MS001177.1 (mRNA) Bitter gourd (TR) v1

BLAST of MS001177.1 vs. NCBI nr
Match: XP_011649705.1 (protein DETOXIFICATION 48 [Cucumis sativus] >KGN62780.1 hypothetical protein Csa_022014 [Cucumis sativus])

HSP 1 Score: 944.1 bits (2439), Expect = 5.6e-271
Identity = 498/583 (85.42%), Postives = 515/583 (88.34%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLP-PNYKTNFMNAQTPNKNLDNN----------NNHLLKPVADDDQ 60
           MCNPKP+SPNNSSFLP  NYKTNFM   TPNKNLDNN          NN+LLKP   DDQ
Sbjct: 1   MCNPKPSSPNNSSFLPSTNYKTNFMTTTTPNKNLDNNNNNLNTHHHHNNNLLKP--SDDQ 60

Query: 61  LAQLHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI 120
           LAQLHRLPTLSEAVEE+KEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI
Sbjct: 61  LAQLHRLPTLSEAVEEMKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI 120

Query: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMK 180
           GFANITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMK
Sbjct: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMK 180

Query: 181 RILLWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240
           RILLWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH
Sbjct: 181 RILLWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240

Query: 241 IPLNFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAP 300
           +PLNFLLVVH K+GISGVAIAMVWFN NVFLFL SFVYFSGVYKDSWVSPSVDCLHGW P
Sbjct: 241 VPLNFLLVVHFKMGISGVAIAMVWFNLNVFLFLVSFVYFSGVYKDSWVSPSVDCLHGWTP 300

Query: 301 LLSLAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGV 360
           LLSLAIPTCVSVCLEWWWYEFMI+LCGLLVNPKATIASMGILIQTTSLVYVFPSSLS GV
Sbjct: 301 LLSLAIPTCVSVCLEWWWYEFMIMLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSFGV 360

Query: 361 STRVGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTA 420
           STRVGNELGANRPAKAR SMIVSL CA ALGVAAM FTTLMRHKWGRFFTDD+EILELTA
Sbjct: 361 STRVGNELGANRPAKARISMIVSLICAVALGVAAMVFTTLMRHKWGRFFTDDAEILELTA 420

Query: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFA 480
           VALPIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVK+GFA
Sbjct: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKMGFA 480

Query: 481 GLWIGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPP------PPLLPISSPS 540
           GLWIGLLAAQG CA +MIYVLCTTDW+VQVERA QLT+            PPLLPIS  S
Sbjct: 481 GLWIGLLAAQGTCALMMIYVLCTTDWMVQVERAMQLTMASSSSSSSSNSNPPLLPISLSS 540

Query: 541 QDGAQNFQIQMGKNGTKLDGNKTGNLEDILCSNYETDPLIPIP 567
                     MG+        K  NLE ILCSN+ET PLIP P
Sbjct: 541 SCSEDEEDDDMGRKNGSQSMKKIENLEQILCSNHETHPLIPTP 581

BLAST of MS001177.1 vs. NCBI nr
Match: XP_008444968.1 (PREDICTED: protein DETOXIFICATION 48 [Cucumis melo])

HSP 1 Score: 933.7 bits (2412), Expect = 7.5e-268
Identity = 497/588 (84.52%), Postives = 518/588 (88.10%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLP-PNYKTNFMNAQTPNKNLDNN----------NNHLLKPVADDDQ 60
           MCNPKP+SPNNSSFLP  NYKTNFM   TP KNLDNN          NN+LLKP   DDQ
Sbjct: 1   MCNPKPSSPNNSSFLPSTNYKTNFMTTTTPKKNLDNNKNNLNIHHHLNNNLLKP--SDDQ 60

Query: 61  LAQLHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI 120
           LAQLHR PTLSEAVEE+KEIGKISGPTAITGLLLYSRAMIS+LFLGYLGELELAGGSLSI
Sbjct: 61  LAQLHRFPTLSEAVEEMKEIGKISGPTAITGLLLYSRAMISVLFLGYLGELELAGGSLSI 120

Query: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMK 180
           GFANITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMK
Sbjct: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMK 180

Query: 181 RILLWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240
           RILLWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH
Sbjct: 181 RILLWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240

Query: 241 IPLNFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAP 300
           +PLNFLLVVH K+GISGVAIAMVWFN NVFLFL SFVYFSGVYKDSWVSPSVDCLHGW P
Sbjct: 241 VPLNFLLVVHFKMGISGVAIAMVWFNLNVFLFLVSFVYFSGVYKDSWVSPSVDCLHGWTP 300

Query: 301 LLSLAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGV 360
           LLSLAIPTCVSVCLEWWWYEFMI+LCGLLVNPKATIASMGILIQTTSLVYVFPSSLS GV
Sbjct: 301 LLSLAIPTCVSVCLEWWWYEFMIMLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSFGV 360

Query: 361 STRVGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTA 420
           STRVGNELGANRPAKAR SMIVSL CA ALGVAAM FTTLMRHKWGRFFTDD+EILELTA
Sbjct: 361 STRVGNELGANRPAKARISMIVSLICAVALGVAAMVFTTLMRHKWGRFFTDDAEILELTA 420

Query: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFA 480
           VALPIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVK+GFA
Sbjct: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKMGFA 480

Query: 481 GLWIGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPP----PPLLPIS----- 540
           GLWIGLLAAQG CA +MIYVLCTTDW+VQVERA QLT+          PPLLPIS     
Sbjct: 481 GLWIGLLAAQGTCALMMIYVLCTTDWMVQVERAMQLTMASSSSSSNCNPPLLPISVSSSS 540

Query: 541 --SPSQDGAQNFQIQMGKNGTKLDGNKTGNLEDILCSNYETDPLIPIP 567
             S  ++   N +   G NG  +   K  NLE ILCSN+ET PLIP P
Sbjct: 541 CCSEDEEDDDNGK-DGGSNGQSM--KKIENLEQILCSNHETHPLIPTP 583

BLAST of MS001177.1 vs. NCBI nr
Match: XP_038884730.1 (protein DETOXIFICATION 48 [Benincasa hispida])

HSP 1 Score: 928.3 bits (2398), Expect = 3.2e-266
Identity = 491/577 (85.10%), Postives = 514/577 (89.08%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNYKTNFMNAQTPNKNLDNNN------NHLLKPVADDDQLAQLH 60
           MCNPKP+SPNNSSFLP  YKTNFM A  PNKNLDNNN      N+LLKP   DDQL QLH
Sbjct: 1   MCNPKPSSPNNSSFLP--YKTNFMTA--PNKNLDNNNHNNHHINNLLKP--SDDQLTQLH 60

Query: 61  RLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANI 120
           R PTLSEAVEE+KEIGKISGPTAITGLLLYSRAMISMLFLG+LGELELA GSLSIGFANI
Sbjct: 61  RFPTLSEAVEEMKEIGKISGPTAITGLLLYSRAMISMLFLGHLGELELAAGSLSIGFANI 120

Query: 121 TGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLW 180
           TGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMKRILLW
Sbjct: 121 TGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMKRILLW 180

Query: 181 CGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNF 240
           CGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH+PLNF
Sbjct: 181 CGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHVPLNF 240

Query: 241 LLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLA 300
           LLVVH K+GISGVAIAMVWFN NVFLFL SFVYFSGVYKDSWV PSVDCLHGW PLLSLA
Sbjct: 241 LLVVHFKMGISGVAIAMVWFNLNVFLFLVSFVYFSGVYKDSWVPPSVDCLHGWTPLLSLA 300

Query: 301 IPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVG 360
           IPTCVSVCLEWWWYEFM +LCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVG
Sbjct: 301 IPTCVSVCLEWWWYEFMTMLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVG 360

Query: 361 NELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPI 420
           NELGANRPAKAR +MIVSL CA ALGVAAM FTTLMRHKWGRFFT+D+EILELTAVALPI
Sbjct: 361 NELGANRPAKARIAMIVSLACAVALGVAAMVFTTLMRHKWGRFFTNDAEILELTAVALPI 420

Query: 421 VGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIG 480
           VGLCELGNCPQTTGCGVLRGSARP  GANINLGSFYLVGFPVAILMGFVVKLGFAGLWIG
Sbjct: 421 VGLCELGNCPQTTGCGVLRGSARPATGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIG 480

Query: 481 LLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPI-----SSPSQDGAQN 540
           LLAAQG CA +M YVLC+TDW+VQVERA QLT+      PPLLPI     SS S+D  ++
Sbjct: 481 LLAAQGTCALMMFYVLCSTDWMVQVERAMQLTLASSNCNPPLLPISASSSSSCSEDDEED 540

Query: 541 FQIQMGKNGTKLDGNKTGNLEDILCSNYETDPLIPIP 567
              + G    K    K  NLE ILCSN+ETDPLIP P
Sbjct: 541 DDSKDGSQSMK----KIENLEQILCSNHETDPLIPTP 567

BLAST of MS001177.1 vs. NCBI nr
Match: XP_023545512.1 (protein DETOXIFICATION 48-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.5 bits (2388), Expect = 4.6e-265
Identity = 492/573 (85.86%), Postives = 516/573 (90.05%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNY--KTNFMNAQTPNKNLDN------NNNHLLKPVADDDQLAQ 60
           MCNPKP+        PPNY  KTN M A TPNKNLDN      +NN+LLKP   DDQLAQ
Sbjct: 1   MCNPKPS--------PPNYKTKTNLMTA-TPNKNLDNINCSNLHNNNLLKPA--DDQLAQ 60

Query: 61  LHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120
           LH  PTLSEAVEE+KEIGKISGPTA+TGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA
Sbjct: 61  LHTFPTLSEAVEEMKEIGKISGPTAVTGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120

Query: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRIL 180
           NITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMKRIL
Sbjct: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMKRIL 180

Query: 181 LWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPL 240
           LWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH+PL
Sbjct: 181 LWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHVPL 240

Query: 241 NFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300
           NFLLVVH K+GISGVAIAMVWFN NVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS
Sbjct: 241 NFLLVVHFKMGISGVAIAMVWFNLNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300

Query: 301 LAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360
           LAIPTCVSVCLEWWWYEFMI+LCGLL NPKATIASMGILIQTTSLVYVFPSSLSLGVSTR
Sbjct: 301 LAIPTCVSVCLEWWWYEFMIMLCGLLGNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360

Query: 361 VGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVAL 420
           VGNELGANRPAKAR SMIVSL CAAALGVAAM FTTLMRHKWGRFFT+D+EILELTAVAL
Sbjct: 361 VGNELGANRPAKARISMIVSLACAAALGVAAMVFTTLMRHKWGRFFTNDAEILELTAVAL 420

Query: 421 PIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480
           PIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVKLGFAGLW
Sbjct: 421 PIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480

Query: 481 IGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPISSPSQDGAQNFQ- 540
           IGLLAAQG CA +MIYVLCTTDW+VQVERA QLT       PPLLPIS+ S   ++N + 
Sbjct: 481 IGLLAAQGSCAVMMIYVLCTTDWMVQVERAMQLTKASSNCNPPLLPISASSSSSSENHRS 540

Query: 541 IQMGKNGTKLDGNKTGNLEDILCSNYETDPLIP 565
            + GK+ T+    K  NLE ILCSN+ETDPLIP
Sbjct: 541 SENGKDETQ-SMKKMTNLEQILCSNHETDPLIP 561

BLAST of MS001177.1 vs. NCBI nr
Match: XP_022962254.1 (protein DETOXIFICATION 48-like [Cucurbita moschata])

HSP 1 Score: 920.2 bits (2377), Expect = 8.6e-264
Identity = 492/574 (85.71%), Postives = 516/574 (89.90%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNY--KTNFMNAQTPNKNLDN------NNNHLLKPVADDDQLAQ 60
           MCNPKP+        PPNY  KTN M A TPNKNLDN      +NN+LLKP   DDQLAQ
Sbjct: 1   MCNPKPS--------PPNYKTKTNLMTA-TPNKNLDNINCSNLHNNNLLKPA--DDQLAQ 60

Query: 61  LHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120
           LH  PTLSEAVEE+KEIGKISGPTA+TGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA
Sbjct: 61  LHTFPTLSEAVEEMKEIGKISGPTAVTGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120

Query: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRIL 180
           NITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMKRIL
Sbjct: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMKRIL 180

Query: 181 LWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPL 240
           LWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH+PL
Sbjct: 181 LWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHVPL 240

Query: 241 NFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300
           NFLLVVH K+GISGVAIAMVWFN NVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS
Sbjct: 241 NFLLVVHFKMGISGVAIAMVWFNLNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300

Query: 301 LAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360
           LAIPTCVSVCLEWWWYEFMI+LCGLL NPKATIASMGILIQTTSLVYVFPSSLSLGVSTR
Sbjct: 301 LAIPTCVSVCLEWWWYEFMIMLCGLLGNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360

Query: 361 VGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVAL 420
           VGNELGANRPAKAR SMIVSL CAAALGVAAM FTTLMRHKWGRFFT+D++ILELTAVAL
Sbjct: 361 VGNELGANRPAKARISMIVSLACAAALGVAAMVFTTLMRHKWGRFFTNDADILELTAVAL 420

Query: 421 PIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480
           PIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVKLGFAGLW
Sbjct: 421 PIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480

Query: 481 IGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPIS-SPSQDGAQNFQ 540
           IGLLAAQG CA +MIYVLCTTDW+VQVERA QLT       PPLLPIS S S   ++N +
Sbjct: 481 IGLLAAQGSCAVMMIYVLCTTDWMVQVERAMQLTKASSNCNPPLLPISASSSSSSSENHR 540

Query: 541 -IQMGKNGTKLDGNKTGNLEDILCSNYETDPLIP 565
             + GK+ T+    K  NLE ILCSN+ETDPLIP
Sbjct: 541 SSENGKDETQ-STKKMTNLEQILCSNHETDPLIP 562

BLAST of MS001177.1 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 1.1e-202
Identity = 374/521 (71.79%), Postives = 429/521 (82.34%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNYKTNFMNAQTPNKNLDNNNNHLLKPVADDDQLAQLHRLPTLS 60
           MCN KP+S  +SS L    KT+    +T     D +N H      D D L  L R P+  
Sbjct: 1   MCNSKPSSA-SSSLLSCKDKTHISKLET----CDTDNPH-YSEFRDTDSL-DLKRWPSFL 60

Query: 61  EAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVL 120
           E +EEVK IGKISGPTA+TGLL+YSRAMISMLFLGYLGELELAGGSLSIGFANITGYSV+
Sbjct: 61  EGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVI 120

Query: 121 SGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQDQE 180
           SGL+MGMEPICGQAYGAKQ K+LG+TLQRTVLLLL+ SVPISF WLNM+RILLWCGQD+E
Sbjct: 121 SGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEE 180

Query: 181 ISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLVVHL 240
           ISS+AQ F+LF+IPDL  LSLLHPLRIYLRTQ+ITLP+TY +A+SVLLH+PLN+LLVV L
Sbjct: 181 ISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKL 240

Query: 241 KLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIPTCVS 300
           ++G++GVAIAMV  N N+ + L+SFVYF+ V+ D+WV  ++D L GW+ LLSLAIPTCVS
Sbjct: 241 EMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVS 300

Query: 301 VCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNELGAN 360
           VCLEWWWYEFMI+LCGLL NP+AT+ASMGILIQTT+LVYVFPSSLSLGVSTR+ NELGA 
Sbjct: 301 VCLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAK 360

Query: 361 RPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVGLCEL 420
           RPAKAR SMI+SL CA ALG+ AM F  L+RH WGR FT D+EIL+LT++ALPIVGLCEL
Sbjct: 361 RPAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCEL 420

Query: 421 GNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLLAAQG 480
           GNCPQTTGCGVLRG ARPT+GANINLGSFY VG PVAIL GFV K GF GLW GLLAAQ 
Sbjct: 421 GNCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQA 480

Query: 481 CCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPISS 522
            CA LM+  L  TDW VQ ERA +LT +     PPLLPI+S
Sbjct: 481 TCASLMLCALLRTDWKVQAERAEELTSQTPGKSPPLLPIAS 514

BLAST of MS001177.1 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 3.0e-158
Identity = 284/448 (63.39%), Postives = 354/448 (79.02%), Query Frame = 0

Query: 59  LSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELE-LAGGSLSIGFANITGY 118
           LS +++E K I KIS P  +TGLLLYSR+MISMLFLG L +L  L+GGSL++GFANITGY
Sbjct: 26  LSSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGY 85

Query: 119 SVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQ 178
           S+LSGL++GMEPIC QA+GAK++K+LG+ LQRT LLLL  S+PIS +WLN+K+ILL+ GQ
Sbjct: 86  SLLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQ 145

Query: 179 DQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLV 238
           D+EIS+ A+ FILFS+PDLI  S LHP+RIYLR+QSITLPLTY +  +VLLHIP+N+LLV
Sbjct: 146 DEEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLV 205

Query: 239 VHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIPT 298
             L LG+ GVA+  +W N N+  FL  ++ FSGVY+ +W   S+DC  GW  L+ LAIP+
Sbjct: 206 SSLGLGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPS 265

Query: 299 CVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNEL 358
           CVSVCLEWWWYE MILLCGLL+NP+AT+ASMGILIQTT+L+Y+FPSSLS+ VSTRVGNEL
Sbjct: 266 CVSVCLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNEL 325

Query: 359 GANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVGL 418
           GAN+P KAR +    L  +  LG+ AM F  ++R+ W R FTD+ EI++LT++ LPI+GL
Sbjct: 326 GANQPDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGL 385

Query: 419 CELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLLA 478
           CELGNCPQTT CGVLRGSARP +GANINL  FY VG PVA+ + F     F GLW+GL A
Sbjct: 386 CELGNCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFA 445

Query: 479 AQGCCAFLMIYVLCTTDWLVQVERATQL 506
           AQG C   M+ VL  TDW V+V RA +L
Sbjct: 446 AQGSCLISMLVVLARTDWEVEVHRAKEL 473

BLAST of MS001177.1 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 4.4e-146
Identity = 270/506 (53.36%), Postives = 359/506 (70.95%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNYKTNFMNAQTPNKNLDNNNNHLLKPVADDDQLAQLHRLPTLS 60
           MCNP  T+    S    +    F++  + N           +P   + +  +    P ++
Sbjct: 1   MCNPSTTTTTTGSENQESRTGLFLDLFSINS---------FEPTKRNLRHCENRGSPLMA 60

Query: 61  EAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVL 120
           EAV E K +  ++ P A+T L+LY R+ +SM FLG LG+LELA GSL+I FANITGYSVL
Sbjct: 61  EAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVL 120

Query: 121 SGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQDQE 180
           SGLA+GMEP+C QA+GA ++K+L +TL RTV+ LL   VPIS +W N+ +I ++  QD +
Sbjct: 121 SGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPD 180

Query: 181 ISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLVVHL 240
           I+ +AQT+++FS+PDL+  +LLHP+RIYLR Q I  P+T  S    + H+P N  LV +L
Sbjct: 181 IAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYL 240

Query: 241 KLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIPTCVS 300
           +LG++GVA+A    N  V  FL  +V+ SG++  +W  P+ DC  GWAPLL LA P+CVS
Sbjct: 241 RLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGPSCVS 300

Query: 301 VCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNELGAN 360
           VCLEWWWYE MI+LCGLLVNP++T+A+MG+LIQTTS +YVFPSSLS  VSTRVGNELGAN
Sbjct: 301 VCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGAN 360

Query: 361 RPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVGLCEL 420
           RP  A+ +  V++  AA  G+ A AF   +R+ WGR FT D EIL+LTA ALPI+GLCE+
Sbjct: 361 RPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEI 420

Query: 421 GNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLLAAQG 480
           GNCPQT GCGV+RG+ARP+  AN+NLG+FYLVG PVA+ +GF   +GF GLW+GLLAAQ 
Sbjct: 421 GNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQI 480

Query: 481 CCAFLMIYVLCTTDWLVQVERATQLT 507
            CA LM+YV+ TTDW  + ++A  LT
Sbjct: 481 SCAGLMMYVVGTTDWESEAKKAQTLT 497

BLAST of MS001177.1 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.2e-145
Identity = 269/484 (55.58%), Postives = 354/484 (73.14%), Query Frame = 0

Query: 27  QTPNKNLDNNNNHLLKPVADDDQLAQLHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSR 86
           +TP  N+ ++ N L K   +    A +   PT++E   E + +  ++ PT +  L+LY+R
Sbjct: 2   ETP--NIISHTNLLSKIDLEKQNPAPI--FPTITELKSEARSLFSLAFPTILAALILYAR 61

Query: 87  AMISMLFLGYLGELELAGGSLSIGFANITGYSVLSGLAMGMEPICGQAYGAKQWKILGIT 146
           + ISMLFLG++GELELAGGSL+I FANITGYSVL+GLA+GM+P+C QA+GA + K+L +T
Sbjct: 62  SAISMLFLGHIGELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFGAGRPKLLSLT 121

Query: 147 LQRTVLLLLTSSVPISFMWLNMKRILLWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLR 206
           LQRTVL LLTSSV I  +WLN+ +I+++  QD  ISS+AQT+IL SIPDL+  S LHPLR
Sbjct: 122 LQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDLLTNSFLHPLR 181

Query: 207 IYLRTQSITLPLTYCSALSVLLHIPLNFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFV 266
           IYLR Q IT PLT  +    + HIP+NF LV +L  G  GV++A    N  V +FL + V
Sbjct: 182 IYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNLLVVIFLVAHV 241

Query: 267 YFSGVYKDSWVSPSVDCLHGWAPLLSLAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIA 326
           + +G+++ +W  PS +C   W P+++LAIP+C+ VCLEWWWYE M +LCGLL++P   +A
Sbjct: 242 WIAGLHQPTWTRPSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCGLLIDPSTPVA 301

Query: 327 SMGILIQTTSLVYVFPSSLSLGVSTRVGNELGANRPAKARNSMIVSLGCAAALGVAAMAF 386
           SMGILIQTTSL+Y+FPSSL L VSTRVGNELG+NRP KAR S IV++  A  +G+ A AF
Sbjct: 302 SMGILIQTTSLLYIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFAGVMGLTASAF 361

Query: 387 TTLMRHKWGRFFTDDSEILELTAVALPIVGLCELGNCPQTTGCGVLRGSARPTIGANINL 446
              +   WG  FT+D  I++LTA ALPI+GLCELGNCPQT GCGV+RG+ARP++ ANINL
Sbjct: 362 AWGVSDVWGWIFTNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTARPSMAANINL 421

Query: 447 GSFYLVGFPVAILMGFVVKLGFAGLWIGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLT 506
           G+FYLVG PVA+ + F    GF GLW+GLLAAQ CCA +M+YV+ TTDW  +  RA +LT
Sbjct: 422 GAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWEKEAIRARKLT 481

Query: 507 IKGG 511
              G
Sbjct: 482 CTEG 481

BLAST of MS001177.1 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.7e-135
Identity = 253/454 (55.73%), Postives = 331/454 (72.91%), Query Frame = 0

Query: 59  LSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYS 118
           LS  + E   I KIS P  +TGL LY R+ +S+ FLG LG+  LAGGSL+  FANITGYS
Sbjct: 29  LSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYS 88

Query: 119 VLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQD 178
           + SGL MG+E IC QA+GA+++  +  +++R ++LLL +S+P++ +W+NM++ILL   QD
Sbjct: 89  LFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQD 148

Query: 179 QEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLVV 238
           ++++S A  F+L+S+PDL+  S LHPLR+YLRTQS TLPL+ C+ ++  LH+P+ F LV 
Sbjct: 149 KKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVS 208

Query: 239 HLKLGISGVAIAMVWFNFNVFLFLASFVYF----SGVYKDSWVSPSV--DCLHGWAPLLS 298
           +L LGI G+A++ V  NFN+  FL  ++ F      V +D  ++     D +  W  LL 
Sbjct: 209 YLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITEETCEDSVREWKKLLC 268

Query: 299 LAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 358
           LAIP+C+SVCLEWW YE MILLCG L++PKA++ASMGILIQ TSLVY+FP SLSLGVSTR
Sbjct: 269 LAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTR 328

Query: 359 VGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVAL 418
           VGNELG+N+P +AR + IV LG + ALG  A AFT  +R+ W  FFTDD EI++LTA+AL
Sbjct: 329 VGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMAL 388

Query: 419 PIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 478
           PIVGLCELGNCPQTTGCGVLRGSARP IGANIN  +FY VG PV  ++ F    GF GLW
Sbjct: 389 PIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLW 448

Query: 479 IGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLT 507
           +G+LAAQ  C   M+   C TDW ++ ERA  LT
Sbjct: 449 LGMLAAQITCVIGMMAATCRTDWELEAERAKVLT 482

BLAST of MS001177.1 vs. ExPASy TrEMBL
Match: A0A0A0LLF9 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_2G372760 PE=3 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.7e-271
Identity = 498/583 (85.42%), Postives = 515/583 (88.34%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLP-PNYKTNFMNAQTPNKNLDNN----------NNHLLKPVADDDQ 60
           MCNPKP+SPNNSSFLP  NYKTNFM   TPNKNLDNN          NN+LLKP   DDQ
Sbjct: 1   MCNPKPSSPNNSSFLPSTNYKTNFMTTTTPNKNLDNNNNNLNTHHHHNNNLLKP--SDDQ 60

Query: 61  LAQLHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI 120
           LAQLHRLPTLSEAVEE+KEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI
Sbjct: 61  LAQLHRLPTLSEAVEEMKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI 120

Query: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMK 180
           GFANITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMK
Sbjct: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMK 180

Query: 181 RILLWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240
           RILLWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH
Sbjct: 181 RILLWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240

Query: 241 IPLNFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAP 300
           +PLNFLLVVH K+GISGVAIAMVWFN NVFLFL SFVYFSGVYKDSWVSPSVDCLHGW P
Sbjct: 241 VPLNFLLVVHFKMGISGVAIAMVWFNLNVFLFLVSFVYFSGVYKDSWVSPSVDCLHGWTP 300

Query: 301 LLSLAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGV 360
           LLSLAIPTCVSVCLEWWWYEFMI+LCGLLVNPKATIASMGILIQTTSLVYVFPSSLS GV
Sbjct: 301 LLSLAIPTCVSVCLEWWWYEFMIMLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSFGV 360

Query: 361 STRVGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTA 420
           STRVGNELGANRPAKAR SMIVSL CA ALGVAAM FTTLMRHKWGRFFTDD+EILELTA
Sbjct: 361 STRVGNELGANRPAKARISMIVSLICAVALGVAAMVFTTLMRHKWGRFFTDDAEILELTA 420

Query: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFA 480
           VALPIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVK+GFA
Sbjct: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKMGFA 480

Query: 481 GLWIGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPP------PPLLPISSPS 540
           GLWIGLLAAQG CA +MIYVLCTTDW+VQVERA QLT+            PPLLPIS  S
Sbjct: 481 GLWIGLLAAQGTCALMMIYVLCTTDWMVQVERAMQLTMASSSSSSSSNSNPPLLPISLSS 540

Query: 541 QDGAQNFQIQMGKNGTKLDGNKTGNLEDILCSNYETDPLIPIP 567
                     MG+        K  NLE ILCSN+ET PLIP P
Sbjct: 541 SCSEDEEDDDMGRKNGSQSMKKIENLEQILCSNHETHPLIPTP 581

BLAST of MS001177.1 vs. ExPASy TrEMBL
Match: A0A1S3BB46 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103488152 PE=3 SV=1)

HSP 1 Score: 933.7 bits (2412), Expect = 3.6e-268
Identity = 497/588 (84.52%), Postives = 518/588 (88.10%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLP-PNYKTNFMNAQTPNKNLDNN----------NNHLLKPVADDDQ 60
           MCNPKP+SPNNSSFLP  NYKTNFM   TP KNLDNN          NN+LLKP   DDQ
Sbjct: 1   MCNPKPSSPNNSSFLPSTNYKTNFMTTTTPKKNLDNNKNNLNIHHHLNNNLLKP--SDDQ 60

Query: 61  LAQLHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSI 120
           LAQLHR PTLSEAVEE+KEIGKISGPTAITGLLLYSRAMIS+LFLGYLGELELAGGSLSI
Sbjct: 61  LAQLHRFPTLSEAVEEMKEIGKISGPTAITGLLLYSRAMISVLFLGYLGELELAGGSLSI 120

Query: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMK 180
           GFANITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMK
Sbjct: 121 GFANITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMK 180

Query: 181 RILLWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240
           RILLWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH
Sbjct: 181 RILLWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH 240

Query: 241 IPLNFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAP 300
           +PLNFLLVVH K+GISGVAIAMVWFN NVFLFL SFVYFSGVYKDSWVSPSVDCLHGW P
Sbjct: 241 VPLNFLLVVHFKMGISGVAIAMVWFNLNVFLFLVSFVYFSGVYKDSWVSPSVDCLHGWTP 300

Query: 301 LLSLAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGV 360
           LLSLAIPTCVSVCLEWWWYEFMI+LCGLLVNPKATIASMGILIQTTSLVYVFPSSLS GV
Sbjct: 301 LLSLAIPTCVSVCLEWWWYEFMIMLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSFGV 360

Query: 361 STRVGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTA 420
           STRVGNELGANRPAKAR SMIVSL CA ALGVAAM FTTLMRHKWGRFFTDD+EILELTA
Sbjct: 361 STRVGNELGANRPAKARISMIVSLICAVALGVAAMVFTTLMRHKWGRFFTDDAEILELTA 420

Query: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFA 480
           VALPIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVK+GFA
Sbjct: 421 VALPIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKMGFA 480

Query: 481 GLWIGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPP----PPLLPIS----- 540
           GLWIGLLAAQG CA +MIYVLCTTDW+VQVERA QLT+          PPLLPIS     
Sbjct: 481 GLWIGLLAAQGTCALMMIYVLCTTDWMVQVERAMQLTMASSSSSSNCNPPLLPISVSSSS 540

Query: 541 --SPSQDGAQNFQIQMGKNGTKLDGNKTGNLEDILCSNYETDPLIPIP 567
             S  ++   N +   G NG  +   K  NLE ILCSN+ET PLIP P
Sbjct: 541 CCSEDEEDDDNGK-DGGSNGQSM--KKIENLEQILCSNHETHPLIPTP 583

BLAST of MS001177.1 vs. ExPASy TrEMBL
Match: A0A6J1K6J3 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111492116 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 4.2e-264
Identity = 490/572 (85.66%), Postives = 515/572 (90.03%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNY--KTNFMNAQTPNKNLDN------NNNHLLKPVADDDQLAQ 60
           MCNPKP+        PPNY  KTN M A TPNKNLDN      +NN+LLKP   DDQLAQ
Sbjct: 1   MCNPKPS--------PPNYKTKTNLMTA-TPNKNLDNINCSNLHNNNLLKPA--DDQLAQ 60

Query: 61  LHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120
           LH  PTLSEAVEE+KEIGKISGPTA+TGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA
Sbjct: 61  LHTFPTLSEAVEEMKEIGKISGPTAVTGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120

Query: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRIL 180
           NITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMKRIL
Sbjct: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMKRIL 180

Query: 181 LWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPL 240
           LWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH+PL
Sbjct: 181 LWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHVPL 240

Query: 241 NFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300
           NFLLVVH K+GISGVAIAMVWFN NVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS
Sbjct: 241 NFLLVVHFKMGISGVAIAMVWFNLNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300

Query: 301 LAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360
           LAIPTCVSVCLEWWWYEFMI+LCGLL NPKATIASMGILIQTTSLVYVFPSSLSLGVSTR
Sbjct: 301 LAIPTCVSVCLEWWWYEFMIMLCGLLGNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360

Query: 361 VGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVAL 420
           VGNELGANRPAKAR SMIVSL CAAALGVAAM FTTLMRHKWGRFFT+D++ILELTAVAL
Sbjct: 361 VGNELGANRPAKARISMIVSLACAAALGVAAMVFTTLMRHKWGRFFTNDADILELTAVAL 420

Query: 421 PIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480
           PIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVKLGFAGLW
Sbjct: 421 PIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480

Query: 481 IGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPISSPSQDGAQNFQI 540
           IGLLAAQG CA +MIYVLCTTDW+VQVERA QLT       PPLLPIS+ S +  ++   
Sbjct: 481 IGLLAAQGSCAVMMIYVLCTTDWMVQVERAMQLTKASSNCNPPLLPISASSSENLRS--S 540

Query: 541 QMGKNGTKLDGNKTGNLEDILCSNYETDPLIP 565
           + GK+ T+    K  NLE ILCSN+ETDPLIP
Sbjct: 541 ENGKDETQ-SMKKMTNLEQILCSNHETDPLIP 558

BLAST of MS001177.1 vs. ExPASy TrEMBL
Match: A0A6J1HGJ5 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111462758 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 4.2e-264
Identity = 492/574 (85.71%), Postives = 516/574 (89.90%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNY--KTNFMNAQTPNKNLDN------NNNHLLKPVADDDQLAQ 60
           MCNPKP+        PPNY  KTN M A TPNKNLDN      +NN+LLKP   DDQLAQ
Sbjct: 1   MCNPKPS--------PPNYKTKTNLMTA-TPNKNLDNINCSNLHNNNLLKPA--DDQLAQ 60

Query: 61  LHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120
           LH  PTLSEAVEE+KEIGKISGPTA+TGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA
Sbjct: 61  LHTFPTLSEAVEEMKEIGKISGPTAVTGLLLYSRAMISMLFLGYLGELELAGGSLSIGFA 120

Query: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRIL 180
           NITGYSVLSGLAMGMEPICGQAYGAKQWK+LGITLQRTVLLLLTSSVPISFMWLNMKRIL
Sbjct: 121 NITGYSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRTVLLLLTSSVPISFMWLNMKRIL 180

Query: 181 LWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPL 240
           LWCGQD+EIS++AQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLH+PL
Sbjct: 181 LWCGQDEEISTVAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHVPL 240

Query: 241 NFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300
           NFLLVVH K+GISGVAIAMVWFN NVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS
Sbjct: 241 NFLLVVHFKMGISGVAIAMVWFNLNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLS 300

Query: 301 LAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360
           LAIPTCVSVCLEWWWYEFMI+LCGLL NPKATIASMGILIQTTSLVYVFPSSLSLGVSTR
Sbjct: 301 LAIPTCVSVCLEWWWYEFMIMLCGLLGNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 360

Query: 361 VGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVAL 420
           VGNELGANRPAKAR SMIVSL CAAALGVAAM FTTLMRHKWGRFFT+D++ILELTAVAL
Sbjct: 361 VGNELGANRPAKARISMIVSLACAAALGVAAMVFTTLMRHKWGRFFTNDADILELTAVAL 420

Query: 421 PIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480
           PIVGLCELGNCPQTTGCGVLRGSARPT GANINLGSFYLVGFPVAILMGFVVKLGFAGLW
Sbjct: 421 PIVGLCELGNCPQTTGCGVLRGSARPTTGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 480

Query: 481 IGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPIS-SPSQDGAQNFQ 540
           IGLLAAQG CA +MIYVLCTTDW+VQVERA QLT       PPLLPIS S S   ++N +
Sbjct: 481 IGLLAAQGSCAVMMIYVLCTTDWMVQVERAMQLTKASSNCNPPLLPISASSSSSSSENHR 540

Query: 541 -IQMGKNGTKLDGNKTGNLEDILCSNYETDPLIP 565
             + GK+ T+    K  NLE ILCSN+ETDPLIP
Sbjct: 541 SSENGKDETQ-STKKMTNLEQILCSNHETDPLIP 562

BLAST of MS001177.1 vs. ExPASy TrEMBL
Match: A0A6J1KKX1 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111496176 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 6.0e-263
Identity = 482/569 (84.71%), Postives = 509/569 (89.46%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNYKTNFMNAQTPNKNLDNNN----NHLLKPVADDDQLAQLHRL 60
           MCNPKP+SPNNSSFLPPNYK NFM   TPNK+LDN+N    N+LLKP   DDQLAQLHR 
Sbjct: 1   MCNPKPSSPNNSSFLPPNYKANFM--ATPNKSLDNHNLNNKNNLLKPA--DDQLAQLHRF 60

Query: 61  PTLSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITG 120
           PT+SEAVEE+KEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELA GSLSIGFANITG
Sbjct: 61  PTVSEAVEEMKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAAGSLSIGFANITG 120

Query: 121 YSVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCG 180
           YSVLSGLAMGMEPICGQAYGAKQWK+LGITLQR VLLLLTSSVPISFMWLNM+RILLWCG
Sbjct: 121 YSVLSGLAMGMEPICGQAYGAKQWKLLGITLQRAVLLLLTSSVPISFMWLNMRRILLWCG 180

Query: 181 QDQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLL 240
           QD++ISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSA SVLLHIPLNFLL
Sbjct: 181 QDEQISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSAFSVLLHIPLNFLL 240

Query: 241 VVHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIP 300
           VVH K+GISGVAIAM+WFNFNVF FL SFVYFSGVYKDSWVSP+ DCLHGWAPLLSLAIP
Sbjct: 241 VVHFKMGISGVAIAMIWFNFNVFFFLVSFVYFSGVYKDSWVSPTQDCLHGWAPLLSLAIP 300

Query: 301 TCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNE 360
           TCVSVCLEWWWYEFMI+LCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNE
Sbjct: 301 TCVSVCLEWWWYEFMIMLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNE 360

Query: 361 LGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVG 420
           LGANRPAKAR SMIVSL CAA LGV AM FTTL+RHKWGRFFT+D+EILELT+VALPIVG
Sbjct: 361 LGANRPAKARLSMIVSLLCAAVLGVLAMVFTTLIRHKWGRFFTNDTEILELTSVALPIVG 420

Query: 421 LCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLL 480
           LCELGNCPQTTGCGVLRG+ARPTIGANINLGSFYLVGFPVAI+MGFVVKLGFAGLWIGLL
Sbjct: 421 LCELGNCPQTTGCGVLRGTARPTIGANINLGSFYLVGFPVAIIMGFVVKLGFAGLWIGLL 480

Query: 481 AAQGCCAFLMIYVLCTTDWLVQVERATQLTIKGGPP-PPPLLPISSPSQDGAQNFQIQMG 540
           AAQG CA +MIYVL TTDW+ Q ERA QLT  G     PPLLP+SS S+D          
Sbjct: 481 AAQGSCALMMIYVLSTTDWIAQAERAMQLTKVGSSNCNPPLLPLSSSSED---------- 540

Query: 541 KNGTKLDGNKTGNLEDILCSNYETDPLIP 565
                L   K  NLE+ LCSN+ETD L+P
Sbjct: 541 ----DLSVKKIENLEENLCSNHETDLLLP 551

BLAST of MS001177.1 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 707.6 bits (1825), Expect = 8.1e-204
Identity = 374/521 (71.79%), Postives = 429/521 (82.34%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNYKTNFMNAQTPNKNLDNNNNHLLKPVADDDQLAQLHRLPTLS 60
           MCN KP+S  +SS L    KT+    +T     D +N H      D D L  L R P+  
Sbjct: 1   MCNSKPSSA-SSSLLSCKDKTHISKLET----CDTDNPH-YSEFRDTDSL-DLKRWPSFL 60

Query: 61  EAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVL 120
           E +EEVK IGKISGPTA+TGLL+YSRAMISMLFLGYLGELELAGGSLSIGFANITGYSV+
Sbjct: 61  EGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVI 120

Query: 121 SGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQDQE 180
           SGL+MGMEPICGQAYGAKQ K+LG+TLQRTVLLLL+ SVPISF WLNM+RILLWCGQD+E
Sbjct: 121 SGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEE 180

Query: 181 ISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLVVHL 240
           ISS+AQ F+LF+IPDL  LSLLHPLRIYLRTQ+ITLP+TY +A+SVLLH+PLN+LLVV L
Sbjct: 181 ISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKL 240

Query: 241 KLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIPTCVS 300
           ++G++GVAIAMV  N N+ + L+SFVYF+ V+ D+WV  ++D L GW+ LLSLAIPTCVS
Sbjct: 241 EMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVS 300

Query: 301 VCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNELGAN 360
           VCLEWWWYEFMI+LCGLL NP+AT+ASMGILIQTT+LVYVFPSSLSLGVSTR+ NELGA 
Sbjct: 301 VCLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAK 360

Query: 361 RPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVGLCEL 420
           RPAKAR SMI+SL CA ALG+ AM F  L+RH WGR FT D+EIL+LT++ALPIVGLCEL
Sbjct: 361 RPAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCEL 420

Query: 421 GNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLLAAQG 480
           GNCPQTTGCGVLRG ARPT+GANINLGSFY VG PVAIL GFV K GF GLW GLLAAQ 
Sbjct: 421 GNCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQA 480

Query: 481 CCAFLMIYVLCTTDWLVQVERATQLTIKGGPPPPPLLPISS 522
            CA LM+  L  TDW VQ ERA +LT +     PPLLPI+S
Sbjct: 481 TCASLMLCALLRTDWKVQAERAEELTSQTPGKSPPLLPIAS 514

BLAST of MS001177.1 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 560.1 bits (1442), Expect = 2.1e-159
Identity = 284/448 (63.39%), Postives = 354/448 (79.02%), Query Frame = 0

Query: 59  LSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELE-LAGGSLSIGFANITGY 118
           LS +++E K I KIS P  +TGLLLYSR+MISMLFLG L +L  L+GGSL++GFANITGY
Sbjct: 26  LSSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGY 85

Query: 119 SVLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQ 178
           S+LSGL++GMEPIC QA+GAK++K+LG+ LQRT LLLL  S+PIS +WLN+K+ILL+ GQ
Sbjct: 86  SLLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQ 145

Query: 179 DQEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLV 238
           D+EIS+ A+ FILFS+PDLI  S LHP+RIYLR+QSITLPLTY +  +VLLHIP+N+LLV
Sbjct: 146 DEEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLV 205

Query: 239 VHLKLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIPT 298
             L LG+ GVA+  +W N N+  FL  ++ FSGVY+ +W   S+DC  GW  L+ LAIP+
Sbjct: 206 SSLGLGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPS 265

Query: 299 CVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNEL 358
           CVSVCLEWWWYE MILLCGLL+NP+AT+ASMGILIQTT+L+Y+FPSSLS+ VSTRVGNEL
Sbjct: 266 CVSVCLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNEL 325

Query: 359 GANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVGL 418
           GAN+P KAR +    L  +  LG+ AM F  ++R+ W R FTD+ EI++LT++ LPI+GL
Sbjct: 326 GANQPDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGL 385

Query: 419 CELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLLA 478
           CELGNCPQTT CGVLRGSARP +GANINL  FY VG PVA+ + F     F GLW+GL A
Sbjct: 386 CELGNCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFA 445

Query: 479 AQGCCAFLMIYVLCTTDWLVQVERATQL 506
           AQG C   M+ VL  TDW V+V RA +L
Sbjct: 446 AQGSCLISMLVVLARTDWEVEVHRAKEL 473

BLAST of MS001177.1 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 519.6 bits (1337), Expect = 3.1e-147
Identity = 270/506 (53.36%), Postives = 359/506 (70.95%), Query Frame = 0

Query: 1   MCNPKPTSPNNSSFLPPNYKTNFMNAQTPNKNLDNNNNHLLKPVADDDQLAQLHRLPTLS 60
           MCNP  T+    S    +    F++  + N           +P   + +  +    P ++
Sbjct: 1   MCNPSTTTTTTGSENQESRTGLFLDLFSINS---------FEPTKRNLRHCENRGSPLMA 60

Query: 61  EAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVL 120
           EAV E K +  ++ P A+T L+LY R+ +SM FLG LG+LELA GSL+I FANITGYSVL
Sbjct: 61  EAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVL 120

Query: 121 SGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQDQE 180
           SGLA+GMEP+C QA+GA ++K+L +TL RTV+ LL   VPIS +W N+ +I ++  QD +
Sbjct: 121 SGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPD 180

Query: 181 ISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLVVHL 240
           I+ +AQT+++FS+PDL+  +LLHP+RIYLR Q I  P+T  S    + H+P N  LV +L
Sbjct: 181 IAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYL 240

Query: 241 KLGISGVAIAMVWFNFNVFLFLASFVYFSGVYKDSWVSPSVDCLHGWAPLLSLAIPTCVS 300
           +LG++GVA+A    N  V  FL  +V+ SG++  +W  P+ DC  GWAPLL LA P+CVS
Sbjct: 241 RLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGPSCVS 300

Query: 301 VCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTRVGNELGAN 360
           VCLEWWWYE MI+LCGLLVNP++T+A+MG+LIQTTS +YVFPSSLS  VSTRVGNELGAN
Sbjct: 301 VCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGAN 360

Query: 361 RPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVALPIVGLCEL 420
           RP  A+ +  V++  AA  G+ A AF   +R+ WGR FT D EIL+LTA ALPI+GLCE+
Sbjct: 361 RPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEI 420

Query: 421 GNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLWIGLLAAQG 480
           GNCPQT GCGV+RG+ARP+  AN+NLG+FYLVG PVA+ +GF   +GF GLW+GLLAAQ 
Sbjct: 421 GNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQI 480

Query: 481 CCAFLMIYVLCTTDWLVQVERATQLT 507
            CA LM+YV+ TTDW  + ++A  LT
Sbjct: 481 SCAGLMMYVVGTTDWESEAKKAQTLT 497

BLAST of MS001177.1 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 517.3 bits (1331), Expect = 1.6e-146
Identity = 269/484 (55.58%), Postives = 354/484 (73.14%), Query Frame = 0

Query: 27  QTPNKNLDNNNNHLLKPVADDDQLAQLHRLPTLSEAVEEVKEIGKISGPTAITGLLLYSR 86
           +TP  N+ ++ N L K   +    A +   PT++E   E + +  ++ PT +  L+LY+R
Sbjct: 2   ETP--NIISHTNLLSKIDLEKQNPAPI--FPTITELKSEARSLFSLAFPTILAALILYAR 61

Query: 87  AMISMLFLGYLGELELAGGSLSIGFANITGYSVLSGLAMGMEPICGQAYGAKQWKILGIT 146
           + ISMLFLG++GELELAGGSL+I FANITGYSVL+GLA+GM+P+C QA+GA + K+L +T
Sbjct: 62  SAISMLFLGHIGELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFGAGRPKLLSLT 121

Query: 147 LQRTVLLLLTSSVPISFMWLNMKRILLWCGQDQEISSMAQTFILFSIPDLIFLSLLHPLR 206
           LQRTVL LLTSSV I  +WLN+ +I+++  QD  ISS+AQT+IL SIPDL+  S LHPLR
Sbjct: 122 LQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDLLTNSFLHPLR 181

Query: 207 IYLRTQSITLPLTYCSALSVLLHIPLNFLLVVHLKLGISGVAIAMVWFNFNVFLFLASFV 266
           IYLR Q IT PLT  +    + HIP+NF LV +L  G  GV++A    N  V +FL + V
Sbjct: 182 IYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNLLVVIFLVAHV 241

Query: 267 YFSGVYKDSWVSPSVDCLHGWAPLLSLAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIA 326
           + +G+++ +W  PS +C   W P+++LAIP+C+ VCLEWWWYE M +LCGLL++P   +A
Sbjct: 242 WIAGLHQPTWTRPSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCGLLIDPSTPVA 301

Query: 327 SMGILIQTTSLVYVFPSSLSLGVSTRVGNELGANRPAKARNSMIVSLGCAAALGVAAMAF 386
           SMGILIQTTSL+Y+FPSSL L VSTRVGNELG+NRP KAR S IV++  A  +G+ A AF
Sbjct: 302 SMGILIQTTSLLYIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFAGVMGLTASAF 361

Query: 387 TTLMRHKWGRFFTDDSEILELTAVALPIVGLCELGNCPQTTGCGVLRGSARPTIGANINL 446
              +   WG  FT+D  I++LTA ALPI+GLCELGNCPQT GCGV+RG+ARP++ ANINL
Sbjct: 362 AWGVSDVWGWIFTNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTARPSMAANINL 421

Query: 447 GSFYLVGFPVAILMGFVVKLGFAGLWIGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLT 506
           G+FYLVG PVA+ + F    GF GLW+GLLAAQ CCA +M+YV+ TTDW  +  RA +LT
Sbjct: 422 GAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWEKEAIRARKLT 481

Query: 507 IKGG 511
              G
Sbjct: 482 CTEG 481

BLAST of MS001177.1 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.9e-136
Identity = 253/454 (55.73%), Postives = 331/454 (72.91%), Query Frame = 0

Query: 59  LSEAVEEVKEIGKISGPTAITGLLLYSRAMISMLFLGYLGELELAGGSLSIGFANITGYS 118
           LS  + E   I KIS P  +TGL LY R+ +S+ FLG LG+  LAGGSL+  FANITGYS
Sbjct: 29  LSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYS 88

Query: 119 VLSGLAMGMEPICGQAYGAKQWKILGITLQRTVLLLLTSSVPISFMWLNMKRILLWCGQD 178
           + SGL MG+E IC QA+GA+++  +  +++R ++LLL +S+P++ +W+NM++ILL   QD
Sbjct: 89  LFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQD 148

Query: 179 QEISSMAQTFILFSIPDLIFLSLLHPLRIYLRTQSITLPLTYCSALSVLLHIPLNFLLVV 238
           ++++S A  F+L+S+PDL+  S LHPLR+YLRTQS TLPL+ C+ ++  LH+P+ F LV 
Sbjct: 149 KKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVS 208

Query: 239 HLKLGISGVAIAMVWFNFNVFLFLASFVYF----SGVYKDSWVSPSV--DCLHGWAPLLS 298
           +L LGI G+A++ V  NFN+  FL  ++ F      V +D  ++     D +  W  LL 
Sbjct: 209 YLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITEETCEDSVREWKKLLC 268

Query: 299 LAIPTCVSVCLEWWWYEFMILLCGLLVNPKATIASMGILIQTTSLVYVFPSSLSLGVSTR 358
           LAIP+C+SVCLEWW YE MILLCG L++PKA++ASMGILIQ TSLVY+FP SLSLGVSTR
Sbjct: 269 LAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTR 328

Query: 359 VGNELGANRPAKARNSMIVSLGCAAALGVAAMAFTTLMRHKWGRFFTDDSEILELTAVAL 418
           VGNELG+N+P +AR + IV LG + ALG  A AFT  +R+ W  FFTDD EI++LTA+AL
Sbjct: 329 VGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMAL 388

Query: 419 PIVGLCELGNCPQTTGCGVLRGSARPTIGANINLGSFYLVGFPVAILMGFVVKLGFAGLW 478
           PIVGLCELGNCPQTTGCGVLRGSARP IGANIN  +FY VG PV  ++ F    GF GLW
Sbjct: 389 PIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLW 448

Query: 479 IGLLAAQGCCAFLMIYVLCTTDWLVQVERATQLT 507
           +G+LAAQ  C   M+   C TDW ++ ERA  LT
Sbjct: 449 LGMLAAQITCVIGMMAATCRTDWELEAERAKVLT 482