MS001235.1 (mRNA) Bitter gourd (TR) v1

Overview


Name	MS001235.1
Type	mRNA
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	calmodulin-like protein 2
Location	scaffold36: 2548312 .. 2548851 (+)
Sequence length	540
RNA-Seq Expression	MS001235.1
Synteny	MS001235.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCATCTCCAGATTCATTGGGGACTTTTTACAAACACTTGGCGGGGGGTTCCGGGCGGCCGCACAATCCGACGCCCCACCGAGCATCTCCCAGCCCCGCGCCGCCGCCTACGACGACGCCCTGCTGCACGCCCTGATCACGGTGTTCGGGATGCGGGAAAACGGGAGGATCAAGAAGGAGAAAGCGAAGGGGGTGGTGGAGAAGCTGGGGCTGATACAGGGCGAGGAGGAGAAGGCGAGGTTCGATCTGCCGCCGGGGGAGGGCGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGGGAGATGGCGGAGGAGGCGGCGGCGGGGAAGCAGAGCGAGCTGCTGAGGGAAGCGTTCAAGATATTCGATGTGGATGGAGATGGGTTCATAGATACTGTGGAGTTGAAGAGGGTGCTGCAATGTTTGGGGCTGGATAAAGGGTGGGGAATTAGGGAGATCGAGAAGATGGTTAGAGTTGTGGATGTCAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTCAATTAATGATGGGTGTCAAA

mRNA sequence

ATGGGCATCTCCAGATTCATTGGGGACTTTTTACAAACACTTGGCGGGGGGTTCCGGGCGGCCGCACAATCCGACGCCCCACCGAGCATCTCCCAGCCCCGCGCCGCCGCCTACGACGACGCCCTGCTGCACGCCCTGATCACGGTGTTCGGGATGCGGGAAAACGGGAGGATCAAGAAGGAGAAAGCGAAGGGGGTGGTGGAGAAGCTGGGGCTGATACAGGGCGAGGAGGAGAAGGCGAGGTTCGATCTGCCGCCGGGGGAGGGCGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGGGAGATGGCGGAGGAGGCGGCGGCGGGGAAGCAGAGCGAGCTGCTGAGGGAAGCGTTCAAGATATTCGATGTGGATGGAGATGGGTTCATAGATACTGTGGAGTTGAAGAGGGTGCTGCAATGTTTGGGGCTGGATAAAGGGTGGGGAATTAGGGAGATCGAGAAGATGGTTAGAGTTGTGGATGTCAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTCAATTAATGATGGGTGTCAAA

Coding sequence (CDS)

ATGGGCATCTCCAGATTCATTGGGGACTTTTTACAAACACTTGGCGGGGGGTTCCGGGCGGCCGCACAATCCGACGCCCCACCGAGCATCTCCCAGCCCCGCGCCGCCGCCTACGACGACGCCCTGCTGCACGCCCTGATCACGGTGTTCGGGATGCGGGAAAACGGGAGGATCAAGAAGGAGAAAGCGAAGGGGGTGGTGGAGAAGCTGGGGCTGATACAGGGCGAGGAGGAGAAGGCGAGGTTCGATCTGCCGCCGGGGGAGGGCGGTGATGAGGTGGCGGTGGAGGAGGTGATCGGGGAGATGGCGGAGGAGGCGGCGGCGGGGAAGCAGAGCGAGCTGCTGAGGGAAGCGTTCAAGATATTCGATGTGGATGGAGATGGGTTCATAGATACTGTGGAGTTGAAGAGGGTGCTGCAATGTTTGGGGCTGGATAAAGGGTGGGGAATTAGGGAGATCGAGAAGATGGTTAGAGTTGTGGATGTCAATTTGGATGGGAAAGTTGATTTTGGTGAGTTTCAATTAATGATGGGTGTCAAA

Protein sequence

MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKKEKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK

Homology

[Close]

BLAST of MS001235.1 vs. NCBI nr
Match: XP_022132261.1 (calmodulin-like protein 2 [Momordica charantia])

HSP 1 Score: 351.7 bits (901), Expect = 3.8e-93
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK
Sbjct: 72  MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 131

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK
Sbjct: 132 EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 191

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK
Sbjct: 192 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 251

[Close]

BLAST of MS001235.1 vs. NCBI nr
Match: XP_022951438.1 (calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265.4 bits (677), Expect = 3.6e-67
Identity = 143/180 (79.44%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GG  R AA S A      P    +DD LL ALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI+ EEEK  F+LP   GGDEVAVEEVIGEM EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMV VVDVNLDGKVDFGEF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVK 176

[Close]

BLAST of MS001235.1 vs. NCBI nr
Match: XP_023002517.1 (calmodulin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 263.8 bits (673), Expect = 1.0e-66
Identity = 143/180 (79.44%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GG  R AA S A      P    +DD LL ALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSR-AADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI+ EEEK  F+LP   GGDEVAVEEVIGEM EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMV VVDVNLDGKVDFGEF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVK 176

[Close]

BLAST of MS001235.1 vs. NCBI nr
Match: XP_038886166.1 (calmodulin-like protein 2 [Benincasa hispida])

HSP 1 Score: 263.8 bits (673), Expect = 1.0e-66
Identity = 144/181 (79.56%), Postives = 152/181 (83.98%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GG  R+AA+S      ++P    YDD LL ALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDL-PPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAF 120
           EKAKGVVEKLGLIQ EEEKARF+L    EGGDEVAVEEVIGE  EE   GK++ELL EAF
Sbjct: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGE--EE---GKRNELLYEAF 120

Query: 121 KIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGV 180
           KIFDVDGDGFIDTVELKRVL CLGLDKGWGIREIEKMV VVDVNLDGKVDF EF+LMMGV
Sbjct: 121 KIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGV 176

[Close]

BLAST of MS001235.1 vs. NCBI nr
Match: KAG6585405.1 (putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020321.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.1 bits (671), Expect = 1.8e-66
Identity = 142/180 (78.89%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ G  R AA S A      P    +DD LL ALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVSGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI+ EEEK  F+LP   GGDEVAVEEVIGEM EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMV VVDVNLDGKVDFGEF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVK 176

[Close]

BLAST of MS001235.1 vs. ExPASy Swiss-Prot
Match: Q84UL5 (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 GN=CML32 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-13
Identity = 59/179 (32.96%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 26  APPSISQPRAAA--------YDDALLHALITVFGMRENGRIKKEKAKGVVEKLGLI---Q 85
           A PS+++  A+A         +   L  +  +F    +G I  ++    ++ LGL+    
Sbjct: 10  AKPSLAKKTASASFRLRNGSLNAVRLRRVFDLFDRNGDGEITVDELAQALDALGLVADRD 69

Query: 86  GEEEKARFDLPPGEGG----DEVAVEEVIGE-----MAEEAAAGK-------QSELLREA 145
           G        +P G  G    D  A+   +G+     +   AAAG+       + E +REA
Sbjct: 70  GLAATVSAYVPEGAAGLRFEDFDALHRALGDALFGSLDGAAAAGEPGGGGGDEEEEMREA 129

Query: 146 FKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMM 178
           FK+FDVDGDGFI   EL+ VL+ LGL +   +  + +M+  VD N DG+VDFGEF+ MM
Sbjct: 130 FKVFDVDGDGFISASELQEVLKKLGLPEAGSLATVREMICNVDRNSDGRVDFGEFKSMM 188

[Close]

BLAST of MS001235.1 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.1e-13
Identity = 47/140 (33.57%), Postives = 74/140 (52.86%), Query Frame = 0


Query: 39  DDALLHALITVFGMRENGRIKKEKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEV 98
           D   L  +  +F    +G+I K + K   + +G++  E E           GD     + 
Sbjct: 2   DRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDE 61

Query: 99  IGEMAEEAAAGKQSEL-LREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMV 158
            G + +E    K+ E  +REAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+
Sbjct: 62  FGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMI 121

Query: 159 RVVDVNLDGKVDFGEFQLMM 178
             VDV+ DG V+F EF+ MM
Sbjct: 122 SKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of MS001235.1 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.9e-13
Identity = 54/155 (34.84%), Postives = 80/155 (51.61%), Query Frame = 0


Query: 34  RAAAYDDALLHALITVFGMRENGRIKKEKAKGVVEKLGL-IQGEEEKARFD--LPPGEGG 93
           R+ + +   LH +  +F    +G I  E+    + +LGL     + K+  D  + P + G
Sbjct: 20  RSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTG 79

Query: 94  ----DEVAVEEVIGEM----AEEAAAGKQSELLREAFKIFDVDGDGFIDTVELKRVLQCL 153
               D  A+ + + E           G     L EAF +FD DGDGFI  VEL++VL+ L
Sbjct: 80  LRFDDFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKL 139

Query: 154 GLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMM 178
           GL +   I ++EKM+  VD N DG+VDF EF+ MM
Sbjct: 140 GLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of MS001235.1 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.1e-13
Identity = 59/180 (32.78%), Postives = 89/180 (49.44%), Query Frame = 0


Query: 21  AAQSDAPPSISQP-------RAAAYDDALLHALITVFGMRENGRIKKEKAKGVVEKLGL- 80
           AA   A PS+S+        R  + +   L  +  +F    +G I  ++    ++ LGL 
Sbjct: 3   AAGVAAKPSLSRKPSPSFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASALDALGLG 62

Query: 81  --IQGEEEKARFDLPPGEG----GDEVAVEEVIGEM----AEEAAAGKQSE-----LLRE 140
               G E      +P G      GD  A+   +G+      EE   GKQ E      ++E
Sbjct: 63  ADRAGLEATVGGYIPAGAAGLRFGDFEALHRALGDALFGPVEEEEPGKQGEDDDEGDMKE 122

Query: 141 AFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMM 178
           AF++FD DGDGFI   EL+ VL+ LGL +   +  +++M+  VD + DG+VDFGEF+ MM
Sbjct: 123 AFRVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGRVDFGEFKCMM 182

[Close]

BLAST of MS001235.1 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 48/143 (33.57%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 39  DDALLHALITVFGMRENGRIKKEKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEV 98
           D   L+ +  +F    +G+I  ++     + LG+I  E+E  +        GD     E 
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 99  IGEMAEEAAAGKQSEL----LREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIE 158
            GE+ +      + E+    ++EAF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 159 KMVRVVDVNLDGKVDFGEFQLMM 178
           KM+  VDV+ DG+V++ EF+ MM
Sbjct: 122 KMIMQVDVDGDGRVNYMEFRQMM 144

[Close]

BLAST of MS001235.1 vs. ExPASy TrEMBL
Match: A0A6J1BRS1 (calmodulin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111005158 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 1.9e-93
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK
Sbjct: 72  MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 131

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK
Sbjct: 132 EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 191

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK
Sbjct: 192 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 251

[Close]

BLAST of MS001235.1 vs. ExPASy TrEMBL
Match: A0A6J1GHP6 (calmodulin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111454254 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.7e-67
Identity = 143/180 (79.44%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GG  R AA S A      P    +DD LL ALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSR-AADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI+ EEEK  F+LP   GGDEVAVEEVIGEM EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMV VVDVNLDGKVDFGEF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVK 176

[Close]

BLAST of MS001235.1 vs. ExPASy TrEMBL
Match: A0A6J1KJR1 (calmodulin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111496334 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.1e-67
Identity = 143/180 (79.44%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GG  R AA S A      P    +DD LL ALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSR-AADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI+ EEEK  F+LP   GGDEVAVEEVIGEM EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEE---GKRNELLWEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMV VVDVNLDGKVDFGEF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVK 176

[Close]

BLAST of MS001235.1 vs. ExPASy TrEMBL
Match: A0A5A7VBM3 (Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003420 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.3e-65
Identity = 141/180 (78.33%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGG  RAAA+S      S+P    YDD LL ALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI   EEK +F+L  GEGGDEVAVEEVIGE  EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIGE--EE---GKRNELLYEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDT+ELKRV+ CLGLDKGWGIREIEKMV VVDVNLDGKVDF EF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVK 172

[Close]

BLAST of MS001235.1 vs. ExPASy TrEMBL
Match: A0A1S3BBS0 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.3e-65
Identity = 141/180 (78.33%), Postives = 150/180 (83.33%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGGFRAAAQSDAPPSISQPRAAAYDDALLHALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGG  RAAA+S      S+P    YDD LL ALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEVIGEMAEEAAAGKQSELLREAFK 120
           EKAKGVVEKLGLI   EEK +F+L  GEGGDEVAVEEVIGE  EE   GK++ELL EAFK
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIGE--EE---GKRNELLYEAFK 120

Query: 121 IFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMMGVK 180
           IFDVDGDGFIDT+ELKRV+ CLGLDKGWGIREIEKMV VVDVNLDGKVDF EF+LMMGVK
Sbjct: 121 IFDVDGDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVK 172

[Close]

BLAST of MS001235.1 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 76.3 bits (186), Expect = 2.9e-14
Identity = 47/140 (33.57%), Postives = 74/140 (52.86%), Query Frame = 0


Query: 39  DDALLHALITVFGMRENGRIKKEKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEV 98
           D   L  +  +F    +G+I K + K   + +G++  E E           GD     + 
Sbjct: 2   DRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDE 61

Query: 99  IGEMAEEAAAGKQSEL-LREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMV 158
            G + +E    K+ E  +REAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+
Sbjct: 62  FGSLYQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMI 121

Query: 159 RVVDVNLDGKVDFGEFQLMM 178
             VDV+ DG V+F EF+ MM
Sbjct: 122 SKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of MS001235.1 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 75.5 bits (184), Expect = 4.9e-14
Identity = 54/155 (34.84%), Postives = 80/155 (51.61%), Query Frame = 0


Query: 34  RAAAYDDALLHALITVFGMRENGRIKKEKAKGVVEKLGL-IQGEEEKARFD--LPPGEGG 93
           R+ + +   LH +  +F    +G I  E+    + +LGL     + K+  D  + P + G
Sbjct: 20  RSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTG 79

Query: 94  ----DEVAVEEVIGEM----AEEAAAGKQSELLREAFKIFDVDGDGFIDTVELKRVLQCL 153
               D  A+ + + E           G     L EAF +FD DGDGFI  VEL++VL+ L
Sbjct: 80  LRFDDFAALHKTLDESFFGGEGSCCDGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKL 139

Query: 154 GLDKGWGIREIEKMVRVVDVNLDGKVDFGEFQLMM 178
           GL +   I ++EKM+  VD N DG+VDF EF+ MM
Sbjct: 140 GLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

[Close]

BLAST of MS001235.1 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 74.7 bits (182), Expect = 8.4e-14
Identity = 48/143 (33.57%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 39  DDALLHALITVFGMRENGRIKKEKAKGVVEKLGLIQGEEEKARFDLPPGEGGDEVAVEEV 98
           D   L+ +  +F    +G+I  ++     + LG+I  E+E  +        GD     E 
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 99  IGEMAEEAAAGKQSEL----LREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIE 158
            GE+ +      + E+    ++EAF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 159 KMVRVVDVNLDGKVDFGEFQLMM 178
           KM+  VDV+ DG+V++ EF+ MM
Sbjct: 122 KMIMQVDVDGDGRVNYMEFRQMM 144

