MS001220.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS001220.1 vs. NCBI nr
Match: XP_022131901.1 (ER membrane protein complex subunit 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1863.2 bits (4825), Expect = 0.0e+00
Identity = 945/949 (99.58%), Postives = 947/949 (99.79%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA
Sbjct: 21  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 80

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR
Sbjct: 81  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 140

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE
Sbjct: 141 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 200

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS
Sbjct: 201 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 260

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL
Sbjct: 261 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 320

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQ KGSYMHLSVKLID+WSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME
Sbjct: 321 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 380

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 381 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 440

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL
Sbjct: 441 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 500

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LLHPF KSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK
Sbjct: 501 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 560

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY
Sbjct: 561 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 620

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA
Sbjct: 621 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 680

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH
Sbjct: 681 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 740

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS
Sbjct: 741 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 800

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 801 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 860

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 861 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 920

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK
Sbjct: 921 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 969

BLAST of MS001220.1 vs. NCBI nr
Match: XP_022131900.1 (ER membrane protein complex subunit 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1863.2 bits (4825), Expect = 0.0e+00
Identity = 945/949 (99.58%), Postives = 947/949 (99.79%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA
Sbjct: 37  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR
Sbjct: 97  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS
Sbjct: 217 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL
Sbjct: 277 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQ KGSYMHLSVKLID+WSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME
Sbjct: 337 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL
Sbjct: 457 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LLHPF KSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK
Sbjct: 517 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY
Sbjct: 577 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA
Sbjct: 637 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH
Sbjct: 697 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 985

BLAST of MS001220.1 vs. NCBI nr
Match: KAG6585394.1 (ER membrane protein complex subunit 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1729.5 bits (4478), Expect = 0.0e+00
Identity = 856/949 (90.20%), Postives = 909/949 (95.79%), Query Frame = 0

Query: 1    RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
            RQQYLGK KHALFHS KSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF 
Sbjct: 71   RQQYLGKAKHALFHSSKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFV 130

Query: 61   LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
            LGKY++SLSSEGNFLRAWNLPDGQMVWESF+QGT PSKSLLLVPKSLK NQETVILVFG 
Sbjct: 131  LGKYVVSLSSEGNFLRAWNLPDGQMVWESFLQGTNPSKSLLLVPKSLKANQETVILVFGS 190

Query: 121  SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
            SCLHAVSSLDGEVIWKIDLTENSVEIQ+IIQHH+SD IYAVGFSSPTQFD+FQIN+KSGE
Sbjct: 191  SCLHAVSSLDGEVIWKIDLTENSVEIQKIIQHHESDTIYAVGFSSPTQFDRFQINVKSGE 250

Query: 181  LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
            L+KH T TF+GGFSGELVSVSDDVLVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE S
Sbjct: 251  LVKHHTATFSGGFSGELVSVSDDVLVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELS 310

Query: 241  GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
            GS+EIVPSKLSG+ A++V S L L+RVKGEGELEVVDKIH QATLSD+LLVSE Q A AL
Sbjct: 311  GSIEIVPSKLSGLFAVKVDSRLTLVRVKGEGELEVVDKIHSQATLSDALLVSEGQHAAAL 370

Query: 301  VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
            VQH+GS++ L+VKLIDNWSS+F++ENIVID  RG   KVFL+SYIRTDRSHGFRAL+VME
Sbjct: 371  VQHEGSHVRLTVKLIDNWSSNFIDENIVIDGHRGTFQKVFLHSYIRTDRSHGFRALLVME 430

Query: 361  DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
            DHSLLLVQQGE+VWSREDGLASI+NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKG
Sbjct: 431  DHSLLLVQQGEVVWSREDGLASIVNVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKG 490

Query: 421  TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
            TLMIASPEDVAAIQNMRLKSSDKSK+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS 
Sbjct: 491  TLMIASPEDVAAIQNMRLKSSDKSKMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSR 550

Query: 481  LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
            LLHP  KSEAC PRWLNIYQWQDPHHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+
Sbjct: 551  LLHPSHKSEACAPRWLNIYQWQDPHHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGR 610

Query: 541  EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
            EI SSSQ+HSV+QVIPLP TDSTEQRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWY
Sbjct: 611  EISSSSQIHSVLQVIPLPFTDSTEQRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWY 670

Query: 601  SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
            SVEADSGVIKGH+LKRNCI ++DDYCFESKDVWSI+LPSE+EKII+TA R LNEVVHTQA
Sbjct: 671  SVEADSGVIKGHSLKRNCIGVVDDYCFESKDVWSIILPSETEKIIATAARKLNEVVHTQA 730

Query: 661  KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
            KV ADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHH
Sbjct: 731  KVVADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHH 790

Query: 721  GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
            GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPIS
Sbjct: 791  GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPIS 850

Query: 781  SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
            SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 851  SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 910

Query: 841  NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
            NP+QAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 911  NPTQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFF 970

Query: 901  TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
            TRITPSRTYDSLTEDFSYALLL+TIVALV+AIF TW  SERKELQDKWK
Sbjct: 971  TRITPSRTYDSLTEDFSYALLLITIVALVIAIFATWVFSERKELQDKWK 1019

BLAST of MS001220.1 vs. NCBI nr
Match: XP_022951478.1 (ER membrane protein complex subunit 1 [Cucurbita moschata])

HSP 1 Score: 1729.1 bits (4477), Expect = 0.0e+00
Identity = 857/949 (90.31%), Postives = 908/949 (95.68%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGK KHALFHS KSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF 
Sbjct: 37  RQQYLGKAKHALFHSSKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFV 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKY++SLSSEGNFLRAWNLPDGQMVWE+F+QGT PSKSLLLVPKSLK N ETVILVFG 
Sbjct: 97  LGKYVVSLSSEGNFLRAWNLPDGQMVWETFLQGTNPSKSLLLVPKSLKANHETVILVFGS 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           L+KH T TF+GGFSGELVSVSDDVLVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE S
Sbjct: 217 LVKHHTATFSGGFSGELVSVSDDVLVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           GS+EIVPSKLSG+ A++V S L L+RVKGEGELEVVDKIH QATLSD+LLVSE Q A AL
Sbjct: 277 GSIEIVPSKLSGLFAVKVDSRLTLVRVKGEGELEVVDKIHSQATLSDALLVSEGQLAAAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQH+GS++HL+VKLIDNWSS+F++ENIVID  RG V KVFL+SYIRTDRSHGFRAL+VME
Sbjct: 337 VQHEGSHVHLTVKLIDNWSSNFIDENIVIDGHRGTVQKVFLHSYIRTDRSHGFRALLVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGE+VWSREDGLASI+NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEVVWSREDGLASIVNVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSK+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS 
Sbjct: 457 TLMIASPEDVAAIQNMRLKSSDKSKMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSR 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LL    KSEAC PRWLNIYQWQDPHHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+
Sbjct: 517 LLQASHKSEACAPRWLNIYQWQDPHHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGR 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EI SSSQ+HSV+QVIPLP TDSTEQRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWY
Sbjct: 577 EISSSSQIHSVLQVIPLPFTDSTEQRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGH+LKRNCID+ DDYCFESKDVWSI+LPSESEKII+TA R LNEVVHTQA
Sbjct: 637 SVEADSGVIKGHSLKRNCIDVADDYCFESKDVWSIILPSESEKIIATAARKLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KV ADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHH
Sbjct: 697 KVVADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NP+QAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPTQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLL+TIVALV+AIF TW  SERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLITIVALVIAIFATWVFSERKELQDKWK 985

