MELO3C025743.1 (mRNA) Melon (DHL92) v4

BLAST of MELO3C025743.1 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 4.8e-20
Identity = 75/286 (26.22%), Postives = 125/286 (43.71%), Query Frame = 0

Query: 282 KLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEV 341
           KL  DA  P V +      +K  N +C+ICLED   ++ FA+  C+HRYC  C K ++E+
Sbjct: 113 KLCIDAGVPNVMKLNATIVEKSGNVSCLICLEDYPPTQTFALI-CNHRYCLPCYKNYLEI 172

Query: 342 KLLQG---LVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKEASIPVSEKV-YCPYP 401
           K+ +G   +   CP   CK  ++ D+  + ++P+        I ++ +  + +V +CP P
Sbjct: 173 KVSEGPECIYTPCPAPKCKVIVHQDAFKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAP 232

Query: 402 RCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNL 461
            C               ++S        +C +      +NCK  +     CNDY+  +++
Sbjct: 233 GC---------------IYSI-------RCDRKERKEAVNCKCGFQYCFNCNDYEIGDHM 292

Query: 462 PTED-------VKLKSLASTCLW-----RQCVKCNHMIELAEGCYHMTCR-----CGHEF 521
           P           K    +    W     ++C +C   IE   GC HMTCR     CG EF
Sbjct: 293 PCPCSQVDKWLQKASDESENVTWMLANTKKCPECRSPIEKNGGCMHMTCRKNAGGCGFEF 352

Query: 522 CYKCGAEWKNKKATC----SCPLWAENHIWHDDDDDRDSDHDDQYY 543
           C+ C   W    +T     +C  + ++    DDD   D+  + + Y
Sbjct: 353 CWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAHDAKTELEAY 375

BLAST of MELO3C025743.1 vs. ExPASy Swiss-Prot
Match: F4KGU4 (ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10370 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 9.4e-16
Identity = 61/234 (26.07%), Postives = 97/234 (41.45%), Query Frame = 0

Query: 278  KYAFKLARD--AIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCM 337
            K   +LAR+  A+G +  + EV          C ICL + D    ++++GCSH +C +C+
Sbjct: 1541 KMVNELAREKSALGEKPDEIEV---------ECPICLSEVDDG--YSLEGCSHLFCKACL 1600

Query: 338  KQHVEVKL--LQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKEASIPVSEKV- 397
             +  E  +         C H  C   + +      L+ + +  L      + +  S+   
Sbjct: 1601 LEQFEASMRNFDAFPILCSHIDCGAPIVLADMRALLSQEKLDELFSASLSSFVTSSDGKF 1660

Query: 398  -YCPYPRCSALMTKVEVLTYTKDVFSAANQSGVR-KCMKCHGLFCINCKVPWHNRITCND 457
             +C  P C ++              +   +SG    C  CH   C  C + +H  ITC  
Sbjct: 1661 RFCSTPDCPSVYR-----------VAGPQESGEPFICGACHSEICTRCHLEYHPLITCER 1720

Query: 458  YKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKC 505
            YK+    P  D+ LK  A     ++C  C   IE  +GC HM CRCG   C+ C
Sbjct: 1721 YKKFKENP--DLSLKDWAKGKNVKECPICKSTIEKTDGCNHMKCRCGKHICWTC 1750

BLAST of MELO3C025743.1 vs. ExPASy Swiss-Prot
Match: P0CE10 (ATP-dependent RNA helicase DEAH11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01020 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.6e-15
Identity = 54/202 (26.73%), Postives = 84/202 (41.58%), Query Frame = 0

Query: 308  CVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKL--LQGLVPKCPHDGCKFDLNVDSC 367
            C ICL + D    ++++GCSH +C +C+ +  E  +         C H  C   + V   
Sbjct: 1561 CPICLSEVDDG--YSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1620

Query: 368  AKFLTPKDMATLRQRIKEASIPVSEKV--YCPYPRCSALMTKVEVLTYTKDVFSAANQSG 427
               L+ + +  L      A +  S+    +C  P C ++              +   +SG
Sbjct: 1621 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIYR-----------VAGPQESG 1680

Query: 428  VR-KCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHM 487
                C  CH   C  C + +H  ITC  YK+    P  D+ LK  A     ++C  C   
Sbjct: 1681 EPFICGACHSETCTRCHLEYHPLITCERYKKFKENP--DLSLKDWAKGKDVKECPICKST 1740

Query: 488  IELAEGCYHMTCRCGHEFCYKC 505
            IE  +GC H+ CRCG   C+ C
Sbjct: 1741 IEKTDGCNHLQCRCGKHICWTC 1747

BLAST of MELO3C025743.1 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 85.1 bits (209), Expect = 2.7e-15
Identity = 61/212 (28.77%), Postives = 90/212 (42.45%), Query Frame = 0

Query: 308 CVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVP-KCPHDGCKFDLNVDSCA 367
           C +CLED  +     +  C    C  C+K ++  ++  G V  KCP   C   L   +  
Sbjct: 263 CRVCLEDKPIK---PLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEETTVV 322

Query: 368 KFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMT---KVEVLTYTKDVFSAANQSG 427
             LT +D    +  ++   I  S K   P P+C    T   K  + T ++       Q  
Sbjct: 323 YNLTHEDSIKYKYFLELGRIDSSTK---PCPQCKHFTTFKKKGHIPTPSRSESKYKIQ-- 382

Query: 428 VRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQ-----CVK 487
              C  C  ++C  C  PWH  + C +YK+ + L      L+  AS     Q     C K
Sbjct: 383 ---CPTCQFVWCFKCHSPWHEGVNCKEYKKGDKL------LRHWASEIEHGQRNAQKCPK 442

Query: 488 CNHMIELAEGCYHMTC-RCGHEFCYKCGAEWK 510
           C   I+  EGC HMTC +C   FCY+CG  ++
Sbjct: 443 CKIHIQRTEGCDHMTCSQCNTNFCYRCGERYR 457

BLAST of MELO3C025743.1 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 85.1 bits (209), Expect = 2.7e-15
Identity = 61/212 (28.77%), Postives = 91/212 (42.92%), Query Frame = 0

Query: 308 CVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVP-KCPHDGCKFDLNVDSCA 367
           C +CLED  +     +  C    C  C+K ++  ++  G V  KCP   C   L   +  
Sbjct: 236 CRVCLEDKPIK---PLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLEETTVV 295

Query: 368 KFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVR- 427
             LT +D    +  ++   I  S K   P P+C    T  +     K      ++S  R 
Sbjct: 296 YNLTHEDSIKYKYFLELGRIDSSTK---PCPQCKHFTTFKK-----KGHIPTPSRSESRY 355

Query: 428 --KCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQ-----CVK 487
             +C  C  ++C  C  PWH  + C +YK+ + L      L+  AS     Q     C K
Sbjct: 356 KIQCPTCQLIWCFKCHSPWHEGVNCKEYKKGDKL------LRHWASEIEHGQRNAQKCPK 415

Query: 488 CNHMIELAEGCYHMTC-RCGHEFCYKCGAEWK 510
           C   I+  EGC HMTC +C   FCY+CG  ++
Sbjct: 416 CKIHIQRTEGCDHMTCSQCNTNFCYRCGERYR 430

BLAST of MELO3C025743.1 vs. NCBI nr
Match: XP_008463909.1 (PREDICTED: uncharacterized protein LOC103501921 [Cucumis melo])

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0

Query: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60
           MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI
Sbjct: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60

Query: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120
           PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE
Sbjct: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120

Query: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180
           ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA
Sbjct: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180

Query: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240
           ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT
Sbjct: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240

Query: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300
           GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP
Sbjct: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300

Query: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360
           KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL
Sbjct: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360

Query: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420
           NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN
Sbjct: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420

Query: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480
           QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN
Sbjct: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480

Query: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540
           HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ
Sbjct: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540

Query: 541 YYDDEDEEELYDSEDEYFILFG 563
           YYDDEDEEELYDSEDEYFILFG
Sbjct: 541 YYDDEDEEELYDSEDEYFILFG 562

BLAST of MELO3C025743.1 vs. NCBI nr
Match: XP_004149554.1 (uncharacterized protein LOC101214830 [Cucumis sativus] >KGN47299.1 hypothetical protein Csa_022983 [Cucumis sativus])

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 523/563 (92.90%), Postives = 540/563 (95.91%), Query Frame = 0

Query: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60
           MAAKPSLDSDD+DF AI+S+QLRDFMDVLSMESDL+LAYNLQLEEALVASLASSSSSSSI
Sbjct: 1   MAAKPSLDSDDDDFSAISSQQLRDFMDVLSMESDLDLAYNLQLEEALVASLASSSSSSSI 60

Query: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120
            RPEVQ+FE V  PRIGTLHSRD+E+CDRIFQDWLQTEFDMRRTGGERHRQ  NHG AR 
Sbjct: 61  HRPEVQNFEHVDRPRIGTLHSRDVEECDRIFQDWLQTEFDMRRTGGERHRQVHNHGCARG 120

Query: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180
           ILNI DD+WRDQS+ SRKPFGEGCSNGV+D+ VFKLYFKGLV EEEIGNEKRVVAGIGVA
Sbjct: 121 ILNIRDDDWRDQSENSRKPFGEGCSNGVDDQGVFKLYFKGLVGEEEIGNEKRVVAGIGVA 180

Query: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240
           ICNPEDKLVVEVKRRL GNERSKIVAELKALIAG NVA+DLKLKRLCFY DYYPLFQFIT
Sbjct: 181 ICNPEDKLVVEVKRRLPGNERSKIVAELKALIAGFNVALDLKLKRLCFYSDYYPLFQFIT 240

Query: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300
           GRWPPKQRKVAALLSQLAHLRVRFDSC+HVHVAR+DIKYAFKLARDAIGPQVTQTEVPAP
Sbjct: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCTHVHVARHDIKYAFKLARDAIGPQVTQTEVPAP 300

Query: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360
           KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL
Sbjct: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360

Query: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420
           NVDSCAKFLTPKDMAT+RQRIKEASIPVSEKVYCPYPRCSALM KVEVL YTKDVF  AN
Sbjct: 361 NVDSCAKFLTPKDMATMRQRIKEASIPVSEKVYCPYPRCSALMRKVEVLAYTKDVFGTAN 420

Query: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480
           QSGVRKCMKCHGLFCI+CKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN
Sbjct: 421 QSGVRKCMKCHGLFCIDCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480

Query: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540
           HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWH DDDDRD DHDD+
Sbjct: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWH-DDDDRDFDHDDR 540

Query: 541 YYDD-EDEEELYDSEDEYFILFG 563
           YYDD EDEEELYDSEDEYFILFG
Sbjct: 541 YYDDEEDEEELYDSEDEYFILFG 562

BLAST of MELO3C025743.1 vs. NCBI nr
Match: XP_038902640.1 (uncharacterized protein LOC120089288 [Benincasa hispida])

HSP 1 Score: 999.6 bits (2583), Expect = 1.1e-287
Identity = 483/559 (86.40%), Postives = 515/559 (92.13%), Query Frame = 0

Query: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60
           MAAKPSL+SD ED  AI S+   D MD L++ESDL+LAYNLQLEEALVASLASSSSSSSI
Sbjct: 9   MAAKPSLNSDGEDLNAIVSEHFCDLMDALALESDLDLAYNLQLEEALVASLASSSSSSSI 68

Query: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120
           PRPE QDFERV I RIGTLHSRDI K D I QDWLQ+EFDMRRTG E H Q  NHGFAR+
Sbjct: 69  PRPEFQDFERVDISRIGTLHSRDIAKYDEILQDWLQSEFDMRRTGDELHPQVHNHGFARD 128

Query: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180
           ILNI DD+WR Q DTSRKPFGEGCSNGVE+R VFKLYFKGLV EE+IGNE+R VAGIGVA
Sbjct: 129 ILNIPDDDWRVQGDTSRKPFGEGCSNGVENRGVFKLYFKGLVIEEDIGNERRFVAGIGVA 188

Query: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240
           ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGL+VAMDLKLKRLCFYCDYYPLFQF+T
Sbjct: 189 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLDVAMDLKLKRLCFYCDYYPLFQFVT 248

Query: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300
           G+WPPKQRK+AALLSQ+AHLRVRFDSC  VHVAR+DIKYAFKLARDAI PQ+ QTE PAP
Sbjct: 249 GKWPPKQRKIAALLSQVAHLRVRFDSCYPVHVARHDIKYAFKLARDAIVPQIIQTEAPAP 308

Query: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360
           KKKLNETCVICLEDCDV+RMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL
Sbjct: 309 KKKLNETCVICLEDCDVNRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 368

Query: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420
           NVDSCAKFLTPKDMAT+RQRIKEASIPV+EKVYCPYPRCS LMTKVEVL YTK++  A N
Sbjct: 369 NVDSCAKFLTPKDMATMRQRIKEASIPVAEKVYCPYPRCSTLMTKVEVLAYTKEILGAVN 428

Query: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480
           QSGVRKCMKCHGLFCI+CKVPWHN ITCNDYKRSNNLPTEDVKLKSLAST LWRQCVKCN
Sbjct: 429 QSGVRKCMKCHGLFCIDCKVPWHNHITCNDYKRSNNLPTEDVKLKSLASTYLWRQCVKCN 488

Query: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540
           HMIELAEGC+HMTCRCGHEFCYKCGAEWK+KKATCSCPLW E+ IW+ +DDDRD DHDDQ
Sbjct: 489 HMIELAEGCHHMTCRCGHEFCYKCGAEWKDKKATCSCPLWTEDRIWY-NDDDRDFDHDDQ 548

Query: 541 YYDDED-EEELYDSEDEYF 559
           YYD+ED EEELYDSEDE++
Sbjct: 549 YYDEEDGEEELYDSEDEFY 566

BLAST of MELO3C025743.1 vs. NCBI nr
Match: XP_023511953.1 (uncharacterized protein LOC111776808 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 875.5 bits (2261), Expect = 2.4e-250
Identity = 423/540 (78.33%), Postives = 478/540 (88.52%), Query Frame = 0

Query: 24  DFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSIPRPEVQDFERVGIPRIGTLHSRD 83
           D MD L  ESDL+LAYNLQLEEAL ASLASSSSS+SIPRP  Q+FER  I R  ++HSRD
Sbjct: 9   DPMDALGFESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDISRTASIHSRD 68

Query: 84  IEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFAREILNIHDDNWRDQSDTSRKP-FGE 143
           IE  D+IFQDWLQ+EF+MRRT GE  R+ +NHGFAREILNI DD+  +  DTS+   FGE
Sbjct: 69  IENDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFAREILNISDDDCDEWGDTSQNHFFGE 128

Query: 144 GCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLAGNERS 203
           GCS GVE++ VFKLYFKGLVSEE IG E+R+VAGIGVAICNPED+LV EVK+ L GNERS
Sbjct: 129 GCSKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLVGNERS 188

Query: 204 KIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHL-R 263
           KIVAE KALI GL+ AMDLKLKRLCFYCDYYPL+QF++G+WPPKQRK+ ALL ++ HL +
Sbjct: 189 KIVAEFKALITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQ 248

Query: 264 VRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMF 323
             FDSC    VAR+DIK+AFKLAR+AI PQ+T+   PAPKKKL+ETCVICLE+CD +RMF
Sbjct: 249 AHFDSCYPRLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCVICLEECDFNRMF 308

Query: 324 AVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRI 383
           AVDGCSHRYC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMAT+RQRI
Sbjct: 309 AVDGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATMRQRI 368

Query: 384 KEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVP 443
           KEASIPVSEKVYCPYPRCSALMTKVEVL YT+D+FS ANQSG+RKCMKCHGLFCINCKVP
Sbjct: 369 KEASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCINCKVP 428

Query: 444 WHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFC 503
           WHNR++CNDYKRSNNLPT+DVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFC
Sbjct: 429 WHNRVSCNDYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFC 488

Query: 504 YKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDEDEE-ELYDSEDEYFIL 561
           YKCG EWK+KKATCSCPLWAENHI + +DD+R+  HD ++YD+EDEE ELYDS+DE++ +
Sbjct: 489 YKCGGEWKDKKATCSCPLWAENHILY-NDDNREFHHDGRFYDEEDEEDELYDSDDEFYYI 547

BLAST of MELO3C025743.1 vs. NCBI nr
Match: XP_022985536.1 (uncharacterized protein LOC111483577 [Cucurbita maxima])

HSP 1 Score: 873.2 bits (2255), Expect = 1.2e-249
Identity = 423/538 (78.62%), Postives = 475/538 (88.29%), Query Frame = 0

Query: 26  MDVLSMESDLELAYNLQLEEALVASLASSSSSSSIPRPEVQDFERVGIPRIGTLHSRDIE 85
           MD L +ESDL+LAYNLQLEEAL ASLASSSSS+S PRP  Q+FER  I R  TLHSRDIE
Sbjct: 1   MDDLVLESDLDLAYNLQLEEALAASLASSSSSASNPRPGFQEFERDDISRTATLHSRDIE 60

Query: 86  KCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFAREILNIHDDNWRDQSDTSRKP-FGEGC 145
             D+IFQDWLQ+EF+MRRT GE  R+ +NHGFAREILNI DD+  +  DTS+   FGEGC
Sbjct: 61  NDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFAREILNISDDDCDEWGDTSQNHFFGEGC 120

Query: 146 SNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLAGNERSKI 205
           S GVE++ VFKLYFKGLVSEE IG E+R+VAGIGVAICNPED+LV EVK+ LAGNERSKI
Sbjct: 121 SKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLAGNERSKI 180

Query: 206 VAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHL-RVR 265
           VAE KALI GL+ AMDL LKRLCFYCDYYPL+QF++G+WPPKQRK+  LL ++ HL +  
Sbjct: 181 VAEFKALITGLDAAMDLNLKRLCFYCDYYPLYQFVSGKWPPKQRKIVTLLDRVTHLQQAH 240

Query: 266 FDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAV 325
           FDSC    VAR+DIK+AFKLAR+AI PQ T+   PAPKKKL+ETCVICLE+CD +RMFAV
Sbjct: 241 FDSCYPRLVARHDIKFAFKLAREAIVPQTTRPVDPAPKKKLHETCVICLEECDFNRMFAV 300

Query: 326 DGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKE 385
           DGCSHRYC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM T+RQRIKE
Sbjct: 301 DGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMTTMRQRIKE 360

Query: 386 ASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWH 445
           ASIPVSEKVYCPYPRCSALMTKVEVL YT+D+FS ANQSG+RKCMKCHGLFCINCKVPWH
Sbjct: 361 ASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCINCKVPWH 420

Query: 446 NRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYK 505
           NR++CNDYKRSNNLPT+DVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYK
Sbjct: 421 NRVSCNDYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYK 480

Query: 506 CGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDED-EEELYDSEDEYFIL 561
           CG EWK+KKATCSCPLWAENHI + +DD+R+ DHD Q+YD+ED EEELYDS+DE++ +
Sbjct: 481 CGGEWKDKKATCSCPLWAENHILY-NDDNREFDHDGQFYDEEDEEEELYDSDDEFYYI 537

BLAST of MELO3C025743.1 vs. ExPASy TrEMBL
Match: A0A1S3CK99 (uncharacterized protein LOC103501921 OS=Cucumis melo OX=3656 GN=LOC103501921 PE=3 SV=1)

HSP 1 Score: 1165.6 bits (3014), Expect = 0.0e+00
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0

Query: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60
           MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI
Sbjct: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60

Query: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120
           PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE
Sbjct: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120

Query: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180
           ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA
Sbjct: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180

Query: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240
           ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT
Sbjct: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240

Query: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300
           GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP
Sbjct: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300

Query: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360
           KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL
Sbjct: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360

Query: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420
           NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN
Sbjct: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420

Query: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480
           QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN
Sbjct: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480

Query: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540
           HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ
Sbjct: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540

Query: 541 YYDDEDEEELYDSEDEYFILFG 563
           YYDDEDEEELYDSEDEYFILFG
Sbjct: 541 YYDDEDEEELYDSEDEYFILFG 562

BLAST of MELO3C025743.1 vs. ExPASy TrEMBL
Match: A0A0A0KEP0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290800 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 523/563 (92.90%), Postives = 540/563 (95.91%), Query Frame = 0

Query: 1   MAAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLASSSSSSSI 60
           MAAKPSLDSDD+DF AI+S+QLRDFMDVLSMESDL+LAYNLQLEEALVASLASSSSSSSI
Sbjct: 1   MAAKPSLDSDDDDFSAISSQQLRDFMDVLSMESDLDLAYNLQLEEALVASLASSSSSSSI 60

Query: 61  PRPEVQDFERVGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFARE 120
            RPEVQ+FE V  PRIGTLHSRD+E+CDRIFQDWLQTEFDMRRTGGERHRQ  NHG AR 
Sbjct: 61  HRPEVQNFEHVDRPRIGTLHSRDVEECDRIFQDWLQTEFDMRRTGGERHRQVHNHGCARG 120

Query: 121 ILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVA 180
           ILNI DD+WRDQS+ SRKPFGEGCSNGV+D+ VFKLYFKGLV EEEIGNEKRVVAGIGVA
Sbjct: 121 ILNIRDDDWRDQSENSRKPFGEGCSNGVDDQGVFKLYFKGLVGEEEIGNEKRVVAGIGVA 180

Query: 181 ICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFIT 240
           ICNPEDKLVVEVKRRL GNERSKIVAELKALIAG NVA+DLKLKRLCFY DYYPLFQFIT
Sbjct: 181 ICNPEDKLVVEVKRRLPGNERSKIVAELKALIAGFNVALDLKLKRLCFYSDYYPLFQFIT 240

Query: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAP 300
           GRWPPKQRKVAALLSQLAHLRVRFDSC+HVHVAR+DIKYAFKLARDAIGPQVTQTEVPAP
Sbjct: 241 GRWPPKQRKVAALLSQLAHLRVRFDSCTHVHVARHDIKYAFKLARDAIGPQVTQTEVPAP 300

Query: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360
           KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL
Sbjct: 301 KKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 360

Query: 361 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 420
           NVDSCAKFLTPKDMAT+RQRIKEASIPVSEKVYCPYPRCSALM KVEVL YTKDVF  AN
Sbjct: 361 NVDSCAKFLTPKDMATMRQRIKEASIPVSEKVYCPYPRCSALMRKVEVLAYTKDVFGTAN 420

Query: 421 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480
           QSGVRKCMKCHGLFCI+CKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN
Sbjct: 421 QSGVRKCMKCHGLFCIDCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCN 480

Query: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQ 540
           HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWH DDDDRD DHDD+
Sbjct: 481 HMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWH-DDDDRDFDHDDR 540

Query: 541 YYDD-EDEEELYDSEDEYFILFG 563
           YYDD EDEEELYDSEDEYFILFG
Sbjct: 541 YYDDEEDEEELYDSEDEYFILFG 562

BLAST of MELO3C025743.1 vs. ExPASy TrEMBL
Match: A0A6J1J8H4 (uncharacterized protein LOC111483577 OS=Cucurbita maxima OX=3661 GN=LOC111483577 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.7e-250
Identity = 423/538 (78.62%), Postives = 475/538 (88.29%), Query Frame = 0

Query: 26  MDVLSMESDLELAYNLQLEEALVASLASSSSSSSIPRPEVQDFERVGIPRIGTLHSRDIE 85
           MD L +ESDL+LAYNLQLEEAL ASLASSSSS+S PRP  Q+FER  I R  TLHSRDIE
Sbjct: 1   MDDLVLESDLDLAYNLQLEEALAASLASSSSSASNPRPGFQEFERDDISRTATLHSRDIE 60

Query: 86  KCDRIFQDWLQTEFDMRRTGGERHRQGQNHGFAREILNIHDDNWRDQSDTSRKP-FGEGC 145
             D+IFQDWLQ+EF+MRRT GE  R+ +NHGFAREILNI DD+  +  DTS+   FGEGC
Sbjct: 61  NDDQIFQDWLQSEFEMRRTVGEVDRRVRNHGFAREILNISDDDCDEWGDTSQNHFFGEGC 120

Query: 146 SNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLAGNERSKI 205
           S GVE++ VFKLYFKGLVSEE IG E+R+VAGIGVAICNPED+LV EVK+ LAGNERSKI
Sbjct: 121 SKGVENQGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEDELVFEVKKHLAGNERSKI 180

Query: 206 VAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHL-RVR 265
           VAE KALI GL+ AMDL LKRLCFYCDYYPL+QF++G+WPPKQRK+  LL ++ HL +  
Sbjct: 181 VAEFKALITGLDAAMDLNLKRLCFYCDYYPLYQFVSGKWPPKQRKIVTLLDRVTHLQQAH 240

Query: 266 FDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAV 325
           FDSC    VAR+DIK+AFKLAR+AI PQ T+   PAPKKKL+ETCVICLE+CD +RMFAV
Sbjct: 241 FDSCYPRLVARHDIKFAFKLAREAIVPQTTRPVDPAPKKKLHETCVICLEECDFNRMFAV 300

Query: 326 DGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKE 385
           DGCSHRYC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM T+RQRIKE
Sbjct: 301 DGCSHRYCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMTTMRQRIKE 360

Query: 386 ASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWH 445
           ASIPVSEKVYCPYPRCSALMTKVEVL YT+D+FS ANQSG+RKCMKCHGLFCINCKVPWH
Sbjct: 361 ASIPVSEKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCINCKVPWH 420

Query: 446 NRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYK 505
           NR++CNDYKRSNNLPT+DVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYK
Sbjct: 421 NRVSCNDYKRSNNLPTDDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYK 480

Query: 506 CGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDED-EEELYDSEDEYFIL 561
           CG EWK+KKATCSCPLWAENHI + +DD+R+ DHD Q+YD+ED EEELYDS+DE++ +
Sbjct: 481 CGGEWKDKKATCSCPLWAENHILY-NDDNREFDHDGQFYDEEDEEEELYDSDDEFYYI 537

BLAST of MELO3C025743.1 vs. ExPASy TrEMBL
Match: A0A6J1FUK7 (uncharacterized protein LOC111448273 OS=Cucurbita moschata OX=3662 GN=LOC111448273 PE=3 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 5.0e-246
Identity = 413/532 (77.63%), Postives = 470/532 (88.35%), Query Frame = 0

Query: 32  ESDLELAYNLQLEEALVASLASSSSSSSIPRPEVQDFERVGIPRIGTLHSRDIEKCDRIF 91
           ESDL+LAYNLQLEEAL ASLASSSSS+SIPRP  Q+FER  I R  TLHSRDIE  D+IF
Sbjct: 11  ESDLDLAYNLQLEEALAASLASSSSSASIPRPGFQEFERDDITRTATLHSRDIENDDQIF 70

Query: 92  QDWLQTEFDMRRTGGERHRQGQNHGFAREILNIHDDNWRDQSDTSRKP-FGEGCSNGVED 151
           QDWLQ+EF+MRRT GE  R+ +NHGF REILNI DD+  +  DTS+   FGEGCS GVE+
Sbjct: 71  QDWLQSEFEMRRTVGEVDRRVRNHGFEREILNISDDDCDEWGDTSQNHFFGEGCSKGVEN 130

Query: 152 REVFKLYFKGLVSEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLAGNERSKIVAELKA 211
           + VFKLYFKGLVSEE IG E+R+VAGIGVAICNPE +LV EVK+ L GNERSKIVAE KA
Sbjct: 131 QGVFKLYFKGLVSEEGIGIERRLVAGIGVAICNPEGELVFEVKKHLVGNERSKIVAEFKA 190

Query: 212 LIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHL-RVRFDSCSH 271
           LI GL+ AMDLKLKRLCFYCDYYPL+QF++G+WPPKQRK+ ALL ++ HL +  FDSC  
Sbjct: 191 LITGLDAAMDLKLKRLCFYCDYYPLYQFVSGKWPPKQRKIVALLDRVTHLQQAHFDSCYP 250

Query: 272 VHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAVDGCSHR 331
             VAR+DIK+AFKLAR+AI PQ+T+   PAPKKKL+ETC+ICLE+CD +RMFAVDGCSHR
Sbjct: 251 RLVARHDIKFAFKLAREAIVPQITRPVDPAPKKKLHETCIICLEECDFNRMFAVDGCSHR 310

Query: 332 YCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKEASIPVS 391
           YC SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDM T+RQRIKEASIPVS
Sbjct: 311 YCSSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMVTMRQRIKEASIPVS 370

Query: 392 EKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWHNRITCN 451
           EKVYCPYPRCSALMTKVEVL YT+D+FS ANQSG+RKCMKCHGLFC+NCKVPWHNR++CN
Sbjct: 371 EKVYCPYPRCSALMTKVEVLEYTRDIFSGANQSGMRKCMKCHGLFCVNCKVPWHNRVSCN 430

Query: 452 DYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGAEWK 511
           DYKRSNNLPT+DVKL  LASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCG EWK
Sbjct: 431 DYKRSNNLPTDDVKLNYLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGGEWK 490

Query: 512 NKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDED-EEELYDSEDEYFIL 561
           +KKATCSCPLWAENHI + +DD+R+ +HD ++Y++ED EEELYDS+DE++ +
Sbjct: 491 DKKATCSCPLWAENHILY-NDDNREFNHDGRFYEEEDEEEELYDSDDEFYYI 541

BLAST of MELO3C025743.1 vs. ExPASy TrEMBL
Match: A0A6J1CGV8 (uncharacterized protein LOC111011310 OS=Momordica charantia OX=3673 GN=LOC111011310 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 7.2e-245
Identity = 421/563 (74.78%), Postives = 478/563 (84.90%), Query Frame = 0

Query: 2   AAKPSLDSDDEDFGAIASKQLRDFMDVLSMESDLELAYNLQLEEALVASLAS---SSSSS 61
           A  PS   D ED  AI  +Q R+ MD L+++SDL+ AY+LQLEEAL ASLAS   +SSSS
Sbjct: 5   ADPPSPFYDCEDLSAIKMEQFRELMDALALDSDLDFAYHLQLEEALAASLASQAPASSSS 64

Query: 62  SIPRPEVQDFER-VGIPRIGTLHSRDIEKCDRIFQDWLQTEFDMRRTGGERHRQGQNHGF 121
           SI R E+Q+FER      IG+LH++D+ K D+IFQDWLQ +F+MRRTGGE HR+  +HGF
Sbjct: 65  SILRSELQEFERDDDFSGIGSLHAQDVAKSDQIFQDWLQCQFEMRRTGGELHRRIHDHGF 124

Query: 122 AREILNIHDDNWRDQSDTSRKPFGEGCSNGVEDREVFKLYFKGLVSEEEIGNEKRVVAGI 181
           AREI NI DD+W +  DT +KPFGEG S GVE++    LYFKGLVSEE IGNE+R V GI
Sbjct: 125 AREIFNIRDDDWSECGDTFQKPFGEGSSKGVENQGFSSLYFKGLVSEEGIGNERRAVVGI 184

Query: 182 GVAICNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQ 241
           GVAIC+PEDKLV E+K+ L GNERSKIVAE+KALI GLN AM LKLKRL FYCDYYPL+Q
Sbjct: 185 GVAICDPEDKLVFEMKKPLIGNERSKIVAEVKALIEGLNAAMGLKLKRLRFYCDYYPLYQ 244

Query: 242 FITGRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEV 301
           F+TG+WPPKQRK+A LLSQ+ HL++ FDSC    VAR+DIK+AFKLARDAI  ++T+TE 
Sbjct: 245 FVTGKWPPKQRKIAVLLSQVTHLQICFDSCKPKLVARHDIKFAFKLARDAIVTEITRTEG 304

Query: 302 PAPKKKLNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCK 361
           PA KKKLNE CVICLEDCDV+RMFAVDGC HRYCFSCMKQHVEVKLLQGLVPKCPHDGCK
Sbjct: 305 PALKKKLNEACVICLEDCDVNRMFAVDGCLHRYCFSCMKQHVEVKLLQGLVPKCPHDGCK 364

Query: 362 FDLNVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFS 421
           FDLNVDSCAKFLTPKDMAT+RQRIKEASIPVSEKVYCPYP+CSALMTK EVL YT+DV  
Sbjct: 365 FDLNVDSCAKFLTPKDMATMRQRIKEASIPVSEKVYCPYPKCSALMTKDEVLEYTRDVLG 424

Query: 422 AANQSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCV 481
            ANQSGVRKCMKCHGLFCINCKVPWHNR++CNDYKRSNN  +EDVKLKSLAST LWRQCV
Sbjct: 425 GANQSGVRKCMKCHGLFCINCKVPWHNRVSCNDYKRSNNPASEDVKLKSLASTNLWRQCV 484

Query: 482 KCNHMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDH 541
           KCNHMIELAEGCYHMTCRCGHEFCYKCG EWK+KKATCSCPLWAE+ IW+ +D++RD D 
Sbjct: 485 KCNHMIELAEGCYHMTCRCGHEFCYKCGGEWKDKKATCSCPLWAEDRIWY-NDNNRDFD- 544

Query: 542 DDQYYDDEDEEELYDSEDE-YFI 560
           DD  Y DE+EEE YDS+DE Y+I
Sbjct: 545 DDDIYTDEEEEEFYDSDDECYYI 565

BLAST of MELO3C025743.1 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-124
Identity = 248/568 (43.66%), Postives = 336/568 (59.15%), Query Frame = 0

Query: 31  MESDLELAYNLQLEEALVASLASSS-SSSSIPRPEVQDFERVGIPRIGTLHSRDIEKCDR 90
           M+ D E A+ LQ+EEAL ASL+S S +    P P +       +   G L   +     +
Sbjct: 1   MDFDSEYAFRLQMEEALAASLSSQSRTPQRPPSPPI-------VATCGVLIVENNPNDSK 60

Query: 91  IFQDWLQTEFDMRRT--GGERHRQGQNH-----------------GFAREILNIHDDNWR 150
             +      FD +R   GG    +G+ H                 G +R     H +   
Sbjct: 61  TAKTSSGYGFDFKRAVGGGNSKAKGKTHESVTGVRTDERNPNIGLGNSRSTSTGHGNTKP 120

Query: 151 DQSDTSR--KPFGEGCS------NGVEDREVFKLYFKGLVSEEE-IGNEKRVVAGIGVAI 210
              +T R  +  GEG S        +    +++LYFKGLVS+E   G    VV+G GVAI
Sbjct: 121 LPQETVRPAQLVGEGSSRVNTAAGKINGNLLYRLYFKGLVSDENGKGKMTDVVSGFGVAI 180

Query: 211 CNPEDKLVVEVKRRLAGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFITG 270
           C+  D L+ E+K  L  N  S+  AELKALI GL  A+ L +K + F+CD YP+FQ++TG
Sbjct: 181 CDQRDNLLFEMKGPLIDNGMSRQGAELKALIRGLTEALKLGIKHIVFFCDSYPIFQYVTG 240

Query: 271 RWPPKQRKVAALLSQLAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAPK 330
           +W  KQ+K++ LL  L  +   F S  HV VARND+K+A+KLAR++I   VT  E P   
Sbjct: 241 KWMAKQKKISLLLDDLQSIMQHFSSYQHVLVARNDVKFAYKLARESILSMVTPHEDPRQA 300

Query: 331 KK-LNETCVICLEDCDVSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDL 390
           K  L E C IC  D     MF+VD C HR+CF C+KQHVEVKLL G+ PKCPHDGCK +L
Sbjct: 301 KAVLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDGCKSEL 360

Query: 391 NVDSCAKFLTPKDMATLRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAAN 450
            +D+C K LTPK     +QR++E +IPV+E+VYCPYPRCSALM+K ++    K + S   
Sbjct: 361 VIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSLLSLYP 420

Query: 451 QSGVRKCMKCHGLFCINCKVPWHNRITCNDYKRSN-NLPTEDVKLKSLASTCLWRQCVKC 510
           +SGVR+C++C GLFC++CKVPWH  ++C +YK+ +   P +DVKLKSLA+  +WRQC KC
Sbjct: 421 KSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPADDVKLKSLANNKMWRQCGKC 480

Query: 511 NHMIELAEGCYHMTCRCGHEFCYKCGAEWKNKKATC--SCPLWAENHIWHDDDDDRDSDH 563
            HMIEL++GC H+TCRCGHEFCY CG  W     TC   CP W E +I    D  R +  
Sbjct: 481 QHMIELSQGCNHITCRCGHEFCYNCGGGWNKIMGTCLNRCPTWNEEYITR-QDPARANVA 540

BLAST of MELO3C025743.1 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 6.3e-92
Identity = 173/376 (46.01%), Postives = 239/376 (63.56%), Query Frame = 0

Query: 141 GEGCSNGVE--DREVFKLYFKGLVSEEEIGN--EKRVVAGIGVAICNPEDKLVVEVKRRL 200
           GEG S   +  D  V++L FKGLV+ E + +  EK V AG GVAIC+ +D L+ E+K  L
Sbjct: 139 GEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIKESL 198

Query: 201 AGNERSKIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQ 260
              E S+   E+ AL+ GL+ + DL ++ +  YCD   ++Q I GR   K +K+  L+ +
Sbjct: 199 RDIEISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSK-KKIDHLVEE 258

Query: 261 LAHLRVRFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCD 320
           +  +  +      V VARND+K+AF+LAR+AIG             +  ETC I  E+ D
Sbjct: 259 VQGILEKMACIDAVLVARNDVKFAFRLAREAIGRNSVDVNA-----EQGETCGIFFEETD 318

Query: 321 VSRMFAVDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMAT 380
           V  MF  + C HR+CF C+KQHV+VKL  G  P C   GCKF L ++ C+K LT K +  
Sbjct: 319 VEHMFVTEKCLHRHCFPCVKQHVKVKLRSGTEPTCLEYGCKFKLTLERCSKVLTLKLIEM 378

Query: 381 LRQRIKEASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCI 440
            +Q++KE SIP +E++YCPYP CS LM+K E       + S A+ S VR C+KC GLFCI
Sbjct: 379 WKQKMKEDSIPAAERIYCPYPNCSMLMSKTE-------LSSEADLSNVRTCVKCCGLFCI 438

Query: 441 NCKVPWHNRITCNDYKRSNNLP-TEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCR 500
           +CKVP H  ++ +DYK+ +  P  +D+KLKSLA+  +WRQCVKC HMIEL+ GC HMTCR
Sbjct: 439 DCKVPSHTDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCR 498

Query: 501 CGHEFCYKCGAEWKNK 512
           CG+EFCY+CG EW+ +
Sbjct: 499 CGYEFCYECGIEWQKR 501

BLAST of MELO3C025743.1 vs. TAIR 10
Match: AT2G21420.1 (IBR domain containing protein )

HSP 1 Score: 314.3 bits (804), Expect = 2.0e-85
Identity = 177/417 (42.45%), Postives = 251/417 (60.19%), Query Frame = 0

Query: 145 SNGVEDREVFKLYFKGLVSEEEIGNEKRVV-AGIGVAICNPEDKLVVEVKRRLAGNE-RS 204
           SN   D  V++L+FKGLVS+E   + + +V AG G+AIC+  + L+  +K+ L G++  +
Sbjct: 41  SNIFADSLVYRLFFKGLVSDETTTDMEEIVKAGFGIAICDEANTLLYNMKKSLNGDDVIN 100

Query: 205 KIVAELKALIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHLRV 264
               E+KALI  LNV++ ++L+ +   C  Y +FQ +TGR  P Q+ +  L+ Q+ HLR 
Sbjct: 101 PEEVEIKALICVLNVSIQMELRNVMICCGDYQIFQILTGRGKP-QQNIVHLVEQVQHLRG 160

Query: 265 RFDSCSHVHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFA 324
           +  S   V V R D+     LA +AIG                ETC IC E+ D  RMF 
Sbjct: 161 KLSSTEVVLVPRADV---IILAIEAIG---------------GETCCICRENTDADRMFF 220

Query: 325 VDGCSHRYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIK 384
            + C HR CFSC+ +HV+  LL G+ P C H  C  +L  +SC+K LTP  +   +++I+
Sbjct: 221 TENCFHRQCFSCVNRHVQRMLLCGISPTCLHFPCNSELTFESCSKVLTPNLIEFWKRKIE 280

Query: 385 EASIPVSEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPW 444
           E  +P ++K+YCPY RCS LM+K  +   T       +QS VR C+KC  LFCI+CKVP 
Sbjct: 281 EDLVPAADKIYCPYRRCSMLMSKTALSRET-------DQSNVRACIKCCRLFCIDCKVPS 340

Query: 445 HNRITCNDYKRSNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCY 504
           H  ++C DYK+ N  P  DVKLKSLA+   WRQCV+C++++EL EGC H+TCRCG EFCY
Sbjct: 341 HAGLSCVDYKKLNPDPLYDVKLKSLANKKKWRQCVQCSNLVELFEGCNHITCRCGFEFCY 400

Query: 505 KCGAEWKNKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDEDEEELYDSEDEYFI 560
            CG EW  +   C  P   ++    DDDDD D D DD   DD+D+++  D ED+ +I
Sbjct: 401 VCGKEWNQR--GCLSPGCDDD---DDDDDDDDDDDDDDDDDDDDDDDDEDDEDDGYI 426

BLAST of MELO3C025743.1 vs. TAIR 10
Match: AT3G45580.1 (RING/U-box protein with C6HC-type zinc finger )

HSP 1 Score: 307.4 bits (786), Expect = 2.4e-83
Identity = 154/381 (40.42%), Postives = 235/381 (61.68%), Query Frame = 0

Query: 154 FKLYFKGLVSEEEIGNEKRVVAGIGVAICNPEDKLVVEVKRRLAGNERSKIVAELKALIA 213
           ++LYFKGLVSEE +     ++AG GVAIC+ +D L+ ++K ++  +  + +  E+ AL  
Sbjct: 28  YRLYFKGLVSEETV----ELLAGFGVAICDKDDNLLFQMKEQVHDSRVTVLEVEIMALKR 87

Query: 214 GLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHLRVRFDSCSHVHVA 273
           GL  A+ L +  +  Y D+Y +F+ +  +    +   A L+  + H+R R  S   V V 
Sbjct: 88  GLTEAVGLGIDNISIYSDHYRIFELVMEKSASAEENFALLMDNVQHIRQRLTSSFPVLVT 147

Query: 274 RNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLED-CDVSRMFAVDGCSHRYCF 333
           RN IK+ ++LA + I   V++  +  P    ++TC IC +D  +   MF+V  C H +C 
Sbjct: 148 RNQIKFVYELAMETI---VSEISIHIPDH--DKTCSICSDDNFEPELMFSVALCGHEFCV 207

Query: 334 SCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKEASIPVSEKV 393
            C+K+H+EV+LL G VP+C H  C+  L + +CA  LT K  A    RI+E SIPV E+V
Sbjct: 208 ECVKRHIEVRLLAGGVPRCLHYQCESKLTLANCANLLTSKLKAMWELRIEEESIPVEERV 267

Query: 394 YCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWHNRITCNDYK 453
           YCP PRCS+LM+  ++   T++  +      +R C+KC   FCINCK+PWH+ ++CNDYK
Sbjct: 268 YCPNPRCSSLMSVTKLSNSTREDVT------MRSCVKCGEPFCINCKLPWHSNLSCNDYK 327

Query: 454 RSNNLPT-EDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGAEWKNK 513
                PT +D+KLK+LA+  +WRQC  C ++IEL+EGC H+TCRCGH+FCYKCGA+W   
Sbjct: 328 SLGPNPTADDIKLKALANQKMWRQCENCKNVIELSEGCMHITCRCGHQFCYKCGAKWITG 387

Query: 514 KATC--SCPLWAENHIWHDDD 531
           +  C  S P +  +   H+++
Sbjct: 388 RVFCTHSRPPYTPSMTSHEEE 393

BLAST of MELO3C025743.1 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 5.4e-83
Identity = 173/413 (41.89%), Postives = 243/413 (58.84%), Query Frame = 0

Query: 150 DREVFKLYFKGLVSEEEIGNEKRVV-AGIGVAICNPEDKLVVEVKRRLAGNERSKIVAEL 209
           D  V+KLYFKGLVS E   ++K +V  G GVAIC+  D L   + + L     ++  A++
Sbjct: 32  DNVVYKLYFKGLVSGETATDKKAIVKVGFGVAICDEADNLFYGMNKSLYEAVINREEADI 91

Query: 210 KALIAGLNVAMDLKLKRLCFYCDYYPLFQFITGRWPPKQRKVAALLSQLAHLRVRFDSCS 269
            ALI GL  ++   +K +   CD   ++Q I GR  P+Q+ V  LL ++  LR R  S  
Sbjct: 92  LALITGLYESIHRGVKNVVICCDDRQIYQTIIGREKPQQKNV-HLLEEVQRLRGRLASTG 151

Query: 270 HVHVARNDIKYAFKLARDAIGPQVTQTEVPAPKKKLNETCVICLEDCDVSRMFAVDGCSH 329
            V VA  D  +A +LA DA+        V A ++K   TC IC +  D   M   D C H
Sbjct: 152 TVLVATRDDNFALRLAIDAL--------VKATQEK-PLTCSICSDKTDAEHMLLNDKCLH 211

Query: 330 RYCFSCMKQHVEVKLLQGLVPKCPHDGCKFDLNVDSCAKFLTPKDMATLRQRIKEASIPV 389
           R+CFSC+KQ V+VKL  G+VP C  DGCK +L ++SC+  LTPK +   +++++E  IP 
Sbjct: 212 RHCFSCVKQQVKVKLRSGIVPPCLEDGCKSELTLESCSMVLTPKLIEMWKRKMEEDLIPD 271

Query: 390 SEKVYCPYPRCSALMTKVEVLTYTKDVFSAANQSGVRKCMKCHGLFCINCKVPWHNRITC 449
           +EK+YCPY  CS LM+K E       +   A QS VR C+KC  LFCI+CKVPWH+ ++C
Sbjct: 272 AEKIYCPYRSCSMLMSKTE-------LSREAEQSNVRACIKCSELFCIDCKVPWHSDLSC 331

Query: 450 NDYKR-SNNLPTEDVKLKSLASTCLWRQCVKCNHMIELAEGCYHMTCRCGHEFCYKCGAE 509
            DYKR  +     D+ LK LA+  +WRQC +C HMIEL EGC H+TCRCG+EFCY+CG +
Sbjct: 332 ADYKRIHSERLVNDMMLKVLANDQMWRQCSECKHMIELTEGCNHITCRCGYEFCYRCGHK 391

Query: 510 W-----------KNKKATCSCPLWAENHIWHDDDDDRDSDHDDQYYDDEDEEE 550
           W            N++      L  +  + +DDDDD D   D+ Y +D+D+++
Sbjct: 392 WTKYHHYSCQLMDNREDEEDYNLHVDAEVNNDDDDDEDYVFDEDYEEDDDDDD 427