MS002860.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS002860.1 vs. NCBI nr
Match: KAA0039742.1 (hypothetical protein E6C27_scaffold122G00020 [Cucumis melo var. makuwa] >TYK24754.1 major centromere autoantigen B [Cucumis melo var. makuwa])

HSP 1 Score: 152.5 bits (384), Expect = 4.6e-33
Identity = 134/284 (47.18%), Postives = 162/284 (57.04%), Query Frame = 0

Query: 1   LVVALDKEVVGNENPEEEEAANGEDELKFWEELEEKEFWNGEVDANDVD------DANKP 60
           ++V LDK +VGNENP E EAANGEDE+KFWEELEE+E WNGE+D +D +      D   P
Sbjct: 1   MLVVLDKVLVGNENP-EGEAANGEDEVKFWEELEERELWNGELDKDDANKFEVETDDEVP 60

Query: 61  DEDVPKGFDPKLFVDNEAPNKAEPELPEDEA-VPNRGEIEPEAAADAPGKADP------- 120
           +ED PKG D         PNK EP LPED A VPNR   EPEA A+AP  ADP       
Sbjct: 61  EEDAPKGLD---------PNKPEPVLPEDGADVPNREAPEPEAGAEAPNNADPELAADAA 120

Query: 121 -----GVVVGAEDAPPNKDGA-EAPADGAAPKREKEELPAEE--------AELP------ 180
                 + V AEDAPPNKDGA +APADGAAP +  +E+PAEE         ELP      
Sbjct: 121 VPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPAEEERAPKEGATELPEDGAAP 180

Query: 181 -DGA----APDMDPDELPAAGAAPKREPDELPADGAVPNVKEANPPELPADEGAPNK--G 240
            DGA    AP+ + DE+P   AAP +  +E   +   PN KE NP E   DE  PNK   
Sbjct: 181 NDGAGELPAPNKEADEVPEEEAAPNKGAEEFADEDEAPNPKELNPVE---DEETPNKDEA 240

Query: 241 EGFEEDVAGKGDDPKEVEV-DPNANPELEENGEAEEEEEDWEEE 243
           EG EE+   + D P E E  +     E+EE  E E + ++ EEE
Sbjct: 241 EGAEEEGTLEEDAPVEAEAREKEKGEEVEEEKEEEPKPKEEEEE 271

BLAST of MS002860.1 vs. ExPASy TrEMBL
Match: A0A5A7T8D7 (Major centromere autoantigen B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G003170 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 2.2e-33
Identity = 134/284 (47.18%), Postives = 162/284 (57.04%), Query Frame = 0

Query: 1   LVVALDKEVVGNENPEEEEAANGEDELKFWEELEEKEFWNGEVDANDVD------DANKP 60
           ++V LDK +VGNENP E EAANGEDE+KFWEELEE+E WNGE+D +D +      D   P
Sbjct: 1   MLVVLDKVLVGNENP-EGEAANGEDEVKFWEELEERELWNGELDKDDANKFEVETDDEVP 60

Query: 61  DEDVPKGFDPKLFVDNEAPNKAEPELPEDEA-VPNRGEIEPEAAADAPGKADP------- 120
           +ED PKG D         PNK EP LPED A VPNR   EPEA A+AP  ADP       
Sbjct: 61  EEDAPKGLD---------PNKPEPVLPEDGADVPNREAPEPEAGAEAPNNADPELAADAA 120

Query: 121 -----GVVVGAEDAPPNKDGA-EAPADGAAPKREKEELPAEE--------AELP------ 180
                 + V AEDAPPNKDGA +APADGAAP +  +E+PAEE         ELP      
Sbjct: 121 VPNNGALEVVAEDAPPNKDGADDAPADGAAPNKGADEVPAEEERAPKEGATELPEDGAAP 180

Query: 181 -DGA----APDMDPDELPAAGAAPKREPDELPADGAVPNVKEANPPELPADEGAPNK--G 240
            DGA    AP+ + DE+P   AAP +  +E   +   PN KE NP E   DE  PNK   
Sbjct: 181 NDGAGELPAPNKEADEVPEEEAAPNKGAEEFADEDEAPNPKELNPVE---DEETPNKDEA 240

Query: 241 EGFEEDVAGKGDDPKEVEV-DPNANPELEENGEAEEEEEDWEEE 243
           EG EE+   + D P E E  +     E+EE  E E + ++ EEE
Sbjct: 241 EGAEEEGTLEEDAPVEAEAREKEKGEEVEEEKEEEPKPKEEEEE 271

BLAST of MS002860.1 vs. ExPASy TrEMBL
Match: A0A7J7CCG5 (Uncharacterized protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW18G00307 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 6.3e-04
Identity = 71/177 (40.11%), Postives = 89/177 (50.28%), Query Frame = 0

Query: 67  FVDNEAPNKAEPELPEDEAVPNRGEIEPEAAAD--APGKADPGVVVGAEDAPPNKDGAEA 126
           F D   PNK EPEL  DE VPN+  +EPE AAD  AP K +P     A+D  PNK   E 
Sbjct: 104 FADEGVPNKVEPELSADEGVPNK--VEPELAADEGAPNKVEP--EHAADDGAPNKLEPEL 163

Query: 127 PADGAAPKREKEELPAEEAELPDGAAPDMDPDELPAAGAAPKREPDELPADGAVPNVKEA 186
            AD    K+ + EL  +E  +P    P++  DE+      PK+   EL AD  VP   E 
Sbjct: 164 AADEGVAKKLEPELAVDEV-VPKKLEPELAVDEV-----VPKKFEPELAADEVVPKKFE- 223

Query: 187 NPPELPADEGAPNKGE--GFEEDVAGKGDDPKEVEVDP-NANPELEENGEAEEEEED 239
             PEL ADEG PN G     EE+   +G    E+ +D   A+PE+   G A    ED
Sbjct: 224 --PELAADEGVPNAGAKVSAEENAPKEG---AELAIDVFEADPEV---GVAAPNNED 261