSQDLITQVPPNPMTYSHVGTELRVETKMS 423

Query: 423 PYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKVK 482
           P+LKPNAD+ACCHDLEAYLHLVDGF+SSKCPFRPNAKRSL RLVQDQ+GN+KKLYM KVK
Sbjct: 424 PFLKPNADIACCHDLEAYLHLVDGFMSSKCPFRPNAKRSLVRLVQDQRGNMKKLYMRKVK 483

Query: 483 DLGRLNPEFHAAGPGCLPSPS 493
           DLG  NPE      GCLPSPS
Sbjct: 484 DLG-FNPELQTV--GCLPSPS 501

[Close]

BLAST of MS012289.1 vs. ExPASy TrEMBL
Match: A0A6J1EV72 (phospholipase A1-Ibeta2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111438059 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.6e-240
Identity = 417/496 (84.07%), Postives = 449/496 (90.52%), Query Frame = 0


Query: 3   IGSTIPAQNLSRFQLRRSSFKYEKSPLNPT-AKISSFKPAAGSCDVNRRHLSNLDKLLQK 62
           I STIPAQNLSRFQ+RRSSFKY+ SPLNP+ AKIS F+PA  + D  RRHL+NLDKLL+K
Sbjct: 4   INSTIPAQNLSRFQVRRSSFKYQMSPLNPSAAKISYFRPATAAVDGTRRHLANLDKLLKK 63

Query: 63  TEPPAKTEPQPVHKDPNKG--LPIKGKGLLEGLNLSRIWPEMKAAEEMSPRNLNRLQRLL 122
           T+ PAK EP+PV KDPNKG  +PIKGKGLLEGLNL+RIWPEM+AAE+MSPRNLNRLQRLL
Sbjct: 64  TDTPAKVEPEPVFKDPNKGSVMPIKGKGLLEGLNLARIWPEMRAAEDMSPRNLNRLQRLL 123

Query: 123 SKTVEYSPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPAT 182
           SKTVEYSPRNALG RWREYHGCNDW+GLLDPLDENLRREVVRYGEFVQ+AYH+FHSNPA 
Sbjct: 124 SKTVEYSPRNALGRRWREYHGCNDWSGLLDPLDENLRREVVRYGEFVQSAYHSFHSNPAM 183

Query: 183 SPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCD 242
           S +EAPLPRHV+LPD+SYKVTKSLYATSSVGLP WVD VAPDLGWMTQRSSW+GYVAVCD
Sbjct: 184 SLNEAPLPRHVALPDKSYKVTKSLYATSSVGLPGWVDEVAPDLGWMTQRSSWMGYVAVCD 243

Query: 243 DRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDAD---GDPKVECGFLSLYRTGG 302
           DRREIARMGRRDIVI+LRGTATCLEWAENVRA L +I  D    DPKVECGFLSLY+T G
Sbjct: 244 DRREIARMGRRDIVIALRGTATCLEWAENVRAQLTNIPTDTNSSDPKVECGFLSLYKTAG 303

Query: 303 AHVKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPVAVF 362
           AHVKSL+ESVVEEIRRL E Y+GETLSI+VTGHSLGAALALLVADEISVCSP +PPVAVF
Sbjct: 304 AHVKSLSESVVEEIRRLMEIYKGETLSISVTGHSLGAALALLVADEISVCSPTVPPVAVF 363

Query: 363 SFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAVRYSHVGTELRVETKMSPYLKP 422
           SFGGPRVGN AFANR+ SRNVKVLR+VNSQD+ITQVP V YSHVGTELRVETKMSPYLKP
Sbjct: 364 SFGGPRVGNTAFANRITSRNVKVLRIVNSQDLITQVPPVTYSHVGTELRVETKMSPYLKP 423

Query: 423 NADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRSLARLVQDQKGNLKKLYMSKVKDLGRL 482
           NADVACCHDLEAYLHLVDGFLSSKCPFR NAKRSL RLV DQ+GN+KKLYM KVKDL  L
Sbjct: 424 NADVACCHDLEAYLHLVDGFLSSKCPFRANAKRSLVRLVHDQRGNMKKLYMRKVKDLS-L 483

Query: 483 NPEFHAAGPGCLPSPS 493
           NPE      GCLPSPS
Sbjct: 484 NPELQML--GCLPSPS 496

[Close]

BLAST of MS012289.1 vs. TAIR 10
Match: AT4G16820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 549.3 bits (1414), Expect = 3.2e-156
Identity = 302/524 (57.63%), Postives = 355/524 (67.75%), Query Frame = 0


Query: 8   PAQNLSRFQLRRSSFKYEKSPLNPTAKISSFKPAAGSCDVNRRHLSNLDKLLQKTEPPAK 67
           P  ++   + RR +     S L PT K  S  PA      N+ HL NL+ +L+ +     
Sbjct: 6   PNADIFHAKRRRFTCNTHTSTLIPT-KPLSVSPAR---KTNKEHLRNLENVLRTSSNSID 65

Query: 68  TEPQPVHKDPNKGLPIKGKGLLEGLNLSRIWPEMKAA-EEMSPRNLNRLQRLLSK-TVEY 127
                  +            LL GLNL+RIWP+MKAA +EMSP+NL RLQRLLSK + E 
Sbjct: 66  HIENVTSRQEKTTKNTSTSSLLGGLNLARIWPQMKAAVDEMSPKNLKRLQRLLSKSSEER 125

Query: 128 SPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSPDEAP 187
           SP++ LG +WRE HG N+WAGLLDPLDENLRRE+VRYGEFVQAAYHAFHS+P  S     
Sbjct: 126 SPKSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGS----- 185

Query: 188 LPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIA 247
            PRHV+LPD S+KVTKSLYATSSV LP W+D VAPDL WMT+++SW+GYVAVCDD REI 
Sbjct: 186 -PRHVALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDDPREIR 245

Query: 248 RMGRRDIVISLRGTATCLEWAENVRAHLI-------DIDADGDPKVECGFLSLYRTGGAH 307
           RMGRR+IVI+LRGTAT LEW+EN R +L+       D      PKVECGF SLY TG  H
Sbjct: 246 RMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYTTGDQH 305

Query: 308 VKSLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPVAVFSF 367
             SLAES+V EI RL E Y GE LSI+VTGHSLGAA+ALL AD+I+   P  PPVAVFSF
Sbjct: 306 APSLAESLVGEISRLVELYAGEELSISVTGHSLGAAIALLAADDIAERVPHAPPVAVFSF 365

Query: 368 GGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV----------------------- 427
           GGPRVGN+ FA+R+ S+ VKVLR+VNSQDV+T+VP +                       
Sbjct: 366 GGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNGRSPGGIMEM 425

Query: 428 -------RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAK 487
                   YSHVG ELRV+ KMSPYLKPNADVACCHDLEAYLHLVDGFL+S CPFR NAK
Sbjct: 426 VERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLASNCPFRANAK 485

Query: 488 RSLARLVQDQKGNLKKLYMSKVKDLGRLNPEFHAAGPGCLPSPS 493
           RSL +L+ +Q+ N+K LY  K     RLN  FH  G   LPSPS
Sbjct: 486 RSLRKLLDEQRSNVKVLYTGKSL---RLNRTFHDNG-DVLPSPS 515

[Close]

BLAST of MS012289.1 vs. TAIR 10
Match: AT2G44810.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.0e-86
Identity = 172/363 (47.38%), Postives = 224/363 (61.71%), Query Frame = 0


Query: 137 EYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSP------DEAPLPRHV 196
           EY G  +W GLLDPLD+NLRRE++RYG+FV++AY AF  +P++          + L    
Sbjct: 2   EYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERS 61

Query: 197 SLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIARMGRR 256
            LP+  Y++TK+L ATS + LP W++  AP   WM  +SSWIGYVAVC D+ EI+R+GRR
Sbjct: 62  GLPNSGYRLTKNLRATSGINLPRWIEK-AP--SWMATQSSWIGYVAVCQDKEEISRLGRR 121

Query: 257 DIVISLRGTATCLEWAENVRAHLIDI----------DADGDPKVECGFLSLYRTGGAHVK 316
           D+VIS RGTATCLEW EN+RA L  +           ++  P VE GFLSLY +G   V 
Sbjct: 122 DVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLYTSG---VH 181

Query: 317 SLAESVVEEIRRLTEQYRGETLSITVTGHSLGAALALLVADEISVCSPEIPPVAVFSFGG 376
           SL + V EEI RL + Y  E LS+T+TGHSLGAA+A L A +I       P V V SFGG
Sbjct: 182 SLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYDIKTTFKRAPMVTVISFGG 241

Query: 377 PRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV------------------------- 436
           PRVGN+ F   ++ +  KVLR+VNS DVIT+VP V                         
Sbjct: 242 PRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRRV 301

Query: 437 -----RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKRS 454
                 Y+ +G ELR+ ++ SP+L  + +VA CH+L+ YLHLVDGF+SS CPFR  A+R 
Sbjct: 302 EETPWVYAEIGKELRLSSRDSPHLS-SINVATCHELKTYLHLVDGFVSSTCPFRETARRV 357

[Close]

BLAST of MS012289.1 vs. TAIR 10
Match: AT1G30370.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 5.6e-68
Identity = 146/363 (40.22%), Postives = 211/363 (58.13%), Query Frame = 0


Query: 126 SPRNALGGRWREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNP------AT 185
           SPR  +   WRE HG N+W  LLDPL   LRREV +YGEFV++ Y +   +P      ++
Sbjct: 109 SPREKISKMWRELHGSNNWENLLDPLHPWLRREVTKYGEFVESVYDSLDFDPLSEFCGSS 168

Query: 186 SPDEAPLPRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCD 245
             +   L   + L    YKVTK +YA S V +P W    A    W ++ S+W+G+VAV  
Sbjct: 169 RYNRNKLFEELGLTRHGYKVTKYIYAMSRVDVPQWFLSSALGETW-SKDSNWMGFVAVSG 228

Query: 246 DRREIARMGRRDIVISLRGTATCLEWAENVRAHLIDIDADGD-----PKVECGFLSLYRT 305
           D RE  R+GRRDIV++ RGT T  EW  ++R  +   D +G       KV+ GFLS+Y +
Sbjct: 229 D-RESLRIGRRDIVVAWRGTVTPTEWFMDLRTSMEPFDCEGKHGKTVVKVQSGFLSIYNS 288

Query: 306 GGA----HVKSLAESVVEEIRRLTEQY--RGETLSITVTGHSLGAALALLVADEISVCSP 365
                  + +S +E  ++E++RL   +  RGE +S+T+TGHSLG ALAL+ A E +   P
Sbjct: 289 KSELTRYNKESASEQTMDEVKRLVNFFKDRGEEVSLTITGHSLGGALALMNAYEAARDVP 348

Query: 366 EIP-PVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV------------ 425
            +   ++V SFG PRVGN AF  ++ S  VKVLR+VN QD++ ++P +            
Sbjct: 349 ALSGNISVISFGAPRVGNLAFKEKLNSLGVKVLRVVNKQDIVPKLPGIVFNKVLNKLNPI 408

Query: 426 ------RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLSSKCPFRPNAKR 453
                  Y HVGT+L+++   SPY+K ++D+   H+LE YLH++DGF   K  FR NA+R
Sbjct: 409 TSRLNWVYRHVGTQLKLDVFSSPYVKRDSDLGRAHNLEVYLHVLDGFHRKKSGFRVNARR 468

[Close]

BLAST of MS012289.1 vs. TAIR 10
Match: AT1G51440.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 3.1e-66
Identity = 144/347 (41.50%), Postives = 196/347 (56.48%), Query Frame = 0


Query: 135 WREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATS--------PDEAPL 194
           WRE  GCN+W G LDP++ +LRRE++RYGEF QA Y +F  +P +         P +  L
Sbjct: 91  WREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKYHPSDFFL 150

Query: 195 PRHVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIAR 254
              + L  + Y +T+ LYATS++ LP++         W +Q ++W+G+VAV  D  E++R
Sbjct: 151 NLDLHL-HKGYTITRYLYATSNINLPNFFQKSKLSSIW-SQHANWMGFVAVATDEEEVSR 210

Query: 255 MGRRDIVISLRGTATCLEWAENVRAHLIDIDADGDP--KVECGFLSLYRTGGAHVK---- 314
           +GRRDIVI+ RGT T LEW  +++  L   +   DP  K+E GF  LY       K    
Sbjct: 211 LGRRDIVIAWRGTVTYLEWIYDLKDILCSANFGDDPSIKIELGFHDLYTKKEDSCKFSSF 270

Query: 315 SLAESVVEEIRRLTEQY----RGETLSITVTGHSLGAALALLVADEISVCS-PEIP---- 374
           S  E V+ E++RL E Y     G   SITVTGHSLGA+LAL+ A +I+  +   +P    
Sbjct: 271 SAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAELNLNHVPENNY 330

Query: 375 --PVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV-------------- 434
             P+ VFSF GPRVGN  F  R     VKVLR+VN  D +  VP +              
Sbjct: 331 KIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNEKFQFQKYVEE 390

Query: 435 ------RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGF 437
                  Y+HVG EL ++ K SP+LKP  D+ C H+LEA LHLVDG+
Sbjct: 391 KTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLEALLHLVDGY 435

[Close]

BLAST of MS012289.1 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-61
Identity = 138/348 (39.66%), Postives = 198/348 (56.90%), Query Frame = 0


Query: 135 WREYHGCNDWAGLLDPLDENLRREVVRYGEFVQAAYHAFHSNPATSP------DEAPLPR 194
           WR+  G +DWAGL+DP+D  LR E++RYGE  QA Y AF  +PA+               
Sbjct: 105 WRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTRLEFFD 164

Query: 195 HVSLPDRSYKVTKSLYATSSVGLPDWVDHVAPDLGWMTQRSSWIGYVAVCDDRREIARMG 254
            + + D  Y+V + LYATS++ LP++         W ++ ++W+GYVAV DD     R+G
Sbjct: 165 SLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVW-SKNANWMGYVAVSDDETSRNRLG 224

Query: 255 RRDIVISLRGTATCLEWAENVRAHLIDIDAD----GDP--KVECGFLSLYRTGGAHVK-- 314
           RRDI I+ RGT T LEW  +++ +L  +  +     DP  KVE GFL LY       K  
Sbjct: 225 RRDIAIAWRGTVTKLEWIADLKDYLKPVTENKIRCPDPAVKVESGFLDLYTDKDTTCKFA 284

Query: 315 --SLAESVVEEIRRLTEQYRGE---TLSITVTGHSLGAALALLVADEISVC------SPE 374
             S  E ++ E++RL E++  +    LSITVTGHSLG ALA+L A +I+          +
Sbjct: 285 RFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNRSKKGK 344

Query: 375 IPPVAVFSFGGPRVGNKAFANRVKSRNVKVLRMVNSQDVITQVPAV-------------- 434
           + PV V ++GGPRVGN  F  R++   VKV+R+VN  DV+ + P +              
Sbjct: 345 VIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESRPHALMKIA 404

Query: 435 -----RYSHVGTELRVETKMSPYLKPNADVACCHDLEAYLHLVDGFLS 439
                 YSHVG EL ++ + SP+LKP+ DV+  H+LEA LHL+DG++S
Sbjct: 405 EGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLDGYVS 451

The following BLAST results are available for this feature:

BLAST of MS012289.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887009.1	8.7e-249	85.63	phospholipase A1-Ibeta2, chloroplastic [Benincasa hispida]	[more]
XP_008465868.2	1.1e-243	84.06	PREDICTED: phospholipase A1-Ibeta2, chloroplastic [Cucumis melo] >TYK31086.1 pho...	[more]
KAA0038492.1	3.2e-243	83.86	phospholipase A1-Ibeta2 [Cucumis melo var. makuwa]	[more]
XP_004148351.1	3.3e-240	82.83	phospholipase A1-Ibeta2, chloroplastic [Cucumis sativus] >KGN60475.1 hypothetica...	[more]
XP_022931784.1	7.4e-240	84.07	phospholipase A1-Ibeta2, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of MS012289.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23522	4.5e-155	57.63	Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16...	[more]
Q948R1	2.8e-88	46.98	Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 P...	[more]
Q9C8J6	4.3e-65	41.50	Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2...	[more]
Q3EBR6	7.1e-60	39.60	Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3...	[more]
Q941F1	9.3e-60	37.77	Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4...	[more]

BLAST of MS012289.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E612	5.4e-244	84.06	Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CPU9	5.4e-244	84.06	phospholipase A1-Ibeta2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503457 P...	[more]
A0A5A7T4U1	1.6e-243	83.86	Phospholipase A1-Ibeta2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A0A0LEL7	1.6e-240	82.83	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G914060 PE...	[more]
A0A6J1EV72	3.6e-240	84.07	phospholipase A1-Ibeta2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of MS012289.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16820.1	3.2e-156	57.63	alpha/beta-Hydrolases superfamily protein	[more]
AT2G44810.1	2.0e-86	47.38	alpha/beta-Hydrolases superfamily protein	[more]
AT1G30370.1	5.6e-68	40.22	alpha/beta-Hydrolases superfamily protein	[more]
AT1G51440.1	3.1e-66	41.50	alpha/beta-Hydrolases superfamily protein	[more]
AT2G30550.1	1.3e-61	39.66	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 134..475 e-value: 2.0E-102 score: 345.3
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 155..436
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 253..395 e-value: 2.1E-34 score: 118.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..85
None	No IPR available	PANTHER	PTHR31403	PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC	coord: 396..478
None	No IPR available	PANTHER	PTHR31403:SF37	GLYCEROLIPASE A1	coord: 396..478
None	No IPR available	PANTHER	PTHR31403	PHOSPHOLIPASE A1-IBETA2, CHLOROPLASTIC	coord: 1..395
None	No IPR available	PANTHER	PTHR31403:SF37	GLYCEROLIPASE A1	coord: 1..395
None	No IPR available	CDD	cd00519	Lipase_3	coord: 186..434 e-value: 5.50777E-34 score: 126.052

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS012289	MS012289	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS012289.1-cds	MS012289.1-cds-scaffold797:1235663..1237138	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS012289.1	MS012289.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process