[Close]

BLAST of MS001235.1 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 71.2 bits (173), Expect = 9.3e-13
Identity = 38/79 (48.10%), Postives = 54/79 (68.35%), Query Frame = 0


Query: 100 GEMAEEAAAGKQSEL-LREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIEKMVR 159
           GE  ++ ++  ++E  L EAFK+FD +GDGFI   EL+ VL+ LGL +G  +  +EKM+ 
Sbjct: 105 GENEDDPSSAAENESDLAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIV 164

Query: 160 VVDVNLDGKVDFGEFQLMM 178
            VD N DG+VDF EF+ MM
Sbjct: 165 SVDRNQDGRVDFFEFKNMM 183

[Close]

BLAST of MS001235.1 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-12
Identity = 48/143 (33.57%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 39  DDALLHALITVFGMRENGRIKKEKAKGVVEKLGLIQGEEEKA----RFDLPPGEGGDEVA 98
           D A L  +  +F    +G+I K++    +E LG+   +++      + DL    G   V 
Sbjct: 2   DQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDL---NGDGYVD 61

Query: 99  VEEVIGEMAEEAAAGKQSELLREAFKIFDVDGDGFIDTVELKRVLQCLGLDKGWGIREIE 158
           +EE  G          + E +REAF +FD + DGFI   EL+ VL  LGL +G  + + +
Sbjct: 62  IEEFGGLYQTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCK 121

Query: 159 KMVRVVDVNLDGKVDFGEFQLMM 178
           +M+  VDV+ DG V+F EF+ MM
Sbjct: 122 RMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of MS001235.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132261.1	3.8e-93	100.00	calmodulin-like protein 2 [Momordica charantia]	[more]
XP_022951438.1	3.6e-67	79.44	calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like p...	[more]
XP_023002517.1	1.0e-66	79.44	calmodulin-like protein 2 [Cucurbita maxima]	[more]
XP_038886166.1	1.0e-66	79.56	calmodulin-like protein 2 [Benincasa hispida]	[more]
KAG6585405.1	1.8e-66	78.89	putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of MS001235.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84UL5	1.8e-13	32.96	Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SU00	4.1e-13	33.57	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9FI19	6.9e-13	34.84	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q10LX4	9.1e-13	32.78	Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9ZR02	1.2e-12	33.57	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of MS001235.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BRS1	1.9e-93	100.00	calmodulin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111005158 PE=4 SV...	[more]
A0A6J1GHP6	1.7e-67	79.44	calmodulin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111454254 PE=4 SV=...	[more]
A0A6J1KJR1	5.1e-67	79.44	calmodulin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111496334 PE=4 SV=1	[more]
A0A5A7VBM3	1.3e-65	78.33	Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BBS0	1.3e-65	78.33	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1	[more]

BLAST of MS001235.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12860.1	2.9e-14	33.57	EF hand calcium-binding protein family	[more]
AT5G44460.1	4.9e-14	34.84	calmodulin like 43	[more]
AT4G03290.1	8.4e-14	33.57	EF hand calcium-binding protein family	[more]
AT4G20780.1	9.3e-13	48.10	calmodulin like 42	[more]
AT3G07490.1	1.2e-12	33.57	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 152..180 e-value: 0.24 score: 20.5 coord: 114..142 e-value: 1.9E-5 score: 34.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 114..177 e-value: 1.1E-10 score: 41.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 110..145 score: 15.657116
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 148..180 score: 11.528597
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 115..177 e-value: 1.45329E-16 score: 68.3433
None	No IPR available	GENE3D	1.10.238.10		coord: 17..179 e-value: 4.7E-21 score: 77.6
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 105..177
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 105..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 51..177

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MS001235	MS001235	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MS001235.1-cds	MS001235.1-cds-scaffold36:2548312..2548851	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MS001235.1	MS001235.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding