MC11g_new0033.1 (mRNA) Bitter gourd (Dali-11) v1

Overview


Name	MC11g_new0033.1
Type	mRNA
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cytochrome P450 CYP82D47-like
Location	MC11: 1415173 .. 1416263 (-)
Sequence length	474
RNA-Seq Expression	MC11g_new0033.1
Synteny	MC11g_new0033.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGAATCGGGAGAGGGGGGTGAAGATATCTGCAATTTTTTCTGATTGTCGATTGATTTTGAAATGGAACATGATGAATAATTAGTTTTGAAATGAAACCTTTGAATGTTGATAAAGTGATAATGATTTCTAAGTCATATATTTTCATAGCCATTGCTCACTTTACTAATCAAATTATATTATTGTTTGGGGAATGTAGGCTTTAATATTGGGTGGAGGTGATACTACAAGAGTAGGGATGATTTGGGCACTTTCATTGCTTCTCAACTATGAAAAAGTGTTGAAGAAGGCTCAACTTGAATTGGACGAACAAGTTGGGAGAGGAAGACTCGTGGAAGAGTCCGACATGAAAAATTTGTCATACCTCCGAGCGATAGTGAAGGAAGTATTGCGTTTGTACCCTCCAGCACCACTCTCAACCTTTCATGAGGCAATGGAAGATTGCACCGTTGATGGATACCACGTCCCTGCTGGGACACGCATGTTGGTGAATTTTCAGAAGCTCCAAAGAGACCCGCTTGTATGGGAAGAACCATATGAATTTCGGCCAAAGAGATTTTTGACTAGTCAAAAGGATTTTGATGTTAATGGACAGAGTCCACAATTGATACCATTTGGGAGTGGTCGAAGAATATGCCCTGGAGTCTCGTTTGCCAACCAAATTATGCTCCTTACACTTGCGAACCTACTTCGAAGTATTAAAAGAATATGAATTCATGGATTTGAAATTGACAAGCTGCTTGATGAACCAATCGACATGGAAGAGAGTTGTGGAATGACCAGTATTAAAAGAACTCTACTCGAAGTAGTTTTAACTCCGCGCCTTCCTGCCCAAATTTATGAATAAATTCAAGACAAAATTTATGGATAGGAAGAAAAAATATTTGCTCGCAAAAGTTTGAAAGAGTCCTCTAATTTGATTCTCAAATTTGTGTGTTAAAAATATTAGGGTGTATTGAGGAGTATTACTTTCTCAGCGTATGAATCAAAAAATTACTTCTTCCTCTGGAGTAGCTAAACAAAATGTGTTTAACAATAAAATAAGGTGGTATTTGTTCTAATATTTCAAATGTATTTTTATTTAATTAAAAG

mRNA sequence

ATGATTTGGGCACTTTCATTGCTTCTCAACTATGAAAAAGTGTTGAAGAAGGCTCAACTTGAATTGGACGAACAAGTTGGGAGAGGAAGACTCGTGGAAGAGTCCGACATGAAAAATTTGTCATACCTCCGAGCGATAGTGAAGGAAGTATTGCGTTTGTACCCTCCAGCACCACTCTCAACCTTTCATGAGGCAATGGAAGATTGCACCGTTGATGGATACCACGTCCCTGCTGGGACACGCATGTTGGTGAATTTTCAGAAGCTCCAAAGAGACCCGCTTGTATGGGAAGAACCATATGAATTTCGGCCAAAGAGATTTTTGACTAGTCAAAAGGATTTTGATGTTAATGGACAGAGTCCACAATTGATACCATTTGGGAGTGGTCGAAGAATATGCCCTGGAGTCTCGTTTGCCAACCAAATTATGCTCCTTACACTTGCGAACCTACTTCGAAGTATTAAAAGAATATGA

Coding sequence (CDS)

ATGATTTGGGCACTTTCATTGCTTCTCAACTATGAAAAAGTGTTGAAGAAGGCTCAACTTGAATTGGACGAACAAGTTGGGAGAGGAAGACTCGTGGAAGAGTCCGACATGAAAAATTTGTCATACCTCCGAGCGATAGTGAAGGAAGTATTGCGTTTGTACCCTCCAGCACCACTCTCAACCTTTCATGAGGCAATGGAAGATTGCACCGTTGATGGATACCACGTCCCTGCTGGGACACGCATGTTGGTGAATTTTCAGAAGCTCCAAAGAGACCCGCTTGTATGGGAAGAACCATATGAATTTCGGCCAAAGAGATTTTTGACTAGTCAAAAGGATTTTGATGTTAATGGACAGAGTCCACAATTGATACCATTTGGGAGTGGTCGAAGAATATGCCCTGGAGTCTCGTTTGCCAACCAAATTATGCTCCTTACACTTGCGAACCTACTTCGAAGTATTAAAAGAATATGA

Protein sequence

MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLSTFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQSPQLIPFGSGRRICPGVSFANQIMLLTLANLLRSIKRI

Homology

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BLAST of MC11g_new0033.1 vs. ExPASy Swiss-Prot
Match: O49396 (Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3)

HSP 1 Score: 191.0 bits (484), Expect = 9.9e-48
Identity = 96/156 (61.54%), Postives = 119/156 (76.28%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN + +LKK Q E+D  VGR R VE+SD+KNL YL+AI+KE LRLYP APL 
Sbjct: 318 LTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLL 377

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQ-KDFDVNGQ 120
              EAMEDCTV GY+VP GTR++VN  K+QRDP V+ EP EFRP+RF+T + KDFDV GQ
Sbjct: 378 GHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQ 437

Query: 121 SPQLIPFGSGRRICPGVSFANQIMLLTLANLLRSIK 156
           + +L+PFGSGRR CPG S A Q++ L LA  L S +
Sbjct: 438 NFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFE 473

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BLAST of MC11g_new0033.1 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 4.9e-47
Identity = 94/154 (61.04%), Postives = 119/154 (77.27%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN +++LKKAQ E+D  VGR R VE+SD++NL YL+AI+KE LRLYP  PL 
Sbjct: 330 LTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLL 389

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQ-KDFDVNGQ 120
              EAMEDCTV GY+VP GTR++VN  K+QRDP V+ EP EFRP+RF+T + K+FDV GQ
Sbjct: 390 GPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQ 449

Query: 121 SPQLIPFGSGRRICPGVSFANQIMLLTLANLLRS 154
           + +L+PFGSGRR CPG S A Q++ L LA  L S
Sbjct: 450 NFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHS 483

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BLAST of MC11g_new0033.1 vs. ExPASy Swiss-Prot
Match: O49394 (Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=1 SV=2)

HSP 1 Score: 184.9 bits (468), Expect = 7.1e-46
Identity = 93/154 (60.39%), Postives = 118/154 (76.62%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN + +LKKAQ E+D  VGR R VE+SD++NL Y++AI+KE LRLYP  PL 
Sbjct: 329 LTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLL 388

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQ-KDFDVNGQ 120
              EA+EDCTV GY+V  GTRMLVN  K+QRDP V+ EP EFRP+RF+T + K+FDV GQ
Sbjct: 389 GHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQ 448

Query: 121 SPQLIPFGSGRRICPGVSFANQIMLLTLANLLRS 154
           + +L+PFGSGRR CPG S A Q++ L LA  L+S
Sbjct: 449 NFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQS 482

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 3.5e-45
Identity = 89/155 (57.42%), Postives = 114/155 (73.55%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WALSLLLN    LKKAQ ELD  VGR R V+ESD+  L+Y+ AI+KE LRLYPP PL 
Sbjct: 328 LTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPLL 387

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
                 EDCT+ GYHV AGTR++VN  K+QRDPLVW +P+E++P+RFL  ++D D+ GQ 
Sbjct: 388 GPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHEYQPERFL--ERDVDMKGQH 447

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLLRSIK 156
            +LIPFGSGRR CP +S A Q++ LTLA++L   +
Sbjct: 448 FELIPFGSGRRACPAISLALQVLPLTLAHILHGFE 480

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy Swiss-Prot
Match: H2DH24 (Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 4.6e-45
Identity = 89/151 (58.94%), Postives = 106/151 (70.20%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WALSLLLN    LKKAQ ELD  VG+ R V+ESD+ NL YL AI+KE LRLYP A L 
Sbjct: 210 LTWALSLLLNNRHALKKAQEELDNVVGKQRQVKESDLNNLVYLHAIIKETLRLYPAAQLG 269

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
              E  EDCTV GYHVP GT + VN   L RDP++W +P EFRP+RFL   K+ DV GQ 
Sbjct: 270 VRREFYEDCTVAGYHVPKGTLLAVNLWTLHRDPIIWSDPTEFRPERFLNMPKEVDVKGQH 329

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 152
            +LIPFG GRR+CPG++F  Q++ L LA LL
Sbjct: 330 FELIPFGVGRRLCPGIAFGLQMLHLVLATLL 360

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BLAST of MC11g_new0033.1 vs. NCBI nr
Match: XP_038896981.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 234 bits (598), Expect = 8.48e-72
Identity = 113/151 (74.83%), Postives = 131/151 (86.75%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           MIW LSLLLN + VLKKAQLELDEQVGR + V+ESD++NL YL+AIVKE LRLYPP PL 
Sbjct: 332 MIWTLSLLLNNQDVLKKAQLELDEQVGRHKQVKESDVRNLKYLQAIVKETLRLYPPGPLG 391

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
           T HE+MEDC + GYH+PAGTR+ VN QKLQRDP VWE+P+EFRP+RFLT+ KDF+V G++
Sbjct: 392 TPHESMEDCRIAGYHIPAGTRLTVNIQKLQRDPRVWEDPHEFRPERFLTNHKDFEVRGKT 451

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFGSGRR+CP VSFA QIM LTLANLL
Sbjct: 452 PQLIPFGSGRRMCPAVSFALQIMHLTLANLL 482

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BLAST of MC11g_new0033.1 vs. NCBI nr
Match: XP_038905619.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 234 bits (597), Expect = 1.41e-71
Identity = 113/151 (74.83%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M WALSLLLN E  LKKAQLELDEQVGR RLV ESD+KNL YL+AIVKE +RLYP APL+
Sbjct: 339 MTWALSLLLNNEDTLKKAQLELDEQVGRERLVVESDVKNLLYLQAIVKETMRLYPAAPLA 398

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
            FHEAMEDC V GYH+P  TR++VN +KLQ+DPLVWE P +F+P+RFLT+ KDFDV GQS
Sbjct: 399 AFHEAMEDCNVSGYHIPRRTRLIVNLKKLQKDPLVWENPDKFQPERFLTTHKDFDVTGQS 458

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFG GRR+CPGVSF+NQ++ LTLANLL
Sbjct: 459 PQLIPFGRGRRMCPGVSFSNQVIHLTLANLL 489

[Close]

BLAST of MC11g_new0033.1 vs. NCBI nr
Match: XP_022940488.1 (cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 232 bits (591), Expect = 7.77e-71
Identity = 114/151 (75.50%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M WALSLLLN E+ LKKAQLELDEQVGR R V+ESD+KNL YL+AIVKE LRLYP APL 
Sbjct: 324 MTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIVKETLRLYPAAPLL 383

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE+MEDCTV GY++PAGTR++V+ QKLQ+DP  WE PYEFRPKRFLTSQK+ DV GQS
Sbjct: 384 VPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRFLTSQKNVDVRGQS 443

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFG+GRR+CPG+SFA Q+M LTL NLL
Sbjct: 444 PQLIPFGNGRRMCPGISFALQVMHLTLVNLL 474

[Close]

BLAST of MC11g_new0033.1 vs. NCBI nr
Match: XP_022940485.1 (cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 232 bits (591), Expect = 7.77e-71
Identity = 114/151 (75.50%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M WALSLLLN E+ LKKAQLELDEQVGR R V+ESD+KNL YL+AIVKE LRLYP APL 
Sbjct: 324 MTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIVKETLRLYPAAPLL 383

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE+MEDCTV GY++PAGTR++V+ QKLQ+DP  WE PYEFRPKRFLTSQK+ DV GQS
Sbjct: 384 VPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRFLTSQKNVDVRGQS 443

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFG+GRR+CPG+SFA Q+M LTL NLL
Sbjct: 444 PQLIPFGNGRRMCPGISFALQVMHLTLVNLL 474

[Close]

BLAST of MC11g_new0033.1 vs. NCBI nr
Match: XP_023535456.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229 bits (585), Expect = 7.86e-70
Identity = 109/151 (72.19%), Postives = 131/151 (86.75%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M+WALSLLLN+ +V+++AQLELDE VGR R V ESD+KNL YL+AIVKE LRLYP AP+ 
Sbjct: 335 MVWALSLLLNHPEVIERAQLELDEHVGRQRQVRESDVKNLLYLQAIVKETLRLYPAAPIL 394

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE++EDCTV GYH+PAGTR++VN QKLQRDP +W++P EFRP+RFLT  KDFDV GQS
Sbjct: 395 IPHESIEDCTVVGYHIPAGTRLIVNVQKLQRDPQIWKDPCEFRPERFLTDNKDFDVRGQS 454

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFGSGRR+CPG+SFA Q++ LTLANLL
Sbjct: 455 PQLIPFGSGRRMCPGISFALQVLHLTLANLL 485

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy TrEMBL
Match: A0A6J1FQP6 (cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 232 bits (591), Expect = 3.76e-71
Identity = 114/151 (75.50%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M WALSLLLN E+ LKKAQLELDEQVGR R V+ESD+KNL YL+AIVKE LRLYP APL 
Sbjct: 324 MTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIVKETLRLYPAAPLL 383

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE+MEDCTV GY++PAGTR++V+ QKLQ+DP  WE PYEFRPKRFLTSQK+ DV GQS
Sbjct: 384 VPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRFLTSQKNVDVRGQS 443

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFG+GRR+CPG+SFA Q+M LTL NLL
Sbjct: 444 PQLIPFGNGRRMCPGISFALQVMHLTLVNLL 474

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy TrEMBL
Match: A0A6J1FPG1 (cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446069 PE=3 SV=1)

HSP 1 Score: 232 bits (591), Expect = 3.76e-71
Identity = 114/151 (75.50%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M WALSLLLN E+ LKKAQLELDEQVGR R V+ESD+KNL YL+AIVKE LRLYP APL 
Sbjct: 324 MTWALSLLLNNEESLKKAQLELDEQVGRERQVQESDVKNLPYLQAIVKETLRLYPAAPLL 383

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE+MEDCTV GY++PAGTR++V+ QKLQ+DP  WE PYEFRPKRFLTSQK+ DV GQS
Sbjct: 384 VPHESMEDCTVAGYYIPAGTRLIVHAQKLQKDPSFWENPYEFRPKRFLTSQKNVDVRGQS 443

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFG+GRR+CPG+SFA Q+M LTL NLL
Sbjct: 444 PQLIPFGNGRRMCPGISFALQVMHLTLVNLL 474

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy TrEMBL
Match: A0A6J1F697 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3 SV=1)

HSP 1 Score: 229 bits (585), Expect = 3.90e-70
Identity = 109/151 (72.19%), Postives = 131/151 (86.75%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M+WALSLLLN+ +V+++AQLELDEQVGR R V E D+KNL YL+AIVKE LRLYP AP+ 
Sbjct: 336 MVWALSLLLNHPEVIERAQLELDEQVGRQRQVREFDVKNLLYLQAIVKETLRLYPAAPIL 395

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE++EDCTV GYH+PAGTR++VN QKLQRDP +W++P EFRP+RFLT  KDFDV GQS
Sbjct: 396 IPHESIEDCTVAGYHIPAGTRLIVNVQKLQRDPQIWKDPCEFRPERFLTDNKDFDVRGQS 455

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFGSGRR+CPG+SFA Q++ LTLANLL
Sbjct: 456 PQLIPFGSGRRMCPGISFALQVLHLTLANLL 486

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy TrEMBL
Match: A0A0A0LG04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1)

HSP 1 Score: 229 bits (585), Expect = 4.78e-70
Identity = 110/151 (72.85%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           MIWALSLLLN ++VLK+AQLELDE VGR R V+ESD+KNL YL+A+VKE LRLYP AP+ 
Sbjct: 344 MIWALSLLLNNQEVLKRAQLELDEHVGRQRQVKESDVKNLLYLQAVVKETLRLYPAAPIL 403

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE++EDC V GYH+P GTR++VN QKLQRDP +WE+P EF P+RFLTS+KDFDV GQS
Sbjct: 404 IPHESIEDCVVAGYHIPLGTRLIVNVQKLQRDPQIWEDPCEFHPERFLTSEKDFDVRGQS 463

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLL 151
           PQLIPFGSGRR+CPG+SFA Q+M L LANLL
Sbjct: 464 PQLIPFGSGRRMCPGISFALQVMHLALANLL 494

[Close]

BLAST of MC11g_new0033.1 vs. ExPASy TrEMBL
Match: A0A5A7STW6 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00870 PE=3 SV=1)

HSP 1 Score: 219 bits (557), Expect = 5.13e-70
Identity = 103/155 (66.45%), Postives = 129/155 (83.23%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           M WALSLLLN E  LKKAQLEL+EQVGR R V+E+D+KNL YL+AIVKE LRL+PP PL 
Sbjct: 1   MSWALSLLLNNENALKKAQLELNEQVGRARQVKETDLKNLPYLQAIVKETLRLHPPGPLL 60

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
             HE++EDCT+  +H+P GTR++VN QKLQ+DPLVW+ P EFRP+RF+T+Q +FDV GQ+
Sbjct: 61  VPHESIEDCTIGSHHIPKGTRLIVNAQKLQKDPLVWDNPCEFRPERFITNQNNFDVRGQN 120

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLLRSIK 155
           PQLIPFG+GRR+CP +SFA Q++ LTL+NLL   K
Sbjct: 121 PQLIPFGNGRRMCPAISFALQMIHLTLSNLLHGFK 155

[Close]

BLAST of MC11g_new0033.1 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 191.0 bits (484), Expect = 7.0e-49
Identity = 96/156 (61.54%), Postives = 119/156 (76.28%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN + +LKK Q E+D  VGR R VE+SD+KNL YL+AI+KE LRLYP APL 
Sbjct: 318 LTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLL 377

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQ-KDFDVNGQ 120
              EAMEDCTV GY+VP GTR++VN  K+QRDP V+ EP EFRP+RF+T + KDFDV GQ
Sbjct: 378 GHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQ 437

Query: 121 SPQLIPFGSGRRICPGVSFANQIMLLTLANLLRSIK 156
           + +L+PFGSGRR CPG S A Q++ L LA  L S +
Sbjct: 438 NFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFE 473

[Close]

BLAST of MC11g_new0033.1 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 188.7 bits (478), Expect = 3.5e-48
Identity = 94/154 (61.04%), Postives = 119/154 (77.27%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN +++LKKAQ E+D  VGR R VE+SD++NL YL+AI+KE LRLYP  PL 
Sbjct: 330 LTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLL 389

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQ-KDFDVNGQ 120
              EAMEDCTV GY+VP GTR++VN  K+QRDP V+ EP EFRP+RF+T + K+FDV GQ
Sbjct: 390 GPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQ 449

Query: 121 SPQLIPFGSGRRICPGVSFANQIMLLTLANLLRS 154
           + +L+PFGSGRR CPG S A Q++ L LA  L S
Sbjct: 450 NFELMPFGSGRRSCPGSSLAMQVLHLGLARFLHS 483

[Close]

BLAST of MC11g_new0033.1 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 184.9 bits (468), Expect = 5.0e-47
Identity = 93/154 (60.39%), Postives = 118/154 (76.62%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN + +LKKAQ E+D  VGR R VE+SD++NL Y++AI+KE LRLYP  PL 
Sbjct: 329 LTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLL 388

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQ-KDFDVNGQ 120
              EA+EDCTV GY+V  GTRMLVN  K+QRDP V+ EP EFRP+RF+T + K+FDV GQ
Sbjct: 389 GHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQ 448

Query: 121 SPQLIPFGSGRRICPGVSFANQIMLLTLANLLRS 154
           + +L+PFGSGRR CPG S A Q++ L LA  L+S
Sbjct: 449 NFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQS 482

[Close]

BLAST of MC11g_new0033.1 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 162.2 bits (409), Expect = 3.5e-40
Identity = 79/155 (50.97%), Postives = 106/155 (68.39%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           + WA+SLLLN    L+ AQ E+D  VG+GR +EESD++NL YL+AIVKE  RLYPPAPL+
Sbjct: 322 LTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKETHRLYPPAPLT 381

Query: 61  TFHEAMEDCTVDGYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNGQS 120
              EA EDC V GY V  GTR+LVN  KL RDP +W +P  F+P+RF+  +   + +  +
Sbjct: 382 GIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFMEDKSQCEKS--N 441

Query: 121 PQLIPFGSGRRICPGVSFANQIMLLTLANLLRSIK 156
            + IPFGSGRR CPGV+   +++   LA LL+  +
Sbjct: 442 FEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFE 474

[Close]

BLAST of MC11g_new0033.1 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 157.5 bits (397), Expect = 8.6e-39
Identity = 76/155 (49.03%), Postives = 104/155 (67.10%), Query Frame = 0


Query: 1   MIWALSLLLNYEKVLKKAQLELDEQVGRGRLVEESDMKNLSYLRAIVKEVLRLYPPAPLS 60
           ++WA+SLLLN   VL+KAQ ELD ++G+ R+VEE D+K+L YL+AIVKE  RLYPP PL 
Sbjct: 324 LVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVYLQAIVKETFRLYPPVPLV 383

Query: 61  TFHEAMEDCTVD--GYHVPAGTRMLVNFQKLQRDPLVWEEPYEFRPKRFLTSQKDFDVNG 120
            +   +ED  +     HVPAGT+++V+  K+ RDP VW  P +F P+RFLTS ++ DV G
Sbjct: 384 AYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPEQFEPERFLTSNRELDVGG 443

Query: 121 QSPQLIPFGSGRRICPGVSFANQIMLLTLANLLRS 154
           QS +  PFG GRR CP +    +++   L   L S
Sbjct: 444 QSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHS 478

The following BLAST results are available for this feature:

BLAST of MC11g_new0033.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49396	9.9e-48	61.54	Cytochrome P450 82C3 OS=Arabidopsis thaliana OX=3702 GN=CYP82C3 PE=2 SV=3	[more]
Q9SZ46	4.9e-47	61.04	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O49394	7.1e-46	60.39	Xanthotoxin 5-hydroxylase CYP82C2 OS=Arabidopsis thaliana OX=3702 GN=CYP82C2 PE=...	[more]
A0A0N7F297	3.5e-45	57.42	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
H2DH24	4.6e-45	58.94	Cytochrome P450 CYP82D47 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]

BLAST of MC11g_new0033.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896981.1	8.48e-72	74.83	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_038905619.1	1.41e-71	74.83	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_022940488.1	7.77e-71	75.50	cytochrome P450 CYP82D47-like isoform X2 [Cucurbita moschata]	[more]
XP_022940485.1	7.77e-71	75.50	cytochrome P450 CYP82D47-like isoform X1 [Cucurbita moschata]	[more]
XP_023535456.1	7.86e-70	72.19	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC11g_new0033.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQP6	3.76e-71	75.50	cytochrome P450 CYP82D47-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1FPG1	3.76e-71	75.50	cytochrome P450 CYP82D47-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1F697	3.90e-70	72.19	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442562 PE=3...	[more]
A0A0A0LG04	4.78e-70	72.85	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1	[more]
A0A5A7STW6	5.13e-70	66.45	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of MC11g_new0033.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31950.1	7.0e-49	61.54	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT4G31940.1	3.5e-48	61.04	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	5.0e-47	60.39	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT3G25180.1	3.5e-40	50.97	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]
AT2G25160.1	8.6e-39	49.03	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 8..28
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 1..154
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 1..154
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 10..23 score: 28.91 coord: 133..144 score: 31.13 coord: 45..56 score: 45.77 coord: 124..133 score: 58.85
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..153 e-value: 7.8E-49 score: 166.6
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 133..156 score: 27.29 coord: 123..133 score: 55.3 coord: 85..109 score: 37.44 coord: 1..27 score: 33.91 coord: 44..62 score: 32.27
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..155 e-value: 1.4E-56 score: 194.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..153
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 126..135

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
MC11g_new0033	MC11g_new0033	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
MC11g_new0033.1-cds	MC11g_new0033.1-cds-MC11:1415553..1416026	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
MC11g_new0033.1	MC11g_new0033.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen