MC11g_new0055.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0055.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ribonuclease E
Location	MC11: 1560313 .. 1560909 (-)
Sequence length	597
RNA-Seq Expression	MC11g_new0055.1
Synteny	MC11g_new0055.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGGTGGTGGTGGAGAGTGGACCGGAGGCGGTGGTGAATGGACTGGAGGAGGCGGGGAGTGCACCGGAGGTGGTGGAGAGAAAACCGGTGGGGGTGGGGAGAAAACTGGTGGTGGAGGAGATTGGACAGGTGGTGGTGGGGAGTGCACCGATGGTGGTGGGGAGTAGACCGGTGGTGGTGGAGAGTGCACCGGTGGTTGTGGAGGAGATTGGATCGGTGGTGGTGGAGAGTGCACCGGTGGTTGTGGAGGAGATTGGACGGGTGTTGGTGGGGAGTAGACCGGTGGTGGAGGAGAGTGCACCGGTGGTGGTGGAGGAGATTGGACGGGTGGTGGTGGGGAGTGCACCGGTGGTGGTGGAGAGTGCACCGGTGGTGGTGTGGAGTAGATCGGTGGTGGTGGAGATTGCACGGGTGCGGGTGGTGGAGATTGCACGGGTGCGGGTGGTGGAGATTGAACCGGTGCTGGTGGTGGAGATTGGATCGGTGCTGGTGGTGGAGATTGGATCGGTGCGGGTGGGGGCTGTTCCGGCTTTGGTGGCATGGACGTCGATGTGGTCGGTGATGGTGGTGACGATCCTCCGCCGCATTTAG

mRNA sequence

ATGGAGAAGGTGGTGGTGGAGAGTGGACCGGAGGCGGTGGTGAATGGACTGGAGGAGGCGGGGAGTGCACCGGAGGTGGTGGAGAGAAAACCGGTGGGGGTGGGGAGAAAACTGGTGGTGGAGGAGATTGGACAGGTGGTGGTGGGGAGTGCACCGATGGTGGTGGGGAGTAGACCGGTGGTGGTGGAGAGTGCACCGGTGGTTGTGGAGGAGATTGGATCGGTGGTGGTGGAGAGTGCACCGGTGGTTGTGGAGGAGATTGGACGGGTGTTGGTGGGGAGTAGACCGGTGGTGGAGGAGAGTGCACCGGTGGTGGTGGAGGAGATTGGACGGGTGGTGGTGGGGAGTGCACCGGTGGTGGTGGAGAGTGCACCGGTGGTGGTGTGGAGTAGATCGGTGGTGGTGGAGATTGCACGGGTGCGGGTGGTGGAGATTGCACGGGTGCGGGTGGTGGAGATTGAACCGGTGCTGGTGGTGGAGATTGGATCGGTGCTGGTGGTGGAGATTGGATCGGTGCGGGTGGGGGCTGTTCCGGCTTTGGTGGCATGGACGTCGATGTGGTCGGTGATGGTGGTGACGATCCTCCGCCGCATTTAG

Coding sequence (CDS)

ATGGAGAAGGTGGTGGTGGAGAGTGGACCGGAGGCGGTGGTGAATGGACTGGAGGAGGCGGGGAGTGCACCGGAGGTGGTGGAGAGAAAACCGGTGGGGGTGGGGAGAAAACTGGTGGTGGAGGAGATTGGACAGGTGGTGGTGGGGAGTGCACCGATGGTGGTGGGGAGTAGACCGGTGGTGGTGGAGAGTGCACCGGTGGTTGTGGAGGAGATTGGATCGGTGGTGGTGGAGAGTGCACCGGTGGTTGTGGAGGAGATTGGACGGGTGTTGGTGGGGAGTAGACCGGTGGTGGAGGAGAGTGCACCGGTGGTGGTGGAGGAGATTGGACGGGTGGTGGTGGGGAGTGCACCGGTGGTGGTGGAGAGTGCACCGGTGGTGGTGTGGAGTAGATCGGTGGTGGTGGAGATTGCACGGGTGCGGGTGGTGGAGATTGCACGGGTGCGGGTGGTGGAGATTGAACCGGTGCTGGTGGTGGAGATTGGATCGGTGCTGGTGGTGGAGATTGGATCGGTGCGGGTGGGGGCTGTTCCGGCTTTGGTGGCATGGACGTCGATGTGGTCGGTGATGGTGGTGACGATCCTCCGCCGCATTTAG

Protein sequence

MEKVVVESGPEAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVESAPVVVEEIGSVVVESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVESAPVVVWSRSVVVEIARVRVVEIARVRVVEIEPVLVVEIGSVLVVEIGSVRVGAVPALVAWTSMWSVMVVTILRRI

Homology

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BLAST of MC11g_new0055.1 vs. NCBI nr
Match: KRA29761.1 (ribonuclease E [Nocardioides sp. Root614] >KRA88479.1 ribonuclease E [Nocardioides sp. Root682])

HSP 1 Score: 76.3 bits (186), Expect = 3.41e-12
Identity = 66/120 (55.00%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 17  LEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVESAPVVVEEIGSVV 76
           +E A +A  V   K V    K   +E  +     AP+VV   PVVVE APVVVEE   VV
Sbjct: 862 VETAAAAKPVPSPKDVAAMAKHA-DEAHEDATDEAPVVVDEAPVVVEEAPVVVEE-APVV 921

Query: 77  VESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVESAPVVVWSRSVVVE 136
           VE APVVVEE   V+V   PVV E APVVVEE   VVV  APVVVE APVVV    VVVE
Sbjct: 922 VEEAPVVVEET-PVVVEEAPVVVEEAPVVVEE-AAVVVEEAPVVVEEAPVVVEEAPVVVE 977

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BLAST of MC11g_new0055.1 vs. NCBI nr
Match: WP_082574109.1 (MULTISPECIES: Rne/Rng family ribonuclease [unclassified Nocardioides])

HSP 1 Score: 76.3 bits (186), Expect = 3.41e-12
Identity = 66/120 (55.00%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 17  LEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVESAPVVVEEIGSVV 76
           +E A +A  V   K V    K   +E  +     AP+VV   PVVVE APVVVEE   VV
Sbjct: 871 VETAAAAKPVPSPKDVAAMAKHA-DEAHEDATDEAPVVVDEAPVVVEEAPVVVEE-APVV 930

Query: 77  VESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVESAPVVVWSRSVVVE 136
           VE APVVVEE   V+V   PVV E APVVVEE   VVV  APVVVE APVVV    VVVE
Sbjct: 931 VEEAPVVVEET-PVVVEEAPVVVEEAPVVVEE-AAVVVEEAPVVVEEAPVVVEEAPVVVE 986

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BLAST of MC11g_new0055.1 vs. NCBI nr
Match: XP_042337853.1 (calphotin-like, partial [Plectropomus leopardus])

HSP 1 Score: 75.1 bits (183), Expect = 8.03e-12
Identity = 92/172 (53.49%), Postives = 101/172 (58.72%), Query Frame = 0


Query: 4   VVVESGPEAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRP--VV 63
           VVVE  P  VV   EEA     VVE  PV     +VVEE   VVV  AP+VV      VV
Sbjct: 396 VVVEEAPVVVV---EEAPVV--VVEEAPV-----VVVEEAPVVVVDEAPVVVVDEAPVVV 455

Query: 64  VESAPVVVEEIGSVVVESAPVVV-EEIGRVLVGSRPVVE-ESAPVVVEEIGRVVVGSAPV 123
           V+ APVVV E   VVV  APVVV EE   V+V   PVV  E AP VVEE   VVV  APV
Sbjct: 456 VDEAPVVVVEEAPVVVVEAPVVVVEEAPVVVVDEAPVVVVEEAPGVVEEAPVVVVDEAPV 515

Query: 124 VV-ESAPVVVWSRS--VVVEIARVRVVEIARVRVVEIEPVLVVEIGSVLVVE 168
           VV E APVVV   +  VVVE A V VV+ A V VVE  PV+VV+   V+VVE
Sbjct: 516 VVVEEAPVVVVDEAPVVVVEEAPVVVVDGAPVVVVEEAPVVVVDEAPVVVVE 557

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BLAST of MC11g_new0055.1 vs. NCBI nr
Match: OMJ15117.1 (Aggrecan core protein, partial [Smittium culicis])

HSP 1 Score: 68.2 bits (165), Expect = 1.78e-09
Identity = 82/184 (44.57%), Postives = 106/184 (57.61%), Query Frame = 0


Query: 4   VVVESGP--EAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVV 63
           VV E+ P  E  +  +EEA   P V E  PV      VVEE   VV  +AP+V  + PVV
Sbjct: 61  VVEEAAPVVEEAIPAVEEA--VPVVEEATPVVEEATPVVEEAAPVVEEAAPVVEEATPVV 120

Query: 64  VESAPVVVEEIGSVVVESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVV 123
            E+AP VVEE   VV E  PVV EE   V+  + PVVEE+APVV +E+  VV  +APVV 
Sbjct: 121 EEAAP-VVEEATPVVEEVIPVV-EEAAPVVEEAAPVVEEAAPVV-QEVSPVVEEAAPVVE 180

Query: 124 ESAPVVVWSRSVVVEIARVRVVEIARVRVVEIEPVL-----VVEIGSVLVVEIGSVRVGA 180
           E+APVV  +  V+ E A V V E++ V V E+ PV+     VVE  S +V E+  V   A
Sbjct: 181 EAAPVVEEAIPVIEEAAPV-VEEVSPV-VEEVSPVVEEVSPVVEEVSPVVEEVSPVVEEA 237

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BLAST of MC11g_new0055.1 vs. NCBI nr
Match: XP_029284456.1 (proteoglycan 4-like [Cottoperca gobio])

HSP 1 Score: 67.8 bits (164), Expect = 2.54e-09
Identity = 66/135 (48.89%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 4   VVVESGPEAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVE 63
           V VES P    +      SAP V E  PV V    VVEE     V SAP+V    P  VE
Sbjct: 149 VAVESAPVVKEHAPAPVESAPVVEEHAPVAVESAPVVEEHAPAPVESAPVVEEHAPAPVE 208

Query: 64  SAPVVVEEIGSVVVESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVES 123
           SAP VVE   S  VESAP+V EE     V S PVVEE APV VE    +V   AP  VES
Sbjct: 209 SAP-VVEAQASSPVESAPIV-EEHAPAPVESAPVVEEHAPVAVES-APIVEEHAPAPVES 268

Query: 124 APVVVWSRSVVVEIA 138
           APVV    S  VE A
Sbjct: 269 APVVEAQASAPVESA 280

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BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL
Match: A0A0Q8DRE5 (Ribonuclease E OS=Nocardioides sp. Root614 OX=1736571 GN=ASD81_22375 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.65e-12
Identity = 66/120 (55.00%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 17  LEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVESAPVVVEEIGSVV 76
           +E A +A  V   K V    K   +E  +     AP+VV   PVVVE APVVVEE   VV
Sbjct: 862 VETAAAAKPVPSPKDVAAMAKHA-DEAHEDATDEAPVVVDEAPVVVEEAPVVVEE-APVV 921

Query: 77  VESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVESAPVVVWSRSVVVE 136
           VE APVVVEE   V+V   PVV E APVVVEE   VVV  APVVVE APVVV    VVVE
Sbjct: 922 VEEAPVVVEET-PVVVEEAPVVVEEAPVVVEE-AAVVVEEAPVVVEEAPVVVEEAPVVVE 977

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BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL
Match: A0A0Q8HQW8 (Ribonuclease E OS=Nocardioides sp. Root682 OX=1736586 GN=ASD84_19240 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.65e-12
Identity = 66/120 (55.00%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 17  LEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVESAPVVVEEIGSVV 76
           +E A +A  V   K V    K   +E  +     AP+VV   PVVVE APVVVEE   VV
Sbjct: 862 VETAAAAKPVPSPKDVAAMAKHA-DEAHEDATDEAPVVVDEAPVVVEEAPVVVEE-APVV 921

Query: 77  VESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVESAPVVVWSRSVVVE 136
           VE APVVVEE   V+V   PVV E APVVVEE   VVV  APVVVE APVVV    VVVE
Sbjct: 922 VEEAPVVVEET-PVVVEEAPVVVEEAPVVVEE-AAVVVEEAPVVVEEAPVVVEEAPVVVE 977

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BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL
Match: A0A1R1XKD6 (Aggrecan core protein (Fragment) OS=Smittium culicis OX=133412 GN=AYI69_g8316 PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 8.60e-10
Identity = 82/184 (44.57%), Postives = 106/184 (57.61%), Query Frame = 0


Query: 4   VVVESGP--EAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVV 63
           VV E+ P  E  +  +EEA   P V E  PV      VVEE   VV  +AP+V  + PVV
Sbjct: 61  VVEEAAPVVEEAIPAVEEA--VPVVEEATPVVEEATPVVEEAAPVVEEAAPVVEEATPVV 120

Query: 64  VESAPVVVEEIGSVVVESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVV 123
            E+AP VVEE   VV E  PVV EE   V+  + PVVEE+APVV +E+  VV  +APVV 
Sbjct: 121 EEAAP-VVEEATPVVEEVIPVV-EEAAPVVEEAAPVVEEAAPVV-QEVSPVVEEAAPVVE 180

Query: 124 ESAPVVVWSRSVVVEIARVRVVEIARVRVVEIEPVL-----VVEIGSVLVVEIGSVRVGA 180
           E+APVV  +  V+ E A V V E++ V V E+ PV+     VVE  S +V E+  V   A
Sbjct: 181 EAAPVVEEAIPVIEEAAPV-VEEVSPV-VEEVSPVVEEVSPVVEEVSPVVEEVSPVVEEA 237

[Close]

BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL
Match: A0A6J2PGL8 (proteoglycan 4-like OS=Cottoperca gobio OX=56716 GN=LOC115006384 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.23e-09
Identity = 66/135 (48.89%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 4   VVVESGPEAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVE 63
           V VES P    +      SAP V E  PV V    VVEE     V SAP+V    P  VE
Sbjct: 149 VAVESAPVVKEHAPAPVESAPVVEEHAPVAVESAPVVEEHAPAPVESAPVVEEHAPAPVE 208

Query: 64  SAPVVVEEIGSVVVESAPVVVEEIGRVLVGSRPVVEESAPVVVEEIGRVVVGSAPVVVES 123
           SAP VVE   S  VESAP+V EE     V S PVVEE APV VE    +V   AP  VES
Sbjct: 209 SAP-VVEAQASSPVESAPIV-EEHAPAPVESAPVVEEHAPVAVES-APIVEEHAPAPVES 268

Query: 124 APVVVWSRSVVVEIA 138
           APVV    S  VE A
Sbjct: 269 APVVEAQASAPVESA 280

[Close]

BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL
Match: A0A6J1DXA8 (uncharacterized protein LOC111024355 OS=Momordica charantia OX=3673 GN=LOC111024355 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.18e-09
Identity = 51/89 (57.30%), Postives = 57/89 (64.04%), Query Frame = 0


Query: 11  EAVVNGLEEAGSAPEVVERKPVGVGRKLVVEEIGQVVVGSAPMVVGSRPVVVESAPVVVE 70
           E   +GLE  GS P  VER+ V VG   VVE    VVVGS P+VV +R VVV SAP VVE
Sbjct: 47  EVAESGLEVVGSGPVGVERRRVAVGNVPVVEGSEPVVVGSEPVVVENRLVVVGSAP-VVE 106

Query: 71  EIGSVVVESAPVVVEEIGRVLVGSRPVVE 99
             G  VV +APVV E  G V+VGS PVVE
Sbjct: 107 VNGPEVVGNAPVV-EVNGLVVVGSAPVVE 133

The following BLAST results are available for this feature:

BLAST of MC11g_new0055.1 vs.


Match Name	E-value	Identity	Description

BLAST of MC11g_new0055.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KRA29761.1	3.41e-12	55.00	ribonuclease E [Nocardioides sp. Root614] >KRA88479.1 ribonuclease E [Nocardioid...	[more]
WP_082574109.1	3.41e-12	55.00	MULTISPECIES: Rne/Rng family ribonuclease [unclassified Nocardioides]	[more]
XP_042337853.1	8.03e-12	53.49	calphotin-like, partial [Plectropomus leopardus]	[more]
OMJ15117.1	1.78e-09	44.57	Aggrecan core protein, partial [Smittium culicis]	[more]
XP_029284456.1	2.54e-09	48.89	proteoglycan 4-like [Cottoperca gobio]	[more]

BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0Q8DRE5	1.65e-12	55.00	Ribonuclease E OS=Nocardioides sp. Root614 OX=1736571 GN=ASD81_22375 PE=4 SV=1	[more]
A0A0Q8HQW8	1.65e-12	55.00	Ribonuclease E OS=Nocardioides sp. Root682 OX=1736586 GN=ASD84_19240 PE=4 SV=1	[more]
A0A1R1XKD6	8.60e-10	44.57	Aggrecan core protein (Fragment) OS=Smittium culicis OX=133412 GN=AYI69_g8316 PE...	[more]
A0A6J2PGL8	1.23e-09	48.89	proteoglycan 4-like OS=Cottoperca gobio OX=56716 GN=LOC115006384 PE=4 SV=1	[more]
A0A6J1DXA8	3.18e-09	57.30	uncharacterized protein LOC111024355 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]

BLAST of MC11g_new0055.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g_new0055	MC11g_new0055	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g_new0055.1-cds	MC11g_new0055.1-cds-MC11:1560313..1560909	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g_new0055.1	MC11g_new0055.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0016757	glycosyltransferase activity
molecular_function	GO:0004518	nuclease activity
molecular_function	GO:0003676	nucleic acid binding