BLAST of MS001220.1 vs. NCBI nr
Match: XP_023002203.1 (ER membrane protein complex subunit 1 [Cucurbita maxima])

HSP 1 Score: 1718.7 bits (4450), Expect = 0.0e+00
Identity = 852/949 (89.78%), Postives = 905/949 (95.36%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGK KHALFHS KSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF 
Sbjct: 37  RQQYLGKAKHALFHSSKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFV 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKY++SLSSEGNFLRAWNLPDGQMVWESF+QGT PSKSLLLVPKSLK NQETVILVFG 
Sbjct: 97  LGKYVVSLSSEGNFLRAWNLPDGQMVWESFLQGTNPSKSLLLVPKSLKANQETVILVFGS 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           L+KH T TF+GGFSGELVSVSDDVLVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE S
Sbjct: 217 LVKHHTATFSGGFSGELVSVSDDVLVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           G +EIV SKLSG+ A++V S L L+RVKGEGELEVVDKIH QATLSD+LLVSE Q A AL
Sbjct: 277 GLIEIVISKLSGLFAVKVDSRLTLVRVKGEGELEVVDKIHSQATLSDALLVSEGQHAAAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQH+GS++ L+VKLIDNWSS+F++ENIVID  RG V K FL+SYIR DRSHGFRAL+VME
Sbjct: 337 VQHEGSHVRLTVKLIDNWSSNFIDENIVIDGHRGTVQKFFLHSYIRKDRSHGFRALLVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGE+VWSREDGLASI+NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEVVWSREDGLASIVNVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSK+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS 
Sbjct: 457 TLMIASPEDVAAIQNMRLKSSDKSKMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSR 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LL P  KSEAC PRWLNIYQWQDPHHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+
Sbjct: 517 LLQPSHKSEACAPRWLNIYQWQDPHHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGR 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EI SSSQ+HSV+QVIPLP TDSTEQRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWY
Sbjct: 577 EISSSSQIHSVLQVIPLPFTDSTEQRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGH+LKRNCID++DDYCFESKDVWSI+LPSE+EKI +TA R LNEVVHTQA
Sbjct: 637 SVEADSGVIKGHSLKRNCIDVVDDYCFESKDVWSIILPSETEKITATAERKLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KV ADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHH
Sbjct: 697 KVVADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NP+QAE+EEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPTQAEKEEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLL+TIVALV+AIF TW  SERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLITIVALVIAIFATWVFSERKELQDKWK 985

BLAST of MS001220.1 vs. ExPASy Swiss-Prot
Match: Q5ZL00 (ER membrane protein complex subunit 1 OS=Gallus gallus OX=9031 GN=EMC1 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 9.4e-101
Identity = 283/1015 (27.88%), Postives = 489/1015 (48.18%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHV--LGPNDSIDGIE 60
           RQQY+GK+K A   + + G K+++V TE+NV+A+L+ R GEI WRH     P  +ID + 
Sbjct: 27  RQQYVGKLKFASLEAAQ-GSKKLLVGTEKNVVAALNSRSGEILWRHADKATPEGAIDAM- 86

Query: 61  FALGKYIISLSSEGNFLRAWNLPDGQMVWESFIQ-GTKPSKSLLLVPKSLKPNQETVILV 120
              G+  I++SS G  LR+W    G + WE+ +  G+  + SL+ +  ++K      +L 
Sbjct: 87  LIHGQDAITVSSAGRILRSWETNIGGLNWETSLDTGSFQTASLVGLQDAVK---YVAVLK 146

Query: 121 FGRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIK 180
                LH +S  +G   W   L E+     Q++    + +I+ +G    +      ++++
Sbjct: 147 KAAISLHYLS--NGHQKWVEHLPESENTQYQMLYSRGAGVIHVLGVVPQSHLKVLTLSVE 206

Query: 181 SGELIKHS--TETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHL 240
            GE+I+ +     +    +G    V + VLV  D    +L + SL + E  ++Q P+  L
Sbjct: 207 DGEVIEQTKVAAPWLKSLNGACGVVGEAVLVCADTATHSLYVCSL-ETEQEMKQIPLQSL 266

Query: 241 VDEFSGSVE----------IVPSKLSGILAIEVG--------------------SVLILI 300
             EF+   +          I  S+    L +  G                    + L+  
Sbjct: 267 DLEFADGFQPRVLATQPSVINASRTQFFLQLSPGHFSLLQCKQGLLSHLRDFQQAALVSF 326

Query: 301 RVKGEGELEVV------------DKIHGQATLSDSLLVSESQRAVALVQHKGSY------ 360
              GE  +  V            D +HG A       + +SQ+  AL     +Y      
Sbjct: 327 ATTGEKTVAAVLTCRNELKPASSDGLHGNA-------LEDSQKQEALTCSNQTYNINLYL 386

Query: 361 MHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLV 420
           +    +L+D   +  LE+N    +Q      +++  +++ D S G+RAL+  EDH L+ +
Sbjct: 387 VETGQRLLDTTITFNLEQNGAKPEQ------LYIQVFLKKDDSVGYRALVQTEDHMLMFL 446

Query: 421 QQ-GEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIAS 480
           QQ G++VWSRE+ LA ++++   +LP+      +        + L G  LK   + +I  
Sbjct: 447 QQPGKVVWSREESLAEVVSLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILL 506

Query: 481 PEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLTKSGKLYALHSGDGRI 540
               A +  M     + +S  K++     L+RD    +K+++++T SGKL+ + S  G I
Sbjct: 507 QAWTAHLWKMFYDARKPRSQIKNEINIDNLARDEFNLQKMMVMVTASGKLFGIESSSGTI 566

Query: 541 VWSLLLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDT 600
           +W   L       +     L +   +   H       ++L+  +  +      FL   + 
Sbjct: 567 LWKQYLRNVRPGAS-----LKLMVQRTTAHFPHPPQCTLLVKDKETKM----SFLYVFNP 626

Query: 601 YTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEF 660
             GK  ++        V+Q + LP+ D    ++ +LID E +   +P T   +  L+   
Sbjct: 627 IFGKRSQVAPPVLKRPVLQTLLLPIMDQDYAKVLLLIDDEYKVTAFPATKNVLRQLEEMA 686

Query: 661 SNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNE 720
            +I++Y V+A+ G + G  LK++           +++ W + +P+E ++I++   +  +E
Sbjct: 687 HSIFFYLVDAEQGKLSGFRLKKD---------LTTEESWQVAIPTEVQRIVTVKGKRSSE 746

Query: 721 VVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRIL 780
            VH+Q +V  D+ V+YK ++ NLL + T       E   T  E +++ +YLID V GRI+
Sbjct: 747 HVHSQGRVMGDRSVLYKSLNPNLLAVVT-------ESTDTHHERTFVGIYLIDGVTGRII 806

Query: 781 HRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHN 840
           H      + GPVH V SENWVVY Y+N KA R E +V+E+Y+ +   N   +       +
Sbjct: 807 HSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFS------S 866

Query: 841 LTSPISSYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYL 900
           L  P+     P++L   QSY F  ++ A+  T T +GITS+ LLIG  +  IL+L K  L
Sbjct: 867 LDRPL----LPQVL--QQSYIFPSAISAMEATITERGITSRHLLIGLPSGAILSLPKALL 926

Query: 901 DPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAY 950
           DPRR   P++  REE +IP +  + I  + +  +   V  +RG+ T P+ LEST L  AY
Sbjct: 927 DPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSQIRGIYTSPSGLESTCLVVAY 983

BLAST of MS001220.1 vs. ExPASy Swiss-Prot
Match: Q8N766 (ER membrane protein complex subunit 1 OS=Homo sapiens OX=9606 GN=EMC1 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.0e-94
Identity = 281/1008 (27.88%), Postives = 494/1008 (49.01%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHV-LGPNDSIDGIEF 60
           RQQY+GKVK A       G K++VV+TE+NVIA+L+ R GEI WRHV  G  +       
Sbjct: 33  RQQYVGKVKFASLEF-SPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAML 92

Query: 61  ALGKYIISLSSEGNFLRAWNLPDGQMVWE-SFIQGTKPSKSLLLVPKSLKPNQETVILVF 120
             G+ +I++S+ G  +R+W    G + WE +   G+  +  L+ + +S++      +L  
Sbjct: 93  LHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVR---YIAVLKK 152

Query: 121 GRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKS 180
               LH +SS  G + W   L E+     Q++  + S +++A+G    +  +  + N++ 
Sbjct: 153 TTLALHHLSS--GHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVED 212

Query: 181 GELIK--HSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLV 240
           GE+++    +  +    SG    V + VLV  D +  +L  L+L + E  ++Q P+  L 
Sbjct: 213 GEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLAL-ETEWELRQIPLQSLD 272

Query: 241 DEFSGSVE--IVPSKLS------------------GILAIEVGSV----------LILIR 300
            EF    +  ++P++ +                   +L    G++          L+   
Sbjct: 273 LEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFA 332

Query: 301 VKGEGELEVV----DKIHGQATLSDSLLVSESQRAV---ALVQHKGSYMHLSVKLIDNWS 360
             GE  +  V    +++   ++  D  + S S+++    +L     +Y  +++ L++   
Sbjct: 333 TTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYT-INLYLVET-G 392

Query: 361 SDFLEENIVID-DQRGF-VHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQ--GEIV-W 420
              L+  I    +Q G    ++++  +++ D S G+RAL+  EDH LL +QQ  G++V W
Sbjct: 393 RRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLW 452

Query: 421 SREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAAIQ 480
           SRE+ LA ++ +   +LP+      +        + L G  LK   + +I      + + 
Sbjct: 453 SREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLW 512

Query: 481 NM-----RLKSSDKSK-----LSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLLHP 540
            M     + +S  K++     L+RD    +K+++++T SGKL+ + S  G I+W   L P
Sbjct: 513 KMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYL-P 572

Query: 541 FCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK--EI 600
             K ++     +       PH       P   ++ +  +S  G   L   +   GK  ++
Sbjct: 573 NVKPDSSFKLMVQRTTAHFPH------PPQCTLLVKDKES--GMSSLYVFNPIFGKWSQV 632

Query: 601 GSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWYSV 660
                   ++Q + LP+ D    ++ +LID E +   +P T   +  L     +I++Y V
Sbjct: 633 APPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLV 692

Query: 661 EADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQAKV 720
           +A+ G + G+ L+++    L          W + +P E ++I+    +  +E VH+Q +V
Sbjct: 693 DAEQGRLCGYRLRKDLTTELS---------WELTIPPEVQRIVKVKGKRSSEHVHSQGRV 752

Query: 721 AADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHHGS 780
             D+ V+YK ++ NLL + T       E      E +++ ++LID V GRI+H      +
Sbjct: 753 MGDRSVLYKSLNPNLLAVVT-------ESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKA 812

Query: 781 TGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPISSY 840
            GPVH V SENWVVY Y+N KA R E +V+E+Y+ +             ++N T+  SS 
Sbjct: 813 KGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTE------------QYNATA-FSSL 872

Query: 841 SRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSIN 900
            RP++     QSY F  S+ A+  T T +GITS+ LLIG  +  IL+L K  LDPRR   
Sbjct: 873 DRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEI 932

Query: 901 PSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFFT 950
           P++  REE +IP +  + I  + +  +   V  +RG+ T P+ LEST L  AYG+D++ T
Sbjct: 933 PTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQT 992

BLAST of MS001220.1 vs. ExPASy Swiss-Prot
Match: Q5R7K6 (ER membrane protein complex subunit 1 OS=Pongo abelii OX=9601 GN=EMC1 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.5e-93
Identity = 281/1019 (27.58%), Postives = 497/1019 (48.77%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHV-LGPNDSIDGIEF 60
           RQQY+GK+K A       G K++VV+TE+NVIA+L+ R GEI WRHV  G  +       
Sbjct: 33  RQQYVGKLKFASLEF-SPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAML 92

Query: 61  ALGKYIISLSSEGNFLRAWNLPDGQMVWESFIQ-GTKPSKSLLLVPKSLKPNQETVILVF 120
             G+ +I++S+ G  +R+W    G + WE  +  G+  +  L+ + +S++      +L  
Sbjct: 93  LHGQDVITVSNGGRIMRSWETNIGGLNWEITLDTGSFQALGLVGLQESVR---YIAVLKK 152

Query: 121 GRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKS 180
               LH +SS  G + W   L E+     Q++  + S +++A+G    +  +  + N++ 
Sbjct: 153 TTLALHHLSS--GHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVED 212

Query: 181 GELIK--HSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLV 240
           GE+++    +  +    SG    V + VLV  D +  +L  L+L + E  ++Q P+  L 
Sbjct: 213 GEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLAL-ETEWELRQIPLQSLD 272

Query: 241 DEFSGSVE--IVPSKLS-------------------------GILAIEVG---SVLILIR 300
            EF    +  ++P++ +                         GIL++      + L+   
Sbjct: 273 LEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGILSLLKNFPQTALVSFA 332

Query: 301 VKGEGELEVV----DKIHGQATLSDSLLVSESQRAV---ALVQHKGSY------MHLSVK 360
             GE  +  V    +++   +   D  + S S+++    +L     +Y      +    +
Sbjct: 333 TTGEKTVAAVMACRNEVQKTSNSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRR 392

Query: 361 LIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQ--GE 420
           L+D  ++  LE++    +      ++++  +++ D S G+RAL+  EDH LL +QQ  G+
Sbjct: 393 LLDTTTTFSLEQSGTRPE------RLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGK 452

Query: 421 IV-WSREDGLASIINVVTSELPVEKKGVSI-------AKVENNLIEWLQGHLLKLKGTLM 480
           +V WSRE+ LA ++ +   +LP+      +       A +++ L+    G  LK   + +
Sbjct: 453 VVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLL----GMFLKRLSSQL 512

Query: 481 IASPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLTKSGKLYALHSGD 540
           I      + +  M     + +S  K++     L+RD    +K+++++T SGKL+ + S  
Sbjct: 513 ILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSS 572

Query: 541 GRIVWSLLLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSF 600
           G I+W   L P  K ++     +       PH       P   ++ +  +S  G   L  
Sbjct: 573 GTILWKQYL-PSVKPDSSFKLMVQRTTAHFPH------PPQCTLLVKDKES--GMSSLYV 632

Query: 601 VDTYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQ 660
            +   GK  ++        ++Q + LP+ D    ++ +LID E +   +P T   +  L 
Sbjct: 633 FNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLH 692

Query: 661 SEFSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRN 720
               +I++Y V+A+ G + G+ L+++    L          W + +P E  +I+    + 
Sbjct: 693 ELAPSIFFYLVDAEQGRLCGYRLRKDLTTELS---------WELTIPPEVRRIVKVKGKR 752

Query: 721 LNEVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNG 780
            +E VH+Q +V  D+ V+YK ++ NLL + T       E      E +++ ++LID V G
Sbjct: 753 SSEHVHSQGRVMGDRSVLYKSLNPNLLAVVT-------ESTDAHHERTFIGIFLIDGVTG 812

Query: 781 RILHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILG 840
           RI+H      + GPVH V SENWVVY Y+N KA R E +V+E+Y+ +             
Sbjct: 813 RIIHSSAQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTE------------ 872

Query: 841 KHNLTSPISSYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALD 900
           ++N T+  SS  RP++     QSY F  S+ A+  T T +GITS+ LLIG  +  IL+L 
Sbjct: 873 QYNATA-FSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLP 932

Query: 901 KRYLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTL 950
           K  LDPRR   P++  REE +IP +  + I  + +  +   V  +RG+ T P+ LEST L
Sbjct: 933 KALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCL 992

BLAST of MS001220.1 vs. ExPASy Swiss-Prot
Match: Q6NRB9 (ER membrane protein complex subunit 1 OS=Xenopus laevis OX=8355 GN=emc1 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 5.5e-93
Identity = 269/1010 (26.63%), Postives = 466/1010 (46.14%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQ+Y+G++K A   S   G K+++  T++N+IA+L+ R G++ WRHV    D+ +G   A
Sbjct: 32  RQEYVGRIKFASLES-GLGAKKLIAVTDKNIIAALNSRTGDLLWRHV--DKDTSEGTVDA 91

Query: 61  L---GKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILV 120
           L   G+  I++S  G  LR+W    G + WE+ ++      S   V  +   +    + V
Sbjct: 92  LMMIGQDAITVSG-GRLLRSWETNIGALNWEAALE----PGSFQAVSFAGSQDTARYVAV 151

Query: 121 FGRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIK 180
              S L      +G + W   L E+     Q++       ++ VG    +       +++
Sbjct: 152 LKNSALSLYFLSNGHLKWSESLPESDTVQYQLLYSPYKGSVHVVGLVPHSHLTILTFSLE 211

Query: 181 SGELIKHSTETFT---GGFSGELVSVSDDVLVTLDATRSNLVILSLRDGE---------- 240
            G  I H     T       G    + + VLV  D   +++ I+SL  GE          
Sbjct: 212 DGS-ISHQVRVLTPWLRTLHGTCGVIGEGVLVCGDVPMASVHIVSLLSGEETTRYSVQSL 271

Query: 241 -------------IGIQQSPIAHLVDEFSGSVEIVPSKL------SGILA-IEVGSVLIL 300
                        I   Q+ I   + +F   ++I P +        G+L  +   S + L
Sbjct: 272 DIELAEDPTQLDVITAPQNGIGGSLSQF--FLQIAPRRFLLMHYHDGVLTPLRDFSQVSL 331

Query: 301 IRVKGEGELEVVD----KIHGQAT-------LSDSLLVSESQRAVALVQHKGSYMHLSVK 360
           +     GE  VV     K  G          L+      E             YM  S +
Sbjct: 332 VNFATTGEKTVVAVMQCKTEGNPKSGAESEYLTGQNCAQEPWYCPGHTYSINLYMADSGR 391

Query: 361 LIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQ-GEI 420
            +   +  F  + I +          +L +++R D S G+RAL+  ED+ LL +QQ G++
Sbjct: 392 RLLETTMSFTLDQICVRPD-----SFYLQTFLRKDDSVGYRALVQTEDNQLLFLQQPGKL 451

Query: 421 VWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGTLMIASPEDVAA 480
           +W RE+ LA ++ + T +LP+      +        + L G +LK   + +I      A 
Sbjct: 452 IWLREESLADVVTMETVDLPLTGAQAELEGEFGKKADGLIGMVLKRLSSQLILLQSWSAH 511

Query: 481 IQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLLL 540
           +  M     + +S  +++     L+RD    +K+++++T SGKL+ + S  G I+W   L
Sbjct: 512 LWKMFCDARKPRSQIRNEINVDTLARDDFNLQKMMVMVTASGKLFGIESSSGSILWKFYL 571

Query: 541 HPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK-- 600
           H      +       +   +   H       ++L+  +  +       +   +   GK  
Sbjct: 572 HGVHPGSS-----FKLLVQRTTAHFPHPPQCTLLVKDKVTEKSA----MYVFNPIFGKLS 631

Query: 601 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 660
           ++        ++Q + LP+ D+   ++ +L+D + +   +P T   +  LQ   S I++Y
Sbjct: 632 QLAPPPLQRPILQSLLLPIMDNDYAKVLLLLDDQHKVIAFPATKYVLQQLQELHSTIFFY 691

Query: 661 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 720
            V+ + G + G  L ++           ++++W ++LP++ ++I     +  NE VH+Q 
Sbjct: 692 LVDVEKGKLSGLRLNKD---------LSTEEIWEVLLPADQQRITVVKGKRSNEHVHSQG 751

Query: 721 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 780
           +V  D+ V+YKY++ NLL L T       E   T PE  ++ +YLID V GRI+H     
Sbjct: 752 RVMGDRSVLYKYLNPNLLVLVT-------ESTDTHPERCFIGIYLIDGVTGRIIHSSVQR 811

Query: 781 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 840
            + GPV  + SENWVVY Y+N KA R E++V+E+Y+ +   N             ++  S
Sbjct: 812 RARGPVQIIHSENWVVYQYWNSKARRNELTVLELYEGTEQYN-------------STNFS 871

Query: 841 SYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRS 900
           S  RP +     QSY F  +++A+  T T +GITS+ +LIG  +  IL+L K  LDPRR 
Sbjct: 872 SLDRPLLPHVLQQSYIFPSAIRAMQATITERGITSRHILIGLPSGAILSLPKALLDPRRP 931

Query: 901 INPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLF 950
             P++  REE +IP T  + I  + +  +   +  +RG+ T P+ LEST L  AYG+DL+
Sbjct: 932 EIPNEYTREENLIPYTPDIQIHAERFINYNQTISRMRGIYTAPSGLESTCLVVAYGLDLY 987

BLAST of MS001220.1 vs. ExPASy Swiss-Prot
Match: Q8C7X2 (ER membrane protein complex subunit 1 OS=Mus musculus OX=10090 GN=Emc1 PE=1 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 4.0e-91
Identity = 276/1017 (27.14%), Postives = 486/1017 (47.79%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHV-LGPNDSIDGIEF 60
           RQQY+GK+K A       G K++VV+TE+NVIA+L+ R GEI WRHV  G  +       
Sbjct: 34  RQQYVGKIKFASLEF-SPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAML 93

Query: 61  ALGKYIISLSSEGNFLRAWNLPDGQMVWESFIQ-GTKPSKSLLLVPKSLKPNQETVILVF 120
             G+  I++S+ G  +R+W    G + WE  +  G+  +  L+ + +S++      +L  
Sbjct: 94  VHGQDAITVSNGGRLMRSWETNIGGLNWEITLDTGSFQALGLVGLQESVR---YIAVLKK 153

Query: 121 GRSCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKS 180
               LH +SS  G + W   L E+   + Q++  + S +++A+G    +  +  + N++ 
Sbjct: 154 TTLTLHHLSS--GHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVED 213

Query: 181 GELIKHST--ETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLV 240
           GE+++       +    +G    V + VLV  D +  +L  L+L + E  ++Q P+    
Sbjct: 214 GEIVQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLAL-ETEWELRQIPLQSPD 273

Query: 241 DEFSGSV--EIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATL----SDSLL 300
            EF      +++P++ S +        L L          ++   HG  TL      + L
Sbjct: 274 LEFGSGFQPQVLPTQPSPVAPSRAQFFLQL----SPSHYALLHYHHGAVTLLKNFPQATL 333

Query: 301 VS---ESQRAVALVQHKGSYMHLSVKLIDNWSSDFLEENIVID----------------- 360
           VS     ++ VA V    + +   V   D   + F E +   D                 
Sbjct: 334 VSFATTGEKTVAAVMTCRTEVQKPVSAGDGSVASFPETSGAQDSLACFNQTYTINLYLVE 393

Query: 361 --------------DQRGF-VHKVFLNSYIRTDRSHGFRALIVMEDHSLLLVQQ--GEIV 420
                         +Q+G    ++++  +++ D S G+RAL+  +DH  L +QQ  G++V
Sbjct: 394 TGRRLLDTSISFSLEQKGTRPEQLYIQVFLKKDDSVGYRALVQTQDHLQLFLQQLAGKVV 453

Query: 421 -WSREDGLASIINVVTSELPVEKKGVSI-------AKVENNLIEWLQGHLLKLKGTLMIA 480
            WSRE+ LA ++ +   +LP+      +       A +++ L+    G  LK   + +I 
Sbjct: 454 LWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLL----GMFLKRLSSQLIL 513

Query: 481 SPEDVAAIQNM-----RLKSSDKSK-----LSRDHNGFRKLLIVLTKSGKLYALHSGDGR 540
                + +  M     + +S  K++     L+RD    +K+++ +T SGKL+ + S  G 
Sbjct: 514 LQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVTVTASGKLFGIESSSGT 573

Query: 541 IVWSLLLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVD 600
           I+W   L P  K ++     +       PH       P   ++ +  ++  G   L   +
Sbjct: 574 ILWKQYL-PNVKPDSSFKLMVQRTTAHFPH------PPQCTLLVKDKET--GMSSLFVFN 633

Query: 601 TYTGK--EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSE 660
              GK  ++        ++Q + LP+ D    ++ +L+D E +   +P T   +  L   
Sbjct: 634 PIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHEL 693

Query: 661 FSNIYWYSVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLN 720
             +I++Y V+A+ G + G+ L+++    L          W + +P E ++++    +  +
Sbjct: 694 APSIFFYLVDAEQGRLSGYQLRKDLTTELS---------WELTIPPEVQRVVKVKGKRSS 753

Query: 721 EVVHTQAKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRI 780
           E VH+Q +V  D+ V+YK ++ NLL + T       E      E +++ ++LID V GRI
Sbjct: 754 EHVHSQGRVMGDRSVLYKSLNPNLLAVVT-------ESTDVHHERTFIGIFLIDGVTGRI 813

Query: 781 LHRMTHHGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKH 840
           +H      + GPVH V SENWVVY Y+N KA R E++ +E+Y+ +             ++
Sbjct: 814 IHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTE------------QY 873

Query: 841 NLTSPISSYSRPEI-LAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKR 900
           N T+  SS  RP++     QSY F  S+ A+  T T +GITS+ LLIG  +  IL+L K 
Sbjct: 874 NATA-FSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKA 933

Query: 901 YLDPRRSINPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAF 950
            LDPRR   P++  REE +IP +  + +  + +  +   V  +RG+ T P+ LEST L  
Sbjct: 934 LLDPRRPEIPTEQSREENLIPYSPDVQVHAERFINYNQTVSRMRGIYTAPSGLESTCLVV 993

BLAST of MS001220.1 vs. ExPASy TrEMBL
Match: A0A6J1BQR7 (ER membrane protein complex subunit 1 OS=Momordica charantia OX=3673 GN=LOC111004927 PE=3 SV=1)

HSP 1 Score: 1863.2 bits (4825), Expect = 0.0e+00
Identity = 945/949 (99.58%), Postives = 947/949 (99.79%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA
Sbjct: 21  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 80

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR
Sbjct: 81  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 140

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE
Sbjct: 141 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 200

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS
Sbjct: 201 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 260

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL
Sbjct: 261 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 320

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQ KGSYMHLSVKLID+WSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME
Sbjct: 321 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 380

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 381 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 440

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL
Sbjct: 441 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 500

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LLHPF KSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK
Sbjct: 501 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 560

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY
Sbjct: 561 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 620

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA
Sbjct: 621 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 680

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH
Sbjct: 681 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 740

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS
Sbjct: 741 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 800

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 801 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 860

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 861 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 920

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK
Sbjct: 921 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 969

BLAST of MS001220.1 vs. ExPASy TrEMBL
Match: A0A6J1BQZ1 (ER membrane protein complex subunit 1 OS=Momordica charantia OX=3673 GN=LOC111004927 PE=3 SV=1)

HSP 1 Score: 1863.2 bits (4825), Expect = 0.0e+00
Identity = 945/949 (99.58%), Postives = 947/949 (99.79%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA
Sbjct: 37  RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR
Sbjct: 97  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS
Sbjct: 217 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL
Sbjct: 277 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQ KGSYMHLSVKLID+WSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME
Sbjct: 337 VQRKGSYMHLSVKLIDSWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL
Sbjct: 457 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LLHPF KSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK
Sbjct: 517 LLHPFRKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY
Sbjct: 577 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA
Sbjct: 637 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH
Sbjct: 697 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTLPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 985

BLAST of MS001220.1 vs. ExPASy TrEMBL
Match: A0A6J1GHT1 (ER membrane protein complex subunit 1 OS=Cucurbita moschata OX=3662 GN=LOC111454284 PE=3 SV=1)

HSP 1 Score: 1729.1 bits (4477), Expect = 0.0e+00
Identity = 857/949 (90.31%), Postives = 908/949 (95.68%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGK KHALFHS KSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF 
Sbjct: 37  RQQYLGKAKHALFHSSKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFV 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKY++SLSSEGNFLRAWNLPDGQMVWE+F+QGT PSKSLLLVPKSLK N ETVILVFG 
Sbjct: 97  LGKYVVSLSSEGNFLRAWNLPDGQMVWETFLQGTNPSKSLLLVPKSLKANHETVILVFGS 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           L+KH T TF+GGFSGELVSVSDDVLVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE S
Sbjct: 217 LVKHHTATFSGGFSGELVSVSDDVLVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           GS+EIVPSKLSG+ A++V S L L+RVKGEGELEVVDKIH QATLSD+LLVSE Q A AL
Sbjct: 277 GSIEIVPSKLSGLFAVKVDSRLTLVRVKGEGELEVVDKIHSQATLSDALLVSEGQLAAAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQH+GS++HL+VKLIDNWSS+F++ENIVID  RG V KVFL+SYIRTDRSHGFRAL+VME
Sbjct: 337 VQHEGSHVHLTVKLIDNWSSNFIDENIVIDGHRGTVQKVFLHSYIRTDRSHGFRALLVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGE+VWSREDGLASI+NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEVVWSREDGLASIVNVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSK+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS 
Sbjct: 457 TLMIASPEDVAAIQNMRLKSSDKSKMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSR 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LL    KSEAC PRWLNIYQWQDPHHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+
Sbjct: 517 LLQASHKSEACAPRWLNIYQWQDPHHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGR 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EI SSSQ+HSV+QVIPLP TDSTEQRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWY
Sbjct: 577 EISSSSQIHSVLQVIPLPFTDSTEQRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGH+LKRNCID+ DDYCFESKDVWSI+LPSESEKII+TA R LNEVVHTQA
Sbjct: 637 SVEADSGVIKGHSLKRNCIDVADDYCFESKDVWSIILPSESEKIIATAARKLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KV ADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHH
Sbjct: 697 KVVADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NP+QAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPTQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLL+TIVALV+AIF TW  SERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLITIVALVIAIFATWVFSERKELQDKWK 985

BLAST of MS001220.1 vs. ExPASy TrEMBL
Match: A0A6J1KSW0 (ER membrane protein complex subunit 1 OS=Cucurbita maxima OX=3661 GN=LOC111496131 PE=3 SV=1)

HSP 1 Score: 1718.7 bits (4450), Expect = 0.0e+00
Identity = 852/949 (89.78%), Postives = 905/949 (95.36%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGK KHALFHS KSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEF 
Sbjct: 37  RQQYLGKAKHALFHSSKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFV 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKY++SLSSEGNFLRAWNLPDGQMVWESF+QGT PSKSLLLVPKSLK NQETVILVFG 
Sbjct: 97  LGKYVVSLSSEGNFLRAWNLPDGQMVWESFLQGTNPSKSLLLVPKSLKANQETVILVFGS 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQ+IIQHH+SD IYAVGFSSPTQFDQFQIN+KSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQKIIQHHESDTIYAVGFSSPTQFDQFQINVKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           L+KH T TF+GGFSGELVSVSDDVLVTLDAT+SNLVI++LR+GEIGI Q+PIAHL+DE S
Sbjct: 217 LVKHHTATFSGGFSGELVSVSDDVLVTLDATKSNLVIINLRNGEIGILQTPIAHLIDELS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           G +EIV SKLSG+ A++V S L L+RVKGEGELEVVDKIH QATLSD+LLVSE Q A AL
Sbjct: 277 GLIEIVISKLSGLFAVKVDSRLTLVRVKGEGELEVVDKIHSQATLSDALLVSEGQHAAAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
           VQH+GS++ L+VKLIDNWSS+F++ENIVID  RG V K FL+SYIR DRSHGFRAL+VME
Sbjct: 337 VQHEGSHVRLTVKLIDNWSSNFIDENIVIDGHRGTVQKFFLHSYIRKDRSHGFRALLVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGE+VWSREDGLASI+NVVTSELPVEKKGVSIAKVE+NLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEVVWSREDGLASIVNVVTSELPVEKKGVSIAKVEHNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDVAAIQNMRLKSSDKSK+SRDHNGFRKLLIVLTKSGKL+ALHSGDGRIVWS 
Sbjct: 457 TLMIASPEDVAAIQNMRLKSSDKSKMSRDHNGFRKLLIVLTKSGKLFALHSGDGRIVWSR 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LL P  KSEAC PRWLNIYQWQDPHHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTG+
Sbjct: 517 LLQPSHKSEACAPRWLNIYQWQDPHHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGR 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EI SSSQ+HSV+QVIPLP TDSTEQRLHILIDA+SRAHLYPQTSEAISILQSEFSN+YWY
Sbjct: 577 EISSSSQIHSVLQVIPLPFTDSTEQRLHILIDAQSRAHLYPQTSEAISILQSEFSNVYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSGVIKGH+LKRNCID++DDYCFESKDVWSI+LPSE+EKI +TA R LNEVVHTQA
Sbjct: 637 SVEADSGVIKGHSLKRNCIDVVDDYCFESKDVWSIILPSETEKITATAERKLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KV ADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVY+IDIVNGRILHRMTHH
Sbjct: 697 KVVADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYVIDIVNGRILHRMTHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLI+GKHNLTSPIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTSPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NP+QAE+EEGVIPLTDSLPIIPQTYATHALQVEGLRGVVT+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPTQAEKEEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTIPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLL+TIVALV+AIF TW  SERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLITIVALVIAIFATWVFSERKELQDKWK 985

BLAST of MS001220.1 vs. ExPASy TrEMBL
Match: A0A1S3BCL2 (ER membrane protein complex subunit 1 OS=Cucumis melo OX=3656 GN=LOC103488192 PE=3 SV=1)

HSP 1 Score: 1708.3 bits (4423), Expect = 0.0e+00
Identity = 845/949 (89.04%), Postives = 898/949 (94.63%), Query Frame = 0

Query: 1   RQQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFA 60
           RQQYLGK KHALFHS KSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPND IDGIEF 
Sbjct: 37  RQQYLGKAKHALFHSSKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDPIDGIEFV 96

Query: 61  LGKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGR 120
           LGKY++SLSSEGNFLRAWNLPDGQM WESF+QGT PSKSLLLVPKSLK NQETVILVF R
Sbjct: 97  LGKYVVSLSSEGNFLRAWNLPDGQMAWESFLQGTSPSKSLLLVPKSLKANQETVILVFSR 156

Query: 121 SCLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGE 180
           SCLHAVSSLDGEVIWKIDLTENSVEIQ+IIQ HDSDIIYAVGFSSPTQ DQFQIN+KSGE
Sbjct: 157 SCLHAVSSLDGEVIWKIDLTENSVEIQKIIQLHDSDIIYAVGFSSPTQLDQFQINVKSGE 216

Query: 181 LIKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFS 240
           L+KH T TF+GGFSGELVSVSDDVLVT+D  RSNLVI++ ++GEIGI QSPIA ++DEFS
Sbjct: 217 LLKHQTATFSGGFSGELVSVSDDVLVTVDTARSNLVIINFKNGEIGILQSPIAPVIDEFS 276

Query: 241 GSVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVAL 300
           G +EIVPSKLSG+LA++V S+L L+RVKGEGELEVVDKI GQAT+SD+LLVSE+Q A AL
Sbjct: 277 GLMEIVPSKLSGLLAVKVNSLLTLVRVKGEGELEVVDKIPGQATVSDALLVSENQHAAAL 336

Query: 301 VQHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVME 360
             H+GS+MHL+VKLIDNWS+ F++ENIVID QRG V KVFLNSYIRTDRSHGFRAL+VME
Sbjct: 337 AHHEGSHMHLTVKLIDNWSTSFIDENIVIDKQRGSVQKVFLNSYIRTDRSHGFRALLVME 396

Query: 361 DHSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 420
           DHSLLLVQQGEIVWSREDGLASI+NVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG
Sbjct: 397 DHSLLLVQQGEIVWSREDGLASIVNVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKG 456

Query: 421 TLMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSL 480
           TLMIASPEDV AIQNMRLKSSDKSK+SRDHNGFRKLLIVLTKSGKL+ALHSGDGR+VWS 
Sbjct: 457 TLMIASPEDVVAIQNMRLKSSDKSKMSRDHNGFRKLLIVLTKSGKLFALHSGDGRVVWSR 516

Query: 481 LLHPFCKSEACTPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 540
           LL PF KS+ C PRWLNIYQWQDPHHRAMDENPSVL+VGRCGQSM GPG LSFVDTYTGK
Sbjct: 517 LLQPFHKSKDCAPRWLNIYQWQDPHHRAMDENPSVLVVGRCGQSMDGPGLLSFVDTYTGK 576

Query: 541 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 600
           EI SSSQ HS+V+VIPLP TDSTE+RLHILIDAE RAHLYPQTSEAI ILQSEFSNIYWY
Sbjct: 577 EISSSSQTHSIVKVIPLPFTDSTERRLHILIDAEGRAHLYPQTSEAIGILQSEFSNIYWY 636

Query: 601 SVEADSGVIKGHALKRNCIDILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQA 660
           SVEADSG+IKGHAL R C+D++DDYCFESKDVW IMLPS+SEKII++ATR LNEVVHTQA
Sbjct: 637 SVEADSGIIKGHALMRKCVDVVDDYCFESKDVWLIMLPSDSEKIIASATRKLNEVVHTQA 696

Query: 661 KVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTHH 720
           KV ADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRM HH
Sbjct: 697 KVVADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMIHH 756

Query: 721 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPIS 780
           GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLI+GKHNLT+PIS
Sbjct: 757 GSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLIIGKHNLTTPIS 816

Query: 781 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 840
           SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI
Sbjct: 817 SYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRSI 876

Query: 841 NPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLFF 900
           NPSQAEREEGVIPLTDSLPIIPQTY THALQVEGLRG++T+PAKLESTTLAFAYGVDLFF
Sbjct: 877 NPSQAEREEGVIPLTDSLPIIPQTYVTHALQVEGLRGIMTIPAKLESTTLAFAYGVDLFF 936

Query: 901 TRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           TRITPSRTYDSLTEDFSYALLL+TIVALV+AIF TW LSERKELQDKWK
Sbjct: 937 TRITPSRTYDSLTEDFSYALLLITIVALVIAIFATWVLSERKELQDKWK 985

BLAST of MS001220.1 vs. TAIR 10
Match: AT5G11560.1 (catalytics )

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 614/950 (64.63%), Postives = 781/950 (82.21%), Query Frame = 0

Query: 2   QQYLGKVKHALFHSQKSGRKRVVVSTEENVIASLDLRHGEIFWRHVLGPNDSIDGIEFAL 61
           Q+Y+GKVKHA+FH+QK+GRKRV+VSTEENV+ASLDLRHGEIFWRHVLG  D+IDG+  AL
Sbjct: 34  QRYIGKVKHAVFHTQKTGRKRVIVSTEENVVASLDLRHGEIFWRHVLGTKDAIDGVGIAL 93

Query: 62  GKYIISLSSEGNFLRAWNLPDGQMVWESFIQGTKPSKSLLLVPKSLKPNQETVILVFGRS 121
           GKY+I+LSSEG+ LRAWNLPDGQMVWE+ +   + SKSLL VP +LK +++  I VFG  
Sbjct: 94  GKYVITLSSEGSTLRAWNLPDGQMVWETSLHTAQHSKSLLSVPINLKVDKDYPITVFGGG 153

Query: 122 CLHAVSSLDGEVIWKIDLTENSVEIQQIIQHHDSDIIYAVGFSSPTQFDQFQINIKSGEL 181
            LHAVS++DGEV+WK D T    E+Q+++Q   S IIY +GF   ++   +QI+ KSGE+
Sbjct: 154 YLHAVSAIDGEVLWKKDFTAEGFEVQRVLQAPGSSIIYVLGFLHSSEAVVYQIDSKSGEV 213

Query: 182 IKHSTETFTGGFSGELVSVSDDVLVTLDATRSNLVILSLRDGEIGIQQSPIAHLVDEFSG 241
           +   +  F GGFSGE+ SVS D +V LD+TRS LV +   DG+I  Q++PI+ LV++ SG
Sbjct: 214 VAQKSTVFPGGFSGEISSVSSDKVVVLDSTRSILVTIGFIDGDISFQKTPISDLVED-SG 273

Query: 242 SVEIVPSKLSGILAIEVGSVLILIRVKGEGELEVVDKIHGQATLSDSLLVSESQRAVALV 301
           + EI+   LS +LA++V    I + V  +G+LEVVD +  +  +SDSL V++ Q A A V
Sbjct: 274 TAEILSPLLSNMLAVKVNKRTIFVNVGDKGKLEVVDSLSDETAMSDSLPVADDQEAFASV 333

Query: 302 QHKGSYMHLSVKLIDNWSSDFLEENIVIDDQRGFVHKVFLNSYIRTDRSHGFRALIVMED 361
            H+GS +HL VKL+++ ++  L E I +D  RG VHKVF+N+YIRTDRS+GFRALIVMED
Sbjct: 334 HHEGSRIHLMVKLVNDLNNVLLRETIQMDQNRGRVHKVFMNNYIRTDRSNGFRALIVMED 393

Query: 362 HSLLLVQQGEIVWSREDGLASIINVVTSELPVEKKGVSIAKVENNLIEWLQGHLLKLKGT 421
           HSLLL+QQG IVWSRE+GLAS+ +V T+ELP+EK GVS+AKVE+ L EWL+GH+LKLKG+
Sbjct: 394 HSLLLLQQGAIVWSREEGLASVTDVTTAELPLEKDGVSVAKVEHTLFEWLKGHVLKLKGS 453

Query: 422 LMIASPEDVAAIQNMRLKSSDKSKLSRDHNGFRKLLIVLTKSGKLYALHSGDGRIVWSLL 481
           L++ASPEDV AIQ++R+KSS K+KL+RDHNGFRKL++ LT++GKL+ALH+GDGRIVWS+L
Sbjct: 454 LLLASPEDVVAIQDLRVKSSGKNKLTRDHNGFRKLILALTRAGKLFALHTGDGRIVWSML 513

Query: 482 LHPFCKSEAC-TPRWLNIYQWQDPHHRAMDENPSVLIVGRCGQSMGGPGFLSFVDTYTGK 541
           L+   +S++C  P  +++YQWQ PHH AMDENPSVL+VG+CG     PG LSFVD YTGK
Sbjct: 514 LNSPSQSQSCERPNGVSLYQWQVPHHHAMDENPSVLVVGKCGSDSSAPGVLSFVDVYTGK 573

Query: 542 EIGSSSQVHSVVQVIPLPLTDSTEQRLHILIDAESRAHLYPQTSEAISILQSEFSNIYWY 601
           EI SS   HSVVQV+PLP+TDS EQRLH++ D     HLYP+TSEA+SI Q EF N+YWY
Sbjct: 574 EISSSDIGHSVVQVMPLPITDSKEQRLHLIADTVGHVHLYPKTSEALSIFQREFQNVYWY 633

Query: 602 SVEADSGVIKGHALKRNCI-DILDDYCFESKDVWSIMLPSESEKIISTATRNLNEVVHTQ 661
           +VEAD G+I+GH +K +C  +  D+YCF ++++W+++ PSESEKIIST TR  NEVVHTQ
Sbjct: 634 TVEADDGIIRGHVMKGSCSGETADEYCFTTRELWTVVFPSESEKIISTLTRKPNEVVHTQ 693

Query: 662 AKVAADQDVMYKYISKNLLFLATVAPKSSGEIGTTTPEDSWLVVYLIDIVNGRILHRMTH 721
           AKV  DQD++YKY+S+NLLF+ATV+PK +GEIG+ TPE+S LVVYLID + GRILHR++H
Sbjct: 694 AKVNTDQDLLYKYVSRNLLFVATVSPKGAGEIGSVTPEESSLVVYLIDTITGRILHRLSH 753

Query: 722 HGSTGPVHAVFSENWVVYHYFNLKAHRYEMSVVEIYDQSRADNIDVWKLILGKHNLTSPI 781
            G  GPVHAVFSENWVVYHYFNL+AH+YE++VVEIYDQSRA+N +VWKLILGKHNLT+PI
Sbjct: 754 QGCQGPVHAVFSENWVVYHYFNLRAHKYEVTVVEIYDQSRAENKNVWKLILGKHNLTAPI 813

Query: 782 SSYSRPEILAKSQSYFFTHSVKAISVTSTSKGITSKQLLIGTINDQILALDKRYLDPRRS 841
           +SYSRPE+  KSQSYFF  SVK I+VTST+KGITSKQLLIGTI DQILALDKR++DPRR+
Sbjct: 814 TSYSRPEVFTKSQSYFFAQSVKTIAVTSTAKGITSKQLLIGTIGDQILALDKRFVDPRRT 873

Query: 842 INPSQAEREEGVIPLTDSLPIIPQTYATHALQVEGLRGVVTVPAKLESTTLAFAYGVDLF 901
           +NPSQAE+EEG+IPLTD+LPIIPQ Y TH+ +VEGLRG+VT P+KLESTT  FAYGVDLF
Sbjct: 874 LNPSQAEKEEGIIPLTDTLPIIPQAYVTHSHKVEGLRGIVTAPSKLESTTHVFAYGVDLF 933

Query: 902 FTRITPSRTYDSLTEDFSYALLLLTIVALVVAIFVTWGLSERKELQDKWK 950
           +TR+ PS+TYDSLT+DFSYALLL+TIVALV AI++TW LSE+KEL +KW+
Sbjct: 934 YTRLAPSKTYDSLTDDFSYALLLITIVALVAAIYITWVLSEKKELSEKWR 982