MS002847.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS002847.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	scaffold359: 182736 .. 183497 (+)
Sequence length	762
RNA-Seq Expression	MS002847.1
Synteny	MS002847.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTAGAAGGCAAAGTGGCACTAATCACTGGTGGGGCTAGAGGAATTGGGGAGCACACTGCAAGGCTCTTCTTCAGGCATGGAGCTAAAGTGGTAATTGCAGACATCAAAGACGATTTAGGTCAATCTCTTTCTGACCATCTCTCCTCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAAAGAGAAAGACGTCGAAAACGCGGTCGACACTGCGGTCTCCAGGTACGGAAAGCTTGACATCATGTTCAACAACGCTGGAATCCTCGAAGCTCCAAAGTTCAACATTCTTGAAGATGACTCGTCAAGCTTTCAAAACGTGCTCAACGTCAACCTTCTCGGAGCCTTTCTCGGCACCAAACACGCGGCGCGGGCAATGATTCCTGCGCATCGGGGCAGCATCATCACAACTGCAAGCATCTGCTCGATCATTGGTGGGATCGGCACACATGCATACACAAGCTCAAAGCATGGGGTGTTGGGATTGACCAGAAATGCAGCTGTGGATTTGGGGCGATATGGGATTAGGGTGAATTGTGTATCACCCAATGTAGTGCCCACACAAATGGCTAGGGACTTGTTTAAGCTTGAAGATGGCCAAGAGTTTCCAAATCTATATTCAAATCTCAAAAGTGGAGATGTTTTGAGAGAAGAAGATGTGGCTGAAGCTGCTCTATATTTGGGGAGTGATGCATCAAAATATATAAGTGGGCACAATTTGATTGTTGACGGAGGCTTCACCGTCGTAAATCAAGGTCTT

mRNA sequence

AGATTAGAAGGCAAAGTGGCACTAATCACTGGTGGGGCTAGAGGAATTGGGGAGCACACTGCAAGGCTCTTCTTCAGGCATGGAGCTAAAGTGGTAATTGCAGACATCAAAGACGATTTAGGTCAATCTCTTTCTGACCATCTCTCCTCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAAAGAGAAAGACGTCGAAAACGCGGTCGACACTGCGGTCTCCAGGTACGGAAAGCTTGACATCATGTTCAACAACGCTGGAATCCTCGAAGCTCCAAAGTTCAACATTCTTGAAGATGACTCGTCAAGCTTTCAAAACGTGCTCAACGTCAACCTTCTCGGAGCCTTTCTCGGCACCAAACACGCGGCGCGGGCAATGATTCCTGCGCATCGGGGCAGCATCATCACAACTGCAAGCATCTGCTCGATCATTGGTGGGATCGGCACACATGCATACACAAGCTCAAAGCATGGGGTGTTGGGATTGACCAGAAATGCAGCTGTGGATTTGGGGCGATATGGGATTAGGGTGAATTGTGTATCACCCAATGTAGTGCCCACACAAATGGCTAGGGACTTGTTTAAGCTTGAAGATGGCCAAGAGTTTCCAAATCTATATTCAAATCTCAAAAGTGGAGATGTTTTGAGAGAAGAAGATGTGGCTGAAGCTGCTCTATATTTGGGGAGTGATGCATCAAAATATATAAGTGGGCACAATTTGATTGTTGACGGAGGCTTCACCGTCGTAAATCAAGGTCTT

Coding sequence (CDS)

AGATTAGAAGGCAAAGTGGCACTAATCACTGGTGGGGCTAGAGGAATTGGGGAGCACACTGCAAGGCTCTTCTTCAGGCATGGAGCTAAAGTGGTAATTGCAGACATCAAAGACGATTTAGGTCAATCTCTTTCTGACCATCTCTCCTCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAAAGAGAAAGACGTCGAAAACGCGGTCGACACTGCGGTCTCCAGGTACGGAAAGCTTGACATCATGTTCAACAACGCTGGAATCCTCGAAGCTCCAAAGTTCAACATTCTTGAAGATGACTCGTCAAGCTTTCAAAACGTGCTCAACGTCAACCTTCTCGGAGCCTTTCTCGGCACCAAACACGCGGCGCGGGCAATGATTCCTGCGCATCGGGGCAGCATCATCACAACTGCAAGCATCTGCTCGATCATTGGTGGGATCGGCACACATGCATACACAAGCTCAAAGCATGGGGTGTTGGGATTGACCAGAAATGCAGCTGTGGATTTGGGGCGATATGGGATTAGGGTGAATTGTGTATCACCCAATGTAGTGCCCACACAAATGGCTAGGGACTTGTTTAAGCTTGAAGATGGCCAAGAGTTTCCAAATCTATATTCAAATCTCAAAAGTGGAGATGTTTTGAGAGAAGAAGATGTGGCTGAAGCTGCTCTATATTTGGGGAGTGATGCATCAAAATATATAAGTGGGCACAATTTGATTGTTGACGGAGGCTTCACCGTCGTAAATCAAGGTCTT

Protein sequence

RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDVTKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGTKHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNCVSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHNLIVDGGFTVVNQGL

Homology

[Close]

BLAST of MS002847.1 vs. NCBI nr
Match: XP_022973162.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 411.0 bits (1055), Expect = 7.5e-111
Identity = 200/254 (78.74%), Postives = 226/254 (88.98%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFF+HGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKD+ENAV+TA+++YGKLDIM NNAGI    KFN LE++SS FQNVLNVNLLG FLGT
Sbjct: 72  TKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAARAMIPA +GSII TAS+CS+IGGIG + YTSSKHGVLGL +NA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP+VVP+QM RDLFKLEDG EFPN+YS+ K GD+LR ED+AEAALYL SDAS+++SGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFTVVNQGL 255
           LIVDGGFT  NQGL
Sbjct: 252 LIVDGGFTSANQGL 265

[Close]

BLAST of MS002847.1 vs. NCBI nr
Match: XP_038895162.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 409.8 bits (1052), Expect = 1.7e-110
Identity = 208/255 (81.57%), Postives = 229/255 (89.80%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVALITGGARGIGE+TARLFF+HGAKVVIADI+DDLG SLS+H+ SSS SFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIEDDLGHSLSNHIGSSSFSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKDVENAVD+A+S YGKLDIMFNNA IL APKFNILE++SS FQ VLNVNLLGAFLGT
Sbjct: 72  TKEKDVENAVDSALSDYGKLDIMFNNAAILGAPKFNILENESSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAAR MIPA RGSIITT S+ S IGGIG HA TSSKHGV+GL +NAAVDLGRYGIRVNC
Sbjct: 132 KHAARVMIPAGRGSIITTGSVISSIGGIGPHACTSSKHGVVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSG-DVLREEDVAEAALYLGSDASKYISGH 240
           VSP  V T+M R+LFK++DG E P+LY NLK G DV++EEDVAEAALYL SDASKY+SGH
Sbjct: 192 VSPYGVHTKMTRELFKVKDGDEIPSLYWNLKCGEDVVKEEDVAEAALYLASDASKYVSGH 251

Query: 241 NLIVDGGFTVVNQGL 255
           NL+VDGGFTVVNQGL
Sbjct: 252 NLVVDGGFTVVNQGL 266

[Close]

BLAST of MS002847.1 vs. NCBI nr
Match: XP_022142634.1 (secoisolariciresinol dehydrogenase-like [Momordica charantia])

HSP 1 Score: 409.5 bits (1051), Expect = 2.2e-110
Identity = 202/254 (79.53%), Postives = 224/254 (88.19%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGE+TARLF +HGAKVVIADI DDLGQSLS+HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKDVEN VDT VS+YGKLDIMFNNA IL AP +NILE+DSS FQ VLNVNL GAFLGT
Sbjct: 72  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAARAMIPA RGSIIT AS  + +GGI  HA TSSKHGVLGL +NAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP  VPTQ+ RDL +L+DG +FPN+Y NLK+ D + EEDVAEAALYLGSDA+KY+SGHN
Sbjct: 192 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 251

Query: 241 LIVDGGFTVVNQGL 255
           L+VDGGF+V+N GL
Sbjct: 252 LVVDGGFSVLNSGL 265

[Close]

BLAST of MS002847.1 vs. NCBI nr
Match: XP_022142407.1 (momilactone A synthase-like [Momordica charantia])

HSP 1 Score: 408.7 bits (1049), Expect = 3.7e-110
Identity = 205/252 (81.35%), Postives = 226/252 (89.68%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVALITGGARGIGE+TARLFF+HGAKVVIADI+DDLG SLS++L  SSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T+E DV NAVDT VS+YGKLDIMFNNAGI    KFNILE++S  FQ VLNVNLLGAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAARAM+PA RGSII TAS CS+IGG   HAYTSSKHG++GL +NAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP+VVPTQM RDLFKLEDG EFPN YS+LK G +L EED+AEAALYLGSDASK++SGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LIVDGGFTVVNQ 253
           LIVDGGFTVVN+
Sbjct: 252 LIVDGGFTVVNR 263

[Close]

BLAST of MS002847.1 vs. NCBI nr
Match: XP_038895003.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 407.5 bits (1046), Expect = 8.3e-110
Identity = 204/256 (79.69%), Postives = 227/256 (88.67%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSL-SDHLSSSSSSFVHCD 60
           RLEGKVA+ITGGARGIGEHTARLF +HGAKVVIADI+DDLG +L +D+L SSSSSFVHCD
Sbjct: 12  RLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCD 71

Query: 61  VTKEKDVENAVDTAVSRYGKLDIMFNNAGIL-EAPKFNILEDDSSSFQNVLNVNLLGAFL 120
           VTKEKDVENA+DTAVS+YGKLDIMFNNAG+  EA  F+IL+DD  +FQ V+NVNL+GAFL
Sbjct: 72  VTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFL 131

Query: 121 GTKHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRV 180
           GTKHAAR M P  RGSI+TTASICS+IGGIGTHAYTSSKHGVLGL RN AVDLGRYGIRV
Sbjct: 132 GTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRYGIRV 191

Query: 181 NCVSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISG 240
           NCVSPNVVPT+M R LFKL++G EFP+ Y NLK GD+LREEDV EA +YLGSD SK +SG
Sbjct: 192 NCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKCVSG 251

Query: 241 HNLIVDGGFTVVNQGL 255
            NLIVDGGFTVVNQ L
Sbjct: 252 LNLIVDGGFTVVNQAL 267

[Close]

BLAST of MS002847.1 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.6e-76
Identity = 144/254 (56.69%), Postives = 191/254 (75.20%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVALITGGA GIGE TA+LF +HGAKV IAD++D+LG S+ + + +S+S+++HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T E  V+NAVD  VS YGKLDIMF+NAGI +  +  I++++ + F+ V +VN+ G FL  
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCM 133

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAAR MIPA  G+II+TAS+ S +GG  +HAY  SKH VLGLTRN AV+LG++GIRVNC
Sbjct: 134 KHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNC 193

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYS---NLKSGDVLREEDVAEAALYLGSDASKYIS 240
           +SP  +PT + +    +++ +EF N+ +   NLK G     EDVA AALYL SD +KY+S
Sbjct: 194 LSPFGLPTALGKKFSGIKNEEEFENVINFAGNLK-GPKFNVEDVANAALYLASDEAKYVS 253

Query: 241 GHNLIVDGGFTVVN 252
           GHNL +DGGF+V N
Sbjct: 254 GHNLFIDGGFSVCN 266

[Close]

BLAST of MS002847.1 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 9.9e-74
Identity = 144/253 (56.92%), Postives = 181/253 (71.54%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           +L GKVA+ITGGA GIG  TARLF +HGA+VV+ADI+D+LG SL   L   +SS+VHCDV
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDV 73

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T E DV  AVD AV+R+GKLD+MFNNAG+   P F + E     F+ VL VNL+G FLGT
Sbjct: 74  TNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLGT 133

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAAR M PA RGSII+TAS+ S + G  +HAYT+SKH ++G T NAA +LGR+GIRVNC
Sbjct: 134 KHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRVNC 193

Query: 181 VSPNVVPTQMARDLFKLEDG--QEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISG 240
           VSP  V T +AR    ++D   +      +NLK    L+ +D+A AAL+L SD  +Y+SG
Sbjct: 194 VSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYVSG 253

Query: 241 HNLIVDGGFTVVN 252
            NL VDGG +VVN
Sbjct: 254 QNLRVDGGLSVVN 266

[Close]

BLAST of MS002847.1 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.1e-71
Identity = 139/255 (54.51%), Postives = 184/255 (72.16%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSS-SSFVHCD 60
           RL+ KVA+ITGGA GIGE TA+LF R+GAKVVIADI DD GQ + +++ S    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLG 120
           VTK++DV N VDT ++++GKLDIMF N G+L    ++ILE  +  F+ V+++N+ GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMIPAHRGSIITTASICSIIGGIG-THAYTSSKHGVLGLTRNAAVDLGRYGIRV 180
            KHAAR MIPA +GSI+ TASI S   G G +H YT++KH VLGLT +   +LG YGIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 181 NCVSPNVVPTQMARDLFKLEDG--QEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYI 240
           NCVSP +V + +  D+F ++    +E  +  +NLK G +LR EDVA+A  YL  D SKY+
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLK-GTLLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLIVDGGFTVVN 252
           SG NL++DGG+T  N
Sbjct: 253 SGLNLVIDGGYTRTN 266

[Close]

BLAST of MS002847.1 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 6.0e-71
Identity = 139/255 (54.51%), Postives = 185/255 (72.55%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSS-SSFVHCD 60
           RL+ KVA+ITGGA GIGE TA+LF R+GAKVVIADI DD GQ + +++ S    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLG 120
           VTK++DV N VDT ++++GKLDIMF N G+L    ++ILE  +  F+ V+++N+ GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMIPAHRGSIITTASICSIIGGIG-THAYTSSKHGVLGLTRNAAVDLGRYGIRV 180
            KHAAR MIPA +GSI+ TASI S   G G +H YT++KH VLGLT +   +LG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 181 NCVSPNVVPTQMARDLFKLEDG--QEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYI 240
           NCVSP VV + +  D+F ++    +E  +  +NLK G +LR EDVA+A  YL  D SKY+
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLK-GILLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLIVDGGFTVVN 252
           SG NL++DGG+T  N
Sbjct: 253 SGLNLVIDGGYTRTN 266

[Close]

BLAST of MS002847.1 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.3e-69
Identity = 141/266 (53.01%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKD----DLGQSLSDHLSSSSSSFV 60
           RLEGKVA+ITGGA GIG+ T  LF RHGA VVIAD+ +     L +SLS H +S   +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPK--FNILEDDSSSFQNVLNVNLL 120
            CDV+ E DVEN V+  V+RYG+LDI+FNNAG+L   K   +IL+ D+  F +V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GAFLGTKHAARAMIP-AHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGR 180
           G  LG KH ARAMI    +G II+TAS+  ++GG+G HAYT+SKH ++GLT+NAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 YGIRVNCVSPNVVPTQMARDLFK-----------LEDGQEFPNLYSNLKSGDVLREEDVA 240
           YGIRVNC+SP  V T M  + ++           +E+ +EF    +NLK G+ LR  D+A
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDIA 270

Query: 241 EAALYLGSDASKYISGHNLIVDGGFT 249
           EAALYL SD SKY++GHNL+VDGG T
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of MS002847.1 vs. ExPASy TrEMBL
Match: A0A6J1I6S5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471670 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 3.6e-111
Identity = 200/254 (78.74%), Postives = 226/254 (88.98%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFF+HGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKD+ENAV+TA+++YGKLDIM NNAGI    KFN LE++SS FQNVLNVNLLG FLGT
Sbjct: 72  TKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAARAMIPA +GSII TAS+CS+IGGIG + YTSSKHGVLGL +NA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP+VVP+QM RDLFKLEDG EFPN+YS+ K GD+LR ED+AEAALYL SDAS+++SGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFTVVNQGL 255
           LIVDGGFT  NQGL
Sbjct: 252 LIVDGGFTSANQGL 265

[Close]

BLAST of MS002847.1 vs. ExPASy TrEMBL
Match: A0A6J1CM16 (secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111012697 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.1e-110
Identity = 202/254 (79.53%), Postives = 224/254 (88.19%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGE+TARLF +HGAKVVIADI DDLGQSLS+HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKDVEN VDT VS+YGKLDIMFNNA IL AP +NILE+DSS FQ VLNVNL GAFLGT
Sbjct: 72  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAARAMIPA RGSIIT AS  + +GGI  HA TSSKHGVLGL +NAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP  VPTQ+ RDL +L+DG +FPN+Y NLK+ D + EEDVAEAALYLGSDA+KY+SGHN
Sbjct: 192 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 251

Query: 241 LIVDGGFTVVNQGL 255
           L+VDGGF+V+N GL
Sbjct: 252 LVVDGGFSVLNSGL 265

[Close]

BLAST of MS002847.1 vs. ExPASy TrEMBL
Match: A0A6J1CMN7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.8e-110
Identity = 205/252 (81.35%), Postives = 226/252 (89.68%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVALITGGARGIGE+TARLFF+HGAKVVIADI+DDLG SLS++L  SSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T+E DV NAVDT VS+YGKLDIMFNNAGI    KFNILE++S  FQ VLNVNLLGAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAARAM+PA RGSII TAS CS+IGG   HAYTSSKHG++GL +NAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP+VVPTQM RDLFKLEDG EFPN YS+LK G +L EED+AEAALYLGSDASK++SGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LIVDGGFTVVNQ 253
           LIVDGGFTVVN+
Sbjct: 252 LIVDGGFTVVNR 263

[Close]

BLAST of MS002847.1 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 200/254 (78.74%), Postives = 225/254 (88.58%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFF+HGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKD+ENAV+TA+S+YGKLDIM NNAGI    KFNILE++ S FQ VLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAA+AMIPA +GSII  AS CSIIGGIG +AYTSSKHG+LGL +NA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP+VVP+QM RDLFKLEDG EFPN+YS+ K GD+LR ED+AEAALYL SDAS+++SGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFTVVNQGL 255
           LIVDGGFT  NQGL
Sbjct: 252 LIVDGGFTSANQGL 265

[Close]

BLAST of MS002847.1 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 2.0e-109
Identity = 200/254 (78.74%), Postives = 225/254 (88.58%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFF+HGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           TKEKD+ENAV+TA+S+YGKLDIM NNAGI    KFNILE++ S FQ VLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVNC 180
           KHAA+AMIPA +GSII  AS CSIIGGIG +AYTSSKHG+LGL +NA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPNVVPTQMARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISGHN 240
           VSP+VVP+QM RDLFKLEDG EFPN+YS+ K GD+LR ED+AEAALYL SDAS+++SGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFTVVNQGL 255
           LIVDGGFT  NQGL
Sbjct: 252 LIVDGGFTSANQGL 265

[Close]

BLAST of MS002847.1 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.1e-70
Identity = 141/266 (53.01%), Postives = 186/266 (69.92%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKD----DLGQSLSDHLSSSSSSFV 60
           RLEGKVA+ITGGA GIG+ T  LF RHGA VVIAD+ +     L +SLS H +S   +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPK--FNILEDDSSSFQNVLNVNLL 120
            CDV+ E DVEN V+  V+RYG+LDI+FNNAG+L   K   +IL+ D+  F +V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GAFLGTKHAARAMIP-AHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGR 180
           G  LG KH ARAMI    +G II+TAS+  ++GG+G HAYT+SKH ++GLT+NAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 YGIRVNCVSPNVVPTQMARDLFK-----------LEDGQEFPNLYSNLKSGDVLREEDVA 240
           YGIRVNC+SP  V T M  + ++           +E+ +EF    +NLK G+ LR  D+A
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDIA 270

Query: 241 EAALYLGSDASKYISGHNLIVDGGFT 249
           EAALYL SD SKY++GHNL+VDGG T
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of MS002847.1 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 241.9 bits (616), Expect = 5.6e-64
Identity = 134/263 (50.95%), Postives = 170/263 (64.64%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLG----QSLSDHLSSSSSSFV 60
           RL GKVALITGGA GIGE   RLF +HGAKV I D++DDLG    +SL    S  ++ F+
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 61  HCDVTKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGA 120
           H DV  E D+ NAVD AV  +G LDI+ NNAG+  AP  +I     S F+   +VN+ GA
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 121 FLGTKHAARAMIPAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGI 180
           FL  KHAAR MIP  +GSI++  S+  ++GG+G H+Y  SKH VLGLTR+ A +LG++GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 181 RVNCVSPNVVPTQMARDLFKLEDGQE--------FPNLYSNLKSGDVLREEDVAEAALYL 240
           RVNCVSP  V T++A      E+  E        F    +NLK G  L  +DVA A L+L
Sbjct: 197 RVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLK-GVELTVDDVANAVLFL 256

Query: 241 GSDASKYISGHNLIVDGGFTVVN 252
            SD S+YISG NL++DGGFT  N
Sbjct: 257 ASDDSRYISGDNLMIDGGFTCTN 278

[Close]

BLAST of MS002847.1 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 3.4e-61
Identity = 131/253 (51.78%), Postives = 175/253 (69.17%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RL+GK+A+ITGGA GIG    RLF  HGAKVVI DI+++LGQ+L+  +    +SF  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T E DVENAV   V ++GKLD++F+NAG+LEA   ++L+ D  +F   + VN+ GA    
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVLEA-FGSVLDLDLEAFDRTMAVNVRGAAAFI 124

Query: 121 KHAARAMIPA-HRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVN 180
           KHAAR+M+ +  RGSI+ T SI + IGG G H+YT+SKH +LGL R+A   LG+YGIRVN
Sbjct: 125 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 184

Query: 181 CVSPNVVPTQM--ARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYIS 240
            V+P  V T M  A +   ++  +E+     NLK G VL+   +AEAAL+L SD S YIS
Sbjct: 185 GVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLK-GVVLKARHIAEAALFLASDDSVYIS 244

Query: 241 GHNLIVDGGFTVV 251
           G NL+VDGGF+VV
Sbjct: 245 GQNLVVDGGFSVV 255

[Close]

BLAST of MS002847.1 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.4e-61
Identity = 128/252 (50.79%), Postives = 170/252 (67.46%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RL+GK+A+ITGGA GIG    RLF  HGAKVVI D +++LGQ+++  +    +SF  CDV
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T EK+VENAV   V +YGKLD++F+NAG++E P  + L+ +   F   + VN+ GA    
Sbjct: 65  TNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQPG-SFLDLNLEQFDRTMAVNVRGAAAFI 124

Query: 121 KHAARAMI-PAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+ T S+ S IGG G HAYT+SKH +LGL ++A   LG+YGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRVN 184

Query: 181 CVSPNVVPTQM-ARDLFKLEDGQEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYISG 240
            V+P  V T + +RD   +   +E+      LK G VL+   VAEAAL+L SD S Y+SG
Sbjct: 185 GVAPYAVATAINSRDEETVRMVEEYSAATGILK-GVVLKARHVAEAALFLASDDSAYVSG 244

Query: 241 HNLIVDGGFTVV 251
            NL VDGG++VV
Sbjct: 245 QNLAVDGGYSVV 254

[Close]

BLAST of MS002847.1 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 231.9 bits (590), Expect = 5.8e-61
Identity = 123/253 (48.62%), Postives = 173/253 (68.38%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEHTARLFFRHGAKVVIADIKDDLGQSLSDHLSSSSSSFVHCDV 60
           RL+GK+ +ITGGA GIG  + RLF  HGA+VVI D++D+LGQ+++  +    +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 61  TKEKDVENAVDTAVSRYGKLDIMFNNAGILEAPKFNILEDDSSSFQNVLNVNLLGAFLGT 120
           T E +VENAV   V +YGKLD++F+NAG++E P  +IL+ + +     + +NL G     
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVIE-PFVSILDLNLNELDRTIAINLRGTAAFI 124

Query: 121 KHAARAMI-PAHRGSIITTASICSIIGGIGTHAYTSSKHGVLGLTRNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+ T S+ + I G   H YT+SKHG+LGL ++A+  LG+YGIRVN
Sbjct: 125 KHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRVN 184

Query: 181 CVSPNVVPTQMARDLFKLEDG--QEFPNLYSNLKSGDVLREEDVAEAALYLGSDASKYIS 240
            V+P  V T +  + FK+E    ++  +  +NLK G VL+   VAEAAL+L SD S Y+S
Sbjct: 185 GVAPFGVATPLVCNGFKMEPNVVEQNTSASANLK-GIVLKARHVAEAALFLASDESAYVS 244

Query: 241 GHNLIVDGGFTVV 251
           G NL VDGG++VV
Sbjct: 245 GQNLAVDGGYSVV 255

The following BLAST results are available for this feature:

BLAST of MS002847.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022973162.1	7.5e-111	78.74	secoisolariciresinol dehydrogenase-like [Cucurbita maxima]	[more]
XP_038895162.1	1.7e-110	81.57	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]
XP_022142634.1	2.2e-110	79.53	secoisolariciresinol dehydrogenase-like [Momordica charantia]	[more]
XP_022142407.1	3.7e-110	81.35	momilactone A synthase-like [Momordica charantia]	[more]
XP_038895003.1	8.3e-110	79.69	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]

BLAST of MS002847.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	1.6e-76	56.69	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	9.9e-74	56.92	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q94KL8	2.1e-71	54.51	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	6.0e-71	54.51	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q9SCU0	4.3e-69	53.01	Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ...	[more]

BLAST of MS002847.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I6S5	3.6e-111	78.74	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A6J1CM16	1.1e-110	79.53	secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1CMN7	1.8e-110	81.35	momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 ...	[more]
A0A6J1EID0	2.0e-109	78.74	secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EI14	2.0e-109	78.74	secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]

BLAST of MS002847.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	3.1e-70	53.01	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	5.6e-64	50.95	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	3.4e-61	51.78	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	4.4e-61	50.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	5.8e-61	48.62	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 174..191 score: 41.43 coord: 153..172 score: 39.29 coord: 211..231 score: 28.23 coord: 6..23 score: 50.34 coord: 127..143 score: 31.02 coord: 78..89 score: 58.12
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 78..89 score: 51.1 coord: 133..141 score: 40.65 coord: 153..172 score: 35.44
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 11..248 e-value: 2.5E-67 score: 226.8
None	No IPR available	GENE3D	3.40.50.720		coord: 1..253 e-value: 2.1E-79 score: 268.4
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 8..202 e-value: 1.0E-17 score: 62.1
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 2..250 e-value: 3.6E-19 score: 66.7
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..253
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 1..253
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 140..168
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..250

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS002847	MS002847	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS002847.1-cds	MS002847.1-cds-scaffold359:182736..183497	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS002847.1	MS002847.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity