MELO3C015526.1 (mRNA) Melon (DHL92) v4

BLAST of MELO3C015526.1 vs. NCBI nr
Match: XP_008450936.1 (PREDICTED: subtilisin-like protease SBT1.8 [Cucumis melo] >KAA0055770.1 subtilisin-like protease SBT1.8 [Cucumis melo var. makuwa])

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of MELO3C015526.1 vs. NCBI nr
Match: TYK10021.1 (subtilisin-like protease SBT1.8 [Cucumis melo var. makuwa])

HSP 1 Score: 1510.0 bits (3908), Expect = 0.0e+00
Identity = 761/763 (99.74%), Postives = 761/763 (99.74%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           MDSMPRFLIPF LLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS
Sbjct: 1   MDSMPRFLIPF-LLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CEAGPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 764
           RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of MELO3C015526.1 vs. NCBI nr
Match: XP_031740137.1 (subtilisin-like protease SBT1.8 [Cucumis sativus] >KAE8653457.1 hypothetical protein Csa_007123 [Cucumis sativus])

HSP 1 Score: 1496.5 bits (3873), Expect = 0.0e+00
Identity = 752/765 (98.30%), Postives = 758/765 (99.08%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           MDSMPR LIPF  LLLLLSCVFINAKKTYIVHMKHHALPS+YLTHHDWYSA+LQSLSSSS
Sbjct: 1   MDSMPRLLIPF--LLLLLSCVFINAKKTYIVHMKHHALPSQYLTHHDWYSANLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           SSDSLLYTYTSSFHGFAAFLDS+EVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSQEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CEAGPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPREN+SARDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESARDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAI DGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAIMDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSL+PAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDY+AFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYVAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAP VVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPPVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 763

BLAST of MELO3C015526.1 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1446.4 bits (3743), Expect = 0.0e+00
Identity = 728/765 (95.16%), Postives = 743/765 (97.12%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           M SM R LI F  LLLLL CVF+NAKKTYIV MKH+ALPSEYLTHHDWYSA LQSLSSSS
Sbjct: 1   MGSMARLLIAF--LLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           +SDSLLYTYTS++HGFAA LDS E ELLRQSDSVLGVYED+VY LHTTRTPGFLGLDSDF
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGH TQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+G DFSPSL
Sbjct: 121 GLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMG+KAV LVYNKGSNTSSNMCLPGSL+PAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           RKRYTVTFVASRDAAQT RFGFGSI W+N QHQVRSPV+FAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763

BLAST of MELO3C015526.1 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 715/766 (93.34%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MDSMPRFLI-PFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60
           M SM R L+  FLLLLLLL CVF+NAK+TYIV MKHHALPS+YLTHHDWYSA LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+S RDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNK VALVYNKGSN+SSNMCLPGSL+PA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLLYDISTNDYI FLCSLDYGIDHVQAIVKRSNITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           ERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPV+FAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of MELO3C015526.1 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 545/755 (72.19%), Postives = 630/755 (83.44%), Query Frame = 0

Query: 11  FLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLYTYT 70
           FL LLL        AKKTYI+ + H   P  +LTHHDWY++ L      +S  SLLYTYT
Sbjct: 17  FLFLLL-----HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQL------NSESSLLYTYT 76

Query: 71  SSFHGFAAFLDSEEVE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHTTQ 130
           +SFHGF+A+LDS E + LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+       
Sbjct: 77  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------H 136

Query: 131 DLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLIGAR 190
           DL  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+G CE+G DF   LCNKKLIGAR
Sbjct: 137 DLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 196

Query: 191 SFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARGMA 250
           SFSKG+QMASGGG+  K RE+ S RD DGHGTHT++TAAGS V NAS LGYA G ARGMA
Sbjct: 197 SFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMA 256

Query: 251 PQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAM 310
            +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGAF+AM
Sbjct: 257 TRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAM 316

Query: 311 EKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQ 370
           E+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG 
Sbjct: 317 ERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGV 376

Query: 371 GMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGGIG 430
           GMG K + LVYNKG+++SSN+CLPGSLD ++VRGK+VVCDRG+NARVEKG VVRDAGG+G
Sbjct: 377 GMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLG 436

Query: 431 MILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPSP 490
           MI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+SDS PTA+L F GT+L+V+PSP
Sbjct: 437 MIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSP 496

Query: 491 VVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTSMS 550
           VVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGL+ D R+TQFNIMSGTSMS
Sbjct: 497 VVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMS 556

Query: 551 CPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHV 610
           CPHISGLA LLKAAHP+WSPSAIKSALMTTAY  DNTN+ L DAA    SNP+AHG+GHV
Sbjct: 557 CPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHV 616

Query: 611 DPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFS 670
           DP KALSPGL+YDIST +YI FLCSLDY +DH+ AIVKR ++ CS+KF+DPGQLNYPSFS
Sbjct: 617 DPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFS 676

Query: 671 VVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTFV 730
           V+FG KRVVRYTR VTNVGAA SVY V       V ++VKPSKL F  VGE+KRYTVTFV
Sbjct: 677 VLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFV 736

Query: 731 ASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTR 765
           + +  + T +  FGSI WSN QH+VRSPV+F+W R
Sbjct: 737 SKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of MELO3C015526.1 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 2.3e-235
Identity = 428/761 (56.24%), Postives = 540/761 (70.96%), Query Frame = 0

Query: 11  FLLLLLLLSCVFINAKK---TYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLY 70
           F LLL L  C   ++     TYIVHM    +PS +  H +WY +SL+S+S S+    LLY
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLY 70

Query: 71  TYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHT 130
           TY ++ HGF+  L  EE + L     V+ V  +  Y LHTTRTP FLGLD        HT
Sbjct: 71  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE-------HT 130

Query: 131 TQDLNQAS--HDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKL 190
                +A    DV++GVLDTG+WPESKS+ D G   IPS W+GGCEAG +F+ SLCN+KL
Sbjct: 131 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 190

Query: 191 IGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIA 250
           IGAR F++GY+  S  G   + +E++S RD DGHGTHT+STAAGS V  ASLLGYA G A
Sbjct: 191 IGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 250

Query: 251 RGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGA 310
           RGMAP+ARVA YK CW  GCF SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGA
Sbjct: 251 RGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGA 310

Query: 311 FAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSL 370
           FAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL
Sbjct: 311 FAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSL 370

Query: 371 YSGQGMGNKAVALVY--NKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVR 430
           + G+ + +K +  +Y  N  + T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+
Sbjct: 371 FKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVK 430

Query: 431 DAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTIL 490
            AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +D NPTA +S  GT++
Sbjct: 431 AAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVV 490

Query: 491 NVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIM 550
            V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL +D R+ +FNI+
Sbjct: 491 GVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 550

Query: 551 SGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWA 610
           SGTSMSCPH+SGLAALLK+ HP+WSP+AI+SALMTTAY        L D A G  S P+ 
Sbjct: 551 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 610

Query: 611 HGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCS-RKFADPGQ 670
           HGAGHV P  A +PGL+YD++T DY+ FLC+L+Y    +++ V R N TC   K      
Sbjct: 611 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYTCDPSKSYSVAD 670

Query: 671 LNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERK 730
           LNYPSF+V        +YTR VT+VG AG+     T+  + VK++V+P+ L F +  E+K
Sbjct: 671 LNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 730

Query: 731 RYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 764
            YTVTF         +   FGSI WS+ +H V SPV+ +WT
Sbjct: 731 SYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of MELO3C015526.1 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 6.4e-217
Identity = 398/778 (51.16%), Postives = 515/778 (66.20%), Query Frame = 0

Query: 10  PFLLLLLLLSCVFINA--------KKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSS 69
           PFL ++L ++ +F+ A        KKTY++HM   A+P  Y  H  WYS+ + S++   S
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 70  SDS------LLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLG 129
            +       +LYTY ++FHG AA L  EE E L + D V+ V  +T Y LHTTR+P FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 130 LDSDFG--LWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAG 189
           L+      +W    T       HDV++GVLDTGIWPES+SF+DTGM  +P+ WRG CE G
Sbjct: 130 LERQESERVWAERVT------DHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETG 189

Query: 190 PDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVP 249
             F    CN+K++GAR F +GY+ A+  G   +  E +S RD+DGHGTHTA+T AGS V 
Sbjct: 190 KRFLKRNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVK 249

Query: 250 NASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGS 309
            A+L G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG 
Sbjct: 250 GANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGV 309

Query: 310 APYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ 369
           + Y RD+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V+
Sbjct: 310 STYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVK 369

Query: 370 LGNGKRFTGVSLYSGQGM--GNKAVALVY---NKGSNTSSNMCLPGSLDPAVVRGKVVVC 429
           +G  + F GVSLY G+ +   NK   LVY   N  S   ++ CL G+LD   V GK+V+C
Sbjct: 370 IGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVIC 429

Query: 430 DRGINARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS 489
           DRG+  RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +
Sbjct: 430 DRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMT 489

Query: 490 DSNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGP 549
               TA L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P
Sbjct: 490 SKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAP 549

Query: 550 TGLENDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNS 609
           + L +D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN   
Sbjct: 550 SSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFK 609

Query: 610 SLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKR 669
            L DA+G   S+P+ HGAGH+DP +A  PGL+YDI   +Y  FLC+ D     ++   K 
Sbjct: 610 PLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKH 669

Query: 670 SNITCSRKFA-DPGQLNYPSFSVVFGSK---RVVRYTRIVTNVGAAGSVYDVATTAPSVV 729
           SN TC    A +PG LNYP+ S +F      + +   R VTNVG   S Y V+ +     
Sbjct: 670 SNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGA 729

Query: 730 KVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAW 763
            VTV+P  L FT   ++  YTVTF   R   +  R  FG +VW +  H+VRSPV   W
Sbjct: 730 SVTVQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MELO3C015526.1 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.9e-216
Identity = 403/767 (52.54%), Postives = 519/767 (67.67%), Query Frame = 0

Query: 7   FLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLL 66
           F+ P LL     S    +  ++YIVH++    PS + +H++W+ + L+SL SS    +LL
Sbjct: 11  FVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLL 70

Query: 67  YTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH 126
           Y+Y+ + HGF+A L   +   LR+  SV+ V  D    +HTT TP FLG   + GLW   
Sbjct: 71  YSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSN- 130

Query: 127 TTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLI 186
                +    DVI+GVLDTGIWPE  SF D+G+  IPS W+G CE GPDF  S CN+KLI
Sbjct: 131 -----SNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLI 190

Query: 187 GARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIAR 246
           GAR+F +GY     G      +E++S RD +GHGTHTASTAAGS V NASL  YARG A 
Sbjct: 191 GARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTAT 250

Query: 247 GMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP-YYRDTIAIG 306
           GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP Y+ D+IAIG
Sbjct: 251 GMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIG 310

Query: 307 AFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVS 366
           AF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    G+GK FTG S
Sbjct: 311 AFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTS 370

Query: 367 LYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRD 426
           LY+G+ + +  ++LVY+   +  S +C PG L+ ++V GK+V+CDRG NARVEKG  V+ 
Sbjct: 371 LYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKL 430

Query: 427 AGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILN 486
           AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++  +PTA +SF GT++ 
Sbjct: 431 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 490

Query: 487 VR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIM 546
              PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT L+ D R+ QFNI+
Sbjct: 491 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 550

Query: 547 SGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWA 606
           SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D A G  SN + 
Sbjct: 551 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 610

Query: 607 HGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNI--TC-SRKFADP 666
           HGAGHVDP+KAL+PGL+YDI   +Y+AFLC++ Y    +   ++   +   C + K    
Sbjct: 611 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 670

Query: 667 GQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYDVATTAPSVVKVTVKPSKLVFTKV 726
           G LNYPSFSVVF S   VV+Y R+V NVG+   +VY+V   +P+ V++ V PSKL F+K 
Sbjct: 671 GDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKE 730

Query: 727 GERKRYTVTF---VASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAW 763
                Y VTF   V            FGSI W++ +H V+SPV+  W
Sbjct: 731 KSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of MELO3C015526.1 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 4.9e-209
Identity = 394/772 (51.04%), Postives = 519/772 (67.23%), Query Frame = 0

Query: 11  FLLLLLLLSCVFINAKK----TYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLL 70
           +   LL LS    +A      TYIVH+ H A PS + TH  WY++SL SL  +SS  S++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL--TSSPPSII 65

Query: 71  YTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS--DFGLWE 130
           +TY + FHGF+A L S++   L     V+ V  + V +LHTTR+P FLGL S    GL E
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 GHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKK 190
                  +    D++IGV+DTG+WPE  SFDD G+  +P +W+G C A  DF  S CN+K
Sbjct: 126 E------SDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 185

Query: 191 LIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGI 250
           L+GAR F  GY+  +  G   +  E +S RD DGHGTHTAS +AG +V  AS LGYA G+
Sbjct: 186 LVGARFFCGGYEATN--GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGV 245

Query: 251 ARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIG 310
           A GMAP+AR+AAYK CW +GC+ SDILA  D A++DGVDV+SLS+GG   PYY D IAIG
Sbjct: 246 AAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIG 305

Query: 311 AFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVS 370
           AF A+++G+FVS SAGN GP   ++ NVAPW+ TVGAGT+DRDFPA V+LGNGK  +GVS
Sbjct: 306 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 365

Query: 371 LYSGQGMG-NKAVALVYN----KGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 430
           +Y G G+   +   LVY      G   SS++CL GSLDP +V+GK+V+CDRGIN+R  KG
Sbjct: 366 VYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKG 425

Query: 431 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYV------RSDSNPT 490
            +VR  GG+GMI+AN    GE LVAD H+LPA +VG   GD IR+Y+      RS  +PT
Sbjct: 426 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 485

Query: 491 AVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLEN 550
           A + F GT L +RP+PVVA+FS+RGPN  TP+ILKPDVI PG+NILAAW + IGP+G+ +
Sbjct: 486 ATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTS 545

Query: 551 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDA 610
           D R+T+FNI+SGTSM+CPH+SGLAALLKAAHP WSP+AI+SAL+TTAYT DN+   + D 
Sbjct: 546 DNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDE 605

Query: 611 AGGGFSNPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKR-SNIT 670
           + G  S+   +G+GHV P KA+ PGL+YDI++ DYI FLC+ +Y   ++  I +R ++  
Sbjct: 606 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 665

Query: 671 CSRKFADPGQLNYPSFSVVF----GSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTV 730
            +R+    G LNYPSFSVVF     SK    + R VTNVG + SVY++    P    VTV
Sbjct: 666 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 725

Query: 731 KPSKLVFTKVGERKRYTVTFVAS--RDAAQTTRFGFGSIVWSNDQHQVRSPV 759
           +P KL F +VG++  + V    +  + +   T    G IVWS+ +  V SP+
Sbjct: 726 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of MELO3C015526.1 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of MELO3C015526.1 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of MELO3C015526.1 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1510.0 bits (3908), Expect = 0.0e+00
Identity = 761/763 (99.74%), Postives = 761/763 (99.74%), Query Frame = 0

Query: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60
           MDSMPRFLIPF LLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS
Sbjct: 1   MDSMPRFLIPF-LLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120
           SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180
           GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CEAGPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
           IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540
           TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600
           NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660
           PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 764
           RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of MELO3C015526.1 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1428.7 bits (3697), Expect = 0.0e+00
Identity = 715/766 (93.34%), Postives = 740/766 (96.61%), Query Frame = 0

Query: 1   MDSMPRFLI-PFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60
           M SM R L+  FLLLLLLL CVF+NAK+TYIV MKHHALPS+YLTHHDWYSA LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+S RDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNK VALVYNKGSN+SSNMCLPGSL+PA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGLLYDISTNDYI FLCSLDYGIDHVQAIVKRSNITCS+KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           ERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPV+FAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of MELO3C015526.1 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 713/766 (93.08%), Postives = 739/766 (96.48%), Query Frame = 0

Query: 1   MDSMPRFLI-PFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSS 60
           M SM R L+  FLLLLLLL CVF+NAK+TYIV MKHHALPS+YLTHHDWYSA LQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  SSSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120
           ++SDSLLYTYTS++HGFAA LD +E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPS 180
           FGLWEGH TQDL+QASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRG CE+GPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK REN+S RDQDGHGTHTASTAAGSHV NASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
           TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNK VALVYNKGSN+SSNMCLPGSL+PA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS + PTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQ 540
           GT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFAD 660
           NPWAHGAGHVDPHKALSPGL+YDISTNDYI FLCSLDYGIDHVQAI KRSNITC +KFAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVVKVTVKPSKLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 ERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 766
           ERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPV+FAWT+L
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of MELO3C015526.1 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 545/755 (72.19%), Postives = 630/755 (83.44%), Query Frame = 0

Query: 11  FLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLYTYT 70
           FL LLL        AKKTYI+ + H   P  +LTHHDWY++ L      +S  SLLYTYT
Sbjct: 17  FLFLLL-----HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQL------NSESSLLYTYT 76

Query: 71  SSFHGFAAFLDSEEVE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHTTQ 130
           +SFHGF+A+LDS E + LL  S+S+L ++ED +Y LHTTRTP FLGL+S+FG+       
Sbjct: 77  TSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------H 136

Query: 131 DLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLIGAR 190
           DL  +S+ VIIGVLDTG+WPES+SFDDT MPEIPS+W+G CE+G DF   LCNKKLIGAR
Sbjct: 137 DLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGAR 196

Query: 191 SFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARGMA 250
           SFSKG+QMASGGG+  K RE+ S RD DGHGTHT++TAAGS V NAS LGYA G ARGMA
Sbjct: 197 SFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMA 256

Query: 251 PQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAM 310
            +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAPYYRDTIAIGAF+AM
Sbjct: 257 TRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAM 316

Query: 311 EKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQ 370
           E+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG 
Sbjct: 317 ERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGV 376

Query: 371 GMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGGIG 430
           GMG K + LVYNKG+++SSN+CLPGSLD ++VRGK+VVCDRG+NARVEKG VVRDAGG+G
Sbjct: 377 GMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLG 436

Query: 431 MILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPSP 490
           MI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+SDS PTA+L F GT+L+V+PSP
Sbjct: 437 MIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSP 496

Query: 491 VVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTSMS 550
           VVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGL+ D R+TQFNIMSGTSMS
Sbjct: 497 VVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMS 556

Query: 551 CPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHV 610
           CPHISGLA LLKAAHP+WSPSAIKSALMTTAY  DNTN+ L DAA    SNP+AHG+GHV
Sbjct: 557 CPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHV 616

Query: 611 DPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFS 670
           DP KALSPGL+YDIST +YI FLCSLDY +DH+ AIVKR ++ CS+KF+DPGQLNYPSFS
Sbjct: 617 DPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFS 676

Query: 671 VVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTFV 730
           V+FG KRVVRYTR VTNVGAA SVY V       V ++VKPSKL F  VGE+KRYTVTFV
Sbjct: 677 VLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFV 736

Query: 731 ASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTR 765
           + +  + T +  FGSI WSN QH+VRSPV+F+W R
Sbjct: 737 SKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of MELO3C015526.1 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 816.6 bits (2108), Expect = 1.7e-236
Identity = 428/761 (56.24%), Postives = 540/761 (70.96%), Query Frame = 0

Query: 11  FLLLLLLLSCVFINAKK---TYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLY 70
           F LLL L  C   ++     TYIVHM    +PS +  H +WY +SL+S+S S+    LLY
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA---ELLY 70

Query: 71  TYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHT 130
           TY ++ HGF+  L  EE + L     V+ V  +  Y LHTTRTP FLGLD        HT
Sbjct: 71  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDE-------HT 130

Query: 131 TQDLNQAS--HDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKL 190
                +A    DV++GVLDTG+WPESKS+ D G   IPS W+GGCEAG +F+ SLCN+KL
Sbjct: 131 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 190

Query: 191 IGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIA 250
           IGAR F++GY+  S  G   + +E++S RD DGHGTHT+STAAGS V  ASLLGYA G A
Sbjct: 191 IGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTA 250

Query: 251 RGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGA 310
           RGMAP+ARVA YK CW  GCF SDILA +D+AI+D V+VLS+SLGGG + YYRD +AIGA
Sbjct: 251 RGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGA 310

Query: 311 FAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSL 370
           FAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LGNGK FTGVSL
Sbjct: 311 FAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSL 370

Query: 371 YSGQGMGNKAVALVY--NKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVR 430
           + G+ + +K +  +Y  N  + T+ N+C+ G+L P  V+GK+V+CDRGINARV+KG VV+
Sbjct: 371 FKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVK 430

Query: 431 DAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTIL 490
            AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +D NPTA +S  GT++
Sbjct: 431 AAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVV 490

Query: 491 NVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIM 550
            V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL +D R+ +FNI+
Sbjct: 491 GVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNII 550

Query: 551 SGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWA 610
           SGTSMSCPH+SGLAALLK+ HP+WSP+AI+SALMTTAY        L D A G  S P+ 
Sbjct: 551 SGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFD 610

Query: 611 HGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCS-RKFADPGQ 670
           HGAGHV P  A +PGL+YD++T DY+ FLC+L+Y    +++ V R N TC   K      
Sbjct: 611 HGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYTCDPSKSYSVAD 670

Query: 671 LNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERK 730
           LNYPSF+V        +YTR VT+VG AG+     T+  + VK++V+P+ L F +  E+K
Sbjct: 671 LNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKK 730

Query: 731 RYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 764
            YTVTF         +   FGSI WS+ +H V SPV+ +WT
Sbjct: 731 SYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of MELO3C015526.1 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 755.4 bits (1949), Expect = 4.5e-218
Identity = 398/778 (51.16%), Postives = 515/778 (66.20%), Query Frame = 0

Query: 10  PFLLLLLLLSCVFINA--------KKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSS 69
           PFL ++L ++ +F+ A        KKTY++HM   A+P  Y  H  WYS+ + S++   S
Sbjct: 10  PFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKS 69

Query: 70  SDS------LLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLG 129
            +       +LYTY ++FHG AA L  EE E L + D V+ V  +T Y LHTTR+P FLG
Sbjct: 70  QEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLG 129

Query: 130 LDSDFG--LWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAG 189
           L+      +W    T       HDV++GVLDTGIWPES+SF+DTGM  +P+ WRG CE G
Sbjct: 130 LERQESERVWAERVT------DHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETG 189

Query: 190 PDFSPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVP 249
             F    CN+K++GAR F +GY+ A+  G   +  E +S RD+DGHGTHTA+T AGS V 
Sbjct: 190 KRFLKRNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVK 249

Query: 250 NASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGS 309
            A+L G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG 
Sbjct: 250 GANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGV 309

Query: 310 APYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ 369
           + Y RD+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V+
Sbjct: 310 STYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVK 369

Query: 370 LGNGKRFTGVSLYSGQGM--GNKAVALVY---NKGSNTSSNMCLPGSLDPAVVRGKVVVC 429
           +G  + F GVSLY G+ +   NK   LVY   N  S   ++ CL G+LD   V GK+V+C
Sbjct: 370 IGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVIC 429

Query: 430 DRGINARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS 489
           DRG+  RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +
Sbjct: 430 DRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMT 489

Query: 490 DSNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGP 549
               TA L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P
Sbjct: 490 SKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAP 549

Query: 550 TGLENDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNS 609
           + L +D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN   
Sbjct: 550 SSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFK 609

Query: 610 SLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKR 669
            L DA+G   S+P+ HGAGH+DP +A  PGL+YDI   +Y  FLC+ D     ++   K 
Sbjct: 610 PLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKH 669

Query: 670 SNITCSRKFA-DPGQLNYPSFSVVFGSK---RVVRYTRIVTNVGAAGSVYDVATTAPSVV 729
           SN TC    A +PG LNYP+ S +F      + +   R VTNVG   S Y V+ +     
Sbjct: 670 SNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGA 729

Query: 730 KVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAW 763
            VTV+P  L FT   ++  YTVTF   R   +  R  FG +VW +  H+VRSPV   W
Sbjct: 730 SVTVQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of MELO3C015526.1 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 753.8 bits (1945), Expect = 1.3e-217
Identity = 403/767 (52.54%), Postives = 519/767 (67.67%), Query Frame = 0

Query: 7   FLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLL 66
           F+ P LL     S    +  ++YIVH++    PS + +H++W+ + L+SL SS    +LL
Sbjct: 11  FVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLL 70

Query: 67  YTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH 126
           Y+Y+ + HGF+A L   +   LR+  SV+ V  D    +HTT TP FLG   + GLW   
Sbjct: 71  YSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSN- 130

Query: 127 TTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLI 186
                +    DVI+GVLDTGIWPE  SF D+G+  IPS W+G CE GPDF  S CN+KLI
Sbjct: 131 -----SNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLI 190

Query: 187 GARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIAR 246
           GAR+F +GY     G      +E++S RD +GHGTHTASTAAGS V NASL  YARG A 
Sbjct: 191 GARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTAT 250

Query: 247 GMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP-YYRDTIAIG 306
           GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP Y+ D+IAIG
Sbjct: 251 GMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIG 310

Query: 307 AFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVS 366
           AF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    G+GK FTG S
Sbjct: 311 AFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTS 370

Query: 367 LYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRD 426
           LY+G+ + +  ++LVY+   +  S +C PG L+ ++V GK+V+CDRG NARVEKG  V+ 
Sbjct: 371 LYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKL 430

Query: 427 AGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILN 486
           AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++  +PTA +SF GT++ 
Sbjct: 431 AGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIG 490

Query: 487 VR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIM 546
              PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT L+ D R+ QFNI+
Sbjct: 491 PSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNII 550

Query: 547 SGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWA 606
           SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D A G  SN + 
Sbjct: 551 SGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFI 610

Query: 607 HGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNI--TC-SRKFADP 666
           HGAGHVDP+KAL+PGL+YDI   +Y+AFLC++ Y    +   ++   +   C + K    
Sbjct: 611 HGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTA 670

Query: 667 GQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYDVATTAPSVVKVTVKPSKLVFTKV 726
           G LNYPSFSVVF S   VV+Y R+V NVG+   +VY+V   +P+ V++ V PSKL F+K 
Sbjct: 671 GDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKE 730

Query: 727 GERKRYTVTF---VASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAW 763
                Y VTF   V            FGSI W++ +H V+SPV+  W
Sbjct: 731 KSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of MELO3C015526.1 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 729.2 bits (1881), Expect = 3.5e-210
Identity = 394/772 (51.04%), Postives = 519/772 (67.23%), Query Frame = 0

Query: 11  FLLLLLLLSCVFINAKK----TYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLL 70
           +   LL LS    +A      TYIVH+ H A PS + TH  WY++SL SL  +SS  S++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL--TSSPPSII 65

Query: 71  YTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDS--DFGLWE 130
           +TY + FHGF+A L S++   L     V+ V  + V +LHTTR+P FLGL S    GL E
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 GHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKK 190
                  +    D++IGV+DTG+WPE  SFDD G+  +P +W+G C A  DF  S CN+K
Sbjct: 126 E------SDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 185

Query: 191 LIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGI 250
           L+GAR F  GY+  +  G   +  E +S RD DGHGTHTAS +AG +V  AS LGYA G+
Sbjct: 186 LVGARFFCGGYEATN--GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGV 245

Query: 251 ARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIG 310
           A GMAP+AR+AAYK CW +GC+ SDILA  D A++DGVDV+SLS+GG   PYY D IAIG
Sbjct: 246 AAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIG 305

Query: 311 AFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVS 370
           AF A+++G+FVS SAGN GP   ++ NVAPW+ TVGAGT+DRDFPA V+LGNGK  +GVS
Sbjct: 306 AFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVS 365

Query: 371 LYSGQGMG-NKAVALVYN----KGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKG 430
           +Y G G+   +   LVY      G   SS++CL GSLDP +V+GK+V+CDRGIN+R  KG
Sbjct: 366 VYGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKG 425

Query: 431 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYV------RSDSNPT 490
            +VR  GG+GMI+AN    GE LVAD H+LPA +VG   GD IR+Y+      RS  +PT
Sbjct: 426 EIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPT 485

Query: 491 AVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLEN 550
           A + F GT L +RP+PVVA+FS+RGPN  TP+ILKPDVI PG+NILAAW + IGP+G+ +
Sbjct: 486 ATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTS 545

Query: 551 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDA 610
           D R+T+FNI+SGTSM+CPH+SGLAALLKAAHP WSP+AI+SAL+TTAYT DN+   + D 
Sbjct: 546 DNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDE 605

Query: 611 AGGGFSNPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKR-SNIT 670
           + G  S+   +G+GHV P KA+ PGL+YDI++ DYI FLC+ +Y   ++  I +R ++  
Sbjct: 606 STGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCD 665

Query: 671 CSRKFADPGQLNYPSFSVVF----GSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTV 730
            +R+    G LNYPSFSVVF     SK    + R VTNVG + SVY++    P    VTV
Sbjct: 666 GARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTV 725

Query: 731 KPSKLVFTKVGERKRYTVTFVAS--RDAAQTTRFGFGSIVWSNDQHQVRSPV 759
           +P KL F +VG++  + V    +  + +   T    G IVWS+ +  V SP+
Sbjct: 726 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767