MS003341.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS003341.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Methyl esterase 17
Location	scaffold234: 2227712 .. 2231130 (-)
Sequence length	831
RNA-Seq Expression	MS003341.1
Synteny	MS003341.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAGGAAGATAACCTGGCGCCAAGTGCCGTCTCTGTAGCAGTGCAGAAGCCGCAGCCGCAGACGCCGCACTTCGTGCTCGTTCACGGCATAAGTGGAGGAGGATGGTGCTGGTACAGAATCAGGTGTTTGATGGAGAATTCCGGCTTCAAGGTTACCTGTATTGATCTCAAAGGCGCCGGAATCGATCGCTCCGATCCTAATTCCGTCATCAAATTCGACGATTACAATAAGCCTCTGCTCGACTTCATCTCCACTCTGCCGGAGAACGAACAGGTATTTAATTTGTCGATGTATTTAATTTATTCGTCGGAAGTCTCTGTTTCTTCTATCGATTATACGATCGGGCTGAAAGTTAATTTTGAGAAATTTTTGTTGAAACTTTTACAGATCATACTGGTTGGACATAGCGCCGGAGGATTAAGTGTGACTCAAGCAACTCTCAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTACGTAGCGGCGACGATGCTAAAATTAGGCTACCAGACCGATCAAGATATCAAAGACGTAAACAATCCTCTTCTTCTCTCTGCTTCTATTCATGAAATTTCATGTGTGTATGTTTAGAAATGCTAATTGAAGTAATCGTAGCGTAGTAGTGCAGCGTTTTAGTATGAATTTTCCGGCCGGACGGCGATGTTATTGTTAAACTCGTCGCCGGCCGGTGGCGGTTTAGAAGATTCAGGGCCAGGGGAAGTGAGAACTGAGAAGATGATGGCCAATGTGTGAATAGTTGGCGGTTAGGAAATCGGAGGCGCTTGCTCTCATCGCAATCGCACGAGGCAAATAACATTTAGTCAATTAATTAGTCTCATTATTTTTGTCTTTAATTAAGAGAATGGATTAAAGACAGCCACCGGCCAGCTGGCTGCTAATTAACCATTTCTTATCCCCTATTACTGTAGTACTAATGCCCATCCTCTCTGACTCATAATAGTCTGAGAAGATTCCATTATGTTAAATAATTCACATAAGTTTCTGTATTCTTTTTGTGCAAAATTTAATTCAGATATACTTTATTTTTGTCGGTTACTTCATTTTTACAATTTACTGAAACTACCTTATTTAGAAGGTCTAACTTTTTTTTTCCTTTCTAGAATATTAAAATTCAAACAAAAATTAGAGATTTAATCCTATGAAAAAAATATTGTCATTGATTTGACTGGCTGAGTTTTACTTAGGTCATGAATTGGTTATAGAAAATTACAAGCAGAGATTTTTTTATAAAAAGAATTTTCAGATTATTATAATTCATATATGATCTTAATTTGATAAATTGTCATAAAAATTTAGAGTTTGCATACATCACGGATGTGATTTTTCTGATGAAATAGATTGGTACAGTGGTGCCGCAGCACCAGAAAAGGACGTGTCCCTTTCTGTGTAGTGAAAAAAAAAAAATTGTGCTGTAAATTTTCTGTGCCACAGTGCTATGAAATATTTTTTTTATATAACTTTTAATATCTTTTAAGTTTTAACCATGTTTTTATTTGGCCCCTAATTTTAAGATATCTGAATCTGTATAATTAAGAGAAAAAGTTTTATGTTATAAACTTATAACAGAATAATTACAAAATTGTAGGTTCTTGTAGTTGTTTGATCTATAGATTTGTAATTTATGTGGATAGTTTAGGGGTGATTTTTTTTAGTAAATCAAAGGTATGGTGAGTTTGAACTTCTAACTTCTTAGAATATTACTTTTAATTGGTTGAGTTTGAACTTCTAACTTCTTAGAATGTTACTTTTAATTGGTTGAATTATGCTCAAGTCAGTAGAACACGATTTTAGTAGTTTTGATTTATGTCGTTTATAAAATTAGTAAAAGTGTATAAGATAGGTTTAAGATATTTATATTCCAAGTGAATTTGACTTTCAAAAATAATTACTAAGAATAGAAATTTTTCTCTTTTGGAGAGAAGAATTATTTTTTATTTTTTATTTTTATAAAAAATAAATAGTTGTCAAATTTTAAAAAATGACACGGTAATTTGTGAGTCAAGTATTGGTTTAAAATAAAATAAAATCTAATAGTGAGAATAATATAGATGGTGTGTATGTTACTACAGTGCCTAGGTGGAATATTTTGCCTGGTATGTACGGAATAAAGTCAAAATTGGGTTAGGCCAGTTGGAGTTTTCAAGGCCGCAAATAGTGTACTCAATCGGAGCCCGACAGCTTCCGCGAGTACACAGTTCTCACGTGTGCAGTCCACACATCTTTTTCTTTGTAAAGTTCATTACTTCACCGATTAAAGGAACTAACAATGCAAACCCATTCATTATCAATGGATTGGGGAACAAAATTTTCTACTGCTCTAAATAAGTGATTCACTCTAGAATTTTCAAGGTTTATTTATTAATTCTTTTTATTTTCTTTATAGAAAAAAGTCGTTCTCCAGCTTCATTATATTCCCTGCTTAAGTTGGCCAGAGAGTGCGTTTAACATATGGTCTTTTGAAATGGTTTAGTAAAAAAGGATTGGAGGAGAAAACTCCGACCCCCAATTGTCAAAGAAAGAAAGATTGGAGGAGAGTATACTTTGAGTAGAAGCTGGTGTGATTTTGAGATAATTATTCTTGTCTATATATGATGGACCTTTCAATTTCCTAGTTAAGGAAGAACTTTGTTTGTACTCAAGAATAGAGAGATTTAGTAAATAAACGACTTTTAGACCTGGTCCCGTGTCTTGGACGGCAGGAATTCCTCAGGAAGTAGAATCTGTCTTATAAATGTTGTCTTTAACCTTTTTTTTCTTTTCTAATCTTTCATCATTTCAATAGTACTGATGATAATTTGTTTATTTATGTATGTTGGTCAAATGTGTGCAGGGAGTGCCAGATTTATCCGAGTTTGGGGATGTTTATGACCTAGAATTTGGACTAGGGTCCCAACAACCTCCAACCACAGCCATCATCAAAAGGGAATTCCAGCGCAAGATCTGCTATCACATGAGCCCCCACGAGGTACCCAAAACCAAAATATTTATTGATCAAGATTATCAAAATTAGATTGAATTTTTTTTTAAATCTCTACTTCTTAATATGACCTTTTTTAGTTTAAGACAAACTAGGAGTAGGGGAAACATGAACCGTGTTTGAGATGTATGGCGTTTTTCAGGATTCTACGCTGGGCGCCATGCTTCTAAGGCCAGGGCCAATCCTGGCATTAAAGACTGCTCAGTTTAGAGAGGAGGAAGAATGTGCCGATAAGGTGAAACGAGTATTTATAAGAACAATGTATGATCGTGTAATCAAAAGGGAGCAACAAGATGCCATGATAAAGCGATGGCCACCCGAGATCGTATACGACATGGATACCGATCACAGCCCCTTCTTCTCCAACCCGTCTCTGCTCTTTGGCTTGCTTCTAAAGGCTGCTGCGTCAACAGGATGT

mRNA sequence

ATGGGAGAGGAAGATAACCTGGCGCCAAGTGCCGTCTCTGTAGCAGTGCAGAAGCCGCAGCCGCAGACGCCGCACTTCGTGCTCGTTCACGGCATAAGTGGAGGAGGATGGTGCTGGTACAGAATCAGGTGTTTGATGGAGAATTCCGGCTTCAAGGTTACCTGTATTGATCTCAAAGGCGCCGGAATCGATCGCTCCGATCCTAATTCCGTCATCAAATTCGACGATTACAATAAGCCTCTGCTCGACTTCATCTCCACTCTGCCGGAGAACGAACAGATCATACTGGTTGGACATAGCGCCGGAGGATTAAGTGTGACTCAAGCAACTCTCAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTACGTAGCGGCGACGATGCTAAAATTAGGCTACCAGACCGATCAAGATATCAAAGACGGAGTGCCAGATTTATCCGAGTTTGGGGATGTTTATGACCTAGAATTTGGACTAGGGTCCCAACAACCTCCAACCACAGCCATCATCAAAAGGGAATTCCAGCGCAAGATCTGCTATCACATGAGCCCCCACGAGGATTCTACGCTGGGCGCCATGCTTCTAAGGCCAGGGCCAATCCTGGCATTAAAGACTGCTCAGTTTAGAGAGGAGGAAGAATGTGCCGATAAGGTGAAACGAGTATTTATAAGAACAATGTATGATCGTGTAATCAAAAGGGAGCAACAAGATGCCATGATAAAGCGATGGCCACCCGAGATCGTATACGACATGGATACCGATCACAGCCCCTTCTTCTCCAACCCGTCTCTGCTCTTTGGCTTGCTTCTAAAGGCTGCTGCGTCAACAGGATGT

Coding sequence (CDS)

ATGGGAGAGGAAGATAACCTGGCGCCAAGTGCCGTCTCTGTAGCAGTGCAGAAGCCGCAGCCGCAGACGCCGCACTTCGTGCTCGTTCACGGCATAAGTGGAGGAGGATGGTGCTGGTACAGAATCAGGTGTTTGATGGAGAATTCCGGCTTCAAGGTTACCTGTATTGATCTCAAAGGCGCCGGAATCGATCGCTCCGATCCTAATTCCGTCATCAAATTCGACGATTACAATAAGCCTCTGCTCGACTTCATCTCCACTCTGCCGGAGAACGAACAGATCATACTGGTTGGACATAGCGCCGGAGGATTAAGTGTGACTCAAGCAACTCTCAAGTTCGCGAAGAAAATCCGTTTGGCGGTGTACGTAGCGGCGACGATGCTAAAATTAGGCTACCAGACCGATCAAGATATCAAAGACGGAGTGCCAGATTTATCCGAGTTTGGGGATGTTTATGACCTAGAATTTGGACTAGGGTCCCAACAACCTCCAACCACAGCCATCATCAAAAGGGAATTCCAGCGCAAGATCTGCTATCACATGAGCCCCCACGAGGATTCTACGCTGGGCGCCATGCTTCTAAGGCCAGGGCCAATCCTGGCATTAAAGACTGCTCAGTTTAGAGAGGAGGAAGAATGTGCCGATAAGGTGAAACGAGTATTTATAAGAACAATGTATGATCGTGTAATCAAAAGGGAGCAACAAGATGCCATGATAAAGCGATGGCCACCCGAGATCGTATACGACATGGATACCGATCACAGCCCCTTCTTCTCCAACCCGTCTCTGCTCTTTGGCTTGCTTCTAAAGGCTGCTGCGTCAACAGGATGT

Protein sequence

MGEEDNLAPSAVSVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC

Homology

[Close]

BLAST of MS003341.1 vs. NCBI nr
Match: XP_022134269.1 (methylesterase 17 [Momordica charantia])

HSP 1 Score: 565.5 bits (1456), Expect = 2.6e-157
Identity = 275/277 (99.28%), Postives = 275/277 (99.28%), Query Frame = 0


Query: 1   MGEEDNLAPSAVSVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKG 60
           MGEEDNLAPSAVSVAVQKP PQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKG
Sbjct: 1   MGEEDNLAPSAVSVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKG 60

Query: 61  AGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLA 120
           AGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLA
Sbjct: 61  AGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLA 120

Query: 121 VYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYH 180
           VYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYH
Sbjct: 121 VYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYH 180

Query: 181 MSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIK 240
           MSPHEDSTLGAMLLRPGPILALKTAQFREEEE ADKVKRVFIRTMYDRVIKREQQDAMIK
Sbjct: 181 MSPHEDSTLGAMLLRPGPILALKTAQFREEEEFADKVKRVFIRTMYDRVIKREQQDAMIK 240

Query: 241 RWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC 278
           RWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC
Sbjct: 241 RWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC 277

[Close]

BLAST of MS003341.1 vs. NCBI nr
Match: XP_038890470.1 (methylesterase 17 [Benincasa hispida])

HSP 1 Score: 495.7 bits (1275), Expect = 2.5e-136
Identity = 241/279 (86.38%), Postives = 262/279 (93.91%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPS+V SV+VQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSSVDSVSVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGIDRSDPNSV+KFDDYNKPLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRL
Sbjct: 61  GAGIDRSDPNSVLKFDDYNKPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTD+D KDGVPDLSEFGDVYD+EFGLG QQPPTTA+IKREFQRKI Y
Sbjct: 121 AVYVAATMLRFGFQTDRDFKDGVPDLSEFGDVYDVEFGLGPQQPPTTAVIKREFQRKIIY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMI 240
           HMSPHEDSTL AMLLRPGPILALKTAQF EE++ ADKVKRV+IRTMYDRVIK EQQDAMI
Sbjct: 181 HMSPHEDSTLAAMLLRPGPILALKTAQFMEEQDGADKVKRVYIRTMYDRVIKPEQQDAMI 240

Query: 241 KRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAA-STGC 278
           K WPPEIVY+MDTDHSPFFS+PSLLFGLL+K+ + S GC
Sbjct: 241 KGWPPEIVYEMDTDHSPFFSDPSLLFGLLVKSCSLSMGC 275

[Close]

BLAST of MS003341.1 vs. NCBI nr
Match: XP_022993026.1 (methylesterase 17 [Cucurbita maxima])

HSP 1 Score: 479.2 bits (1232), Expect = 2.4e-131
Identity = 234/275 (85.09%), Postives = 254/275 (92.36%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIK+GVPDLSEFGDVYD+ FGLG QQPPTTA+IKRE QRKI Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMI 240
           HMSPHEDSTL +MLLRPGPILAL+TA+FREE    D VKRV+IRT YDRVIK EQQ+AMI
Sbjct: 181 HMSPHEDSTLASMLLRPGPILALRTARFREENGELDTVKRVYIRTTYDRVIKPEQQEAMI 240

Query: 241 KRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAAS 275
           + W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+
Sbjct: 241 REWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAA 271

[Close]

BLAST of MS003341.1 vs. NCBI nr
Match: XP_022938905.1 (methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 476.1 bits (1224), Expect = 2.1e-130
Identity = 231/275 (84.00%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIK+GVPDLSEFGD YD+ FG G QQPPTTA++KRE QRKI Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMI 240
           HMSPHEDSTL +MLLRPGPILAL+TA+FREE    D+VKRV+IRT YDRVIK EQQ+AMI
Sbjct: 181 HMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMI 240

Query: 241 KRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAAS 275
           + W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+
Sbjct: 241 REWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAA 271

[Close]

BLAST of MS003341.1 vs. NCBI nr
Match: KAG6578491.1 (Methylesterase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 476.1 bits (1224), Expect = 2.1e-130
Identity = 231/275 (84.00%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIK+GVPDLSEFGD YD+ FG G QQPPTTA++KRE QRKI Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMI 240
           HMSPHEDSTL +MLLRPGPILAL+TA+FREE    D+VKRV+IRT YDRVIK EQQ+AMI
Sbjct: 181 HMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMI 240

Query: 241 KRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAAS 275
           + W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+
Sbjct: 241 REWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAA 271

[Close]

BLAST of MS003341.1 vs. ExPASy Swiss-Prot
Match: Q9SG92 (Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 4.8e-98
Identity = 165/262 (62.98%), Postives = 212/262 (80.92%), Query Frame = 0


Query: 18  KPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDY 77
           KP  + PHFVL+HG+S G WCWY+I+CLME SGF VTCIDLK +GID S  +S+  FD Y
Sbjct: 13  KPSRKPPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQY 72

Query: 78  NKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQD 137
           N+PL+DF+S+ PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+MLK G QTD+D
Sbjct: 73  NQPLIDFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDED 132

Query: 138 IKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPG 197
           +KDGVPDLSE GDVY+L FGLG + PPT+AIIK E++RK+ YHMSP ++ +L A+++RP 
Sbjct: 133 MKDGVPDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQECSLAALMMRPA 192

Query: 198 PILALKTAQFREEEE---CADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDMDTDH 257
           PILAL TA+  EEE+     ++V RV+I+T+ DRV+K EQQDAMI+RWPP  VY++++DH
Sbjct: 193 PILALTTAKLEEEEKEKGQEEQVPRVYIKTLLDRVMKPEQQDAMIRRWPPSQVYELESDH 252

Query: 258 SPFFSNPSLLFGLLLKAAASTG 277
           SPFFSNP +LFGLL+KAA S G
Sbjct: 253 SPFFSNPFVLFGLLIKAAVSVG 274

[Close]

BLAST of MS003341.1 vs. ExPASy Swiss-Prot
Match: Q2V0W1 (Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.2e-56
Identity = 109/250 (43.60%), Postives = 158/250 (63.20%), Query Frame = 0


Query: 25  HFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDF 84
           HFV VHG S G WCWY++  L+  +GFK T +DL GAGI+ +D N+V  FD YN+PL   
Sbjct: 13  HFVFVHGASHGAWCWYKLTTLLVAAGFKATSVDLTGAGINLTDSNTVFDFDHYNRPLFSL 72

Query: 85  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPD 144
           +S LP + +I+LVGHS GG SVT+A  KF  KI + VY+AA M++ G  +     D +  
Sbjct: 73  LSDLPSHHKIVLVGHSIGGGSVTEALCKFTDKISMVVYLAADMVQPG--STSSTHDSIMT 132

Query: 145 LSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPILALKT 204
           + E  D+++  +G G+ +PPT  ++K EF+R   Y  SP ED +L + LLRP P+ AL  
Sbjct: 133 VGE-EDIWEYIYGEGADKPPTGVLMKEEFRRHYYYSQSPLEDVSLASKLLRPAPVRALGG 192

Query: 205 AQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DTDHSPFFSNPSL 264
           A        A+KV RV+I+T  D +    +QD ++++WPP  +Y + ++DHS FFS P+ 
Sbjct: 193 ADKLSPNPEAEKVPRVYIKTAKDNLFDPLRQDRLVEKWPPSQLYILEESDHSAFFSVPTT 252

Query: 265 LFGLLLKAAA 274
           LF  LL+A +
Sbjct: 253 LFAYLLRAVS 259

[Close]

BLAST of MS003341.1 vs. ExPASy Swiss-Prot
Match: Q9LVL9 (Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.9e-54
Identity = 110/256 (42.97%), Postives = 155/256 (60.55%), Query Frame = 0


Query: 25  HFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDF 84
           HFV VHG   GGWCWY++   + ++G K TCIDLKGAGI+ +DPN+V   DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 85  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPD 144
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M+K G    + +K+ +  
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 145 LS-----EFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPI 204
            S     E   ++D  FG G Q  PT+ ++K E+ R   Y+ SP ED TL   LLRP P+
Sbjct: 125 CSGLIEEETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYTLATTLLRPAPV 184

Query: 205 LALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DTDHSPFF 264
           +A             DK+ RV+++T  D + +   Q+ M+  WPP   + + D+DHS FF
Sbjct: 185 MAFIGIMDIPGAPETDKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTFLLPDSDHSAFF 244

Query: 265 SNPSLLFGLLLKAAAS 275
           S P  L+  LL+AA+S
Sbjct: 245 SQPQELYQFLLQAASS 260

[Close]

BLAST of MS003341.1 vs. ExPASy Swiss-Prot
Match: O23512 (Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.3e-54
Identity = 109/254 (42.91%), Postives = 152/254 (59.84%), Query Frame = 0


Query: 25  HFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDF 84
           HFV VHG S G WCWY++  L++ +GFK T +DL GAGI   D N V   D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 85  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPD 144
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G           P 
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPS-------PH 131

Query: 145 LSEF----GDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPIL 204
           LS       D+++  +G G+ +PPT  ++K EF R   Y  SP ED TL + LLRP P+ 
Sbjct: 132 LSNIHVGEEDIWEYTYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVTLSSKLLRPAPMR 191

Query: 205 ALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DTDHSPFFS 264
           A +          A+KV RV+I+T  D +    +QD +++ WPP  +Y + D+DHS FFS
Sbjct: 192 AFQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLYVLEDSDHSAFFS 251

Query: 265 NPSLLFGLLLKAAA 274
            P+ LF  LL+A +
Sbjct: 252 VPTTLFAYLLRAVS 258

[Close]

BLAST of MS003341.1 vs. ExPASy Swiss-Prot
Match: Q9FW03 (Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES11 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 9.3e-41
Identity = 98/260 (37.69%), Postives = 136/260 (52.31%), Query Frame = 0


Query: 22  QTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPL 81
           +T HFVLVHG S G WCWY+   L+E  GFKVT IDL G GI+  + N +     Y KPL
Sbjct: 135 ETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPL 194

Query: 82  LDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQ--DIK 141
            D +  LP  E++ILVGH  GG  ++ A   F  KI  AV++AA ML  G  T     +K
Sbjct: 195 TDILEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSLK 254

Query: 142 DGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPI 201
            G  DL     ++   +  G++ PPT   + +   + + ++ SP +D  L ++ +R  P 
Sbjct: 255 AGQNDLMRKAQIF--IYTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALASVSMRSIPF 314

Query: 202 ------LALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DT 261
                 L+L  A +         V+R +I T+ D  I    Q+ MI   PPE VY +   
Sbjct: 315 APVLEKLSLSDANY-------GSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGA 374

Query: 262 DHSPFFSNPSLLFGLLLKAA 273
           DH+PFFS P  L  LLL+ A
Sbjct: 375 DHAPFFSKPQALHKLLLEIA 385

[Close]

BLAST of MS003341.1 vs. ExPASy TrEMBL
Match: A0A6J1BYB8 (methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.3e-157
Identity = 275/277 (99.28%), Postives = 275/277 (99.28%), Query Frame = 0


Query: 1   MGEEDNLAPSAVSVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKG 60
           MGEEDNLAPSAVSVAVQKP PQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKG
Sbjct: 1   MGEEDNLAPSAVSVAVQKPLPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKG 60

Query: 61  AGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLA 120
           AGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLA
Sbjct: 61  AGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLA 120

Query: 121 VYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYH 180
           VYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYH
Sbjct: 121 VYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYH 180

Query: 181 MSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIK 240
           MSPHEDSTLGAMLLRPGPILALKTAQFREEEE ADKVKRVFIRTMYDRVIKREQQDAMIK
Sbjct: 181 MSPHEDSTLGAMLLRPGPILALKTAQFREEEEFADKVKRVFIRTMYDRVIKREQQDAMIK 240

Query: 241 RWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC 278
           RWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC
Sbjct: 241 RWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAASTGC 277

[Close]

BLAST of MS003341.1 vs. ExPASy TrEMBL
Match: A0A6J1JV68 (methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.2e-131
Identity = 234/275 (85.09%), Postives = 254/275 (92.36%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIK+GVPDLSEFGDVYD+ FGLG QQPPTTA+IKRE QRKI Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKNGVPDLSEFGDVYDVGFGLGPQQPPTTAVIKRELQRKIIY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMI 240
           HMSPHEDSTL +MLLRPGPILAL+TA+FREE    D VKRV+IRT YDRVIK EQQ+AMI
Sbjct: 181 HMSPHEDSTLASMLLRPGPILALRTARFREENGELDTVKRVYIRTTYDRVIKPEQQEAMI 240

Query: 241 KRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAAS 275
           + W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+
Sbjct: 241 REWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAA 271

[Close]

BLAST of MS003341.1 vs. ExPASy TrEMBL
Match: A0A6J1FL47 (methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.0e-130
Identity = 231/275 (84.00%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPSAV SVAVQKP    PHFVLVHGISGGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSAVGSVAVQKP----PHFVLVHGISGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSV+KFDDYNKPLLDF+S+LPENEQIILVGHSAGGLSVTQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVVKFDDYNKPLLDFVSSLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIK+GVPDLSEFGD YD+ FG G QQPPTTA++KRE QRKI Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKNGVPDLSEFGDAYDVGFGQGPQQPPTTAVVKRELQRKIIY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMI 240
           HMSPHEDSTL +MLLRPGPILAL+TA+FREE    D+VKRV+IRT YDRVIK EQQ+AMI
Sbjct: 181 HMSPHEDSTLASMLLRPGPILALRTARFREENGELDRVKRVYIRTTYDRVIKPEQQEAMI 240

Query: 241 KRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAAS 275
           + W PEIVY+MDTDHSPFFSNPSLLFGLL+KAAA+
Sbjct: 241 REWLPEIVYEMDTDHSPFFSNPSLLFGLLVKAAAA 271

[Close]

BLAST of MS003341.1 vs. ExPASy TrEMBL
Match: A0A5A7TBF5 (Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001670 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.2e-130
Identity = 230/276 (83.33%), Postives = 254/276 (92.03%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPS+V SV++  P    PHFVLVHGI GGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSSVNSVSLHNP----PHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSVI FDDYN+PLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIKDGVPDLSE+GD+Y+LEFGLG QQPPTTA+IKREFQRK+ Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEE--ECADKVKRVFIRTMYDRVIKREQQDA 240
           HMSPHEDSTL AMLLRPGPI AL +AQF+EEE  E ADKVKRV+IRTMYDRVIK EQQ+A
Sbjct: 181 HMSPHEDSTLAAMLLRPGPIQALMSAQFKEEEDREEADKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAA 274
           MIK WPPEIVY+MDTDHSPFFSNPSLLFGLL+K+ +
Sbjct: 241 MIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCS 272

[Close]

BLAST of MS003341.1 vs. ExPASy TrEMBL
Match: A0A1S3CAT2 (methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.2e-130
Identity = 230/276 (83.33%), Postives = 254/276 (92.03%), Query Frame = 0


Query: 1   MGEEDNLAPSAV-SVAVQKPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLK 60
           MGE++NLAPS+V SV++  P    PHFVLVHGI GGGWCWY+IRCLMENSGFKVTCIDLK
Sbjct: 1   MGEKENLAPSSVNSVSLHNP----PHFVLVHGIGGGGWCWYKIRCLMENSGFKVTCIDLK 60

Query: 61  GAGIDRSDPNSVIKFDDYNKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRL 120
           GAGID SDPNSVI FDDYN+PLLDFIS+LPENEQIILVGHSAGGLS+TQATLKFAKKIRL
Sbjct: 61  GAGIDLSDPNSVINFDDYNQPLLDFISSLPENEQIILVGHSAGGLSITQATLKFAKKIRL 120

Query: 121 AVYVAATMLKLGYQTDQDIKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICY 180
           AVYVAATML+ G+QTDQDIKDGVPDLSE+GD+Y+LEFGLG QQPPTTA+IKREFQRK+ Y
Sbjct: 121 AVYVAATMLRFGFQTDQDIKDGVPDLSEYGDIYNLEFGLGPQQPPTTAVIKREFQRKVAY 180

Query: 181 HMSPHEDSTLGAMLLRPGPILALKTAQFREEE--ECADKVKRVFIRTMYDRVIKREQQDA 240
           HMSPHEDSTL AMLLRPGPI AL +AQF+EEE  E ADKVKRV+IRTMYDRVIK EQQ+A
Sbjct: 181 HMSPHEDSTLAAMLLRPGPIQALMSAQFKEEEDREEADKVKRVYIRTMYDRVIKPEQQEA 240

Query: 241 MIKRWPPEIVYDMDTDHSPFFSNPSLLFGLLLKAAA 274
           MIK WPPEIVY+MDTDHSPFFSNPSLLFGLL+K+ +
Sbjct: 241 MIKGWPPEIVYEMDTDHSPFFSNPSLLFGLLIKSCS 272

[Close]

BLAST of MS003341.1 vs. TAIR 10
Match: AT3G10870.1 (methyl esterase 17 )

HSP 1 Score: 359.0 bits (920), Expect = 3.4e-99
Identity = 165/262 (62.98%), Postives = 212/262 (80.92%), Query Frame = 0


Query: 18  KPQPQTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDY 77
           KP  + PHFVL+HG+S G WCWY+I+CLME SGF VTCIDLK +GID S  +S+  FD Y
Sbjct: 13  KPSRKPPHFVLIHGMSLGSWCWYKIKCLMEVSGFTVTCIDLKSSGIDSSSVDSLTTFDQY 72

Query: 78  NKPLLDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQD 137
           N+PL+DF+S+ PE EQ+ILVGHSAGGLS+T A  +F KKI LAV++ A+MLK G QTD+D
Sbjct: 73  NQPLIDFLSSFPEQEQVILVGHSAGGLSLTSAIQRFPKKICLAVFIGASMLKNGLQTDED 132

Query: 138 IKDGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPG 197
           +KDGVPDLSE GDVY+L FGLG + PPT+AIIK E++RK+ YHMSP ++ +L A+++RP 
Sbjct: 133 MKDGVPDLSEHGDVYELGFGLGPENPPTSAIIKPEYRRKLLYHMSPQQECSLAALMMRPA 192

Query: 198 PILALKTAQFREEEE---CADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDMDTDH 257
           PILAL TA+  EEE+     ++V RV+I+T+ DRV+K EQQDAMI+RWPP  VY++++DH
Sbjct: 193 PILALTTAKLEEEEKEKGQEEQVPRVYIKTLLDRVMKPEQQDAMIRRWPPSQVYELESDH 252

Query: 258 SPFFSNPSLLFGLLLKAAASTG 277
           SPFFSNP +LFGLL+KAA S G
Sbjct: 253 SPFFSNPFVLFGLLIKAAVSVG 274

[Close]

BLAST of MS003341.1 vs. TAIR 10
Match: AT5G58310.1 (methyl esterase 18 )

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-55
Identity = 110/256 (42.97%), Postives = 155/256 (60.55%), Query Frame = 0


Query: 25  HFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDF 84
           HFV VHG   GGWCWY++   + ++G K TCIDLKGAGI+ +DPN+V   DDY++PL  F
Sbjct: 5   HFVFVHGAGHGGWCWYKLANSLRDNGHKATCIDLKGAGINPTDPNTVSSLDDYDEPLYAF 64

Query: 85  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPD 144
           +S LP ++++ILV HS GG S+T A   F  K+ LAVYVAA M+K G    + +K+ +  
Sbjct: 65  LSQLPNDQKVILVSHSVGGGSMTAAMCLFPSKVSLAVYVAAAMVKPGTLIPERLKNVMKI 124

Query: 145 LS-----EFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPI 204
            S     E   ++D  FG G Q  PT+ ++K E+ R   Y+ SP ED TL   LLRP P+
Sbjct: 125 CSGLIEEETEKIWDFTFGNGPQNLPTSIMMKPEYVRDKFYNESPMEDYTLATTLLRPAPV 184

Query: 205 LALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DTDHSPFF 264
           +A             DK+ RV+++T  D + +   Q+ M+  WPP   + + D+DHS FF
Sbjct: 185 MAFIGIMDIPGAPETDKIPRVYVKTGKDHLFEPVLQEVMLALWPPAHTFLLPDSDHSAFF 244

Query: 265 SNPSLLFGLLLKAAAS 275
           S P  L+  LL+AA+S
Sbjct: 245 SQPQELYQFLLQAASS 260

[Close]

BLAST of MS003341.1 vs. TAIR 10
Match: AT4G16690.1 (methyl esterase 16 )

HSP 1 Score: 213.0 bits (541), Expect = 3.0e-55
Identity = 109/254 (42.91%), Postives = 152/254 (59.84%), Query Frame = 0


Query: 25  HFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDF 84
           HFV VHG S G WCWY++  L++ +GFK T +DL GAGI   D N V   D YN+PL   
Sbjct: 12  HFVFVHGASHGAWCWYKLTTLLDAAGFKSTSVDLTGAGISLIDSNIVFDSDQYNRPLFSL 71

Query: 85  ISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPD 144
           +S LP + ++ILVGHS GG SVT+A  KF  KI +A+Y+AA+M++ G           P 
Sbjct: 72  LSDLPPHHKVILVGHSIGGGSVTEALCKFTDKISMAIYLAASMVQPGSIPS-------PH 131

Query: 145 LSEF----GDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPIL 204
           LS       D+++  +G G+ +PPT  ++K EF R   Y  SP ED TL + LLRP P+ 
Sbjct: 132 LSNIHVGEEDIWEYTYGEGTDKPPTGVLMKPEFIRHYYYSQSPLEDVTLSSKLLRPAPMR 191

Query: 205 ALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DTDHSPFFS 264
           A +          A+KV RV+I+T  D +    +QD +++ WPP  +Y + D+DHS FFS
Sbjct: 192 AFQDLDKLPPNPEAEKVPRVYIKTAKDNLFDSVRQDLLVENWPPSQLYVLEDSDHSAFFS 251

Query: 265 NPSLLFGLLLKAAA 274
            P+ LF  LL+A +
Sbjct: 252 VPTTLFAYLLRAVS 258

[Close]

BLAST of MS003341.1 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 168.7 bits (426), Expect = 6.6e-42
Identity = 98/260 (37.69%), Postives = 136/260 (52.31%), Query Frame = 0


Query: 22  QTPHFVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPL 81
           +T HFVLVHG S G WCWY+   L+E  GFKVT IDL G GI+  + N +     Y KPL
Sbjct: 135 ETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPL 194

Query: 82  LDFISTLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQ--DIK 141
            D +  LP  E++ILVGH  GG  ++ A   F  KI  AV++AA ML  G  T     +K
Sbjct: 195 TDILEKLPIGEKVILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSLK 254

Query: 142 DGVPDLSEFGDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPI 201
            G  DL     ++   +  G++ PPT   + +   + + ++ SP +D  L ++ +R  P 
Sbjct: 255 AGQNDLMRKAQIF--IYTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALASVSMRSIPF 314

Query: 202 ------LALKTAQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DT 261
                 L+L  A +         V+R +I T+ D  I    Q+ MI   PPE VY +   
Sbjct: 315 APVLEKLSLSDANY-------GSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGA 374

Query: 262 DHSPFFSNPSLLFGLLLKAA 273
           DH+PFFS P  L  LLL+ A
Sbjct: 375 DHAPFFSKPQALHKLLLEIA 385

[Close]

BLAST of MS003341.1 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 166.0 bits (419), Expect = 4.3e-41
Identity = 85/249 (34.14%), Postives = 140/249 (56.22%), Query Frame = 0


Query: 26  FVLVHGISGGGWCWYRIRCLMENSGFKVTCIDLKGAGIDRSDPNSVIKFDDYNKPLLDFI 85
           FVLVHG   G WCWY++   +E SG     +DL G G + +D N+V   ++Y+KPL+D +
Sbjct: 98  FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLL 157

Query: 86  STLPENEQIILVGHSAGGLSVTQATLKFAKKIRLAVYVAATMLKLGYQTDQDIKDGVPDL 145
             LPE E++ILVGHS GG S++ A  +F +KI  A++V ATM+  G +      + +   
Sbjct: 158 ENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSA 217

Query: 146 SEF-GDVYDLEFGLGSQQPPTTAIIKREFQRKICYHMSPHEDSTLGAMLLRPGPILALKT 205
             F  +   L +G G  +PPT  + ++   + + ++ SP++D  L  + +RP P L    
Sbjct: 218 ERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVP-LGPMM 277

Query: 206 AQFREEEECADKVKRVFIRTMYDRVIKREQQDAMIKRWPPEIVYDM-DTDHSPFFSNPSL 265
            +     E   K +R +++T+ DR +  + Q+ +++   PE V+ +  +DH PFFS P  
Sbjct: 278 EKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQS 337

Query: 266 LFGLLLKAA 273
           L  +LL+ A
Sbjct: 338 LHKILLEIA 345

The following BLAST results are available for this feature:

BLAST of MS003341.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134269.1	2.6e-157	99.28	methylesterase 17 [Momordica charantia]	[more]
XP_038890470.1	2.5e-136	86.38	methylesterase 17 [Benincasa hispida]	[more]
XP_022993026.1	2.4e-131	85.09	methylesterase 17 [Cucurbita maxima]	[more]
XP_022938905.1	2.1e-130	84.00	methylesterase 17 [Cucurbita moschata] >XP_023551105.1 methylesterase 17 [Cucurb...	[more]
KAG6578491.1	2.1e-130	84.00	Methylesterase 17, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS003341.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SG92	4.8e-98	62.98	Methylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=MES17 PE=1 SV=1	[more]
Q2V0W1	1.2e-56	43.60	Pheophorbidase OS=Raphanus sativus OX=3726 GN=PPD PE=1 SV=1	[more]
Q9LVL9	1.9e-54	42.97	Methylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=MES18 PE=2 SV=1	[more]
O23512	4.3e-54	42.91	Probable pheophorbidase OS=Arabidopsis thaliana OX=3702 GN=PPD PE=1 SV=1	[more]
Q9FW03	9.3e-41	37.69	Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES...	[more]

BLAST of MS003341.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYB8	1.3e-157	99.28	methylesterase 17 OS=Momordica charantia OX=3673 GN=LOC111006571 PE=4 SV=1	[more]
A0A6J1JV68	1.2e-131	85.09	methylesterase 17 OS=Cucurbita maxima OX=3661 GN=LOC111489169 PE=4 SV=1	[more]
A0A6J1FL47	1.0e-130	84.00	methylesterase 17 OS=Cucurbita moschata OX=3662 GN=LOC111444979 PE=4 SV=1	[more]
A0A5A7TBF5	2.2e-130	83.33	Methylesterase 17 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001...	[more]
A0A1S3CAT2	2.2e-130	83.33	methylesterase 17 OS=Cucumis melo OX=3656 GN=LOC103498605 PE=4 SV=1	[more]

BLAST of MS003341.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10870.1	3.4e-99	62.98	methyl esterase 17	[more]
AT5G58310.1	1.4e-55	42.97	methyl esterase 18	[more]
AT4G16690.1	3.0e-55	42.91	methyl esterase 16	[more]
AT3G29770.1	6.6e-42	37.69	methyl esterase 11	[more]
AT1G33990.1	4.3e-41	34.14	methyl esterase 14	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 19..274 e-value: 1.5E-54 score: 187.5
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 19..270
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 24..146 e-value: 4.5E-12 score: 46.2
None	No IPR available	PANTHER	PTHR10992:SF1043	METHYLESTERASE 17-LIKE	coord: 10..274
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 10..274

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS003341	MS003341	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS003341.1-cds	MS003341.1-cds-scaffold234:2227712..2227987	CDS
MS003341.1-cds	MS003341.1-cds-scaffold234:2228142..2228276	CDS
MS003341.1-cds	MS003341.1-cds-scaffold234:2230597..2230737	CDS
MS003341.1-cds	MS003341.1-cds-scaffold234:2230852..2231130	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS003341.1	MS003341.1-protein	polypeptide